Ube2j2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2j2
Entrez Gene ID	298689
Full Name	ubiquitin-conjugating enzyme E2, J2
General protein information	Preferred Names ubiquitin-conjugating enzyme E2, J2 Names ubiquitin-conjugating enzyme E2 J2 ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764354.2	XP_008762576.1	ubiquitin-conjugating enzyme E2 J2 isoform X1
XM_008764355.2	XP_008762577.1	ubiquitin-conjugating enzyme E2 J2 isoform X2
XM_006239558.3	XP_006239620.1	ubiquitin-conjugating enzyme E2 J2 isoform X2
XM_006239554.3	XP_006239616.1	ubiquitin-conjugating enzyme E2 J2 isoform X1
XM_006239555.3	XP_006239617.1	ubiquitin-conjugating enzyme E2 J2 isoform X1
XM_006239557.3	XP_006239619.1	ubiquitin-conjugating enzyme E2 J2 isoform X2
NM_001007655.2	NP_001007656.2	ubiquitin-conjugating enzyme E2 J2

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE2J2	NP_477515.2
	UBE2J2	XP_001092754.2
Kluyveromyces lactis	KLLA0E20395g	XP_454873.1
Arabidopsis thaliana (thale cress)	UBC33	NP_001032048.2
Oryza sativa (rice)	Os06g0193000	NP_001057040.1
Bos taurus (cattle)	UBE2J2	NP_001033132.1
Gallus gallus (chicken)	UBE2J2	XP_417582.1
Danio rerio (zebrafish)	ube2j2	NP_001083004.1
Drosophila melanogaster (fruit fly)	CG5823	NP_650631.1
Caenorhabditis elegans (roundworm)	ubc-26	NP_871922.1
Saccharomyces cerevisiae (baker's yeast)	UBC6	NP_011026.3
Eremothecium gossypii	AGOS_AGR372W	NP_987038.1
Arabidopsis thaliana (thale cress)	UBC34	NP_001077554.1
Pan troglodytes (chimpanzee)	UBE2J2	XP_513708.4
Canis lupus familiaris (dog)	UBE2J2	XP_848334.1
Mus musculus (house mouse)	Ube2j2	NP_001034246.1
Rattus norvegicus (Norway rat)	Ube2j2	NP_001007656.1
Schizosaccharomyces pombe (fission yeast)	ubc6	NP_593256.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005954	XP_315994.4
Xenopus tropicalis (tropical clawed frog)	ube2j2	NP_001106369.1

Gene Ontology
Bibliography

Related articles in PubMed

A high-coverage shRNA screen identifies TMEM129 as an E3 ligase involved in ER-associated protein degradation.
van de Weijer ML, Bassik MC, Luteijn RD, Voorburg CM, Lohuis MA, Kremmer E, Hoeben RC, LeProust EM, Chen S, Hoelen H, Ressing ME, Patena W, Weissman JS, McManus MT, Wiertz EJ, Lebbink RJ
Nature communications53832(2014 May)

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
El Khouri E, Le Pavec G, Toledano MB, Delaunay-Moisan A
The Journal of biological chemistry288(43)31177-91(2013 Oct)

The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
David Y, Ziv T, Admon A, Navon A
The Journal of biological chemistry285(12)8595-604(2010 Mar)

Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s).
Tiwari S, Weissman AM
The Journal of biological chemistry276(19)16193-200(2001 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ube2j2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2j2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa28997	NM_001007655.2 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa11393	XM_006239558.3 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa11393	XM_008764355.2 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa11393	XM_006239557.3 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa28997	XM_006239554.3 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa28997	XM_006239555.3 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa28997	XM_008764354.2 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2j2 ( NM_001007655.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001007656.2 Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), mRNA.

Price & Availability↑

CloneID	ORa28997
Clone ID Related Accession (Same CDS sequence)	XM_008764354.2 , XM_006239554.3 , XM_006239555.3 , NM_001007655.2
Accession Version	NM_001007655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 J2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126156.5 and BC079115.1. On or before Jun 23, 2015 this sequence version replaced XM_008764352.1, NM_001007655.1. ##Evidence-Data-START## Transcript exon combination :: BC079115.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATCAGG CAAAAGCCCT TGATTCTGCC AGAAAGATGA GCAGTAACAG CAATAAGAGA 
GCTCCAACAA CTGCGACACA AAGGTTGAAA CAGGACTACC TTCGAATTAA GAAAGACCCA 
GTGCCTTACA TCTGTGCGGA GCCCCTCCCT TCAAATATTC TTGAATGGCA TTATGTTGTC 
CGAGGCCCTG AGATGACTCC TTATGAAGGT GGCTATTATC ATGGAAAACT AATTTTTCCC 
AGAGAATTTC CATTTAAGCC TCCTAGTATT TACATGATAA CCCCCAATGG AAGATTTAAG 
TGCAACACAA GGCTGTGTCT CTCCATCACA GATTTCCATC CAGATACATG GAATCCAGCG 
TGGTCCGTCT CCACCATTCT AACAGGGCTC CTGAGCTTTA TGGTGGAGAA AGGCCCCACC 
CTGGGGAGCA TAGAGACATC GGACTTCACG AAAAAACAGC TGGCAGCACA GAGTCTAGTG 
TTTAATTTAA AAGATAAAGT CTTCTGTGAA TTATTTCCTG AAGTTGTGGA GGAAATTAAA 
CAGAAACAGA AAGCACAAGA TGAACTAAGT AACAGGCCCC AGAATCTTCC TTTGCCAGAC 
GTGGTTCCTG ATGGGGAGAT GCACCGTGGC CAGAATGGGA TCCAGCTCCT CAATGGGCAT 
GCACCTGCTG CTGGCCCAAA CCTTGCTGGG CTCCCACAGG CCAACAGGCA TCATGGACTC 
CTGGGCGGCG CTTTGGCGAA CTTGTTTGTT ATAGTTGGGT TTGCGGCCTT TGCCTACACG 
GTCAAATACG TGCTGAGAAG CATAGCACAG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007656.2
CDS	463..1278
Translation	MHQAKALDSARKMSSNSNKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIKQKQKAQDELSNRPQNLPLPDVVPDGEMHRGQNGIQLLNGHAPAAGPNLAGLPQANRHHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE

Target ORF information:

RefSeq Version	NM_001007655.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007655.2

ORF Insert Sequence:

ATGCATCAGG CAAAAGCCCT TGATTCTGCC AGAAAGATGA GCAGTAACAG CAATAAGAGA 
GCTCCAACAA CTGCGACACA AAGGTTGAAA CAGGACTACC TTCGAATTAA GAAAGACCCA 
GTGCCTTACA TCTGTGCGGA GCCCCTCCCT TCAAATATTC TTGAATGGCA TTATGTTGTC 
CGAGGCCCTG AGATGACTCC TTATGAAGGT GGCTATTATC ATGGAAAACT AATTTTTCCC 
AGAGAATTTC CATTTAAGCC TCCTAGTATT TACATGATAA CCCCCAATGG AAGATTTAAG 
TGCAACACAA GGCTGTGTCT CTCCATCACA GATTTCCATC CAGATACATG GAATCCAGCG 
TGGTCCGTCT CCACCATTCT AACAGGGCTC CTGAGCTTTA TGGTGGAGAA AGGCCCCACC 
CTGGGGAGCA TAGAGACATC GGACTTCACG AAAAAACAGC TGGCAGCACA GAGTCTAGTG 
TTTAATTTAA AAGATAAAGT CTTCTGTGAA TTATTTCCTG AAGTTGTGGA GGAAATTAAA 
CAGAAACAGA AAGCACAAGA TGAACTAAGT AACAGGCCCC AGAATCTTCC TTTGCCAGAC 
GTGGTTCCTG ATGGGGAGAT GCACCGTGGC CAGAATGGGA TCCAGCTCCT CAATGGGCAT 
GCACCTGCTG CTGGCCCAAA CCTTGCTGGG CTCCCACAGG CCAACAGGCA TCATGGACTC 
CTGGGCGGCG CTTTGGCGAA CTTGTTTGTT ATAGTTGGGT TTGCGGCCTT TGCCTACACG 
GTCAAATACG TGCTGAGAAG CATAGCACAG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2j2 ( XM_008764355.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762577.1 Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa11393
Clone ID Related Accession (Same CDS sequence)	XM_008764355.2 , XM_006239558.3 , XM_006239557.3
Accession Version	XM_008764355.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 J2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGTA ACAGCAATAA GAGAGCTCCA ACAACTGCGA CACAAAGGTT GAAACAGGAC 
TACCTTCGAA TTAAGAAAGA CCCAGTGCCT TACATCTGTG CGGAGCCCCT CCCTTCAAAT 
ATTCTTGAAT GGCATTATGT TGTCCGAGGC CCTGAGATGA CTCCTTATGA AGGTGGCTAT 
TATCATGGAA AACTAATTTT TCCCAGAGAA TTTCCATTTA AGCCTCCTAG TATTTACATG 
ATAACCCCCA ATGGAAGATT TAAGTGCAAC ACAAGGCTGT GTCTCTCCAT CACAGATTTC 
CATCCAGATA CATGGAATCC AGCGTGGTCC GTCTCCACCA TTCTAACAGG GCTCCTGAGC 
TTTATGGTGG AGAAAGGCCC CACCCTGGGG AGCATAGAGA CATCGGACTT CACGAAAAAA 
CAGCTGGCAG CACAGAGTCT AGTGTTTAAT TTAAAAGATA AAGTCTTCTG TGAATTATTT 
CCTGAAGTTG TGGAGGAAAT TAAACAGAAA CAGAAAGCAC AAGATGAACT AAGTAACAGG 
CCCCAGAATC TTCCTTTGCC AGACGTGGTT CCTGATGGGG AGATGCACCG TGGCCAGAAT 
GGGATCCAGC TCCTCAATGG GCATGCACCT GCTGCTGGCC CAAACCTTGC TGGGCTCCCA 
CAGGCCAACA GGCATCATGG ACTCCTGGGC GGCGCTTTGG CGAACTTGTT TGTTATAGTT 
GGGTTTGCGG CCTTTGCCTA CACGGTCAAA TACGTGCTGA GAAGCATAGC ACAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762577.1
CDS	1622..2401
Translation	MSSNSNKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIKQKQKAQDELSNRPQNLPLPDVVPDGEMHRGQNGIQLLNGHAPAAGPNLAGLPQANRHHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE

Target ORF information:

RefSeq Version	XM_008764355.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764355.2

ORF Insert Sequence:

ATGAGCAGTA ACAGCAATAA GAGAGCTCCA ACAACTGCGA CACAAAGGTT GAAACAGGAC 
TACCTTCGAA TTAAGAAAGA CCCAGTGCCT TACATCTGTG CGGAGCCCCT CCCTTCAAAT 
ATTCTTGAAT GGCATTATGT TGTCCGAGGC CCTGAGATGA CTCCTTATGA AGGTGGCTAT 
TATCATGGAA AACTAATTTT TCCCAGAGAA TTTCCATTTA AGCCTCCTAG TATTTACATG 
ATAACCCCCA ATGGAAGATT TAAGTGCAAC ACAAGGCTGT GTCTCTCCAT CACAGATTTC 
CATCCAGATA CATGGAATCC AGCGTGGTCC GTCTCCACCA TTCTAACAGG GCTCCTGAGC 
TTTATGGTGG AGAAAGGCCC CACCCTGGGG AGCATAGAGA CATCGGACTT CACGAAAAAA 
CAGCTGGCAG CACAGAGTCT AGTGTTTAAT TTAAAAGATA AAGTCTTCTG TGAATTATTT 
CCTGAAGTTG TGGAGGAAAT TAAACAGAAA CAGAAAGCAC AAGATGAACT AAGTAACAGG 
CCCCAGAATC TTCCTTTGCC AGACGTGGTT CCTGATGGGG AGATGCACCG TGGCCAGAAT 
GGGATCCAGC TCCTCAATGG GCATGCACCT GCTGCTGGCC CAAACCTTGC TGGGCTCCCA 
CAGGCCAACA GGCATCATGG ACTCCTGGGC GGCGCTTTGG CGAACTTGTT TGTTATAGTT 
GGGTTTGCGG CCTTTGCCTA CACGGTCAAA TACGTGCTGA GAAGCATAGC ACAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2j2 ( XM_006239558.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239620.1 Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa11393
Clone ID Related Accession (Same CDS sequence)	XM_008764355.2 , XM_006239558.3 , XM_006239557.3
Accession Version	XM_006239558.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 J2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239558.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGTA ACAGCAATAA GAGAGCTCCA ACAACTGCGA CACAAAGGTT GAAACAGGAC 
TACCTTCGAA TTAAGAAAGA CCCAGTGCCT TACATCTGTG CGGAGCCCCT CCCTTCAAAT 
ATTCTTGAAT GGCATTATGT TGTCCGAGGC CCTGAGATGA CTCCTTATGA AGGTGGCTAT 
TATCATGGAA AACTAATTTT TCCCAGAGAA TTTCCATTTA AGCCTCCTAG TATTTACATG 
ATAACCCCCA ATGGAAGATT TAAGTGCAAC ACAAGGCTGT GTCTCTCCAT CACAGATTTC 
CATCCAGATA CATGGAATCC AGCGTGGTCC GTCTCCACCA TTCTAACAGG GCTCCTGAGC 
TTTATGGTGG AGAAAGGCCC CACCCTGGGG AGCATAGAGA CATCGGACTT CACGAAAAAA 
CAGCTGGCAG CACAGAGTCT AGTGTTTAAT TTAAAAGATA AAGTCTTCTG TGAATTATTT 
CCTGAAGTTG TGGAGGAAAT TAAACAGAAA CAGAAAGCAC AAGATGAACT AAGTAACAGG 
CCCCAGAATC TTCCTTTGCC AGACGTGGTT CCTGATGGGG AGATGCACCG TGGCCAGAAT 
GGGATCCAGC TCCTCAATGG GCATGCACCT GCTGCTGGCC CAAACCTTGC TGGGCTCCCA 
CAGGCCAACA GGCATCATGG ACTCCTGGGC GGCGCTTTGG CGAACTTGTT TGTTATAGTT 
GGGTTTGCGG CCTTTGCCTA CACGGTCAAA TACGTGCTGA GAAGCATAGC ACAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239620.1
CDS	115..894
Translation	MSSNSNKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIKQKQKAQDELSNRPQNLPLPDVVPDGEMHRGQNGIQLLNGHAPAAGPNLAGLPQANRHHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE

Target ORF information:

RefSeq Version	XM_006239558.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239558.3

ORF Insert Sequence:

ATGAGCAGTA ACAGCAATAA GAGAGCTCCA ACAACTGCGA CACAAAGGTT GAAACAGGAC 
TACCTTCGAA TTAAGAAAGA CCCAGTGCCT TACATCTGTG CGGAGCCCCT CCCTTCAAAT 
ATTCTTGAAT GGCATTATGT TGTCCGAGGC CCTGAGATGA CTCCTTATGA AGGTGGCTAT 
TATCATGGAA AACTAATTTT TCCCAGAGAA TTTCCATTTA AGCCTCCTAG TATTTACATG 
ATAACCCCCA ATGGAAGATT TAAGTGCAAC ACAAGGCTGT GTCTCTCCAT CACAGATTTC 
CATCCAGATA CATGGAATCC AGCGTGGTCC GTCTCCACCA TTCTAACAGG GCTCCTGAGC 
TTTATGGTGG AGAAAGGCCC CACCCTGGGG AGCATAGAGA CATCGGACTT CACGAAAAAA 
CAGCTGGCAG CACAGAGTCT AGTGTTTAAT TTAAAAGATA AAGTCTTCTG TGAATTATTT 
CCTGAAGTTG TGGAGGAAAT TAAACAGAAA CAGAAAGCAC AAGATGAACT AAGTAACAGG 
CCCCAGAATC TTCCTTTGCC AGACGTGGTT CCTGATGGGG AGATGCACCG TGGCCAGAAT 
GGGATCCAGC TCCTCAATGG GCATGCACCT GCTGCTGGCC CAAACCTTGC TGGGCTCCCA 
CAGGCCAACA GGCATCATGG ACTCCTGGGC GGCGCTTTGG CGAACTTGTT TGTTATAGTT 
GGGTTTGCGG CCTTTGCCTA CACGGTCAAA TACGTGCTGA GAAGCATAGC ACAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2j2 ( XM_006239557.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239619.1 Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa11393
Clone ID Related Accession (Same CDS sequence)	XM_008764355.2 , XM_006239558.3 , XM_006239557.3
Accession Version	XM_006239557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 J2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239557.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGTA ACAGCAATAA GAGAGCTCCA ACAACTGCGA CACAAAGGTT GAAACAGGAC 
TACCTTCGAA TTAAGAAAGA CCCAGTGCCT TACATCTGTG CGGAGCCCCT CCCTTCAAAT 
ATTCTTGAAT GGCATTATGT TGTCCGAGGC CCTGAGATGA CTCCTTATGA AGGTGGCTAT 
TATCATGGAA AACTAATTTT TCCCAGAGAA TTTCCATTTA AGCCTCCTAG TATTTACATG 
ATAACCCCCA ATGGAAGATT TAAGTGCAAC ACAAGGCTGT GTCTCTCCAT CACAGATTTC 
CATCCAGATA CATGGAATCC AGCGTGGTCC GTCTCCACCA TTCTAACAGG GCTCCTGAGC 
TTTATGGTGG AGAAAGGCCC CACCCTGGGG AGCATAGAGA CATCGGACTT CACGAAAAAA 
CAGCTGGCAG CACAGAGTCT AGTGTTTAAT TTAAAAGATA AAGTCTTCTG TGAATTATTT 
CCTGAAGTTG TGGAGGAAAT TAAACAGAAA CAGAAAGCAC AAGATGAACT AAGTAACAGG 
CCCCAGAATC TTCCTTTGCC AGACGTGGTT CCTGATGGGG AGATGCACCG TGGCCAGAAT 
GGGATCCAGC TCCTCAATGG GCATGCACCT GCTGCTGGCC CAAACCTTGC TGGGCTCCCA 
CAGGCCAACA GGCATCATGG ACTCCTGGGC GGCGCTTTGG CGAACTTGTT TGTTATAGTT 
GGGTTTGCGG CCTTTGCCTA CACGGTCAAA TACGTGCTGA GAAGCATAGC ACAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239619.1
CDS	286..1065
Translation	MSSNSNKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIKQKQKAQDELSNRPQNLPLPDVVPDGEMHRGQNGIQLLNGHAPAAGPNLAGLPQANRHHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE

Target ORF information:

RefSeq Version	XM_006239557.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239557.3

ORF Insert Sequence:

ATGAGCAGTA ACAGCAATAA GAGAGCTCCA ACAACTGCGA CACAAAGGTT GAAACAGGAC 
TACCTTCGAA TTAAGAAAGA CCCAGTGCCT TACATCTGTG CGGAGCCCCT CCCTTCAAAT 
ATTCTTGAAT GGCATTATGT TGTCCGAGGC CCTGAGATGA CTCCTTATGA AGGTGGCTAT 
TATCATGGAA AACTAATTTT TCCCAGAGAA TTTCCATTTA AGCCTCCTAG TATTTACATG 
ATAACCCCCA ATGGAAGATT TAAGTGCAAC ACAAGGCTGT GTCTCTCCAT CACAGATTTC 
CATCCAGATA CATGGAATCC AGCGTGGTCC GTCTCCACCA TTCTAACAGG GCTCCTGAGC 
TTTATGGTGG AGAAAGGCCC CACCCTGGGG AGCATAGAGA CATCGGACTT CACGAAAAAA 
CAGCTGGCAG CACAGAGTCT AGTGTTTAAT TTAAAAGATA AAGTCTTCTG TGAATTATTT 
CCTGAAGTTG TGGAGGAAAT TAAACAGAAA CAGAAAGCAC AAGATGAACT AAGTAACAGG 
CCCCAGAATC TTCCTTTGCC AGACGTGGTT CCTGATGGGG AGATGCACCG TGGCCAGAAT 
GGGATCCAGC TCCTCAATGG GCATGCACCT GCTGCTGGCC CAAACCTTGC TGGGCTCCCA 
CAGGCCAACA GGCATCATGG ACTCCTGGGC GGCGCTTTGG CGAACTTGTT TGTTATAGTT 
GGGTTTGCGG CCTTTGCCTA CACGGTCAAA TACGTGCTGA GAAGCATAGC ACAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2j2 ( XM_008764354.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762576.1 Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa28997
Clone ID Related Accession (Same CDS sequence)	XM_008764354.2 , XM_006239554.3 , XM_006239555.3 , NM_001007655.2
Accession Version	XM_008764354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 J2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCAGG CAAAAGCCCT TGATTCTGCC AGAAAGATGA GCAGTAACAG CAATAAGAGA 
GCTCCAACAA CTGCGACACA AAGGTTGAAA CAGGACTACC TTCGAATTAA GAAAGACCCA 
GTGCCTTACA TCTGTGCGGA GCCCCTCCCT TCAAATATTC TTGAATGGCA TTATGTTGTC 
CGAGGCCCTG AGATGACTCC TTATGAAGGT GGCTATTATC ATGGAAAACT AATTTTTCCC 
AGAGAATTTC CATTTAAGCC TCCTAGTATT TACATGATAA CCCCCAATGG AAGATTTAAG 
TGCAACACAA GGCTGTGTCT CTCCATCACA GATTTCCATC CAGATACATG GAATCCAGCG 
TGGTCCGTCT CCACCATTCT AACAGGGCTC CTGAGCTTTA TGGTGGAGAA AGGCCCCACC 
CTGGGGAGCA TAGAGACATC GGACTTCACG AAAAAACAGC TGGCAGCACA GAGTCTAGTG 
TTTAATTTAA AAGATAAAGT CTTCTGTGAA TTATTTCCTG AAGTTGTGGA GGAAATTAAA 
CAGAAACAGA AAGCACAAGA TGAACTAAGT AACAGGCCCC AGAATCTTCC TTTGCCAGAC 
GTGGTTCCTG ATGGGGAGAT GCACCGTGGC CAGAATGGGA TCCAGCTCCT CAATGGGCAT 
GCACCTGCTG CTGGCCCAAA CCTTGCTGGG CTCCCACAGG CCAACAGGCA TCATGGACTC 
CTGGGCGGCG CTTTGGCGAA CTTGTTTGTT ATAGTTGGGT TTGCGGCCTT TGCCTACACG 
GTCAAATACG TGCTGAGAAG CATAGCACAG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762576.1
CDS	582..1397
Translation	MHQAKALDSARKMSSNSNKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIKQKQKAQDELSNRPQNLPLPDVVPDGEMHRGQNGIQLLNGHAPAAGPNLAGLPQANRHHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE

Target ORF information:

RefSeq Version	XM_008764354.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764354.2

ORF Insert Sequence:

ATGCATCAGG CAAAAGCCCT TGATTCTGCC AGAAAGATGA GCAGTAACAG CAATAAGAGA 
GCTCCAACAA CTGCGACACA AAGGTTGAAA CAGGACTACC TTCGAATTAA GAAAGACCCA 
GTGCCTTACA TCTGTGCGGA GCCCCTCCCT TCAAATATTC TTGAATGGCA TTATGTTGTC 
CGAGGCCCTG AGATGACTCC TTATGAAGGT GGCTATTATC ATGGAAAACT AATTTTTCCC 
AGAGAATTTC CATTTAAGCC TCCTAGTATT TACATGATAA CCCCCAATGG AAGATTTAAG 
TGCAACACAA GGCTGTGTCT CTCCATCACA GATTTCCATC CAGATACATG GAATCCAGCG 
TGGTCCGTCT CCACCATTCT AACAGGGCTC CTGAGCTTTA TGGTGGAGAA AGGCCCCACC 
CTGGGGAGCA TAGAGACATC GGACTTCACG AAAAAACAGC TGGCAGCACA GAGTCTAGTG 
TTTAATTTAA AAGATAAAGT CTTCTGTGAA TTATTTCCTG AAGTTGTGGA GGAAATTAAA 
CAGAAACAGA AAGCACAAGA TGAACTAAGT AACAGGCCCC AGAATCTTCC TTTGCCAGAC 
GTGGTTCCTG ATGGGGAGAT GCACCGTGGC CAGAATGGGA TCCAGCTCCT CAATGGGCAT 
GCACCTGCTG CTGGCCCAAA CCTTGCTGGG CTCCCACAGG CCAACAGGCA TCATGGACTC 
CTGGGCGGCG CTTTGGCGAA CTTGTTTGTT ATAGTTGGGT TTGCGGCCTT TGCCTACACG 
GTCAAATACG TGCTGAGAAG CATAGCACAG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2j2 ( XM_006239554.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239616.1 Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa28997
Clone ID Related Accession (Same CDS sequence)	XM_008764354.2 , XM_006239554.3 , XM_006239555.3 , NM_001007655.2
Accession Version	XM_006239554.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 J2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239554.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCAGG CAAAAGCCCT TGATTCTGCC AGAAAGATGA GCAGTAACAG CAATAAGAGA 
GCTCCAACAA CTGCGACACA AAGGTTGAAA CAGGACTACC TTCGAATTAA GAAAGACCCA 
GTGCCTTACA TCTGTGCGGA GCCCCTCCCT TCAAATATTC TTGAATGGCA TTATGTTGTC 
CGAGGCCCTG AGATGACTCC TTATGAAGGT GGCTATTATC ATGGAAAACT AATTTTTCCC 
AGAGAATTTC CATTTAAGCC TCCTAGTATT TACATGATAA CCCCCAATGG AAGATTTAAG 
TGCAACACAA GGCTGTGTCT CTCCATCACA GATTTCCATC CAGATACATG GAATCCAGCG 
TGGTCCGTCT CCACCATTCT AACAGGGCTC CTGAGCTTTA TGGTGGAGAA AGGCCCCACC 
CTGGGGAGCA TAGAGACATC GGACTTCACG AAAAAACAGC TGGCAGCACA GAGTCTAGTG 
TTTAATTTAA AAGATAAAGT CTTCTGTGAA TTATTTCCTG AAGTTGTGGA GGAAATTAAA 
CAGAAACAGA AAGCACAAGA TGAACTAAGT AACAGGCCCC AGAATCTTCC TTTGCCAGAC 
GTGGTTCCTG ATGGGGAGAT GCACCGTGGC CAGAATGGGA TCCAGCTCCT CAATGGGCAT 
GCACCTGCTG CTGGCCCAAA CCTTGCTGGG CTCCCACAGG CCAACAGGCA TCATGGACTC 
CTGGGCGGCG CTTTGGCGAA CTTGTTTGTT ATAGTTGGGT TTGCGGCCTT TGCCTACACG 
GTCAAATACG TGCTGAGAAG CATAGCACAG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239616.1
CDS	278..1093
Translation	MHQAKALDSARKMSSNSNKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIKQKQKAQDELSNRPQNLPLPDVVPDGEMHRGQNGIQLLNGHAPAAGPNLAGLPQANRHHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE

Target ORF information:

RefSeq Version	XM_006239554.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239554.3

ORF Insert Sequence:

ATGCATCAGG CAAAAGCCCT TGATTCTGCC AGAAAGATGA GCAGTAACAG CAATAAGAGA 
GCTCCAACAA CTGCGACACA AAGGTTGAAA CAGGACTACC TTCGAATTAA GAAAGACCCA 
GTGCCTTACA TCTGTGCGGA GCCCCTCCCT TCAAATATTC TTGAATGGCA TTATGTTGTC 
CGAGGCCCTG AGATGACTCC TTATGAAGGT GGCTATTATC ATGGAAAACT AATTTTTCCC 
AGAGAATTTC CATTTAAGCC TCCTAGTATT TACATGATAA CCCCCAATGG AAGATTTAAG 
TGCAACACAA GGCTGTGTCT CTCCATCACA GATTTCCATC CAGATACATG GAATCCAGCG 
TGGTCCGTCT CCACCATTCT AACAGGGCTC CTGAGCTTTA TGGTGGAGAA AGGCCCCACC 
CTGGGGAGCA TAGAGACATC GGACTTCACG AAAAAACAGC TGGCAGCACA GAGTCTAGTG 
TTTAATTTAA AAGATAAAGT CTTCTGTGAA TTATTTCCTG AAGTTGTGGA GGAAATTAAA 
CAGAAACAGA AAGCACAAGA TGAACTAAGT AACAGGCCCC AGAATCTTCC TTTGCCAGAC 
GTGGTTCCTG ATGGGGAGAT GCACCGTGGC CAGAATGGGA TCCAGCTCCT CAATGGGCAT 
GCACCTGCTG CTGGCCCAAA CCTTGCTGGG CTCCCACAGG CCAACAGGCA TCATGGACTC 
CTGGGCGGCG CTTTGGCGAA CTTGTTTGTT ATAGTTGGGT TTGCGGCCTT TGCCTACACG 
GTCAAATACG TGCTGAGAAG CATAGCACAG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2j2 ( XM_006239555.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239617.1 Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa28997
Clone ID Related Accession (Same CDS sequence)	XM_008764354.2 , XM_006239554.3 , XM_006239555.3 , NM_001007655.2
Accession Version	XM_006239555.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 J2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239555.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCAGG CAAAAGCCCT TGATTCTGCC AGAAAGATGA GCAGTAACAG CAATAAGAGA 
GCTCCAACAA CTGCGACACA AAGGTTGAAA CAGGACTACC TTCGAATTAA GAAAGACCCA 
GTGCCTTACA TCTGTGCGGA GCCCCTCCCT TCAAATATTC TTGAATGGCA TTATGTTGTC 
CGAGGCCCTG AGATGACTCC TTATGAAGGT GGCTATTATC ATGGAAAACT AATTTTTCCC 
AGAGAATTTC CATTTAAGCC TCCTAGTATT TACATGATAA CCCCCAATGG AAGATTTAAG 
TGCAACACAA GGCTGTGTCT CTCCATCACA GATTTCCATC CAGATACATG GAATCCAGCG 
TGGTCCGTCT CCACCATTCT AACAGGGCTC CTGAGCTTTA TGGTGGAGAA AGGCCCCACC 
CTGGGGAGCA TAGAGACATC GGACTTCACG AAAAAACAGC TGGCAGCACA GAGTCTAGTG 
TTTAATTTAA AAGATAAAGT CTTCTGTGAA TTATTTCCTG AAGTTGTGGA GGAAATTAAA 
CAGAAACAGA AAGCACAAGA TGAACTAAGT AACAGGCCCC AGAATCTTCC TTTGCCAGAC 
GTGGTTCCTG ATGGGGAGAT GCACCGTGGC CAGAATGGGA TCCAGCTCCT CAATGGGCAT 
GCACCTGCTG CTGGCCCAAA CCTTGCTGGG CTCCCACAGG CCAACAGGCA TCATGGACTC 
CTGGGCGGCG CTTTGGCGAA CTTGTTTGTT ATAGTTGGGT TTGCGGCCTT TGCCTACACG 
GTCAAATACG TGCTGAGAAG CATAGCACAG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239617.1
CDS	594..1409
Translation	MHQAKALDSARKMSSNSNKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIKQKQKAQDELSNRPQNLPLPDVVPDGEMHRGQNGIQLLNGHAPAAGPNLAGLPQANRHHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE

Target ORF information:

RefSeq Version	XM_006239555.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2, J2 (Ube2j2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239555.3

ORF Insert Sequence:

ATGCATCAGG CAAAAGCCCT TGATTCTGCC AGAAAGATGA GCAGTAACAG CAATAAGAGA 
GCTCCAACAA CTGCGACACA AAGGTTGAAA CAGGACTACC TTCGAATTAA GAAAGACCCA 
GTGCCTTACA TCTGTGCGGA GCCCCTCCCT TCAAATATTC TTGAATGGCA TTATGTTGTC 
CGAGGCCCTG AGATGACTCC TTATGAAGGT GGCTATTATC ATGGAAAACT AATTTTTCCC 
AGAGAATTTC CATTTAAGCC TCCTAGTATT TACATGATAA CCCCCAATGG AAGATTTAAG 
TGCAACACAA GGCTGTGTCT CTCCATCACA GATTTCCATC CAGATACATG GAATCCAGCG 
TGGTCCGTCT CCACCATTCT AACAGGGCTC CTGAGCTTTA TGGTGGAGAA AGGCCCCACC 
CTGGGGAGCA TAGAGACATC GGACTTCACG AAAAAACAGC TGGCAGCACA GAGTCTAGTG 
TTTAATTTAA AAGATAAAGT CTTCTGTGAA TTATTTCCTG AAGTTGTGGA GGAAATTAAA 
CAGAAACAGA AAGCACAAGA TGAACTAAGT AACAGGCCCC AGAATCTTCC TTTGCCAGAC 
GTGGTTCCTG ATGGGGAGAT GCACCGTGGC CAGAATGGGA TCCAGCTCCT CAATGGGCAT 
GCACCTGCTG CTGGCCCAAA CCTTGCTGGG CTCCCACAGG CCAACAGGCA TCATGGACTC 
CTGGGCGGCG CTTTGGCGAA CTTGTTTGTT ATAGTTGGGT TTGCGGCCTT TGCCTACACG 
GTCAAATACG TGCTGAGAAG CATAGCACAG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A high-coverage shRNA screen identifies TMEM129 as an E3 ligase involved in ER-associated protein degradation.
Nature communications53832(2014 May)
van de Weijer ML,Bassik MC,Luteijn RD,Voorburg CM,Lohuis MA,Kremmer E,Hoeben RC,LeProust EM,Chen S,Hoelen H,Ressing ME,Patena W,Weissman JS,McManus MT,Wiertz EJ,Lebbink RJ

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
The Journal of biological chemistry288(43)31177-91(2013 Oct)
El Khouri E,Le Pavec G,Toledano MB,Delaunay-Moisan A

The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
The Journal of biological chemistry285(12)8595-604(2010 Mar)
David Y,Ziv T,Admon A,Navon A

Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s).
The Journal of biological chemistry276(19)16193-200(2001 May)
Tiwari S,Weissman AM

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Ube2j2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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