Slc25a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc25a1
Entrez Gene ID	29743
Full Name	solute carrier family 25 member 1
Synonyms	Cic,Ctp,Slc20a3
General protein information	Preferred Names solute carrier family 25 member 1 Names tricarboxylate transport protein, mitochondrial citrate transport protein citrate transporter) member 1 mitochondrial tricarboxylate carrier solute carrier family 25 (mitochondrial carrier, citrate transporter), member 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23
Summary	This gene encodes a member of the mitochondrial carrier family. The encoded protein is responsible for the movement of tricarboxylates, such as citrate, across the inner mitochondrial membrane. It is essential to the bioenergetics of hepatic cells, supplying the cytoplasm with a carbon source for fatty acid and sterol biosynthesis, and NAD+ for the glycolytic pathway. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017307.3	NP_059003.1	tricarboxylate transport protein, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SLC25A1	XP_001165281.1
Mus musculus (house mouse)	Slc25a1	NP_694790.1
Danio rerio (zebrafish)	slc25a1a	NP_956901.1
Caenorhabditis elegans (roundworm)	K11H3.3	NP_499187.1
Eremothecium gossypii	AGOS_AGL064W	NP_986602.1
Schizosaccharomyces pombe (fission yeast)	SPAC19G12.05	NP_594420.1
Rattus norvegicus (Norway rat)	Slc25a1	NP_059003.1
Magnaporthe oryzae (rice blast fungus)	MGG_03479	XP_003716446.1
Xenopus tropicalis (tropical clawed frog)	slc25a1	NP_989391.1
	SLC25A1	XP_001112697.2
Bos taurus (cattle)	SLC25A1	XP_005218363.1
Neurospora crassa	NCU05075	XP_956064.2
Canis lupus familiaris (dog)	SLC25A1	XP_850441.2
Gallus gallus (chicken)	SLC25A1	XP_415059.2
Drosophila melanogaster (fruit fly)	sea	NP_001027175.1

Gene Ontology
Bibliography

Related articles in PubMed

Pathogenic mutations of the human mitochondrial citrate carrier SLC25A1 lead to impaired citrate export required for lipid, dolichol, ubiquinone and sterol synthesis.
Majd H, King MS, Smith AC, Kunji ERS
Biochimica et biophysica acta1859(1)1-7(2018 Jan)

Deficiency in SLC25A1, encoding the mitochondrial citrate carrier, causes combined D-2- and L-2-hydroxyglutaric aciduria.
Nota B, Struys EA, Pop A, Jansen EE, Fernandez Ojeda MR, Kanhai WA, Kranendijk M, van Dooren SJ, Bevova MR, Sistermans EA, Nieuwint AW, Barth M, Ben-Omran T, Hoffmann GF, de Lonlay P, McDonald MT, Meberg A, Muntau AC, Nuoffer JM, Parini R, Read MH, Renneberg A, Santer R, Strahleck T, van Schaftingen E, van der Knaap MS, Jakobs C, Salomons GS
American journal of human genetics92(4)627-31(2013 Apr)

Citrate carrier promoter is target of peroxisome proliferator-activated receptor alpha and gamma in hepatocytes and adipocytes.
Damiano F, Gnoni GV, Siculella L
The international journal of biochemistry & cell biology44(4)659-68(2012 Apr)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

binding of PPRE of CiC promoter by PPARalpha and PPARgammain vivo
Title: Citrate carrier promoter is target of peroxisome proliferator-activated receptor alpha and gamma in hepatocytes and adipocytes.

that export of citrate and/or isocitrate from the mitochondria to the cytosol through Slc25a1 is an important step in control of glucose-stimulated insulin secretion
Title: The mitochondrial citrate/isocitrate carrier plays a regulatory role in glucose-stimulated insulin secretion.

Data show that under conditions of increased ionic strength, the citrate carrier precursor protein presequence binds to Tom20 and accumulates at the mitochondrial surface.
Title: Import of rat mitochondrial citrate carrier (CIC) at increasing salt concentrations promotes presequence binding to import receptor Tom20 and inhibits membrane translocation.

Different dietary fatty acids have dissimilar effects on activity and gene expression of Ctp.
Title: Different dietary fatty acids have dissimilar effects on activity and gene expression of mitochondrial tricarboxylate carrier in rat liver.

administration of n-6 PUFAs to rats leads to changes not only in the transcriptional rate of the tricarboxylate (citrate) carrier gene but also in the processing of the nuclear precursor for TCC RNA.
Title: n-6 PUFAs downregulate expression of the tricarboxylate carrier in rat liver by transcriptional and posttranscriptional mechanisms.

The following Slc25a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc25a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13008	NM_017307.3 Latest version!	Rattus norvegicus solute carrier family 25 member 1 (Slc25a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc25a1 ( NM_017307.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_059003.1 Rattus norvegicus solute carrier family 25 member 1 (Slc25a1), mRNA.

Price & Availability↑

CloneID	ORa13008
Clone ID Related Accession (Same CDS sequence)	NM_017307.3
Accession Version	NM_017307.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	tricarboxylate transport protein, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB758644.1 and BC100077.1. On Oct 4, 2005 this sequence version replaced NM_017307.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC100077.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGC CTCGCGCACC CCGCGCTCTG ACGGCTGCCG CTCCCGGGTC CGGGAAAGCC 
AAACTGACGC ACCCCGGGAA GGCGATCCTA GCAGGCGGCC TGGCTGGAGG CATCGAAATC 
TGCATCACCT TCCCGACCGA ATACGTGAAG ACTCAACTAC AGCTAGATGA ACGCGCGAAC 
CCACCGCGGT ACCGGGGCAT TGGGGACTGC GTGCGGCAAA CTGTCCGCAG CCATGGCGTC 
CTGGGCCTGT ACCGCGGCCT CAGCTCCTTG CTCTACGGCT CCATCCCCAA GGCGGCTGTC 
AGGTTTGGGA TGTTCGAGTT CCTCAGCAAC CACATGCGGG ATGCCCAAGG TAGACTGGAC 
AGCAGGAGAG GACTGCTGTG CGGTCTGGGT GCCGGCGTGG CAGAGGCAGT GGTAGTCGTG 
TGCCCCATGG AGACCGTGAA GGTGAAATTC ATCCATGACC AGACTTCCTC CAATCCAAAG 
TACCGAGGAT TTTTCCATGG GGTTAGAGAG ATTGTTCGGG AACAAGGGCT AAAGGGGACA 
TACCAAGGCC TCACAGCTAC TGTACTGAAG CAGGGCTCAA ACCAAGCCAT CCGATTCTTC 
GTCATGACCT CCCTACGCAA CTGGTACCAA GGAGACAACC CCAACAAGCC TATGAACCCA 
CTGATCACCG GGGTCTTTGG AGCTGTCGCA GGTGCAGCCA GTGTCTTTGG GAACACACCC 
TTGGATGTGA TCAAGACCAG GATGCAGGGC CTGGAGGCAC ACAAATACCG GAACACCCTG 
GACTGTGGAG TGCAGATCCT GAAGAATGAG GGGCCCAAGG CATTCTACAA GGGCACCGTT 
CCCCGGCTGG GCCGAGTCTG CCTGGACGTG GCCATCGTAT TCGTCATCTA TGATGAAGTG 
GTGAAGCTGC TCAATAAAGT GTGGAAGACG GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059003.1
CDS	115..1050
Translation	MAAPRAPRALTAAAPGSGKAKLTHPGKAILAGGLAGGIEICITFPTEYVKTQLQLDERANPPRYRGIGDCVRQTVRSHGVLGLYRGLSSLLYGSIPKAAVRFGMFEFLSNHMRDAQGRLDSRRGLLCGLGAGVAEAVVVVCPMETVKVKFIHDQTSSNPKYRGFFHGVREIVREQGLKGTYQGLTATVLKQGSNQAIRFFVMTSLRNWYQGDNPNKPMNPLITGVFGAVAGAASVFGNTPLDVIKTRMQGLEAHKYRNTLDCGVQILKNEGPKAFYKGTVPRLGRVCLDVAIVFVIYDEVVKLLNKVWKTD

Target ORF information:

RefSeq Version	NM_017307.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 25 member 1 (Slc25a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017307.3

ORF Insert Sequence:

ATGGCTGCGC CTCGCGCACC CCGCGCTCTG ACGGCTGCCG CTCCCGGGTC CGGGAAAGCC 
AAACTGACGC ACCCCGGGAA GGCGATCCTA GCAGGCGGCC TGGCTGGAGG CATCGAAATC 
TGCATCACCT TCCCGACCGA ATACGTGAAG ACTCAACTAC AGCTAGATGA ACGCGCGAAC 
CCACCGCGGT ACCGGGGCAT TGGGGACTGC GTGCGGCAAA CTGTCCGCAG CCATGGCGTC 
CTGGGCCTGT ACCGCGGCCT CAGCTCCTTG CTCTACGGCT CCATCCCCAA GGCGGCTGTC 
AGGTTTGGGA TGTTCGAGTT CCTCAGCAAC CACATGCGGG ATGCCCAAGG TAGACTGGAC 
AGCAGGAGAG GACTGCTGTG CGGTCTGGGT GCCGGCGTGG CAGAGGCAGT GGTAGTCGTG 
TGCCCCATGG AGACCGTGAA GGTGAAATTC ATCCATGACC AGACTTCCTC CAATCCAAAG 
TACCGAGGAT TTTTCCATGG GGTTAGAGAG ATTGTTCGGG AACAAGGGCT AAAGGGGACA 
TACCAAGGCC TCACAGCTAC TGTACTGAAG CAGGGCTCAA ACCAAGCCAT CCGATTCTTC 
GTCATGACCT CCCTACGCAA CTGGTACCAA GGAGACAACC CCAACAAGCC TATGAACCCA 
CTGATCACCG GGGTCTTTGG AGCTGTCGCA GGTGCAGCCA GTGTCTTTGG GAACACACCC 
TTGGATGTGA TCAAGACCAG GATGCAGGGC CTGGAGGCAC ACAAATACCG GAACACCCTG 
GACTGTGGAG TGCAGATCCT GAAGAATGAG GGGCCCAAGG CATTCTACAA GGGCACCGTT 
CCCCGGCTGG GCCGAGTCTG CCTGGACGTG GCCATCGTAT TCGTCATCTA TGATGAAGTG 
GTGAAGCTGC TCAATAAAGT GTGGAAGACG GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pathogenic mutations of the human mitochondrial citrate carrier SLC25A1 lead to impaired citrate export required for lipid, dolichol, ubiquinone and sterol synthesis.
Biochimica et biophysica acta1859(1)1-7(2018 Jan)
Majd H,King MS,Smith AC,Kunji ERS

Deficiency in SLC25A1, encoding the mitochondrial citrate carrier, causes combined D-2- and L-2-hydroxyglutaric aciduria.
American journal of human genetics92(4)627-31(2013 Apr)
Nota B,Struys EA,Pop A,Jansen EE,Fernandez Ojeda MR,Kanhai WA,Kranendijk M,van Dooren SJ,Bevova MR,Sistermans EA,Nieuwint AW,Barth M,Ben-Omran T,Hoffmann GF,de Lonlay P,McDonald MT,Meberg A,Muntau AC,Nuoffer JM,Parini R,Read MH,Renneberg A,Santer R,Strahleck T,van Schaftingen E,van der Knaap MS,Jakobs C,Salomons GS

Citrate carrier promoter is target of peroxisome proliferator-activated receptor alpha and gamma in hepatocytes and adipocytes.
The international journal of biochemistry & cell biology44(4)659-68(2012 Apr)
Damiano F,Gnoni GV,Siculella L

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

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Slc25a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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