Fastkd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fastkd2
Entrez Gene ID	301463
Full Name	FAST kinase domains 2
Synonyms	RGD1307883
General protein information	Preferred Names FAST kinase domains 2 Names FAST kinase domain-containing protein 2, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_006245050.2	XP_006245112.2	FAST kinase domain-containing protein 2, mitochondrial isoform X1
NM_001009673.1	NP_001009673.1	FAST kinase domain-containing protein 2, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FASTKD2	XP_003309502.1
	FASTKD2	XP_002799081.1
Bos taurus (cattle)	FASTKD2	NP_001095587.1
Danio rerio (zebrafish)	fastkd2	XP_692810.5
Xenopus tropicalis (tropical clawed frog)	fastkd2	XP_002937162.1
Mus musculus (house mouse)	Fastkd2	NP_766010.1
Gallus gallus (chicken)	FASTKD2	XP_421951.2
Homo sapiens (human)	FASTKD2	NP_001129665.1
Canis lupus familiaris (dog)	FASTKD2	XP_005640642.1
Rattus norvegicus (Norway rat)	Fastkd2	NP_001009673.1

Gene Ontology
Bibliography

Related articles in PubMed

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.
Arroyo JD, Jourdain AA, Calvo SE, Ballarano CA, Doench JG, Root DE, Mootha VK
Cell metabolism24(6)875-885(2016 Dec)

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.
Antonicka H, Shoubridge EA
Cell reports(2015 Feb)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Fast kinase domain-containing protein 3 is a mitochondrial protein essential for cellular respiration.
Simarro M, Gimenez-Cassina A, Kedersha N, Lazaro JB, Adelmant GO, Marto JA, Rhee K, Tisdale S, Danial N, Benarafa C, Ordu?a A, Anderson P
Biochemical and biophysical research communications401(3)440-6(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fastkd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fastkd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00788	NM_001009673.1 Latest version!	Rattus norvegicus FAST kinase domains 2 (Fastkd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa62726	XM_006245050.2 Latest version!	Rattus norvegicus FAST kinase domains 2 (Fastkd2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fastkd2 ( NM_001009673.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009673.1 Rattus norvegicus FAST kinase domains 2 (Fastkd2), mRNA.

Price & Availability↑

CloneID	ORa00788
Clone ID Related Accession (Same CDS sequence)	NM_001009673.1
Accession Version	NM_001009673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	FAST kinase domain-containing protein 2, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088416.1. On Jan 13, 2005 this sequence version replaced XM_217415.2. ##Evidence-Data-START## Transcript exon combination :: BC088416.1 [ECO:0000332] ##Evidence-Data-END##

ATGAATAACA GAGCACATAC CTTCTTATGG GGCATCAGAC AATTCAGGAC TTCAATTCCA 
AGAAGCAGAG CTTTGAGGAC ATATTCTTTG GTATTTTGCA AACCAGAAGT AATTCATTCA 
AAACGGAACC CAAGGAACCA TCTGCTAAAT GGTTTTGATG AGGGATTGCA GCCATCTGTT 
AGATACCTCT TTCAGGATAT ATTTATTTCA AAATCAGTAG CTGGCTGTAC TCAAACTAGA 
GGCATAATCC ATGCAGCTGG TTTTAAACTT GATAGAATAC TTTGTCCTAG AAGACTGTCC 
TTTGACGCAA AACATTCTTT TGTCTCTGAT GGGACATCTG ATCATGATTT AATGAAAACA 
AACTTTCATC ATACCTCCAC TGAAGATGTG CTCACCAAGA AAATGAGACC AACTCCTGTG 
AATTATAAAA AGCTGGCCCA GGAGTGCAAC TCTCTGAGCG ATGTGCTGGA CACATTTTCA 
AAAGCGCCTA CATTTCCTGG TAGTAACTAT TTCTTAGCCA TGTGGATAAT TGCCAAAAGG 
ATTTCAGAAG ACAAGAGGCG CTTTGAGAAA CAACTGATGT TCAGTCACCC CGCCTTTAAC 
CAGCTGTGTG AGCAAATGAT GAGAGAAGCC AAGATTATGC GCTATGACCA CCTTTTGTTC 
AGTCTTAATG CTATCGTGAA GCTTGGAGTT CCTCAGAATT CTCTTATGGT ACAGACTTTG 
CTGAGGACAA TTCAGGAGCG CATCAGTGAG TGTGATGAGA GATGTCTTTC AATTTTGTCG 
ACTGCTTTAG TGACCATGGA GCCGTGCATG AATGTGAACG CGCTTCGAGC AGGGTTGCGA 
ATCCTAGTTG ATCAGCAAGT TTGGAACATA AATGATATCT TCACTTTACA AACGGTGATG 
AGATGTATTG GGAAAGATAT GAAAGCCTTG AAGGAATTAG GCAGATTTTC TGTCTTGAAT 
AGCCGGCATA TGTTTGAAGT CTTAGCTGCC ATGGATCACC GCTCTGTCGT CCTTCTGAAT 
GAGTGCAGTA AGATTGTCAT AGATAATATC CATGGGTGTC CTTTTAAAGT ATTAATCAGC 
ATACTGCAGT CCTGTAGAGA CCTCCGATAC CAAAATGAAG ATCTTTTCAA AAGCATCGCA 
GATTATGTGG CTACAACCTT TGACATCTGG AAGTTGAAAC ACGTTATCTT TTTCCTCTTG 
TCATTTGAAA CTCTTGGTTT TCGACCTCCT GGTTTGATGG ACAAGCTTCT GGAGAAAGTA 
GTCCAGGAGC CTGGCTCTCT GACCGTGAAG AACATTGTTT CTGTCCTTCA TGTGTATTCT 
TCTCTCAATC ATGTCCACAA TGTCCAGAAC AGAGAGTTCC TAGAAGCTCT GGCAAGTGCG 
CTGACTGGCT GTCTTCACCA GATCTCTTCT GAAAGCCTGT TGAATGCTGT GCATTCGTTT 
TGCATGATGA ATTATTTCCC CCTGGCCCCT ATTAACCAGC TTATCAAAGA GAACATCATC 
CACGAACTGC TGACTTCAGG TGACACAGAG AAGAATATTC ATAAGCTTCA TGTTTTGAAT 
ACTTGTCTGA AACTTGATGA AAGTACTTAC AAATGTATAC ATATACCGTT GCCACAGCTG 
CCACTGACAG CCTCACATCC AAACGAGAAG CTTGCAGAGG TGCTCAGCAG GCTTTTGGAA 
GGGGATGGAT GCTTCTCGAG AAATGTGCAG TTGCCACATA ATTATCACAT TGATTTTGAA 
ATCAGAATGG ATACTAACAG GACCCAAGTA TTTTCATTTT CTGAGGGAGA TGCAAGTTCT 
GCCACAAACA TGCAGAGAGT GGCCGTGCTG TGTGTTCCTA AGTCTGCTTA CTGTTTAAAT 
TCAAACCACC TCAGAGGATT GATGGCCATG AAAATTCGAC ATTTGAATGT AATGGGCTTC 
CATGTCATCT TGATCCATAA CTGGGAGCTG AAGAAACTAA AGATGGAGGA TGCGGTCACA 
TTTGTGAGGA AGAAGATCTA TTCAGATGAA GCACTTGCCA CTACTGACGA AAGCGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009673.1
CDS	96..2135
Translation	MNNRAHTFLWGIRQFRTSIPRSRALRTYSLVFCKPEVIHSKRNPRNHLLNGFDEGLQPSVRYLFQDIFISKSVAGCTQTRGIIHAAGFKLDRILCPRRLSFDAKHSFVSDGTSDHDLMKTNFHHTSTEDVLTKKMRPTPVNYKKLAQECNSLSDVLDTFSKAPTFPGSNYFLAMWIIAKRISEDKRRFEKQLMFSHPAFNQLCEQMMREAKIMRYDHLLFSLNAIVKLGVPQNSLMVQTLLRTIQERISECDERCLSILSTALVTMEPCMNVNALRAGLRILVDQQVWNINDIFTLQTVMRCIGKDMKALKELGRFSVLNSRHMFEVLAAMDHRSVVLLNECSKIVIDNIHGCPFKVLISILQSCRDLRYQNEDLFKSIADYVATTFDIWKLKHVIFFLLSFETLGFRPPGLMDKLLEKVVQEPGSLTVKNIVSVLHVYSSLNHVHNVQNREFLEALASALTGCLHQISSESLLNAVHSFCMMNYFPLAPINQLIKENIIHELLTSGDTEKNIHKLHVLNTCLKLDESTYKCIHIPLPQLPLTASHPNEKLAEVLSRLLEGDGCFSRNVQLPHNYHIDFEIRMDTNRTQVFSFSEGDASSATNMQRVAVLCVPKSAYCLNSNHLRGLMAMKIRHLNVMGFHVILIHNWELKKLKMEDAVTFVRKKIYSDEALATTDESV

Target ORF information:

RefSeq Version	NM_001009673.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FAST kinase domains 2 (Fastkd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009673.1

ORF Insert Sequence:

ATGAATAACA GAGCACATAC CTTCTTATGG GGCATCAGAC AATTCAGGAC TTCAATTCCA 
AGAAGCAGAG CTTTGAGGAC ATATTCTTTG GTATTTTGCA AACCAGAAGT AATTCATTCA 
AAACGGAACC CAAGGAACCA TCTGCTAAAT GGTTTTGATG AGGGATTGCA GCCATCTGTT 
AGATACCTCT TTCAGGATAT ATTTATTTCA AAATCAGTAG CTGGCTGTAC TCAAACTAGA 
GGCATAATCC ATGCAGCTGG TTTTAAACTT GATAGAATAC TTTGTCCTAG AAGACTGTCC 
TTTGACGCAA AACATTCTTT TGTCTCTGAT GGGACATCTG ATCATGATTT AATGAAAACA 
AACTTTCATC ATACCTCCAC TGAAGATGTG CTCACCAAGA AAATGAGACC AACTCCTGTG 
AATTATAAAA AGCTGGCCCA GGAGTGCAAC TCTCTGAGCG ATGTGCTGGA CACATTTTCA 
AAAGCGCCTA CATTTCCTGG TAGTAACTAT TTCTTAGCCA TGTGGATAAT TGCCAAAAGG 
ATTTCAGAAG ACAAGAGGCG CTTTGAGAAA CAACTGATGT TCAGTCACCC CGCCTTTAAC 
CAGCTGTGTG AGCAAATGAT GAGAGAAGCC AAGATTATGC GCTATGACCA CCTTTTGTTC 
AGTCTTAATG CTATCGTGAA GCTTGGAGTT CCTCAGAATT CTCTTATGGT ACAGACTTTG 
CTGAGGACAA TTCAGGAGCG CATCAGTGAG TGTGATGAGA GATGTCTTTC AATTTTGTCG 
ACTGCTTTAG TGACCATGGA GCCGTGCATG AATGTGAACG CGCTTCGAGC AGGGTTGCGA 
ATCCTAGTTG ATCAGCAAGT TTGGAACATA AATGATATCT TCACTTTACA AACGGTGATG 
AGATGTATTG GGAAAGATAT GAAAGCCTTG AAGGAATTAG GCAGATTTTC TGTCTTGAAT 
AGCCGGCATA TGTTTGAAGT CTTAGCTGCC ATGGATCACC GCTCTGTCGT CCTTCTGAAT 
GAGTGCAGTA AGATTGTCAT AGATAATATC CATGGGTGTC CTTTTAAAGT ATTAATCAGC 
ATACTGCAGT CCTGTAGAGA CCTCCGATAC CAAAATGAAG ATCTTTTCAA AAGCATCGCA 
GATTATGTGG CTACAACCTT TGACATCTGG AAGTTGAAAC ACGTTATCTT TTTCCTCTTG 
TCATTTGAAA CTCTTGGTTT TCGACCTCCT GGTTTGATGG ACAAGCTTCT GGAGAAAGTA 
GTCCAGGAGC CTGGCTCTCT GACCGTGAAG AACATTGTTT CTGTCCTTCA TGTGTATTCT 
TCTCTCAATC ATGTCCACAA TGTCCAGAAC AGAGAGTTCC TAGAAGCTCT GGCAAGTGCG 
CTGACTGGCT GTCTTCACCA GATCTCTTCT GAAAGCCTGT TGAATGCTGT GCATTCGTTT 
TGCATGATGA ATTATTTCCC CCTGGCCCCT ATTAACCAGC TTATCAAAGA GAACATCATC 
CACGAACTGC TGACTTCAGG TGACACAGAG AAGAATATTC ATAAGCTTCA TGTTTTGAAT 
ACTTGTCTGA AACTTGATGA AAGTACTTAC AAATGTATAC ATATACCGTT GCCACAGCTG 
CCACTGACAG CCTCACATCC AAACGAGAAG CTTGCAGAGG TGCTCAGCAG GCTTTTGGAA 
GGGGATGGAT GCTTCTCGAG AAATGTGCAG TTGCCACATA ATTATCACAT TGATTTTGAA 
ATCAGAATGG ATACTAACAG GACCCAAGTA TTTTCATTTT CTGAGGGAGA TGCAAGTTCT 
GCCACAAACA TGCAGAGAGT GGCCGTGCTG TGTGTTCCTA AGTCTGCTTA CTGTTTAAAT 
TCAAACCACC TCAGAGGATT GATGGCCATG AAAATTCGAC ATTTGAATGT AATGGGCTTC 
CATGTCATCT TGATCCATAA CTGGGAGCTG AAGAAACTAA AGATGGAGGA TGCGGTCACA 
TTTGTGAGGA AGAAGATCTA TTCAGATGAA GCACTTGCCA CTACTGACGA AAGCGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fastkd2 ( XM_006245050.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245112.2 Rattus norvegicus FAST kinase domains 2 (Fastkd2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62726
Clone ID Related Accession (Same CDS sequence)	XM_006245050.2
Accession Version	XM_006245050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	FAST kinase domain-containing protein 2, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAACA GAGCACATAC CTTCTTATGG GGCATCAGAC AATTCAGGAC TTCAATTCCA 
AGAAGCAGAG CTTTGAGGAC ATATTCTTTG GTATTTTGCA AACCAGAAGT AATTCATTCA 
AAACGGAACC CAAGGAACCA TCTGCTAAAT GGTTTTGATG AGGGATTGCA GCCATCTGTT 
AGATACCTCT TTCAGGATAT ATTTATTTCA AAATCAGTAG CTGGCTGTAC TCAAACTAGA 
GGCATAATCC ATGCAGCTGG TTTTAAACTT GATAGAATAC TTTGTCCTAG AAGACTGTCC 
TTTGACGCAA AACATTCTTT TGTCTCTGAT GGGACATCTG ATCATGATTT AATGAAAACA 
AACTTTCATC ATACCTCCAC TGAAGATGTG CTCACCAAGA AAATGAGACC AACTCCTGTG 
AATTATAAAA AGCTGGCCCA GGAGTGCAAC TCTCTGAGCG ATGTGCTGGA CACATTTTCA 
AAAGCGCCTA CATTTCCTGG TAGTAACTAT TTCTTAGCCA TGTGGATAAT TGCCAAAAGG 
ATTTCAGAAG ACAAGAGGCG CTTTGAGAAA CAACTGATGT TCAGTCACCC CGCCTTTAAC 
CAGCTGTGTG AGCAAATGAT GAGAGAAGCC AAGATTATGC GCTATGACCA CCTTTTGTTC 
AGTCTTAATG CTATCGTGAA GCTTGGAGTT CCTCAGAATT CTCTTATGGT ACAGACTTTG 
CTGAGGACAA TTCAGGAGCG CATCAGTGAG TGTGATGAGA GATGTCTTTC AATTTTGTCG 
ACTGCTTTAG TGACCATGGA GCCGTGCATG AATGTGAACG CGCTTCGAGC AGGGTTGCGA 
ATCCTAGTTG ATCAGCAAGT TTGGAACATA AATGATATCT TCACTTTACA AACGGTGATG 
AGATGTATTG GGAAAGATGC ACCATTTGCT CTTAAGAAGA AATTGGAGAT GAAAGCCTTG 
AAGGAATTAG GCAGATTTTC TGTCTTGAAT AGCCGGCATA TGTTTGAAGT CTTAGCTGCC 
ATGGATCACC GCTCTGTCGT CCTTCTGAAT GAGTGCAGTA AGATTGTCAT AGATAATATC 
CATGGGTGTC CTTTTAAAGT ATTAATCAGC ATACTGCAGT CCTGTAGAGA CCTCCGATAC 
CAAAATGAAG ATCTTTTCAA AAGCATCGCA GATTATGTGG CTACAACCTT TGACATCTGG 
AAGTTGAAAC ACGTTATCTT TTTCCTCTTG TCATTTGAAA CTCTTGGTTT TCGACCTCCT 
GGTTTGATGG ACAAGCTTCT GGAGAAAGTA GTCCAGGAGC CTGGCTCTCT GACCGTGAAG 
AACATTGTTT CTGTCCTTCA TGTGTATTCT TCTCTCAATC ATGTCCACAA TGTCCAGAAC 
AGAGAGTTCC TAGAAGCTCT GGCAAGTGCG CTGACTGGCT GTCTTCACCA GATCTCTTCT 
GAAAGCCTGT TGAATGCTGT GCATTCGTTT TGCATGATGA ATTATTTCCC CCTGGCCCCT 
ATTAACCAGC TTATCAAAGA GAACATCATC CACGAACTGC TGACTTCAGG TGACACAGAG 
AAGAATATTC ATAAGCTTCA TGTTTTGAAT ACTTGTCTGA AACTTGATGA AAGTACTTAC 
AAATGTATAC ATATACCGTT GCCACAGCTG CCACTGACAG CCTCACATCC AAACGAGAAG 
CTTGCAGAGG TGCTCAGCAG GCTTTTGGAA GGGGATGGAT GCTTCTCGAG AAATGTGCAG 
TTGCCACATA ATTATCACAT TGATTTTGAA ATCAGAATGG ATACTAACAG GACCCAAGTA 
TTTTCATTTT CTGAGGGAGA TGCAAGTTCT GCCACAAACA TGCAGAGAGT GGCCGTGCTG 
TGTGTTCCTA AGTCTGCTTA CTGTTTAAAT TCAAACCACC TCAGAGGATT GATGGCCATG 
AAAATTCGAC ATTTGAATGT AATGGGCTTC CATGTCATCT TGATCCATAA CTGGGAGCTG 
AAGAAACTAA AGATGGAGGA TGCGGTCACA TTTGTGAGGA AGAAGATCTA TTCAGATGAA 
GCACTTGCCA CTACTGACGA AAGCGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245112.2
CDS	192..2261
Translation	MNNRAHTFLWGIRQFRTSIPRSRALRTYSLVFCKPEVIHSKRNPRNHLLNGFDEGLQPSVRYLFQDIFISKSVAGCTQTRGIIHAAGFKLDRILCPRRLSFDAKHSFVSDGTSDHDLMKTNFHHTSTEDVLTKKMRPTPVNYKKLAQECNSLSDVLDTFSKAPTFPGSNYFLAMWIIAKRISEDKRRFEKQLMFSHPAFNQLCEQMMREAKIMRYDHLLFSLNAIVKLGVPQNSLMVQTLLRTIQERISECDERCLSILSTALVTMEPCMNVNALRAGLRILVDQQVWNINDIFTLQTVMRCIGKDAPFALKKKLEMKALKELGRFSVLNSRHMFEVLAAMDHRSVVLLNECSKIVIDNIHGCPFKVLISILQSCRDLRYQNEDLFKSIADYVATTFDIWKLKHVIFFLLSFETLGFRPPGLMDKLLEKVVQEPGSLTVKNIVSVLHVYSSLNHVHNVQNREFLEALASALTGCLHQISSESLLNAVHSFCMMNYFPLAPINQLIKENIIHELLTSGDTEKNIHKLHVLNTCLKLDESTYKCIHIPLPQLPLTASHPNEKLAEVLSRLLEGDGCFSRNVQLPHNYHIDFEIRMDTNRTQVFSFSEGDASSATNMQRVAVLCVPKSAYCLNSNHLRGLMAMKIRHLNVMGFHVILIHNWELKKLKMEDAVTFVRKKIYSDEALATTDESV

Target ORF information:

RefSeq Version	XM_006245050.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FAST kinase domains 2 (Fastkd2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245050.2

ORF Insert Sequence:

ATGAATAACA GAGCACATAC CTTCTTATGG GGCATCAGAC AATTCAGGAC TTCAATTCCA 
AGAAGCAGAG CTTTGAGGAC ATATTCTTTG GTATTTTGCA AACCAGAAGT AATTCATTCA 
AAACGGAACC CAAGGAACCA TCTGCTAAAT GGTTTTGATG AGGGATTGCA GCCATCTGTT 
AGATACCTCT TTCAGGATAT ATTTATTTCA AAATCAGTAG CTGGCTGTAC TCAAACTAGA 
GGCATAATCC ATGCAGCTGG TTTTAAACTT GATAGAATAC TTTGTCCTAG AAGACTGTCC 
TTTGACGCAA AACATTCTTT TGTCTCTGAT GGGACATCTG ATCATGATTT AATGAAAACA 
AACTTTCATC ATACCTCCAC TGAAGATGTG CTCACCAAGA AAATGAGACC AACTCCTGTG 
AATTATAAAA AGCTGGCCCA GGAGTGCAAC TCTCTGAGCG ATGTGCTGGA CACATTTTCA 
AAAGCGCCTA CATTTCCTGG TAGTAACTAT TTCTTAGCCA TGTGGATAAT TGCCAAAAGG 
ATTTCAGAAG ACAAGAGGCG CTTTGAGAAA CAACTGATGT TCAGTCACCC CGCCTTTAAC 
CAGCTGTGTG AGCAAATGAT GAGAGAAGCC AAGATTATGC GCTATGACCA CCTTTTGTTC 
AGTCTTAATG CTATCGTGAA GCTTGGAGTT CCTCAGAATT CTCTTATGGT ACAGACTTTG 
CTGAGGACAA TTCAGGAGCG CATCAGTGAG TGTGATGAGA GATGTCTTTC AATTTTGTCG 
ACTGCTTTAG TGACCATGGA GCCGTGCATG AATGTGAACG CGCTTCGAGC AGGGTTGCGA 
ATCCTAGTTG ATCAGCAAGT TTGGAACATA AATGATATCT TCACTTTACA AACGGTGATG 
AGATGTATTG GGAAAGATGC ACCATTTGCT CTTAAGAAGA AATTGGAGAT GAAAGCCTTG 
AAGGAATTAG GCAGATTTTC TGTCTTGAAT AGCCGGCATA TGTTTGAAGT CTTAGCTGCC 
ATGGATCACC GCTCTGTCGT CCTTCTGAAT GAGTGCAGTA AGATTGTCAT AGATAATATC 
CATGGGTGTC CTTTTAAAGT ATTAATCAGC ATACTGCAGT CCTGTAGAGA CCTCCGATAC 
CAAAATGAAG ATCTTTTCAA AAGCATCGCA GATTATGTGG CTACAACCTT TGACATCTGG 
AAGTTGAAAC ACGTTATCTT TTTCCTCTTG TCATTTGAAA CTCTTGGTTT TCGACCTCCT 
GGTTTGATGG ACAAGCTTCT GGAGAAAGTA GTCCAGGAGC CTGGCTCTCT GACCGTGAAG 
AACATTGTTT CTGTCCTTCA TGTGTATTCT TCTCTCAATC ATGTCCACAA TGTCCAGAAC 
AGAGAGTTCC TAGAAGCTCT GGCAAGTGCG CTGACTGGCT GTCTTCACCA GATCTCTTCT 
GAAAGCCTGT TGAATGCTGT GCATTCGTTT TGCATGATGA ATTATTTCCC CCTGGCCCCT 
ATTAACCAGC TTATCAAAGA GAACATCATC CACGAACTGC TGACTTCAGG TGACACAGAG 
AAGAATATTC ATAAGCTTCA TGTTTTGAAT ACTTGTCTGA AACTTGATGA AAGTACTTAC 
AAATGTATAC ATATACCGTT GCCACAGCTG CCACTGACAG CCTCACATCC AAACGAGAAG 
CTTGCAGAGG TGCTCAGCAG GCTTTTGGAA GGGGATGGAT GCTTCTCGAG AAATGTGCAG 
TTGCCACATA ATTATCACAT TGATTTTGAA ATCAGAATGG ATACTAACAG GACCCAAGTA 
TTTTCATTTT CTGAGGGAGA TGCAAGTTCT GCCACAAACA TGCAGAGAGT GGCCGTGCTG 
TGTGTTCCTA AGTCTGCTTA CTGTTTAAAT TCAAACCACC TCAGAGGATT GATGGCCATG 
AAAATTCGAC ATTTGAATGT AATGGGCTTC CATGTCATCT TGATCCATAA CTGGGAGCTG 
AAGAAACTAA AGATGGAGGA TGCGGTCACA TTTGTGAGGA AGAAGATCTA TTCAGATGAA 
GCACTTGCCA CTACTGACGA AAGCGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.
Cell metabolism24(6)875-885(2016 Dec)
Arroyo JD,Jourdain AA,Calvo SE,Ballarano CA,Doench JG,Root DE,Mootha VK

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.
Cell reports(2015 Feb)
Antonicka H,Shoubridge EA

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Fast kinase domain-containing protein 3 is a mitochondrial protein essential for cellular respiration.
Biochemical and biophysical research communications401(3)440-6(2010 Oct)
Simarro M,Gimenez-Cassina A,Kedersha N,Lazaro JB,Adelmant GO,Marto JA,Rhee K,Tisdale S,Danial N,Benarafa C,Ordu?a A,Anderson P

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Fastkd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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