Kcnt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnt2
Entrez Gene ID	304827
Full Name	potassium sodium-activated channel subfamily T member 2
Synonyms	Slick
General protein information	Preferred Names potassium sodium-activated channel subfamily T member 2 Names potassium channel subfamily T member 2 potassium channel, sodium-activated subfamily T, member 2 potassium channel, subfamily T, member 2 sequence like an intermediate conductance potassium channel subunit sodium and chloride-activated ATP-sensitive potassium channel Slo2.1 sodium- and chloride-activated ATP-sensitive potassium channel
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q21
Summary	a K+ channel activated by intracellular Na+ and Cl- and inhibited by intracellular ATP [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017598804.1	XP_017454293.1	potassium channel subfamily T member 2 isoform X3
XM_017598805.1	XP_017454294.1	potassium channel subfamily T member 2 isoform X6
XM_017598802.1	XP_017454291.1	potassium channel subfamily T member 2 isoform X1
XM_017598803.1	XP_017454292.1	potassium channel subfamily T member 2 isoform X2
XM_006249943.3	XP_006250005.1	potassium channel subfamily T member 2 isoform X5
XM_017598806.1	XP_017454295.1	potassium channel subfamily T member 2 isoform X7
XM_006249942.3	XP_006250004.1	potassium channel subfamily T member 2 isoform X4
NM_198762.1	NP_942057.1	potassium channel subfamily T member 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Kcnt2	NP_942057.1
Canis lupus familiaris (dog)	KCNT2	XP_849284.2
Danio rerio (zebrafish)	LOC565691	XP_005164308.1
Pan troglodytes (chimpanzee)	KCNT2	XP_001134868.1
	KCNT2	XP_001112100.1
Bos taurus (cattle)	KCNT2	XP_003587097.2
Homo sapiens (human)	KCNT2	NP_940905.2
Mus musculus (house mouse)	Kcnt2	NP_001074496.2
Gallus gallus (chicken)	KCNT2	XP_426614.3
Xenopus tropicalis (tropical clawed frog)	kcnt1	XP_004918922.1

Gene Ontology
Bibliography

Related articles in PubMed

A De Novo Mutation in the Sodium-Activated Potassium Channel KCNT2 Alters Ion Selectivity and Causes Epileptic Encephalopathy.
Gururaj S, Palmer EE, Sheehan GD, Kandula T, Macintosh R, Ying K, Morris P, Tao J, Dias KR, Zhu Y, Dinger ME, Cowley MJ, Kirk EP, Roscioli T, Sachdev R, Duffey ME, Bye A, Bhattacharjee A
Cell reports21(4)926-933(2017 Oct)

SLO2 Channels Are Inhibited by All Divalent Cations That Activate SLO1 K+ Channels.
Budelli G, Sun Q, Ferreira J, Butler A, Santi CM, Salkoff L
The Journal of biological chemistry291(14)7347-56(2016 Apr)

Transcriptional Regulation of the Sodium-activated Potassium Channel SLICK (KCNT2) Promoter by Nuclear Factor-?B.
Tomasello DL, Gancarz-Kausch AM, Dietz DM, Bhattacharjee A
The Journal of biological chemistry290(30)18575-83(2015 Jul)

Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels.
Tejada MA, Stople K, Hammami Bomholtz S, Meinild AK, Poulsen AN, Klaerke DA
PloS one9(10)e110833(2014)

The N-terminal domain of Slack determines the formation and trafficking of Slick/Slack heteromeric sodium-activated potassium channels.
Chen H, Kronengold J, Yan Y, Gazula VR, Brown MR, Ma L, Ferreira G, Yang Y, Bhattacharjee A, Sigworth FJ, Salkoff L, Kaczmarek LK
The Journal of neuroscience : the official journal of the Society for Neuroscience29(17)5654-65(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SLO2 Channels Are Inhibited by All Divalent Cations That Activate SLO1 K+ Channels
Title: SLO2 Channels Are Inhibited by All Divalent Cations That Activate SLO1 K+ Channels.

Our data show SLICK expression is predominantly under the control of NFkappaB. Because neuronal NFkappaB activation occurs during stressful stimuli such as hypoxia and injury, our findings suggest that SLICK is a neuroprotective gene.
Title: Transcriptional Regulation of the Sodium-activated Potassium Channel SLICK (KCNT2) Promoter by Nuclear Factor-?B.

Slick channels, in contrast to the similar Slack channels, are the only high-conductance K+ channels strongly sensitive to small changes in cell volume.
Title: Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels.

Heteromer formation between Slick and Slack-B requires a specific epitope that is found in the N-terminal domain of Slack-B and that is absent in the Slack-A isoform.
Title: The N-terminal domain of Slack determines the formation and trafficking of Slick/Slack heteromeric sodium-activated potassium channels.

We conclude that Slack and Slick K(Na) channels are required for depolarizing afterpotential of medium diameter rat dorsal root ganglion neurons
Title: Slack and Slick KNa channels are required for the depolarizing afterpotential of acutely isolated, medium diameter rat dorsal root ganglion neurons.

The following Kcnt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa58070	XM_017598805.1 Latest version!	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa58067	XM_017598802.1 Latest version!	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa58068	XM_017598803.1 Latest version!	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa58069	XM_017598804.1 Latest version!	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa58071	XM_017598806.1 Latest version!	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa04991	NM_198762.1 Latest version!	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa35408	XM_006249942.3 Latest version!	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa35409	XM_006249943.3 Latest version!	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnt2 ( XM_017598805.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454294.1 Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa58070
Clone ID Related Accession (Same CDS sequence)	XM_017598805.1
Accession Version	XM_017598805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium channel subfamily T member 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGTT TTACAGACAA AACAAGAGAG 
CACATGTATC ATGTGACTTT GGGATTTACT GAAACTTTGA CATTTTCTCA AAGCATTTGT 
GGAATTCAGC ATCTGGAACG AATTGGAAAG AAACTCAATC TCTTTGACTC CCTTTATTTC 
TGCATTGTGA CATTTTCTAC TGTGGGCTTT GGGGATGTTA CTCCTGAAAC ATGGTCCTCC 
AAGCTTTTTG TTGTTGCTAT GATCTGCGTT GCTCTTGTAG TTCTCCCCAT ACAGTTTGAA 
CAGCTGGCCT ATTTGTGGAT GGAGAGACAA AAGTCGGGAG GAAACTATAG TCGACACAGA 
GCTCAGACTG AGAAGCATGT TGTTCTGTGT GTCAGCTCAT TGAAGATTGA CTTGCTTATG 
GATTTTTTGA ATGAATTCTA TGCACATCCA AGACTGCAGG ATTATTATGT GGTGATTTTG 
TGTCCCACGG AGATGGATGT GCAAGTTCGG AGAGTGCTAC AGATTCCAAT GTGGTCCCAG 
AGAGTGATCT ACCTTCAAGG CTCTGCCCTT AAAGACCAGG ATCTCCTGAG AGCAAAGATG 
GACAATGCTG AGGCCTGTTT CATTTTGAGT AGCCGCTGTG AAGTCGATAG AACATCATCT 
GATCACCAGA CAATTTTGAG AGCATGGGCT GTAAAAGATT TTGCCCCAAA TTGTCCTCTG 
TATGTTCAGA TATTAAAACC AGAAAATAAA TTCCACATCA AATTTGCAGA TCATGTTGTT 
TGTGAAGAAG AGTTTAAGTA CGCCATGTTA GCTTTAAACT GTATATGCCC AGCAACATCT 
ACACTTATTA CACTACTGGT TCATACCTCT AGAGGGCAAG AAGGGCAGCA GTCACCTGAA 
CAGTGGCAGA AGACGTATGG GAGGTGCTCA GGAAATGAAG TCTACCACAT CGTTCTGGAA 
GAAAGTACAT TTTTTGCTGA ATATGAAGGG AAGAGTTTTA CCTATGCTTC TTTCCATGCA 
CACAAAAAGT TTGGTGTCTG CTTGGTTGGT GTTAGGAGGG AGGATAATAA AAACATTTTG 
CTGAATCCAG GTCCTCGATA CATTATGAAT GCTTCAGACA TATGTTTTTA TATTAATATT 
ACCAAAGAAG AAAATTCAGC ATTTAAGAAT CAAGACCAAC AGAGAAAAAG TAATGTGTCA 
AGGTCATTTT ATCATGGGCC TTCCAGATTG CCTGTCCACA GTATCATTGC CAGCATGGGT 
ACTGTGGCTA TAGACTTACA AGACACAAGC TGTAGAGCAG CAAGTGGCCC TACACTGGCT 
CTTCCTTCAG AGGGAGGCAA GGAACTAAGA AGACCTAGCA TTGCTCCTGT TTTAGAGGTA 
GCAGATACAT CATCAATTCA AACCTGCGAT CTTCTAAGTG ACCAGTCAGA AGATGAAACT 
ACACCAGATG AAGAAACATC TTCAAATTTA GAGTATGCCA AAGGCTACCC ACCTTATTCT 
CCATACATAG GAAGTTCACC TACTTTTTGT CATCTACTTC AAGAAAAAGT GCCCTTTTGC 
TGCTTAAGAT TAGACAAGAG TTGCCAGCAT AACTACTATG AGGATGCAAA AGCCTATGGA 
TTCAAAAATA AACTAATTAT AGTTGCAGCT GAAACGGCTG GGAACGGATT ATATAATTTT 
ATTGTACCTC TCAGGGCATA TTATAGACCA AAGAAAGAAT TAAATCCCAT AGTTCTTCTA 
TTGGATAATC CGCCAGATAT GCATTTTCTG GATGCAATCT GTTGGTTTCC AATGGTTTAC 
TACATGGTGG GCTCTATTGA CAACCTAGAT GATTTGCTCA GGTGTGGAGT GACCTTCGCT 
GCCAACATGG TGGTTGTGGA CAAAGAAAGC ACCATGAGTG CAGAGGAAGA CTACATGGCA 
GATGCCAAGA CGATTGTGAA TGTGCAGACT CTTTTCAGGT TGTTTTCGAG TCTCAGTATT 
ATCACTGAGC TTACTCATCC AGCAAATATG AGATTCATGC AATTCAGAGC CAAGGACTGT 
TACTCTCTTG CACTTTCAAA ACTGGAAAAG AAAGAGCGAG AACGAGGTTC TAACCTGGCC 
TTCATGTTCC GACTGCCTTT CGCTGCGGGG AGAGTGTTCA GCATCAGTAT GCTGGACACG 
CTGCTTTATC AGACTTCTTT TGGGACTGGA CACCATACCA GGATCGGGGT TTCTTTGTTC 
TATGAAAATT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454294.1
CDS	579..3413
Translation	MVDLESEVPPLPPRYRFRDLLLGDQGWQNDDRVQVEFYMNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLSCLLYIIRVLLEKPSQGNDWSHIFWVNRSLPLWGLQVSVALISLFETILLGYLSYKGNIWEQILRVPFILEIINAVPFIISIFWPTLRNLFVPVFLNCWLAKHALENMINDLHRAIQRTQSAMFNQVLILISTLLCLIFTCFTDKTREHMYHVTLGFTETLTFSQSICGIQHLERIGKKLNLFDSLYFCIVTFSTVGFGDVTPETWSSKLFVVAMICVALVVLPIQFEQLAYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDVQVRRVLQIPMWSQRVIYLQGSALKDQDLLRAKMDNAEACFILSSRCEVDRTSSDHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCICPATSTLITLLVHTSRGQEGQQSPEQWQKTYGRCSGNEVYHIVLEESTFFAEYEGKSFTYASFHAHKKFGVCLVGVRREDNKNILLNPGPRYIMNASDICFYINITKEENSAFKNQDQQRKSNVSRSFYHGPSRLPVHSIIASMGTVAIDLQDTSCRAASGPTLALPSEGGKELRRPSIAPVLEVADTSSIQTCDLLSDQSEDETTPDEETSSNLEYAKGYPPYSPYIGSSPTFCHLLQEKVPFCCLRLDKSCQHNYYEDAKAYGFKNKLIIVAAETAGNGLYNFIVPLRAYYRPKKELNPIVLLLDNPPDMHFLDAICWFPMVYYMVGSIDNLDDLLRCGVTFAANMVVVDKESTMSAEEDYMADAKTIVNVQTLFRLFSSLSIITELTHPANMRFMQFRAKDCYSLALSKLEKKERERGSNLAFMFRLPFAAGRVFSISMLDTLLYQTSFGTGHHTRIGVSLFYENY

Target ORF information:

RefSeq Version	XM_017598805.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598805.1

ORF Insert Sequence:

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGTT TTACAGACAA AACAAGAGAG 
CACATGTATC ATGTGACTTT GGGATTTACT GAAACTTTGA CATTTTCTCA AAGCATTTGT 
GGAATTCAGC ATCTGGAACG AATTGGAAAG AAACTCAATC TCTTTGACTC CCTTTATTTC 
TGCATTGTGA CATTTTCTAC TGTGGGCTTT GGGGATGTTA CTCCTGAAAC ATGGTCCTCC 
AAGCTTTTTG TTGTTGCTAT GATCTGCGTT GCTCTTGTAG TTCTCCCCAT ACAGTTTGAA 
CAGCTGGCCT ATTTGTGGAT GGAGAGACAA AAGTCGGGAG GAAACTATAG TCGACACAGA 
GCTCAGACTG AGAAGCATGT TGTTCTGTGT GTCAGCTCAT TGAAGATTGA CTTGCTTATG 
GATTTTTTGA ATGAATTCTA TGCACATCCA AGACTGCAGG ATTATTATGT GGTGATTTTG 
TGTCCCACGG AGATGGATGT GCAAGTTCGG AGAGTGCTAC AGATTCCAAT GTGGTCCCAG 
AGAGTGATCT ACCTTCAAGG CTCTGCCCTT AAAGACCAGG ATCTCCTGAG AGCAAAGATG 
GACAATGCTG AGGCCTGTTT CATTTTGAGT AGCCGCTGTG AAGTCGATAG AACATCATCT 
GATCACCAGA CAATTTTGAG AGCATGGGCT GTAAAAGATT TTGCCCCAAA TTGTCCTCTG 
TATGTTCAGA TATTAAAACC AGAAAATAAA TTCCACATCA AATTTGCAGA TCATGTTGTT 
TGTGAAGAAG AGTTTAAGTA CGCCATGTTA GCTTTAAACT GTATATGCCC AGCAACATCT 
ACACTTATTA CACTACTGGT TCATACCTCT AGAGGGCAAG AAGGGCAGCA GTCACCTGAA 
CAGTGGCAGA AGACGTATGG GAGGTGCTCA GGAAATGAAG TCTACCACAT CGTTCTGGAA 
GAAAGTACAT TTTTTGCTGA ATATGAAGGG AAGAGTTTTA CCTATGCTTC TTTCCATGCA 
CACAAAAAGT TTGGTGTCTG CTTGGTTGGT GTTAGGAGGG AGGATAATAA AAACATTTTG 
CTGAATCCAG GTCCTCGATA CATTATGAAT GCTTCAGACA TATGTTTTTA TATTAATATT 
ACCAAAGAAG AAAATTCAGC ATTTAAGAAT CAAGACCAAC AGAGAAAAAG TAATGTGTCA 
AGGTCATTTT ATCATGGGCC TTCCAGATTG CCTGTCCACA GTATCATTGC CAGCATGGGT 
ACTGTGGCTA TAGACTTACA AGACACAAGC TGTAGAGCAG CAAGTGGCCC TACACTGGCT 
CTTCCTTCAG AGGGAGGCAA GGAACTAAGA AGACCTAGCA TTGCTCCTGT TTTAGAGGTA 
GCAGATACAT CATCAATTCA AACCTGCGAT CTTCTAAGTG ACCAGTCAGA AGATGAAACT 
ACACCAGATG AAGAAACATC TTCAAATTTA GAGTATGCCA AAGGCTACCC ACCTTATTCT 
CCATACATAG GAAGTTCACC TACTTTTTGT CATCTACTTC AAGAAAAAGT GCCCTTTTGC 
TGCTTAAGAT TAGACAAGAG TTGCCAGCAT AACTACTATG AGGATGCAAA AGCCTATGGA 
TTCAAAAATA AACTAATTAT AGTTGCAGCT GAAACGGCTG GGAACGGATT ATATAATTTT 
ATTGTACCTC TCAGGGCATA TTATAGACCA AAGAAAGAAT TAAATCCCAT AGTTCTTCTA 
TTGGATAATC CGCCAGATAT GCATTTTCTG GATGCAATCT GTTGGTTTCC AATGGTTTAC 
TACATGGTGG GCTCTATTGA CAACCTAGAT GATTTGCTCA GGTGTGGAGT GACCTTCGCT 
GCCAACATGG TGGTTGTGGA CAAAGAAAGC ACCATGAGTG CAGAGGAAGA CTACATGGCA 
GATGCCAAGA CGATTGTGAA TGTGCAGACT CTTTTCAGGT TGTTTTCGAG TCTCAGTATT 
ATCACTGAGC TTACTCATCC AGCAAATATG AGATTCATGC AATTCAGAGC CAAGGACTGT 
TACTCTCTTG CACTTTCAAA ACTGGAAAAG AAAGAGCGAG AACGAGGTTC TAACCTGGCC 
TTCATGTTCC GACTGCCTTT CGCTGCGGGG AGAGTGTTCA GCATCAGTAT GCTGGACACG 
CTGCTTTATC AGACTTCTTT TGGGACTGGA CACCATACCA GGATCGGGGT TTCTTTGTTC 
TATGAAAATT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt2 ( XM_017598802.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454291.1 Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58067
Clone ID Related Accession (Same CDS sequence)	XM_017598802.1
Accession Version	XM_017598802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium channel subfamily T member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGTT TTACAGACAA AACAAGAGAG 
CACATGTATC ATGTGACTTT GGGATTTACT GAAACTTTGA CATTTTCTCA AAGCATTTGT 
GGAATTCAGC ATCTGGAACG AATTGGAAAG AAACTCAATC TCTTTGACTC CCTTTATTTC 
TGCATTGTGA CATTTTCTAC TGTGGGCTTT GGGGATGTTA CTCCTGAAAC ATGGTCCTCC 
AAGCTTTTTG TTGTTGCTAT GATCTGCGTT GCTCTTGTAG TTCTCCCCAT ACAGTTTGAA 
CAGCTGGCCT ATTTGTGGAT GGAGAGACAA AAGTCGGGAG GAAACTATAG TCGACACAGA 
GCTCAGACTG AGAAGCATGT TGTTCTGTGT GTCAGCTCAT TGAAGATTGA CTTGCTTATG 
GATTTTTTGA ATGAATTCTA TGCACATCCA AGACTGCAGG ATTATTATGT GGTGATTTTG 
TGTCCCACGG AGATGGATGT GCAAGTTCGG AGAGTGCTAC AGATTCCAAT GTGGTCCCAG 
AGAGTGATCT ACCTTCAAGG CTCTGCCCTT AAAGACCAGG ATCTCCTGAG AGCAAAGATG 
GACAATGCTG AGGCCTGTTT CATTTTGAGT AGCCGCTGTG AAGTCGATAG AACATCATCT 
GATCACCAGA CAATTTTGAG AGCATGGGCT GTAAAAGATT TTGCCCCAAA TTGTCCTCTG 
TATGTTCAGA TATTAAAACC AGAAAATAAA TTCCACATCA AATTTGCAGA TCATGTTGTT 
TGTGAAGAAG AGTTTAAGTA CGCCATGTTA GCTTTAAACT GTATATGCCC AGCAACATCT 
ACACTTATTA CACTACTGGT TCATACCTCT AGAGGGCAAG AAGGGCAGCA GTCACCTGAA 
CAGTGGCAGA AGACGTATGG GAGGTGCTCA GGAAATGAAG TCTACCACAT CGTTCTGGAA 
GAAAGTACAT TTTTTGCTGA ATATGAAGGG AAGAGTTTTA CCTATGCTTC TTTCCATGCA 
CACAAAAAGT TTGGTGTCTG CTTGGTTGGT GTTAGGAGGG AGGATAATAA AAACATTTTG 
CTGAATCCAG GTCCTCGATA CATTATGAAT GCTTCAGACA TATGTTTTTA TATTAATATT 
ACCAAAGAAG AAAATTCAGC ATTTAAGAAT CAAGACCAAC AGAGAAAAAG TAATGTGTCA 
AGGTCATTTT ATCATGGGCC TTCCAGATTG CCTGTCCACA GTATCATTGC CAGCATGGGT 
ACTGTGGCTA TAGACTTACA AGACACAAGC TGTAGAGCAG CAAGTGGCCC TACACTGGCT 
CTTCCTTCAG AGGGAGGCAA GGAACTAAGA AGACCTAGCA TTGCTCCTGT TTTAGAGGTA 
GCAGATACAT CATCAATTCA AACCTGCGAT CTTCTAAGTG ACCAGTCAGA AGATGAAACT 
ACACCAGATG AAGAAACATC TTCAAATTTA GAGTATGCCA AAGGCTACCC ACCTTATTCT 
CCATACATAG GAAGTTCACC TACTTTTTGT CATCTACTTC AAGAAAAAGT GCCCTTTTGC 
TGCTTAAGAT TAGACAAGAG TTGCCAGCAT AACTACTATG AGGATGCAAA AGCCTATGGA 
TTCAAAAATA AACTAATTAT AGTTGCAGCT GAAACGGCTG GGAACGGATT ATATAATTTT 
ATTGTACCTC TCAGGGCATA TTATAGACCA AAGAAAGAAT TAAATCCCAT AGTTCTTCTA 
TTGGATAATC CGCCAGATAT GCATTTTCTG GATGCAATCT GTTGGTTTCC AATGGTTTAC 
TACATGGTGG GCTCTATTGA CAACCTAGAT GATTTGCTCA GGTGTGGAGT GACCTTCGCT 
GCCAACATGG TGGTTGTGGA CAAAGAAAGC ACCATGAGTG CAGAGGAAGA CTACATGGCA 
GATGCCAAGA CGATTGTGAA TGTGCAGACT CTTTTCAGGT TGTTTTCGAG TCTCAGTATT 
ATCACTGAGC TTACTCATCC AGCAAATATG AGATTCATGC AATTCAGAGC CAAGGACTGT 
TACTCTCTTG CACTTTCAAA ACTGGAAAAG AAAGAGCGAG AACGAGGTTC TAACCTGGCC 
TTCATGTTCC GACTGCCTTT CGCTGCGGGG AGAGTGTTCA GCATCAGTAT GCTGGACACG 
CTGCTTTATC AGTCATTTGT GAAAGATTAT ATGATTTCTA TCACCAGACT TCTTTTGGGA 
CTGGACACCA TACCAGGATC GGGGTTTCTT TGTTCTATGA AAATTACTGA AGATGACTTG 
TGGATCAGAA CGTATGCCAG ACTCTATCAG AAGCTGTGTT CTTCTACTGG AGATGTTCCC 
ATTGGGATCT ACAGAACAGA ATCTCAGAAG CTAACTACAT CTGAGTCTCG AGAAATAGCA 
TCTCAATCTC AAATATCCAT CAGTGTGGAA GAGTGGGAAG ACACCAAAGA TGTCAAAGAC 
CCAGGGCACC ACCGCAGCAT TCACCGCAAC TCAACCTCTA GCGACCAGTC AGACCACCCC 
TTGCTGCGGA GGAAGAGCAT GCAGTGGGCC CGGAGGCTGA GCAGAAAAGG GCCAAAGCAC 
TCTGGTAAAA CTGCAGAAAA GATAACCCAG CAACGACTGA ATCTCTACAG GAGGTCGGAA 
AGACAGGAGC TTGCTGAACT TGTGAAAAAC AGAATGAAAC ACCTGGGCCT CTCTACGGTG 
GGCTACGATG AAATGAATGA TCATCAGAGC ACCCTTTCCT ACATCCTGAT TAATCCATCT 
CCAGACACCA GGCTTGAGCT GAATGATGTT GTATATTTGA TCCGACCAGA TCCACTGTCC 
TACCTTCCGA ACAGTGAACC CAGTAGGAAA AACAGCATCT GCAATGCCGC TGTTCAAGAT 
TCTCGGGAGG AAACACAACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454291.1
CDS	579..4082
Translation	MVDLESEVPPLPPRYRFRDLLLGDQGWQNDDRVQVEFYMNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLSCLLYIIRVLLEKPSQGNDWSHIFWVNRSLPLWGLQVSVALISLFETILLGYLSYKGNIWEQILRVPFILEIINAVPFIISIFWPTLRNLFVPVFLNCWLAKHALENMINDLHRAIQRTQSAMFNQVLILISTLLCLIFTCFTDKTREHMYHVTLGFTETLTFSQSICGIQHLERIGKKLNLFDSLYFCIVTFSTVGFGDVTPETWSSKLFVVAMICVALVVLPIQFEQLAYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDVQVRRVLQIPMWSQRVIYLQGSALKDQDLLRAKMDNAEACFILSSRCEVDRTSSDHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCICPATSTLITLLVHTSRGQEGQQSPEQWQKTYGRCSGNEVYHIVLEESTFFAEYEGKSFTYASFHAHKKFGVCLVGVRREDNKNILLNPGPRYIMNASDICFYINITKEENSAFKNQDQQRKSNVSRSFYHGPSRLPVHSIIASMGTVAIDLQDTSCRAASGPTLALPSEGGKELRRPSIAPVLEVADTSSIQTCDLLSDQSEDETTPDEETSSNLEYAKGYPPYSPYIGSSPTFCHLLQEKVPFCCLRLDKSCQHNYYEDAKAYGFKNKLIIVAAETAGNGLYNFIVPLRAYYRPKKELNPIVLLLDNPPDMHFLDAICWFPMVYYMVGSIDNLDDLLRCGVTFAANMVVVDKESTMSAEEDYMADAKTIVNVQTLFRLFSSLSIITELTHPANMRFMQFRAKDCYSLALSKLEKKERERGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMISITRLLLGLDTIPGSGFLCSMKITEDDLWIRTYARLYQKLCSSTGDVPIGIYRTESQKLTTSESREIASQSQISISVEEWEDTKDVKDPGHHRSIHRNSTSSDQSDHPLLRRKSMQWARRLSRKGPKHSGKTAEKITQQRLNLYRRSERQELAELVKNRMKHLGLSTVGYDEMNDHQSTLSYILINPSPDTRLELNDVVYLIRPDPLSYLPNSEPSRKNSICNAAVQDSREETQL

Target ORF information:

RefSeq Version	XM_017598802.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598802.1

ORF Insert Sequence:

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGTT TTACAGACAA AACAAGAGAG 
CACATGTATC ATGTGACTTT GGGATTTACT GAAACTTTGA CATTTTCTCA AAGCATTTGT 
GGAATTCAGC ATCTGGAACG AATTGGAAAG AAACTCAATC TCTTTGACTC CCTTTATTTC 
TGCATTGTGA CATTTTCTAC TGTGGGCTTT GGGGATGTTA CTCCTGAAAC ATGGTCCTCC 
AAGCTTTTTG TTGTTGCTAT GATCTGCGTT GCTCTTGTAG TTCTCCCCAT ACAGTTTGAA 
CAGCTGGCCT ATTTGTGGAT GGAGAGACAA AAGTCGGGAG GAAACTATAG TCGACACAGA 
GCTCAGACTG AGAAGCATGT TGTTCTGTGT GTCAGCTCAT TGAAGATTGA CTTGCTTATG 
GATTTTTTGA ATGAATTCTA TGCACATCCA AGACTGCAGG ATTATTATGT GGTGATTTTG 
TGTCCCACGG AGATGGATGT GCAAGTTCGG AGAGTGCTAC AGATTCCAAT GTGGTCCCAG 
AGAGTGATCT ACCTTCAAGG CTCTGCCCTT AAAGACCAGG ATCTCCTGAG AGCAAAGATG 
GACAATGCTG AGGCCTGTTT CATTTTGAGT AGCCGCTGTG AAGTCGATAG AACATCATCT 
GATCACCAGA CAATTTTGAG AGCATGGGCT GTAAAAGATT TTGCCCCAAA TTGTCCTCTG 
TATGTTCAGA TATTAAAACC AGAAAATAAA TTCCACATCA AATTTGCAGA TCATGTTGTT 
TGTGAAGAAG AGTTTAAGTA CGCCATGTTA GCTTTAAACT GTATATGCCC AGCAACATCT 
ACACTTATTA CACTACTGGT TCATACCTCT AGAGGGCAAG AAGGGCAGCA GTCACCTGAA 
CAGTGGCAGA AGACGTATGG GAGGTGCTCA GGAAATGAAG TCTACCACAT CGTTCTGGAA 
GAAAGTACAT TTTTTGCTGA ATATGAAGGG AAGAGTTTTA CCTATGCTTC TTTCCATGCA 
CACAAAAAGT TTGGTGTCTG CTTGGTTGGT GTTAGGAGGG AGGATAATAA AAACATTTTG 
CTGAATCCAG GTCCTCGATA CATTATGAAT GCTTCAGACA TATGTTTTTA TATTAATATT 
ACCAAAGAAG AAAATTCAGC ATTTAAGAAT CAAGACCAAC AGAGAAAAAG TAATGTGTCA 
AGGTCATTTT ATCATGGGCC TTCCAGATTG CCTGTCCACA GTATCATTGC CAGCATGGGT 
ACTGTGGCTA TAGACTTACA AGACACAAGC TGTAGAGCAG CAAGTGGCCC TACACTGGCT 
CTTCCTTCAG AGGGAGGCAA GGAACTAAGA AGACCTAGCA TTGCTCCTGT TTTAGAGGTA 
GCAGATACAT CATCAATTCA AACCTGCGAT CTTCTAAGTG ACCAGTCAGA AGATGAAACT 
ACACCAGATG AAGAAACATC TTCAAATTTA GAGTATGCCA AAGGCTACCC ACCTTATTCT 
CCATACATAG GAAGTTCACC TACTTTTTGT CATCTACTTC AAGAAAAAGT GCCCTTTTGC 
TGCTTAAGAT TAGACAAGAG TTGCCAGCAT AACTACTATG AGGATGCAAA AGCCTATGGA 
TTCAAAAATA AACTAATTAT AGTTGCAGCT GAAACGGCTG GGAACGGATT ATATAATTTT 
ATTGTACCTC TCAGGGCATA TTATAGACCA AAGAAAGAAT TAAATCCCAT AGTTCTTCTA 
TTGGATAATC CGCCAGATAT GCATTTTCTG GATGCAATCT GTTGGTTTCC AATGGTTTAC 
TACATGGTGG GCTCTATTGA CAACCTAGAT GATTTGCTCA GGTGTGGAGT GACCTTCGCT 
GCCAACATGG TGGTTGTGGA CAAAGAAAGC ACCATGAGTG CAGAGGAAGA CTACATGGCA 
GATGCCAAGA CGATTGTGAA TGTGCAGACT CTTTTCAGGT TGTTTTCGAG TCTCAGTATT 
ATCACTGAGC TTACTCATCC AGCAAATATG AGATTCATGC AATTCAGAGC CAAGGACTGT 
TACTCTCTTG CACTTTCAAA ACTGGAAAAG AAAGAGCGAG AACGAGGTTC TAACCTGGCC 
TTCATGTTCC GACTGCCTTT CGCTGCGGGG AGAGTGTTCA GCATCAGTAT GCTGGACACG 
CTGCTTTATC AGTCATTTGT GAAAGATTAT ATGATTTCTA TCACCAGACT TCTTTTGGGA 
CTGGACACCA TACCAGGATC GGGGTTTCTT TGTTCTATGA AAATTACTGA AGATGACTTG 
TGGATCAGAA CGTATGCCAG ACTCTATCAG AAGCTGTGTT CTTCTACTGG AGATGTTCCC 
ATTGGGATCT ACAGAACAGA ATCTCAGAAG CTAACTACAT CTGAGTCTCG AGAAATAGCA 
TCTCAATCTC AAATATCCAT CAGTGTGGAA GAGTGGGAAG ACACCAAAGA TGTCAAAGAC 
CCAGGGCACC ACCGCAGCAT TCACCGCAAC TCAACCTCTA GCGACCAGTC AGACCACCCC 
TTGCTGCGGA GGAAGAGCAT GCAGTGGGCC CGGAGGCTGA GCAGAAAAGG GCCAAAGCAC 
TCTGGTAAAA CTGCAGAAAA GATAACCCAG CAACGACTGA ATCTCTACAG GAGGTCGGAA 
AGACAGGAGC TTGCTGAACT TGTGAAAAAC AGAATGAAAC ACCTGGGCCT CTCTACGGTG 
GGCTACGATG AAATGAATGA TCATCAGAGC ACCCTTTCCT ACATCCTGAT TAATCCATCT 
CCAGACACCA GGCTTGAGCT GAATGATGTT GTATATTTGA TCCGACCAGA TCCACTGTCC 
TACCTTCCGA ACAGTGAACC CAGTAGGAAA AACAGCATCT GCAATGCCGC TGTTCAAGAT 
TCTCGGGAGG AAACACAACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt2 ( XM_017598803.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454292.1 Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X2, mRNA.

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CloneID	ORa58068
Clone ID Related Accession (Same CDS sequence)	XM_017598803.1
Accession Version	XM_017598803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium channel subfamily T member 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGTT TTACAGACAA AACAAGAGAG 
CACATGTATC ATGTGACTTT GGGATTTACT GAAACTTTGA CATTTTCTCA AAGCATTTGT 
GGAATTCAGC ATCTGGAACG AATTGGAAAG AAACTCAATC TCTTTGACTC CCTTTATTTC 
TGCATTGTGA CATTTTCTAC TGTGGGCTTT GGGGATGTTA CTCCTGAAAC ATGGTCCTCC 
AAGCTTTTTG TTGTTGCTAT GATCTGCGTT GCTCTTGTAG TTCTCCCCAT ACAGTTTGAA 
CAGCTGGCCT ATTTGTGGAT GGAGAGACAA AAGTCGGGAG GAAACTATAG TCGACACAGA 
GCTCAGACTG AGAAGCATGT TGTTCTGTGT GTCAGCTCAT TGAAGATTGA CTTGCTTATG 
GATTTTTTGA ATGAATTCTA TGCACATCCA AGACTGCAGG ATTATTATGT GGTGATTTTG 
TGTCCCACGG AGATGGATGT GCAAGTTCGG AGAGTGCTAC AGATTCCAAT GTGGTCCCAG 
AGAGTGATCT ACCTTCAAGG CTCTGCCCTT AAAGACCAGG ATCTCCTGAG AGCAAAGATG 
GACAATGCTG AGGCCTGTTT CATTTTGAGT AGCCGCTGTG AAGTCGATAG AACATCATCT 
GATCACCAGA CAATTTTGAG AGCATGGGCT GTAAAAGATT TTGCCCCAAA TTGTCCTCTG 
TATGTTCAGA TATTAAAACC AGAAAATAAA TTCCACATCA AATTTGCAGA TCATGTTGTT 
TGTGAAGAAG AGTTTAAGTA CGCCATGTTA GCTTTAAACT GTATATGCCC AGCAACATCT 
ACACTTATTA CACTACTGGT TCATACCTCT AGAGGGCAAG AAGGGCAGCA GTCACCTGAA 
CAGTGGCAGA AGACGTATGG GAGGTGCTCA GGAAATGAAG TCTACCACAT CGTTCTGGAA 
GAAAGTACAT TTTTTGCTGA ATATGAAGGG AAGAGTTTTA CCTATGCTTC TTTCCATGCA 
CACAAAAAGT TTGGTGTCTG CTTGGTTGGT GTTAGGAGGG AGGATAATAA AAACATTTTG 
CTGAATCCAG GTCCTCGATA CATTATGAAT GCTTCAGACA TATGTTTTTA TATTAATATT 
ACCAAAGAAG AAAATTCAGC ATTTAAGAAT CAAGACCAAC AGAGAAAAAG TAATGTGTCA 
AGGTCATTTT ATCATGGGCC TTCCAGATTG CCTGTCCACA GTATCATTGC CAGCATGGGT 
ACTGTGGCTA TAGACTTACA AGACACAAGC TGTAGAGCAG CAAGTGGCCC TACACTGGCT 
CTTCCTTCAG AGGGAGGCAA GGAACTAAGA AGACCTAGCA TTGCTCCTGT TTTAGAGGTA 
GCAGATACAT CATCAATTCA AACCTGCGAT CTTCTAAGTG ACCAGTCAGA AGATGAAACT 
ACACCAGATG AAGAAACATC TTCAAATTTA GAGTATGCCA AAGGCTACCC ACCTTATTCT 
CCATACATAG GAAGTTCACC TACTTTTTGT CATCTACTTC AAGAAAAAGT GCCCTTTTGC 
TGCTTAAGAT TAGACAAGAG TTGCCAGCAT AACTACTATG AGGATGCAAA AGCCTATGGA 
TTCAAAAATA AACTAATTAT AGTTGCAGCT GAAACGGCTG GGAACGGATT ATATAATTTT 
ATTGTACCTC TCAGGGCATA TTATAGACCA AAGAAAGAAT TAAATCCCAT AGTTCTTCTA 
TTGGATAATC CCCTAGATGA TTTGCTCAGG TGTGGAGTGA CCTTCGCTGC CAACATGGTG 
GTTGTGGACA AAGAAAGCAC CATGAGTGCA GAGGAAGACT ACATGGCAGA TGCCAAGACG 
ATTGTGAATG TGCAGACTCT TTTCAGGTTG TTTTCGAGTC TCAGTATTAT CACTGAGCTT 
ACTCATCCAG CAAATATGAG ATTCATGCAA TTCAGAGCCA AGGACTGTTA CTCTCTTGCA 
CTTTCAAAAC TGGAAAAGAA AGAGCGAGAA CGAGGTTCTA ACCTGGCCTT CATGTTCCGA 
CTGCCTTTCG CTGCGGGGAG AGTGTTCAGC ATCAGTATGC TGGACACGCT GCTTTATCAG 
TCATTTGTGA AAGATTATAT GATTTCTATC ACCAGACTTC TTTTGGGACT GGACACCATA 
CCAGGATCGG GGTTTCTTTG TTCTATGAAA ATTACTGAAG ATGACTTGTG GATCAGAACG 
TATGCCAGAC TCTATCAGAA GCTGTGTTCT TCTACTGGAG ATGTTCCCAT TGGGATCTAC 
AGAACAGAAT CTCAGAAGCT AACTACATCT GAGTCTCGAG AAATAGCATC TCAATCTCAA 
ATATCCATCA GTGTGGAAGA GTGGGAAGAC ACCAAAGATG TCAAAGACCC AGGGCACCAC 
CGCAGCATTC ACCGCAACTC AACCTCTAGC GACCAGTCAG ACCACCCCTT GCTGCGGAGG 
AAGAGCATGC AGTGGGCCCG GAGGCTGAGC AGAAAAGGGC CAAAGCACTC TGGTAAAACT 
GCAGAAAAGA TAACCCAGCA ACGACTGAAT CTCTACAGGA GGTCGGAAAG ACAGGAGCTT 
GCTGAACTTG TGAAAAACAG AATGAAACAC CTGGGCCTCT CTACGGTGGG CTACGATGAA 
ATGAATGATC ATCAGAGCAC CCTTTCCTAC ATCCTGATTA ATCCATCTCC AGACACCAGG 
CTTGAGCTGA ATGATGTTGT ATATTTGATC CGACCAGATC CACTGTCCTA CCTTCCGAAC 
AGTGAACCCA GTAGGAAAAA CAGCATCTGC AATGCCGCTG TTCAAGATTC TCGGGAGGAA 
ACACAACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454292.1
CDS	579..4010
Translation	MVDLESEVPPLPPRYRFRDLLLGDQGWQNDDRVQVEFYMNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLSCLLYIIRVLLEKPSQGNDWSHIFWVNRSLPLWGLQVSVALISLFETILLGYLSYKGNIWEQILRVPFILEIINAVPFIISIFWPTLRNLFVPVFLNCWLAKHALENMINDLHRAIQRTQSAMFNQVLILISTLLCLIFTCFTDKTREHMYHVTLGFTETLTFSQSICGIQHLERIGKKLNLFDSLYFCIVTFSTVGFGDVTPETWSSKLFVVAMICVALVVLPIQFEQLAYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDVQVRRVLQIPMWSQRVIYLQGSALKDQDLLRAKMDNAEACFILSSRCEVDRTSSDHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCICPATSTLITLLVHTSRGQEGQQSPEQWQKTYGRCSGNEVYHIVLEESTFFAEYEGKSFTYASFHAHKKFGVCLVGVRREDNKNILLNPGPRYIMNASDICFYINITKEENSAFKNQDQQRKSNVSRSFYHGPSRLPVHSIIASMGTVAIDLQDTSCRAASGPTLALPSEGGKELRRPSIAPVLEVADTSSIQTCDLLSDQSEDETTPDEETSSNLEYAKGYPPYSPYIGSSPTFCHLLQEKVPFCCLRLDKSCQHNYYEDAKAYGFKNKLIIVAAETAGNGLYNFIVPLRAYYRPKKELNPIVLLLDNPLDDLLRCGVTFAANMVVVDKESTMSAEEDYMADAKTIVNVQTLFRLFSSLSIITELTHPANMRFMQFRAKDCYSLALSKLEKKERERGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMISITRLLLGLDTIPGSGFLCSMKITEDDLWIRTYARLYQKLCSSTGDVPIGIYRTESQKLTTSESREIASQSQISISVEEWEDTKDVKDPGHHRSIHRNSTSSDQSDHPLLRRKSMQWARRLSRKGPKHSGKTAEKITQQRLNLYRRSERQELAELVKNRMKHLGLSTVGYDEMNDHQSTLSYILINPSPDTRLELNDVVYLIRPDPLSYLPNSEPSRKNSICNAAVQDSREETQL

Target ORF information:

RefSeq Version	XM_017598803.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598803.1

ORF Insert Sequence:

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGTT TTACAGACAA AACAAGAGAG 
CACATGTATC ATGTGACTTT GGGATTTACT GAAACTTTGA CATTTTCTCA AAGCATTTGT 
GGAATTCAGC ATCTGGAACG AATTGGAAAG AAACTCAATC TCTTTGACTC CCTTTATTTC 
TGCATTGTGA CATTTTCTAC TGTGGGCTTT GGGGATGTTA CTCCTGAAAC ATGGTCCTCC 
AAGCTTTTTG TTGTTGCTAT GATCTGCGTT GCTCTTGTAG TTCTCCCCAT ACAGTTTGAA 
CAGCTGGCCT ATTTGTGGAT GGAGAGACAA AAGTCGGGAG GAAACTATAG TCGACACAGA 
GCTCAGACTG AGAAGCATGT TGTTCTGTGT GTCAGCTCAT TGAAGATTGA CTTGCTTATG 
GATTTTTTGA ATGAATTCTA TGCACATCCA AGACTGCAGG ATTATTATGT GGTGATTTTG 
TGTCCCACGG AGATGGATGT GCAAGTTCGG AGAGTGCTAC AGATTCCAAT GTGGTCCCAG 
AGAGTGATCT ACCTTCAAGG CTCTGCCCTT AAAGACCAGG ATCTCCTGAG AGCAAAGATG 
GACAATGCTG AGGCCTGTTT CATTTTGAGT AGCCGCTGTG AAGTCGATAG AACATCATCT 
GATCACCAGA CAATTTTGAG AGCATGGGCT GTAAAAGATT TTGCCCCAAA TTGTCCTCTG 
TATGTTCAGA TATTAAAACC AGAAAATAAA TTCCACATCA AATTTGCAGA TCATGTTGTT 
TGTGAAGAAG AGTTTAAGTA CGCCATGTTA GCTTTAAACT GTATATGCCC AGCAACATCT 
ACACTTATTA CACTACTGGT TCATACCTCT AGAGGGCAAG AAGGGCAGCA GTCACCTGAA 
CAGTGGCAGA AGACGTATGG GAGGTGCTCA GGAAATGAAG TCTACCACAT CGTTCTGGAA 
GAAAGTACAT TTTTTGCTGA ATATGAAGGG AAGAGTTTTA CCTATGCTTC TTTCCATGCA 
CACAAAAAGT TTGGTGTCTG CTTGGTTGGT GTTAGGAGGG AGGATAATAA AAACATTTTG 
CTGAATCCAG GTCCTCGATA CATTATGAAT GCTTCAGACA TATGTTTTTA TATTAATATT 
ACCAAAGAAG AAAATTCAGC ATTTAAGAAT CAAGACCAAC AGAGAAAAAG TAATGTGTCA 
AGGTCATTTT ATCATGGGCC TTCCAGATTG CCTGTCCACA GTATCATTGC CAGCATGGGT 
ACTGTGGCTA TAGACTTACA AGACACAAGC TGTAGAGCAG CAAGTGGCCC TACACTGGCT 
CTTCCTTCAG AGGGAGGCAA GGAACTAAGA AGACCTAGCA TTGCTCCTGT TTTAGAGGTA 
GCAGATACAT CATCAATTCA AACCTGCGAT CTTCTAAGTG ACCAGTCAGA AGATGAAACT 
ACACCAGATG AAGAAACATC TTCAAATTTA GAGTATGCCA AAGGCTACCC ACCTTATTCT 
CCATACATAG GAAGTTCACC TACTTTTTGT CATCTACTTC AAGAAAAAGT GCCCTTTTGC 
TGCTTAAGAT TAGACAAGAG TTGCCAGCAT AACTACTATG AGGATGCAAA AGCCTATGGA 
TTCAAAAATA AACTAATTAT AGTTGCAGCT GAAACGGCTG GGAACGGATT ATATAATTTT 
ATTGTACCTC TCAGGGCATA TTATAGACCA AAGAAAGAAT TAAATCCCAT AGTTCTTCTA 
TTGGATAATC CCCTAGATGA TTTGCTCAGG TGTGGAGTGA CCTTCGCTGC CAACATGGTG 
GTTGTGGACA AAGAAAGCAC CATGAGTGCA GAGGAAGACT ACATGGCAGA TGCCAAGACG 
ATTGTGAATG TGCAGACTCT TTTCAGGTTG TTTTCGAGTC TCAGTATTAT CACTGAGCTT 
ACTCATCCAG CAAATATGAG ATTCATGCAA TTCAGAGCCA AGGACTGTTA CTCTCTTGCA 
CTTTCAAAAC TGGAAAAGAA AGAGCGAGAA CGAGGTTCTA ACCTGGCCTT CATGTTCCGA 
CTGCCTTTCG CTGCGGGGAG AGTGTTCAGC ATCAGTATGC TGGACACGCT GCTTTATCAG 
TCATTTGTGA AAGATTATAT GATTTCTATC ACCAGACTTC TTTTGGGACT GGACACCATA 
CCAGGATCGG GGTTTCTTTG TTCTATGAAA ATTACTGAAG ATGACTTGTG GATCAGAACG 
TATGCCAGAC TCTATCAGAA GCTGTGTTCT TCTACTGGAG ATGTTCCCAT TGGGATCTAC 
AGAACAGAAT CTCAGAAGCT AACTACATCT GAGTCTCGAG AAATAGCATC TCAATCTCAA 
ATATCCATCA GTGTGGAAGA GTGGGAAGAC ACCAAAGATG TCAAAGACCC AGGGCACCAC 
CGCAGCATTC ACCGCAACTC AACCTCTAGC GACCAGTCAG ACCACCCCTT GCTGCGGAGG 
AAGAGCATGC AGTGGGCCCG GAGGCTGAGC AGAAAAGGGC CAAAGCACTC TGGTAAAACT 
GCAGAAAAGA TAACCCAGCA ACGACTGAAT CTCTACAGGA GGTCGGAAAG ACAGGAGCTT 
GCTGAACTTG TGAAAAACAG AATGAAACAC CTGGGCCTCT CTACGGTGGG CTACGATGAA 
ATGAATGATC ATCAGAGCAC CCTTTCCTAC ATCCTGATTA ATCCATCTCC AGACACCAGG 
CTTGAGCTGA ATGATGTTGT ATATTTGATC CGACCAGATC CACTGTCCTA CCTTCCGAAC 
AGTGAACCCA GTAGGAAAAA CAGCATCTGC AATGCCGCTG TTCAAGATTC TCGGGAGGAA 
ACACAACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt2 ( XM_017598804.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454293.1 Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa58069
Clone ID Related Accession (Same CDS sequence)	XM_017598804.1
Accession Version	XM_017598804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium channel subfamily T member 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGCA TTTGTGGAAT TCAGCATCTG 
GAACGAATTG GAAAGAAACT CAATCTCTTT GACTCCCTTT ATTTCTGCAT TGTGACATTT 
TCTACTGTGG GCTTTGGGGA TGTTACTCCT GAAACATGGT CCTCCAAGCT TTTTGTTGTT 
GCTATGATCT GCGTTGCTCT TGTAGTTCTC CCCATACAGT TTGAACAGCT GGCCTATTTG 
TGGATGGAGA GACAAAAGTC GGGAGGAAAC TATAGTCGAC ACAGAGCTCA GACTGAGAAG 
CATGTTGTTC TGTGTGTCAG CTCATTGAAG ATTGACTTGC TTATGGATTT TTTGAATGAA 
TTCTATGCAC ATCCAAGACT GCAGGATTAT TATGTGGTGA TTTTGTGTCC CACGGAGATG 
GATGTGCAAG TTCGGAGAGT GCTACAGATT CCAATGTGGT CCCAGAGAGT GATCTACCTT 
CAAGGCTCTG CCCTTAAAGA CCAGGATCTC CTGAGAGCAA AGATGGACAA TGCTGAGGCC 
TGTTTCATTT TGAGTAGCCG CTGTGAAGTC GATAGAACAT CATCTGATCA CCAGACAATT 
TTGAGAGCAT GGGCTGTAAA AGATTTTGCC CCAAATTGTC CTCTGTATGT TCAGATATTA 
AAACCAGAAA ATAAATTCCA CATCAAATTT GCAGATCATG TTGTTTGTGA AGAAGAGTTT 
AAGTACGCCA TGTTAGCTTT AAACTGTATA TGCCCAGCAA CATCTACACT TATTACACTA 
CTGGTTCATA CCTCTAGAGG GCAAGAAGGG CAGCAGTCAC CTGAACAGTG GCAGAAGACG 
TATGGGAGGT GCTCAGGAAA TGAAGTCTAC CACATCGTTC TGGAAGAAAG TACATTTTTT 
GCTGAATATG AAGGGAAGAG TTTTACCTAT GCTTCTTTCC ATGCACACAA AAAGTTTGGT 
GTCTGCTTGG TTGGTGTTAG GAGGGAGGAT AATAAAAACA TTTTGCTGAA TCCAGGTCCT 
CGATACATTA TGAATGCTTC AGACATATGT TTTTATATTA ATATTACCAA AGAAGAAAAT 
TCAGCATTTA AGAATCAAGA CCAACAGAGA AAAAGTAATG TGTCAAGGTC ATTTTATCAT 
GGGCCTTCCA GATTGCCTGT CCACAGTATC ATTGCCAGCA TGGGTACTGT GGCTATAGAC 
TTACAAGACA CAAGCTGTAG AGCAGCAAGT GGCCCTACAC TGGCTCTTCC TTCAGAGGGA 
GGCAAGGAAC TAAGAAGACC TAGCATTGCT CCTGTTTTAG AGGTAGCAGA TACATCATCA 
ATTCAAACCT GCGATCTTCT AAGTGACCAG TCAGAAGATG AAACTACACC AGATGAAGAA 
ACATCTTCAA ATTTAGAGTA TGCCAAAGGC TACCCACCTT ATTCTCCATA CATAGGAAGT 
TCACCTACTT TTTGTCATCT ACTTCAAGAA AAAGTGCCCT TTTGCTGCTT AAGATTAGAC 
AAGAGTTGCC AGCATAACTA CTATGAGGAT GCAAAAGCCT ATGGATTCAA AAATAAACTA 
ATTATAGTTG CAGCTGAAAC GGCTGGGAAC GGATTATATA ATTTTATTGT ACCTCTCAGG 
GCATATTATA GACCAAAGAA AGAATTAAAT CCCATAGTTC TTCTATTGGA TAATCCGCCA 
GATATGCATT TTCTGGATGC AATCTGTTGG TTTCCAATGG TTTACTACAT GGTGGGCTCT 
ATTGACAACC TAGATGATTT GCTCAGGTGT GGAGTGACCT TCGCTGCCAA CATGGTGGTT 
GTGGACAAAG AAAGCACCAT GAGTGCAGAG GAAGACTACA TGGCAGATGC CAAGACGATT 
GTGAATGTGC AGACTCTTTT CAGGTTGTTT TCGAGTCTCA GTATTATCAC TGAGCTTACT 
CATCCAGCAA ATATGAGATT CATGCAATTC AGAGCCAAGG ACTGTTACTC TCTTGCACTT 
TCAAAACTGG AAAAGAAAGA GCGAGAACGA GGTTCTAACC TGGCCTTCAT GTTCCGACTG 
CCTTTCGCTG CGGGGAGAGT GTTCAGCATC AGTATGCTGG ACACGCTGCT TTATCAGTCA 
TTTGTGAAAG ATTATATGAT TTCTATCACC AGACTTCTTT TGGGACTGGA CACCATACCA 
GGATCGGGGT TTCTTTGTTC TATGAAAATT ACTGAAGATG ACTTGTGGAT CAGAACGTAT 
GCCAGACTCT ATCAGAAGCT GTGTTCTTCT ACTGGAGATG TTCCCATTGG GATCTACAGA 
ACAGAATCTC AGAAGCTAAC TACATCTGAG TCTCGAGAAA TAGCATCTCA ATCTCAAATA 
TCCATCAGTG TGGAAGAGTG GGAAGACACC AAAGATGTCA AAGACCCAGG GCACCACCGC 
AGCATTCACC GCAACTCAAC CTCTAGCGAC CAGTCAGACC ACCCCTTGCT GCGGAGGAAG 
AGCATGCAGT GGGCCCGGAG GCTGAGCAGA AAAGGGCCAA AGCACTCTGG TAAAACTGCA 
GAAAAGATAA CCCAGCAACG ACTGAATCTC TACAGGAGGT CGGAAAGACA GGAGCTTGCT 
GAACTTGTGA AAAACAGAAT GAAACACCTG GGCCTCTCTA CGGTGGGCTA CGATGAAATG 
AATGATCATC AGAGCACCCT TTCCTACATC CTGATTAATC CATCTCCAGA CACCAGGCTT 
GAGCTGAATG ATGTTGTATA TTTGATCCGA CCAGATCCAC TGTCCTACCT TCCGAACAGT 
GAACCCAGTA GGAAAAACAG CATCTGCAAT GCCGCTGTTC AAGATTCTCG GGAGGAAACA 
CAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454293.1
CDS	579..4007
Translation	MVDLESEVPPLPPRYRFRDLLLGDQGWQNDDRVQVEFYMNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLSCLLYIIRVLLEKPSQGNDWSHIFWVNRSLPLWGLQVSVALISLFETILLGYLSYKGNIWEQILRVPFILEIINAVPFIISIFWPTLRNLFVPVFLNCWLAKHALENMINDLHRAIQRTQSAMFNQVLILISTLLCLIFTCICGIQHLERIGKKLNLFDSLYFCIVTFSTVGFGDVTPETWSSKLFVVAMICVALVVLPIQFEQLAYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDVQVRRVLQIPMWSQRVIYLQGSALKDQDLLRAKMDNAEACFILSSRCEVDRTSSDHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCICPATSTLITLLVHTSRGQEGQQSPEQWQKTYGRCSGNEVYHIVLEESTFFAEYEGKSFTYASFHAHKKFGVCLVGVRREDNKNILLNPGPRYIMNASDICFYINITKEENSAFKNQDQQRKSNVSRSFYHGPSRLPVHSIIASMGTVAIDLQDTSCRAASGPTLALPSEGGKELRRPSIAPVLEVADTSSIQTCDLLSDQSEDETTPDEETSSNLEYAKGYPPYSPYIGSSPTFCHLLQEKVPFCCLRLDKSCQHNYYEDAKAYGFKNKLIIVAAETAGNGLYNFIVPLRAYYRPKKELNPIVLLLDNPPDMHFLDAICWFPMVYYMVGSIDNLDDLLRCGVTFAANMVVVDKESTMSAEEDYMADAKTIVNVQTLFRLFSSLSIITELTHPANMRFMQFRAKDCYSLALSKLEKKERERGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMISITRLLLGLDTIPGSGFLCSMKITEDDLWIRTYARLYQKLCSSTGDVPIGIYRTESQKLTTSESREIASQSQISISVEEWEDTKDVKDPGHHRSIHRNSTSSDQSDHPLLRRKSMQWARRLSRKGPKHSGKTAEKITQQRLNLYRRSERQELAELVKNRMKHLGLSTVGYDEMNDHQSTLSYILINPSPDTRLELNDVVYLIRPDPLSYLPNSEPSRKNSICNAAVQDSREETQL

Target ORF information:

RefSeq Version	XM_017598804.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598804.1

ORF Insert Sequence:

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGCA TTTGTGGAAT TCAGCATCTG 
GAACGAATTG GAAAGAAACT CAATCTCTTT GACTCCCTTT ATTTCTGCAT TGTGACATTT 
TCTACTGTGG GCTTTGGGGA TGTTACTCCT GAAACATGGT CCTCCAAGCT TTTTGTTGTT 
GCTATGATCT GCGTTGCTCT TGTAGTTCTC CCCATACAGT TTGAACAGCT GGCCTATTTG 
TGGATGGAGA GACAAAAGTC GGGAGGAAAC TATAGTCGAC ACAGAGCTCA GACTGAGAAG 
CATGTTGTTC TGTGTGTCAG CTCATTGAAG ATTGACTTGC TTATGGATTT TTTGAATGAA 
TTCTATGCAC ATCCAAGACT GCAGGATTAT TATGTGGTGA TTTTGTGTCC CACGGAGATG 
GATGTGCAAG TTCGGAGAGT GCTACAGATT CCAATGTGGT CCCAGAGAGT GATCTACCTT 
CAAGGCTCTG CCCTTAAAGA CCAGGATCTC CTGAGAGCAA AGATGGACAA TGCTGAGGCC 
TGTTTCATTT TGAGTAGCCG CTGTGAAGTC GATAGAACAT CATCTGATCA CCAGACAATT 
TTGAGAGCAT GGGCTGTAAA AGATTTTGCC CCAAATTGTC CTCTGTATGT TCAGATATTA 
AAACCAGAAA ATAAATTCCA CATCAAATTT GCAGATCATG TTGTTTGTGA AGAAGAGTTT 
AAGTACGCCA TGTTAGCTTT AAACTGTATA TGCCCAGCAA CATCTACACT TATTACACTA 
CTGGTTCATA CCTCTAGAGG GCAAGAAGGG CAGCAGTCAC CTGAACAGTG GCAGAAGACG 
TATGGGAGGT GCTCAGGAAA TGAAGTCTAC CACATCGTTC TGGAAGAAAG TACATTTTTT 
GCTGAATATG AAGGGAAGAG TTTTACCTAT GCTTCTTTCC ATGCACACAA AAAGTTTGGT 
GTCTGCTTGG TTGGTGTTAG GAGGGAGGAT AATAAAAACA TTTTGCTGAA TCCAGGTCCT 
CGATACATTA TGAATGCTTC AGACATATGT TTTTATATTA ATATTACCAA AGAAGAAAAT 
TCAGCATTTA AGAATCAAGA CCAACAGAGA AAAAGTAATG TGTCAAGGTC ATTTTATCAT 
GGGCCTTCCA GATTGCCTGT CCACAGTATC ATTGCCAGCA TGGGTACTGT GGCTATAGAC 
TTACAAGACA CAAGCTGTAG AGCAGCAAGT GGCCCTACAC TGGCTCTTCC TTCAGAGGGA 
GGCAAGGAAC TAAGAAGACC TAGCATTGCT CCTGTTTTAG AGGTAGCAGA TACATCATCA 
ATTCAAACCT GCGATCTTCT AAGTGACCAG TCAGAAGATG AAACTACACC AGATGAAGAA 
ACATCTTCAA ATTTAGAGTA TGCCAAAGGC TACCCACCTT ATTCTCCATA CATAGGAAGT 
TCACCTACTT TTTGTCATCT ACTTCAAGAA AAAGTGCCCT TTTGCTGCTT AAGATTAGAC 
AAGAGTTGCC AGCATAACTA CTATGAGGAT GCAAAAGCCT ATGGATTCAA AAATAAACTA 
ATTATAGTTG CAGCTGAAAC GGCTGGGAAC GGATTATATA ATTTTATTGT ACCTCTCAGG 
GCATATTATA GACCAAAGAA AGAATTAAAT CCCATAGTTC TTCTATTGGA TAATCCGCCA 
GATATGCATT TTCTGGATGC AATCTGTTGG TTTCCAATGG TTTACTACAT GGTGGGCTCT 
ATTGACAACC TAGATGATTT GCTCAGGTGT GGAGTGACCT TCGCTGCCAA CATGGTGGTT 
GTGGACAAAG AAAGCACCAT GAGTGCAGAG GAAGACTACA TGGCAGATGC CAAGACGATT 
GTGAATGTGC AGACTCTTTT CAGGTTGTTT TCGAGTCTCA GTATTATCAC TGAGCTTACT 
CATCCAGCAA ATATGAGATT CATGCAATTC AGAGCCAAGG ACTGTTACTC TCTTGCACTT 
TCAAAACTGG AAAAGAAAGA GCGAGAACGA GGTTCTAACC TGGCCTTCAT GTTCCGACTG 
CCTTTCGCTG CGGGGAGAGT GTTCAGCATC AGTATGCTGG ACACGCTGCT TTATCAGTCA 
TTTGTGAAAG ATTATATGAT TTCTATCACC AGACTTCTTT TGGGACTGGA CACCATACCA 
GGATCGGGGT TTCTTTGTTC TATGAAAATT ACTGAAGATG ACTTGTGGAT CAGAACGTAT 
GCCAGACTCT ATCAGAAGCT GTGTTCTTCT ACTGGAGATG TTCCCATTGG GATCTACAGA 
ACAGAATCTC AGAAGCTAAC TACATCTGAG TCTCGAGAAA TAGCATCTCA ATCTCAAATA 
TCCATCAGTG TGGAAGAGTG GGAAGACACC AAAGATGTCA AAGACCCAGG GCACCACCGC 
AGCATTCACC GCAACTCAAC CTCTAGCGAC CAGTCAGACC ACCCCTTGCT GCGGAGGAAG 
AGCATGCAGT GGGCCCGGAG GCTGAGCAGA AAAGGGCCAA AGCACTCTGG TAAAACTGCA 
GAAAAGATAA CCCAGCAACG ACTGAATCTC TACAGGAGGT CGGAAAGACA GGAGCTTGCT 
GAACTTGTGA AAAACAGAAT GAAACACCTG GGCCTCTCTA CGGTGGGCTA CGATGAAATG 
AATGATCATC AGAGCACCCT TTCCTACATC CTGATTAATC CATCTCCAGA CACCAGGCTT 
GAGCTGAATG ATGTTGTATA TTTGATCCGA CCAGATCCAC TGTCCTACCT TCCGAACAGT 
GAACCCAGTA GGAAAAACAG CATCTGCAAT GCCGCTGTTC AAGATTCTCG GGAGGAAACA 
CAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt2 ( XM_017598806.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454295.1 Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa58071
Clone ID Related Accession (Same CDS sequence)	XM_017598806.1
Accession Version	XM_017598806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium channel subfamily T member 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGCA TTTGTGGAAT TCAGCATCTG 
GAACGAATTG GAAAGAAACT CAATCTCTTT GACTCCCTTT ATTTCTGCAT TGTGACATTT 
TCTACTGTGG GCTTTGGGGA TGTTACTCCT GAAACATGGT CCTCCAAGCT TTTTGTTGTT 
GCTATGATCT GCGTTGCTCT TGTAGTTCTC CCCATACAGT TTGAACAGCT GGCCTATTTG 
TGGATGGAGA GACAAAAGTC GGGAGGAAAC TATAGTCGAC ACAGAGCTCA GACTGAGAAG 
CATGTTGTTC TGTGTGTCAG CTCATTGAAG ATTGACTTGC TTATGGATTT TTTGAATGAA 
TTCTATGCAC ATCCAAGACT GCAGGATTAT TATGTGGTGA TTTTGTGTCC CACGGAGATG 
GATGTGCAAG TTCGGAGAGT GCTACAGATT CCAATGTGGT CCCAGAGAGT GATCTACCTT 
CAAGGCTCTG CCCTTAAAGA CCAGGATCTC CTGAGAGCAA AGATGGACAA TGCTGAGGCC 
TGTTTCATTT TGAGTAGCCG CTGTGAAGTC GATAGAACAT CATCTGATCA CCAGACAATT 
TTGAGAGCAT GGGCTGTAAA AGATTTTGCC CCAAATTGTC CTCTGTATGT TCAGATATTA 
AAACCAGAAA ATAAATTCCA CATCAAATTT GCAGATCATG TTGTTTGTGA AGAAGAGTTT 
AAGTACGCCA TGTTAGCTTT AAACTGTATA TGCCCAGCAA CATCTACACT TATTACACTA 
CTGGTTCATA CCTCTAGAGG GCAGTTTGGT GTCTGCTTGG TTGGTGTTAG GAGGGAGGAT 
AATAAAAACA TTTTGCTGAA TCCAGGTCCT CGATACATTA TGAATGCTTC AGACATATGT 
TTTTATATTA ATATTACCAA AGAAGAAAAT TCAGCATTTA AGAATCAAGA CCAACAGAGA 
AAAAGTAATG TGTCAAGGTC ATTTTATCAT GGGCCTTCCA GATTGCCTGT CCACAGTATC 
ATTGCCAGCA TGGGTACTGT GGCTATAGAC TTACAAGACA CAAGCTGTAG AGCAGCAAGT 
GGCCCTACAC TGGCTCTTCC TTCAGAGGGA GGCAAGGAAC TAAGAAGACC TAGCATTGCT 
CCTGTTTTAG AGGTAGCAGA TACATCATCA ATTCAAACCT GCGATCTTCT AAGTGACCAG 
TCAGAAGATG AAACTACACC AGATGAAGAA ACATCTTCAA ATTTAGAGTA TGCCAAAGGC 
TACCCACCTT ATTCTCCATA CATAGGAAGT TCACCTACTT TTTGTCATCT ACTTCAAGAA 
AAAGTGCCCT TTTGCTGCTT AAGATTAGAC AAGAGTTGCC AGCATAACTA CTATGAGGAT 
GCAAAAGCCT ATGGATTCAA AAATAAACTA ATTATAGTTG CAGCTGAAAC GGCTGGGAAC 
GGATTATATA ATTTTATTGT ACCTCTCAGG GCATATTATA GACCAAAGAA AGAATTAAAT 
CCCATAGTTC TTCTATTGGA TAATCCCCTA GATGATTTGC TCAGGTGTGG AGTGACCTTC 
GCTGCCAACA TGGTGGTTGT GGACAAAGAA AGCACCATGA GTGCAGAGGA AGACTACATG 
GCAGATGCCA AGACGATTGT GAATGTGCAG ACTCTTTTCA GGTTGTTTTC GAGTCTCAGT 
ATTATCACTG AGCTTACTCA TCCAGCAAAT ATGAGATTCA TGCAATTCAG AGCCAAGGAC 
TGTTACTCTC TTGCACTTTC AAAACTGGAA AAGAAAGAGC GAGAACGAGG TTCTAACCTG 
GCCTTCATGT TCCGACTGCC TTTCGCTGCG GGGAGAGTGT TCAGCATCAG TATGCTGGAC 
ACGCTGCTTT ATCAGTCATT TGTGAAAGAT TATATGATTT CTATCACCAG ACTTCTTTTG 
GGACTGGACA CCATACCAGG ATCGGGGTTT CTTTGTTCTA TGAAAATTAC TGAAGATGAC 
TTGTGGATCA GAACGTATGC CAGACTCTAT CAGAAGCTGT GTTCTTCTAC TGGAGATGTT 
CCCATTGGGA TCTACAGAAC AGAATCTCAG AAGCTAACTA CATCTGAGTC TCGAGAAATA 
GCATCTCAAT CTCAAATATC CATCAGTGTG GAAGAGTGGG AAGACACCAA AGATGTCAAA 
GACCCAGGGC ACCACCGCAG CATTCACCGC AACTCAACCT CTAGCGACCA GTCAGACCAC 
CCCTTGCTGC GGAGGAAGAG CATGCAGTGG GCCCGGAGGC TGAGCAGAAA AGGGCCAAAG 
CACTCTGGTA AAACTGCAGA AAAGATAACC CAGCAACGAC TGAATCTCTA CAGGAGGTCG 
GAAAGACAGG AGCTTGCTGA ACTTGTGAAA AACAGAATGA AACACCTGGG CCTCTCTACG 
GTGGGCTACG ATGAAATGAA TGATCATCAG AGCACCCTTT CCTACATCCT GATTAATCCA 
TCTCCAGACA CCAGGCTTGA GCTGAATGAT GTTGTATATT TGATCCGACC AGATCCACTG 
TCCTACCTTC CGAACAGTGA ACCCAGTAGG AAAAACAGCA TCTGCAATGC CGCTGTTCAA 
GATTCTCGGG AGGAAACACA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454295.1
CDS	579..3785
Translation	MVDLESEVPPLPPRYRFRDLLLGDQGWQNDDRVQVEFYMNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLSCLLYIIRVLLEKPSQGNDWSHIFWVNRSLPLWGLQVSVALISLFETILLGYLSYKGNIWEQILRVPFILEIINAVPFIISIFWPTLRNLFVPVFLNCWLAKHALENMINDLHRAIQRTQSAMFNQVLILISTLLCLIFTCICGIQHLERIGKKLNLFDSLYFCIVTFSTVGFGDVTPETWSSKLFVVAMICVALVVLPIQFEQLAYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDVQVRRVLQIPMWSQRVIYLQGSALKDQDLLRAKMDNAEACFILSSRCEVDRTSSDHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCICPATSTLITLLVHTSRGQFGVCLVGVRREDNKNILLNPGPRYIMNASDICFYINITKEENSAFKNQDQQRKSNVSRSFYHGPSRLPVHSIIASMGTVAIDLQDTSCRAASGPTLALPSEGGKELRRPSIAPVLEVADTSSIQTCDLLSDQSEDETTPDEETSSNLEYAKGYPPYSPYIGSSPTFCHLLQEKVPFCCLRLDKSCQHNYYEDAKAYGFKNKLIIVAAETAGNGLYNFIVPLRAYYRPKKELNPIVLLLDNPLDDLLRCGVTFAANMVVVDKESTMSAEEDYMADAKTIVNVQTLFRLFSSLSIITELTHPANMRFMQFRAKDCYSLALSKLEKKERERGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMISITRLLLGLDTIPGSGFLCSMKITEDDLWIRTYARLYQKLCSSTGDVPIGIYRTESQKLTTSESREIASQSQISISVEEWEDTKDVKDPGHHRSIHRNSTSSDQSDHPLLRRKSMQWARRLSRKGPKHSGKTAEKITQQRLNLYRRSERQELAELVKNRMKHLGLSTVGYDEMNDHQSTLSYILINPSPDTRLELNDVVYLIRPDPLSYLPNSEPSRKNSICNAAVQDSREETQL

Target ORF information:

RefSeq Version	XM_017598806.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598806.1

ORF Insert Sequence:

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGCA TTTGTGGAAT TCAGCATCTG 
GAACGAATTG GAAAGAAACT CAATCTCTTT GACTCCCTTT ATTTCTGCAT TGTGACATTT 
TCTACTGTGG GCTTTGGGGA TGTTACTCCT GAAACATGGT CCTCCAAGCT TTTTGTTGTT 
GCTATGATCT GCGTTGCTCT TGTAGTTCTC CCCATACAGT TTGAACAGCT GGCCTATTTG 
TGGATGGAGA GACAAAAGTC GGGAGGAAAC TATAGTCGAC ACAGAGCTCA GACTGAGAAG 
CATGTTGTTC TGTGTGTCAG CTCATTGAAG ATTGACTTGC TTATGGATTT TTTGAATGAA 
TTCTATGCAC ATCCAAGACT GCAGGATTAT TATGTGGTGA TTTTGTGTCC CACGGAGATG 
GATGTGCAAG TTCGGAGAGT GCTACAGATT CCAATGTGGT CCCAGAGAGT GATCTACCTT 
CAAGGCTCTG CCCTTAAAGA CCAGGATCTC CTGAGAGCAA AGATGGACAA TGCTGAGGCC 
TGTTTCATTT TGAGTAGCCG CTGTGAAGTC GATAGAACAT CATCTGATCA CCAGACAATT 
TTGAGAGCAT GGGCTGTAAA AGATTTTGCC CCAAATTGTC CTCTGTATGT TCAGATATTA 
AAACCAGAAA ATAAATTCCA CATCAAATTT GCAGATCATG TTGTTTGTGA AGAAGAGTTT 
AAGTACGCCA TGTTAGCTTT AAACTGTATA TGCCCAGCAA CATCTACACT TATTACACTA 
CTGGTTCATA CCTCTAGAGG GCAGTTTGGT GTCTGCTTGG TTGGTGTTAG GAGGGAGGAT 
AATAAAAACA TTTTGCTGAA TCCAGGTCCT CGATACATTA TGAATGCTTC AGACATATGT 
TTTTATATTA ATATTACCAA AGAAGAAAAT TCAGCATTTA AGAATCAAGA CCAACAGAGA 
AAAAGTAATG TGTCAAGGTC ATTTTATCAT GGGCCTTCCA GATTGCCTGT CCACAGTATC 
ATTGCCAGCA TGGGTACTGT GGCTATAGAC TTACAAGACA CAAGCTGTAG AGCAGCAAGT 
GGCCCTACAC TGGCTCTTCC TTCAGAGGGA GGCAAGGAAC TAAGAAGACC TAGCATTGCT 
CCTGTTTTAG AGGTAGCAGA TACATCATCA ATTCAAACCT GCGATCTTCT AAGTGACCAG 
TCAGAAGATG AAACTACACC AGATGAAGAA ACATCTTCAA ATTTAGAGTA TGCCAAAGGC 
TACCCACCTT ATTCTCCATA CATAGGAAGT TCACCTACTT TTTGTCATCT ACTTCAAGAA 
AAAGTGCCCT TTTGCTGCTT AAGATTAGAC AAGAGTTGCC AGCATAACTA CTATGAGGAT 
GCAAAAGCCT ATGGATTCAA AAATAAACTA ATTATAGTTG CAGCTGAAAC GGCTGGGAAC 
GGATTATATA ATTTTATTGT ACCTCTCAGG GCATATTATA GACCAAAGAA AGAATTAAAT 
CCCATAGTTC TTCTATTGGA TAATCCCCTA GATGATTTGC TCAGGTGTGG AGTGACCTTC 
GCTGCCAACA TGGTGGTTGT GGACAAAGAA AGCACCATGA GTGCAGAGGA AGACTACATG 
GCAGATGCCA AGACGATTGT GAATGTGCAG ACTCTTTTCA GGTTGTTTTC GAGTCTCAGT 
ATTATCACTG AGCTTACTCA TCCAGCAAAT ATGAGATTCA TGCAATTCAG AGCCAAGGAC 
TGTTACTCTC TTGCACTTTC AAAACTGGAA AAGAAAGAGC GAGAACGAGG TTCTAACCTG 
GCCTTCATGT TCCGACTGCC TTTCGCTGCG GGGAGAGTGT TCAGCATCAG TATGCTGGAC 
ACGCTGCTTT ATCAGTCATT TGTGAAAGAT TATATGATTT CTATCACCAG ACTTCTTTTG 
GGACTGGACA CCATACCAGG ATCGGGGTTT CTTTGTTCTA TGAAAATTAC TGAAGATGAC 
TTGTGGATCA GAACGTATGC CAGACTCTAT CAGAAGCTGT GTTCTTCTAC TGGAGATGTT 
CCCATTGGGA TCTACAGAAC AGAATCTCAG AAGCTAACTA CATCTGAGTC TCGAGAAATA 
GCATCTCAAT CTCAAATATC CATCAGTGTG GAAGAGTGGG AAGACACCAA AGATGTCAAA 
GACCCAGGGC ACCACCGCAG CATTCACCGC AACTCAACCT CTAGCGACCA GTCAGACCAC 
CCCTTGCTGC GGAGGAAGAG CATGCAGTGG GCCCGGAGGC TGAGCAGAAA AGGGCCAAAG 
CACTCTGGTA AAACTGCAGA AAAGATAACC CAGCAACGAC TGAATCTCTA CAGGAGGTCG 
GAAAGACAGG AGCTTGCTGA ACTTGTGAAA AACAGAATGA AACACCTGGG CCTCTCTACG 
GTGGGCTACG ATGAAATGAA TGATCATCAG AGCACCCTTT CCTACATCCT GATTAATCCA 
TCTCCAGACA CCAGGCTTGA GCTGAATGAT GTTGTATATT TGATCCGACC AGATCCACTG 
TCCTACCTTC CGAACAGTGA ACCCAGTAGG AAAAACAGCA TCTGCAATGC CGCTGTTCAA 
GATTCTCGGG AGGAAACACA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt2 ( NM_198762.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_942057.1 Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), mRNA.

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CloneID	ORa04991
Clone ID Related Accession (Same CDS sequence)	NM_198762.1
Accession Version	NM_198762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium channel subfamily T member 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY359443.1. On Dec 17, 2003 this sequence version replaced XM_222699.2. ##Evidence-Data-START## Transcript exon combination :: AY359443.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGCA TTTGTGGAAT TCAGCATCTG 
GAACGAATTG GAAAGAAACT CAATCTCTTT GACTCCCTGT ATTTCTGCAT TGTGACATTT 
TCTACTGTGG GCTTTGGGGA TGTTACTCCT GAAACATGGT CCTCCAAGCT TTTTGTTGTT 
GCTATGATCT GCGTTGCTCT TGTAGTTCTC CCCATACAGT TTGAACAGCT GGCCTATTTG 
TGGATGGAGA GACAAAAGTC GGGAGGAAAC TATAGTCGAC ACAGAGCTCA GACTGAGAAG 
CATGTTGTTC TGTGTGTCAG CTCATTGAAG ATTGACTTGC TTATGGATTT TTTGAATGAA 
TTCTATGCAC ATCCAAGACT GCAGGATTAT TATGTGGTGA TTTTGTGTCC CACGGAGATG 
GATGTGCAAG TTCGGAGAGT GCTACAGATT CCAATGTGGT CCCAGAGAGT GATCTACCTT 
CAAGGCTCTG CCCTTAAAGA CCAGGATCTC CTGAGAGCAA AGATGGACAA TGCTGAGGCC 
TGTTTCATTT TGAGTAGCCG CTGTGAAGTC GATAGAACAT CATCTGATCA CCAGACAATT 
TTGAGAGCAT GGGCTGTAAA AGATTTTGCC CCAAATTGTC CTCTGTATGT TCAGATATTA 
AAACCAGAAA ATAAATTCCA CATCAAATTT GCAGATCATG TTGTTTGTGA AGAAGAGTTT 
AAGTACGCCA TGTTAGCTTT AAACTGTATA TGCCCAGCAA CATCTACACT TATTACACTA 
CTGGTTCATA CCTCTAGAGG GCAGTGTGTG TGCTTGTGTT GCAGAGAAGG GCAGCAGTCA 
CCTGAACAGT GGCAGAAGAC GTATGGGAGG TGCTCAGGAA ATGAAGTCTA CCACATCGTT 
CTGGAAGAAA GTACATTTTT TGCTGAATAT GAAGGGAAGA GTTTTACCTA TGCTTCTTTC 
CATGCACACA AAAAGTTTGG TGTCTGCTTG GTTGGTGTTA GGAGGGAGGA TAATAAAAAC 
ATTTTGCTGA ATCCAGGTCC TCGATACATT ATGAATGCTT CAGACATATG TTTTTATATT 
AATATTACCA AAGAAGAAAA TTCAGCATTT AAGAATCAAG ACCAACAGAG AAAAAGTAAT 
GTGTCAAGGT CATTTTATCA TGGGCCTTCC AGATTGCCTG TCCACAGTAT CATTGCCAGC 
ATGGGTACTG TGGCTATAGA CTTACAAGAC ACAAGCTGTA GAGCAGCAAG TGGCCCTACA 
CTGGCTCTTC CTTCAGAGGG AGGCAAGGAA CTAAGAAGAC CTAGCATTGC TCCTGTTTTA 
GAGGTAGCAG ATACATCATC AATTCAAACC TGCGATCTTC TAAGTGACCA GTCAGAAGAT 
GAAACTACAC CAGATGAAGA AACATCTTCA AATTTAGAGT ATGCCAAAGG CTACCCACCT 
TATTCTCCAT ACATAGGAAG TTCACCTACT TTTTGTCATC TACTTCAAGA AAAAGTGCCC 
TTTTGCTGCC TAAGATTAGA CAAGAGTTGC CAGCATAACT ACTATGAGGA TGCAAAAGCC 
TATGGATTCA AAAATAAACT AATTATAGTT GCAGCTGAAA CGGCTGGGAA CGGATTATAT 
AATTTTATTG TACCTCTCAG GGCATATTAT AGACCAAAGA AAGAATTAAA TCCCATAGTT 
CTTCTATTGG ATAATCCGCC AGATATGCAT TTTCTGGATG CAATCTGTTG GTTTCCAATG 
GTTTACTACA TGGTGGGCTC TATTGACAAC CTAGATGATT TGCTCAGGTG TGGAGTGACC 
TTCGCTGCCA ACATGGTGGT TGTGGACAAA GAAAGCACCA TGAGTGCAGA GGAAGACTAC 
ATGGCAGATG CCAAGACGAT TGTGAATGTG CAGACTCTTT TCAGGTTGTT TTCGAGTCTC 
AGTATTATCA CTGAGCTTAC TCATCCAGCA AATATGAGAT TCATGCAATT CAGAGCCAAG 
GACTGTTACT CTCTTGCACT TTCAAAACTG GAAAAGAAAG AGCGAGAACG AGGTTCTAAC 
CTGGCCTTCA TGTTCCGACT GCCTTTCGCT GCGGGGAGAG TGTTCAGCAT CAGTATGCTG 
GACACGCTGC TTTATCAGTC ATTTGTGAAA GATTATATGA TTTCTATCAC CAGACTTCTT 
TTGGGACTGG ACACCATACC AGGATCGGGG TTTCTTTGTT CTATGAAAAT TACTGAAGAT 
GACTTGTGGA TCAGAACGTA TGCCAGACTC TATCAGAAGC TGTGTTCTTC TACTGGAGAT 
GTTCCCATTG GGATCTACAG AACAGAATCT CAGAAGCTAA CTACATCTGA GTCTCAAATA 
TCCATCAGTG TGGAAGAGTG GGAAGACACC AAAGATGTCA AAGACCCAGG GCACCACCGC 
AGCATTCACC GCAACTCAAC CTCTAGCGAC CAGTCAGACC ACCCCTTGCT GCGGAGGAAG 
AGCATGCAGT GGGCCCGGAG GCTGAGCAGA AAAGGGCCAA AGCACTCTGG TAAAACTGCA 
GAAAAGATAA CCCAGCAACG ACTGAATCTC TACAGGAGGT CGGAAAGACA GGAGCTTGCT 
GAACTTGTGA AAAACAGAAT GAAACACCTG GGCCTCTCTA CGGTGGGCTA CGATGAAATG 
AATGATCATC AGAGCACCCT TTCCTACATC CTGATTAATC CATCTCCAGA CACCAGGCTT 
GAGCTGAATG ATGTTGTATA TTTGATCCGA CCAGATCCAC TGTCCTACCT TCCGAACAGT 
GAACCCAGTA GGAAAAACAG CATCTGCAAT GCCGCTGTTC AAGATTCTCG GGAGGAAACA 
CAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942057.1
CDS	40..3468
Translation	MVDLESEVPPLPPRYRFRDLLLGDQGWQNDDRVQVEFYMNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLSCLLYIIRVLLEKPSQGNDWSHIFWVNRSLPLWGLQVSVALISLFETILLGYLSYKGNIWEQILRVPFILEIINAVPFIISIFWPTLRNLFVPVFLNCWLAKHALENMINDLHRAIQRTQSAMFNQVLILISTLLCLIFTCICGIQHLERIGKKLNLFDSLYFCIVTFSTVGFGDVTPETWSSKLFVVAMICVALVVLPIQFEQLAYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDVQVRRVLQIPMWSQRVIYLQGSALKDQDLLRAKMDNAEACFILSSRCEVDRTSSDHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCICPATSTLITLLVHTSRGQCVCLCCREGQQSPEQWQKTYGRCSGNEVYHIVLEESTFFAEYEGKSFTYASFHAHKKFGVCLVGVRREDNKNILLNPGPRYIMNASDICFYINITKEENSAFKNQDQQRKSNVSRSFYHGPSRLPVHSIIASMGTVAIDLQDTSCRAASGPTLALPSEGGKELRRPSIAPVLEVADTSSIQTCDLLSDQSEDETTPDEETSSNLEYAKGYPPYSPYIGSSPTFCHLLQEKVPFCCLRLDKSCQHNYYEDAKAYGFKNKLIIVAAETAGNGLYNFIVPLRAYYRPKKELNPIVLLLDNPPDMHFLDAICWFPMVYYMVGSIDNLDDLLRCGVTFAANMVVVDKESTMSAEEDYMADAKTIVNVQTLFRLFSSLSIITELTHPANMRFMQFRAKDCYSLALSKLEKKERERGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMISITRLLLGLDTIPGSGFLCSMKITEDDLWIRTYARLYQKLCSSTGDVPIGIYRTESQKLTTSESQISISVEEWEDTKDVKDPGHHRSIHRNSTSSDQSDHPLLRRKSMQWARRLSRKGPKHSGKTAEKITQQRLNLYRRSERQELAELVKNRMKHLGLSTVGYDEMNDHQSTLSYILINPSPDTRLELNDVVYLIRPDPLSYLPNSEPSRKNSICNAAVQDSREETQL

Target ORF information:

RefSeq Version	NM_198762.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198762.1

ORF Insert Sequence:

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGCA TTTGTGGAAT TCAGCATCTG 
GAACGAATTG GAAAGAAACT CAATCTCTTT GACTCCCTGT ATTTCTGCAT TGTGACATTT 
TCTACTGTGG GCTTTGGGGA TGTTACTCCT GAAACATGGT CCTCCAAGCT TTTTGTTGTT 
GCTATGATCT GCGTTGCTCT TGTAGTTCTC CCCATACAGT TTGAACAGCT GGCCTATTTG 
TGGATGGAGA GACAAAAGTC GGGAGGAAAC TATAGTCGAC ACAGAGCTCA GACTGAGAAG 
CATGTTGTTC TGTGTGTCAG CTCATTGAAG ATTGACTTGC TTATGGATTT TTTGAATGAA 
TTCTATGCAC ATCCAAGACT GCAGGATTAT TATGTGGTGA TTTTGTGTCC CACGGAGATG 
GATGTGCAAG TTCGGAGAGT GCTACAGATT CCAATGTGGT CCCAGAGAGT GATCTACCTT 
CAAGGCTCTG CCCTTAAAGA CCAGGATCTC CTGAGAGCAA AGATGGACAA TGCTGAGGCC 
TGTTTCATTT TGAGTAGCCG CTGTGAAGTC GATAGAACAT CATCTGATCA CCAGACAATT 
TTGAGAGCAT GGGCTGTAAA AGATTTTGCC CCAAATTGTC CTCTGTATGT TCAGATATTA 
AAACCAGAAA ATAAATTCCA CATCAAATTT GCAGATCATG TTGTTTGTGA AGAAGAGTTT 
AAGTACGCCA TGTTAGCTTT AAACTGTATA TGCCCAGCAA CATCTACACT TATTACACTA 
CTGGTTCATA CCTCTAGAGG GCAGTGTGTG TGCTTGTGTT GCAGAGAAGG GCAGCAGTCA 
CCTGAACAGT GGCAGAAGAC GTATGGGAGG TGCTCAGGAA ATGAAGTCTA CCACATCGTT 
CTGGAAGAAA GTACATTTTT TGCTGAATAT GAAGGGAAGA GTTTTACCTA TGCTTCTTTC 
CATGCACACA AAAAGTTTGG TGTCTGCTTG GTTGGTGTTA GGAGGGAGGA TAATAAAAAC 
ATTTTGCTGA ATCCAGGTCC TCGATACATT ATGAATGCTT CAGACATATG TTTTTATATT 
AATATTACCA AAGAAGAAAA TTCAGCATTT AAGAATCAAG ACCAACAGAG AAAAAGTAAT 
GTGTCAAGGT CATTTTATCA TGGGCCTTCC AGATTGCCTG TCCACAGTAT CATTGCCAGC 
ATGGGTACTG TGGCTATAGA CTTACAAGAC ACAAGCTGTA GAGCAGCAAG TGGCCCTACA 
CTGGCTCTTC CTTCAGAGGG AGGCAAGGAA CTAAGAAGAC CTAGCATTGC TCCTGTTTTA 
GAGGTAGCAG ATACATCATC AATTCAAACC TGCGATCTTC TAAGTGACCA GTCAGAAGAT 
GAAACTACAC CAGATGAAGA AACATCTTCA AATTTAGAGT ATGCCAAAGG CTACCCACCT 
TATTCTCCAT ACATAGGAAG TTCACCTACT TTTTGTCATC TACTTCAAGA AAAAGTGCCC 
TTTTGCTGCC TAAGATTAGA CAAGAGTTGC CAGCATAACT ACTATGAGGA TGCAAAAGCC 
TATGGATTCA AAAATAAACT AATTATAGTT GCAGCTGAAA CGGCTGGGAA CGGATTATAT 
AATTTTATTG TACCTCTCAG GGCATATTAT AGACCAAAGA AAGAATTAAA TCCCATAGTT 
CTTCTATTGG ATAATCCGCC AGATATGCAT TTTCTGGATG CAATCTGTTG GTTTCCAATG 
GTTTACTACA TGGTGGGCTC TATTGACAAC CTAGATGATT TGCTCAGGTG TGGAGTGACC 
TTCGCTGCCA ACATGGTGGT TGTGGACAAA GAAAGCACCA TGAGTGCAGA GGAAGACTAC 
ATGGCAGATG CCAAGACGAT TGTGAATGTG CAGACTCTTT TCAGGTTGTT TTCGAGTCTC 
AGTATTATCA CTGAGCTTAC TCATCCAGCA AATATGAGAT TCATGCAATT CAGAGCCAAG 
GACTGTTACT CTCTTGCACT TTCAAAACTG GAAAAGAAAG AGCGAGAACG AGGTTCTAAC 
CTGGCCTTCA TGTTCCGACT GCCTTTCGCT GCGGGGAGAG TGTTCAGCAT CAGTATGCTG 
GACACGCTGC TTTATCAGTC ATTTGTGAAA GATTATATGA TTTCTATCAC CAGACTTCTT 
TTGGGACTGG ACACCATACC AGGATCGGGG TTTCTTTGTT CTATGAAAAT TACTGAAGAT 
GACTTGTGGA TCAGAACGTA TGCCAGACTC TATCAGAAGC TGTGTTCTTC TACTGGAGAT 
GTTCCCATTG GGATCTACAG AACAGAATCT CAGAAGCTAA CTACATCTGA GTCTCAAATA 
TCCATCAGTG TGGAAGAGTG GGAAGACACC AAAGATGTCA AAGACCCAGG GCACCACCGC 
AGCATTCACC GCAACTCAAC CTCTAGCGAC CAGTCAGACC ACCCCTTGCT GCGGAGGAAG 
AGCATGCAGT GGGCCCGGAG GCTGAGCAGA AAAGGGCCAA AGCACTCTGG TAAAACTGCA 
GAAAAGATAA CCCAGCAACG ACTGAATCTC TACAGGAGGT CGGAAAGACA GGAGCTTGCT 
GAACTTGTGA AAAACAGAAT GAAACACCTG GGCCTCTCTA CGGTGGGCTA CGATGAAATG 
AATGATCATC AGAGCACCCT TTCCTACATC CTGATTAATC CATCTCCAGA CACCAGGCTT 
GAGCTGAATG ATGTTGTATA TTTGATCCGA CCAGATCCAC TGTCCTACCT TCCGAACAGT 
GAACCCAGTA GGAAAAACAG CATCTGCAAT GCCGCTGTTC AAGATTCTCG GGAGGAAACA 
CAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt2 ( XM_006249942.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250004.1 Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X4, mRNA.

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CloneID	ORa35408
Clone ID Related Accession (Same CDS sequence)	XM_006249942.3
Accession Version	XM_006249942.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium channel subfamily T member 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249942.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGCA TTTGTGGAAT TCAGCATCTG 
GAACGAATTG GAAAGAAACT CAATCTCTTT GACTCCCTTT ATTTCTGCAT TGTGACATTT 
TCTACTGTGG GCTTTGGGGA TGTTACTCCT GAAACATGGT CCTCCAAGCT TTTTGTTGTT 
GCTATGATCT GCGTTGCTCT TGTAGTTCTC CCCATACAGT TTGAACAGCT GGCCTATTTG 
TGGATGGAGA GACAAAAGTC GGGAGGAAAC TATAGTCGAC ACAGAGCTCA GACTGAGAAG 
CATGTTGTTC TGTGTGTCAG CTCATTGAAG ATTGACTTGC TTATGGATTT TTTGAATGAA 
TTCTATGCAC ATCCAAGACT GCAGGATTAT TATGTGGTGA TTTTGTGTCC CACGGAGATG 
GATGTGCAAG TTCGGAGAGT GCTACAGATT CCAATGTGGT CCCAGAGAGT GATCTACCTT 
CAAGGCTCTG CCCTTAAAGA CCAGGATCTC CTGAGAGCAA AGATGGACAA TGCTGAGGCC 
TGTTTCATTT TGAGTAGCCG CTGTGAAGTC GATAGAACAT CATCTGATCA CCAGACAATT 
TTGAGAGCAT GGGCTGTAAA AGATTTTGCC CCAAATTGTC CTCTGTATGT TCAGATATTA 
AAACCAGAAA ATAAATTCCA CATCAAATTT GCAGATCATG TTGTTTGTGA AGAAGAGTTT 
AAGTACGCCA TGTTAGCTTT AAACTGTATA TGCCCAGCAA CATCTACACT TATTACACTA 
CTGGTTCATA CCTCTAGAGG GCAAGAAGGG CAGCAGTCAC CTGAACAGTG GCAGAAGACG 
TATGGGAGGT GCTCAGGAAA TGAAGTCTAC CACATCGTTC TGGAAGAAAG TACATTTTTT 
GCTGAATATG AAGGGAAGAG TTTTACCTAT GCTTCTTTCC ATGCACACAA AAAGTTTGGT 
GTCTGCTTGG TTGGTGTTAG GAGGGAGGAT AATAAAAACA TTTTGCTGAA TCCAGGTCCT 
CGATACATTA TGAATGCTTC AGACATATGT TTTTATATTA ATATTACCAA AGAAGAAAAT 
TCAGCATTTA AGAATCAAGA CCAACAGAGA AAAAGTAATG TGTCAAGGTC ATTTTATCAT 
GGGCCTTCCA GATTGCCTGT CCACAGTATC ATTGCCAGCA TGGGTACTGT GGCTATAGAC 
TTACAAGACA CAAGCTGTAG AGCAGCAAGT GGCCCTACAC TGGCTCTTCC TTCAGAGGGA 
GGCAAGGAAC TAAGAAGACC TAGCATTGCT CCTGTTTTAG AGGTAGCAGA TACATCATCA 
ATTCAAACCT GCGATCTTCT AAGTGACCAG TCAGAAGATG AAACTACACC AGATGAAGAA 
ACATCTTCAA ATTTAGAGTA TGCCAAAGGC TACCCACCTT ATTCTCCATA CATAGGAAGT 
TCACCTACTT TTTGTCATCT ACTTCAAGAA AAAGTGCCCT TTTGCTGCTT AAGATTAGAC 
AAGAGTTGCC AGCATAACTA CTATGAGGAT GCAAAAGCCT ATGGATTCAA AAATAAACTA 
ATTATAGTTG CAGCTGAAAC GGCTGGGAAC GGATTATATA ATTTTATTGT ACCTCTCAGG 
GCATATTATA GACCAAAGAA AGAATTAAAT CCCATAGTTC TTCTATTGGA TAATCCGCCA 
GATATGCATT TTCTGGATGC AATCTGTTGG TTTCCAATGG TTTACTACAT GGTGGGCTCT 
ATTGACAACC TAGATGATTT GCTCAGGTGT GGAGTGACCT TCGCTGCCAA CATGGTGGTT 
GTGGACAAAG AAAGCACCAT GAGTGCAGAG GAAGACTACA TGGCAGATGC CAAGACGATT 
GTGAATGTGC AGACTCTTTT CAGGTTGTTT TCGAGTCTCA GTATTATCAC TGAGCTTACT 
CATCCAGCAA ATATGAGATT CATGCAATTC AGAGCCAAGG ACTGTTACTC TCTTGCACTT 
TCAAAACTGG AAAAGAAAGA GCGAGAACGA GGTTCTAACC TGGCCTTCAT GTTCCGACTG 
CCTTTCGCTG CGGGGAGAGT GTTCAGCATC AGTATGCTGG ACACGCTGCT TTATCAGTCA 
TTTGTGAAAG ATTATATGAT TTCTATCACC AGACTTCTTT TGGGACTGGA CACCATACCA 
GGATCGGGGT TTCTTTGTTC TATGAAAATT ACTGAAGATG ACTTGTGGAT CAGAACGTAT 
GCCAGACTCT ATCAGAAGCT GTGTTCTTCT ACTGGAGATG TTCCCATTGG GATCTACAGA 
ACAGAATCTC AGAAGCTAAC TACATCTGAG TCTCAAATAT CCATCAGTGT GGAAGAGTGG 
GAAGACACCA AAGATGTCAA AGACCCAGGG CACCACCGCA GCATTCACCG CAACTCAACC 
TCTAGCGACC AGTCAGACCA CCCCTTGCTG CGGAGGAAGA GCATGCAGTG GGCCCGGAGG 
CTGAGCAGAA AAGGGCCAAA GCACTCTGGT AAAACTGCAG AAAAGATAAC CCAGCAACGA 
CTGAATCTCT ACAGGAGGTC GGAAAGACAG GAGCTTGCTG AACTTGTGAA AAACAGAATG 
AAACACCTGG GCCTCTCTAC GGTGGGCTAC GATGAAATGA ATGATCATCA GAGCACCCTT 
TCCTACATCC TGATTAATCC ATCTCCAGAC ACCAGGCTTG AGCTGAATGA TGTTGTATAT 
TTGATCCGAC CAGATCCACT GTCCTACCTT CCGAACAGTG AACCCAGTAG GAAAAACAGC 
ATCTGCAATG CCGCTGTTCA AGATTCTCGG GAGGAAACAC AACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250004.1
CDS	578..3985
Translation	MVDLESEVPPLPPRYRFRDLLLGDQGWQNDDRVQVEFYMNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLSCLLYIIRVLLEKPSQGNDWSHIFWVNRSLPLWGLQVSVALISLFETILLGYLSYKGNIWEQILRVPFILEIINAVPFIISIFWPTLRNLFVPVFLNCWLAKHALENMINDLHRAIQRTQSAMFNQVLILISTLLCLIFTCICGIQHLERIGKKLNLFDSLYFCIVTFSTVGFGDVTPETWSSKLFVVAMICVALVVLPIQFEQLAYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDVQVRRVLQIPMWSQRVIYLQGSALKDQDLLRAKMDNAEACFILSSRCEVDRTSSDHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCICPATSTLITLLVHTSRGQEGQQSPEQWQKTYGRCSGNEVYHIVLEESTFFAEYEGKSFTYASFHAHKKFGVCLVGVRREDNKNILLNPGPRYIMNASDICFYINITKEENSAFKNQDQQRKSNVSRSFYHGPSRLPVHSIIASMGTVAIDLQDTSCRAASGPTLALPSEGGKELRRPSIAPVLEVADTSSIQTCDLLSDQSEDETTPDEETSSNLEYAKGYPPYSPYIGSSPTFCHLLQEKVPFCCLRLDKSCQHNYYEDAKAYGFKNKLIIVAAETAGNGLYNFIVPLRAYYRPKKELNPIVLLLDNPPDMHFLDAICWFPMVYYMVGSIDNLDDLLRCGVTFAANMVVVDKESTMSAEEDYMADAKTIVNVQTLFRLFSSLSIITELTHPANMRFMQFRAKDCYSLALSKLEKKERERGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMISITRLLLGLDTIPGSGFLCSMKITEDDLWIRTYARLYQKLCSSTGDVPIGIYRTESQKLTTSESQISISVEEWEDTKDVKDPGHHRSIHRNSTSSDQSDHPLLRRKSMQWARRLSRKGPKHSGKTAEKITQQRLNLYRRSERQELAELVKNRMKHLGLSTVGYDEMNDHQSTLSYILINPSPDTRLELNDVVYLIRPDPLSYLPNSEPSRKNSICNAAVQDSREETQL

Target ORF information:

RefSeq Version	XM_006249942.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249942.3

ORF Insert Sequence:

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGCA TTTGTGGAAT TCAGCATCTG 
GAACGAATTG GAAAGAAACT CAATCTCTTT GACTCCCTTT ATTTCTGCAT TGTGACATTT 
TCTACTGTGG GCTTTGGGGA TGTTACTCCT GAAACATGGT CCTCCAAGCT TTTTGTTGTT 
GCTATGATCT GCGTTGCTCT TGTAGTTCTC CCCATACAGT TTGAACAGCT GGCCTATTTG 
TGGATGGAGA GACAAAAGTC GGGAGGAAAC TATAGTCGAC ACAGAGCTCA GACTGAGAAG 
CATGTTGTTC TGTGTGTCAG CTCATTGAAG ATTGACTTGC TTATGGATTT TTTGAATGAA 
TTCTATGCAC ATCCAAGACT GCAGGATTAT TATGTGGTGA TTTTGTGTCC CACGGAGATG 
GATGTGCAAG TTCGGAGAGT GCTACAGATT CCAATGTGGT CCCAGAGAGT GATCTACCTT 
CAAGGCTCTG CCCTTAAAGA CCAGGATCTC CTGAGAGCAA AGATGGACAA TGCTGAGGCC 
TGTTTCATTT TGAGTAGCCG CTGTGAAGTC GATAGAACAT CATCTGATCA CCAGACAATT 
TTGAGAGCAT GGGCTGTAAA AGATTTTGCC CCAAATTGTC CTCTGTATGT TCAGATATTA 
AAACCAGAAA ATAAATTCCA CATCAAATTT GCAGATCATG TTGTTTGTGA AGAAGAGTTT 
AAGTACGCCA TGTTAGCTTT AAACTGTATA TGCCCAGCAA CATCTACACT TATTACACTA 
CTGGTTCATA CCTCTAGAGG GCAAGAAGGG CAGCAGTCAC CTGAACAGTG GCAGAAGACG 
TATGGGAGGT GCTCAGGAAA TGAAGTCTAC CACATCGTTC TGGAAGAAAG TACATTTTTT 
GCTGAATATG AAGGGAAGAG TTTTACCTAT GCTTCTTTCC ATGCACACAA AAAGTTTGGT 
GTCTGCTTGG TTGGTGTTAG GAGGGAGGAT AATAAAAACA TTTTGCTGAA TCCAGGTCCT 
CGATACATTA TGAATGCTTC AGACATATGT TTTTATATTA ATATTACCAA AGAAGAAAAT 
TCAGCATTTA AGAATCAAGA CCAACAGAGA AAAAGTAATG TGTCAAGGTC ATTTTATCAT 
GGGCCTTCCA GATTGCCTGT CCACAGTATC ATTGCCAGCA TGGGTACTGT GGCTATAGAC 
TTACAAGACA CAAGCTGTAG AGCAGCAAGT GGCCCTACAC TGGCTCTTCC TTCAGAGGGA 
GGCAAGGAAC TAAGAAGACC TAGCATTGCT CCTGTTTTAG AGGTAGCAGA TACATCATCA 
ATTCAAACCT GCGATCTTCT AAGTGACCAG TCAGAAGATG AAACTACACC AGATGAAGAA 
ACATCTTCAA ATTTAGAGTA TGCCAAAGGC TACCCACCTT ATTCTCCATA CATAGGAAGT 
TCACCTACTT TTTGTCATCT ACTTCAAGAA AAAGTGCCCT TTTGCTGCTT AAGATTAGAC 
AAGAGTTGCC AGCATAACTA CTATGAGGAT GCAAAAGCCT ATGGATTCAA AAATAAACTA 
ATTATAGTTG CAGCTGAAAC GGCTGGGAAC GGATTATATA ATTTTATTGT ACCTCTCAGG 
GCATATTATA GACCAAAGAA AGAATTAAAT CCCATAGTTC TTCTATTGGA TAATCCGCCA 
GATATGCATT TTCTGGATGC AATCTGTTGG TTTCCAATGG TTTACTACAT GGTGGGCTCT 
ATTGACAACC TAGATGATTT GCTCAGGTGT GGAGTGACCT TCGCTGCCAA CATGGTGGTT 
GTGGACAAAG AAAGCACCAT GAGTGCAGAG GAAGACTACA TGGCAGATGC CAAGACGATT 
GTGAATGTGC AGACTCTTTT CAGGTTGTTT TCGAGTCTCA GTATTATCAC TGAGCTTACT 
CATCCAGCAA ATATGAGATT CATGCAATTC AGAGCCAAGG ACTGTTACTC TCTTGCACTT 
TCAAAACTGG AAAAGAAAGA GCGAGAACGA GGTTCTAACC TGGCCTTCAT GTTCCGACTG 
CCTTTCGCTG CGGGGAGAGT GTTCAGCATC AGTATGCTGG ACACGCTGCT TTATCAGTCA 
TTTGTGAAAG ATTATATGAT TTCTATCACC AGACTTCTTT TGGGACTGGA CACCATACCA 
GGATCGGGGT TTCTTTGTTC TATGAAAATT ACTGAAGATG ACTTGTGGAT CAGAACGTAT 
GCCAGACTCT ATCAGAAGCT GTGTTCTTCT ACTGGAGATG TTCCCATTGG GATCTACAGA 
ACAGAATCTC AGAAGCTAAC TACATCTGAG TCTCAAATAT CCATCAGTGT GGAAGAGTGG 
GAAGACACCA AAGATGTCAA AGACCCAGGG CACCACCGCA GCATTCACCG CAACTCAACC 
TCTAGCGACC AGTCAGACCA CCCCTTGCTG CGGAGGAAGA GCATGCAGTG GGCCCGGAGG 
CTGAGCAGAA AAGGGCCAAA GCACTCTGGT AAAACTGCAG AAAAGATAAC CCAGCAACGA 
CTGAATCTCT ACAGGAGGTC GGAAAGACAG GAGCTTGCTG AACTTGTGAA AAACAGAATG 
AAACACCTGG GCCTCTCTAC GGTGGGCTAC GATGAAATGA ATGATCATCA GAGCACCCTT 
TCCTACATCC TGATTAATCC ATCTCCAGAC ACCAGGCTTG AGCTGAATGA TGTTGTATAT 
TTGATCCGAC CAGATCCACT GTCCTACCTT CCGAACAGTG AACCCAGTAG GAAAAACAGC 
ATCTGCAATG CCGCTGTTCA AGATTCTCGG GAGGAAACAC AACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt2 ( XM_006249943.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250005.1 Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X5, mRNA.

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CloneID	ORa35409
Clone ID Related Accession (Same CDS sequence)	XM_006249943.3
Accession Version	XM_006249943.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium channel subfamily T member 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249943.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGCA TTTGTGGAAT TCAGCATCTG 
GAACGAATTG GAAAGAAACT CAATCTCTTT GACTCCCTTT ATTTCTGCAT TGTGACATTT 
TCTACTGTGG GCTTTGGGGA TGTTACTCCT GAAACATGGT CCTCCAAGCT TTTTGTTGTT 
GCTATGATCT GCGTTGCTCT TGTAGTTCTC CCCATACAGT TTGAACAGCT GGCCTATTTG 
TGGATGGAGA GACAAAAGTC GGGAGGAAAC TATAGTCGAC ACAGAGCTCA GACTGAGAAG 
CATGTTGTTC TGTGTGTCAG CTCATTGAAG ATTGACTTGC TTATGGATTT TTTGAATGAA 
TTCTATGCAC ATCCAAGACT GCAGGATTAT TATGTGGTGA TTTTGTGTCC CACGGAGATG 
GATGTGCAAG TTCGGAGAGT GCTACAGATT CCAATGTGGT CCCAGAGAGT GATCTACCTT 
CAAGGCTCTG CCCTTAAAGA CCAGGATCTC CTGAGAGCAA AGATGGACAA TGCTGAGGCC 
TGTTTCATTT TGAGTAGCCG CTGTGAAGTC GATAGAACAT CATCTGATCA CCAGACAATT 
TTGAGAGCAT GGGCTGTAAA AGATTTTGCC CCAAATTGTC CTCTGTATGT TCAGATATTA 
AAACCAGAAA ATAAATTCCA CATCAAATTT GCAGATCATG TTGTTTGTGA AGAAGAGTTT 
AAGTACGCCA TGTTAGCTTT AAACTGTATA TGCCCAGCAA CATCTACACT TATTACACTA 
CTGGTTCATA CCTCTAGAGG GCAAGAAGGG CAGCAGTCAC CTGAACAGTG GCAGAAGACG 
TATGGGAGGT GCTCAGGAAA TGAAGTCTAC CACATCGTTC TGGAAGAAAG TACATTTTTT 
GCTGAATATG AAGGGAAGAG TTTTACCTAT GCTTCTTTCC ATGCACACAA AAAGTTTGGT 
GTCTGCTTGG TTGGTGTTAG GAGGGAGGAT AATAAAAACA TTTTGCTGAA TCCAGGTCCT 
CGATACATTA TGAATGCTTC AGACATATGT TTTTATATTA ATATTACCAA AGAAGAAAAT 
TCAGCATTTA AGAATCAAGA CCAACAGAGA AAAAGTAATG TGTCAAGGTC ATTTTATCAT 
GGGCCTTCCA GATTGCCTGT CCACAGTATC ATTGCCAGCA TGGGTACTGT GGCTATAGAC 
TTACAAGACA CAAGCTGTAG AGCAGCAAGT GGCCCTACAC TGGCTCTTCC TTCAGAGGGA 
GGCAAGGAAC TAAGAAGACC TAGCATTGCT CCTGTTTTAG AGGTAGCAGA TACATCATCA 
ATTCAAACCT GCGATCTTCT AAGTGACCAG TCAGAAGATG AAACTACACC AGATGAAGAA 
ACATCTTCAA ATTTAGAGTA TGCCAAAGGC TACCCACCTT ATTCTCCATA CATAGGAAGT 
TCACCTACTT TTTGTCATCT ACTTCAAGAA AAAGTGCCCT TTTGCTGCTT AAGATTAGAC 
AAGAGTTGCC AGCATAACTA CTATGAGGAT GCAAAAGCCT ATGGATTCAA AAATAAACTA 
ATTATAGTTG CAGCTGAAAC GGCTGGGAAC GGATTATATA ATTTTATTGT ACCTCTCAGG 
GCATATTATA GACCAAAGAA AGAATTAAAT CCCATAGTTC TTCTATTGGA TAATCCCCTA 
GATGATTTGC TCAGGTGTGG AGTGACCTTC GCTGCCAACA TGGTGGTTGT GGACAAAGAA 
AGCACCATGA GTGCAGAGGA AGACTACATG GCAGATGCCA AGACGATTGT GAATGTGCAG 
ACTCTTTTCA GGTTGTTTTC GAGTCTCAGT ATTATCACTG AGCTTACTCA TCCAGCAAAT 
ATGAGATTCA TGCAATTCAG AGCCAAGGAC TGTTACTCTC TTGCACTTTC AAAACTGGAA 
AAGAAAGAGC GAGAACGAGG TTCTAACCTG GCCTTCATGT TCCGACTGCC TTTCGCTGCG 
GGGAGAGTGT TCAGCATCAG TATGCTGGAC ACGCTGCTTT ATCAGTCATT TGTGAAAGAT 
TATATGATTT CTATCACCAG ACTTCTTTTG GGACTGGACA CCATACCAGG ATCGGGGTTT 
CTTTGTTCTA TGAAAATTAC TGAAGATGAC TTGTGGATCA GAACGTATGC CAGACTCTAT 
CAGAAGCTGT GTTCTTCTAC TGGAGATGTT CCCATTGGGA TCTACAGAAC AGAATCTCAG 
AAGCTAACTA CATCTGAGTC TCAAATATCC ATCAGTGTGG AAGAGTGGGA AGACACCAAA 
GATGTCAAAG ACCCAGGGCA CCACCGCAGC ATTCACCGCA ACTCAACCTC TAGCGACCAG 
TCAGACCACC CCTTGCTGCG GAGGAAGAGC ATGCAGTGGG CCCGGAGGCT GAGCAGAAAA 
GGGCCAAAGC ACTCTGGTAA AACTGCAGAA AAGATAACCC AGCAACGACT GAATCTCTAC 
AGGAGGTCGG AAAGACAGGA GCTTGCTGAA CTTGTGAAAA ACAGAATGAA ACACCTGGGC 
CTCTCTACGG TGGGCTACGA TGAAATGAAT GATCATCAGA GCACCCTTTC CTACATCCTG 
ATTAATCCAT CTCCAGACAC CAGGCTTGAG CTGAATGATG TTGTATATTT GATCCGACCA 
GATCCACTGT CCTACCTTCC GAACAGTGAA CCCAGTAGGA AAAACAGCAT CTGCAATGCC 
GCTGTTCAAG ATTCTCGGGA GGAAACACAA CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250005.1
CDS	578..3913
Translation	MVDLESEVPPLPPRYRFRDLLLGDQGWQNDDRVQVEFYMNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLSCLLYIIRVLLEKPSQGNDWSHIFWVNRSLPLWGLQVSVALISLFETILLGYLSYKGNIWEQILRVPFILEIINAVPFIISIFWPTLRNLFVPVFLNCWLAKHALENMINDLHRAIQRTQSAMFNQVLILISTLLCLIFTCICGIQHLERIGKKLNLFDSLYFCIVTFSTVGFGDVTPETWSSKLFVVAMICVALVVLPIQFEQLAYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDVQVRRVLQIPMWSQRVIYLQGSALKDQDLLRAKMDNAEACFILSSRCEVDRTSSDHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCICPATSTLITLLVHTSRGQEGQQSPEQWQKTYGRCSGNEVYHIVLEESTFFAEYEGKSFTYASFHAHKKFGVCLVGVRREDNKNILLNPGPRYIMNASDICFYINITKEENSAFKNQDQQRKSNVSRSFYHGPSRLPVHSIIASMGTVAIDLQDTSCRAASGPTLALPSEGGKELRRPSIAPVLEVADTSSIQTCDLLSDQSEDETTPDEETSSNLEYAKGYPPYSPYIGSSPTFCHLLQEKVPFCCLRLDKSCQHNYYEDAKAYGFKNKLIIVAAETAGNGLYNFIVPLRAYYRPKKELNPIVLLLDNPLDDLLRCGVTFAANMVVVDKESTMSAEEDYMADAKTIVNVQTLFRLFSSLSIITELTHPANMRFMQFRAKDCYSLALSKLEKKERERGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMISITRLLLGLDTIPGSGFLCSMKITEDDLWIRTYARLYQKLCSSTGDVPIGIYRTESQKLTTSESQISISVEEWEDTKDVKDPGHHRSIHRNSTSSDQSDHPLLRRKSMQWARRLSRKGPKHSGKTAEKITQQRLNLYRRSERQELAELVKNRMKHLGLSTVGYDEMNDHQSTLSYILINPSPDTRLELNDVVYLIRPDPLSYLPNSEPSRKNSICNAAVQDSREETQL

Target ORF information:

RefSeq Version	XM_006249943.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium sodium-activated channel subfamily T member 2 (Kcnt2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249943.3

ORF Insert Sequence:

ATGGTTGATT TGGAGAGCGA AGTACCCCCT CTGCCTCCCA GGTACAGATT CCGTGATTTG 
CTGCTAGGGG ACCAAGGCTG GCAGAATGAT GACAGGGTTC AAGTCGAATT CTACATGAAT 
GAAAACACAT TTAAAGAGAG ATTAAAGTTG TTTTTCATAA AAAACCAGCG ATCAAGTCTA 
AGAATCCGCT TGTTCAACTT TTCCCTCAAA TTGTTAAGCT GTTTGTTATA CATTATCCGT 
GTGTTGCTTG AGAAACCTTC ACAGGGAAAT GATTGGTCTC ACATATTTTG GGTTAACAGA 
AGCCTGCCTT TATGGGGTTT ACAGGTCTCG GTGGCATTGA TAAGTCTATT TGAAACAATA 
CTCCTTGGAT ATCTCAGTTA TAAGGGGAAT ATCTGGGAGC AGATTTTACG AGTTCCATTC 
ATCTTGGAAA TAATCAATGC AGTTCCCTTC ATCATCTCAA TATTCTGGCC TACGTTAAGA 
AATCTCTTTG TTCCAGTTTT TCTAAATTGT TGGCTTGCCA AACATGCCTT GGAAAATATG 
ATTAATGATC TTCATCGAGC CATTCAGCGT ACACAGTCTG CAATGTTTAA TCAAGTTCTG 
ATTTTGATAT CTACTTTACT ATGCCTTATC TTCACTTGCA TTTGTGGAAT TCAGCATCTG 
GAACGAATTG GAAAGAAACT CAATCTCTTT GACTCCCTTT ATTTCTGCAT TGTGACATTT 
TCTACTGTGG GCTTTGGGGA TGTTACTCCT GAAACATGGT CCTCCAAGCT TTTTGTTGTT 
GCTATGATCT GCGTTGCTCT TGTAGTTCTC CCCATACAGT TTGAACAGCT GGCCTATTTG 
TGGATGGAGA GACAAAAGTC GGGAGGAAAC TATAGTCGAC ACAGAGCTCA GACTGAGAAG 
CATGTTGTTC TGTGTGTCAG CTCATTGAAG ATTGACTTGC TTATGGATTT TTTGAATGAA 
TTCTATGCAC ATCCAAGACT GCAGGATTAT TATGTGGTGA TTTTGTGTCC CACGGAGATG 
GATGTGCAAG TTCGGAGAGT GCTACAGATT CCAATGTGGT CCCAGAGAGT GATCTACCTT 
CAAGGCTCTG CCCTTAAAGA CCAGGATCTC CTGAGAGCAA AGATGGACAA TGCTGAGGCC 
TGTTTCATTT TGAGTAGCCG CTGTGAAGTC GATAGAACAT CATCTGATCA CCAGACAATT 
TTGAGAGCAT GGGCTGTAAA AGATTTTGCC CCAAATTGTC CTCTGTATGT TCAGATATTA 
AAACCAGAAA ATAAATTCCA CATCAAATTT GCAGATCATG TTGTTTGTGA AGAAGAGTTT 
AAGTACGCCA TGTTAGCTTT AAACTGTATA TGCCCAGCAA CATCTACACT TATTACACTA 
CTGGTTCATA CCTCTAGAGG GCAAGAAGGG CAGCAGTCAC CTGAACAGTG GCAGAAGACG 
TATGGGAGGT GCTCAGGAAA TGAAGTCTAC CACATCGTTC TGGAAGAAAG TACATTTTTT 
GCTGAATATG AAGGGAAGAG TTTTACCTAT GCTTCTTTCC ATGCACACAA AAAGTTTGGT 
GTCTGCTTGG TTGGTGTTAG GAGGGAGGAT AATAAAAACA TTTTGCTGAA TCCAGGTCCT 
CGATACATTA TGAATGCTTC AGACATATGT TTTTATATTA ATATTACCAA AGAAGAAAAT 
TCAGCATTTA AGAATCAAGA CCAACAGAGA AAAAGTAATG TGTCAAGGTC ATTTTATCAT 
GGGCCTTCCA GATTGCCTGT CCACAGTATC ATTGCCAGCA TGGGTACTGT GGCTATAGAC 
TTACAAGACA CAAGCTGTAG AGCAGCAAGT GGCCCTACAC TGGCTCTTCC TTCAGAGGGA 
GGCAAGGAAC TAAGAAGACC TAGCATTGCT CCTGTTTTAG AGGTAGCAGA TACATCATCA 
ATTCAAACCT GCGATCTTCT AAGTGACCAG TCAGAAGATG AAACTACACC AGATGAAGAA 
ACATCTTCAA ATTTAGAGTA TGCCAAAGGC TACCCACCTT ATTCTCCATA CATAGGAAGT 
TCACCTACTT TTTGTCATCT ACTTCAAGAA AAAGTGCCCT TTTGCTGCTT AAGATTAGAC 
AAGAGTTGCC AGCATAACTA CTATGAGGAT GCAAAAGCCT ATGGATTCAA AAATAAACTA 
ATTATAGTTG CAGCTGAAAC GGCTGGGAAC GGATTATATA ATTTTATTGT ACCTCTCAGG 
GCATATTATA GACCAAAGAA AGAATTAAAT CCCATAGTTC TTCTATTGGA TAATCCCCTA 
GATGATTTGC TCAGGTGTGG AGTGACCTTC GCTGCCAACA TGGTGGTTGT GGACAAAGAA 
AGCACCATGA GTGCAGAGGA AGACTACATG GCAGATGCCA AGACGATTGT GAATGTGCAG 
ACTCTTTTCA GGTTGTTTTC GAGTCTCAGT ATTATCACTG AGCTTACTCA TCCAGCAAAT 
ATGAGATTCA TGCAATTCAG AGCCAAGGAC TGTTACTCTC TTGCACTTTC AAAACTGGAA 
AAGAAAGAGC GAGAACGAGG TTCTAACCTG GCCTTCATGT TCCGACTGCC TTTCGCTGCG 
GGGAGAGTGT TCAGCATCAG TATGCTGGAC ACGCTGCTTT ATCAGTCATT TGTGAAAGAT 
TATATGATTT CTATCACCAG ACTTCTTTTG GGACTGGACA CCATACCAGG ATCGGGGTTT 
CTTTGTTCTA TGAAAATTAC TGAAGATGAC TTGTGGATCA GAACGTATGC CAGACTCTAT 
CAGAAGCTGT GTTCTTCTAC TGGAGATGTT CCCATTGGGA TCTACAGAAC AGAATCTCAG 
AAGCTAACTA CATCTGAGTC TCAAATATCC ATCAGTGTGG AAGAGTGGGA AGACACCAAA 
GATGTCAAAG ACCCAGGGCA CCACCGCAGC ATTCACCGCA ACTCAACCTC TAGCGACCAG 
TCAGACCACC CCTTGCTGCG GAGGAAGAGC ATGCAGTGGG CCCGGAGGCT GAGCAGAAAA 
GGGCCAAAGC ACTCTGGTAA AACTGCAGAA AAGATAACCC AGCAACGACT GAATCTCTAC 
AGGAGGTCGG AAAGACAGGA GCTTGCTGAA CTTGTGAAAA ACAGAATGAA ACACCTGGGC 
CTCTCTACGG TGGGCTACGA TGAAATGAAT GATCATCAGA GCACCCTTTC CTACATCCTG 
ATTAATCCAT CTCCAGACAC CAGGCTTGAG CTGAATGATG TTGTATATTT GATCCGACCA 
GATCCACTGT CCTACCTTCC GAACAGTGAA CCCAGTAGGA AAAACAGCAT CTGCAATGCC 
GCTGTTCAAG ATTCTCGGGA GGAAACACAA CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A De Novo Mutation in the Sodium-Activated Potassium Channel KCNT2 Alters Ion Selectivity and Causes Epileptic Encephalopathy.
Cell reports21(4)926-933(2017 Oct)
Gururaj S,Palmer EE,Sheehan GD,Kandula T,Macintosh R,Ying K,Morris P,Tao J,Dias KR,Zhu Y,Dinger ME,Cowley MJ,Kirk EP,Roscioli T,Sachdev R,Duffey ME,Bye A,Bhattacharjee A

SLO2 Channels Are Inhibited by All Divalent Cations That Activate SLO1 K+ Channels.
The Journal of biological chemistry291(14)7347-56(2016 Apr)
Budelli G,Sun Q,Ferreira J,Butler A,Santi CM,Salkoff L

Transcriptional Regulation of the Sodium-activated Potassium Channel SLICK (KCNT2) Promoter by Nuclear Factor-?B.
The Journal of biological chemistry290(30)18575-83(2015 Jul)
Tomasello DL,Gancarz-Kausch AM,Dietz DM,Bhattacharjee A

Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels.
PloS one9(10)e110833(2014)
Tejada MA,Stople K,Hammami Bomholtz S,Meinild AK,Poulsen AN,Klaerke DA

The N-terminal domain of Slack determines the formation and trafficking of Slick/Slack heteromeric sodium-activated potassium channels.
The Journal of neuroscience : the official journal of the Society for Neuroscience29(17)5654-65(2009 Apr)
Chen H,Kronengold J,Yan Y,Gazula VR,Brown MR,Ma L,Ferreira G,Yang Y,Bhattacharjee A,Sigworth FJ,Salkoff L,Kaczmarek LK

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Kcnt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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