Prkar2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkar2a
Entrez Gene ID	29699
Full Name	protein kinase cAMP-dependent type 2 regulatory subunit alpha
General protein information	Preferred Names protein kinase cAMP-dependent type 2 regulatory subunit alpha Names cAMP-dependent protein kinase type II-alpha regulatory subunit protein kinase, cAMP dependent regulatory, type II alpha protein kinase, cAMP-dependent, regulatory subunit type II alpha protein kinase, cAMP-dependent, regulatory, type 2, alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	regulatory subunit for cAMP dependent protein kinase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006243713.3	XP_006243775.1	cAMP-dependent protein kinase type II-alpha regulatory subunit isoform X1
NM_019264.2	NP_062137.1	cAMP-dependent protein kinase type II-alpha regulatory subunit

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PRKAR2A	XP_003432928.1
Bos taurus (cattle)	PRKAR2A	NP_001178296.1
Gallus gallus (chicken)	LOC100858827	XP_003642054.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004940	XP_315029.3
Eremothecium gossypii	AGOS_ADL099C	NP_983997.1
Mus musculus (house mouse)	Prkar2a	NP_032950.1
Rattus norvegicus (Norway rat)	Prkar2a	NP_062137.1
Drosophila melanogaster (fruit fly)	Pka-R2	NP_724860.2
Neurospora crassa	NCU01166	XP_961532.1
Homo sapiens (human)	PRKAR2A	NP_004148.1
Kluyveromyces lactis	KLLA0E04181g	XP_454132.1
Xenopus tropicalis (tropical clawed frog)	prkar2a	XP_002933104.1
Danio rerio (zebrafish)	prkar2aa	NP_001070838.2
Saccharomyces cerevisiae (baker's yeast)	BCY1	NP_012231.1
	LOC708981	XP_002808369.1
Schizosaccharomyces pombe (fission yeast)	cgs1	NP_594274.1
Magnaporthe oryzae (rice blast fungus)	MGG_07335	XP_003715572.1

Gene Ontology
Bibliography

Related articles in PubMed

A systematic evaluation of protein kinase A-A-kinase anchoring protein interaction motifs.
Burgers PP, van der Heyden MA, Kok B, Heck AJ, Scholten A
Biochemistry54(1)11-21(2015 Jan)

Cytoplasmic anchoring of cAMP-dependent protein kinase (PKA) by A-kinase anchor proteins (AKAPs) is required for meiotic arrest of porcine full-grown and growing oocytes.
Nishimura T, Fujii W, Sugiura K, Naito K
Biology of reproduction90(3)58(2014 Mar)

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Gr?nborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, Jahn O, Brose N, Herzog E
The EMBO journal33(2)157-70(2014 Jan)

A-kinase anchor protein 1 (AKAP1) regulates cAMP-dependent protein kinase (PKA) localization and is involved in meiotic maturation of porcine oocytes.
Nishimura T, Sugiura K, Naito K
Biology of reproduction88(4)85(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The crystal structure of the helical motif from D-AKAP2 (Akap10), a dual-specific AKAP, bound to the RIIalpha D/D domain from Prkar2a, was analysed.
Title: A dynamic mechanism for AKAP binding to RII isoforms of cAMP-dependent protein kinase.

The following Prkar2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkar2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04211	NM_019264.2 Latest version!	Rattus norvegicus protein kinase cAMP-dependent type II regulatory subunit alpha (Prkar2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa31546	XM_006243713.3 Latest version!	Rattus norvegicus protein kinase cAMP-dependent type 2 regulatory subunit alpha (Prkar2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkar2a ( NM_019264.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062137.1 Rattus norvegicus protein kinase cAMP-dependent type II regulatory subunit alpha (Prkar2a), mRNA.

Price & Availability↑

CloneID	ORa04211
Clone ID Related Accession (Same CDS sequence)	NM_019264.2
Accession Version	NM_019264.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase type II-alpha regulatory subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF533978.1. On Aug 31, 2012 this sequence version replaced NM_019264.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF533978.1, J02934.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCACA TCCAGATCCC ACCGGGGCTC ACGGAGCTGC TGCAGGGCTA CACCGTGGAG 
GTGCTTCGGC AGCAGCCGCC CGACCTCGTC GACTTCGCGG TGGAGTACTT CACACGCCTG 
CGCGAGGCCC GCCGCCAGGA ATCAGACTCG TTCATCGCCC CCCCGACGAC CTTTCACGCG 
CAGGAGTCCA GCGGGGTCCC CGTCATCGAG GAGGACGGGG AGAGTGAATC GGACTCGGAC 
GATGAGGATC TGGAAGTTCC GATTCCCAGC AAATTTACTA GACGAGTATC AGTCTGTGCA 
GAAACGTTTA ACCCTGATGA AGAAGAAGAT AATGATCCAA GGGTGGTTCA CCCCAAAACC 
GACGAGCAGA GGTACAGACT TCAGGAAGCC TGTAAAGACA TTCTGCTTTT CAAAAACCTG 
GATCAGGAAC AGCTTTCTCA AGTTCTGGAC GCCATGTTTG AAAAGATAGT CAAAACTGAC 
GAGCATGTCA TTGACCAAGG AGATGATGGA GACAACTTTT ATGTCATAGA AAGGGGAACC 
TATGACATTT TAGTAACAAA AGATAATCAA ACACGATCTG TTGGTCAGTA TGACAACCGT 
GGCAGTTTTG GAGAACTAGC CCTGATGTAC AATACCCCGA GAGCTGCTAC CATTGTGGCC 
ACCTCAGACG GCTCCCTTTG GGGATTGGAC CGGGTGACTT TTAGGAGAAT CATAGTGAAG 
AACAATGCAA AGAAGAGGAA GATGTTCGAA TCGTTTATTG AGTCTGTACC GCTCTTTAAA 
TCACTAGAGA TGTCAGAACG AATGAAGATT GTGGATGTGA TCGGGGAAAA GATCTATAAG 
GATGGAGAGC GAATAATCAC TCAGGGTGAA AAGGCCGACA GCTTTTATAT TATAGAGTCT 
GGAGAAGTGA GCATCTTGAT TAGAAGCAAG ACTAAAACGA ACAAGAACGG CGGGAACCAG 
GAGGTTGAGA TTGCCCACTG CCATAAGGGG CAGTACTTTG GAGAACTTGC CCTGGTGACC 
AACAAACCAA GAGCTGCTTC TGCTTATGCG GTTGGAGACG TCAAATGCTT AGTCATGGAT 
GTTCAAGCAT TTGAGAGGCT TCTGGGCCCC TGCATGGACA TCATGAAGAG GAACATCTCA 
CATTACGAAG AACAGCTGGT GAAGATGTTT GGCTCCAACT TGGATCTATT GGACCCCGGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062137.1
CDS	110..1315
Translation	MSHIQIPPGLTELLQGYTVEVLRQQPPDLVDFAVEYFTRLREARRQESDSFIAPPTTFHAQESSGVPVIEEDGESESDSDDEDLEVPIPSKFTRRVSVCAETFNPDEEEDNDPRVVHPKTDEQRYRLQEACKDILLFKNLDQEQLSQVLDAMFEKIVKTDEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSDGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLFKSLEMSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSKTKTNKNGGNQEVEIAHCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSNLDLLDPGQ

Target ORF information:

RefSeq Version	NM_019264.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase cAMP-dependent type II regulatory subunit alpha (Prkar2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019264.2

ORF Insert Sequence:

ATGAGCCACA TCCAGATCCC ACCGGGGCTC ACGGAGCTGC TGCAGGGCTA CACCGTGGAG 
GTGCTTCGGC AGCAGCCGCC CGACCTCGTC GACTTCGCGG TGGAGTACTT CACACGCCTG 
CGCGAGGCCC GCCGCCAGGA ATCAGACTCG TTCATCGCCC CCCCGACGAC CTTTCACGCG 
CAGGAGTCCA GCGGGGTCCC CGTCATCGAG GAGGACGGGG AGAGTGAATC GGACTCGGAC 
GATGAGGATC TGGAAGTTCC GATTCCCAGC AAATTTACTA GACGAGTATC AGTCTGTGCA 
GAAACGTTTA ACCCTGATGA AGAAGAAGAT AATGATCCAA GGGTGGTTCA CCCCAAAACC 
GACGAGCAGA GGTACAGACT TCAGGAAGCC TGTAAAGACA TTCTGCTTTT CAAAAACCTG 
GATCAGGAAC AGCTTTCTCA AGTTCTGGAC GCCATGTTTG AAAAGATAGT CAAAACTGAC 
GAGCATGTCA TTGACCAAGG AGATGATGGA GACAACTTTT ATGTCATAGA AAGGGGAACC 
TATGACATTT TAGTAACAAA AGATAATCAA ACACGATCTG TTGGTCAGTA TGACAACCGT 
GGCAGTTTTG GAGAACTAGC CCTGATGTAC AATACCCCGA GAGCTGCTAC CATTGTGGCC 
ACCTCAGACG GCTCCCTTTG GGGATTGGAC CGGGTGACTT TTAGGAGAAT CATAGTGAAG 
AACAATGCAA AGAAGAGGAA GATGTTCGAA TCGTTTATTG AGTCTGTACC GCTCTTTAAA 
TCACTAGAGA TGTCAGAACG AATGAAGATT GTGGATGTGA TCGGGGAAAA GATCTATAAG 
GATGGAGAGC GAATAATCAC TCAGGGTGAA AAGGCCGACA GCTTTTATAT TATAGAGTCT 
GGAGAAGTGA GCATCTTGAT TAGAAGCAAG ACTAAAACGA ACAAGAACGG CGGGAACCAG 
GAGGTTGAGA TTGCCCACTG CCATAAGGGG CAGTACTTTG GAGAACTTGC CCTGGTGACC 
AACAAACCAA GAGCTGCTTC TGCTTATGCG GTTGGAGACG TCAAATGCTT AGTCATGGAT 
GTTCAAGCAT TTGAGAGGCT TCTGGGCCCC TGCATGGACA TCATGAAGAG GAACATCTCA 
CATTACGAAG AACAGCTGGT GAAGATGTTT GGCTCCAACT TGGATCTATT GGACCCCGGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkar2a ( XM_006243713.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243775.1 Rattus norvegicus protein kinase cAMP-dependent type 2 regulatory subunit alpha (Prkar2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa31546
Clone ID Related Accession (Same CDS sequence)	XM_006243713.3
Accession Version	XM_006243713.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase type II-alpha regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGT GTCTTCAAAC CCTCTATGGT GGTGATACTT CAGGAAAAGA GGTTCAAGTA 
CTTTTCACTG GCTTTTATGG AGGAGGAAGA ACAAAAGTTC CGATTCCCAG CAAATTTACT 
AGACGAGTAT CAGTCTGTGC AGAAACGTTT AACCCTGATG AAGAAGAAGA TAATGATCCA 
AGGGTGGTTC ACCCCAAAAC CGACGAGCAG AGGTGCAGAC TTCAGGAAGC CTGTAAAGAC 
ATTCTGCTTT TCAAAAACCT GGATCAGGAA CAGCTTTCTC AAGTTCTGGA CGCCATGTTT 
GAAAAGATAG TCAAAACTGA CGAGCATGTC ATTGACCAAG GAGATGATGG AGACAACTTT 
TATGTCATAG AAAGGGGAAC CTATGACATT TTAGTAACAA AAGATAATCA AACACGATCT 
GTTGGTCAGT ATGACAACCG TGGCAGTTTT GGAGAACTAG CCCTGATGTA CAATACCCCG 
AGAGCTGCTA CCATTGTGGC CACCTCAGAC GGCTCCCTTT GGGGATTGGA CCGGGTGACT 
TTTAGGAGAA TCATAGTGAA GAACAATGCA AAGAAGAGGA AGATGTTCGA ATCGTTTATT 
GAGTCTGTAC CGCTCTTTAA ATCACTAGAG ATGTCAGAAC GAATGAAGAT TGTGGATGTG 
ATCGGGGAAA AGATCTATAA GGATGGAGAG CGAATAATCA CTCAGGGTGA AAAGGCCGAC 
AGCTTTTATA TTATAGAGTC TGGAGAAGTG AGCATCTTGA TTAGAAGCAA GACTAAAACG 
AACAAGAACG GCGGGAACCA GGAGGTTGAG ATTGCCCACT GCCATAAGGG GCAGTACTTT 
GGAGAACTTG CCCTGGTGAC CAACAAACCA AGAGCTGCTT CTGCTTATGC GGTTGGAGAT 
GTCAAATGCT TAGTCATGGA TGTTCAAGCA TTTGAGAGGC TTCTGGGCCC CTGCATGGAC 
ATCATGAAGA GGAACATCTC ACATTACGAA GAACAGCTGG TGAAGATGTT TGGCTCCAAC 
TTGGATCTGT TGGACCCCGG GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243775.1
CDS	259..1305
Translation	MSLCLQTLYGGDTSGKEVQVLFTGFYGGGRTKVPIPSKFTRRVSVCAETFNPDEEEDNDPRVVHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFEKIVKTDEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSDGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLFKSLEMSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSKTKTNKNGGNQEVEIAHCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSNLDLLDPGQ

Target ORF information:

RefSeq Version	XM_006243713.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase cAMP-dependent type 2 regulatory subunit alpha (Prkar2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243713.3

ORF Insert Sequence:

ATGAGCTTGT GTCTTCAAAC CCTCTATGGT GGTGATACTT CAGGAAAAGA GGTTCAAGTA 
CTTTTCACTG GCTTTTATGG AGGAGGAAGA ACAAAAGTTC CGATTCCCAG CAAATTTACT 
AGACGAGTAT CAGTCTGTGC AGAAACGTTT AACCCTGATG AAGAAGAAGA TAATGATCCA 
AGGGTGGTTC ACCCCAAAAC CGACGAGCAG AGGTGCAGAC TTCAGGAAGC CTGTAAAGAC 
ATTCTGCTTT TCAAAAACCT GGATCAGGAA CAGCTTTCTC AAGTTCTGGA CGCCATGTTT 
GAAAAGATAG TCAAAACTGA CGAGCATGTC ATTGACCAAG GAGATGATGG AGACAACTTT 
TATGTCATAG AAAGGGGAAC CTATGACATT TTAGTAACAA AAGATAATCA AACACGATCT 
GTTGGTCAGT ATGACAACCG TGGCAGTTTT GGAGAACTAG CCCTGATGTA CAATACCCCG 
AGAGCTGCTA CCATTGTGGC CACCTCAGAC GGCTCCCTTT GGGGATTGGA CCGGGTGACT 
TTTAGGAGAA TCATAGTGAA GAACAATGCA AAGAAGAGGA AGATGTTCGA ATCGTTTATT 
GAGTCTGTAC CGCTCTTTAA ATCACTAGAG ATGTCAGAAC GAATGAAGAT TGTGGATGTG 
ATCGGGGAAA AGATCTATAA GGATGGAGAG CGAATAATCA CTCAGGGTGA AAAGGCCGAC 
AGCTTTTATA TTATAGAGTC TGGAGAAGTG AGCATCTTGA TTAGAAGCAA GACTAAAACG 
AACAAGAACG GCGGGAACCA GGAGGTTGAG ATTGCCCACT GCCATAAGGG GCAGTACTTT 
GGAGAACTTG CCCTGGTGAC CAACAAACCA AGAGCTGCTT CTGCTTATGC GGTTGGAGAT 
GTCAAATGCT TAGTCATGGA TGTTCAAGCA TTTGAGAGGC TTCTGGGCCC CTGCATGGAC 
ATCATGAAGA GGAACATCTC ACATTACGAA GAACAGCTGG TGAAGATGTT TGGCTCCAAC 
TTGGATCTGT TGGACCCCGG GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A systematic evaluation of protein kinase A-A-kinase anchoring protein interaction motifs.
Biochemistry54(1)11-21(2015 Jan)
Burgers PP,van der Heyden MA,Kok B,Heck AJ,Scholten A

Cytoplasmic anchoring of cAMP-dependent protein kinase (PKA) by A-kinase anchor proteins (AKAPs) is required for meiotic arrest of porcine full-grown and growing oocytes.
Biology of reproduction90(3)58(2014 Mar)
Nishimura T,Fujii W,Sugiura K,Naito K

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
The EMBO journal33(2)157-70(2014 Jan)
Biesemann C,Gr?nborg M,Luquet E,Wichert SP,Bernard V,Bungers SR,Cooper B,Varoqueaux F,Li L,Byrne JA,Urlaub H,Jahn O,Brose N,Herzog E

A-kinase anchor protein 1 (AKAP1) regulates cAMP-dependent protein kinase (PKA) localization and is involved in meiotic maturation of porcine oocytes.
Biology of reproduction88(4)85(2013 Apr)
Nishimura T,Sugiura K,Naito K

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Prkar2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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