Mcm8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mcm8
Entrez Gene ID	296178
Full Name	minichromosome maintenance 8 homologous recombination repair factor
General protein information	Preferred Names minichromosome maintenance 8 homologous recombination repair factor Names DNA helicase MCM8 DNA replication licensing factor MCM8 minichromosome maintenance complex component 8 minichromosome maintenance deficient 8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_006235074.3	XP_006235136.1	DNA helicase MCM8 isoform X1
NM_001106514.1	NP_001099984.1	DNA helicase MCM8
XM_008762163.2	XP_008760385.1	DNA helicase MCM8 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MCM8	XP_534352.2
Mus musculus (house mouse)	Mcm8	NP_079952.2
Gallus gallus (chicken)	MCM8	XP_004935348.1
Pan troglodytes (chimpanzee)	MCM8	XP_001166933.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002580	XP_312356.3
Homo sapiens (human)	MCM8	NP_001268449.1
Arabidopsis thaliana (thale cress)	MCM8	NP_187577.1
Rattus norvegicus (Norway rat)	Mcm8	NP_001099984.1
Danio rerio (zebrafish)	LOC100333576	XP_002665161.2
Xenopus tropicalis (tropical clawed frog)	mcm8	NP_001072344.1
	MCM8	XP_001115843.1
Bos taurus (cattle)	MCM8	NP_001179965.1
Oryza sativa (rice)	Os05g0464100	NP_001055777.1

Gene Ontology
Bibliography

Related articles in PubMed

MCM9 Is Required for Mammalian DNA Mismatch Repair.
Traver S, Coulombe P, Peiffer I, Hutchins JR, Kitzmann M, Latreille D, M?chali M
Molecular cell59(5)831-9(2015 Sep)

MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex.
Lee KY, Im JS, Shibata E, Park J, Handa N, Kowalczykowski SC, Dutta A
Nature communications67744(2015 Jul)

The MCM8-MCM9 complex promotes RAD51 recruitment at DNA damage sites to facilitate homologous recombination.
Park J, Long DT, Lee KY, Abbas T, Shibata E, Negishi M, Luo Y, Schimenti JC, Gambus A, Walter JC, Dutta A
Molecular and cellular biology33(8)1632-44(2013 Apr)

MCM8- and MCM9-deficient mice reveal gametogenesis defects and genome instability due to impaired homologous recombination.
Lutzmann M, Grey C, Traver S, Ganier O, Maya-Mendoza A, Ranisavljevic N, Bernex F, Nishiyama A, Montel N, Gavois E, Forichon L, de Massy B, M?chali M
Molecular cell47(4)523-34(2012 Aug)

Mcm8 and Mcm9 form a complex that functions in homologous recombination repair induced by DNA interstrand crosslinks.
Nishimura K, Ishiai M, Horikawa K, Fukagawa T, Takata M, Takisawa H, Kanemaki MT
Molecular cell47(4)511-22(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mcm8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mcm8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04814	NM_001106514.1 Latest version!	Rattus norvegicus minichromosome maintenance 8 homologous recombination repair factor (Mcm8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa04814	XM_008762163.2 Latest version!	Rattus norvegicus minichromosome maintenance 8 homologous recombination repair factor (Mcm8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa04814	XM_006235074.3 Latest version!	Rattus norvegicus minichromosome maintenance 8 homologous recombination repair factor (Mcm8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mcm8 ( NM_001106514.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099984.1 Rattus norvegicus minichromosome maintenance 8 homologous recombination repair factor (Mcm8), mRNA.

Price & Availability↑

CloneID	ORa04814
Clone ID Related Accession (Same CDS sequence)	XM_006235074.3 , NM_001106514.1 , XM_008762163.2
Accession Version	NM_001106514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-06
Organism	Rattus norvegicus(Norway rat)
Product	DNA helicase MCM8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473949.1. On or before Oct 4, 2007 this sequence version replaced XM_001079873.1, XM_215825.4. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052297, SAMN04563757 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGGTG CATACAGAGG CAGAGGTTTT GGACGAGGAC GATTCCAAAA CTGGAAAAGA 
GGAAGAGGTG GTGGGAACTT CTCAGGAAGG TGGAGAGACA GAACTGACCT GAGTAAAGCT 
GCAGGAAATC ATGCTTCTGA ACAAGCCTCA CAACCTTTAC TTCAACAGTC AACATTGGAT 
CAGTTTATAC CGTATAAGGG CTGGAAGCTT TACTTCTCTG AAGTTTACAG TAATAACTCT 
CCTCTTACTG AGAAGATCCA AGCATTTGAG AAATTTTTCA CAAGGCATAT CGACTTATAT 
GATAAGGATG AAATAGAAAG AAAGGGAAGC ATCTTGGTGG ATTTTAAAGA ACTGACAAAA 
GACAATGAAA TAACTAACTT GATACCAGAT ATAGAAAATG CACTAAGAGA TGCCCCTGAG 
AAAACACTGG CATGTATGGG ACTGGCAATA CATCAGGTAT TAACTAAGGA CCTTGAAAGG 
CACGCAGCTG AATTACAAGC TCAAGAGGGG TTGTGTAATG GTGGAGGAAC CATGGTAAAT 
GTGCCACATA TTTATGCGAG AGTGTACAAC TATGAGCCCT TAACACACCT CAAGAACATC 
CGAGCAACTT GCTATGGGAA GTACATCTCT ATAAGAGGGA CAGTGGTTCG AGTCAGTAAC 
ATAAAGCCTC TGTGCACCAA AATGGCTTTT CAGTGTGCTG CATGTGGAGA GATTCAGAGC 
TTTCCTCTTC CGGATGGAAA ATACAATCTT CCAACAAAGT GTCCTGTGCC TGCGTGTCGA 
GGGAGGTCAT TTACGCCATT GCGCAGTTCT CCTCTCACCG TCACAATGGA CTGGCAGTTG 
ATCAAAATCC AGGAGCTGAT GTCTGATGCA CAGAGAGAAG CTGGTCGGAT CCCTCGAACC 
ATAGAATGTG AACTTGTTCA TGATCTTGTA GATAGCTGTG TCCCAGGAGA TACGGTGACT 
GTTACTGGAA TTGTCAAAGT TTCAAATAGT GAAGAAGGTT CTCGATCTAA GAATGATAAG 
TGCATGTTCC TTTTGTACAT TGAAGCAAAC TCTGTTAGCA ACAGCAAAGG ACAGAAAGCC 
CAGACTGCAG AGGATGGTTG CAAGCATGGA ACACTGATGG AGTTCTCCCT TAAAGACCTG 
TATGCCATCC AAGAGATCCA GGCTGAAGAG AACCTGCTCA AGCTCATTGT CAACTCACTT 
TGTCCTGTCA TTTTTGGTCA TGAACTTGTT AAAGCAGGAC TGATGTTAGC GCTATTTGGC 
GGTAGCCAGA AGTACGCAGA TGACAAAAAC AGGATTCCTA TTCGAGGAGA CCCACACGTC 
CTGATTGTTG GAGATCCAGG CTTGGGGAAG AGTCAGATGC TGCAGGCAGC ATGCAACGTG 
GCGCCACGTG GTGTGTATGT TTGTGGAAAT ACGGCCACCA GCTCTGGTCT GACGGTTACT 
CTTTCAAAAG ACAGTTCCTC AGGAGACTTC GCTTTGGAAG CTGGTGCCCT GGTACTTGGT 
GACCAAGGCA TTTGTGGAAT AGATGAATTT GATAAAATGG GGAATCAGCA TCAAGCCTTG 
TTAGAAGCCA TGGAACAGCA GAGCATTAGC CTTGCTAAGG CCGGTGTGGT TTGCAGCCTC 
CCTGCAAGAA CCTCCATTAT TGCTGCGGCA AATCCAGTCG GAGGACACTA CAATAAAGCG 
AGGACAGTTT CTGAGAATTT AAAGATGGGG AGTGCCCTGC TGTCCAGGTT CGATTTGGTC 
TTTATCTTGT TAGATACACC AAATGAACAG CACGACCACT TACTTTCTGA ACATGTGATC 
GCAATAAGAG CTGGGAAGCA GAGAGCGGTT AGCAGTGCCA CAGTCTCTCG TGTGCTCAGT 
CAAGACTCAA ATACTTCTGT ACTTGAAGTG GTTTCCGAGA AACCGTTATC AGAAAGACTA 
AAGGTGGCTC CTGGAGAAAA GACAGATCCG ATTCCACATC AGCTGCTGAG GAAGTACATA 
GGCTATGCAC GGCAGTATGT CCATCCAAGG TTATCCACGG AAGCTGCTCA GGCTCTTCAG 
GATTTCTACC TGGAGCTGCG CAAGCAGAGC CAGCGCGTGG GCAGCTCGCC CATCACCACT 
CGGCAGCTGG AATCTTTGAT CCGTCTGACG GAGGCACGAG CAAGGTTAGA ATTGAGAGAG 
GAAGCAACTA AAGAAGACGC TGAAGATATA ATTGAAATTA TGAAGCATAG CATGCTAGGA 
ACTTACTCAG ATGAATTTGG AAACCTGGAC TTTGAGCGAT CCCAGCATGG CTCTGGAATG 
AGCAACAGGT CAACAGCTAA AAGATTTATT TCTGCTCTCA ACAGCATTGC TGAAAGAACG 
TATAACAACA TATTTCAATT TCATCAACTT CGTCAGATTG CTAAAGAGCT AAACATTCAG 
GTTGCTGACT TTGAAAACTT CATTGGATCA CTGAATGACC AGGGTTATCT CCTGAAAAAA 
GGCCCAAAGA TTTATCAACT TCAAACTATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099984.1
CDS	291..2783
Translation	MSGAYRGRGFGRGRFQNWKRGRGGGNFSGRWRDRTDLSKAAGNHASEQASQPLLQQSTLDQFIPYKGWKLYFSEVYSNNSPLTEKIQAFEKFFTRHIDLYDKDEIERKGSILVDFKELTKDNEITNLIPDIENALRDAPEKTLACMGLAIHQVLTKDLERHAAELQAQEGLCNGGGTMVNVPHIYARVYNYEPLTHLKNIRATCYGKYISIRGTVVRVSNIKPLCTKMAFQCAACGEIQSFPLPDGKYNLPTKCPVPACRGRSFTPLRSSPLTVTMDWQLIKIQELMSDAQREAGRIPRTIECELVHDLVDSCVPGDTVTVTGIVKVSNSEEGSRSKNDKCMFLLYIEANSVSNSKGQKAQTAEDGCKHGTLMEFSLKDLYAIQEIQAEENLLKLIVNSLCPVIFGHELVKAGLMLALFGGSQKYADDKNRIPIRGDPHVLIVGDPGLGKSQMLQAACNVAPRGVYVCGNTATSSGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKARTVSENLKMGSALLSRFDLVFILLDTPNEQHDHLLSEHVIAIRAGKQRAVSSATVSRVLSQDSNTSVLEVVSEKPLSERLKVAPGEKTDPIPHQLLRKYIGYARQYVHPRLSTEAAQALQDFYLELRKQSQRVGSSPITTRQLESLIRLTEARARLELREEATKEDAEDIIEIMKHSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNSIAERTYNNIFQFHQLRQIAKELNIQVADFENFIGSLNDQGYLLKKGPKIYQLQTM

Target ORF information:

RefSeq Version	NM_001106514.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus minichromosome maintenance 8 homologous recombination repair factor (Mcm8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106514.1

ORF Insert Sequence:

ATGAGTGGTG CATACAGAGG CAGAGGTTTT GGACGAGGAC GATTCCAAAA CTGGAAAAGA 
GGAAGAGGTG GTGGGAACTT CTCAGGAAGG TGGAGAGACA GAACTGACCT GAGTAAAGCT 
GCAGGAAATC ATGCTTCTGA ACAAGCCTCA CAACCTTTAC TTCAACAGTC AACATTGGAT 
CAGTTTATAC CGTATAAGGG CTGGAAGCTT TACTTCTCTG AAGTTTACAG TAATAACTCT 
CCTCTTACTG AGAAGATCCA AGCATTTGAG AAATTTTTCA CAAGGCATAT CGACTTATAT 
GATAAGGATG AAATAGAAAG AAAGGGAAGC ATCTTGGTGG ATTTTAAAGA ACTGACAAAA 
GACAATGAAA TAACTAACTT GATACCAGAT ATAGAAAATG CACTAAGAGA TGCCCCTGAG 
AAAACACTGG CATGTATGGG ACTGGCAATA CATCAGGTAT TAACTAAGGA CCTTGAAAGG 
CACGCAGCTG AATTACAAGC TCAAGAGGGG TTGTGTAATG GTGGAGGAAC CATGGTAAAT 
GTGCCACATA TTTATGCGAG AGTGTACAAC TATGAGCCCT TAACACACCT CAAGAACATC 
CGAGCAACTT GCTATGGGAA GTACATCTCT ATAAGAGGGA CAGTGGTTCG AGTCAGTAAC 
ATAAAGCCTC TGTGCACCAA AATGGCTTTT CAGTGTGCTG CATGTGGAGA GATTCAGAGC 
TTTCCTCTTC CGGATGGAAA ATACAATCTT CCAACAAAGT GTCCTGTGCC TGCGTGTCGA 
GGGAGGTCAT TTACGCCATT GCGCAGTTCT CCTCTCACCG TCACAATGGA CTGGCAGTTG 
ATCAAAATCC AGGAGCTGAT GTCTGATGCA CAGAGAGAAG CTGGTCGGAT CCCTCGAACC 
ATAGAATGTG AACTTGTTCA TGATCTTGTA GATAGCTGTG TCCCAGGAGA TACGGTGACT 
GTTACTGGAA TTGTCAAAGT TTCAAATAGT GAAGAAGGTT CTCGATCTAA GAATGATAAG 
TGCATGTTCC TTTTGTACAT TGAAGCAAAC TCTGTTAGCA ACAGCAAAGG ACAGAAAGCC 
CAGACTGCAG AGGATGGTTG CAAGCATGGA ACACTGATGG AGTTCTCCCT TAAAGACCTG 
TATGCCATCC AAGAGATCCA GGCTGAAGAG AACCTGCTCA AGCTCATTGT CAACTCACTT 
TGTCCTGTCA TTTTTGGTCA TGAACTTGTT AAAGCAGGAC TGATGTTAGC GCTATTTGGC 
GGTAGCCAGA AGTACGCAGA TGACAAAAAC AGGATTCCTA TTCGAGGAGA CCCACACGTC 
CTGATTGTTG GAGATCCAGG CTTGGGGAAG AGTCAGATGC TGCAGGCAGC ATGCAACGTG 
GCGCCACGTG GTGTGTATGT TTGTGGAAAT ACGGCCACCA GCTCTGGTCT GACGGTTACT 
CTTTCAAAAG ACAGTTCCTC AGGAGACTTC GCTTTGGAAG CTGGTGCCCT GGTACTTGGT 
GACCAAGGCA TTTGTGGAAT AGATGAATTT GATAAAATGG GGAATCAGCA TCAAGCCTTG 
TTAGAAGCCA TGGAACAGCA GAGCATTAGC CTTGCTAAGG CCGGTGTGGT TTGCAGCCTC 
CCTGCAAGAA CCTCCATTAT TGCTGCGGCA AATCCAGTCG GAGGACACTA CAATAAAGCG 
AGGACAGTTT CTGAGAATTT AAAGATGGGG AGTGCCCTGC TGTCCAGGTT CGATTTGGTC 
TTTATCTTGT TAGATACACC AAATGAACAG CACGACCACT TACTTTCTGA ACATGTGATC 
GCAATAAGAG CTGGGAAGCA GAGAGCGGTT AGCAGTGCCA CAGTCTCTCG TGTGCTCAGT 
CAAGACTCAA ATACTTCTGT ACTTGAAGTG GTTTCCGAGA AACCGTTATC AGAAAGACTA 
AAGGTGGCTC CTGGAGAAAA GACAGATCCG ATTCCACATC AGCTGCTGAG GAAGTACATA 
GGCTATGCAC GGCAGTATGT CCATCCAAGG TTATCCACGG AAGCTGCTCA GGCTCTTCAG 
GATTTCTACC TGGAGCTGCG CAAGCAGAGC CAGCGCGTGG GCAGCTCGCC CATCACCACT 
CGGCAGCTGG AATCTTTGAT CCGTCTGACG GAGGCACGAG CAAGGTTAGA ATTGAGAGAG 
GAAGCAACTA AAGAAGACGC TGAAGATATA ATTGAAATTA TGAAGCATAG CATGCTAGGA 
ACTTACTCAG ATGAATTTGG AAACCTGGAC TTTGAGCGAT CCCAGCATGG CTCTGGAATG 
AGCAACAGGT CAACAGCTAA AAGATTTATT TCTGCTCTCA ACAGCATTGC TGAAAGAACG 
TATAACAACA TATTTCAATT TCATCAACTT CGTCAGATTG CTAAAGAGCT AAACATTCAG 
GTTGCTGACT TTGAAAACTT CATTGGATCA CTGAATGACC AGGGTTATCT CCTGAAAAAA 
GGCCCAAAGA TTTATCAACT TCAAACTATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcm8 ( XM_006235074.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235136.1 Rattus norvegicus minichromosome maintenance 8 homologous recombination repair factor (Mcm8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa04814
Clone ID Related Accession (Same CDS sequence)	XM_006235074.3 , NM_001106514.1 , XM_008762163.2
Accession Version	XM_006235074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA helicase MCM8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGTG CATACAGAGG CAGAGGTTTT GGACGAGGAC GATTCCAAAA CTGGAAAAGA 
GGAAGAGGTG GTGGGAACTT CTCAGGAAGG TGGAGAGACA GAACTGACCT GAGTAAAGCT 
GCAGGAAATC ATGCTTCTGA ACAAGCCTCA CAACCTTTAC TTCAACAGTC AACATTGGAT 
CAGTTTATAC CGTATAAGGG CTGGAAGCTT TACTTCTCTG AAGTTTACAG TAATAACTCT 
CCTCTTACTG AGAAGATCCA AGCATTTGAG AAATTTTTCA CAAGGCATAT CGACTTATAT 
GATAAGGATG AAATAGAAAG AAAGGGAAGC ATCTTGGTGG ATTTTAAAGA ACTGACAAAA 
GACAATGAAA TAACTAACTT GATACCAGAT ATAGAAAATG CACTAAGAGA TGCCCCTGAG 
AAAACACTGG CATGTATGGG ACTGGCAATA CATCAGGTAT TAACTAAGGA CCTTGAAAGG 
CACGCAGCTG AATTACAAGC TCAAGAGGGG TTGTGTAATG GTGGAGGAAC CATGGTAAAT 
GTGCCACATA TTTATGCGAG AGTGTACAAC TATGAGCCCT TAACACACCT CAAGAACATC 
CGAGCAACTT GCTATGGGAA GTACATCTCT ATAAGAGGGA CAGTGGTTCG AGTCAGTAAC 
ATAAAGCCTC TGTGCACCAA AATGGCTTTT CAGTGTGCTG CATGTGGAGA GATTCAGAGC 
TTTCCTCTTC CGGATGGAAA ATACAATCTT CCAACAAAGT GTCCTGTGCC TGCGTGTCGA 
GGGAGGTCAT TTACGCCATT GCGCAGTTCT CCTCTCACCG TCACAATGGA CTGGCAGTTG 
ATCAAAATCC AGGAGCTGAT GTCTGATGCA CAGAGAGAAG CTGGTCGGAT CCCTCGAACC 
ATAGAATGTG AACTTGTTCA TGATCTTGTA GATAGCTGTG TCCCAGGAGA TACGGTGACT 
GTTACTGGAA TTGTCAAAGT TTCAAATAGT GAAGAAGGTT CTCGATCTAA GAATGATAAG 
TGCATGTTCC TTTTGTACAT TGAAGCAAAC TCTGTTAGCA ACAGCAAAGG ACAGAAAGCC 
CAGACTGCAG AGGATGGTTG CAAGCATGGA ACACTGATGG AGTTCTCCCT TAAAGACCTG 
TATGCCATCC AAGAGATCCA GGCTGAAGAG AACCTGCTCA AGCTCATTGT CAACTCACTT 
TGTCCTGTCA TTTTTGGTCA TGAACTTGTT AAAGCAGGAC TGATGTTAGC GCTATTTGGC 
GGTAGCCAGA AGTACGCAGA TGACAAAAAC AGGATTCCTA TTCGAGGAGA CCCACACGTC 
CTGATTGTTG GAGATCCAGG CTTGGGGAAG AGTCAGATGC TGCAGGCAGC ATGCAACGTG 
GCGCCACGTG GTGTGTATGT TTGTGGAAAT ACGGCCACCA GCTCTGGTCT GACGGTTACT 
CTTTCAAAAG ACAGTTCCTC AGGAGACTTC GCTTTGGAAG CTGGTGCCCT GGTACTTGGT 
GACCAAGGCA TTTGTGGAAT AGATGAATTT GATAAAATGG GGAATCAGCA TCAAGCCTTG 
TTAGAAGCCA TGGAACAGCA GAGCATTAGC CTTGCTAAGG CCGGTGTGGT TTGCAGCCTC 
CCTGCAAGAA CCTCCATTAT TGCTGCGGCA AATCCAGTCG GAGGACACTA CAATAAAGCG 
AGGACAGTTT CTGAGAATTT AAAGATGGGG AGTGCCCTGC TGTCCAGGTT CGATTTGGTC 
TTTATCTTGT TAGATACACC AAATGAACAG CACGACCACT TACTTTCTGA ACATGTGATC 
GCAATAAGAG CTGGGAAGCA GAGAGCGGTT AGCAGTGCCA CAGTCTCTCG TGTGCTCAGT 
CAAGACTCAA ATACTTCTGT ACTTGAAGTG GTTTCCGAGA AACCGTTATC AGAAAGACTA 
AAGGTGGCTC CTGGAGAAAA GACAGATCCG ATTCCACATC AGCTGCTGAG GAAGTACATA 
GGCTATGCAC GGCAGTATGT CCATCCAAGG TTATCCACGG AAGCTGCTCA GGCTCTTCAG 
GATTTCTACC TGGAGCTGCG CAAGCAGAGC CAGCGCGTGG GCAGCTCGCC CATCACCACT 
CGGCAGCTGG AATCTTTGAT CCGTCTGACG GAGGCACGAG CAAGGTTAGA ATTGAGAGAG 
GAAGCAACTA AAGAAGACGC TGAAGATATA ATTGAAATTA TGAAGCATAG CATGCTAGGA 
ACTTACTCAG ATGAATTTGG AAACCTGGAC TTTGAGCGAT CCCAGCATGG CTCTGGAATG 
AGCAACAGGT CAACAGCTAA AAGATTTATT TCTGCTCTCA ACAGCATTGC TGAAAGAACG 
TATAACAACA TATTTCAATT TCATCAACTT CGTCAGATTG CTAAAGAGCT AAACATTCAG 
GTTGCTGACT TTGAAAACTT CATTGGATCA CTGAATGACC AGGGTTATCT CCTGAAAAAA 
GGCCCAAAGA TTTATCAACT TCAAACTATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235136.1
CDS	242..2734
Translation	MSGAYRGRGFGRGRFQNWKRGRGGGNFSGRWRDRTDLSKAAGNHASEQASQPLLQQSTLDQFIPYKGWKLYFSEVYSNNSPLTEKIQAFEKFFTRHIDLYDKDEIERKGSILVDFKELTKDNEITNLIPDIENALRDAPEKTLACMGLAIHQVLTKDLERHAAELQAQEGLCNGGGTMVNVPHIYARVYNYEPLTHLKNIRATCYGKYISIRGTVVRVSNIKPLCTKMAFQCAACGEIQSFPLPDGKYNLPTKCPVPACRGRSFTPLRSSPLTVTMDWQLIKIQELMSDAQREAGRIPRTIECELVHDLVDSCVPGDTVTVTGIVKVSNSEEGSRSKNDKCMFLLYIEANSVSNSKGQKAQTAEDGCKHGTLMEFSLKDLYAIQEIQAEENLLKLIVNSLCPVIFGHELVKAGLMLALFGGSQKYADDKNRIPIRGDPHVLIVGDPGLGKSQMLQAACNVAPRGVYVCGNTATSSGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKARTVSENLKMGSALLSRFDLVFILLDTPNEQHDHLLSEHVIAIRAGKQRAVSSATVSRVLSQDSNTSVLEVVSEKPLSERLKVAPGEKTDPIPHQLLRKYIGYARQYVHPRLSTEAAQALQDFYLELRKQSQRVGSSPITTRQLESLIRLTEARARLELREEATKEDAEDIIEIMKHSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNSIAERTYNNIFQFHQLRQIAKELNIQVADFENFIGSLNDQGYLLKKGPKIYQLQTM

Target ORF information:

RefSeq Version	XM_006235074.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus minichromosome maintenance 8 homologous recombination repair factor (Mcm8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235074.3

ORF Insert Sequence:

ATGAGTGGTG CATACAGAGG CAGAGGTTTT GGACGAGGAC GATTCCAAAA CTGGAAAAGA 
GGAAGAGGTG GTGGGAACTT CTCAGGAAGG TGGAGAGACA GAACTGACCT GAGTAAAGCT 
GCAGGAAATC ATGCTTCTGA ACAAGCCTCA CAACCTTTAC TTCAACAGTC AACATTGGAT 
CAGTTTATAC CGTATAAGGG CTGGAAGCTT TACTTCTCTG AAGTTTACAG TAATAACTCT 
CCTCTTACTG AGAAGATCCA AGCATTTGAG AAATTTTTCA CAAGGCATAT CGACTTATAT 
GATAAGGATG AAATAGAAAG AAAGGGAAGC ATCTTGGTGG ATTTTAAAGA ACTGACAAAA 
GACAATGAAA TAACTAACTT GATACCAGAT ATAGAAAATG CACTAAGAGA TGCCCCTGAG 
AAAACACTGG CATGTATGGG ACTGGCAATA CATCAGGTAT TAACTAAGGA CCTTGAAAGG 
CACGCAGCTG AATTACAAGC TCAAGAGGGG TTGTGTAATG GTGGAGGAAC CATGGTAAAT 
GTGCCACATA TTTATGCGAG AGTGTACAAC TATGAGCCCT TAACACACCT CAAGAACATC 
CGAGCAACTT GCTATGGGAA GTACATCTCT ATAAGAGGGA CAGTGGTTCG AGTCAGTAAC 
ATAAAGCCTC TGTGCACCAA AATGGCTTTT CAGTGTGCTG CATGTGGAGA GATTCAGAGC 
TTTCCTCTTC CGGATGGAAA ATACAATCTT CCAACAAAGT GTCCTGTGCC TGCGTGTCGA 
GGGAGGTCAT TTACGCCATT GCGCAGTTCT CCTCTCACCG TCACAATGGA CTGGCAGTTG 
ATCAAAATCC AGGAGCTGAT GTCTGATGCA CAGAGAGAAG CTGGTCGGAT CCCTCGAACC 
ATAGAATGTG AACTTGTTCA TGATCTTGTA GATAGCTGTG TCCCAGGAGA TACGGTGACT 
GTTACTGGAA TTGTCAAAGT TTCAAATAGT GAAGAAGGTT CTCGATCTAA GAATGATAAG 
TGCATGTTCC TTTTGTACAT TGAAGCAAAC TCTGTTAGCA ACAGCAAAGG ACAGAAAGCC 
CAGACTGCAG AGGATGGTTG CAAGCATGGA ACACTGATGG AGTTCTCCCT TAAAGACCTG 
TATGCCATCC AAGAGATCCA GGCTGAAGAG AACCTGCTCA AGCTCATTGT CAACTCACTT 
TGTCCTGTCA TTTTTGGTCA TGAACTTGTT AAAGCAGGAC TGATGTTAGC GCTATTTGGC 
GGTAGCCAGA AGTACGCAGA TGACAAAAAC AGGATTCCTA TTCGAGGAGA CCCACACGTC 
CTGATTGTTG GAGATCCAGG CTTGGGGAAG AGTCAGATGC TGCAGGCAGC ATGCAACGTG 
GCGCCACGTG GTGTGTATGT TTGTGGAAAT ACGGCCACCA GCTCTGGTCT GACGGTTACT 
CTTTCAAAAG ACAGTTCCTC AGGAGACTTC GCTTTGGAAG CTGGTGCCCT GGTACTTGGT 
GACCAAGGCA TTTGTGGAAT AGATGAATTT GATAAAATGG GGAATCAGCA TCAAGCCTTG 
TTAGAAGCCA TGGAACAGCA GAGCATTAGC CTTGCTAAGG CCGGTGTGGT TTGCAGCCTC 
CCTGCAAGAA CCTCCATTAT TGCTGCGGCA AATCCAGTCG GAGGACACTA CAATAAAGCG 
AGGACAGTTT CTGAGAATTT AAAGATGGGG AGTGCCCTGC TGTCCAGGTT CGATTTGGTC 
TTTATCTTGT TAGATACACC AAATGAACAG CACGACCACT TACTTTCTGA ACATGTGATC 
GCAATAAGAG CTGGGAAGCA GAGAGCGGTT AGCAGTGCCA CAGTCTCTCG TGTGCTCAGT 
CAAGACTCAA ATACTTCTGT ACTTGAAGTG GTTTCCGAGA AACCGTTATC AGAAAGACTA 
AAGGTGGCTC CTGGAGAAAA GACAGATCCG ATTCCACATC AGCTGCTGAG GAAGTACATA 
GGCTATGCAC GGCAGTATGT CCATCCAAGG TTATCCACGG AAGCTGCTCA GGCTCTTCAG 
GATTTCTACC TGGAGCTGCG CAAGCAGAGC CAGCGCGTGG GCAGCTCGCC CATCACCACT 
CGGCAGCTGG AATCTTTGAT CCGTCTGACG GAGGCACGAG CAAGGTTAGA ATTGAGAGAG 
GAAGCAACTA AAGAAGACGC TGAAGATATA ATTGAAATTA TGAAGCATAG CATGCTAGGA 
ACTTACTCAG ATGAATTTGG AAACCTGGAC TTTGAGCGAT CCCAGCATGG CTCTGGAATG 
AGCAACAGGT CAACAGCTAA AAGATTTATT TCTGCTCTCA ACAGCATTGC TGAAAGAACG 
TATAACAACA TATTTCAATT TCATCAACTT CGTCAGATTG CTAAAGAGCT AAACATTCAG 
GTTGCTGACT TTGAAAACTT CATTGGATCA CTGAATGACC AGGGTTATCT CCTGAAAAAA 
GGCCCAAAGA TTTATCAACT TCAAACTATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcm8 ( XM_008762163.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760385.1 Rattus norvegicus minichromosome maintenance 8 homologous recombination repair factor (Mcm8), transcript variant X2, mRNA.

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CloneID	ORa04814
Clone ID Related Accession (Same CDS sequence)	XM_006235074.3 , NM_001106514.1 , XM_008762163.2
Accession Version	XM_008762163.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA helicase MCM8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGTG CATACAGAGG CAGAGGTTTT GGACGAGGAC GATTCCAAAA CTGGAAAAGA 
GGAAGAGGTG GTGGGAACTT CTCAGGAAGG TGGAGAGACA GAACTGACCT GAGTAAAGCT 
GCAGGAAATC ATGCTTCTGA ACAAGCCTCA CAACCTTTAC TTCAACAGTC AACATTGGAT 
CAGTTTATAC CGTATAAGGG CTGGAAGCTT TACTTCTCTG AAGTTTACAG TAATAACTCT 
CCTCTTACTG AGAAGATCCA AGCATTTGAG AAATTTTTCA CAAGGCATAT CGACTTATAT 
GATAAGGATG AAATAGAAAG AAAGGGAAGC ATCTTGGTGG ATTTTAAAGA ACTGACAAAA 
GACAATGAAA TAACTAACTT GATACCAGAT ATAGAAAATG CACTAAGAGA TGCCCCTGAG 
AAAACACTGG CATGTATGGG ACTGGCAATA CATCAGGTAT TAACTAAGGA CCTTGAAAGG 
CACGCAGCTG AATTACAAGC TCAAGAGGGG TTGTGTAATG GTGGAGGAAC CATGGTAAAT 
GTGCCACATA TTTATGCGAG AGTGTACAAC TATGAGCCCT TAACACACCT CAAGAACATC 
CGAGCAACTT GCTATGGGAA GTACATCTCT ATAAGAGGGA CAGTGGTTCG AGTCAGTAAC 
ATAAAGCCTC TGTGCACCAA AATGGCTTTT CAGTGTGCTG CATGTGGAGA GATTCAGAGC 
TTTCCTCTTC CGGATGGAAA ATACAATCTT CCAACAAAGT GTCCTGTGCC TGCGTGTCGA 
GGGAGGTCAT TTACGCCATT GCGCAGTTCT CCTCTCACCG TCACAATGGA CTGGCAGTTG 
ATCAAAATCC AGGAGCTGAT GTCTGATGCA CAGAGAGAAG CTGGTCGGAT CCCTCGAACC 
ATAGAATGTG AACTTGTTCA TGATCTTGTA GATAGCTGTG TCCCAGGAGA TACGGTGACT 
GTTACTGGAA TTGTCAAAGT TTCAAATAGT GAAGAAGGTT CTCGATCTAA GAATGATAAG 
TGCATGTTCC TTTTGTACAT TGAAGCAAAC TCTGTTAGCA ACAGCAAAGG ACAGAAAGCC 
CAGACTGCAG AGGATGGTTG CAAGCATGGA ACACTGATGG AGTTCTCCCT TAAAGACCTG 
TATGCCATCC AAGAGATCCA GGCTGAAGAG AACCTGCTCA AGCTCATTGT CAACTCACTT 
TGTCCTGTCA TTTTTGGTCA TGAACTTGTT AAAGCAGGAC TGATGTTAGC GCTATTTGGC 
GGTAGCCAGA AGTACGCAGA TGACAAAAAC AGGATTCCTA TTCGAGGAGA CCCACACGTC 
CTGATTGTTG GAGATCCAGG CTTGGGGAAG AGTCAGATGC TGCAGGCAGC ATGCAACGTG 
GCGCCACGTG GTGTGTATGT TTGTGGAAAT ACGGCCACCA GCTCTGGTCT GACGGTTACT 
CTTTCAAAAG ACAGTTCCTC AGGAGACTTC GCTTTGGAAG CTGGTGCCCT GGTACTTGGT 
GACCAAGGCA TTTGTGGAAT AGATGAATTT GATAAAATGG GGAATCAGCA TCAAGCCTTG 
TTAGAAGCCA TGGAACAGCA GAGCATTAGC CTTGCTAAGG CCGGTGTGGT TTGCAGCCTC 
CCTGCAAGAA CCTCCATTAT TGCTGCGGCA AATCCAGTCG GAGGACACTA CAATAAAGCG 
AGGACAGTTT CTGAGAATTT AAAGATGGGG AGTGCCCTGC TGTCCAGGTT CGATTTGGTC 
TTTATCTTGT TAGATACACC AAATGAACAG CACGACCACT TACTTTCTGA ACATGTGATC 
GCAATAAGAG CTGGGAAGCA GAGAGCGGTT AGCAGTGCCA CAGTCTCTCG TGTGCTCAGT 
CAAGACTCAA ATACTTCTGT ACTTGAAGTG GTTTCCGAGA AACCGTTATC AGAAAGACTA 
AAGGTGGCTC CTGGAGAAAA GACAGATCCG ATTCCACATC AGCTGCTGAG GAAGTACATA 
GGCTATGCAC GGCAGTATGT CCATCCAAGG TTATCCACGG AAGCTGCTCA GGCTCTTCAG 
GATTTCTACC TGGAGCTGCG CAAGCAGAGC CAGCGCGTGG GCAGCTCGCC CATCACCACT 
CGGCAGCTGG AATCTTTGAT CCGTCTGACG GAGGCACGAG CAAGGTTAGA ATTGAGAGAG 
GAAGCAACTA AAGAAGACGC TGAAGATATA ATTGAAATTA TGAAGCATAG CATGCTAGGA 
ACTTACTCAG ATGAATTTGG AAACCTGGAC TTTGAGCGAT CCCAGCATGG CTCTGGAATG 
AGCAACAGGT CAACAGCTAA AAGATTTATT TCTGCTCTCA ACAGCATTGC TGAAAGAACG 
TATAACAACA TATTTCAATT TCATCAACTT CGTCAGATTG CTAAAGAGCT AAACATTCAG 
GTTGCTGACT TTGAAAACTT CATTGGATCA CTGAATGACC AGGGTTATCT CCTGAAAAAA 
GGCCCAAAGA TTTATCAACT TCAAACTATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760385.1
CDS	274..2766
Translation	MSGAYRGRGFGRGRFQNWKRGRGGGNFSGRWRDRTDLSKAAGNHASEQASQPLLQQSTLDQFIPYKGWKLYFSEVYSNNSPLTEKIQAFEKFFTRHIDLYDKDEIERKGSILVDFKELTKDNEITNLIPDIENALRDAPEKTLACMGLAIHQVLTKDLERHAAELQAQEGLCNGGGTMVNVPHIYARVYNYEPLTHLKNIRATCYGKYISIRGTVVRVSNIKPLCTKMAFQCAACGEIQSFPLPDGKYNLPTKCPVPACRGRSFTPLRSSPLTVTMDWQLIKIQELMSDAQREAGRIPRTIECELVHDLVDSCVPGDTVTVTGIVKVSNSEEGSRSKNDKCMFLLYIEANSVSNSKGQKAQTAEDGCKHGTLMEFSLKDLYAIQEIQAEENLLKLIVNSLCPVIFGHELVKAGLMLALFGGSQKYADDKNRIPIRGDPHVLIVGDPGLGKSQMLQAACNVAPRGVYVCGNTATSSGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKARTVSENLKMGSALLSRFDLVFILLDTPNEQHDHLLSEHVIAIRAGKQRAVSSATVSRVLSQDSNTSVLEVVSEKPLSERLKVAPGEKTDPIPHQLLRKYIGYARQYVHPRLSTEAAQALQDFYLELRKQSQRVGSSPITTRQLESLIRLTEARARLELREEATKEDAEDIIEIMKHSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNSIAERTYNNIFQFHQLRQIAKELNIQVADFENFIGSLNDQGYLLKKGPKIYQLQTM

Target ORF information:

RefSeq Version	XM_008762163.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus minichromosome maintenance 8 homologous recombination repair factor (Mcm8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762163.2

ORF Insert Sequence:

ATGAGTGGTG CATACAGAGG CAGAGGTTTT GGACGAGGAC GATTCCAAAA CTGGAAAAGA 
GGAAGAGGTG GTGGGAACTT CTCAGGAAGG TGGAGAGACA GAACTGACCT GAGTAAAGCT 
GCAGGAAATC ATGCTTCTGA ACAAGCCTCA CAACCTTTAC TTCAACAGTC AACATTGGAT 
CAGTTTATAC CGTATAAGGG CTGGAAGCTT TACTTCTCTG AAGTTTACAG TAATAACTCT 
CCTCTTACTG AGAAGATCCA AGCATTTGAG AAATTTTTCA CAAGGCATAT CGACTTATAT 
GATAAGGATG AAATAGAAAG AAAGGGAAGC ATCTTGGTGG ATTTTAAAGA ACTGACAAAA 
GACAATGAAA TAACTAACTT GATACCAGAT ATAGAAAATG CACTAAGAGA TGCCCCTGAG 
AAAACACTGG CATGTATGGG ACTGGCAATA CATCAGGTAT TAACTAAGGA CCTTGAAAGG 
CACGCAGCTG AATTACAAGC TCAAGAGGGG TTGTGTAATG GTGGAGGAAC CATGGTAAAT 
GTGCCACATA TTTATGCGAG AGTGTACAAC TATGAGCCCT TAACACACCT CAAGAACATC 
CGAGCAACTT GCTATGGGAA GTACATCTCT ATAAGAGGGA CAGTGGTTCG AGTCAGTAAC 
ATAAAGCCTC TGTGCACCAA AATGGCTTTT CAGTGTGCTG CATGTGGAGA GATTCAGAGC 
TTTCCTCTTC CGGATGGAAA ATACAATCTT CCAACAAAGT GTCCTGTGCC TGCGTGTCGA 
GGGAGGTCAT TTACGCCATT GCGCAGTTCT CCTCTCACCG TCACAATGGA CTGGCAGTTG 
ATCAAAATCC AGGAGCTGAT GTCTGATGCA CAGAGAGAAG CTGGTCGGAT CCCTCGAACC 
ATAGAATGTG AACTTGTTCA TGATCTTGTA GATAGCTGTG TCCCAGGAGA TACGGTGACT 
GTTACTGGAA TTGTCAAAGT TTCAAATAGT GAAGAAGGTT CTCGATCTAA GAATGATAAG 
TGCATGTTCC TTTTGTACAT TGAAGCAAAC TCTGTTAGCA ACAGCAAAGG ACAGAAAGCC 
CAGACTGCAG AGGATGGTTG CAAGCATGGA ACACTGATGG AGTTCTCCCT TAAAGACCTG 
TATGCCATCC AAGAGATCCA GGCTGAAGAG AACCTGCTCA AGCTCATTGT CAACTCACTT 
TGTCCTGTCA TTTTTGGTCA TGAACTTGTT AAAGCAGGAC TGATGTTAGC GCTATTTGGC 
GGTAGCCAGA AGTACGCAGA TGACAAAAAC AGGATTCCTA TTCGAGGAGA CCCACACGTC 
CTGATTGTTG GAGATCCAGG CTTGGGGAAG AGTCAGATGC TGCAGGCAGC ATGCAACGTG 
GCGCCACGTG GTGTGTATGT TTGTGGAAAT ACGGCCACCA GCTCTGGTCT GACGGTTACT 
CTTTCAAAAG ACAGTTCCTC AGGAGACTTC GCTTTGGAAG CTGGTGCCCT GGTACTTGGT 
GACCAAGGCA TTTGTGGAAT AGATGAATTT GATAAAATGG GGAATCAGCA TCAAGCCTTG 
TTAGAAGCCA TGGAACAGCA GAGCATTAGC CTTGCTAAGG CCGGTGTGGT TTGCAGCCTC 
CCTGCAAGAA CCTCCATTAT TGCTGCGGCA AATCCAGTCG GAGGACACTA CAATAAAGCG 
AGGACAGTTT CTGAGAATTT AAAGATGGGG AGTGCCCTGC TGTCCAGGTT CGATTTGGTC 
TTTATCTTGT TAGATACACC AAATGAACAG CACGACCACT TACTTTCTGA ACATGTGATC 
GCAATAAGAG CTGGGAAGCA GAGAGCGGTT AGCAGTGCCA CAGTCTCTCG TGTGCTCAGT 
CAAGACTCAA ATACTTCTGT ACTTGAAGTG GTTTCCGAGA AACCGTTATC AGAAAGACTA 
AAGGTGGCTC CTGGAGAAAA GACAGATCCG ATTCCACATC AGCTGCTGAG GAAGTACATA 
GGCTATGCAC GGCAGTATGT CCATCCAAGG TTATCCACGG AAGCTGCTCA GGCTCTTCAG 
GATTTCTACC TGGAGCTGCG CAAGCAGAGC CAGCGCGTGG GCAGCTCGCC CATCACCACT 
CGGCAGCTGG AATCTTTGAT CCGTCTGACG GAGGCACGAG CAAGGTTAGA ATTGAGAGAG 
GAAGCAACTA AAGAAGACGC TGAAGATATA ATTGAAATTA TGAAGCATAG CATGCTAGGA 
ACTTACTCAG ATGAATTTGG AAACCTGGAC TTTGAGCGAT CCCAGCATGG CTCTGGAATG 
AGCAACAGGT CAACAGCTAA AAGATTTATT TCTGCTCTCA ACAGCATTGC TGAAAGAACG 
TATAACAACA TATTTCAATT TCATCAACTT CGTCAGATTG CTAAAGAGCT AAACATTCAG 
GTTGCTGACT TTGAAAACTT CATTGGATCA CTGAATGACC AGGGTTATCT CCTGAAAAAA 
GGCCCAAAGA TTTATCAACT TCAAACTATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MCM9 Is Required for Mammalian DNA Mismatch Repair.
Molecular cell59(5)831-9(2015 Sep)
Traver S,Coulombe P,Peiffer I,Hutchins JR,Kitzmann M,Latreille D,M?chali M

MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex.
Nature communications67744(2015 Jul)
Lee KY,Im JS,Shibata E,Park J,Handa N,Kowalczykowski SC,Dutta A

The MCM8-MCM9 complex promotes RAD51 recruitment at DNA damage sites to facilitate homologous recombination.
Molecular and cellular biology33(8)1632-44(2013 Apr)
Park J,Long DT,Lee KY,Abbas T,Shibata E,Negishi M,Luo Y,Schimenti JC,Gambus A,Walter JC,Dutta A

MCM8- and MCM9-deficient mice reveal gametogenesis defects and genome instability due to impaired homologous recombination.
Molecular cell47(4)523-34(2012 Aug)
Lutzmann M,Grey C,Traver S,Ganier O,Maya-Mendoza A,Ranisavljevic N,Bernex F,Nishiyama A,Montel N,Gavois E,Forichon L,de Massy B,M?chali M

Mcm8 and Mcm9 form a complex that functions in homologous recombination repair induced by DNA interstrand crosslinks.
Molecular cell47(4)511-22(2012 Aug)
Nishimura K,Ishiai M,Horikawa K,Fukagawa T,Takata M,Takisawa H,Kanemaki MT

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Mcm8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム