Zap70 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zap70
Entrez Gene ID	301348
Full Name	zeta chain of T cell receptor associated protein kinase 70
Synonyms	Srk
General protein information	Preferred Names zeta chain of T cell receptor associated protein kinase 70 Names tyrosine-protein kinase ZAP-70 syk-related protein tyrosine kinase zeta-chain (TCR) associated protein kinase 70
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_006244761.3	XP_006244823.1	tyrosine-protein kinase ZAP-70 isoform X1
NM_001012002.1	NP_001012002.1	tyrosine-protein kinase ZAP-70
XM_008766998.2	XP_008765220.1	tyrosine-protein kinase ZAP-70 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZAP70	NP_001070.2
Canis lupus familiaris (dog)	ZAP70	XP_005626103.1
Gallus gallus (chicken)	ZAP70	XP_418206.4
Xenopus tropicalis (tropical clawed frog)	zap70	NP_001006825.1
	ZAP70	XP_001101570.2
Pan troglodytes (chimpanzee)	ZAP70	XP_515637.2
Mus musculus (house mouse)	Zap70	NP_033565.2
Rattus norvegicus (Norway rat)	Zap70	NP_001012002.1
Danio rerio (zebrafish)	zap70	NP_001018425.1
Bos taurus (cattle)	ZAP70	NP_001179946.1

Gene Ontology
Bibliography

Related articles in PubMed

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Liang Y, Cucchetti M, Roncagalli R, Yokosuka T, Malzac A, Bertosio E, Imbert J, Nijman IJ, Suchanek M, Saito T, W?lfing C, Malissen B, Malissen M
Nature immunology14(8)858-66(2013 Aug)

CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion and migration.
Chiang YJ, Ho KC, Sun CT, Chiu JJ, Lee FJ, Liao F, Yang-Yen HF, Yen JJ
PloS one8(4)e61761(2013)

Interchangeability of Themis1 and Themis2 in thymocyte development reveals two related proteins with conserved molecular function.
Lesourne R, Zvezdova E, Song KD, El-Khoury D, Uehara S, Barr VA, Samelson LE, Love PE
Journal of immunology (Baltimore, Md. : 1950)189(3)1154-61(2012 Aug)

Syk kinase is phosphorylated in specific areas of the developing nervous system.
Hatterer E, Benon A, Chounlamountri N, Watrin C, Angibaud J, Jouanneau E, Boudin H, Honnorat J, Pellier-Monnin V, Noraz N
Neuroscience research70(2)172-82(2011 Jun)

RhoH GTPase recruits and activates Zap70 required for T cell receptor signaling and thymocyte development.
Gu Y, Chae HD, Siefring JE, Jasti AC, Hildeman DA, Williams DA
Nature immunology7(11)1182-90(2006 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ZAP-70 is present in neurons of different structures including the cerebellum, the hippocampus, the visual system and the olfactory system.
Title: Syk kinase is phosphorylated in specific areas of the developing nervous system.

The following Zap70 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zap70 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01919	NM_001012002.1 Latest version!	Rattus norvegicus zeta chain of T cell receptor associated protein kinase 70 (Zap70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa32116	XM_006244761.3 Latest version!	Rattus norvegicus zeta-chain (TCR) associated protein kinase 70 (Zap70), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa45832	XM_008766998.2 Latest version!	Rattus norvegicus zeta-chain (TCR) associated protein kinase 70 (Zap70), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zap70 ( NM_001012002.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012002.1 Rattus norvegicus zeta chain of T cell receptor associated protein kinase 70 (Zap70), mRNA.

Price & Availability↑

CloneID	ORa01919
Clone ID Related Accession (Same CDS sequence)	NM_001012002.1
Accession Version	NM_001012002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein kinase ZAP-70
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC089855.1. On Feb 11, 2005 this sequence version replaced XM_217382.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC089855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689605 [ECO:0000348] ##Evidence-Data-END##

ATGCCCGACC CCGCGGCGCA TCTGCCATTC TTCTACGGAA GCATTTCGCG GGCCGAGGCC 
GAGGAGCACC TGAAGCTGGC AGGCATGGCC GACGGACTGT TCCTCCTGCG CCAGTGCCTG 
CGCTCGCTGG GCGGCTACGT GCTGTCGCTA GTACACGACG TGCGCTTCCA CCATTTCCCC 
ATCGAGCGCC AACTCAATGG CACGTACGCC ATCGCGGGCG GCAAGGCGCA CTGCGGCCCG 
GCCGAGCTCT GCCAGTTCTA CTCGCAGGAC CCCGACGGAC TGCCCTGCAA TCTGCGTAAG 
CCATGCAACC GGCCGCCGGG ACTTGAGCCT CAGCCCGGGG TCTTCGACTG CCTGCGCGAT 
GCTATGGTGC GGGACTACGT GCGCCAGACC TGGAAACTGG AGGGTGATGC ACTAGAGCAG 
GCCATCATCA GCCAGGCCCC ACAGGTGGAG AAGCTCATTG CTACCACAGC CCACGAGCGA 
ATGCCCTGGT ACCACAGCAG CCTGACTCGC GAGGAGGCTG AGCGCAAACT CTATTCTGGC 
CAGCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAGG AGCAGGGCAC ATATGCGCTG 
TCCCTGGTCT ACGGGAAAAC TGTGTACCAC TATCTCATCA GCCAGGACAA GGCTGGCAAG 
TACTGTATCC CCGAAGGCAC CAAGTTTGAT ACGCTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCAG ATGGACTCAT TTATCGCCTG AAGGAGGTCT GTCCCAACAG CAGTGCCAGC 
GCCGAAGCTG CTGCACCCAC GCTCCCAGCC CACCCATCCA CATTCACTCA GCCTCACAGA 
CGAATTGATA CCCTGAACTC AGACGGATAC ACCCCTGAAC CAGCACGCTT AGACAAGCCA 
CGACCGATGC CCATGGACAC GAGCGTGTAC GAGAGCCCCT ACAGCGACCC CGAGGAACTC 
AAGGACAAGA AGCTCTTCCT GAAGCGAGAG AACCTCCTCG TGGCCGACAT CGAACTTGGT 
TGCGGCAACT TTGGCTCAGT GCGCCAGGGA GTCTACCGCA TGCGCAAGAA GCAGATCGAC 
GTGGCCATCA AGGTGCTAAA GCAGAGCACA GAGAAAGCTG ACAAGGATGA GATGATGCGG 
GAGGCCCAGA TCATGCATCA GCTCGACAAC CCCTACATTG TGCGGCTCAT CGGCGTGTGC 
CAGGCAGAGG CGCTCATGCT GGTCATGGAG ATGGCAGGAG GCGGGCCACT GCACAAGTTC 
CTGATTGGAA AAAAGGAGGA GATCCCGGTG AGCAACGTGG CTGAACTGCT GCACCAGGTG 
GCCATGGGCA TGAAGTATTT GGAGGAGAAG AACTTTGTGC ACCGTGACCT GGCAGCCCGG 
AATGTTCTAC TGGTCAATCG GCACTATGCC AAGATCAGCG ACTTTGGCCT GTCCAAGGCA 
CTGGGTGCTG ATGACAGCTA TTACACAGCC CGTTCTGCAG GGAAGTGGCC TCTGAAGTGG 
TACGCACCAG AATGCATCAA CTTCCGGAAG TTCTCCAGCC GCAGTGATGT CTGGAGCTAT 
GGGGTCACCA TGTGGGAGGC CTTCTCCTAT GGCCAGAAGC CCTACAAGAA GATGAAGGGC 
CCTGAGGTCC TGGATTTCAT CAAGCAGGGT AAGAGGATGG AATGCCCTCC AGAGTGTCCG 
CCCGAAATGT ACGCGCTTAT GAGTGACTGC TGGATATACA AGTGGGAGGA TCGTCCCGAT 
TTCGTGGCTG TGGAACAACG AATGAGGACC TATTACTACA GCATGGCTAG CCGGGCAGAG 
GGACCCCCAC AGTGTGAACA GGTGGCCGAG GCTGCATGTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012002.1
CDS	109..1953
Translation	MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCQFYSQDPDGLPCNLRKPCNRPPGLEPQPGVFDCLRDAMVRDYVRQTWKLEGDALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGQQTDGKFLLRPRKEQGTYALSLVYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYRLKEVCPNSSASAEAAAPTLPAHPSTFTQPHRRIDTLNSDGYTPEPARLDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQSTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLIGKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVLDFIKQGKRMECPPECPPEMYALMSDCWIYKWEDRPDFVAVEQRMRTYYYSMASRAEGPPQCEQVAEAACG

Target ORF information:

RefSeq Version	NM_001012002.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus zeta chain of T cell receptor associated protein kinase 70 (Zap70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012002.1

ORF Insert Sequence:

ATGCCCGACC CCGCGGCGCA TCTGCCATTC TTCTACGGAA GCATTTCGCG GGCCGAGGCC 
GAGGAGCACC TGAAGCTGGC AGGCATGGCC GACGGACTGT TCCTCCTGCG CCAGTGCCTG 
CGCTCGCTGG GCGGCTACGT GCTGTCGCTA GTACACGACG TGCGCTTCCA CCATTTCCCC 
ATCGAGCGCC AACTCAATGG CACGTACGCC ATCGCGGGCG GCAAGGCGCA CTGCGGCCCG 
GCCGAGCTCT GCCAGTTCTA CTCGCAGGAC CCCGACGGAC TGCCCTGCAA TCTGCGTAAG 
CCATGCAACC GGCCGCCGGG ACTTGAGCCT CAGCCCGGGG TCTTCGACTG CCTGCGCGAT 
GCTATGGTGC GGGACTACGT GCGCCAGACC TGGAAACTGG AGGGTGATGC ACTAGAGCAG 
GCCATCATCA GCCAGGCCCC ACAGGTGGAG AAGCTCATTG CTACCACAGC CCACGAGCGA 
ATGCCCTGGT ACCACAGCAG CCTGACTCGC GAGGAGGCTG AGCGCAAACT CTATTCTGGC 
CAGCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAGG AGCAGGGCAC ATATGCGCTG 
TCCCTGGTCT ACGGGAAAAC TGTGTACCAC TATCTCATCA GCCAGGACAA GGCTGGCAAG 
TACTGTATCC CCGAAGGCAC CAAGTTTGAT ACGCTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCAG ATGGACTCAT TTATCGCCTG AAGGAGGTCT GTCCCAACAG CAGTGCCAGC 
GCCGAAGCTG CTGCACCCAC GCTCCCAGCC CACCCATCCA CATTCACTCA GCCTCACAGA 
CGAATTGATA CCCTGAACTC AGACGGATAC ACCCCTGAAC CAGCACGCTT AGACAAGCCA 
CGACCGATGC CCATGGACAC GAGCGTGTAC GAGAGCCCCT ACAGCGACCC CGAGGAACTC 
AAGGACAAGA AGCTCTTCCT GAAGCGAGAG AACCTCCTCG TGGCCGACAT CGAACTTGGT 
TGCGGCAACT TTGGCTCAGT GCGCCAGGGA GTCTACCGCA TGCGCAAGAA GCAGATCGAC 
GTGGCCATCA AGGTGCTAAA GCAGAGCACA GAGAAAGCTG ACAAGGATGA GATGATGCGG 
GAGGCCCAGA TCATGCATCA GCTCGACAAC CCCTACATTG TGCGGCTCAT CGGCGTGTGC 
CAGGCAGAGG CGCTCATGCT GGTCATGGAG ATGGCAGGAG GCGGGCCACT GCACAAGTTC 
CTGATTGGAA AAAAGGAGGA GATCCCGGTG AGCAACGTGG CTGAACTGCT GCACCAGGTG 
GCCATGGGCA TGAAGTATTT GGAGGAGAAG AACTTTGTGC ACCGTGACCT GGCAGCCCGG 
AATGTTCTAC TGGTCAATCG GCACTATGCC AAGATCAGCG ACTTTGGCCT GTCCAAGGCA 
CTGGGTGCTG ATGACAGCTA TTACACAGCC CGTTCTGCAG GGAAGTGGCC TCTGAAGTGG 
TACGCACCAG AATGCATCAA CTTCCGGAAG TTCTCCAGCC GCAGTGATGT CTGGAGCTAT 
GGGGTCACCA TGTGGGAGGC CTTCTCCTAT GGCCAGAAGC CCTACAAGAA GATGAAGGGC 
CCTGAGGTCC TGGATTTCAT CAAGCAGGGT AAGAGGATGG AATGCCCTCC AGAGTGTCCG 
CCCGAAATGT ACGCGCTTAT GAGTGACTGC TGGATATACA AGTGGGAGGA TCGTCCCGAT 
TTCGTGGCTG TGGAACAACG AATGAGGACC TATTACTACA GCATGGCTAG CCGGGCAGAG 
GGACCCCCAC AGTGTGAACA GGTGGCCGAG GCTGCATGTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zap70 ( XM_006244761.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244823.1 Rattus norvegicus zeta-chain (TCR) associated protein kinase 70 (Zap70), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32116
Clone ID Related Accession (Same CDS sequence)	XM_006244761.3
Accession Version	XM_006244761.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein kinase ZAP-70 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244761.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAATCT CTCCTGGAAA ACCAGGGAGG AGCCTGTTGC CTTCTATGGC ATGCTTAGTA 
CAGGGCCCAG CGATGCCCGA CCCCGCGGCG CATCTGCCAT TCTTCTACGG AAGCATCTCG 
CGGGCCGAGG CCGAGGAGCA CCTGAAGCTG GCAGGCATGG CCGACGGACT GTTCCTCCTG 
CGCCAGTGCC TGCGCTCGCT GGGCGGCTAC GTGCTGTCGC TAGTACACGA CGTGCGCTTC 
CACCATTTCC CCATCGAGCG CCAACTCAAT GGCACGTACG CCATCGCGGG CGGCAAGGCG 
CACTGCGGCC CGGCCGAGCT CTGCCAGTTC TACTCGCAGG ACCCCGACGG ACTGCCCTGC 
AATCTGCGTA AGCCATGCAA CCGGCCGCCG GGACTTGAGC CTCAGCCCGG GGTCTTCGAC 
TGCCTGCGCG ATGCTATGGT GCGGGACTAC GTGCGCCAGA CCTGGAAACT GGAGGGTGAT 
GCACTAGAGC AGGCCATCAT CAGCCAGGCC CCACAGGTGG AGAAGCTCAT TGCTACCACA 
GCCCACGAGC GAATGCCCTG GTACCACAGC AGCCTGACTC GCGAGGAGGC TGAGCGCAAA 
CTCTATTCTG GCCAGCAGAC CGACGGCAAG TTCCTGCTGA GACCCCGGAA GGAGCAGGGC 
ACATATGCGC TGTCCCTGGT CTACGGGAAA ACTGTGTACC ACTATCTCAT CAGCCAGGAC 
AAGGCTGGCA AGTACTGTAT CCCCGAAGGC ACCAAGTTTG ATACGCTCTG GCAGCTGGTG 
GAGTACCTGA AGCTGAAGGC AGATGGACTC ATTTATCGCC TGAAGGAGGT CTGTCCCAAC 
AGCAGTGCCA GCGCCGAAGC TGCTGCACCC ACGCTCCCAG CCCACCCATC CACATTCACT 
CAGCCTCACA GACGAATTGA TACCCTGAAC TCAGACGGAT ACACCCCTGA ACCAGCACGC 
TTAGACAAGC CACGACCGAT GCCCATGGAC ACGAGCGTGT ACGAGAGCCC CTACAGCGAC 
CCCGAGGAAC TCAAGGACAA GAAGCTCTTC CTGAAGCGAG AGAACCTCCT CGTGGCCGAC 
ATCGAACTTG GTTGCGGCAA CTTTGGCTCA GTGCGCCAGG GAGTCTACCG CATGCGCAAG 
AAGCAGATCG ACGTGGCCAT CAAGGTGCTA AAGCAGAGCA CAGAGAAAGC TGACAAGGAT 
GAGATGATGC GGGAGGCCCA GATCATGCAT CAGCTCGACA ACCCCTACAT TGTGCGGCTC 
ATCGGCGTGT GCCAGGCAGA GGCGCTCATG CTGGTCATGG AGATGGCAGG AGGCGGGCCA 
CTGCACAAGT TCCTGATTGG AAAAAAGGAG GAGATCCCGG TGAGCAACGT GGCTGAACTG 
CTGCACCAGG TGGCCATGGG CATGAAGTAT TTGGAGGAGA AGAACTTTGT GCACCGTGAC 
CTGGCAGCCC GGAATGTTCT ACTGGTCAAT CGGCACTATG CCAAGATCAG CGACTTTGGC 
CTGTCCAAGG CACTGGGTGC TGATGACAGC TATTACACAG CCCGTTCTGC AGGGAAGTGG 
CCTCTGAAGT GGTACGCACC AGAATGCATC AACTTCCGGA AGTTCTCCAG CCGCAGTGAT 
GTCTGGAGCT ATGGGGTCAC CATGTGGGAG GCCTTCTCCT ATGGCCAGAA GCCCTACAAG 
AAGATGAAGG GCCCTGAGGT CCTGGATTTC ATCAAGCAGG GTAAGAGGAT GGAATGCCCT 
CCAGAGTGTC CGCCCGAAAT GTACGCGCTT ATGAGTGACT GCTGGATATA CAAGTGGGAG 
GATCGTCCCG ATTTCGTGGC TGTGGAACAA CGAATGAGGA CCTATTACTA CAGCATGGCT 
AGCCGGGCAG AGGGACCCCC ACAGTGTGAA CAGGTGGCCG AGGCTGCATG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244823.1
CDS	1175..3091
Translation	MQISPGKPGRSLLPSMACLVQGPAMPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCQFYSQDPDGLPCNLRKPCNRPPGLEPQPGVFDCLRDAMVRDYVRQTWKLEGDALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGQQTDGKFLLRPRKEQGTYALSLVYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYRLKEVCPNSSASAEAAAPTLPAHPSTFTQPHRRIDTLNSDGYTPEPARLDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQSTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLIGKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVLDFIKQGKRMECPPECPPEMYALMSDCWIYKWEDRPDFVAVEQRMRTYYYSMASRAEGPPQCEQVAEAACG

Target ORF information:

RefSeq Version	XM_006244761.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus zeta-chain (TCR) associated protein kinase 70 (Zap70), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244761.3

ORF Insert Sequence:

ATGCAAATCT CTCCTGGAAA ACCAGGGAGG AGCCTGTTGC CTTCTATGGC ATGCTTAGTA 
CAGGGCCCAG CGATGCCCGA CCCCGCGGCG CATCTGCCAT TCTTCTACGG AAGCATCTCG 
CGGGCCGAGG CCGAGGAGCA CCTGAAGCTG GCAGGCATGG CCGACGGACT GTTCCTCCTG 
CGCCAGTGCC TGCGCTCGCT GGGCGGCTAC GTGCTGTCGC TAGTACACGA CGTGCGCTTC 
CACCATTTCC CCATCGAGCG CCAACTCAAT GGCACGTACG CCATCGCGGG CGGCAAGGCG 
CACTGCGGCC CGGCCGAGCT CTGCCAGTTC TACTCGCAGG ACCCCGACGG ACTGCCCTGC 
AATCTGCGTA AGCCATGCAA CCGGCCGCCG GGACTTGAGC CTCAGCCCGG GGTCTTCGAC 
TGCCTGCGCG ATGCTATGGT GCGGGACTAC GTGCGCCAGA CCTGGAAACT GGAGGGTGAT 
GCACTAGAGC AGGCCATCAT CAGCCAGGCC CCACAGGTGG AGAAGCTCAT TGCTACCACA 
GCCCACGAGC GAATGCCCTG GTACCACAGC AGCCTGACTC GCGAGGAGGC TGAGCGCAAA 
CTCTATTCTG GCCAGCAGAC CGACGGCAAG TTCCTGCTGA GACCCCGGAA GGAGCAGGGC 
ACATATGCGC TGTCCCTGGT CTACGGGAAA ACTGTGTACC ACTATCTCAT CAGCCAGGAC 
AAGGCTGGCA AGTACTGTAT CCCCGAAGGC ACCAAGTTTG ATACGCTCTG GCAGCTGGTG 
GAGTACCTGA AGCTGAAGGC AGATGGACTC ATTTATCGCC TGAAGGAGGT CTGTCCCAAC 
AGCAGTGCCA GCGCCGAAGC TGCTGCACCC ACGCTCCCAG CCCACCCATC CACATTCACT 
CAGCCTCACA GACGAATTGA TACCCTGAAC TCAGACGGAT ACACCCCTGA ACCAGCACGC 
TTAGACAAGC CACGACCGAT GCCCATGGAC ACGAGCGTGT ACGAGAGCCC CTACAGCGAC 
CCCGAGGAAC TCAAGGACAA GAAGCTCTTC CTGAAGCGAG AGAACCTCCT CGTGGCCGAC 
ATCGAACTTG GTTGCGGCAA CTTTGGCTCA GTGCGCCAGG GAGTCTACCG CATGCGCAAG 
AAGCAGATCG ACGTGGCCAT CAAGGTGCTA AAGCAGAGCA CAGAGAAAGC TGACAAGGAT 
GAGATGATGC GGGAGGCCCA GATCATGCAT CAGCTCGACA ACCCCTACAT TGTGCGGCTC 
ATCGGCGTGT GCCAGGCAGA GGCGCTCATG CTGGTCATGG AGATGGCAGG AGGCGGGCCA 
CTGCACAAGT TCCTGATTGG AAAAAAGGAG GAGATCCCGG TGAGCAACGT GGCTGAACTG 
CTGCACCAGG TGGCCATGGG CATGAAGTAT TTGGAGGAGA AGAACTTTGT GCACCGTGAC 
CTGGCAGCCC GGAATGTTCT ACTGGTCAAT CGGCACTATG CCAAGATCAG CGACTTTGGC 
CTGTCCAAGG CACTGGGTGC TGATGACAGC TATTACACAG CCCGTTCTGC AGGGAAGTGG 
CCTCTGAAGT GGTACGCACC AGAATGCATC AACTTCCGGA AGTTCTCCAG CCGCAGTGAT 
GTCTGGAGCT ATGGGGTCAC CATGTGGGAG GCCTTCTCCT ATGGCCAGAA GCCCTACAAG 
AAGATGAAGG GCCCTGAGGT CCTGGATTTC ATCAAGCAGG GTAAGAGGAT GGAATGCCCT 
CCAGAGTGTC CGCCCGAAAT GTACGCGCTT ATGAGTGACT GCTGGATATA CAAGTGGGAG 
GATCGTCCCG ATTTCGTGGC TGTGGAACAA CGAATGAGGA CCTATTACTA CAGCATGGCT 
AGCCGGGCAG AGGGACCCCC ACAGTGTGAA CAGGTGGCCG AGGCTGCATG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zap70 ( XM_008766998.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765220.1 Rattus norvegicus zeta-chain (TCR) associated protein kinase 70 (Zap70), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa45832
Clone ID Related Accession (Same CDS sequence)	XM_008766998.2
Accession Version	XM_008766998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein kinase ZAP-70 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACC CCGCGGCGCA TCTGCCATTC TTCTACGGAA GCATCTCGCG GGCCGAGGCC 
GAGGAGCACC TGAAGCTGGC AGGCATGGCC GACGGACTGT TCCTCCTGCG CCAGTGCCTG 
CGCTCGCTGG GCGGCTACGT GCTGTCGCTA GTACACGACG TGCGCTTCCA CCATTTCCCC 
ATCGAGCGCC AACTCAATGG CACGTACGCC ATCGCGGGCG GCAAGGCGCA CTGCGGCCCG 
GCCGAGCTCT GCCAGTTCTA CTCGCAGGAC CCCGACGGAC TGCCCTGCAA TCTGCGTAAG 
CCATGCAACC GGCCGCCGGG ACTTGAGCCT CAGCCCGGGG TCTTCGACTG CCTGCGCGAT 
GCTATGGTGC GGGACTACGT GCGCCAGACC TGGAAACTGG AGGGTGATGC ACTAGAGCAG 
GCCATCATCA GCCAGGCCCC ACAGGTGGAG AAGCTCATTG CTACCACAGC CCACGAGCGA 
ATGCCCTGGT ACCACAGCAG CCTGACTCGC GAGGAGGCTG AGCGCAAACT CTATTCTGGC 
CAGCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAGG AGCAGGGCAC ATATGCGCTG 
TCCCTGGTCT ACGGGAAAAC TGTGTACCAC TATCTCATCA GCCAGGACAA GGCTGGCAAG 
TACTGTATCC CCGAAGGCAC CAAGTTTGAT ACGCTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCAG ATGGACTCAT TTATCGCCTG AAGGAGGTCT GTCCCAACAG CAGTGCCAGC 
GCCGAAGCTG CTGCACCCAC GCTCCCAGCC CACCCATCCA CATTCACTCA GCCTCACAGA 
CGAATTGATA CCCTGAACTC AGACGGATAC ACCCCTGAAC CAGCACGCTT AGACAAGCCA 
CGACCGATGC CCATGGACAC GAGCGTGTAC GAGAGCCCCT ACAGCGACCC CGAGGAACTC 
AAGGACAAGA AGCTCTTCCT GAAGCGAGAG AACCTCCTCG TGGCCGACAT CGAACTTGGT 
TGCGGCAACT TTGGCTCAGT GCGCCAGGGA GTCTACCGCA TGCGCAAGAA GCAGATCGAC 
GTGGCCATCA AGGTGCTAAA GCAGAGCACA GAGAAAGCTG ACAAGGATGA GATGATGCGG 
GAGGCCCAGA TCATGCATCA GCTCGACAAC CCCTACATTG TGCGGCTCAT CGGCGTGTGC 
CAGGCAGAGG CGCTCATGCT GGTCATGGAG ATGGCAGGAG GCGGGCCACT GCACAAGTTC 
CTGATTGGAA AAAAGGAGGA GATCCCGGTG AGCAACGTGG CTGAACTGCT GCACCAGGTG 
GCCATGGGCA TGAAGTATTT GGAGGAGAAG AACTTTGTGC ACCGTGACCT GGCAGCCCGG 
AATGTTCTAC TGGTCAATCG GCACTATGCC AAGATCAGCG ACTTTGGCCT GTCCAAGGCA 
CTGGGTGCTG ATGACAGCTA TTACACAGCC CGTTCTGCAG GGAAGTGGCC TCTGAAGTGG 
TACGCACCAG AATGCATCAA CTTCCGGAAG TTCTCCAGCC GCAGTGATGT CTGGAGCTAT 
GGGGTCACCA TGTGGGAGGC CTTCTCCTAT GGCCAGAAGC CCTACAAGAA GATGAAGGGC 
CCTGAGGTCC TGGATTTCAT CAAGCAGGGT AAGAGGATGG AATGCCCTCC AGAGTGTCCG 
CCCGAAATGT ACGCGCTTAT GAGTGACTGC TGGATATACA AGTGGGAGGA TCGTCCCGAT 
TTCGTGGCTG TGGAACAACG AATGAGGACC TATTACTACA GCATGGCTAG CCGGGCAGAG 
GGACCCCCAC AGTGTGAACA GGTGGCCGAG GCTGCATGTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765220.1
CDS	940..2784
Translation	MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCQFYSQDPDGLPCNLRKPCNRPPGLEPQPGVFDCLRDAMVRDYVRQTWKLEGDALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGQQTDGKFLLRPRKEQGTYALSLVYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYRLKEVCPNSSASAEAAAPTLPAHPSTFTQPHRRIDTLNSDGYTPEPARLDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQSTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLIGKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVLDFIKQGKRMECPPECPPEMYALMSDCWIYKWEDRPDFVAVEQRMRTYYYSMASRAEGPPQCEQVAEAACG

Target ORF information:

RefSeq Version	XM_008766998.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus zeta-chain (TCR) associated protein kinase 70 (Zap70), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766998.2

ORF Insert Sequence:

ATGCCCGACC CCGCGGCGCA TCTGCCATTC TTCTACGGAA GCATCTCGCG GGCCGAGGCC 
GAGGAGCACC TGAAGCTGGC AGGCATGGCC GACGGACTGT TCCTCCTGCG CCAGTGCCTG 
CGCTCGCTGG GCGGCTACGT GCTGTCGCTA GTACACGACG TGCGCTTCCA CCATTTCCCC 
ATCGAGCGCC AACTCAATGG CACGTACGCC ATCGCGGGCG GCAAGGCGCA CTGCGGCCCG 
GCCGAGCTCT GCCAGTTCTA CTCGCAGGAC CCCGACGGAC TGCCCTGCAA TCTGCGTAAG 
CCATGCAACC GGCCGCCGGG ACTTGAGCCT CAGCCCGGGG TCTTCGACTG CCTGCGCGAT 
GCTATGGTGC GGGACTACGT GCGCCAGACC TGGAAACTGG AGGGTGATGC ACTAGAGCAG 
GCCATCATCA GCCAGGCCCC ACAGGTGGAG AAGCTCATTG CTACCACAGC CCACGAGCGA 
ATGCCCTGGT ACCACAGCAG CCTGACTCGC GAGGAGGCTG AGCGCAAACT CTATTCTGGC 
CAGCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAGG AGCAGGGCAC ATATGCGCTG 
TCCCTGGTCT ACGGGAAAAC TGTGTACCAC TATCTCATCA GCCAGGACAA GGCTGGCAAG 
TACTGTATCC CCGAAGGCAC CAAGTTTGAT ACGCTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCAG ATGGACTCAT TTATCGCCTG AAGGAGGTCT GTCCCAACAG CAGTGCCAGC 
GCCGAAGCTG CTGCACCCAC GCTCCCAGCC CACCCATCCA CATTCACTCA GCCTCACAGA 
CGAATTGATA CCCTGAACTC AGACGGATAC ACCCCTGAAC CAGCACGCTT AGACAAGCCA 
CGACCGATGC CCATGGACAC GAGCGTGTAC GAGAGCCCCT ACAGCGACCC CGAGGAACTC 
AAGGACAAGA AGCTCTTCCT GAAGCGAGAG AACCTCCTCG TGGCCGACAT CGAACTTGGT 
TGCGGCAACT TTGGCTCAGT GCGCCAGGGA GTCTACCGCA TGCGCAAGAA GCAGATCGAC 
GTGGCCATCA AGGTGCTAAA GCAGAGCACA GAGAAAGCTG ACAAGGATGA GATGATGCGG 
GAGGCCCAGA TCATGCATCA GCTCGACAAC CCCTACATTG TGCGGCTCAT CGGCGTGTGC 
CAGGCAGAGG CGCTCATGCT GGTCATGGAG ATGGCAGGAG GCGGGCCACT GCACAAGTTC 
CTGATTGGAA AAAAGGAGGA GATCCCGGTG AGCAACGTGG CTGAACTGCT GCACCAGGTG 
GCCATGGGCA TGAAGTATTT GGAGGAGAAG AACTTTGTGC ACCGTGACCT GGCAGCCCGG 
AATGTTCTAC TGGTCAATCG GCACTATGCC AAGATCAGCG ACTTTGGCCT GTCCAAGGCA 
CTGGGTGCTG ATGACAGCTA TTACACAGCC CGTTCTGCAG GGAAGTGGCC TCTGAAGTGG 
TACGCACCAG AATGCATCAA CTTCCGGAAG TTCTCCAGCC GCAGTGATGT CTGGAGCTAT 
GGGGTCACCA TGTGGGAGGC CTTCTCCTAT GGCCAGAAGC CCTACAAGAA GATGAAGGGC 
CCTGAGGTCC TGGATTTCAT CAAGCAGGGT AAGAGGATGG AATGCCCTCC AGAGTGTCCG 
CCCGAAATGT ACGCGCTTAT GAGTGACTGC TGGATATACA AGTGGGAGGA TCGTCCCGAT 
TTCGTGGCTG TGGAACAACG AATGAGGACC TATTACTACA GCATGGCTAG CCGGGCAGAG 
GGACCCCCAC AGTGTGAACA GGTGGCCGAG GCTGCATGTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Nature immunology14(8)858-66(2013 Aug)
Liang Y,Cucchetti M,Roncagalli R,Yokosuka T,Malzac A,Bertosio E,Imbert J,Nijman IJ,Suchanek M,Saito T,W?lfing C,Malissen B,Malissen M

CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion and migration.
PloS one8(4)e61761(2013)
Chiang YJ,Ho KC,Sun CT,Chiu JJ,Lee FJ,Liao F,Yang-Yen HF,Yen JJ

Interchangeability of Themis1 and Themis2 in thymocyte development reveals two related proteins with conserved molecular function.
Journal of immunology (Baltimore, Md. : 1950)189(3)1154-61(2012 Aug)
Lesourne R,Zvezdova E,Song KD,El-Khoury D,Uehara S,Barr VA,Samelson LE,Love PE

Syk kinase is phosphorylated in specific areas of the developing nervous system.
Neuroscience research70(2)172-82(2011 Jun)
Hatterer E,Benon A,Chounlamountri N,Watrin C,Angibaud J,Jouanneau E,Boudin H,Honnorat J,Pellier-Monnin V,Noraz N

RhoH GTPase recruits and activates Zap70 required for T cell receptor signaling and thymocyte development.
Nature immunology7(11)1182-90(2006 Nov)
Gu Y,Chae HD,Siefring JE,Jasti AC,Hildeman DA,Williams DA

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Zap70 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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