Gba2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gba2
Entrez Gene ID	298399
Full Name	glucosylceramidase beta 2
General protein information	Preferred Names glucosylceramidase beta 2 Names non-lysosomal glucosylceramidase NLGase beta-glucocerebrosidase 2 beta-glucosidase 2 bile acid beta-glucosidase glucosidase beta 2 glucosylceramidase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013091.2	NP_001013109.2	non-lysosomal glucosylceramidase

Pathways from BioSystems

Homologies

	GBA2	XP_001084705.1
Homo sapiens (human)	GBA2	NP_065995.1
Mus musculus (house mouse)	Gba2	NP_766280.2
Gallus gallus (chicken)	GBA2	XP_003642968.1
Arabidopsis thaliana (thale cress)	AT3G24180	NP_189060.2
Bos taurus (cattle)	GBA2	XP_002689726.1
Danio rerio (zebrafish)	gba2	XP_687652.2
Drosophila melanogaster (fruit fly)	CG33090	NP_788055.2
Caenorhabditis elegans (roundworm)	R08F11.1	NP_504047.2
Pan troglodytes (chimpanzee)	GBA2	XP_001167923.2
Canis lupus familiaris (dog)	GBA2	XP_005626843.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008830	XP_319575.4
Oryza sativa (rice)	Os10g0473400	NP_001064833.1
Xenopus tropicalis (tropical clawed frog)	gba2	XP_002943495.2
Rattus norvegicus (Norway rat)	Gba2	NP_001013109.2
Caenorhabditis elegans (roundworm)	hpo-13	NP_001021680.1

Gene Ontology
Bibliography

Related articles in PubMed

Glucosylated cholesterol in mammalian cells and tissues: formation and degradation by multiple cellular ?-glucosidases.
Marques AR, Mirzaian M, Akiyama H, Wisse P, Ferraz MJ, Gaspar P, Ghauharali-van der Vlugt K, Meijer R, Giraldo P, Alfonso P, Ir?n P, Dahl M, Karlsson S, Pavlova EV, Cox TM, Scheij S, Verhoek M, Ottenhoff R, van Roomen CP, Pannu NS, van Eijk M, Dekker N, Boot RG, Overkleeft HS, Blommaart E, Hirabayashi Y, Aerts JM
Journal of lipid research57(3)451-63(2016 Mar)

Accumulation of glucosylceramide in the absence of the beta-glucosidase GBA2 alters cytoskeletal dynamics.
Raju D, Schonauer S, Hamzeh H, Flynn KC, Bradke F, Vom Dorp K, D?rmann P, Yildiz Y, Tr?tschel C, Poetsch A, Breiden B, Sandhoff K, K?rschen HG, Wachten D
PLoS genetics11(3)e1005063(2015 Mar)

The non-lysosomal ?-glucosidase GBA2 is a non-integral membrane-associated protein at the endoplasmic reticulum (ER) and Golgi.
K?rschen HG, Yildiz Y, Raju DN, Schonauer S, B?nigk W, Jansen V, Kremmer E, Kaupp UB, Wachten D
The Journal of biological chemistry288(5)3381-93(2013 Feb)

Loss of function of glucocerebrosidase GBA2 is responsible for motor neuron defects in hereditary spastic paraplegia.
Martin E, Sch?le R, Smets K, Rastetter A, Boukhris A, Loureiro JL, Gonzalez MA, Mundwiller E, Deconinck T, Wessner M, Jornea L, Oteyza AC, Durr A, Martin JJ, Sch?ls L, Mhiri C, Lamari F, Z?chner S, De Jonghe P, Kabashi E, Brice A, Stevanin G
American journal of human genetics92(2)238-44(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gba2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gba2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03333	NM_001013091.2 Latest version!	Rattus norvegicus glucosylceramidase beta 2 (Gba2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gba2 ( NM_001013091.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001013109.2 Rattus norvegicus glucosylceramidase beta 2 (Gba2), mRNA.

Price & Availability↑

CloneID	ORa03333
Clone ID Related Accession (Same CDS sequence)	NM_001013091.2
Accession Version	NM_001013091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-20
Organism	Rattus norvegicus(Norway rat)
Product	non-lysosomal glucosylceramidase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121204.5. On or before Jun 1, 2008 this sequence version replaced XM_216490.4, XM_001069986.1, NM_001013091.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGTAACCT GCGTACCTGC CTCCGAGCAG ATCGGCTGTG CGGAAAGAGA TTCACAAATT 
TATAGTGAAG ATACTGGAGG CACTGAGGCT GTGCGGGTTA CAGACTGCCG GAGCCCGGAG 
GACAGTGGAC CCCAGAATGA GCCAGGCTAC TGCAATTCAG AGGACTCTGG GCAGCTGATG 
GCCTCCTACG AGGGTAAAGC TAGGGGCTAC CAGGTGCCTC CTTTTGGCTG GCGGATTTGC 
TTGGCTCACG AGTTTGCAGA GAAGAGGAAA CCTTTTCAGG CTAACAACGT CTCTCTCAGT 
AATTTGGTAA AGCATTTCGG TATGGGCTTG AGGTACTTGA AGTGGTGGTA CCGGAAGACG 
CAGGTGGAAA AGAAGACCCC TTTCATCGAC ATGTTCAATT CTGTACCCCT GAGACAGATC 
TACGGTTGCC CCTTGGGTGG CATTGGAGGA GGCACCATCA CCCGGGGCTG GAGAGGCCAG 
TTCTGTCGTT GGCAGCTTAA TCCTGGAATG TACCAGCACC AGACAGTCAT TGCAGACCAA 
TTTATAGTAT GCTTGCGTCG AGATGGGCGG ACTGTGTATC AGCAAGTCCT GTCCTTGGAG 
CTTCCGAGTG TCCTGCGCAG CTGGAACTGG GGCCTGTGTG GTTATTTTGC TTTCTACCAC 
GCCCTCTATC CCCGAGCCTG GACAGTCTAT CAGCTTCCTG GCCAGAATGT CACCCTCACC 
TGTCGCCAGA TCACACCTAT CTTGCCTCAT GATTACCAGG ACAGCAGCCT CCCCGTAGGA 
GTGTTTGTGT GGGATGTAGA GAATGAAGGA GATGAGACTC TGGATGTGTC CATCATGTTC 
TCCATGCGGA ATGGACTAGG AGGTGAAGAT GATGCTGCCG GAGGTTTGTG GAACGAGCCC 
TTCCGTCTGG AACAGGATGG GACGACTGTA CAGGGGCTAC TCCTGCATCA TCCAACCCCT 
CCGAACCCCT ACACCATGGC TGTGGCTGCG CGACACACGG CAGATACCAC AGTAACCTAC 
ACCACAGCCT TTGACCCCGA CAGCACTGGG CAGCAGGTGT GGCAGGACCT ACTTCAAGAT 
GGACAGCTGG ACTCCCCTGC TGGCCAGAGC ACCCCGACAC AGAGAGGAGA GGGTGTCGCT 
GGGGCTGTAT GTGCCTCCAG CAAGCTGCTG CCACGAGGCC GGTGCTGCTT GGAGTTCTCA 
CTGGCTTGGG ATATGCCTAG GATCATGTTT GGAGCCAAAG GCCAAGTCCA CTACAGGCGG 
TATACACGGT TCTTTGGTTC AGATGGTGAT GTGGCGCCTG CCCTGAGCCA CTATGCCCTG 
TGCCAGTATG CAGGTTGGGA GAACAGCATC TCAGCGTGGC AGAACCCAGT ACTGGACGAC 
AGATCCTTGC CTGCCTGGTA CAAATCTGCA CTGTTCAATG AACTATACTT CCTGGCTGAT 
GGAGGCACGG TGTGGCTGGA AGTTCCTGAA GACTCCCTAC CAGAGGAGCT GGGAGGGAGT 
ATGTACCAGC TGCGCCCTAT TCTGCAGGAC TATGGGCGAT TTGGCTATCT CGAAGGCCAG 
GAGTACCGCA TGTACAACAC ATACGATGTC CACTTCTACG CTTCTTTTGC TCTCGTCATG 
CTGTGGCCCA AACTTGAGCT CAGTCTTCAG TATGATATGG CTCTGGCAAC TTTCAAGGAG 
GACCTGACAC GGCGACGGTA CCTGATGAGT GGAGTAGTGG CGCCTGTGAA AAGGAGGAAC 
GTTATCCCTC ATGATATTGG GGATCCAGAT GATGAGCCAT GGCTCCGGGT CAACGCATAT 
TTGATCCATG ATACTGCTGA CTGGAAGGAC CTGAACCTGA AGTTTGTGTT GCAAGTTTAT 
CGGGACTATT ACCTGACAGG CGATCAAGGC TTCCTGAAGG ACATGTGGCC TGTGTGTCTG 
GCTGTGATGG AGTCCGAAAT GAAGTTTGAT AAGGACCAAG ATGGACTCAT TGAGAATGGA 
GGCTATGCAG ACCAGACCTA CGATGGATGG GTCACCACAG GCCCCAGTGC TTACTGCGGA 
GGGCTGTGGC TGGCAGCTGT GGCTGTGATG GTCCAGATGG CCGTTCTGTG TGGGGCCCAG 
GATGTCCAGG ATAAGTTTTC TTCCATTCTC TGCCGAGGCC GGGAAGCTTA TGAGAGATTG 
CTCTGGAACG GACGCTATTA CAACTACGAC AGCAGCTCCC AGCCACAGTC CCGCAGCGTC 
ATGTCTGACC AGTGTGCTGG GCAGTGGTTC CTGAGGGCCT GCGGCCTGGG AGAAGGAGAC 
ACTGAAGTAT TCCCTACCCT GCATGTTGTC CGTGCTCTCA AAACCATCTT TGAGCTCAAT 
GTCCAAGCCT TCGCAGGAGG AGCCATGGGG GCTGTGAATG GGATGCAGCC TCATGGTGTC 
CCTGATAGGT CCAGTGTGCA GTCTGATGAA GTTTGGGTGG GCGTGGTCTA TGGGCTGGCA 
GCCACCATGA TCCAAGAGGG CCTGACTTGG GAAGGTTTCC GGACAGCTGA AGGCTGTTAC 
CGCACTGTAT GGGAACGCCT GGGCCTGGCT TTCCAGACCC CAGAGGCATA CTGCCAGCAG 
CGAGTGTTCC GCTCCCTGGC CTACATGCGG CCCCTGAGCA TCTGGGCCAT GCAGCTGGCC 
CTTCAACAGC AGCAGCATAA AAAGAACAGC AGCCGGCCAG CAGTCACACA AGGCACAGCA 
CCCAGCACAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013109.2
CDS	1..2715
Translation	MVTCVPASEQIGCAERDSQIYSEDTGGTEAVRVTDCRSPEDSGPQNEPGYCNSEDSGQLMASYEGKARGYQVPPFGWRICLAHEFAEKRKPFQANNVSLSNLVKHFGMGLRYLKWWYRKTQVEKKTPFIDMFNSVPLRQIYGCPLGGIGGGTITRGWRGQFCRWQLNPGMYQHQTVIADQFIVCLRRDGRTVYQQVLSLELPSVLRSWNWGLCGYFAFYHALYPRAWTVYQLPGQNVTLTCRQITPILPHDYQDSSLPVGVFVWDVENEGDETLDVSIMFSMRNGLGGEDDAAGGLWNEPFRLEQDGTTVQGLLLHHPTPPNPYTMAVAARHTADTTVTYTTAFDPDSTGQQVWQDLLQDGQLDSPAGQSTPTQRGEGVAGAVCASSKLLPRGRCCLEFSLAWDMPRIMFGAKGQVHYRRYTRFFGSDGDVAPALSHYALCQYAGWENSISAWQNPVLDDRSLPAWYKSALFNELYFLADGGTVWLEVPEDSLPEELGGSMYQLRPILQDYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMALATFKEDLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGDQGFLKDMWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMVQMAVLCGAQDVQDKFSSILCRGREAYERLLWNGRYYNYDSSSQPQSRSVMSDQCAGQWFLRACGLGEGDTEVFPTLHVVRALKTIFELNVQAFAGGAMGAVNGMQPHGVPDRSSVQSDEVWVGVVYGLAATMIQEGLTWEGFRTAEGCYRTVWERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLALQQQQHKKNSSRPAVTQGTAPSTA

Target ORF information:

RefSeq Version	NM_001013091.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucosylceramidase beta 2 (Gba2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013091.2

ORF Insert Sequence:

ATGGTAACCT GCGTACCTGC CTCCGAGCAG ATCGGCTGTG CGGAAAGAGA TTCACAAATT 
TATAGTGAAG ATACTGGAGG CACTGAGGCT GTGCGGGTTA CAGACTGCCG GAGCCCGGAG 
GACAGTGGAC CCCAGAATGA GCCAGGCTAC TGCAATTCAG AGGACTCTGG GCAGCTGATG 
GCCTCCTACG AGGGTAAAGC TAGGGGCTAC CAGGTGCCTC CTTTTGGCTG GCGGATTTGC 
TTGGCTCACG AGTTTGCAGA GAAGAGGAAA CCTTTTCAGG CTAACAACGT CTCTCTCAGT 
AATTTGGTAA AGCATTTCGG TATGGGCTTG AGGTACTTGA AGTGGTGGTA CCGGAAGACG 
CAGGTGGAAA AGAAGACCCC TTTCATCGAC ATGTTCAATT CTGTACCCCT GAGACAGATC 
TACGGTTGCC CCTTGGGTGG CATTGGAGGA GGCACCATCA CCCGGGGCTG GAGAGGCCAG 
TTCTGTCGTT GGCAGCTTAA TCCTGGAATG TACCAGCACC AGACAGTCAT TGCAGACCAA 
TTTATAGTAT GCTTGCGTCG AGATGGGCGG ACTGTGTATC AGCAAGTCCT GTCCTTGGAG 
CTTCCGAGTG TCCTGCGCAG CTGGAACTGG GGCCTGTGTG GTTATTTTGC TTTCTACCAC 
GCCCTCTATC CCCGAGCCTG GACAGTCTAT CAGCTTCCTG GCCAGAATGT CACCCTCACC 
TGTCGCCAGA TCACACCTAT CTTGCCTCAT GATTACCAGG ACAGCAGCCT CCCCGTAGGA 
GTGTTTGTGT GGGATGTAGA GAATGAAGGA GATGAGACTC TGGATGTGTC CATCATGTTC 
TCCATGCGGA ATGGACTAGG AGGTGAAGAT GATGCTGCCG GAGGTTTGTG GAACGAGCCC 
TTCCGTCTGG AACAGGATGG GACGACTGTA CAGGGGCTAC TCCTGCATCA TCCAACCCCT 
CCGAACCCCT ACACCATGGC TGTGGCTGCG CGACACACGG CAGATACCAC AGTAACCTAC 
ACCACAGCCT TTGACCCCGA CAGCACTGGG CAGCAGGTGT GGCAGGACCT ACTTCAAGAT 
GGACAGCTGG ACTCCCCTGC TGGCCAGAGC ACCCCGACAC AGAGAGGAGA GGGTGTCGCT 
GGGGCTGTAT GTGCCTCCAG CAAGCTGCTG CCACGAGGCC GGTGCTGCTT GGAGTTCTCA 
CTGGCTTGGG ATATGCCTAG GATCATGTTT GGAGCCAAAG GCCAAGTCCA CTACAGGCGG 
TATACACGGT TCTTTGGTTC AGATGGTGAT GTGGCGCCTG CCCTGAGCCA CTATGCCCTG 
TGCCAGTATG CAGGTTGGGA GAACAGCATC TCAGCGTGGC AGAACCCAGT ACTGGACGAC 
AGATCCTTGC CTGCCTGGTA CAAATCTGCA CTGTTCAATG AACTATACTT CCTGGCTGAT 
GGAGGCACGG TGTGGCTGGA AGTTCCTGAA GACTCCCTAC CAGAGGAGCT GGGAGGGAGT 
ATGTACCAGC TGCGCCCTAT TCTGCAGGAC TATGGGCGAT TTGGCTATCT CGAAGGCCAG 
GAGTACCGCA TGTACAACAC ATACGATGTC CACTTCTACG CTTCTTTTGC TCTCGTCATG 
CTGTGGCCCA AACTTGAGCT CAGTCTTCAG TATGATATGG CTCTGGCAAC TTTCAAGGAG 
GACCTGACAC GGCGACGGTA CCTGATGAGT GGAGTAGTGG CGCCTGTGAA AAGGAGGAAC 
GTTATCCCTC ATGATATTGG GGATCCAGAT GATGAGCCAT GGCTCCGGGT CAACGCATAT 
TTGATCCATG ATACTGCTGA CTGGAAGGAC CTGAACCTGA AGTTTGTGTT GCAAGTTTAT 
CGGGACTATT ACCTGACAGG CGATCAAGGC TTCCTGAAGG ACATGTGGCC TGTGTGTCTG 
GCTGTGATGG AGTCCGAAAT GAAGTTTGAT AAGGACCAAG ATGGACTCAT TGAGAATGGA 
GGCTATGCAG ACCAGACCTA CGATGGATGG GTCACCACAG GCCCCAGTGC TTACTGCGGA 
GGGCTGTGGC TGGCAGCTGT GGCTGTGATG GTCCAGATGG CCGTTCTGTG TGGGGCCCAG 
GATGTCCAGG ATAAGTTTTC TTCCATTCTC TGCCGAGGCC GGGAAGCTTA TGAGAGATTG 
CTCTGGAACG GACGCTATTA CAACTACGAC AGCAGCTCCC AGCCACAGTC CCGCAGCGTC 
ATGTCTGACC AGTGTGCTGG GCAGTGGTTC CTGAGGGCCT GCGGCCTGGG AGAAGGAGAC 
ACTGAAGTAT TCCCTACCCT GCATGTTGTC CGTGCTCTCA AAACCATCTT TGAGCTCAAT 
GTCCAAGCCT TCGCAGGAGG AGCCATGGGG GCTGTGAATG GGATGCAGCC TCATGGTGTC 
CCTGATAGGT CCAGTGTGCA GTCTGATGAA GTTTGGGTGG GCGTGGTCTA TGGGCTGGCA 
GCCACCATGA TCCAAGAGGG CCTGACTTGG GAAGGTTTCC GGACAGCTGA AGGCTGTTAC 
CGCACTGTAT GGGAACGCCT GGGCCTGGCT TTCCAGACCC CAGAGGCATA CTGCCAGCAG 
CGAGTGTTCC GCTCCCTGGC CTACATGCGG CCCCTGAGCA TCTGGGCCAT GCAGCTGGCC 
CTTCAACAGC AGCAGCATAA AAAGAACAGC AGCCGGCCAG CAGTCACACA AGGCACAGCA 
CCCAGCACAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glucosylated cholesterol in mammalian cells and tissues: formation and degradation by multiple cellular ?-glucosidases.
Journal of lipid research57(3)451-63(2016 Mar)
Marques AR,Mirzaian M,Akiyama H,Wisse P,Ferraz MJ,Gaspar P,Ghauharali-van der Vlugt K,Meijer R,Giraldo P,Alfonso P,Ir?n P,Dahl M,Karlsson S,Pavlova EV,Cox TM,Scheij S,Verhoek M,Ottenhoff R,van Roomen CP,Pannu NS,van Eijk M,Dekker N,Boot RG,Overkleeft HS,Blommaart E,Hirabayashi Y,Aerts JM

Accumulation of glucosylceramide in the absence of the beta-glucosidase GBA2 alters cytoskeletal dynamics.
PLoS genetics11(3)e1005063(2015 Mar)
Raju D,Schonauer S,Hamzeh H,Flynn KC,Bradke F,Vom Dorp K,D?rmann P,Yildiz Y,Tr?tschel C,Poetsch A,Breiden B,Sandhoff K,K?rschen HG,Wachten D

The non-lysosomal ?-glucosidase GBA2 is a non-integral membrane-associated protein at the endoplasmic reticulum (ER) and Golgi.
The Journal of biological chemistry288(5)3381-93(2013 Feb)
K?rschen HG,Yildiz Y,Raju DN,Schonauer S,B?nigk W,Jansen V,Kremmer E,Kaupp UB,Wachten D

Loss of function of glucocerebrosidase GBA2 is responsible for motor neuron defects in hereditary spastic paraplegia.
American journal of human genetics92(2)238-44(2013 Feb)
Martin E,Sch?le R,Smets K,Rastetter A,Boukhris A,Loureiro JL,Gonzalez MA,Mundwiller E,Deconinck T,Wessner M,Jornea L,Oteyza AC,Durr A,Martin JJ,Sch?ls L,Mhiri C,Lamari F,Z?chner S,De Jonghe P,Kabashi E,Brice A,Stevanin G

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Gba2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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