Mrpl12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mrpl12
Entrez Gene ID	303746
Full Name	mitochondrial ribosomal protein L12
Synonyms	Rpm12
General protein information	Preferred Names mitochondrial ribosomal protein L12 Names 39S ribosomal protein L12, mitochondrial ribosomal protein, mitochondrial, L12
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.3
Summary	a mitochondrial ribosomal protein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001029900.1	NP_001025071.1	39S ribosomal protein L12, mitochondrial

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MRPL12	NP_963900.1
Mus musculus (house mouse)	Mrpl12	NP_081480.2
Neurospora crassa	NCU06469	XP_957360.1
Pan troglodytes (chimpanzee)	MRPL12	XP_001163363.1
Canis lupus familiaris (dog)	MRPL12	XP_850016.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011581	XP_309929.3
Gallus gallus (chicken)	MRPL12	XP_001232214.2
Drosophila melanogaster (fruit fly)	mRpL12	NP_524742.1
Caenorhabditis elegans (roundworm)	mrpl-12	NP_499360.1
Saccharomyces cerevisiae (baker's yeast)	MNP1	NP_011447.1
Kluyveromyces lactis	KLLA0D03212g	XP_453209.1
Magnaporthe oryzae (rice blast fungus)	MGG_06378	XP_003717196.1
Xenopus tropicalis (tropical clawed frog)	mrpl12	NP_001006886.1
Danio rerio (zebrafish)	mrpl12	NP_001017696.1
Schizosaccharomyces pombe (fission yeast)	SPCC4B3.09c	NP_588081.2
Homo sapiens (human)	MRPL12	NP_002940.2
	MRPL12	XP_001111712.1
Rattus norvegicus (Norway rat)	Mrpl12	NP_001025071.1
Eremothecium gossypii	AGOS_AFL089C	NP_985459.1

Gene Ontology
Bibliography

Related articles in PubMed

A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome.
Richter R, Rorbach J, Pajak A, Smith PM, Wessels HJ, Huynen MA, Smeitink JA, Lightowlers RN, Chrzanowska-Lightowlers ZM
The EMBO journal29(6)1116-25(2010 Mar)

Human mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expression.
Wang Z, Cotney J, Shadel GS
The Journal of biological chemistry282(17)12610-8(2007 Apr)

Identification of mammalian mitochondrial ribosomal proteins (MRPs) by N-terminal sequencing of purified bovine MRPs and comparison to data bank sequences: the large subribosomal particle.
Graack HR, Bryant ML, O'Brien TW
Biochemistry38(50)16569-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mrpl12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mrpl12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07981	NM_001029900.1 Latest version!	Rattus norvegicus mitochondrial ribosomal protein L12 (Mrpl12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mrpl12 ( NM_001029900.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001025071.1 Rattus norvegicus mitochondrial ribosomal protein L12 (Mrpl12), mRNA.

Price & Availability↑

CloneID	ORa07981
Clone ID Related Accession (Same CDS sequence)	NM_001029900.1
Accession Version	NM_001029900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	39S ribosomal protein L12, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC131537.5. On Jul 28, 2005 this sequence version replaced XM_221202.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: FQ228179.1, FQ233456.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCCGCGGA GGGCTGCAGG AGAAAGCTCC GACCCAGTAC TATTTCCGGG CCCAGACTGC 
GTCTGCGCGT CGCGCCGCCT AGTGCGTAAT ATCAGAAGCC AACCAAGTCG CCCTGGCCGC 
CAAGGCCTGG TGGCTAGAGC GGCCCGTCCA GCTCGCCTCC TCCCGGATAG AGCGTCGCTT 
CCGCTCAGAG CCGCGTGTGA CCTTCCCGCT TGCGGAGCTA TGCTGCCGGC GGCGGCGGCG 
GCGGCTAGCC GCCTGTGGGG GCCGCGGCTT GGACTCGGGG GATCAGCGCT CCGCCTTGTC 
AGGCAACAGA TGCCCGGTGT CTGTGTGGCG CGGCAATTGA GAAGCAGCAG CCACCGAAGG 
AGTGAAGCAC TCGCCGGTGC ACCCCTGGAT AATGCTCCCA AGGAGTATCC CCCCAAGATC 
CAGCAGCTGG TCCAAGACAT TGCCAGTCTT ACGCTCCTGG AGATCTCAGA CCTCAATGAA 
CTCCTGAAGA AAACATTGAA GATCCAGGAT GTCGGCCTTA TGCCAATGGG TGGCATGGTG 
CCTGGTGCCG TCCCTGCTGC AGCACCAGCC CCTGAGGCGG CCGAGGAAGA AGACCTCCCT 
AAGCAGAAAG AGCAGACACA CTTCACGGTG CGCCTGACGG AAGCGAAGCC TGTGGACAAA 
GTGAAGCTGA TCAAGGAGAT CAAGAACTAC GTTCAGGGCA TAAACCTTGT GCAGGCCAAG 
AAGCTGGTGG AGTCCCTGCC CCAGGAAATC AAAGCTAACG TCGCCAAAGC TGAGGCAGAG 
AAGATCAAGG CAGCCTTGGA GGCAGTGGGT GGCACTGTGG TTCTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025071.1
CDS	1..831
Translation	MPRRAAGESSDPVLFPGPDCVCASRRLVRNIRSQPSRPGRQGLVARAARPARLLPDRASLPLRAACDLPACGAMLPAAAAAASRLWGPRLGLGGSALRLVRQQMPGVCVARQLRSSSHRRSEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLMPMGGMVPGAVPAAAPAPEAAEEEDLPKQKEQTHFTVRLTEAKPVDKVKLIKEIKNYVQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE

Target ORF information:

RefSeq Version	NM_001029900.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitochondrial ribosomal protein L12 (Mrpl12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029900.1

ORF Insert Sequence:

ATGCCGCGGA GGGCTGCAGG AGAAAGCTCC GACCCAGTAC TATTTCCGGG CCCAGACTGC 
GTCTGCGCGT CGCGCCGCCT AGTGCGTAAT ATCAGAAGCC AACCAAGTCG CCCTGGCCGC 
CAAGGCCTGG TGGCTAGAGC GGCCCGTCCA GCTCGCCTCC TCCCGGATAG AGCGTCGCTT 
CCGCTCAGAG CCGCGTGTGA CCTTCCCGCT TGCGGAGCTA TGCTGCCGGC GGCGGCGGCG 
GCGGCTAGCC GCCTGTGGGG GCCGCGGCTT GGACTCGGGG GATCAGCGCT CCGCCTTGTC 
AGGCAACAGA TGCCCGGTGT CTGTGTGGCG CGGCAATTGA GAAGCAGCAG CCACCGAAGG 
AGTGAAGCAC TCGCCGGTGC ACCCCTGGAT AATGCTCCCA AGGAGTATCC CCCCAAGATC 
CAGCAGCTGG TCCAAGACAT TGCCAGTCTT ACGCTCCTGG AGATCTCAGA CCTCAATGAA 
CTCCTGAAGA AAACATTGAA GATCCAGGAT GTCGGCCTTA TGCCAATGGG TGGCATGGTG 
CCTGGTGCCG TCCCTGCTGC AGCACCAGCC CCTGAGGCGG CCGAGGAAGA AGACCTCCCT 
AAGCAGAAAG AGCAGACACA CTTCACGGTG CGCCTGACGG AAGCGAAGCC TGTGGACAAA 
GTGAAGCTGA TCAAGGAGAT CAAGAACTAC GTTCAGGGCA TAAACCTTGT GCAGGCCAAG 
AAGCTGGTGG AGTCCCTGCC CCAGGAAATC AAAGCTAACG TCGCCAAAGC TGAGGCAGAG 
AAGATCAAGG CAGCCTTGGA GGCAGTGGGT GGCACTGTGG TTCTGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome.
The EMBO journal29(6)1116-25(2010 Mar)
Richter R,Rorbach J,Pajak A,Smith PM,Wessels HJ,Huynen MA,Smeitink JA,Lightowlers RN,Chrzanowska-Lightowlers ZM

Human mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expression.
The Journal of biological chemistry282(17)12610-8(2007 Apr)
Wang Z,Cotney J,Shadel GS

Identification of mammalian mitochondrial ribosomal proteins (MRPs) by N-terminal sequencing of purified bovine MRPs and comparison to data bank sequences: the large subribosomal particle.
Biochemistry38(50)16569-77(1999 Dec)
Graack HR,Bryant ML,O'Brien TW