Pdha1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdha1
Entrez Gene ID	29554
Full Name	pyruvate dehydrogenase E1 alpha 1 subunit
General protein information	Preferred Names pyruvate dehydrogenase E1 alpha 1 subunit Names pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial PDHE1-A type I pyruvate dehydrogenase (lipoamide) alpha 1 pyruvate dehydrogenase E1 alpha 1 pyruvate dehydrogenase E1alpha subunit pyruvate dehydrogenase alpha 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq14
Summary	catalyzes the interconversion of pyruvate and lipoamide to S-acetyldihydrolipoamide and CO2 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004072.2	NP_001004072.2	pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PDHA1	NP_001012562.1
Neurospora crassa	NCU06482	XP_957122.1
Oryza sativa (rice)	Os02g0739600	NP_001048068.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004773	XP_001689086.1
Xenopus tropicalis (tropical clawed frog)	pdha1	NP_001017072.1
Canis lupus familiaris (dog)	PDHA1	XP_003435519.1
Bos taurus (cattle)	PDHA1	NP_001094516.1
Rattus norvegicus (Norway rat)	Pdha1	NP_001004072.2
Danio rerio (zebrafish)	pdha1a	NP_998558.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004786	XP_318026.4
Caenorhabditis elegans (roundworm)	pdha-1	NP_871953.1
Saccharomyces cerevisiae (baker's yeast)	PDA1	NP_011105.4
Schizosaccharomyces pombe (fission yeast)	pda1	NP_594892.1
Magnaporthe oryzae (rice blast fungus)	MGG_06371	XP_003717203.1
Oryza sativa (rice)	Os06g0246500	NP_001057281.1
Homo sapiens (human)	PDHA1	NP_001166926.1
	LOC719277	XP_001113273.2
Mus musculus (house mouse)	Pdha1	NP_032836.1
Danio rerio (zebrafish)	pdha1b	NP_001002399.1
Eremothecium gossypii	AGOS_AGR103W	NP_986769.1
Arabidopsis thaliana (thale cress)	IAR4	NP_173828.1
Arabidopsis thaliana (thale cress)	E1 ALPHA	NP_176198.1
Pan troglodytes (chimpanzee)	PDHA1	NP_001104283.1
Kluyveromyces lactis	KLLA0F12001g	XP_455624.1

Gene Ontology
Bibliography

Related articles in PubMed

Pyruvate dehydrogenase has a major role in mast cell function, and its activity is regulated by mitochondrial microphthalmia transcription factor.
Sharkia I, Hadad Erlich T, Landolina N, Assayag M, Motzik A, Rachmin I, Kay G, Porat Z, Tshori S, Berkman N, Levi-Schaffer F, Razin E
The Journal of allergy and clinical immunology140(1)204-214.e8(2017 Jul)

Specific inhibition by synthetic analogs of pyruvate reveals that the pyruvate dehydrogenase reaction is essential for metabolism and viability of glioblastoma cells.
Bunik VI, Artiukhov A, Kazantsev A, Goncalves R, Daloso D, Oppermann H, Kulakovskaya E, Lukashev N, Fernie A, Brand M, Gaunitz F
Oncotarget6(37)40036-52(2015 Nov)

Pyruvate dehydrogenase E1? phosphorylation is induced by glucose but does not control metabolism-secretion coupling in INS-1E clonal ?-cells.
Akhmedov D, De Marchi U, Wollheim CB, Wiederkehr A
Biochimica et biophysica acta1823(10)1815-24(2012 Oct)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, Le?n IR, Bak S, Mogensen M, Wrzesinski K, H?jlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The association of mitochondrial microphthalmia-associated transcription factor (MITF) with pyruvate dehydrogenase (PDH) emerges as an important regulator of mast cell function. Our findings indicate that PDH could arise as a new target for the manipulation of allergic diseases.
Title: Pyruvate dehydrogenase has a major role in mast cell function, and its activity is regulated by mitochondrial microphthalmia transcription factor.

Data indicate that catalytic transformation of pyruvate by pyruvate dehydrogenase is essential for the metabolism and viability of glioblastoma cell lines.
Title: Specific inhibition by synthetic analogs of pyruvate reveals that the pyruvate dehydrogenase reaction is essential for metabolism and viability of glioblastoma cells.

Although glucose stimulates E1alpha phosphorylation and therefore inhibits PDH activity, this control mechanism by itself does not alter metabolism-secretion coupling in INS-1E clonal beta-cells.
Title: Pyruvate dehydrogenase E1? phosphorylation is induced by glucose but does not control metabolism-secretion coupling in INS-1E clonal ?-cells.

traumatic brain injury induced change in PDHE1alpha1 expression and phosphorylation could alter brain pyruvate dehydrogenase activity and glucose metabolism.
Title: Traumatic brain injury-induced expression and phosphorylation of pyruvate dehydrogenase: a mechanism of dysregulated glucose metabolism.

The following Pdha1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdha1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12513	NM_001004072.2 Latest version!	Rattus norvegicus pyruvate dehydrogenase E1 subunit alpha 1 (Pdha1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdha1 ( NM_001004072.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001004072.2 Rattus norvegicus pyruvate dehydrogenase E1 subunit alpha 1 (Pdha1), mRNA.

Price & Availability↑

CloneID	ORa12513
Clone ID Related Accession (Same CDS sequence)	NM_001004072.2
Accession Version	NM_001004072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-10
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC098897.1. On Feb 3, 2007 this sequence version replaced NM_001004072.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098897.1, BC079369.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAGGAAGA TGCTTGCCGC TGTGTCCCGC GTGTTGGCAG GCGCTGCGCA GAAGCCGGCA 
AGCAGAGTGC TGGTAGCTTC CCGTAATTTC GCAAATGATG CTACATTTGA GATTAAGAAA 
TGTGACCTTC ATCGTCTAGA AGAGGGCCCT CCAGTCACCA CAGTGCTCAC CAGAGAGGAT 
GGGCTCAAGT ACTACAGGAT GATGCAGACC GTGCGCCGGA TGGAGCTCAA GGCGGATCAG 
CTGTATAAGC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCCTGC 
TGTGTGGGCC TGGAGGCTGG CATAAACCCC ACGGACCATC TCATCACTGC CTATCGAGCC 
CATGGCTTCA CCTTCACTCG GGGCCTGCCT GTCCGAGCAA TTCTTGCAGA ATTAACAGGA 
CGAAGAGGAG GCTGTGCTAA GGGGAAAGGC GGATCAATGC ACATGTACGC CAAGAACTTC 
TATGGCGGCA ACGGTATCGT TGGAGCTCAG GTGCCCCTGG GTGCAGGAAT TGCTCTGGCC 
TGCAAGTATA ATGGCAAAGA TGAGGTCTGT TTGACATTAT ATGGTGATGG TGCTGCTAAC 
CAGGGTCAGA TATTTGAAGC TTACAATATG GCAGCATTGT GGAAATTACC TTGTATTTTC 
ATCTGTGAGA ATAACCGCTA TGGCATGGGC ACATCTGTTG AGAGAGCAGC AGCCAGCACG 
GATTACTACA AAAGAGGAGA TTTCATTCCT GGACTGAGGG TAGATGGAAT GGATATCTTG 
TGTGTCCGAG AGGCAACAAA GTTTGCAGCT GCCTACTGCA GGTCTGGTAA GGGGCCTATC 
CTGATGGAGC TACAGACTTA CCGCTACCAT GGACATAGTA TGAGTGACCC TGGAGTCAGT 
TATCGCACTC GAGAAGAAAT CCAGGAAGTA AGAAGTAAGA GTGACCCTAT TATGCTTCTC 
AAGGATAGAA TGGTGAACAG CAATCTTGCA AGTGTTGAAG AATTAAAGGA GATTGATGTG 
GAAGTGAGGA AAGAAATCGA GGATGCTGCC CAGTTTGCCA CGGCTGATCC GGAGCCGCCA 
TTGGAGGAGC TAGGCTATCA TATCTACAGC AGTGACCCAC CCTTCGAAGT GCGTGGTGCC 
AACCAGTGGA TCAAGTTTAA GTCAGTCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004072.2
CDS	39..1211
Translation	MRKMLAAVSRVLAGAAQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLPVRAILAELTGRRGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATKFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

Target ORF information:

RefSeq Version	NM_001004072.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate dehydrogenase E1 subunit alpha 1 (Pdha1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004072.2

ORF Insert Sequence:

ATGAGGAAGA TGCTTGCCGC TGTGTCCCGC GTGTTGGCAG GCGCTGCGCA GAAGCCGGCA 
AGCAGAGTGC TGGTAGCTTC CCGTAATTTC GCAAATGATG CTACATTTGA GATTAAGAAA 
TGTGACCTTC ATCGTCTAGA AGAGGGCCCT CCAGTCACCA CAGTGCTCAC CAGAGAGGAT 
GGGCTCAAGT ACTACAGGAT GATGCAGACC GTGCGCCGGA TGGAGCTCAA GGCGGATCAG 
CTGTATAAGC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCCTGC 
TGTGTGGGCC TGGAGGCTGG CATAAACCCC ACGGACCATC TCATCACTGC CTATCGAGCC 
CATGGCTTCA CCTTCACTCG GGGCCTGCCT GTCCGAGCAA TTCTTGCAGA ATTAACAGGA 
CGAAGAGGAG GCTGTGCTAA GGGGAAAGGC GGATCAATGC ACATGTACGC CAAGAACTTC 
TATGGCGGCA ACGGTATCGT TGGAGCTCAG GTGCCCCTGG GTGCAGGAAT TGCTCTGGCC 
TGCAAGTATA ATGGCAAAGA TGAGGTCTGT TTGACATTAT ATGGTGATGG TGCTGCTAAC 
CAGGGTCAGA TATTTGAAGC TTACAATATG GCAGCATTGT GGAAATTACC TTGTATTTTC 
ATCTGTGAGA ATAACCGCTA TGGCATGGGC ACATCTGTTG AGAGAGCAGC AGCCAGCACG 
GATTACTACA AAAGAGGAGA TTTCATTCCT GGACTGAGGG TAGATGGAAT GGATATCTTG 
TGTGTCCGAG AGGCAACAAA GTTTGCAGCT GCCTACTGCA GGTCTGGTAA GGGGCCTATC 
CTGATGGAGC TACAGACTTA CCGCTACCAT GGACATAGTA TGAGTGACCC TGGAGTCAGT 
TATCGCACTC GAGAAGAAAT CCAGGAAGTA AGAAGTAAGA GTGACCCTAT TATGCTTCTC 
AAGGATAGAA TGGTGAACAG CAATCTTGCA AGTGTTGAAG AATTAAAGGA GATTGATGTG 
GAAGTGAGGA AAGAAATCGA GGATGCTGCC CAGTTTGCCA CGGCTGATCC GGAGCCGCCA 
TTGGAGGAGC TAGGCTATCA TATCTACAGC AGTGACCCAC CCTTCGAAGT GCGTGGTGCC 
AACCAGTGGA TCAAGTTTAA GTCAGTCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pyruvate dehydrogenase has a major role in mast cell function, and its activity is regulated by mitochondrial microphthalmia transcription factor.
The Journal of allergy and clinical immunology140(1)204-214.e8(2017 Jul)
Sharkia I,Hadad Erlich T,Landolina N,Assayag M,Motzik A,Rachmin I,Kay G,Porat Z,Tshori S,Berkman N,Levi-Schaffer F,Razin E

Specific inhibition by synthetic analogs of pyruvate reveals that the pyruvate dehydrogenase reaction is essential for metabolism and viability of glioblastoma cells.
Oncotarget6(37)40036-52(2015 Nov)
Bunik VI,Artiukhov A,Kazantsev A,Goncalves R,Daloso D,Oppermann H,Kulakovskaya E,Lukashev N,Fernie A,Brand M,Gaunitz F

Pyruvate dehydrogenase E1? phosphorylation is induced by glucose but does not control metabolism-secretion coupling in INS-1E clonal ?-cells.
Biochimica et biophysica acta1823(10)1815-24(2012 Oct)
Akhmedov D,De Marchi U,Wollheim CB,Wiederkehr A

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)
Zhao X,Le?n IR,Bak S,Mogensen M,Wrzesinski K,H?jlund K,Jensen ON

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Pdha1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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