Dio3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dio3
Entrez Gene ID	29475
Full Name	iodothyronine deiodinase 3
Synonyms	5DIII,DIOIII
General protein information	Preferred Names iodothyronine deiodinase 3 Names thyroxine 5-deiodinase deiodinase, iodothyronine, type 3 deiodinase, iodothyronine, type III type 3 DI type III iodothyronine deiodinase type-III 5'-deiodinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q32
Summary	This is an intronless, imprinted gene that is preferentially expressed from the paternal allele. The encoded protein belongs to the iodothyronine deiodinase family, and catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5' triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. It is highly expressed in placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It thus plays a critical role in mammalian development by regulating circulating fetal thyroid hormone concentration. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017210.4	NP_058906.3	thyroxine 5-deiodinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO3	NP_001353.4
Rattus norvegicus (Norway rat)	Dio3	NP_058906.3
Gallus gallus (chicken)	DIO3	NP_001116120.1
Xenopus tropicalis (tropical clawed frog)	dio3	NP_001107139.2
	DIO3	NP_001116121.1
Canis lupus familiaris (dog)	DIO3	NP_001157660.1
Danio rerio (zebrafish)	dio3a	NP_001242932.1
Bos taurus (cattle)	DIO3	NP_001010993.2
Mus musculus (house mouse)	Dio3	NP_742117.2
Danio rerio (zebrafish)	dio3b	NP_001171406.2

Gene Ontology
Bibliography

Related articles in PubMed

Crystal structure of mammalian selenocysteine-dependent iodothyronine deiodinase suggests a peroxiredoxin-like catalytic mechanism.
Schweizer U, Schlicker C, Braun D, K?hrle J, Steegborn C
Proceedings of the National Academy of Sciences of the United States of America111(29)10526-31(2014 Jul)

Decreased translation of Dio3 mRNA is associated with drug-induced hepatotoxicity.
Dudek KM, Suter L, Darras VM, Marczylo EL, Gant TW
The Biochemical journal453(1)71-82(2013 Jul)

Neuronal hypoxia induces Hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism.
Jo S, Kall? I, Bard?czi Z, Arrojo e Drigo R, Ze?ld A, Liposits Z, Oliva A, Lemmon VP, Bixby JL, Gereben B, Bianco AC
The Journal of neuroscience : the official journal of the Society for Neuroscience32(25)8491-500(2012 Jun)

Strain-specific vulnerability to alcohol exposure in utero via hippocampal parent-of-origin expression of deiodinase-III.
Sittig LJ, Shukla PK, Herzing LB, Redei EE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology25(7)2313-24(2011 Jul)

Gene expression from the imprinted Dio3 locus is associated with cell proliferation of cultured brown adipocytes.
Hernandez A, Garcia B, Obregon MJ
Endocrinology148(8)3968-76(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings suggest that translational repression of Dio3 is an important determinant of the reduced D3 protein expression following liver damage
Title: Decreased translation of Dio3 mRNA is associated with drug-induced hepatotoxicity.

ischemia/hypoxia induces an Hsp40-mediated translocation of Dio3 to the nucleus, facilitating thyroid hormone inactivation proximal to the thyroid hormone receptors
Title: Neuronal hypoxia induces Hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism.

Data suggest that genetic background and sex modify vulnerability to prenatal alcohol via brain region-specific expression of Dio3.
Title: Strain-specific vulnerability to alcohol exposure in utero via hippocampal parent-of-origin expression of deiodinase-III.

we show marked expression of D3 by granulocytes and macrophages in spinal cord inflammatory lesions of EAE rats. We further confirm induction of D3 expression in vitro in monocytes that were activated toward proinflammatory or immunomodulatory phenotypes
Title: Type 3 deiodinase expression in inflammatory spinal cord lesions in rat experimental autoimmune encephalomyelitis.

Dio3 is important in the modulation of thyroid hormone levels in the regenerating liver, in which decrease in cellular T3 permits an increase in proliferation.
Title: Large induction of type III deiodinase expression after partial hepatectomy in the regenerating mouse and rat liver.

The following Dio3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dio3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa63246	NM_017210.4 Latest version!	Rattus norvegicus iodothyronine deiodinase 3 (Dio3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dio3 ( NM_017210.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058906.3 Rattus norvegicus iodothyronine deiodinase 3 (Dio3), mRNA.

Price & Availability↑

CloneID	ORa63246
Clone ID Related Accession (Same CDS sequence)	NM_017210.4
Accession Version	NM_017210.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Rattus norvegicus(Norway rat)
Product	thyroxine 5-deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07065559.1. On Jun 24, 2016 this sequence version replaced NM_017210.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## imprinted gene :: PMID: 17510246 protein contains selenocysteine :: PMID: 7622463 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTCGCC AGGCCGCCTC GAGGTTGGTG GTCGGAGAAG GTGAAGGGCC CCCGGGGGCT 
TCGGGGCCCG CGGCCACCAT GCTGCGCTCC CTGCTGCTTC ACTCTCTGAG GCTCTGCGCC 
CAGACCGCCT CGTGCCTCGT GCTGTTCCCG CGCTTCCTAG GCACGGCCTT CATGCTCTGG 
CTTTTAGATT TCTTGTGCAT CCGCAAGCAT TTCCTGCGCC GTCGTCATCC TGACCACCCC 
GAGCCCGAAG TAGAGCTCAA CAGTGAAGGT GAGGAGATGC CCCCTGACGA CCCACCCATC 
TGCGTATCCG ACGACAACCG GCTGTGCACC CTGGCCTCTC TGAAGGCCGT GTGGCATGGC 
CAGAAGTTGG ATTTCTTCAA GCAGGCCCAT GAGGGTGGCC CAGCACCTAA CTCGGAGGTC 
GTCCGGCCTG ATGGCTTCCA GAGCCAGCGC ATCCTCGACT ACGCACAAGG GACCCGCCCG 
TTGGTGCTCA ATTTTGGCAG CTGTACCTGA CCACCGTTCA TGGCGCGGAT GAGCGCCTTC 
CAGCGCCTGG TCACCAAGTA CCAGCGCGAC GTTGACTTCC TTATCATCTA CATCGAGGAA 
GCCCACCCAT CCGATGGCTG GGTCACCACA GATTCACCCT ACGTCATCCC ACAGCACCGC 
AGCTTGGAGG ACCGTGTCAG CGCAGCAAGA GTACTTCAGC AAGGCGCACC TGGCTGTGCT 
CTGGTTCTGG ACACGATGGC CAACTCCAGC AGTTCCGCAT ATGGTGCCTA TTTTGAGCGC 
CTCTACGTCA TCCAGAGCGG CACCATCATG TACCAGGGAG GCCGTGGCCC CGACGGTTAC 
CAGGTGTCTG AGTTGCGCAC TTGGTTGGAG CGCTATGACG AGCAGTTGCA TGGTACTAGG 
CCACGTCGAC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058906.3
CDS	3..917
Translation	MPRQAASRLVVGEGEGPPGASGPAATMLRSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLRRRHPDHPEPEVELNSEGEEMPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVRPDGFQSQRILDYAQGTRPLVLNFGSCT*PPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYVIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLHGTRPRRL

Target ORF information:

RefSeq Version	NM_017210.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus iodothyronine deiodinase 3 (Dio3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017210.4

ORF Insert Sequence:

ATGCCTCGCC AGGCCGCCTC GAGGTTGGTG GTCGGAGAAG GTGAAGGGCC CCCGGGGGCT 
TCGGGGCCCG CGGCCACCAT GCTGCGCTCC CTGCTGCTTC ACTCTCTGAG GCTCTGCGCC 
CAGACCGCCT CGTGCCTCGT GCTGTTCCCG CGCTTCCTAG GCACGGCCTT CATGCTCTGG 
CTTTTAGATT TCTTGTGCAT CCGCAAGCAT TTCCTGCGCC GTCGTCATCC TGACCACCCC 
GAGCCCGAAG TAGAGCTCAA CAGTGAAGGT GAGGAGATGC CCCCTGACGA CCCACCCATC 
TGCGTATCCG ACGACAACCG GCTGTGCACC CTGGCCTCTC TGAAGGCCGT GTGGCATGGC 
CAGAAGTTGG ATTTCTTCAA GCAGGCCCAT GAGGGTGGCC CAGCACCTAA CTCGGAGGTC 
GTCCGGCCTG ATGGCTTCCA GAGCCAGCGC ATCCTCGACT ACGCACAAGG GACCCGCCCG 
TTGGTGCTCA ATTTTGGCAG CTGTACCTGA CCACCGTTCA TGGCGCGGAT GAGCGCCTTC 
CAGCGCCTGG TCACCAAGTA CCAGCGCGAC GTTGACTTCC TTATCATCTA CATCGAGGAA 
GCCCACCCAT CCGATGGCTG GGTCACCACA GATTCACCCT ACGTCATCCC ACAGCACCGC 
AGCTTGGAGG ACCGTGTCAG CGCAGCAAGA GTACTTCAGC AAGGCGCACC TGGCTGTGCT 
CTGGTTCTGG ACACGATGGC CAACTCCAGC AGTTCCGCAT ATGGTGCCTA TTTTGAGCGC 
CTCTACGTCA TCCAGAGCGG CACCATCATG TACCAGGGAG GCCGTGGCCC CGACGGTTAC 
CAGGTGTCTG AGTTGCGCAC TTGGTTGGAG CGCTATGACG AGCAGTTGCA TGGTACTAGG 
CCACGTCGAC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal structure of mammalian selenocysteine-dependent iodothyronine deiodinase suggests a peroxiredoxin-like catalytic mechanism.
Proceedings of the National Academy of Sciences of the United States of America111(29)10526-31(2014 Jul)
Schweizer U,Schlicker C,Braun D,K?hrle J,Steegborn C

Decreased translation of Dio3 mRNA is associated with drug-induced hepatotoxicity.
The Biochemical journal453(1)71-82(2013 Jul)
Dudek KM,Suter L,Darras VM,Marczylo EL,Gant TW

Neuronal hypoxia induces Hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism.
The Journal of neuroscience : the official journal of the Society for Neuroscience32(25)8491-500(2012 Jun)
Jo S,Kall? I,Bard?czi Z,Arrojo e Drigo R,Ze?ld A,Liposits Z,Oliva A,Lemmon VP,Bixby JL,Gereben B,Bianco AC

Strain-specific vulnerability to alcohol exposure in utero via hippocampal parent-of-origin expression of deiodinase-III.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology25(7)2313-24(2011 Jul)
Sittig LJ,Shukla PK,Herzing LB,Redei EE

Gene expression from the imprinted Dio3 locus is associated with cell proliferation of cultured brown adipocytes.
Endocrinology148(8)3968-76(2007 Aug)
Hernandez A,Garcia B,Obregon MJ

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Dio3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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