Gpam cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpam
Entrez Gene ID	29653
Full Name	glycerol-3-phosphate acyltransferase, mitochondrial
General protein information	Preferred Names glycerol-3-phosphate acyltransferase, mitochondrial Names glycerol-3-phosphate acyltransferase 1, mitochondrial GPAT GPAT-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q55
Summary	mitochondrial glycerol-phosphate acyltransferase enzyme; regulated by phosphorylation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008760534.1	XP_008758756.1	glycerol-3-phosphate acyltransferase 1, mitochondrial isoform X1
XM_006231626.3	XP_006231688.1	glycerol-3-phosphate acyltransferase 1, mitochondrial isoform X1
XM_006231625.3	XP_006231687.1	glycerol-3-phosphate acyltransferase 1, mitochondrial isoform X1
NM_017274.1	NP_058970.1	glycerol-3-phosphate acyltransferase 1, mitochondrial

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG5508	NP_733237.1
Mus musculus (house mouse)	Gpam	NP_032175.2
Gallus gallus (chicken)	GPAM	XP_421757.2
Homo sapiens (human)	GPAM	NP_001231878.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003687	XP_313470.4
	GPAM	XP_001088103.1
Bos taurus (cattle)	GPAM	NP_001012282.1
Rattus norvegicus (Norway rat)	Gpam	NP_058970.1
Xenopus tropicalis (tropical clawed frog)	MGC145183	NP_001072564.1
Pan troglodytes (chimpanzee)	GPAM	XP_508037.2
Canis lupus familiaris (dog)	GPAM	XP_005637781.1
Danio rerio (zebrafish)	gpam	XP_002664023.2
Caenorhabditis elegans (roundworm)	acl-6	NP_001023769.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptional regulation of mitochondrial glycerophosphate acyltransferase is mediated by distal promoter via ChREBP and SREBP-1.
Guha P, Aneja KK, Shilpi RY, Haldar D
Archives of biochemistry and biophysics490(2)85-95(2009 Oct)

Glycerol-3-phosphate acyltransferase 1 is essential for the immune response to infection with coxsackievirus B3 in mice.
Karlsson EA, Wang S, Shi Q, Coleman RA, Beck MA
The Journal of nutrition139(4)779-83(2009 Apr)

Atorvastatin prevents carbohydrate response element binding protein activation in the fructose-fed rat by activating protein kinase A.
Rodr?guez-Calvo R, Barroso E, Serrano L, Coll T, S?nchez RM, Merlos M, Palomer X, Laguna JC, V?zquez-Carrera M
Hepatology (Baltimore, Md.)49(1)106-15(2009 Jan)

Glycerol-3-phosphate acyltransferase-1 regulates murine T-lymphocyte proliferation and cytokine production.
Collison LW, Murphy EJ, Jolly CA
American journal of physiology. Cell physiology295(6)C1543-9(2008 Dec)

Expression profiling of hepatic genes associated with lipid metabolism in nephrotic rats.
Zhou Y, Zhang X, Chen L, Wu J, Dang H, Wei M, Fan Y, Zhang Y, Zhu Y, Wang N, Breyer MD, Guan Y
American journal of physiology. Renal physiology295(3)F662-71(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ChREBP and SREBP-1 bind to the mtGPAT distal promoter.
Title: Transcriptional regulation of mitochondrial glycerophosphate acyltransferase is mediated by distal promoter via ChREBP and SREBP-1.

Taken together, the results suggest that there are two promoters for rat mtGPAT gene and that the transcriptional regulation is mediated through the distal promoter.
Title: The presence of distal and proximal promoters for rat mitochondrial glycerol-3-phosphate acyltransferase.

These results demonstrate that two pools of GPAT1 are present in rat liver mitochondria: an active one, located in outer mitochondrial membrane and a less active one, located in membranes associated with both mitochondria and endoplasmic reticulum.
Title: Mitochondrial glycerol-3-P acyltransferase 1 is most active in outer mitochondrial membrane but not in mitochondrial associated vesicles (MAV).

TXow mitochondrial isoforms have been identified, one being sensitive in phosphorylation in vitro.
Title: Phosphorylation regulates mitochondrial glycerol-3-phosphate-1 acyltransferase activity in T-lymphocytes.

Casein kinase 2 phosphorylates liver mitochondrial glycerol-3-phosphate acyltransferase.
Title: Phosphorylation of rat liver mitochondrial glycerol-3-phosphate acyltransferase by casein kinase 2.

The following Gpam gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpam cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12429	NM_017274.1 Latest version!	Rattus norvegicus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$489.30 $699.00
ORa24238	XM_008760534.1 Latest version!	Rattus norvegicus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa24238	XM_006231626.3 Latest version!	Rattus norvegicus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa24238	XM_006231625.3 Latest version!	Rattus norvegicus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpam ( NM_017274.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058970.1 Rattus norvegicus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), mRNA.

Price & Availability↑

CloneID	ORa12429
Clone ID Related Accession (Same CDS sequence)	NM_017274.1
Accession Version	NM_017274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	glycerol-3-phosphate acyltransferase 1, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF021348.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF021348.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGAGGAGT CTTCAGTGAC AATTGGCACA ATAGACGTTT CTTATCTGCC CAATTCATCA 
GAATACAGCC TTGGCCGATG TAAACACACG AATGAGGACT GGGTTGACTG TGGCTTCAAA 
CCTACCTTCT TCAGATCCGC AACGCTGAAA TGGAAGGAGA GCCTCATGAG CCGGAAGAGG 
CCCTTCGTGG GAAGGTGTTG CTATTCATGC ACGCCTCAGA GCTGGGAAAG GTTTTTCAAC 
CCCAGTATCC CATCTCTGGG TTTGCGGAAT GTTATTTATA TCAATGAAAC TCACACAAGG 
CACCGAGGAT GGCTGGCAAG ACGGCTTTCT TACATCCTTT TTGTTCAAGA GCGCGATGTC 
CACAAGGGCA TGTTTGCCAC CAGTATCACT GACAATGTAC TGAATAGCAG CAGAGTCCAA 
GAGGCAATTG CTGAAGTGGC TGCAGAATTG AACCCGGATG GATCTGCCCA GCAGCAGTCC 
AAAGCCATCC AGAAAGTGAA AAGGAAAGCC AGGAAGATCC TCCAGGAAAT GGTTGCTACA 
GTCTCCCCCG GGATGATCAG GCTGACTGGC TGGGTGTTAC TAAAGCTCTT CAACAGCTTC 
TTCTGGAACA TTCAGATTCA CAAGGGTCAA CTTGAGATGG TGAAAGCTGC AACTGAGACG 
AATCTGCCGC TCTTGTTTCT GCCGGTGCAC AGATCCCACA TCGACTACCT GCTGCTCACC 
TTCATCCTCT TCTGCCACAA CATCAAAGCT CCATACATCG CCTCGGGCAA CAACCTCAAC 
ATCCCCATCT TCAGTACCTT GATTCACAAG CTTGGGGGCT TTTTCATAAG ACGGAGGCTT 
GACGAAACTC CAGATGGACG CAAAGACATT CTGTACAGAG CGTTGCTCCA TGGGCATATA 
GTTGAACTCC TCCGACAGCA GCAGTTCCTG GAGATCTTCC TGGAAGGCAC CCGCTCCCGC 
AGTGGCAAGA CCTCCTGTGC CCGGGCCGGG CTCCTGTCAG TGGTAGTGGA TACTCTGTCA 
TCCAACACCA TCCCTGACAT CCTGGTCATC CCTGTGGGCA TCTCGTATGA TCGGATAATC 
GAAGGTCACT ACAATGGTGA ACAGTTGGGC AAGCCCAAGA AGAATGAAAG TCTCTGGAGT 
GTGGCAAGAG GCGTTATCAG AATGCTGCGG AAAAACTACG GCTATGTCCG AGTGGACTTT 
GCACAGCCAT TTTCTTTGAA GGAATATTTA GAAGGCCAAA GTCAGAAACC TGTATCTGCT 
CCCCTCTCTT TGGAGCAAGC ACTGTTACCA GCAATCCTTC CTTCAAGACC TGATGCTGCT 
GCTGCCGAAC ATGAAGACAT GTCCAGTAAT GAGTCGAGAA ACGCGGCAGA CGAAGCCTTC 
CGAAGGAGGC TGATCGCAAA CCTGGCGGAG CACATTCTCT TCACCGCCAG CAAGTCCTGC 
GCTATCATGT CCACCCACAT TGTGGCCTGC CTGCTCCTCT ACAGACACAG GCAGGGAATC 
CACCTCTCCA CGCTGGTGGA AGACTTCTTT GTGATGAAGG AGGAAGTCCT AGCTCGGGAT 
TTTGACCTGG GCTTCTCCGG GAATTCAGAA GATGTAGTCA TGCATGCTAT TCAGCTTCTG 
GGGAACTGTG TCACAATCAC CCACACTAGC AGGAAGGATG AATTCTTTAT TACTCCCAGC 
ACAACTGTCC CGTCCGTCTT TGAACTCAAC TTCTACAGCA ATGGGGTACT TCATGTCTTT 
ATCATGGAAG CCATCATAGC TTGCAGCATT TATGCAGTCC AGAATAAGAG GGGTTCCGGA 
GGGTCTGCCG GAGGCCTTGG CAACCTGATC AGCCAGGAGC AGCTGGTGCG GAAGGCCGCC 
AGCCTGTGCT ACCTTCTCTC TAATGAAGGT ACCATTTCTC TGCCCTGCCA GACATTTTAC 
CAGGTTTGTC AAGAGACAGT AGGAAAGTTC ATCCAGTACG GAATTCTCAC AGTGGCAGAG 
CAAGATGACC AGGAAGATGT CAGTCCTGGC CTTGCAGAGC AGCAGTGGAA CAAGAAGCTT 
CCGGAGCCTC TGAACTGGAG AAGTGACGAA GAAGATGAGG ACAGTGACTT TGGTGAGGAG 
CAGCGTGATT GCTACCTGAA GGTGAGCCAG GCCAAGGAGC ACCAGCAATT CATCACCTTT 
CTGCAGAGGC TTCTGGGGCC CCTGCTAGAA GCCTACAGCT CTGCTGCCAT CTTTGTCCAC 
ACCTTCCGCG GCCCAGTCCC GGAGTCTGAG TACCTGCAGA AGCTGCACAG GTACCTTCTC 
ACCAGGACGG AGAGGAACGT CGCGGTGTAC GCTGAGAGTG CCACATACTG TCTTGTGAAG 
AATGCTGTGA AAATGTTTAA GGACATCGGG GTTTTCAAAG AGACCAAGCA GAAGCGAGCG 
TCTGTCTTAG AACTGAGCAC CACTTTCCTA CCTCAGGGCA GCCGGCAGAA GCTCCTGGAA 
TACATTCTGA GCTTCGTGGT GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058970.1
CDS	160..2646
Translation	MEESSVTIGTIDVSYLPNSSEYSLGRCKHTNEDWVDCGFKPTFFRSATLKWKESLMSRKRPFVGRCCYSCTPQSWERFFNPSIPSLGLRNVIYINETHTRHRGWLARRLSYILFVQERDVHKGMFATSITDNVLNSSRVQEAIAEVAAELNPDGSAQQQSKAIQKVKRKARKILQEMVATVSPGMIRLTGWVLLKLFNSFFWNIQIHKGQLEMVKAATETNLPLLFLPVHRSHIDYLLLTFILFCHNIKAPYIASGNNLNIPIFSTLIHKLGGFFIRRRLDETPDGRKDILYRALLHGHIVELLRQQQFLEIFLEGTRSRSGKTSCARAGLLSVVVDTLSSNTIPDILVIPVGISYDRIIEGHYNGEQLGKPKKNESLWSVARGVIRMLRKNYGYVRVDFAQPFSLKEYLEGQSQKPVSAPLSLEQALLPAILPSRPDAAAAEHEDMSSNESRNAADEAFRRRLIANLAEHILFTASKSCAIMSTHIVACLLLYRHRQGIHLSTLVEDFFVMKEEVLARDFDLGFSGNSEDVVMHAIQLLGNCVTITHTSRKDEFFITPSTTVPSVFELNFYSNGVLHVFIMEAIIACSIYAVQNKRGSGGSAGGLGNLISQEQLVRKAASLCYLLSNEGTISLPCQTFYQVCQETVGKFIQYGILTVAEQDDQEDVSPGLAEQQWNKKLPEPLNWRSDEEDEDSDFGEEQRDCYLKVSQAKEHQQFITFLQRLLGPLLEAYSSAAIFVHTFRGPVPESEYLQKLHRYLLTRTERNVAVYAESATYCLVKNAVKMFKDIGVFKETKQKRASVLELSTTFLPQGSRQKLLEYILSFVVL

Target ORF information:

RefSeq Version	NM_017274.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017274.1

ORF Insert Sequence:

ATGGAGGAGT CTTCAGTGAC AATTGGCACA ATAGACGTTT CTTATCTGCC CAATTCATCA 
GAATACAGCC TTGGCCGATG TAAACACACG AATGAGGACT GGGTTGACTG TGGCTTCAAA 
CCTACCTTCT TCAGATCCGC AACGCTGAAA TGGAAGGAGA GCCTCATGAG CCGGAAGAGG 
CCCTTCGTGG GAAGGTGTTG CTATTCATGC ACGCCTCAGA GCTGGGAAAG GTTTTTCAAC 
CCCAGTATCC CATCTCTGGG TTTGCGGAAT GTTATTTATA TCAATGAAAC TCACACAAGG 
CACCGAGGAT GGCTGGCAAG ACGGCTTTCT TACATCCTTT TTGTTCAAGA GCGCGATGTC 
CACAAGGGCA TGTTTGCCAC CAGTATCACT GACAATGTAC TGAATAGCAG CAGAGTCCAA 
GAGGCAATTG CTGAAGTGGC TGCAGAATTG AACCCGGATG GATCTGCCCA GCAGCAGTCC 
AAAGCCATCC AGAAAGTGAA AAGGAAAGCC AGGAAGATCC TCCAGGAAAT GGTTGCTACA 
GTCTCCCCCG GGATGATCAG GCTGACTGGC TGGGTGTTAC TAAAGCTCTT CAACAGCTTC 
TTCTGGAACA TTCAGATTCA CAAGGGTCAA CTTGAGATGG TGAAAGCTGC AACTGAGACG 
AATCTGCCGC TCTTGTTTCT GCCGGTGCAC AGATCCCACA TCGACTACCT GCTGCTCACC 
TTCATCCTCT TCTGCCACAA CATCAAAGCT CCATACATCG CCTCGGGCAA CAACCTCAAC 
ATCCCCATCT TCAGTACCTT GATTCACAAG CTTGGGGGCT TTTTCATAAG ACGGAGGCTT 
GACGAAACTC CAGATGGACG CAAAGACATT CTGTACAGAG CGTTGCTCCA TGGGCATATA 
GTTGAACTCC TCCGACAGCA GCAGTTCCTG GAGATCTTCC TGGAAGGCAC CCGCTCCCGC 
AGTGGCAAGA CCTCCTGTGC CCGGGCCGGG CTCCTGTCAG TGGTAGTGGA TACTCTGTCA 
TCCAACACCA TCCCTGACAT CCTGGTCATC CCTGTGGGCA TCTCGTATGA TCGGATAATC 
GAAGGTCACT ACAATGGTGA ACAGTTGGGC AAGCCCAAGA AGAATGAAAG TCTCTGGAGT 
GTGGCAAGAG GCGTTATCAG AATGCTGCGG AAAAACTACG GCTATGTCCG AGTGGACTTT 
GCACAGCCAT TTTCTTTGAA GGAATATTTA GAAGGCCAAA GTCAGAAACC TGTATCTGCT 
CCCCTCTCTT TGGAGCAAGC ACTGTTACCA GCAATCCTTC CTTCAAGACC TGATGCTGCT 
GCTGCCGAAC ATGAAGACAT GTCCAGTAAT GAGTCGAGAA ACGCGGCAGA CGAAGCCTTC 
CGAAGGAGGC TGATCGCAAA CCTGGCGGAG CACATTCTCT TCACCGCCAG CAAGTCCTGC 
GCTATCATGT CCACCCACAT TGTGGCCTGC CTGCTCCTCT ACAGACACAG GCAGGGAATC 
CACCTCTCCA CGCTGGTGGA AGACTTCTTT GTGATGAAGG AGGAAGTCCT AGCTCGGGAT 
TTTGACCTGG GCTTCTCCGG GAATTCAGAA GATGTAGTCA TGCATGCTAT TCAGCTTCTG 
GGGAACTGTG TCACAATCAC CCACACTAGC AGGAAGGATG AATTCTTTAT TACTCCCAGC 
ACAACTGTCC CGTCCGTCTT TGAACTCAAC TTCTACAGCA ATGGGGTACT TCATGTCTTT 
ATCATGGAAG CCATCATAGC TTGCAGCATT TATGCAGTCC AGAATAAGAG GGGTTCCGGA 
GGGTCTGCCG GAGGCCTTGG CAACCTGATC AGCCAGGAGC AGCTGGTGCG GAAGGCCGCC 
AGCCTGTGCT ACCTTCTCTC TAATGAAGGT ACCATTTCTC TGCCCTGCCA GACATTTTAC 
CAGGTTTGTC AAGAGACAGT AGGAAAGTTC ATCCAGTACG GAATTCTCAC AGTGGCAGAG 
CAAGATGACC AGGAAGATGT CAGTCCTGGC CTTGCAGAGC AGCAGTGGAA CAAGAAGCTT 
CCGGAGCCTC TGAACTGGAG AAGTGACGAA GAAGATGAGG ACAGTGACTT TGGTGAGGAG 
CAGCGTGATT GCTACCTGAA GGTGAGCCAG GCCAAGGAGC ACCAGCAATT CATCACCTTT 
CTGCAGAGGC TTCTGGGGCC CCTGCTAGAA GCCTACAGCT CTGCTGCCAT CTTTGTCCAC 
ACCTTCCGCG GCCCAGTCCC GGAGTCTGAG TACCTGCAGA AGCTGCACAG GTACCTTCTC 
ACCAGGACGG AGAGGAACGT CGCGGTGTAC GCTGAGAGTG CCACATACTG TCTTGTGAAG 
AATGCTGTGA AAATGTTTAA GGACATCGGG GTTTTCAAAG AGACCAAGCA GAAGCGAGCG 
TCTGTCTTAG AACTGAGCAC CACTTTCCTA CCTCAGGGCA GCCGGCAGAA GCTCCTGGAA 
TACATTCTGA GCTTCGTGGT GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpam ( XM_008760534.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758756.1 Rattus norvegicus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa24238
Clone ID Related Accession (Same CDS sequence)	XM_008760534.1 , XM_006231626.3 , XM_006231625.3
Accession Version	XM_008760534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glycerol-3-phosphate acyltransferase 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT CTTCAGTGAC AATTGGCACA ATAGACGTTT CTTATCTGCC CAATTCATCA 
GAATACAGCC TTGGCCGATG TAAACACACG AATGAGGACT GGGTTGACTG TGGCTTCAAA 
CCTACCTTCT TCAGATCCGC AACGCTGAAA TGGAAGGAGA GCCTCATGAG CCGGAAGAGG 
CCCTTCGTGG GAAGGTGTTG CTATTCATGC ACGCCTCAGA GCTGGGAAAG GTTTTTCAAC 
CCCAGTATCC CATCTCTGGG TTTGCGGAAT GTTATTTATA TCAATGAAAC TCACACAAGG 
CACCGAGGAT GGCTGGCAAG ACGGCTTTCT TACATCCTTT TTGTTCAAGA GCGCGATGTC 
CACAAGGGCA TGTTTGCCAC CAGTATCACT GACAATGTAC TGAATAGCAG CAGAGTCCAA 
GAGGCAATTG CTGAAGTGGC TGCAGAATTG AACCCGGATG GATCTGCCCA GCAGCAGTCC 
AAAGCCATCC AGAAAGTGAA AAGGAAAGCC AGGAAGATCC TTCAGGAAAT GGTTGCTACA 
GTCTCCCCCG GGATGATCAG GCTGACTGGC TGGGTGTTAC TAAAGCTCTT CAACAGCTTC 
TTCTGGAACA TTCAGATTCA CAAGGGTCAA CTTGAGATGG TGAAAGCTGC AACTGAGACG 
AATCTGCCGC TCTTGTTTCT GCCGGTGCAC AGATCCCACA TCGACTACCT GCTGCTCACC 
TTCATCCTCT TCTGCCACAA CATCAAAGCT CCATACATCG CCTCGGGCAA CAACCTCAAC 
ATCCCCATCT TCAGTACCTT GATTCACAAG CTTGGGGGCT TTTTCATAAG ACGGAGGCTT 
GACGAAACTC CAGATGGACG CAAAGACATT CTGTACAGAG CGTTGCTCCA TGGGCATATA 
GTTGAACTCC TCCGACAGCA GCAGTTCCTG GAGATCTTCC TGGAAGGCAC CCGCTCCCGC 
AGTGGCAAGA CCTCCTGTGC CCGGGCCGGG CTCCTGTCAG TGGTAGTGGA TACTCTGTCA 
TCCAACACCA TCCCTGACAT CCTGGTCATC CCTGTGGGCA TCTCGTATGA TCGGATAATC 
GAAGGTCACT ACAATGGTGA ACAGTTGGGC AAGCCCAAGA AGAATGAAAG TCTCTGGAGT 
GTGGCAAGAG GCGTTATCAG AATGCTGCGG AAAAACTACG GCTATGTCCG AGTGGACTTT 
GCACAGCCAT TTTCTTTGAA GGAATATTTA GAAGGCCAAA GTCAGAAACC TGTATCTGCT 
CCCCTCTCTT TGGAGCAAGC ACTGTTACCA GCAATCCTTC CTTCAAGACC TGATGCTGCT 
GCTGCCGAAC ATGAAGACAT GTCCAGTAAT GAGTCGAGAA ACGCGGCAGA CGAAGCCTTC 
CGAAGGAGGC TGATCACAAA CCTGGCGGAG CACATTCTCT TCACCGCCAG CAAGTCCTGC 
GCTATCATGT CCACCCACAT TGTGGCCTGC CTGCTCCTCT ACAGACACAG GCAGGGAATC 
CACCTCTCCA CGCTGGTGGA AGACTTCTTT GTGATGAAGG AGGAAGTCCT AGCTCGGGAT 
TTTGACCTGG GCTTCTCCGG GAATTCAGAA GACGTAGTCA TGCATGCTAT TCAGCTTCTG 
GGGAACTGTG TCACAATCAC CCACACTAGC AGGAAGGATG AATTCTTTAT TACTCCCAGC 
ACAACTGTCC CGTCCGTCTT TGAACTCAAC TTCTACAGCA ATGGGGTACT TCATGTCTTT 
ATCATGGAAG CCATCATAGC TTGCAGCATT TATGCAGTCC AGAATAAGAG GGGTTCCGGA 
GGGTCTGCCG GAGGCCTTGG CAACCTGATC AGCCAGGAGC AGCTGGTGCG GAAGGCCGCC 
AGTCTGTGCT ACCTTCTCTC TAATGAAGGT ACCATTTCTC TGCCCTGCCA GACATTTTAC 
CAGGTTTGTC ACGAGACAGT AGGAAAGTTC ATCCAGTACG GAATTCTCAC AGTGGCAGAG 
CAAGATGACC AGGAAGATGT CAGTCCTGGC CTTGCAGAGC AGCAGTGGAA CAAGAAGCTT 
CCGGAGCCTC TGAACTGGAG AAGTGACGAA GAAGATGAGG ACAGTGACTT TGGTGAGGAG 
CAGCGTGATT GCTACCTGAA GGTGAGCCAG GCCAAGGAGC ACCAGCAATT CATCACCTTT 
CTGCAGAGGC TTCTGGGGCC CCTGCTAGAA GCCTACAGCT CTGCTGCCAT CTTTGTCCAC 
ACCTTCCGCG GCGCAGTCCC GGAGTCTGAG TACCTGCAGA AGCTGCACAG GTACCTTCTC 
ACCAGGACGG AGAGGAACGT CGCGGTGTAC GCTGAGAGTG CCACATACTG TCTTGTGAAG 
AATGCTGTGA AAATGTTTAA GGACATCGGG GTTTTCAAAG AGACCAAGCA GAAGCGAGCG 
TCTGTCTTAG AACTGAGCAC CACTTTCCTA CCTCAGGGCA GCCGGCAGAA GCTCCTGGAA 
TACATTCTGA GCTTCGTGGT GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758756.1
CDS	102..2588
Translation	MEESSVTIGTIDVSYLPNSSEYSLGRCKHTNEDWVDCGFKPTFFRSATLKWKESLMSRKRPFVGRCCYSCTPQSWERFFNPSIPSLGLRNVIYINETHTRHRGWLARRLSYILFVQERDVHKGMFATSITDNVLNSSRVQEAIAEVAAELNPDGSAQQQSKAIQKVKRKARKILQEMVATVSPGMIRLTGWVLLKLFNSFFWNIQIHKGQLEMVKAATETNLPLLFLPVHRSHIDYLLLTFILFCHNIKAPYIASGNNLNIPIFSTLIHKLGGFFIRRRLDETPDGRKDILYRALLHGHIVELLRQQQFLEIFLEGTRSRSGKTSCARAGLLSVVVDTLSSNTIPDILVIPVGISYDRIIEGHYNGEQLGKPKKNESLWSVARGVIRMLRKNYGYVRVDFAQPFSLKEYLEGQSQKPVSAPLSLEQALLPAILPSRPDAAAAEHEDMSSNESRNAADEAFRRRLITNLAEHILFTASKSCAIMSTHIVACLLLYRHRQGIHLSTLVEDFFVMKEEVLARDFDLGFSGNSEDVVMHAIQLLGNCVTITHTSRKDEFFITPSTTVPSVFELNFYSNGVLHVFIMEAIIACSIYAVQNKRGSGGSAGGLGNLISQEQLVRKAASLCYLLSNEGTISLPCQTFYQVCHETVGKFIQYGILTVAEQDDQEDVSPGLAEQQWNKKLPEPLNWRSDEEDEDSDFGEEQRDCYLKVSQAKEHQQFITFLQRLLGPLLEAYSSAAIFVHTFRGAVPESEYLQKLHRYLLTRTERNVAVYAESATYCLVKNAVKMFKDIGVFKETKQKRASVLELSTTFLPQGSRQKLLEYILSFVVL

Target ORF information:

RefSeq Version	XM_008760534.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760534.1

ORF Insert Sequence:

ATGGAGGAGT CTTCAGTGAC AATTGGCACA ATAGACGTTT CTTATCTGCC CAATTCATCA 
GAATACAGCC TTGGCCGATG TAAACACACG AATGAGGACT GGGTTGACTG TGGCTTCAAA 
CCTACCTTCT TCAGATCCGC AACGCTGAAA TGGAAGGAGA GCCTCATGAG CCGGAAGAGG 
CCCTTCGTGG GAAGGTGTTG CTATTCATGC ACGCCTCAGA GCTGGGAAAG GTTTTTCAAC 
CCCAGTATCC CATCTCTGGG TTTGCGGAAT GTTATTTATA TCAATGAAAC TCACACAAGG 
CACCGAGGAT GGCTGGCAAG ACGGCTTTCT TACATCCTTT TTGTTCAAGA GCGCGATGTC 
CACAAGGGCA TGTTTGCCAC CAGTATCACT GACAATGTAC TGAATAGCAG CAGAGTCCAA 
GAGGCAATTG CTGAAGTGGC TGCAGAATTG AACCCGGATG GATCTGCCCA GCAGCAGTCC 
AAAGCCATCC AGAAAGTGAA AAGGAAAGCC AGGAAGATCC TTCAGGAAAT GGTTGCTACA 
GTCTCCCCCG GGATGATCAG GCTGACTGGC TGGGTGTTAC TAAAGCTCTT CAACAGCTTC 
TTCTGGAACA TTCAGATTCA CAAGGGTCAA CTTGAGATGG TGAAAGCTGC AACTGAGACG 
AATCTGCCGC TCTTGTTTCT GCCGGTGCAC AGATCCCACA TCGACTACCT GCTGCTCACC 
TTCATCCTCT TCTGCCACAA CATCAAAGCT CCATACATCG CCTCGGGCAA CAACCTCAAC 
ATCCCCATCT TCAGTACCTT GATTCACAAG CTTGGGGGCT TTTTCATAAG ACGGAGGCTT 
GACGAAACTC CAGATGGACG CAAAGACATT CTGTACAGAG CGTTGCTCCA TGGGCATATA 
GTTGAACTCC TCCGACAGCA GCAGTTCCTG GAGATCTTCC TGGAAGGCAC CCGCTCCCGC 
AGTGGCAAGA CCTCCTGTGC CCGGGCCGGG CTCCTGTCAG TGGTAGTGGA TACTCTGTCA 
TCCAACACCA TCCCTGACAT CCTGGTCATC CCTGTGGGCA TCTCGTATGA TCGGATAATC 
GAAGGTCACT ACAATGGTGA ACAGTTGGGC AAGCCCAAGA AGAATGAAAG TCTCTGGAGT 
GTGGCAAGAG GCGTTATCAG AATGCTGCGG AAAAACTACG GCTATGTCCG AGTGGACTTT 
GCACAGCCAT TTTCTTTGAA GGAATATTTA GAAGGCCAAA GTCAGAAACC TGTATCTGCT 
CCCCTCTCTT TGGAGCAAGC ACTGTTACCA GCAATCCTTC CTTCAAGACC TGATGCTGCT 
GCTGCCGAAC ATGAAGACAT GTCCAGTAAT GAGTCGAGAA ACGCGGCAGA CGAAGCCTTC 
CGAAGGAGGC TGATCACAAA CCTGGCGGAG CACATTCTCT TCACCGCCAG CAAGTCCTGC 
GCTATCATGT CCACCCACAT TGTGGCCTGC CTGCTCCTCT ACAGACACAG GCAGGGAATC 
CACCTCTCCA CGCTGGTGGA AGACTTCTTT GTGATGAAGG AGGAAGTCCT AGCTCGGGAT 
TTTGACCTGG GCTTCTCCGG GAATTCAGAA GACGTAGTCA TGCATGCTAT TCAGCTTCTG 
GGGAACTGTG TCACAATCAC CCACACTAGC AGGAAGGATG AATTCTTTAT TACTCCCAGC 
ACAACTGTCC CGTCCGTCTT TGAACTCAAC TTCTACAGCA ATGGGGTACT TCATGTCTTT 
ATCATGGAAG CCATCATAGC TTGCAGCATT TATGCAGTCC AGAATAAGAG GGGTTCCGGA 
GGGTCTGCCG GAGGCCTTGG CAACCTGATC AGCCAGGAGC AGCTGGTGCG GAAGGCCGCC 
AGTCTGTGCT ACCTTCTCTC TAATGAAGGT ACCATTTCTC TGCCCTGCCA GACATTTTAC 
CAGGTTTGTC ACGAGACAGT AGGAAAGTTC ATCCAGTACG GAATTCTCAC AGTGGCAGAG 
CAAGATGACC AGGAAGATGT CAGTCCTGGC CTTGCAGAGC AGCAGTGGAA CAAGAAGCTT 
CCGGAGCCTC TGAACTGGAG AAGTGACGAA GAAGATGAGG ACAGTGACTT TGGTGAGGAG 
CAGCGTGATT GCTACCTGAA GGTGAGCCAG GCCAAGGAGC ACCAGCAATT CATCACCTTT 
CTGCAGAGGC TTCTGGGGCC CCTGCTAGAA GCCTACAGCT CTGCTGCCAT CTTTGTCCAC 
ACCTTCCGCG GCGCAGTCCC GGAGTCTGAG TACCTGCAGA AGCTGCACAG GTACCTTCTC 
ACCAGGACGG AGAGGAACGT CGCGGTGTAC GCTGAGAGTG CCACATACTG TCTTGTGAAG 
AATGCTGTGA AAATGTTTAA GGACATCGGG GTTTTCAAAG AGACCAAGCA GAAGCGAGCG 
TCTGTCTTAG AACTGAGCAC CACTTTCCTA CCTCAGGGCA GCCGGCAGAA GCTCCTGGAA 
TACATTCTGA GCTTCGTGGT GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpam ( XM_006231626.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231688.1 Rattus norvegicus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24238
Clone ID Related Accession (Same CDS sequence)	XM_008760534.1 , XM_006231626.3 , XM_006231625.3
Accession Version	XM_006231626.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glycerol-3-phosphate acyltransferase 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231626.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT CTTCAGTGAC AATTGGCACA ATAGACGTTT CTTATCTGCC CAATTCATCA 
GAATACAGCC TTGGCCGATG TAAACACACG AATGAGGACT GGGTTGACTG TGGCTTCAAA 
CCTACCTTCT TCAGATCCGC AACGCTGAAA TGGAAGGAGA GCCTCATGAG CCGGAAGAGG 
CCCTTCGTGG GAAGGTGTTG CTATTCATGC ACGCCTCAGA GCTGGGAAAG GTTTTTCAAC 
CCCAGTATCC CATCTCTGGG TTTGCGGAAT GTTATTTATA TCAATGAAAC TCACACAAGG 
CACCGAGGAT GGCTGGCAAG ACGGCTTTCT TACATCCTTT TTGTTCAAGA GCGCGATGTC 
CACAAGGGCA TGTTTGCCAC CAGTATCACT GACAATGTAC TGAATAGCAG CAGAGTCCAA 
GAGGCAATTG CTGAAGTGGC TGCAGAATTG AACCCGGATG GATCTGCCCA GCAGCAGTCC 
AAAGCCATCC AGAAAGTGAA AAGGAAAGCC AGGAAGATCC TTCAGGAAAT GGTTGCTACA 
GTCTCCCCCG GGATGATCAG GCTGACTGGC TGGGTGTTAC TAAAGCTCTT CAACAGCTTC 
TTCTGGAACA TTCAGATTCA CAAGGGTCAA CTTGAGATGG TGAAAGCTGC AACTGAGACG 
AATCTGCCGC TCTTGTTTCT GCCGGTGCAC AGATCCCACA TCGACTACCT GCTGCTCACC 
TTCATCCTCT TCTGCCACAA CATCAAAGCT CCATACATCG CCTCGGGCAA CAACCTCAAC 
ATCCCCATCT TCAGTACCTT GATTCACAAG CTTGGGGGCT TTTTCATAAG ACGGAGGCTT 
GACGAAACTC CAGATGGACG CAAAGACATT CTGTACAGAG CGTTGCTCCA TGGGCATATA 
GTTGAACTCC TCCGACAGCA GCAGTTCCTG GAGATCTTCC TGGAAGGCAC CCGCTCCCGC 
AGTGGCAAGA CCTCCTGTGC CCGGGCCGGG CTCCTGTCAG TGGTAGTGGA TACTCTGTCA 
TCCAACACCA TCCCTGACAT CCTGGTCATC CCTGTGGGCA TCTCGTATGA TCGGATAATC 
GAAGGTCACT ACAATGGTGA ACAGTTGGGC AAGCCCAAGA AGAATGAAAG TCTCTGGAGT 
GTGGCAAGAG GCGTTATCAG AATGCTGCGG AAAAACTACG GCTATGTCCG AGTGGACTTT 
GCACAGCCAT TTTCTTTGAA GGAATATTTA GAAGGCCAAA GTCAGAAACC TGTATCTGCT 
CCCCTCTCTT TGGAGCAAGC ACTGTTACCA GCAATCCTTC CTTCAAGACC TGATGCTGCT 
GCTGCCGAAC ATGAAGACAT GTCCAGTAAT GAGTCGAGAA ACGCGGCAGA CGAAGCCTTC 
CGAAGGAGGC TGATCACAAA CCTGGCGGAG CACATTCTCT TCACCGCCAG CAAGTCCTGC 
GCTATCATGT CCACCCACAT TGTGGCCTGC CTGCTCCTCT ACAGACACAG GCAGGGAATC 
CACCTCTCCA CGCTGGTGGA AGACTTCTTT GTGATGAAGG AGGAAGTCCT AGCTCGGGAT 
TTTGACCTGG GCTTCTCCGG GAATTCAGAA GACGTAGTCA TGCATGCTAT TCAGCTTCTG 
GGGAACTGTG TCACAATCAC CCACACTAGC AGGAAGGATG AATTCTTTAT TACTCCCAGC 
ACAACTGTCC CGTCCGTCTT TGAACTCAAC TTCTACAGCA ATGGGGTACT TCATGTCTTT 
ATCATGGAAG CCATCATAGC TTGCAGCATT TATGCAGTCC AGAATAAGAG GGGTTCCGGA 
GGGTCTGCCG GAGGCCTTGG CAACCTGATC AGCCAGGAGC AGCTGGTGCG GAAGGCCGCC 
AGTCTGTGCT ACCTTCTCTC TAATGAAGGT ACCATTTCTC TGCCCTGCCA GACATTTTAC 
CAGGTTTGTC ACGAGACAGT AGGAAAGTTC ATCCAGTACG GAATTCTCAC AGTGGCAGAG 
CAAGATGACC AGGAAGATGT CAGTCCTGGC CTTGCAGAGC AGCAGTGGAA CAAGAAGCTT 
CCGGAGCCTC TGAACTGGAG AAGTGACGAA GAAGATGAGG ACAGTGACTT TGGTGAGGAG 
CAGCGTGATT GCTACCTGAA GGTGAGCCAG GCCAAGGAGC ACCAGCAATT CATCACCTTT 
CTGCAGAGGC TTCTGGGGCC CCTGCTAGAA GCCTACAGCT CTGCTGCCAT CTTTGTCCAC 
ACCTTCCGCG GCGCAGTCCC GGAGTCTGAG TACCTGCAGA AGCTGCACAG GTACCTTCTC 
ACCAGGACGG AGAGGAACGT CGCGGTGTAC GCTGAGAGTG CCACATACTG TCTTGTGAAG 
AATGCTGTGA AAATGTTTAA GGACATCGGG GTTTTCAAAG AGACCAAGCA GAAGCGAGCG 
TCTGTCTTAG AACTGAGCAC CACTTTCCTA CCTCAGGGCA GCCGGCAGAA GCTCCTGGAA 
TACATTCTGA GCTTCGTGGT GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231688.1
CDS	516..3002
Translation	MEESSVTIGTIDVSYLPNSSEYSLGRCKHTNEDWVDCGFKPTFFRSATLKWKESLMSRKRPFVGRCCYSCTPQSWERFFNPSIPSLGLRNVIYINETHTRHRGWLARRLSYILFVQERDVHKGMFATSITDNVLNSSRVQEAIAEVAAELNPDGSAQQQSKAIQKVKRKARKILQEMVATVSPGMIRLTGWVLLKLFNSFFWNIQIHKGQLEMVKAATETNLPLLFLPVHRSHIDYLLLTFILFCHNIKAPYIASGNNLNIPIFSTLIHKLGGFFIRRRLDETPDGRKDILYRALLHGHIVELLRQQQFLEIFLEGTRSRSGKTSCARAGLLSVVVDTLSSNTIPDILVIPVGISYDRIIEGHYNGEQLGKPKKNESLWSVARGVIRMLRKNYGYVRVDFAQPFSLKEYLEGQSQKPVSAPLSLEQALLPAILPSRPDAAAAEHEDMSSNESRNAADEAFRRRLITNLAEHILFTASKSCAIMSTHIVACLLLYRHRQGIHLSTLVEDFFVMKEEVLARDFDLGFSGNSEDVVMHAIQLLGNCVTITHTSRKDEFFITPSTTVPSVFELNFYSNGVLHVFIMEAIIACSIYAVQNKRGSGGSAGGLGNLISQEQLVRKAASLCYLLSNEGTISLPCQTFYQVCHETVGKFIQYGILTVAEQDDQEDVSPGLAEQQWNKKLPEPLNWRSDEEDEDSDFGEEQRDCYLKVSQAKEHQQFITFLQRLLGPLLEAYSSAAIFVHTFRGAVPESEYLQKLHRYLLTRTERNVAVYAESATYCLVKNAVKMFKDIGVFKETKQKRASVLELSTTFLPQGSRQKLLEYILSFVVL

Target ORF information:

RefSeq Version	XM_006231626.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231626.3

ORF Insert Sequence:

ATGGAGGAGT CTTCAGTGAC AATTGGCACA ATAGACGTTT CTTATCTGCC CAATTCATCA 
GAATACAGCC TTGGCCGATG TAAACACACG AATGAGGACT GGGTTGACTG TGGCTTCAAA 
CCTACCTTCT TCAGATCCGC AACGCTGAAA TGGAAGGAGA GCCTCATGAG CCGGAAGAGG 
CCCTTCGTGG GAAGGTGTTG CTATTCATGC ACGCCTCAGA GCTGGGAAAG GTTTTTCAAC 
CCCAGTATCC CATCTCTGGG TTTGCGGAAT GTTATTTATA TCAATGAAAC TCACACAAGG 
CACCGAGGAT GGCTGGCAAG ACGGCTTTCT TACATCCTTT TTGTTCAAGA GCGCGATGTC 
CACAAGGGCA TGTTTGCCAC CAGTATCACT GACAATGTAC TGAATAGCAG CAGAGTCCAA 
GAGGCAATTG CTGAAGTGGC TGCAGAATTG AACCCGGATG GATCTGCCCA GCAGCAGTCC 
AAAGCCATCC AGAAAGTGAA AAGGAAAGCC AGGAAGATCC TTCAGGAAAT GGTTGCTACA 
GTCTCCCCCG GGATGATCAG GCTGACTGGC TGGGTGTTAC TAAAGCTCTT CAACAGCTTC 
TTCTGGAACA TTCAGATTCA CAAGGGTCAA CTTGAGATGG TGAAAGCTGC AACTGAGACG 
AATCTGCCGC TCTTGTTTCT GCCGGTGCAC AGATCCCACA TCGACTACCT GCTGCTCACC 
TTCATCCTCT TCTGCCACAA CATCAAAGCT CCATACATCG CCTCGGGCAA CAACCTCAAC 
ATCCCCATCT TCAGTACCTT GATTCACAAG CTTGGGGGCT TTTTCATAAG ACGGAGGCTT 
GACGAAACTC CAGATGGACG CAAAGACATT CTGTACAGAG CGTTGCTCCA TGGGCATATA 
GTTGAACTCC TCCGACAGCA GCAGTTCCTG GAGATCTTCC TGGAAGGCAC CCGCTCCCGC 
AGTGGCAAGA CCTCCTGTGC CCGGGCCGGG CTCCTGTCAG TGGTAGTGGA TACTCTGTCA 
TCCAACACCA TCCCTGACAT CCTGGTCATC CCTGTGGGCA TCTCGTATGA TCGGATAATC 
GAAGGTCACT ACAATGGTGA ACAGTTGGGC AAGCCCAAGA AGAATGAAAG TCTCTGGAGT 
GTGGCAAGAG GCGTTATCAG AATGCTGCGG AAAAACTACG GCTATGTCCG AGTGGACTTT 
GCACAGCCAT TTTCTTTGAA GGAATATTTA GAAGGCCAAA GTCAGAAACC TGTATCTGCT 
CCCCTCTCTT TGGAGCAAGC ACTGTTACCA GCAATCCTTC CTTCAAGACC TGATGCTGCT 
GCTGCCGAAC ATGAAGACAT GTCCAGTAAT GAGTCGAGAA ACGCGGCAGA CGAAGCCTTC 
CGAAGGAGGC TGATCACAAA CCTGGCGGAG CACATTCTCT TCACCGCCAG CAAGTCCTGC 
GCTATCATGT CCACCCACAT TGTGGCCTGC CTGCTCCTCT ACAGACACAG GCAGGGAATC 
CACCTCTCCA CGCTGGTGGA AGACTTCTTT GTGATGAAGG AGGAAGTCCT AGCTCGGGAT 
TTTGACCTGG GCTTCTCCGG GAATTCAGAA GACGTAGTCA TGCATGCTAT TCAGCTTCTG 
GGGAACTGTG TCACAATCAC CCACACTAGC AGGAAGGATG AATTCTTTAT TACTCCCAGC 
ACAACTGTCC CGTCCGTCTT TGAACTCAAC TTCTACAGCA ATGGGGTACT TCATGTCTTT 
ATCATGGAAG CCATCATAGC TTGCAGCATT TATGCAGTCC AGAATAAGAG GGGTTCCGGA 
GGGTCTGCCG GAGGCCTTGG CAACCTGATC AGCCAGGAGC AGCTGGTGCG GAAGGCCGCC 
AGTCTGTGCT ACCTTCTCTC TAATGAAGGT ACCATTTCTC TGCCCTGCCA GACATTTTAC 
CAGGTTTGTC ACGAGACAGT AGGAAAGTTC ATCCAGTACG GAATTCTCAC AGTGGCAGAG 
CAAGATGACC AGGAAGATGT CAGTCCTGGC CTTGCAGAGC AGCAGTGGAA CAAGAAGCTT 
CCGGAGCCTC TGAACTGGAG AAGTGACGAA GAAGATGAGG ACAGTGACTT TGGTGAGGAG 
CAGCGTGATT GCTACCTGAA GGTGAGCCAG GCCAAGGAGC ACCAGCAATT CATCACCTTT 
CTGCAGAGGC TTCTGGGGCC CCTGCTAGAA GCCTACAGCT CTGCTGCCAT CTTTGTCCAC 
ACCTTCCGCG GCGCAGTCCC GGAGTCTGAG TACCTGCAGA AGCTGCACAG GTACCTTCTC 
ACCAGGACGG AGAGGAACGT CGCGGTGTAC GCTGAGAGTG CCACATACTG TCTTGTGAAG 
AATGCTGTGA AAATGTTTAA GGACATCGGG GTTTTCAAAG AGACCAAGCA GAAGCGAGCG 
TCTGTCTTAG AACTGAGCAC CACTTTCCTA CCTCAGGGCA GCCGGCAGAA GCTCCTGGAA 
TACATTCTGA GCTTCGTGGT GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpam ( XM_006231625.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231687.1 Rattus norvegicus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa24238
Clone ID Related Accession (Same CDS sequence)	XM_008760534.1 , XM_006231626.3 , XM_006231625.3
Accession Version	XM_006231625.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glycerol-3-phosphate acyltransferase 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231625.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT CTTCAGTGAC AATTGGCACA ATAGACGTTT CTTATCTGCC CAATTCATCA 
GAATACAGCC TTGGCCGATG TAAACACACG AATGAGGACT GGGTTGACTG TGGCTTCAAA 
CCTACCTTCT TCAGATCCGC AACGCTGAAA TGGAAGGAGA GCCTCATGAG CCGGAAGAGG 
CCCTTCGTGG GAAGGTGTTG CTATTCATGC ACGCCTCAGA GCTGGGAAAG GTTTTTCAAC 
CCCAGTATCC CATCTCTGGG TTTGCGGAAT GTTATTTATA TCAATGAAAC TCACACAAGG 
CACCGAGGAT GGCTGGCAAG ACGGCTTTCT TACATCCTTT TTGTTCAAGA GCGCGATGTC 
CACAAGGGCA TGTTTGCCAC CAGTATCACT GACAATGTAC TGAATAGCAG CAGAGTCCAA 
GAGGCAATTG CTGAAGTGGC TGCAGAATTG AACCCGGATG GATCTGCCCA GCAGCAGTCC 
AAAGCCATCC AGAAAGTGAA AAGGAAAGCC AGGAAGATCC TTCAGGAAAT GGTTGCTACA 
GTCTCCCCCG GGATGATCAG GCTGACTGGC TGGGTGTTAC TAAAGCTCTT CAACAGCTTC 
TTCTGGAACA TTCAGATTCA CAAGGGTCAA CTTGAGATGG TGAAAGCTGC AACTGAGACG 
AATCTGCCGC TCTTGTTTCT GCCGGTGCAC AGATCCCACA TCGACTACCT GCTGCTCACC 
TTCATCCTCT TCTGCCACAA CATCAAAGCT CCATACATCG CCTCGGGCAA CAACCTCAAC 
ATCCCCATCT TCAGTACCTT GATTCACAAG CTTGGGGGCT TTTTCATAAG ACGGAGGCTT 
GACGAAACTC CAGATGGACG CAAAGACATT CTGTACAGAG CGTTGCTCCA TGGGCATATA 
GTTGAACTCC TCCGACAGCA GCAGTTCCTG GAGATCTTCC TGGAAGGCAC CCGCTCCCGC 
AGTGGCAAGA CCTCCTGTGC CCGGGCCGGG CTCCTGTCAG TGGTAGTGGA TACTCTGTCA 
TCCAACACCA TCCCTGACAT CCTGGTCATC CCTGTGGGCA TCTCGTATGA TCGGATAATC 
GAAGGTCACT ACAATGGTGA ACAGTTGGGC AAGCCCAAGA AGAATGAAAG TCTCTGGAGT 
GTGGCAAGAG GCGTTATCAG AATGCTGCGG AAAAACTACG GCTATGTCCG AGTGGACTTT 
GCACAGCCAT TTTCTTTGAA GGAATATTTA GAAGGCCAAA GTCAGAAACC TGTATCTGCT 
CCCCTCTCTT TGGAGCAAGC ACTGTTACCA GCAATCCTTC CTTCAAGACC TGATGCTGCT 
GCTGCCGAAC ATGAAGACAT GTCCAGTAAT GAGTCGAGAA ACGCGGCAGA CGAAGCCTTC 
CGAAGGAGGC TGATCACAAA CCTGGCGGAG CACATTCTCT TCACCGCCAG CAAGTCCTGC 
GCTATCATGT CCACCCACAT TGTGGCCTGC CTGCTCCTCT ACAGACACAG GCAGGGAATC 
CACCTCTCCA CGCTGGTGGA AGACTTCTTT GTGATGAAGG AGGAAGTCCT AGCTCGGGAT 
TTTGACCTGG GCTTCTCCGG GAATTCAGAA GACGTAGTCA TGCATGCTAT TCAGCTTCTG 
GGGAACTGTG TCACAATCAC CCACACTAGC AGGAAGGATG AATTCTTTAT TACTCCCAGC 
ACAACTGTCC CGTCCGTCTT TGAACTCAAC TTCTACAGCA ATGGGGTACT TCATGTCTTT 
ATCATGGAAG CCATCATAGC TTGCAGCATT TATGCAGTCC AGAATAAGAG GGGTTCCGGA 
GGGTCTGCCG GAGGCCTTGG CAACCTGATC AGCCAGGAGC AGCTGGTGCG GAAGGCCGCC 
AGTCTGTGCT ACCTTCTCTC TAATGAAGGT ACCATTTCTC TGCCCTGCCA GACATTTTAC 
CAGGTTTGTC ACGAGACAGT AGGAAAGTTC ATCCAGTACG GAATTCTCAC AGTGGCAGAG 
CAAGATGACC AGGAAGATGT CAGTCCTGGC CTTGCAGAGC AGCAGTGGAA CAAGAAGCTT 
CCGGAGCCTC TGAACTGGAG AAGTGACGAA GAAGATGAGG ACAGTGACTT TGGTGAGGAG 
CAGCGTGATT GCTACCTGAA GGTGAGCCAG GCCAAGGAGC ACCAGCAATT CATCACCTTT 
CTGCAGAGGC TTCTGGGGCC CCTGCTAGAA GCCTACAGCT CTGCTGCCAT CTTTGTCCAC 
ACCTTCCGCG GCGCAGTCCC GGAGTCTGAG TACCTGCAGA AGCTGCACAG GTACCTTCTC 
ACCAGGACGG AGAGGAACGT CGCGGTGTAC GCTGAGAGTG CCACATACTG TCTTGTGAAG 
AATGCTGTGA AAATGTTTAA GGACATCGGG GTTTTCAAAG AGACCAAGCA GAAGCGAGCG 
TCTGTCTTAG AACTGAGCAC CACTTTCCTA CCTCAGGGCA GCCGGCAGAA GCTCCTGGAA 
TACATTCTGA GCTTCGTGGT GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231687.1
CDS	547..3033
Translation	MEESSVTIGTIDVSYLPNSSEYSLGRCKHTNEDWVDCGFKPTFFRSATLKWKESLMSRKRPFVGRCCYSCTPQSWERFFNPSIPSLGLRNVIYINETHTRHRGWLARRLSYILFVQERDVHKGMFATSITDNVLNSSRVQEAIAEVAAELNPDGSAQQQSKAIQKVKRKARKILQEMVATVSPGMIRLTGWVLLKLFNSFFWNIQIHKGQLEMVKAATETNLPLLFLPVHRSHIDYLLLTFILFCHNIKAPYIASGNNLNIPIFSTLIHKLGGFFIRRRLDETPDGRKDILYRALLHGHIVELLRQQQFLEIFLEGTRSRSGKTSCARAGLLSVVVDTLSSNTIPDILVIPVGISYDRIIEGHYNGEQLGKPKKNESLWSVARGVIRMLRKNYGYVRVDFAQPFSLKEYLEGQSQKPVSAPLSLEQALLPAILPSRPDAAAAEHEDMSSNESRNAADEAFRRRLITNLAEHILFTASKSCAIMSTHIVACLLLYRHRQGIHLSTLVEDFFVMKEEVLARDFDLGFSGNSEDVVMHAIQLLGNCVTITHTSRKDEFFITPSTTVPSVFELNFYSNGVLHVFIMEAIIACSIYAVQNKRGSGGSAGGLGNLISQEQLVRKAASLCYLLSNEGTISLPCQTFYQVCHETVGKFIQYGILTVAEQDDQEDVSPGLAEQQWNKKLPEPLNWRSDEEDEDSDFGEEQRDCYLKVSQAKEHQQFITFLQRLLGPLLEAYSSAAIFVHTFRGAVPESEYLQKLHRYLLTRTERNVAVYAESATYCLVKNAVKMFKDIGVFKETKQKRASVLELSTTFLPQGSRQKLLEYILSFVVL

Target ORF information:

RefSeq Version	XM_006231625.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231625.3

ORF Insert Sequence:

ATGGAGGAGT CTTCAGTGAC AATTGGCACA ATAGACGTTT CTTATCTGCC CAATTCATCA 
GAATACAGCC TTGGCCGATG TAAACACACG AATGAGGACT GGGTTGACTG TGGCTTCAAA 
CCTACCTTCT TCAGATCCGC AACGCTGAAA TGGAAGGAGA GCCTCATGAG CCGGAAGAGG 
CCCTTCGTGG GAAGGTGTTG CTATTCATGC ACGCCTCAGA GCTGGGAAAG GTTTTTCAAC 
CCCAGTATCC CATCTCTGGG TTTGCGGAAT GTTATTTATA TCAATGAAAC TCACACAAGG 
CACCGAGGAT GGCTGGCAAG ACGGCTTTCT TACATCCTTT TTGTTCAAGA GCGCGATGTC 
CACAAGGGCA TGTTTGCCAC CAGTATCACT GACAATGTAC TGAATAGCAG CAGAGTCCAA 
GAGGCAATTG CTGAAGTGGC TGCAGAATTG AACCCGGATG GATCTGCCCA GCAGCAGTCC 
AAAGCCATCC AGAAAGTGAA AAGGAAAGCC AGGAAGATCC TTCAGGAAAT GGTTGCTACA 
GTCTCCCCCG GGATGATCAG GCTGACTGGC TGGGTGTTAC TAAAGCTCTT CAACAGCTTC 
TTCTGGAACA TTCAGATTCA CAAGGGTCAA CTTGAGATGG TGAAAGCTGC AACTGAGACG 
AATCTGCCGC TCTTGTTTCT GCCGGTGCAC AGATCCCACA TCGACTACCT GCTGCTCACC 
TTCATCCTCT TCTGCCACAA CATCAAAGCT CCATACATCG CCTCGGGCAA CAACCTCAAC 
ATCCCCATCT TCAGTACCTT GATTCACAAG CTTGGGGGCT TTTTCATAAG ACGGAGGCTT 
GACGAAACTC CAGATGGACG CAAAGACATT CTGTACAGAG CGTTGCTCCA TGGGCATATA 
GTTGAACTCC TCCGACAGCA GCAGTTCCTG GAGATCTTCC TGGAAGGCAC CCGCTCCCGC 
AGTGGCAAGA CCTCCTGTGC CCGGGCCGGG CTCCTGTCAG TGGTAGTGGA TACTCTGTCA 
TCCAACACCA TCCCTGACAT CCTGGTCATC CCTGTGGGCA TCTCGTATGA TCGGATAATC 
GAAGGTCACT ACAATGGTGA ACAGTTGGGC AAGCCCAAGA AGAATGAAAG TCTCTGGAGT 
GTGGCAAGAG GCGTTATCAG AATGCTGCGG AAAAACTACG GCTATGTCCG AGTGGACTTT 
GCACAGCCAT TTTCTTTGAA GGAATATTTA GAAGGCCAAA GTCAGAAACC TGTATCTGCT 
CCCCTCTCTT TGGAGCAAGC ACTGTTACCA GCAATCCTTC CTTCAAGACC TGATGCTGCT 
GCTGCCGAAC ATGAAGACAT GTCCAGTAAT GAGTCGAGAA ACGCGGCAGA CGAAGCCTTC 
CGAAGGAGGC TGATCACAAA CCTGGCGGAG CACATTCTCT TCACCGCCAG CAAGTCCTGC 
GCTATCATGT CCACCCACAT TGTGGCCTGC CTGCTCCTCT ACAGACACAG GCAGGGAATC 
CACCTCTCCA CGCTGGTGGA AGACTTCTTT GTGATGAAGG AGGAAGTCCT AGCTCGGGAT 
TTTGACCTGG GCTTCTCCGG GAATTCAGAA GACGTAGTCA TGCATGCTAT TCAGCTTCTG 
GGGAACTGTG TCACAATCAC CCACACTAGC AGGAAGGATG AATTCTTTAT TACTCCCAGC 
ACAACTGTCC CGTCCGTCTT TGAACTCAAC TTCTACAGCA ATGGGGTACT TCATGTCTTT 
ATCATGGAAG CCATCATAGC TTGCAGCATT TATGCAGTCC AGAATAAGAG GGGTTCCGGA 
GGGTCTGCCG GAGGCCTTGG CAACCTGATC AGCCAGGAGC AGCTGGTGCG GAAGGCCGCC 
AGTCTGTGCT ACCTTCTCTC TAATGAAGGT ACCATTTCTC TGCCCTGCCA GACATTTTAC 
CAGGTTTGTC ACGAGACAGT AGGAAAGTTC ATCCAGTACG GAATTCTCAC AGTGGCAGAG 
CAAGATGACC AGGAAGATGT CAGTCCTGGC CTTGCAGAGC AGCAGTGGAA CAAGAAGCTT 
CCGGAGCCTC TGAACTGGAG AAGTGACGAA GAAGATGAGG ACAGTGACTT TGGTGAGGAG 
CAGCGTGATT GCTACCTGAA GGTGAGCCAG GCCAAGGAGC ACCAGCAATT CATCACCTTT 
CTGCAGAGGC TTCTGGGGCC CCTGCTAGAA GCCTACAGCT CTGCTGCCAT CTTTGTCCAC 
ACCTTCCGCG GCGCAGTCCC GGAGTCTGAG TACCTGCAGA AGCTGCACAG GTACCTTCTC 
ACCAGGACGG AGAGGAACGT CGCGGTGTAC GCTGAGAGTG CCACATACTG TCTTGTGAAG 
AATGCTGTGA AAATGTTTAA GGACATCGGG GTTTTCAAAG AGACCAAGCA GAAGCGAGCG 
TCTGTCTTAG AACTGAGCAC CACTTTCCTA CCTCAGGGCA GCCGGCAGAA GCTCCTGGAA 
TACATTCTGA GCTTCGTGGT GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptional regulation of mitochondrial glycerophosphate acyltransferase is mediated by distal promoter via ChREBP and SREBP-1.
Archives of biochemistry and biophysics490(2)85-95(2009 Oct)
Guha P,Aneja KK,Shilpi RY,Haldar D

Glycerol-3-phosphate acyltransferase 1 is essential for the immune response to infection with coxsackievirus B3 in mice.
The Journal of nutrition139(4)779-83(2009 Apr)
Karlsson EA,Wang S,Shi Q,Coleman RA,Beck MA

Atorvastatin prevents carbohydrate response element binding protein activation in the fructose-fed rat by activating protein kinase A.
Hepatology (Baltimore, Md.)49(1)106-15(2009 Jan)
Rodr?guez-Calvo R,Barroso E,Serrano L,Coll T,S?nchez RM,Merlos M,Palomer X,Laguna JC,V?zquez-Carrera M

Glycerol-3-phosphate acyltransferase-1 regulates murine T-lymphocyte proliferation and cytokine production.
American journal of physiology. Cell physiology295(6)C1543-9(2008 Dec)
Collison LW,Murphy EJ,Jolly CA

Expression profiling of hepatic genes associated with lipid metabolism in nephrotic rats.
American journal of physiology. Renal physiology295(3)F662-71(2008 Sep)
Zhou Y,Zhang X,Chen L,Wu J,Dang H,Wei M,Fan Y,Zhang Y,Zhu Y,Wang N,Breyer MD,Guan Y

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Gpam cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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