Vps18 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps18
Entrez Gene ID	296083
Full Name	VPS18 CORVET/HOPS core subunit
General protein information	Preferred Names VPS18 CORVET/HOPS core subunit Names vacuolar protein sorting-associated protein 18 homolog vacuolar protein sorting 18 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106499.2	NP_001099969.1	vacuolar protein sorting-associated protein 18 homolog

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000983	XP_560205.4
Magnaporthe oryzae (rice blast fungus)	MGG_06325	XP_003717259.1
Schizosaccharomyces pombe (fission yeast)	pep3	NP_588498.1
Arabidopsis thaliana (thale cress)	AT1G12470	NP_172709.2
Homo sapiens (human)	VPS18	NP_065908.1
Mus musculus (house mouse)	Vps18	NP_758473.3
Rattus norvegicus (Norway rat)	Vps18	NP_001099969.1
Gallus gallus (chicken)	VPS18	NP_001186584.1
Drosophila melanogaster (fruit fly)	dor	NP_477286.2
Xenopus tropicalis (tropical clawed frog)	vps18	NP_001121454.1
	VPS18	XP_001099146.1
Canis lupus familiaris (dog)	VPS18	XP_544627.3
Oryza sativa (rice)	Os08g0178100	NP_001061126.1
Pan troglodytes (chimpanzee)	VPS18	XP_523187.2
Bos taurus (cattle)	VPS18	NP_001093850.1
Danio rerio (zebrafish)	vps18	NP_775352.2
Neurospora crassa	NCU06183	XP_962995.2

Gene Ontology
Bibliography

Related articles in PubMed

Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome Fusion with Endosomes and Autophagosomes.
Wartosch L, G?nesdogan U, Graham SC, Luzio JP
Traffic (Copenhagen, Denmark)16(7)727-42(2015 Jul)

Mammalian CORVET is required for fusion and conversion of distinct early endosome subpopulations.
Perini ED, Schaefer R, St?ter M, Kalaidzidis Y, Zerial M
Traffic (Copenhagen, Denmark)15(12)1366-89(2014 Dec)

UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs.
Pirooz SD, He S, Zhang T, Zhang X, Zhao Z, Oh S, O'Connell D, Khalilzadeh P, Amini-Bavil-Olyaee S, Farzan M, Liang C
Proceedings of the National Academy of Sciences of the United States of America111(7)2716-21(2014 Feb)

Structural basis of Vps33A recruitment to the human HOPS complex by Vps16.
Graham SC, Wartosch L, Gray SR, Scourfield EJ, Deane JE, Luzio JP, Owen DJ
Proceedings of the National Academy of Sciences of the United States of America110(33)13345-50(2013 Aug)

Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.
Zlatic SA, Tornieri K, L'Hernault SW, Faundez V
Molecular biology of the cell22(10)1699-715(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vps18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01482	NM_001106499.2 Latest version!	Rattus norvegicus VPS18 core subunit of CORVET and HOPS complexes (Vps18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps18 ( NM_001106499.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099969.1 Rattus norvegicus VPS18 core subunit of CORVET and HOPS complexes (Vps18), mRNA.

Price & Availability↑

CloneID	ORa01482
Clone ID Related Accession (Same CDS sequence)	NM_001106499.2
Accession Version	NM_001106499.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Rattus norvegicus(Norway rat)
Product	vacuolar protein sorting-associated protein 18 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC169083.1. On Sep 4, 2008 this sequence version replaced NM_001106499.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC169083.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCCA TACTGGATGA GTATGAGGAC TCATTGTCCC GCTCGGCCGT CTTGCAGACT 
GGTTGCCCCA GCGTGGGCAT CCCCCATTCT GGGTATGTAA ATGCCCATCT GGAGAAGGAG 
GTGCCTATCT TCACAAAGCA GCGGATTGAC TTCACCCCCT CCGAGCGAAT CACCAGCCTT 
GTTGTTTCCT GCAGTCAGCT CTGTATGAGC CTGGGCAAGG ATACGCTGCT CCGCATTGAC 
TTGGGCAAGG CAAGCGAACC CAACCGTGTG GAGCTGGGCC GCAAGGATGA TGCCAAAGTC 
CACAAGATGT TCCTGGACCA CACTGGCTCT CATCTGCTGG TTGCTCTGAG TAGCACCGAG 
GTCCTGTACA TGAACCGCAA TGGACAGAAG GCCCGGCCGC TGGCTCGCTG GAAGGGGCAG 
CTGGTGGAGA GTGTGGGTTG GAACAAAGCG CTGGGCAGTG AGAGCAGCAC CGGCCCCATC 
CTGGTTGGCA CAGCTCAAGG ACAGATCTTT GAAGCAGAGC TCTCAGCTAG CGAAGGCGGC 
CTCTTTGGCC CTGCCCCTGA TCTCTACTTC CGTCCACTGT ATGTCTTAAA TGAAGAAGGG 
GGTCCAGCAC CTGTGTGCTC CCTTGAGGCG GAGCGTGGCC CTGACGGACG GGGCTTTGTC 
ATTGCTACCA CTCGGCAGCG CCTCTTCCAG TTCATAGGCC GAGCAGTAGA AGATGCTGAA 
GCCCAAGGCT TCTCGGGCCT CTTTGCAGCC TATACAGACC ACCCACCGCC ATTCCGTGAG 
TTTCCTGGCA ACTTGGGGTA TAGTGAGTTG GCTTTCTATA CCCCTAAGTT ACGCTCTGCA 
CCCCGTGCCT TTGCCTGGAT GATGGGAGAT GGTGTGCTGT ATGGCTCACT GGACTGCGGG 
CGCCCTGACT CCCTGCTGAG CGAGGAGAAA GTGTGGGAAT ACCCAGCAGG GATTGGTCCT 
GGGGCCAATC CACCCTTAGC CATCGTCTTG ACCCAGTTCC ATTTCCTACT GCTGCTGGCG 
GACCGGGTGG AGGCTGTGTG TACTCTAACA GGGCAGGTGG TGCTACGGGA TCACTTCCTG 
GAGAAGTTTG GACCACTGAG GCACATGGTG AAGGACTCGT CCACGGGTCA CCTCTGGGCC 
TACACTGAGC GAGCAGTCTT CCGATACCAC GTGCAACGTG AGGCTCGGGA TGTCTGGCGC 
ACCTACCTGG ACATGAACCG CTTTGACCTG GCCAAAGAGT ATTGTCGCGA GCGGCCAGAT 
TGCCTAGACA CAGTCCTGGC CCGAGAGGCT GATTTCTGCT TTCGTCAGCA TCGCTACCTG 
GAGAGCGCCC GCTGCTATGC TCTGACCCAG AGCTATTTTG AGGAGATTGC CCTCAAGTTT 
TTGGAGGCCC GGCAAGAAGA GGCACTGGCT GAGTTTCTGC AGCGGAAACT GGCCGGCTTG 
AAGCCAACTG AGCGTACTCA GGCCACACTA CTGACCACTT GGCTGACAGA GCTCTACCTG 
AGCCGGCTTG GTGCTCTGCA GGGTGACCCA GACGCCCTGA ATCTCTACCG GGATACACGG 
GAGTGCTTCC GTACTTTTCT CAGTAGCCCT CGGCACAAAG AGTGGCTCTT TGCCAGCCGG 
GCCTCTATCC ATGAGCTGCT TGCCAGTCAC GGGGACACAG AGCATATGGT GTATTTTGCA 
GTGATCATGC AGGACTATGA ACGGGTGGTC ACATACCACT GCCAGCATGA GGCCTATGAG 
GAGGCCCTGG CTGTGCTAGC CCGCCACCGA GACCCCCAGC TCTTCTACAA ATTCTCGCCT 
GTCCTCATCC GACATATCCC CCGCCAGCTG GTGGACGCCT GGATTGAGAT GGGCAGCCGG 
CTGGATGCCC GGCAGCTCAT TCCTGCCCTG GTGAACTATA GCCAGGGGGG CGACGCTCAG 
CAGGTGAGCC AAGCCATCCG CTATATGGAA TTCTGTGTGA ATGTGCTTGG CGAGACTGAG 
CAGGCCATTC ACAACTACCT GCTGTCACTG TATGCCCGTG GCCAGCCAGC CTCACTTCTG 
GCCTACCTGG AACAAGCTGG GGCCAGCCCG CACCGTGTAC ACTATGATCT CAAATATGCA 
CTTCGACTTT GTGCTGAGCA CGGCCACCAC CGCGCCTGCG TCCATGTCTA CAAGGTGTTG 
GAGCTGTATG AAGAGGCTGT GGACCTGGCC CTGCAGGTGG ATGTGGACCT GGCCAAGCAG 
TGTGCAGACC TGCCAGAGGA GGATGAGGAA CTTCGGAAGA AACTATGGCT CAAGATCGCT 
CGGCATGTGG TACAGGAGGA GGAGGACGTA CAGACAGCCA TGGCCTGCTT GGCCAGCTGC 
CCCTTGCTCA AGATTGAGGA TGTGTTGCCC TTCTTTCCTG ACTTTGTCAC AATCGACCAC 
TTTAAGGAGG CAATCTGTAG TTCACTTAAG GCCTACAACC ACCATATCCA GGAGCTACAG 
CGCGAGATGG AGGAAGCCAC AGCCAGCGCC CAGCGTATCC GACGAGACTT GCAGGAGCTT 
CGAGGGCGCT ATGGCACCGT GGAGCCCCAG GACAAATGCT CCACCTGTGA CTTCCCTTTG 
CTCAACCGCC CCTTTTACCT TTTTCTCTGT GGCCACATGT TCCACGCTGA CTGTCTGCTG 
CAGGCCGTGA GGCCTGGCCT TCCTACCTAC AAACAGGCCA GGCTTGAGGA GTTGCAGCGC 
AAGCTCGGGA CTGCGCCTCC TCCAACCAAA GGCTCTGTGA AGGCCAAGGA GGCGGAAGCT 
GGGGCTGCAG CTGTGGGGCC CAGCCGGGAA CAGCTCAAGG CTGACCTCGA TGAGCTGGTG 
GCTGCTGAGT GTGTGTACTG TGGGGAGCTG ATGATTCGCT CCATTGACCG GCCCTTCATC 
GACCCCCAGC ACTATGAGGA GGAACACCTC AGCTGGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099969.1
CDS	257..3178
Translation	MASILDEYEDSLSRSAVLQTGCPSVGIPHSGYVNAHLEKEVPIFTKQRIDFTPSERITSLVVSCSQLCMSLGKDTLLRIDLGKASEPNRVELGRKDDAKVHKMFLDHTGSHLLVALSSTEVLYMNRNGQKARPLARWKGQLVESVGWNKALGSESSTGPILVGTAQGQIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRGFVIATTRQRLFQFIGRAVEDAEAQGFSGLFAAYTDHPPPFREFPGNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGSLDCGRPDSLLSEEKVWEYPAGIGPGANPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLRHMVKDSSTGHLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQHRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLAGLKPTERTQATLLTTWLTELYLSRLGALQGDPDALNLYRDTRECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVTYHCQHEAYEEALAVLARHRDPQLFYKFSPVLIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGDAQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGQPASLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCSTCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPTYKQARLEELQRKLGTAPPPTKGSVKAKEAEAGAAAVGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQHYEEEHLSWL

Target ORF information:

RefSeq Version	NM_001106499.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus VPS18 core subunit of CORVET and HOPS complexes (Vps18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106499.2

ORF Insert Sequence:

ATGGCGTCCA TACTGGATGA GTATGAGGAC TCATTGTCCC GCTCGGCCGT CTTGCAGACT 
GGTTGCCCCA GCGTGGGCAT CCCCCATTCT GGGTATGTAA ATGCCCATCT GGAGAAGGAG 
GTGCCTATCT TCACAAAGCA GCGGATTGAC TTCACCCCCT CCGAGCGAAT CACCAGCCTT 
GTTGTTTCCT GCAGTCAGCT CTGTATGAGC CTGGGCAAGG ATACGCTGCT CCGCATTGAC 
TTGGGCAAGG CAAGCGAACC CAACCGTGTG GAGCTGGGCC GCAAGGATGA TGCCAAAGTC 
CACAAGATGT TCCTGGACCA CACTGGCTCT CATCTGCTGG TTGCTCTGAG TAGCACCGAG 
GTCCTGTACA TGAACCGCAA TGGACAGAAG GCCCGGCCGC TGGCTCGCTG GAAGGGGCAG 
CTGGTGGAGA GTGTGGGTTG GAACAAAGCG CTGGGCAGTG AGAGCAGCAC CGGCCCCATC 
CTGGTTGGCA CAGCTCAAGG ACAGATCTTT GAAGCAGAGC TCTCAGCTAG CGAAGGCGGC 
CTCTTTGGCC CTGCCCCTGA TCTCTACTTC CGTCCACTGT ATGTCTTAAA TGAAGAAGGG 
GGTCCAGCAC CTGTGTGCTC CCTTGAGGCG GAGCGTGGCC CTGACGGACG GGGCTTTGTC 
ATTGCTACCA CTCGGCAGCG CCTCTTCCAG TTCATAGGCC GAGCAGTAGA AGATGCTGAA 
GCCCAAGGCT TCTCGGGCCT CTTTGCAGCC TATACAGACC ACCCACCGCC ATTCCGTGAG 
TTTCCTGGCA ACTTGGGGTA TAGTGAGTTG GCTTTCTATA CCCCTAAGTT ACGCTCTGCA 
CCCCGTGCCT TTGCCTGGAT GATGGGAGAT GGTGTGCTGT ATGGCTCACT GGACTGCGGG 
CGCCCTGACT CCCTGCTGAG CGAGGAGAAA GTGTGGGAAT ACCCAGCAGG GATTGGTCCT 
GGGGCCAATC CACCCTTAGC CATCGTCTTG ACCCAGTTCC ATTTCCTACT GCTGCTGGCG 
GACCGGGTGG AGGCTGTGTG TACTCTAACA GGGCAGGTGG TGCTACGGGA TCACTTCCTG 
GAGAAGTTTG GACCACTGAG GCACATGGTG AAGGACTCGT CCACGGGTCA CCTCTGGGCC 
TACACTGAGC GAGCAGTCTT CCGATACCAC GTGCAACGTG AGGCTCGGGA TGTCTGGCGC 
ACCTACCTGG ACATGAACCG CTTTGACCTG GCCAAAGAGT ATTGTCGCGA GCGGCCAGAT 
TGCCTAGACA CAGTCCTGGC CCGAGAGGCT GATTTCTGCT TTCGTCAGCA TCGCTACCTG 
GAGAGCGCCC GCTGCTATGC TCTGACCCAG AGCTATTTTG AGGAGATTGC CCTCAAGTTT 
TTGGAGGCCC GGCAAGAAGA GGCACTGGCT GAGTTTCTGC AGCGGAAACT GGCCGGCTTG 
AAGCCAACTG AGCGTACTCA GGCCACACTA CTGACCACTT GGCTGACAGA GCTCTACCTG 
AGCCGGCTTG GTGCTCTGCA GGGTGACCCA GACGCCCTGA ATCTCTACCG GGATACACGG 
GAGTGCTTCC GTACTTTTCT CAGTAGCCCT CGGCACAAAG AGTGGCTCTT TGCCAGCCGG 
GCCTCTATCC ATGAGCTGCT TGCCAGTCAC GGGGACACAG AGCATATGGT GTATTTTGCA 
GTGATCATGC AGGACTATGA ACGGGTGGTC ACATACCACT GCCAGCATGA GGCCTATGAG 
GAGGCCCTGG CTGTGCTAGC CCGCCACCGA GACCCCCAGC TCTTCTACAA ATTCTCGCCT 
GTCCTCATCC GACATATCCC CCGCCAGCTG GTGGACGCCT GGATTGAGAT GGGCAGCCGG 
CTGGATGCCC GGCAGCTCAT TCCTGCCCTG GTGAACTATA GCCAGGGGGG CGACGCTCAG 
CAGGTGAGCC AAGCCATCCG CTATATGGAA TTCTGTGTGA ATGTGCTTGG CGAGACTGAG 
CAGGCCATTC ACAACTACCT GCTGTCACTG TATGCCCGTG GCCAGCCAGC CTCACTTCTG 
GCCTACCTGG AACAAGCTGG GGCCAGCCCG CACCGTGTAC ACTATGATCT CAAATATGCA 
CTTCGACTTT GTGCTGAGCA CGGCCACCAC CGCGCCTGCG TCCATGTCTA CAAGGTGTTG 
GAGCTGTATG AAGAGGCTGT GGACCTGGCC CTGCAGGTGG ATGTGGACCT GGCCAAGCAG 
TGTGCAGACC TGCCAGAGGA GGATGAGGAA CTTCGGAAGA AACTATGGCT CAAGATCGCT 
CGGCATGTGG TACAGGAGGA GGAGGACGTA CAGACAGCCA TGGCCTGCTT GGCCAGCTGC 
CCCTTGCTCA AGATTGAGGA TGTGTTGCCC TTCTTTCCTG ACTTTGTCAC AATCGACCAC 
TTTAAGGAGG CAATCTGTAG TTCACTTAAG GCCTACAACC ACCATATCCA GGAGCTACAG 
CGCGAGATGG AGGAAGCCAC AGCCAGCGCC CAGCGTATCC GACGAGACTT GCAGGAGCTT 
CGAGGGCGCT ATGGCACCGT GGAGCCCCAG GACAAATGCT CCACCTGTGA CTTCCCTTTG 
CTCAACCGCC CCTTTTACCT TTTTCTCTGT GGCCACATGT TCCACGCTGA CTGTCTGCTG 
CAGGCCGTGA GGCCTGGCCT TCCTACCTAC AAACAGGCCA GGCTTGAGGA GTTGCAGCGC 
AAGCTCGGGA CTGCGCCTCC TCCAACCAAA GGCTCTGTGA AGGCCAAGGA GGCGGAAGCT 
GGGGCTGCAG CTGTGGGGCC CAGCCGGGAA CAGCTCAAGG CTGACCTCGA TGAGCTGGTG 
GCTGCTGAGT GTGTGTACTG TGGGGAGCTG ATGATTCGCT CCATTGACCG GCCCTTCATC 
GACCCCCAGC ACTATGAGGA GGAACACCTC AGCTGGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome Fusion with Endosomes and Autophagosomes.
Traffic (Copenhagen, Denmark)16(7)727-42(2015 Jul)
Wartosch L,G?nesdogan U,Graham SC,Luzio JP

Mammalian CORVET is required for fusion and conversion of distinct early endosome subpopulations.
Traffic (Copenhagen, Denmark)15(12)1366-89(2014 Dec)
Perini ED,Schaefer R,St?ter M,Kalaidzidis Y,Zerial M

UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs.
Proceedings of the National Academy of Sciences of the United States of America111(7)2716-21(2014 Feb)
Pirooz SD,He S,Zhang T,Zhang X,Zhao Z,Oh S,O'Connell D,Khalilzadeh P,Amini-Bavil-Olyaee S,Farzan M,Liang C

Structural basis of Vps33A recruitment to the human HOPS complex by Vps16.
Proceedings of the National Academy of Sciences of the United States of America110(33)13345-50(2013 Aug)
Graham SC,Wartosch L,Gray SR,Scourfield EJ,Deane JE,Luzio JP,Owen DJ

Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.
Molecular biology of the cell22(10)1699-715(2011 May)
Zlatic SA,Tornieri K,L'Hernault SW,Faundez V

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Vps18 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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