Acad9 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acad9
Entrez Gene ID	294973
Full Name	acyl-CoA dehydrogenase family, member 9
Synonyms	Nyggf2
General protein information	Preferred Names acyl-CoA dehydrogenase family, member 9 Names acyl-CoA dehydrogenase family member 9, mitochondrial acyl-Coenzyme A dehydrogenase family, member 9
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q25

mRNA and Protein(s)

mRNA	Protein	Name
NM_181768.2	NP_861433.2	complex I assembly factor ACAD9, mitochondrial
XM_006232269.1	XP_006232331.1	acyl-CoA dehydrogenase family member 9, mitochondrial isoform X1
XM_006232270.3	XP_006232332.1	acyl-CoA dehydrogenase family member 9, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ACAD9	NP_001071544.1
Danio rerio (zebrafish)	acad9	NP_001038772.1
Xenopus tropicalis (tropical clawed frog)	acad9	NP_001015788.1
Homo sapiens (human)	ACAD9	NP_054768.2
Pan troglodytes (chimpanzee)	ACAD9	XP_001140269.1
Canis lupus familiaris (dog)	ACAD9	XP_533725.2
	ACAD9	XP_001096856.1
Mus musculus (house mouse)	Acad9	NP_766266.3
Rattus norvegicus (Norway rat)	Acad9	NP_861433.2
Gallus gallus (chicken)	ACAD9	NP_001006136.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of the Catalytic Residue of Rat Acyl-CoA Dehydrogenase 9 by Site-Directed Mutagenesis.
Zeng J, Deng S, Wang Y
Applied biochemistry and biotechnology182(3)1198-1207(2017 Jul)

Identification and characterization of new long chain acyl-CoA dehydrogenases.
He M, Pei Z, Mohsen AW, Watkins P, Murdoch G, Van Veldhoven PP, Ensenauer R, Vockley J
Molecular genetics and metabolism102(4)418-29(2011 Apr)

Acyl-CoA dehydrogenase 9 is required for the biogenesis of oxidative phosphorylation complex I.
Nouws J, Nijtmans L, Houten SM, van den Brand M, Huynen M, Venselaar H, Hoefs S, Gloerich J, Kronick J, Hutchin T, Willems P, Rodenburg R, Wanders R, van den Heuvel L, Smeitink J, Vogel RO
Cell metabolism12(3)283-94(2010 Sep)

Carnitine palmitoyltransferase I and Acyl-CoA dehydrogenase 9 in retina: insights of retinopathy in mitochondrial trifunctional protein defects.
Roomets E, Kivel? T, Tyni T
Investigative ophthalmology & visual science49(4)1660-4(2008 Apr)

Human acyl-CoA dehydrogenase-9 plays a novel role in the mitochondrial beta-oxidation of unsaturated fatty acids.
Ensenauer R, He M, Willard JM, Goetzman ES, Corydon TJ, Vandahl BB, Mohsen AW, Isaya G, Vockley J
The Journal of biological chemistry280(37)32309-16(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggested that Glu389 could function as the catalytic base that abstracted the alpha-proton of the acyl-CoA substrate in a proposed catalytic mechanism.
Title: Identification of the Catalytic Residue of Rat Acyl-CoA Dehydrogenase 9 by Site-Directed Mutagenesis.

Accumulation of 3-hydroxylated intermediates of long-chain fatty acids may contribute to the pathogenesis of retinopathy in MTP deficiencies.
Title: Carnitine palmitoyltransferase I and Acyl-CoA dehydrogenase 9 in retina: insights of retinopathy in mitochondrial trifunctional protein defects.

The following Acad9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acad9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04470	NM_181768.2 Latest version!	Rattus norvegicus acyl-CoA dehydrogenase family, member 9 (Acad9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa04470	XM_006232269.1 Latest version!	Rattus norvegicus acyl-CoA dehydrogenase family, member 9 (Acad9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa24625	XM_006232270.3 Latest version!	Rattus norvegicus acyl-CoA dehydrogenase family, member 9 (Acad9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acad9 ( NM_181768.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_861433.2 Rattus norvegicus acyl-CoA dehydrogenase family, member 9 (Acad9), mRNA.

Price & Availability↑

CloneID	ORa04470
Clone ID Related Accession (Same CDS sequence)	NM_181768.2 , XM_006232269.1
Accession Version	NM_181768.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	complex I assembly factor ACAD9, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC162016.1. On or before Aug 29, 2008 this sequence version replaced XM_001061715.1, XM_001061665.1, XM_574921.2, XM_001059349.1, NM_181768.1. ##Evidence-Data-START## Transcript exon combination :: BC162016.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCTGGCT ATGTACTGTT CTCGCGTGGG GCGACTGCGG CGGCAGCTGC GGCGCGAGCT 
TCCCGCGTGC TGAGGGTCTT CACGGAGAGG CGCCGCACAT TGCACACCAG TCTGCAGAGC 
TGCTCGTTCG CCAAGGAGCT CTTCCTGGGT CACATCCAGC AGAAAGGAGT TTTCCCATTT 
CCAGAGGTTA GTCAAGAAGA GCTTAGTGAA ATCAATCAGT TTGTGGGACC GTTGGAAAAA 
TTCTTTAATG AAGAAGTGGA CTCTCGAAAA ATTGACCAGG AGGGAAAAAT CCCAGCTGAC 
ACCTTAGCGA AGTTGAAGAG CCTGGGACTT TTTGGCATTC AGGTCCCAGA AGAATATGGT 
GGCCTGGGCC TCTCCAACAC CATGTATGCC CGGCTTGGGG AGATCATCAG CATGGATGCG 
TCCATCACAG TGACCCTGGC AGCACACCAA GCTATCGGCC TCAAGGGGAT TATCCTGGTC 
GGCAATGAGG AGCAGAAGGC CAAGTATCTG CCCAAACTGT CGTCTGGGGA ACACATCGCA 
GCCTTCTGCC TAACGGAGCC AGCCAGTGGG AGTGATGCTG CCTCCATCCA GACCAGAGCT 
ACGTTAAGTG AAGATAAGAA GTACTTTGTT CTCAATGGCT CCAAGGTTTG GATCACTAAT 
GGAGGACTGG CCAATATTTT TACTGTGTTT GCAAAAACTG AGGTGGTTGA TTCTGATGGT 
TCGATAAAAG ACAAAATGAC CGCATTCATA GTAGAACGAG ACTTTGGTGG AATCACTAAT 
GGGAAACCTG AAGATAAATT AGGCATTCGA GGCTCCAACA CATGTGAAGT CCATTTTGAA 
AATACCAGAG TGCCGGTGGA GAATGTCCTT GGAGAAGTTG GGGGTGGCTT TAAGGTGGCC 
ATGAACATCC TCAACAGTGG ACGATTCAGC ATGGGCAGTG CTGTGGCCGG GATGCTCAAG 
AAACTGATTG AACAGACTGC TGAGTATGCC TGTACGAGGA AACAGTTCAA TAGGAATCTC 
AGTGAATTTG GGCTGATTCA GGAAAAGTTT GCGCTCATGG CTCAGAAGGC GTATGTAATG 
GAGAGTATGG CTTACCTCAC TTCAGGGATG CTGGACCAAC CGGGATTTCC CGACTGCTCT 
ATCGAGGCAG CCATGGTGAA GGTGTTTAGC TCTGAGGCTG CCTGGCAGTG TGTGAGCGAG 
GCTCTGCAGA TCCTTGGGGG CTCAGGCTAC ATGAAGGACT ACCCTTATGA GCGCATGCTA 
CGTGATGCCC GCATTCTCCT AATTTTTGAG GGAACCAATG AGATTCTTCG GTTGTTCATT 
GCCCTGACAG GCCTGCAGCA TGCTGGACGC ATCCTGACCT CGAGGATCAA AGAGCTCAAA 
AGTGGCAATG TGACCACAGT CATGGAGACG ATTGGTCGGA AGCTTCGGGA CTCGCTGGGC 
CGAACTGTAG ACCTGGGGCT GTCGAGCAAT ATTGCTGTTG TCCACCCCAG TCTTGGAGAC 
AGTGCCAACA AGCTGGAAGA AAATGTGCAT TACTTTGGCC GGACTGTTGA GACCCTGTTA 
CTCCGATTTG GAAAGACCAT TGTAGAGGAA CAGCTGGTGT TGAAGCGGGT AGCCAACATC 
CTCATCAACC TGTATGGCAT GACCGCTGTG CTGTCAAGGG CCAGCCGCTC CATCAGAATT 
GGGCTCAAGA ATCACGACCA TGAGATTCTG TTGGCCAACA TGTTCTGCGT GGAAGCTTAT 
TTCCAGAATC TCTTCAGCCT GTCTCAGCTG GACAAGTATG CTCCAGAGAA CCTGGATGAA 
CAGATTAAGA AGGTGTCTCA GCAGATCCTT GAGAAGCGAG CCTACATCTG TGCCCACCCT 
CTGGACAGAG CATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_861433.2
CDS	40..1917
Translation	MSGYVLFSRGATAAAAAARASRVLRVFTERRRTLHTSLQSCSFAKELFLGHIQQKGVFPFPEVSQEELSEINQFVGPLEKFFNEEVDSRKIDQEGKIPADTLAKLKSLGLFGIQVPEEYGGLGLSNTMYARLGEIISMDASITVTLAAHQAIGLKGIILVGNEEQKAKYLPKLSSGEHIAAFCLTEPASGSDAASIQTRATLSEDKKYFVLNGSKVWITNGGLANIFTVFAKTEVVDSDGSIKDKMTAFIVERDFGGITNGKPEDKLGIRGSNTCEVHFENTRVPVENVLGEVGGGFKVAMNILNSGRFSMGSAVAGMLKKLIEQTAEYACTRKQFNRNLSEFGLIQEKFALMAQKAYVMESMAYLTSGMLDQPGFPDCSIEAAMVKVFSSEAAWQCVSEALQILGGSGYMKDYPYERMLRDARILLIFEGTNEILRLFIALTGLQHAGRILTSRIKELKSGNVTTVMETIGRKLRDSLGRTVDLGLSSNIAVVHPSLGDSANKLEENVHYFGRTVETLLLRFGKTIVEEQLVLKRVANILINLYGMTAVLSRASRSIRIGLKNHDHEILLANMFCVEAYFQNLFSLSQLDKYAPENLDEQIKKVSQQILEKRAYICAHPLDRAS

Target ORF information:

RefSeq Version	NM_181768.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA dehydrogenase family, member 9 (Acad9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181768.2

ORF Insert Sequence:

ATGTCTGGCT ATGTACTGTT CTCGCGTGGG GCGACTGCGG CGGCAGCTGC GGCGCGAGCT 
TCCCGCGTGC TGAGGGTCTT CACGGAGAGG CGCCGCACAT TGCACACCAG TCTGCAGAGC 
TGCTCGTTCG CCAAGGAGCT CTTCCTGGGT CACATCCAGC AGAAAGGAGT TTTCCCATTT 
CCAGAGGTTA GTCAAGAAGA GCTTAGTGAA ATCAATCAGT TTGTGGGACC GTTGGAAAAA 
TTCTTTAATG AAGAAGTGGA CTCTCGAAAA ATTGACCAGG AGGGAAAAAT CCCAGCTGAC 
ACCTTAGCGA AGTTGAAGAG CCTGGGACTT TTTGGCATTC AGGTCCCAGA AGAATATGGT 
GGCCTGGGCC TCTCCAACAC CATGTATGCC CGGCTTGGGG AGATCATCAG CATGGATGCG 
TCCATCACAG TGACCCTGGC AGCACACCAA GCTATCGGCC TCAAGGGGAT TATCCTGGTC 
GGCAATGAGG AGCAGAAGGC CAAGTATCTG CCCAAACTGT CGTCTGGGGA ACACATCGCA 
GCCTTCTGCC TAACGGAGCC AGCCAGTGGG AGTGATGCTG CCTCCATCCA GACCAGAGCT 
ACGTTAAGTG AAGATAAGAA GTACTTTGTT CTCAATGGCT CCAAGGTTTG GATCACTAAT 
GGAGGACTGG CCAATATTTT TACTGTGTTT GCAAAAACTG AGGTGGTTGA TTCTGATGGT 
TCGATAAAAG ACAAAATGAC CGCATTCATA GTAGAACGAG ACTTTGGTGG AATCACTAAT 
GGGAAACCTG AAGATAAATT AGGCATTCGA GGCTCCAACA CATGTGAAGT CCATTTTGAA 
AATACCAGAG TGCCGGTGGA GAATGTCCTT GGAGAAGTTG GGGGTGGCTT TAAGGTGGCC 
ATGAACATCC TCAACAGTGG ACGATTCAGC ATGGGCAGTG CTGTGGCCGG GATGCTCAAG 
AAACTGATTG AACAGACTGC TGAGTATGCC TGTACGAGGA AACAGTTCAA TAGGAATCTC 
AGTGAATTTG GGCTGATTCA GGAAAAGTTT GCGCTCATGG CTCAGAAGGC GTATGTAATG 
GAGAGTATGG CTTACCTCAC TTCAGGGATG CTGGACCAAC CGGGATTTCC CGACTGCTCT 
ATCGAGGCAG CCATGGTGAA GGTGTTTAGC TCTGAGGCTG CCTGGCAGTG TGTGAGCGAG 
GCTCTGCAGA TCCTTGGGGG CTCAGGCTAC ATGAAGGACT ACCCTTATGA GCGCATGCTA 
CGTGATGCCC GCATTCTCCT AATTTTTGAG GGAACCAATG AGATTCTTCG GTTGTTCATT 
GCCCTGACAG GCCTGCAGCA TGCTGGACGC ATCCTGACCT CGAGGATCAA AGAGCTCAAA 
AGTGGCAATG TGACCACAGT CATGGAGACG ATTGGTCGGA AGCTTCGGGA CTCGCTGGGC 
CGAACTGTAG ACCTGGGGCT GTCGAGCAAT ATTGCTGTTG TCCACCCCAG TCTTGGAGAC 
AGTGCCAACA AGCTGGAAGA AAATGTGCAT TACTTTGGCC GGACTGTTGA GACCCTGTTA 
CTCCGATTTG GAAAGACCAT TGTAGAGGAA CAGCTGGTGT TGAAGCGGGT AGCCAACATC 
CTCATCAACC TGTATGGCAT GACCGCTGTG CTGTCAAGGG CCAGCCGCTC CATCAGAATT 
GGGCTCAAGA ATCACGACCA TGAGATTCTG TTGGCCAACA TGTTCTGCGT GGAAGCTTAT 
TTCCAGAATC TCTTCAGCCT GTCTCAGCTG GACAAGTATG CTCCAGAGAA CCTGGATGAA 
CAGATTAAGA AGGTGTCTCA GCAGATCCTT GAGAAGCGAG CCTACATCTG TGCCCACCCT 
CTGGACAGAG CATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acad9 ( XM_006232269.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232331.1 Rattus norvegicus acyl-CoA dehydrogenase family, member 9 (Acad9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa04470
Clone ID Related Accession (Same CDS sequence)	NM_181768.2 , XM_006232269.1
Accession Version	XM_006232269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	acyl-CoA dehydrogenase family member 9, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCT ATGTACTGTT CTCGCGTGGG GCGACTGCGG CGGCAGCTGC GGCGCGAGCT 
TCCCGCGTGC TGAGGGTCTT CACGGAGAGG CGCCGCACAT TGCACACCAG TCTGCAGAGC 
TGCTCGTTCG CCAAGGAGCT CTTCCTGGGT CACATCCAGC AGAAAGGAGT TTTCCCATTT 
CCAGAGGTTA GTCAAGAAGA GCTTAGTGAA ATCAATCAGT TTGTGGGACC GTTGGAAAAA 
TTCTTTAATG AAGAAGTGGA CTCTCGAAAA ATTGACCAGG AGGGAAAAAT CCCAGCTGAC 
ACCTTAGCGA AGTTGAAGAG CCTGGGACTT TTTGGCATTC AGGTCCCAGA AGAATATGGT 
GGCCTGGGCC TCTCCAACAC CATGTATGCC CGGCTTGGGG AGATCATCAG CATGGATGCG 
TCCATCACAG TGACCCTGGC AGCACACCAA GCTATCGGCC TCAAGGGGAT TATCCTGGTC 
GGCAATGAGG AGCAGAAGGC CAAGTATCTG CCCAAACTGT CGTCTGGGGA ACACATCGCA 
GCCTTCTGCC TAACGGAGCC AGCCAGTGGG AGTGATGCTG CCTCCATCCA GACCAGAGCT 
ACGTTAAGTG AAGATAAGAA GTACTTTGTT CTCAATGGCT CCAAGGTTTG GATCACTAAT 
GGAGGACTGG CCAATATTTT TACTGTGTTT GCAAAAACTG AGGTGGTTGA TTCTGATGGT 
TCGATAAAAG ACAAAATGAC CGCATTCATA GTAGAACGAG ACTTTGGTGG AATCACTAAT 
GGGAAACCTG AAGATAAATT AGGCATTCGA GGCTCCAACA CATGTGAAGT CCATTTTGAA 
AATACCAGAG TGCCGGTGGA GAATGTCCTT GGAGAAGTTG GGGGTGGCTT TAAGGTGGCC 
ATGAACATCC TCAACAGTGG ACGATTCAGC ATGGGCAGTG CTGTGGCCGG GATGCTCAAG 
AAACTGATTG AACAGACTGC TGAGTATGCC TGTACGAGGA AACAGTTCAA TAGGAATCTC 
AGTGAATTTG GGCTGATTCA GGAAAAGTTT GCGCTCATGG CTCAGAAGGC GTATGTAATG 
GAGAGTATGG CTTACCTCAC TTCAGGGATG CTGGACCAAC CGGGATTTCC CGACTGCTCT 
ATCGAGGCAG CCATGGTGAA GGTGTTTAGC TCTGAGGCTG CCTGGCAGTG TGTGAGCGAG 
GCTCTGCAGA TCCTTGGGGG CTCAGGCTAC ATGAAGGACT ACCCTTATGA GCGCATGCTA 
CGTGATGCCC GCATTCTCCT AATTTTTGAG GGAACCAATG AGATTCTTCG GTTGTTCATT 
GCCCTGACAG GCCTGCAGCA TGCTGGACGC ATCCTGACCT CGAGGATCAA AGAGCTCAAA 
AGTGGCAATG TGACCACAGT CATGGAGACG ATTGGTCGGA AGCTTCGGGA CTCGCTGGGC 
CGAACTGTAG ACCTGGGGCT GTCGAGCAAT ATTGCTGTTG TCCACCCCAG TCTTGGAGAC 
AGTGCCAACA AGCTGGAAGA AAATGTGCAT TACTTTGGCC GGACTGTTGA GACCCTGTTA 
CTCCGATTTG GAAAGACCAT TGTAGAGGAA CAGCTGGTGT TGAAGCGGGT AGCCAACATC 
CTCATCAACC TGTATGGCAT GACCGCTGTG CTGTCAAGGG CCAGCCGCTC CATCAGAATT 
GGGCTCAAGA ATCACGACCA TGAGATTCTG TTGGCCAACA TGTTCTGCGT GGAAGCTTAT 
TTCCAGAATC TCTTCAGCCT GTCTCAGCTG GACAAGTATG CTCCAGAGAA CCTGGATGAA 
CAGATTAAGA AGGTGTCTCA GCAGATCCTT GAGAAGCGAG CCTACATCTG TGCCCACCCT 
CTGGACAGAG CATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232331.1
CDS	120..1997
Translation	MSGYVLFSRGATAAAAAARASRVLRVFTERRRTLHTSLQSCSFAKELFLGHIQQKGVFPFPEVSQEELSEINQFVGPLEKFFNEEVDSRKIDQEGKIPADTLAKLKSLGLFGIQVPEEYGGLGLSNTMYARLGEIISMDASITVTLAAHQAIGLKGIILVGNEEQKAKYLPKLSSGEHIAAFCLTEPASGSDAASIQTRATLSEDKKYFVLNGSKVWITNGGLANIFTVFAKTEVVDSDGSIKDKMTAFIVERDFGGITNGKPEDKLGIRGSNTCEVHFENTRVPVENVLGEVGGGFKVAMNILNSGRFSMGSAVAGMLKKLIEQTAEYACTRKQFNRNLSEFGLIQEKFALMAQKAYVMESMAYLTSGMLDQPGFPDCSIEAAMVKVFSSEAAWQCVSEALQILGGSGYMKDYPYERMLRDARILLIFEGTNEILRLFIALTGLQHAGRILTSRIKELKSGNVTTVMETIGRKLRDSLGRTVDLGLSSNIAVVHPSLGDSANKLEENVHYFGRTVETLLLRFGKTIVEEQLVLKRVANILINLYGMTAVLSRASRSIRIGLKNHDHEILLANMFCVEAYFQNLFSLSQLDKYAPENLDEQIKKVSQQILEKRAYICAHPLDRAS

Target ORF information:

RefSeq Version	XM_006232269.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA dehydrogenase family, member 9 (Acad9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232269.1

ORF Insert Sequence:

ATGTCTGGCT ATGTACTGTT CTCGCGTGGG GCGACTGCGG CGGCAGCTGC GGCGCGAGCT 
TCCCGCGTGC TGAGGGTCTT CACGGAGAGG CGCCGCACAT TGCACACCAG TCTGCAGAGC 
TGCTCGTTCG CCAAGGAGCT CTTCCTGGGT CACATCCAGC AGAAAGGAGT TTTCCCATTT 
CCAGAGGTTA GTCAAGAAGA GCTTAGTGAA ATCAATCAGT TTGTGGGACC GTTGGAAAAA 
TTCTTTAATG AAGAAGTGGA CTCTCGAAAA ATTGACCAGG AGGGAAAAAT CCCAGCTGAC 
ACCTTAGCGA AGTTGAAGAG CCTGGGACTT TTTGGCATTC AGGTCCCAGA AGAATATGGT 
GGCCTGGGCC TCTCCAACAC CATGTATGCC CGGCTTGGGG AGATCATCAG CATGGATGCG 
TCCATCACAG TGACCCTGGC AGCACACCAA GCTATCGGCC TCAAGGGGAT TATCCTGGTC 
GGCAATGAGG AGCAGAAGGC CAAGTATCTG CCCAAACTGT CGTCTGGGGA ACACATCGCA 
GCCTTCTGCC TAACGGAGCC AGCCAGTGGG AGTGATGCTG CCTCCATCCA GACCAGAGCT 
ACGTTAAGTG AAGATAAGAA GTACTTTGTT CTCAATGGCT CCAAGGTTTG GATCACTAAT 
GGAGGACTGG CCAATATTTT TACTGTGTTT GCAAAAACTG AGGTGGTTGA TTCTGATGGT 
TCGATAAAAG ACAAAATGAC CGCATTCATA GTAGAACGAG ACTTTGGTGG AATCACTAAT 
GGGAAACCTG AAGATAAATT AGGCATTCGA GGCTCCAACA CATGTGAAGT CCATTTTGAA 
AATACCAGAG TGCCGGTGGA GAATGTCCTT GGAGAAGTTG GGGGTGGCTT TAAGGTGGCC 
ATGAACATCC TCAACAGTGG ACGATTCAGC ATGGGCAGTG CTGTGGCCGG GATGCTCAAG 
AAACTGATTG AACAGACTGC TGAGTATGCC TGTACGAGGA AACAGTTCAA TAGGAATCTC 
AGTGAATTTG GGCTGATTCA GGAAAAGTTT GCGCTCATGG CTCAGAAGGC GTATGTAATG 
GAGAGTATGG CTTACCTCAC TTCAGGGATG CTGGACCAAC CGGGATTTCC CGACTGCTCT 
ATCGAGGCAG CCATGGTGAA GGTGTTTAGC TCTGAGGCTG CCTGGCAGTG TGTGAGCGAG 
GCTCTGCAGA TCCTTGGGGG CTCAGGCTAC ATGAAGGACT ACCCTTATGA GCGCATGCTA 
CGTGATGCCC GCATTCTCCT AATTTTTGAG GGAACCAATG AGATTCTTCG GTTGTTCATT 
GCCCTGACAG GCCTGCAGCA TGCTGGACGC ATCCTGACCT CGAGGATCAA AGAGCTCAAA 
AGTGGCAATG TGACCACAGT CATGGAGACG ATTGGTCGGA AGCTTCGGGA CTCGCTGGGC 
CGAACTGTAG ACCTGGGGCT GTCGAGCAAT ATTGCTGTTG TCCACCCCAG TCTTGGAGAC 
AGTGCCAACA AGCTGGAAGA AAATGTGCAT TACTTTGGCC GGACTGTTGA GACCCTGTTA 
CTCCGATTTG GAAAGACCAT TGTAGAGGAA CAGCTGGTGT TGAAGCGGGT AGCCAACATC 
CTCATCAACC TGTATGGCAT GACCGCTGTG CTGTCAAGGG CCAGCCGCTC CATCAGAATT 
GGGCTCAAGA ATCACGACCA TGAGATTCTG TTGGCCAACA TGTTCTGCGT GGAAGCTTAT 
TTCCAGAATC TCTTCAGCCT GTCTCAGCTG GACAAGTATG CTCCAGAGAA CCTGGATGAA 
CAGATTAAGA AGGTGTCTCA GCAGATCCTT GAGAAGCGAG CCTACATCTG TGCCCACCCT 
CTGGACAGAG CATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acad9 ( XM_006232270.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232332.1 Rattus norvegicus acyl-CoA dehydrogenase family, member 9 (Acad9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa24625
Clone ID Related Accession (Same CDS sequence)	XM_006232270.3
Accession Version	XM_006232270.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	acyl-CoA dehydrogenase family member 9, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232270.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCT ATGTACTGTT CTCGCGTGGG GCGACTGCGG CGGCAGCTGC GGCGCGAGCT 
TCCCGCGTGC TGAGGGTCTT CACGGAGAGG CGCCGCACAT TGCACACCAG TCTGCAGAGC 
TGCTCGTTCG CCAAGGAGCT CTTCCTGGGT CACATCCAGC AGAAAGGAGT TTTCCCATTT 
CCAGAGGTTA GTCAAGAAGA GCTTAGTGAA ATCAATCAGT TTGTGGGACC GTTGGAAAAA 
TTCTTTAATG AAGAAGTGGA CTCTCGAAAA ATTGACCAGG AGGGAAAAAT CCCAGCTGAC 
ACCTTAGCGA AGTTGAAGAG CCTGGGACTT TTTGGCATTC AGGTCCCAGA AGAATATGGT 
GGCCTGGGCC TCTCCAACAC CATGTATGCC CGGCTTGGGG AGATCATCAG CATGGATGCG 
TCCATCACAG TGACCCTGGC AGCACACCAA GCTATCGGCC TCAAGGGGAT TATCCTGGTC 
GGCAATGAGG AGCAGAAGGC CAAGTATCTG CCCAAACTGT CGTCTGGGGA ACACATCGCA 
GCCTTCTGCC TAACGGAGCC AGCCAGTGGG AGTGATGCTG CCTCCATCCA GACCAGAGCT 
ACGTTAAGTG AAGATAAGAA GTACTTTGTT CTCAATGGCT CCAAGGTTTG GATCACTAAT 
GGAGGACTGG CCAATATTTT TACTGTGTTT GCAAAAACTG AGGTGGTTGA TTCTGATGGT 
TCGATAAAAG ACAAAATGAC CGCATTCATA GTAGAACGAG ACTTTGGTGG AATCACTAAT 
GGGAAACCTG AAGATAAATT AGGCATTCGA GGCTCCAACA CATGTGAAGT CCATTTTGAA 
AATACCAGAG TGCCGGTGGA GAATGTCCTT GGAGAAGTTG GGGGTGGCTT TAAGGTGGCC 
ATGAACATCC TCAACAGTGG ACGATTCAGC ATGGGCAGTG CTGTGGCCGG GATGCTCAAG 
AAACTGATTG AACAGACTGC TGAGTATGCC TGTACGAGGA AACAGTTCAA TAGGAATCTC 
AGTGAATTTG GGCTGATTCA GGAAAAGTTT GCGCTCATGG CTCAGAAGGC GTATGTAATG 
GAGAGTATGG CTTACCTCAC TTCAGGGATG CTGGACCAAC CGGGATTTCC CGACTGCTCT 
ATCGAGGCAG CCATGGTGAA GGTGTTTAGC TCTGAGGCTG CCTGGCAGTG TGTGAGCGAG 
GCTCTGCAGA TCCTTGGGGG CTCAGGCTAC ATGAAGGACT ACCCTTATGA GCGCATGCTA 
CGTGATGCCC GCATTCTCCT AATTTTTGAG GGAACCAATG AGATTCTTCG GTTGTTCATT 
GCCCTGACAG GCCTGCAGCA TGCTGGACGC ATCCTGACCT CGAGGATCAA AGAGCTCAAA 
AGTGGCAATG TGACCACAGT CATGGAGACG ATTGGTCGGA AGCTTCGGGA CTCGCTGGGC 
CGAACTGTAG ACCTGGGGCT GTCGAGCAAT ATTGCTGTTG TCCACCCCAG TCTTGGAGAC 
AGTGCCAACA AGCTGGAAGA AAATGTGCAT TACTTTGGCC GGACTGTTGA GACCCTGTTA 
CTCCGATTTG GAAAGACCAT TGTAGAGGAA CAGCTGGTGT TGAAGCGGGT AGCCAACATC 
CTCATCAACC TGTATGGCAT GACCGCTGTG CTGTCAAGGG CCAGCCGCTC CATCAGAATT 
GGGCTCAAGA ATCACGACCA TGAGGTAAGC CTTGCATGGC CCCACACAGG AAATACACAG 
AGCCACAGTG AGCTGCTGTC AAGGTCAAGC CTACTGGAAC ACTCCCTTAA TCTCACTGTG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232332.1
CDS	120..1925
Translation	MSGYVLFSRGATAAAAAARASRVLRVFTERRRTLHTSLQSCSFAKELFLGHIQQKGVFPFPEVSQEELSEINQFVGPLEKFFNEEVDSRKIDQEGKIPADTLAKLKSLGLFGIQVPEEYGGLGLSNTMYARLGEIISMDASITVTLAAHQAIGLKGIILVGNEEQKAKYLPKLSSGEHIAAFCLTEPASGSDAASIQTRATLSEDKKYFVLNGSKVWITNGGLANIFTVFAKTEVVDSDGSIKDKMTAFIVERDFGGITNGKPEDKLGIRGSNTCEVHFENTRVPVENVLGEVGGGFKVAMNILNSGRFSMGSAVAGMLKKLIEQTAEYACTRKQFNRNLSEFGLIQEKFALMAQKAYVMESMAYLTSGMLDQPGFPDCSIEAAMVKVFSSEAAWQCVSEALQILGGSGYMKDYPYERMLRDARILLIFEGTNEILRLFIALTGLQHAGRILTSRIKELKSGNVTTVMETIGRKLRDSLGRTVDLGLSSNIAVVHPSLGDSANKLEENVHYFGRTVETLLLRFGKTIVEEQLVLKRVANILINLYGMTAVLSRASRSIRIGLKNHDHEVSLAWPHTGNTQSHSELLSRSSLLEHSLNLTVL

Target ORF information:

RefSeq Version	XM_006232270.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA dehydrogenase family, member 9 (Acad9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232270.3

ORF Insert Sequence:

ATGTCTGGCT ATGTACTGTT CTCGCGTGGG GCGACTGCGG CGGCAGCTGC GGCGCGAGCT 
TCCCGCGTGC TGAGGGTCTT CACGGAGAGG CGCCGCACAT TGCACACCAG TCTGCAGAGC 
TGCTCGTTCG CCAAGGAGCT CTTCCTGGGT CACATCCAGC AGAAAGGAGT TTTCCCATTT 
CCAGAGGTTA GTCAAGAAGA GCTTAGTGAA ATCAATCAGT TTGTGGGACC GTTGGAAAAA 
TTCTTTAATG AAGAAGTGGA CTCTCGAAAA ATTGACCAGG AGGGAAAAAT CCCAGCTGAC 
ACCTTAGCGA AGTTGAAGAG CCTGGGACTT TTTGGCATTC AGGTCCCAGA AGAATATGGT 
GGCCTGGGCC TCTCCAACAC CATGTATGCC CGGCTTGGGG AGATCATCAG CATGGATGCG 
TCCATCACAG TGACCCTGGC AGCACACCAA GCTATCGGCC TCAAGGGGAT TATCCTGGTC 
GGCAATGAGG AGCAGAAGGC CAAGTATCTG CCCAAACTGT CGTCTGGGGA ACACATCGCA 
GCCTTCTGCC TAACGGAGCC AGCCAGTGGG AGTGATGCTG CCTCCATCCA GACCAGAGCT 
ACGTTAAGTG AAGATAAGAA GTACTTTGTT CTCAATGGCT CCAAGGTTTG GATCACTAAT 
GGAGGACTGG CCAATATTTT TACTGTGTTT GCAAAAACTG AGGTGGTTGA TTCTGATGGT 
TCGATAAAAG ACAAAATGAC CGCATTCATA GTAGAACGAG ACTTTGGTGG AATCACTAAT 
GGGAAACCTG AAGATAAATT AGGCATTCGA GGCTCCAACA CATGTGAAGT CCATTTTGAA 
AATACCAGAG TGCCGGTGGA GAATGTCCTT GGAGAAGTTG GGGGTGGCTT TAAGGTGGCC 
ATGAACATCC TCAACAGTGG ACGATTCAGC ATGGGCAGTG CTGTGGCCGG GATGCTCAAG 
AAACTGATTG AACAGACTGC TGAGTATGCC TGTACGAGGA AACAGTTCAA TAGGAATCTC 
AGTGAATTTG GGCTGATTCA GGAAAAGTTT GCGCTCATGG CTCAGAAGGC GTATGTAATG 
GAGAGTATGG CTTACCTCAC TTCAGGGATG CTGGACCAAC CGGGATTTCC CGACTGCTCT 
ATCGAGGCAG CCATGGTGAA GGTGTTTAGC TCTGAGGCTG CCTGGCAGTG TGTGAGCGAG 
GCTCTGCAGA TCCTTGGGGG CTCAGGCTAC ATGAAGGACT ACCCTTATGA GCGCATGCTA 
CGTGATGCCC GCATTCTCCT AATTTTTGAG GGAACCAATG AGATTCTTCG GTTGTTCATT 
GCCCTGACAG GCCTGCAGCA TGCTGGACGC ATCCTGACCT CGAGGATCAA AGAGCTCAAA 
AGTGGCAATG TGACCACAGT CATGGAGACG ATTGGTCGGA AGCTTCGGGA CTCGCTGGGC 
CGAACTGTAG ACCTGGGGCT GTCGAGCAAT ATTGCTGTTG TCCACCCCAG TCTTGGAGAC 
AGTGCCAACA AGCTGGAAGA AAATGTGCAT TACTTTGGCC GGACTGTTGA GACCCTGTTA 
CTCCGATTTG GAAAGACCAT TGTAGAGGAA CAGCTGGTGT TGAAGCGGGT AGCCAACATC 
CTCATCAACC TGTATGGCAT GACCGCTGTG CTGTCAAGGG CCAGCCGCTC CATCAGAATT 
GGGCTCAAGA ATCACGACCA TGAGGTAAGC CTTGCATGGC CCCACACAGG AAATACACAG 
AGCCACAGTG AGCTGCTGTC AAGGTCAAGC CTACTGGAAC ACTCCCTTAA TCTCACTGTG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of the Catalytic Residue of Rat Acyl-CoA Dehydrogenase 9 by Site-Directed Mutagenesis.
Applied biochemistry and biotechnology182(3)1198-1207(2017 Jul)
Zeng J,Deng S,Wang Y

Identification and characterization of new long chain acyl-CoA dehydrogenases.
Molecular genetics and metabolism102(4)418-29(2011 Apr)
He M,Pei Z,Mohsen AW,Watkins P,Murdoch G,Van Veldhoven PP,Ensenauer R,Vockley J

Acyl-CoA dehydrogenase 9 is required for the biogenesis of oxidative phosphorylation complex I.
Cell metabolism12(3)283-94(2010 Sep)
Nouws J,Nijtmans L,Houten SM,van den Brand M,Huynen M,Venselaar H,Hoefs S,Gloerich J,Kronick J,Hutchin T,Willems P,Rodenburg R,Wanders R,van den Heuvel L,Smeitink J,Vogel RO

Carnitine palmitoyltransferase I and Acyl-CoA dehydrogenase 9 in retina: insights of retinopathy in mitochondrial trifunctional protein defects.
Investigative ophthalmology & visual science49(4)1660-4(2008 Apr)
Roomets E,Kivel? T,Tyni T

Human acyl-CoA dehydrogenase-9 plays a novel role in the mitochondrial beta-oxidation of unsaturated fatty acids.
The Journal of biological chemistry280(37)32309-16(2005 Sep)
Ensenauer R,He M,Willard JM,Goetzman ES,Corydon TJ,Vandahl BB,Mohsen AW,Isaya G,Vockley J

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Acad9 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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