Kcnj5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnj5
Entrez Gene ID	29713
Full Name	potassium voltage-gated channel subfamily J member 5
General protein information	Preferred Names potassium voltage-gated channel subfamily J member 5 Names G protein-activated inward rectifier potassium channel 4 CIR GIRK-4 GIRK4 KATP-1 cardiac inward rectifier heart KATP channel inward rectifier K(+) channel Kir3.4 potassium channel, inwardly rectifying subfamily J member 5 potassium inwardly-rectifying channel, subfamily J, member 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q21
Summary	inwardly-rectifying potassium channel which is controlled by G proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017297.2	NP_058993.1	G protein-activated inward rectifier potassium channel 4

Pathways from BioSystems

Homologies

	KCNJ5	XP_001113260.1
Canis lupus familiaris (dog)	KCNJ5	XP_546402.2
Pan troglodytes (chimpanzee)	KCNJ5	XP_508857.3
Rattus norvegicus (Norway rat)	Kcnj5	NP_058993.1
Bos taurus (cattle)	KCNJ5	XP_005226888.1
Mus musculus (house mouse)	Kcnj5	NP_034735.3
Danio rerio (zebrafish)	kcnj5	XP_700619.4
Homo sapiens (human)	KCNJ5	NP_000881.3
Gallus gallus (chicken)	KCNJ5	XP_417864.2
Xenopus tropicalis (tropical clawed frog)	kcnj5	NP_001016901.1

Gene Ontology
Bibliography

Related articles in PubMed

Constitutively Active Acetylcholine-Dependent Potassium Current Increases Atrial Defibrillation Threshold by Favoring Post-Shock Re-Initiation.
Bingen BO, Askar SF, Neshati Z, Feola I, Panfilov AV, de Vries AA, Pijnappels DA
Scientific reports515187(2015 Oct)

Effect of small interference RNA on the acetylcholine-sensitive potassium channel in H9c2 cells.
He F, Zhao J, Li L, Guo R
Annals of clinical and laboratory science45(1)58-63(2015)

G-protein-coupled inward rectifier potassium current contributes to ventricular repolarization.
Liang B, Nissen JD, Laursen M, Wang X, Skibsbye L, Hearing MC, Andersen MN, Rasmussen HB, Wickman K, Grunnet M, Olesen SP, Jespersen T
Cardiovascular research101(1)175-84(2014 Jan)

G-protein-gated inwardly rectifying K+ channel 4 (GIRK4) immunoreactivity in chemically defined neurons of the hypothalamic arcuate nucleus that control body weight.
Kloukina V, Herzer S, Karlsson N, Perez M, Daraio T, Meister B
Journal of chemical neuroanatomy44(1)14-23(2012 May)

Estrogen regulation of the dopamine-activated GIRK channel in pituitary lactotrophs: implications for regulation of prolactin release during the estrous cycle.
Christensen HR, Zeng Q, Murawsky MK, Gregerson KA
American journal of physiology. Regulatory, integrative and comparative physiology301(3)R746-56(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Blocking IK,ACh-c by tertiapin significantly decreased DFT, which correlated with a significant increase in wavelength during reentry. Genetic knockdown experiments with Kcnj5-specific shRNA to modulate IK,ACh-c, yielded similar results
Title: Constitutively Active Acetylcholine-Dependent Potassium Current Increases Atrial Defibrillation Threshold by Favoring Post-Shock Re-Initiation.

The KCNJ5 gene and Kir3.4 protein were measured, and acetylcholine-sensitive potassium channel currents were recorded by patch-clamp.
Title: Effect of small interference RNA on the acetylcholine-sensitive potassium channel in H9c2 cells.

localized in intercalated discs in rat ventricle, whereas it was expressed in intercalated discs and outer sarcolemmas in rat atrium
Title: G-protein-coupled inward rectifier potassium current contributes to ventricular repolarization.

the GIRK4 channel protein plays a role in regulating membrane excitability in chemically defined neurons of the arcuate nucleus that control body weight
Title: G-protein-gated inwardly rectifying K+ channel 4 (GIRK4) immunoreactivity in chemically defined neurons of the hypothalamic arcuate nucleus that control body weight.

Estradiol regulates dopamine-activataed GIRK channel activity in pituitary lactotrophs, regulating prolactin release during the estrous cycle.
Title: Estrogen regulation of the dopamine-activated GIRK channel in pituitary lactotrophs: implications for regulation of prolactin release during the estrous cycle.

The following Kcnj5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnj5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42707	NM_017297.2 Latest version!	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 5 (Kcnj5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnj5 ( NM_017297.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058993.1 Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 5 (Kcnj5), mRNA.

Price & Availability↑

CloneID	ORa42707
Clone ID Related Accession (Same CDS sequence)	NM_017297.2
Accession Version	NM_017297.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Rattus norvegicus(Norway rat)
Product	G protein-activated inward rectifier potassium channel 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC087022.1. On Dec 26, 2004 this sequence version replaced NM_017297.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC087022.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCGGTG ATTCTAGGAA TGCTATGAAT CAAGACATGG AGATAGGAGT CACCTCCCAG 
GACCACAAGA AGATCCCCAA ACAGGCTCGG GATTACATCC CCATTGCCAC AGACCGCACT 
CGCCTGCTGC CGGAAGGCAA GAAGCCACGC CAGCGCTACA TGGAGAAGAC CGGCAAGTGT 
AACGTGCACC ATGGCAATGT TCAGGAAACC TACCGCTACC TAAGTGACCT CTTCACCACC 
CTGGTGGACC TCAAATGGCG CTTCAACCTT CTGGTCTTCA CCATGGTCTA CACCATTACT 
TGGCTATTCT TTGGCTTCAT CTGGTGGCTC ATTGCTTATG TCCGAGGTGA TCTGGACCAC 
GTGGGTGACC AAGAGTGGAT CCCTTGTGTT GAAAACCTTA GTGGCTTTGT GTCTGCTTTC 
CTGTTCTCCA TTGAGACAGA AACAACCATT GGGTATGGCT TCAGAGTCAT TACAGAGAAG 
TGTCCAGAGG GGATCATTCT CCTTCTAGTG CAGGCCATCC TGGGCTCTAT TGTTAATGCC 
TTCATGGTGG GTTGCATGTT TGTAAAGATC AGCCAGCCAA AGAAGAGAGC AGAGACCCTC 
ATGTTCTCCA ACAATGCTGT CATCTCCATG CGGGATGAGA AGCTGTGCCT CATGTTCCGG 
GTAGGGGACC TCCGAAACTC CCATATCGTG GAGGCCTCCA TCCGCGCCAA GCTTATCAAG 
TCCCGGCAGA CCAAAGAAGG GGAATTCATC CCCTTGAACC AGACCGACAT TAACGTGGGC 
TTTGACACTG GTGACGACCG CCTCTTCCTG GTGTCCCCCC TCATCATCTC CCATGAGATC 
AATGAGAAGA GCCCTTTCTG GGAGATGTCT CGTGCTCAAC TGGAGCAGGA AGAGTTCGAG 
GTTGTGGTCA TACTAGAAGG GATGGTAGAA GCCACAGGCA TGACTTGCCA AGCACGGAGC 
TCTTACATGG ATACAGAGGT GCTCTGGGGT CACCGATTCA CACCAGTCCT CACCTTGGAA 
AAGGGCTTCT ATGAGGTGGA CTACAACACT TTCCACGACA CCTATGAGAC CAACACACCC 
AGCTGCTGTG CCAAGGAGCT GGCAGAAATG AAGAGGAATG GTCAGCTCCT CCAGTCCTTG 
CCCAGTCCTC CTTTGCTTGG GGGCTGCGCT GAGGCTGAGA AAGAAGCAGA GGCTGAGCAC 
GATGAGGAGG AGGAACCCAA TGGACTGAGT GTGTCCCGGG CAACAAGGGG CTCAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058993.1
CDS	177..1436
Translation	MAGDSRNAMNQDMEIGVTSQDHKKIPKQARDYIPIATDRTRLLPEGKKPRQRYMEKTGKCNVHHGNVQETYRYLSDLFTTLVDLKWRFNLLVFTMVYTITWLFFGFIWWLIAYVRGDLDHVGDQEWIPCVENLSGFVSAFLFSIETETTIGYGFRVITEKCPEGIILLLVQAILGSIVNAFMVGCMFVKISQPKKRAETLMFSNNAVISMRDEKLCLMFRVGDLRNSHIVEASIRAKLIKSRQTKEGEFIPLNQTDINVGFDTGDDRLFLVSPLIISHEINEKSPFWEMSRAQLEQEEFEVVVILEGMVEATGMTCQARSSYMDTEVLWGHRFTPVLTLEKGFYEVDYNTFHDTYETNTPSCCAKELAEMKRNGQLLQSLPSPPLLGGCAEAEKEAEAEHDEEEEPNGLSVSRATRGSM

Target ORF information:

RefSeq Version	NM_017297.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 5 (Kcnj5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017297.2

ORF Insert Sequence:

ATGGCCGGTG ATTCTAGGAA TGCTATGAAT CAAGACATGG AGATAGGAGT CACCTCCCAG 
GACCACAAGA AGATCCCCAA ACAGGCTCGG GATTACATCC CCATTGCCAC AGACCGCACT 
CGCCTGCTGC CGGAAGGCAA GAAGCCACGC CAGCGCTACA TGGAGAAGAC CGGCAAGTGT 
AACGTGCACC ATGGCAATGT TCAGGAAACC TACCGCTACC TAAGTGACCT CTTCACCACC 
CTGGTGGACC TCAAATGGCG CTTCAACCTT CTGGTCTTCA CCATGGTCTA CACCATTACT 
TGGCTATTCT TTGGCTTCAT CTGGTGGCTC ATTGCTTATG TCCGAGGTGA TCTGGACCAC 
GTGGGTGACC AAGAGTGGAT CCCTTGTGTT GAAAACCTTA GTGGCTTTGT GTCTGCTTTC 
CTGTTCTCCA TTGAGACAGA AACAACCATT GGGTATGGCT TCAGAGTCAT TACAGAGAAG 
TGTCCAGAGG GGATCATTCT CCTTCTAGTG CAGGCCATCC TGGGCTCTAT TGTTAATGCC 
TTCATGGTGG GTTGCATGTT TGTAAAGATC AGCCAGCCAA AGAAGAGAGC AGAGACCCTC 
ATGTTCTCCA ACAATGCTGT CATCTCCATG CGGGATGAGA AGCTGTGCCT CATGTTCCGG 
GTAGGGGACC TCCGAAACTC CCATATCGTG GAGGCCTCCA TCCGCGCCAA GCTTATCAAG 
TCCCGGCAGA CCAAAGAAGG GGAATTCATC CCCTTGAACC AGACCGACAT TAACGTGGGC 
TTTGACACTG GTGACGACCG CCTCTTCCTG GTGTCCCCCC TCATCATCTC CCATGAGATC 
AATGAGAAGA GCCCTTTCTG GGAGATGTCT CGTGCTCAAC TGGAGCAGGA AGAGTTCGAG 
GTTGTGGTCA TACTAGAAGG GATGGTAGAA GCCACAGGCA TGACTTGCCA AGCACGGAGC 
TCTTACATGG ATACAGAGGT GCTCTGGGGT CACCGATTCA CACCAGTCCT CACCTTGGAA 
AAGGGCTTCT ATGAGGTGGA CTACAACACT TTCCACGACA CCTATGAGAC CAACACACCC 
AGCTGCTGTG CCAAGGAGCT GGCAGAAATG AAGAGGAATG GTCAGCTCCT CCAGTCCTTG 
CCCAGTCCTC CTTTGCTTGG GGGCTGCGCT GAGGCTGAGA AAGAAGCAGA GGCTGAGCAC 
GATGAGGAGG AGGAACCCAA TGGACTGAGT GTGTCCCGGG CAACAAGGGG CTCAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Constitutively Active Acetylcholine-Dependent Potassium Current Increases Atrial Defibrillation Threshold by Favoring Post-Shock Re-Initiation.
Scientific reports515187(2015 Oct)
Bingen BO,Askar SF,Neshati Z,Feola I,Panfilov AV,de Vries AA,Pijnappels DA

Effect of small interference RNA on the acetylcholine-sensitive potassium channel in H9c2 cells.
Annals of clinical and laboratory science45(1)58-63(2015)
He F,Zhao J,Li L,Guo R

G-protein-coupled inward rectifier potassium current contributes to ventricular repolarization.
Cardiovascular research101(1)175-84(2014 Jan)
Liang B,Nissen JD,Laursen M,Wang X,Skibsbye L,Hearing MC,Andersen MN,Rasmussen HB,Wickman K,Grunnet M,Olesen SP,Jespersen T

G-protein-gated inwardly rectifying K+ channel 4 (GIRK4) immunoreactivity in chemically defined neurons of the hypothalamic arcuate nucleus that control body weight.
Journal of chemical neuroanatomy44(1)14-23(2012 May)
Kloukina V,Herzer S,Karlsson N,Perez M,Daraio T,Meister B

Estrogen regulation of the dopamine-activated GIRK channel in pituitary lactotrophs: implications for regulation of prolactin release during the estrous cycle.
American journal of physiology. Regulatory, integrative and comparative physiology301(3)R746-56(2011 Sep)
Christensen HR,Zeng Q,Murawsky MK,Gregerson KA

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Kcnj5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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