Pkn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pkn1
Entrez Gene ID	29355
Full Name	protein kinase N1
Synonyms	PAK-1,Prkcl1
General protein information	Preferred Names protein kinase N1 Names serine/threonine-protein kinase N1 protease-activated kinase 1 protein kinase C-like 1 protein kinase C-like PKN protein kinase PKN protein-kinase C-related kinase 1 serine-threonine protein kinase N
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q11
Summary	displays phosphorylation dependent protein kinase activity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017601249.1	XP_017456738.1	serine/threonine-protein kinase N1 isoform X1
XM_006255245.2	XP_006255307.1	serine/threonine-protein kinase N1 isoform X2
NM_017175.2	NP_058871.2	serine/threonine-protein kinase N1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PKN1	XP_512443.3
Danio rerio (zebrafish)	LOC560840	XP_005160108.1
Homo sapiens (human)	PKN1	NP_002732.3
Canis lupus familiaris (dog)	PKN1	XP_003639827.1
Bos taurus (cattle)	PKN1	NP_001073715.1
Rattus norvegicus (Norway rat)	Pkn1	NP_058871.2
Danio rerio (zebrafish)	zgc:162290	NP_001082929.1
	LOC718834	XP_002808221.1
Mus musculus (house mouse)	Pkn1	NP_796236.2

Gene Ontology
Bibliography

Related articles in PubMed

Loss of Protein Kinase Novel 1 (PKN1) is associated with mild systolic and diastolic contractile dysfunction, increased phospholamban Thr17 phosphorylation, and exacerbated ischaemia-reperfusion injury.
Francois AA, Obasanjo-Blackshire K, Clark JE, Boguslavskyi A, Holt MR, Parker PJ, Marber MS, Heads RJ
Cardiovascular research114(1)138-157(2018 Jan)

Protein kinase N1, a cell inhibitor of Akt kinase, has a central role in quality control of germinal center formation.
Yasui T, Sakakibara-Yada K, Nishimura T, Morita K, Tada S, Mosialos G, Kieff E, Kikutani H
Proceedings of the National Academy of Sciences of the United States of America109(51)21022-7(2012 Dec)

Protein kinase N1 is a novel substrate of NFATc1-mediated cyclin D1-CDK6 activity and modulates vascular smooth muscle cell division and migration leading to inward blood vessel wall remodeling.
Singh NK, Kundumani-Sridharan V, Kumar S, Verma SK, Kotla S, Mukai H, Heckle MR, Rao GN
The Journal of biological chemistry287(43)36291-304(2012 Oct)

Regulatory domain selectivity in the cell-type specific PKN-dependence of cell migration.
Lachmann S, Jevons A, De Rycker M, Casamassima A, Radtke S, Collazos A, Parker PJ
PloS one6(7)e21732(2011)

Phosphorylation of histone H3T6 by PKCbeta(I) controls demethylation at histone H3K4.
Metzger E, Imhof A, Patel D, Kahl P, Hoffmeyer K, Friedrichs N, M?ller JM, Greschik H, Kirfel J, Ji S, Kunowska N, Beisenherz-Huss C, G?nther T, Buettner R, Sch?le R
Nature464(7289)792-6(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Protein kinase N1 is a novel substrate of NFATc1-mediated cyclin D1-CDK6 activity and modulates vascular smooth muscle cell division and migration leading to inward blood vessel wall remodeling.
Title: Protein kinase N1 is a novel substrate of NFATc1-mediated cyclin D1-CDK6 activity and modulates vascular smooth muscle cell division and migration leading to inward blood vessel wall remodeling.

Pak1 acts on polarity in a kinase-dependent manner, by affecting the F-actin and microtubule cytoskeleton at least in part through Rac1 and cofilin.
Title: Localized activation of p21-activated kinase controls neuronal polarity and morphology.

Data show that the HR1 region is necessary but not sufficient in eliciting a full activation of PRK1 upon binding of RhoA, and that activation is controlled by the very C-terminus of PRK1.
Title: The very C-terminus of PRK1/PKN is essential for its activation by RhoA and downstream signaling.

The following Pkn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pkn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13174	NM_017175.2 Latest version!	Rattus norvegicus protein kinase N1 (Pkn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa38696	XM_006255245.2 Latest version!	Rattus norvegicus protein kinase N1 (Pkn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa59419	XM_017601249.1 Latest version!	Rattus norvegicus protein kinase N1 (Pkn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pkn1 ( NM_017175.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058871.2 Rattus norvegicus protein kinase N1 (Pkn1), mRNA.

Price & Availability↑

CloneID	ORa13174
Clone ID Related Accession (Same CDS sequence)	NM_017175.2
Accession Version	NM_017175.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase N1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB608722.1, D26180.1, BC061836.1 and EV774518.1. On Jan 24, 2008 this sequence version replaced NM_017175.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ222217.1, D26180.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGGCG ACGCCGTGCA GAGTGAACCT CGCAGCTGGT CACTGCTGGA GCAGCTGGGT 
CTGGCTGGGG CAGACCTGGC AGCCCCTGGG GTGCAGCAGC AGCTGGAGTT AGAGCGAGAG 
CGGCTGAAGC GGGAAATCCG AAAAGAGCTG AAGCTGAAGG AGGGCGCTGA GAACCTGAGG 
CGGGCCACCA CTGACCTGGG CCGCAGCTTG GCCCCTGTGG AACTGCTGCT GAGGGGCTCC 
GCTAGACGGC TTGACTTGCT GCACCAGCAG CTGCAGGAGC TGCATGCACA TGTGGTGCTG 
CCCGACCCTA CAGCGGGGAG TGATGCTCCC CAATCCCTTG CAGAGGGCAG CCCTGTCTGC 
TCATCCACCA ACCTGAGCCG AGTGGCTGGC CTGGAGAAGC AGCTGGCCAT TGAGCTCAAG 
GTCAAACAGG GGGCAGAAAA CATGATCCAG ACCTACAGCA ATGGCAGCAC CAAGGACCGG 
AAGCTGCTGT TGACGGCCCA ACAGATGCTG CAGGATAGTA AGACCAAGAT TGACATCATC 
CGCATGCAGC TTCGCCGGGC GCTACAAGCA CTCCAGGCTG GCCAGCTGGA GAGTCAGGCA 
GCTCCTGATG AGGCCCACGG AGATCCAGAC CTGGGAGCCG TAGAGCTACG CATTGAGGAG 
CTACGACACC ATTTTCGAGT AGAGCATGCA GTGGCAGAAG GCGCCAAGAA TGTCCTGCGT 
CTGCTCAGTG CTGCAAAGGC CCCAGACCGC AAAGCAGTCA GCGAGGCTCA GGAGAAATTG 
ACTGAGTCCA ACCAGAAGCT GGGCTTGCTG CGGGAGTCAC TGGAGAGGCG CTTGGGGGAA 
CTGCCTGCTG ATCACCCCAA GGGACGCCTG CTTCGGGAGG AGCTCACTGC GGCCTCATCG 
GCAGCCTTCA GTGCAATACT GCCTGGGCCC TTCCCTGCCA CTCACTACAG CACCTTGAGC 
AAGCCTGCAC CACTCACAGG GACCCTGGAA GTACGAGTGG TGGGCTGCAA AAACCTTCCC 
GAGACCATCC CTTGGAGCCC TCCCCCCTCA GTCGGGGCAT CTGGGACCCC CGACAGCCGC 
ACTCCTTTCC TGAGTCGTCC AGCTCGGGGC CTTTACAACC GAAGTGGAAG CCTTAGTGGA 
CGGAGCAGCC TCAAGGGGGA GGCAGAGAAT TCCACTGAGG TCAGCACCGT GCTCAAGCTG 
GACAACACTG TGGTGGGGCA AACAGCCTGG AAGCCATGCG GCCCCAATGC CTGGGACCAG 
AGCTTCACCC TGGAGCTGGA GAGGGCTCGG GAGCTGGAGT TGGCTGTGTT CTGGCGTGAC 
CAGAGGGGTC TGTGTGCTCT CAAATTTCTG AAGTTGGAAG ACTTCTTGGA CAATGAGAGG 
CATGAGGTGC AGCTGGACAT GGAACCCCAG GGCTGCCTGG TGGCTGAGGT CACCTTCCGT 
AACCCCATCA TCGAGCGGAT CCCTAGGCTC CAAAGGCAGA AAAAAATTTT CTCCAAGCAG 
CAAGGGCAGA CATTTCAGCG TGCCAGACAG ATGAACATCG ATGTGGCCAC CTGGGTGCGG 
CTGCTCCGGA GACTCATCCC GAACGCCGTG GCCACTGGCT CCTTCAGCCC CAATGCATCT 
CCAGGCTCTG AGATCCGGAG CACTGGAGAC ATATCCATGG AGAAATTGAA TCTCGGTGCT 
GACTCAGACA GCTCGTCCCA GAAGAGCCCC GCAGGGCTGC CCTCCACCTC ATGTAGCCTG 
AGTTCTCCAA CCCACGAATC CACCACCTCT CCAGAGCTGC CTTCAGAGAC CCAGGAGACC 
CCAGGCCCTG GCCTGTGCAG TCCCCTGAGG AAGTCGCCCC TGACGCTTGA GGACTTCAAG 
TTCCTGGCAG TGCTTGGTCG GGGTCACTTT GGAAAGGTGC TGCTGTCTGA ATTCCACTCC 
AGTGGGGAGC TCTTTGCCAT TAAAGCCTTG AAGAAAGGTG ACATTGTAGC CCGGGATGAG 
GTTGAGAGCC TGATGTGTGA GAAGCGGATT TTGGCGACCG TGACCAGGGC AGGACATCCC 
TTCCTGGTGA ACCTTTTCGG CTGTTTCCAG ACCCCAGAGC ATGTGTGCTT TGTGATGGAG 
TACTCAGCCG GTGGGGACTT GATGCTGCAT ATCCACAGCG ACGTGTTCTC AGAGCCTCGG 
GCTGTCTTCT ATTCGGCCTG TGTGGTGCTG GGACTGCAGT TCCTCCATGA ACACAAGATT 
GTCTACAGGG ACCTGAAGTT GGACAATTTG CTCCTGGATA CTGAGGGCTA CGTCAAGATC 
GCAGACTTTG GCCTCTGCAA GGAGGGGATG GGCTATGGGG ACCGGACCAG CACATTCTGC 
GGAACTCCGG AGTTCCTGGC GCCGGAAGTG CTCACAGACA CATCCTACAC TCGAGCCGTG 
GACTGGTGGG GACTGGGTGT ATTGCTCTAT GAGATGCTGG TTGGAGAGTC TCCGTTCCCT 
GGGGACGACG AGGAGGAAGT ATTTGACAGC ATCGTCAATG ATGAGGTTCG TTATCCCCGC 
TTCCTGTCTG CGGAGGCCAT CGGCATCATG AGAAGGCTAC TGCGGAGGAA CCCAGAGCGG 
AGGTTGGGAT CCACTGAGCG TGATGCAGAA GATGTGAAAA AACAGCCTTT CTTCAGGACT 
CTGGACTGGG ATGCCCTGCT GGCCCGTCGC CTGCCTCCAC CCTTCGTGCC TACACTTTCG 
GGGCGCACAG ACGTCAGCAA CTTCGATGAG GAGTTCACTG GGGAGGCCCC CACACTGAGC 
CCTCCCCGGG ATGCACGGCC CCTGACAGCT GCGGAGCAGG CGGCCTTCCG GGATTTCGAC 
TTTGTGGCAG GAGGCTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058871.2
CDS	175..3015
Translation	MAGDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLKREIRKELKLKEGAENLRRATTDLGRSLAPVELLLRGSARRLDLLHQQLQELHAHVVLPDPTAGSDAPQSLAEGSPVCSSTNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQALQAGQLESQAAPDEAHGDPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLRESLERRLGELPADHPKGRLLREELTAASSAAFSAILPGPFPATHYSTLSKPAPLTGTLEVRVVGCKNLPETIPWSPPPSVGASGTPDSRTPFLSRPARGLYNRSGSLSGRSSLKGEAENSTEVSTVLKLDNTVVGQTAWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPIIERIPRLQRQKKIFSKQQGQTFQRARQMNIDVATWVRLLRRLIPNAVATGSFSPNASPGSEIRSTGDISMEKLNLGADSDSSSQKSPAGLPSTSCSLSSPTHESTTSPELPSETQETPGPGLCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFHSSGELFAIKALKKGDIVARDEVESLMCEKRILATVTRAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAVFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSTERDAEDVKKQPFFRTLDWDALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFRDFDFVAGGY

Target ORF information:

RefSeq Version	NM_017175.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase N1 (Pkn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017175.2

ORF Insert Sequence:

ATGGCCGGCG ACGCCGTGCA GAGTGAACCT CGCAGCTGGT CACTGCTGGA GCAGCTGGGT 
CTGGCTGGGG CAGACCTGGC AGCCCCTGGG GTGCAGCAGC AGCTGGAGTT AGAGCGAGAG 
CGGCTGAAGC GGGAAATCCG AAAAGAGCTG AAGCTGAAGG AGGGCGCTGA GAACCTGAGG 
CGGGCCACCA CTGACCTGGG CCGCAGCTTG GCCCCTGTGG AACTGCTGCT GAGGGGCTCC 
GCTAGACGGC TTGACTTGCT GCACCAGCAG CTGCAGGAGC TGCATGCACA TGTGGTGCTG 
CCCGACCCTA CAGCGGGGAG TGATGCTCCC CAATCCCTTG CAGAGGGCAG CCCTGTCTGC 
TCATCCACCA ACCTGAGCCG AGTGGCTGGC CTGGAGAAGC AGCTGGCCAT TGAGCTCAAG 
GTCAAACAGG GGGCAGAAAA CATGATCCAG ACCTACAGCA ATGGCAGCAC CAAGGACCGG 
AAGCTGCTGT TGACGGCCCA ACAGATGCTG CAGGATAGTA AGACCAAGAT TGACATCATC 
CGCATGCAGC TTCGCCGGGC GCTACAAGCA CTCCAGGCTG GCCAGCTGGA GAGTCAGGCA 
GCTCCTGATG AGGCCCACGG AGATCCAGAC CTGGGAGCCG TAGAGCTACG CATTGAGGAG 
CTACGACACC ATTTTCGAGT AGAGCATGCA GTGGCAGAAG GCGCCAAGAA TGTCCTGCGT 
CTGCTCAGTG CTGCAAAGGC CCCAGACCGC AAAGCAGTCA GCGAGGCTCA GGAGAAATTG 
ACTGAGTCCA ACCAGAAGCT GGGCTTGCTG CGGGAGTCAC TGGAGAGGCG CTTGGGGGAA 
CTGCCTGCTG ATCACCCCAA GGGACGCCTG CTTCGGGAGG AGCTCACTGC GGCCTCATCG 
GCAGCCTTCA GTGCAATACT GCCTGGGCCC TTCCCTGCCA CTCACTACAG CACCTTGAGC 
AAGCCTGCAC CACTCACAGG GACCCTGGAA GTACGAGTGG TGGGCTGCAA AAACCTTCCC 
GAGACCATCC CTTGGAGCCC TCCCCCCTCA GTCGGGGCAT CTGGGACCCC CGACAGCCGC 
ACTCCTTTCC TGAGTCGTCC AGCTCGGGGC CTTTACAACC GAAGTGGAAG CCTTAGTGGA 
CGGAGCAGCC TCAAGGGGGA GGCAGAGAAT TCCACTGAGG TCAGCACCGT GCTCAAGCTG 
GACAACACTG TGGTGGGGCA AACAGCCTGG AAGCCATGCG GCCCCAATGC CTGGGACCAG 
AGCTTCACCC TGGAGCTGGA GAGGGCTCGG GAGCTGGAGT TGGCTGTGTT CTGGCGTGAC 
CAGAGGGGTC TGTGTGCTCT CAAATTTCTG AAGTTGGAAG ACTTCTTGGA CAATGAGAGG 
CATGAGGTGC AGCTGGACAT GGAACCCCAG GGCTGCCTGG TGGCTGAGGT CACCTTCCGT 
AACCCCATCA TCGAGCGGAT CCCTAGGCTC CAAAGGCAGA AAAAAATTTT CTCCAAGCAG 
CAAGGGCAGA CATTTCAGCG TGCCAGACAG ATGAACATCG ATGTGGCCAC CTGGGTGCGG 
CTGCTCCGGA GACTCATCCC GAACGCCGTG GCCACTGGCT CCTTCAGCCC CAATGCATCT 
CCAGGCTCTG AGATCCGGAG CACTGGAGAC ATATCCATGG AGAAATTGAA TCTCGGTGCT 
GACTCAGACA GCTCGTCCCA GAAGAGCCCC GCAGGGCTGC CCTCCACCTC ATGTAGCCTG 
AGTTCTCCAA CCCACGAATC CACCACCTCT CCAGAGCTGC CTTCAGAGAC CCAGGAGACC 
CCAGGCCCTG GCCTGTGCAG TCCCCTGAGG AAGTCGCCCC TGACGCTTGA GGACTTCAAG 
TTCCTGGCAG TGCTTGGTCG GGGTCACTTT GGAAAGGTGC TGCTGTCTGA ATTCCACTCC 
AGTGGGGAGC TCTTTGCCAT TAAAGCCTTG AAGAAAGGTG ACATTGTAGC CCGGGATGAG 
GTTGAGAGCC TGATGTGTGA GAAGCGGATT TTGGCGACCG TGACCAGGGC AGGACATCCC 
TTCCTGGTGA ACCTTTTCGG CTGTTTCCAG ACCCCAGAGC ATGTGTGCTT TGTGATGGAG 
TACTCAGCCG GTGGGGACTT GATGCTGCAT ATCCACAGCG ACGTGTTCTC AGAGCCTCGG 
GCTGTCTTCT ATTCGGCCTG TGTGGTGCTG GGACTGCAGT TCCTCCATGA ACACAAGATT 
GTCTACAGGG ACCTGAAGTT GGACAATTTG CTCCTGGATA CTGAGGGCTA CGTCAAGATC 
GCAGACTTTG GCCTCTGCAA GGAGGGGATG GGCTATGGGG ACCGGACCAG CACATTCTGC 
GGAACTCCGG AGTTCCTGGC GCCGGAAGTG CTCACAGACA CATCCTACAC TCGAGCCGTG 
GACTGGTGGG GACTGGGTGT ATTGCTCTAT GAGATGCTGG TTGGAGAGTC TCCGTTCCCT 
GGGGACGACG AGGAGGAAGT ATTTGACAGC ATCGTCAATG ATGAGGTTCG TTATCCCCGC 
TTCCTGTCTG CGGAGGCCAT CGGCATCATG AGAAGGCTAC TGCGGAGGAA CCCAGAGCGG 
AGGTTGGGAT CCACTGAGCG TGATGCAGAA GATGTGAAAA AACAGCCTTT CTTCAGGACT 
CTGGACTGGG ATGCCCTGCT GGCCCGTCGC CTGCCTCCAC CCTTCGTGCC TACACTTTCG 
GGGCGCACAG ACGTCAGCAA CTTCGATGAG GAGTTCACTG GGGAGGCCCC CACACTGAGC 
CCTCCCCGGG ATGCACGGCC CCTGACAGCT GCGGAGCAGG CGGCCTTCCG GGATTTCGAC 
TTTGTGGCAG GAGGCTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkn1 ( XM_006255245.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255307.1 Rattus norvegicus protein kinase N1 (Pkn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa38696
Clone ID Related Accession (Same CDS sequence)	XM_006255245.2
Accession Version	XM_006255245.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase N1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006255245.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG ATCCCCCTTT GGATTTGGAG CTGGAGAGTG AACCTCGCAG CTGGTCACTG 
CTGGAGCAGC TGGGTCTGGC TGGGGCAGAC CTGGCAGCCC CTGGGGTGCA GCAGCAGCTG 
GAGTTAGAGC GAGAGCGGCT GAAGCGGGAA ATCCGAAAAG AGCTGAAGCT GAAGGAGGGC 
GCTGAGAACC TGAGGCGGGC CACCACTGAC CTGGGCCGCA GCTTGGCCCC TGTGGAACTG 
CTGCTGAGGG GCTCCGCTAG ACGGCTTGAC TTGCTGCACC AGCAGCTGCA GGAGCTGCAT 
GCACATGTGG TGCTGCCCGA CCCTACAGCG GGGAGTGATG CTCCCCAATC CCTTGCAGAG 
GGCAGCCCTG TCTGCTCATC CACCAACCTG AGCCGAGTGG CTGGCCTGGA GAAGCAGCTG 
GCCATTGAGC TCAAGGTCAA ACAGGGGGCA GAAAACATGA TCCAGACCTA CAGCAATGGC 
AGCACCAAGG ACCGGAAGCT GCTGTTGACG GCCCAACAGA TGCTGCAGGA TAGTAAGACC 
AAGATTGACA TCATCCGCAT GCAGCTTCGC CGGGCGCTAC AAGCACTCCA GGCTGGCCAG 
CTGGAGAGTC AGGCAGCTCC TGATGAGGCC CACGGAGATC CAGACCTGGG AGCCGTAGAG 
CTACGCATTG AGGAGCTACG ACACCATTTT CGAGTAGAGC ATGCAGTGGC AGAAGGCGCC 
AAGAATGTCC TGCGTCTGCT CAGTGCTGCA AAGGCCCCAG ACCGCAAAGC AGTCAGCGAG 
GCTCAGGAGA AATTGACTGA GTCCAACCAG AAGCTGGGCT TGCTGCGGGA GTCACTGGAG 
AGGCGCTTGG GGGAACTGCC TGCTGATCAC CCCAAGGGAC GCCTGCTTCG GGAGGAGCTC 
ACTGCGGCCT CATCGGCAGC CTTCAGTGCA ATACTGCCTG GGCCCTTCCC TGCCACTCAC 
TACAGCACCT TGAGCAAGCC TGCACCACTC ACAGGGACCC TGGAAGTACG AGTGGTGGGC 
TGCAAAAACC TTCCCGAGAC CATCCCTTGG AGCCCTCCCC CCTCAGTCGG GGCATCTGGG 
ACCCCCGACA GCCGCACTCC TTTCCTGAGT CGTCCAGCTC GGGGCCTTTA CAACCGAAGT 
GGAAGCCTTA GTGGACGGAG CAGCCTCAAG GGGGAGGCAG AGAATTCCAC TGAGGTCAGC 
ACCGTGCTCA AGCTGGACAA CACTGTGGTG GGGCAAACAG CCTGGAAGCC ATGCGGCCCC 
AATGCCTGGG ACCAGAGCTT CACCCTGGAG CTGGAGAGGG CTCGGGAGCT GGAGTTGGCT 
GTGTTCTGGC GTGACCAGAG GGGTCTGTGT GCTCTCAAAT TTCTGAAGTT GGAAGACTTC 
TTGGACAATG AGAGGCATGA GGTGCAGCTG GACATGGAAC CCCAGGGCTG CCTGGTGGCT 
GAGGTCACCT TCCGTAACCC CATCATCGAG CGGATCCCTA GGCTCCAAAG GCAGAAAAAA 
ATTTTCTCCA AGCAGCAAGG GCAGACATTT CAGCGTGCCA GACAGATGAA CATCGATGTG 
GCCACCTGGG TGCGGCTGCT CCGGAGACTC ATCCCGAACG CCGTGGCCAC TGGCTCCTTC 
AGCCCCAATG CATCTCCAGG CTCTGAGATC CGGAGCACTG GAGACATATC CATGGAGAAA 
TTGAATCTCG GTGCTGACTC AGACAGCTCG TCCCAGAAGA GCCCCGCAGG GCTGCCCTCC 
ACCTCATGTA GCCTGAGTTC TCCAACCCAC GAATCCACCA CCTCTCCAGA GCTGCCTTCA 
GAGACCCAGG AGACCCCAGG CCCTGGCCTG TGCAGTCCCC TGAGGAAGTC GCCCCTGACG 
CTTGAGGACT TCAAGTTCCT GGCAGTGCTT GGTCGGGGTC ACTTTGGAAA GGTGCTGCTG 
TCTGAATTCC ACTCCAGTGG GGAGCTCTTT GCCATTAAAG CCTTGAAGAA AGGTGACATT 
GTAGCCCGGG ATGAGGTTGA GAGCCTGATG TGTGAGAAGC GGATTTTGGC GACCGTGACC 
AGGGCAGGAC ATCCCTTCCT GGTGAACCTT TTCGGCTGTT TCCAGACCCC AGAGCATGTG 
TGCTTTGTGA TGGAGTACTC AGCCGGTGGG GACTTGATGC TGCATATCCA CAGCGACGTG 
TTCTCAGAGC CTCGGGCTGT CTTCTATTCG GCCTGTGTGG TGCTGGGACT GCAGTTCCTC 
CATGAACACA AGATTGTCTA CAGGGACCTG AAGTTGGACA ATTTGCTCCT GGATACTGAG 
GGCTACGTCA AGATCGCAGA CTTTGGCCTC TGCAAGGAGG GGATGGGCTA TGGGGACCGG 
ACCAGCACAT TCTGCGGAAC TCCGGAGTTC CTGGCGCCGG AAGTGCTCAC AGACACATCC 
TACACTCGAG CCGTGGACTG GTGGGGACTG GGTGTATTGC TCTATGAGAT GCTGGTTGGA 
GAGTCTCCGT TCCCTGGGGA CGACGAGGAG GAAGTATTTG ACAGCATCGT CAATGATGAG 
GTTCGTTATC CCCGCTTCCT GTCTGCGGAG GCCATCGGCA TCATGAGAAG GCTACTGCGG 
AGGAACCCAG AGCGGAGGTT GGGATCCACT GAGCGTGATG CAGAAGATGT GAAAAAACAG 
CCTTTCTTCA GGACTCTGGA CTGGGATGCC CTGCTGGCCC GTCGCCTGCC TCCACCCTTC 
GTGCCTACAC TTTCGGGGCG CACAGACGTC AGCAACTTCG ATGAGGAGTT CACTGGGGAG 
GCCCCCACAC TGAGCCCTCC CCGGGATGCA CGGCCCCTGA CAGCTGCGGA GCAGGCGGCC 
TTCCGGGATT TCGACTTTGT GGCAGGAGGC TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255307.1
CDS	1200..4055
Translation	MAADPPLDLELESEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLKREIRKELKLKEGAENLRRATTDLGRSLAPVELLLRGSARRLDLLHQQLQELHAHVVLPDPTAGSDAPQSLAEGSPVCSSTNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQALQAGQLESQAAPDEAHGDPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLRESLERRLGELPADHPKGRLLREELTAASSAAFSAILPGPFPATHYSTLSKPAPLTGTLEVRVVGCKNLPETIPWSPPPSVGASGTPDSRTPFLSRPARGLYNRSGSLSGRSSLKGEAENSTEVSTVLKLDNTVVGQTAWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPIIERIPRLQRQKKIFSKQQGQTFQRARQMNIDVATWVRLLRRLIPNAVATGSFSPNASPGSEIRSTGDISMEKLNLGADSDSSSQKSPAGLPSTSCSLSSPTHESTTSPELPSETQETPGPGLCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFHSSGELFAIKALKKGDIVARDEVESLMCEKRILATVTRAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAVFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSTERDAEDVKKQPFFRTLDWDALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFRDFDFVAGGY

Target ORF information:

RefSeq Version	XM_006255245.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase N1 (Pkn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255245.2

ORF Insert Sequence:

ATGGCAGCTG ATCCCCCTTT GGATTTGGAG CTGGAGAGTG AACCTCGCAG CTGGTCACTG 
CTGGAGCAGC TGGGTCTGGC TGGGGCAGAC CTGGCAGCCC CTGGGGTGCA GCAGCAGCTG 
GAGTTAGAGC GAGAGCGGCT GAAGCGGGAA ATCCGAAAAG AGCTGAAGCT GAAGGAGGGC 
GCTGAGAACC TGAGGCGGGC CACCACTGAC CTGGGCCGCA GCTTGGCCCC TGTGGAACTG 
CTGCTGAGGG GCTCCGCTAG ACGGCTTGAC TTGCTGCACC AGCAGCTGCA GGAGCTGCAT 
GCACATGTGG TGCTGCCCGA CCCTACAGCG GGGAGTGATG CTCCCCAATC CCTTGCAGAG 
GGCAGCCCTG TCTGCTCATC CACCAACCTG AGCCGAGTGG CTGGCCTGGA GAAGCAGCTG 
GCCATTGAGC TCAAGGTCAA ACAGGGGGCA GAAAACATGA TCCAGACCTA CAGCAATGGC 
AGCACCAAGG ACCGGAAGCT GCTGTTGACG GCCCAACAGA TGCTGCAGGA TAGTAAGACC 
AAGATTGACA TCATCCGCAT GCAGCTTCGC CGGGCGCTAC AAGCACTCCA GGCTGGCCAG 
CTGGAGAGTC AGGCAGCTCC TGATGAGGCC CACGGAGATC CAGACCTGGG AGCCGTAGAG 
CTACGCATTG AGGAGCTACG ACACCATTTT CGAGTAGAGC ATGCAGTGGC AGAAGGCGCC 
AAGAATGTCC TGCGTCTGCT CAGTGCTGCA AAGGCCCCAG ACCGCAAAGC AGTCAGCGAG 
GCTCAGGAGA AATTGACTGA GTCCAACCAG AAGCTGGGCT TGCTGCGGGA GTCACTGGAG 
AGGCGCTTGG GGGAACTGCC TGCTGATCAC CCCAAGGGAC GCCTGCTTCG GGAGGAGCTC 
ACTGCGGCCT CATCGGCAGC CTTCAGTGCA ATACTGCCTG GGCCCTTCCC TGCCACTCAC 
TACAGCACCT TGAGCAAGCC TGCACCACTC ACAGGGACCC TGGAAGTACG AGTGGTGGGC 
TGCAAAAACC TTCCCGAGAC CATCCCTTGG AGCCCTCCCC CCTCAGTCGG GGCATCTGGG 
ACCCCCGACA GCCGCACTCC TTTCCTGAGT CGTCCAGCTC GGGGCCTTTA CAACCGAAGT 
GGAAGCCTTA GTGGACGGAG CAGCCTCAAG GGGGAGGCAG AGAATTCCAC TGAGGTCAGC 
ACCGTGCTCA AGCTGGACAA CACTGTGGTG GGGCAAACAG CCTGGAAGCC ATGCGGCCCC 
AATGCCTGGG ACCAGAGCTT CACCCTGGAG CTGGAGAGGG CTCGGGAGCT GGAGTTGGCT 
GTGTTCTGGC GTGACCAGAG GGGTCTGTGT GCTCTCAAAT TTCTGAAGTT GGAAGACTTC 
TTGGACAATG AGAGGCATGA GGTGCAGCTG GACATGGAAC CCCAGGGCTG CCTGGTGGCT 
GAGGTCACCT TCCGTAACCC CATCATCGAG CGGATCCCTA GGCTCCAAAG GCAGAAAAAA 
ATTTTCTCCA AGCAGCAAGG GCAGACATTT CAGCGTGCCA GACAGATGAA CATCGATGTG 
GCCACCTGGG TGCGGCTGCT CCGGAGACTC ATCCCGAACG CCGTGGCCAC TGGCTCCTTC 
AGCCCCAATG CATCTCCAGG CTCTGAGATC CGGAGCACTG GAGACATATC CATGGAGAAA 
TTGAATCTCG GTGCTGACTC AGACAGCTCG TCCCAGAAGA GCCCCGCAGG GCTGCCCTCC 
ACCTCATGTA GCCTGAGTTC TCCAACCCAC GAATCCACCA CCTCTCCAGA GCTGCCTTCA 
GAGACCCAGG AGACCCCAGG CCCTGGCCTG TGCAGTCCCC TGAGGAAGTC GCCCCTGACG 
CTTGAGGACT TCAAGTTCCT GGCAGTGCTT GGTCGGGGTC ACTTTGGAAA GGTGCTGCTG 
TCTGAATTCC ACTCCAGTGG GGAGCTCTTT GCCATTAAAG CCTTGAAGAA AGGTGACATT 
GTAGCCCGGG ATGAGGTTGA GAGCCTGATG TGTGAGAAGC GGATTTTGGC GACCGTGACC 
AGGGCAGGAC ATCCCTTCCT GGTGAACCTT TTCGGCTGTT TCCAGACCCC AGAGCATGTG 
TGCTTTGTGA TGGAGTACTC AGCCGGTGGG GACTTGATGC TGCATATCCA CAGCGACGTG 
TTCTCAGAGC CTCGGGCTGT CTTCTATTCG GCCTGTGTGG TGCTGGGACT GCAGTTCCTC 
CATGAACACA AGATTGTCTA CAGGGACCTG AAGTTGGACA ATTTGCTCCT GGATACTGAG 
GGCTACGTCA AGATCGCAGA CTTTGGCCTC TGCAAGGAGG GGATGGGCTA TGGGGACCGG 
ACCAGCACAT TCTGCGGAAC TCCGGAGTTC CTGGCGCCGG AAGTGCTCAC AGACACATCC 
TACACTCGAG CCGTGGACTG GTGGGGACTG GGTGTATTGC TCTATGAGAT GCTGGTTGGA 
GAGTCTCCGT TCCCTGGGGA CGACGAGGAG GAAGTATTTG ACAGCATCGT CAATGATGAG 
GTTCGTTATC CCCGCTTCCT GTCTGCGGAG GCCATCGGCA TCATGAGAAG GCTACTGCGG 
AGGAACCCAG AGCGGAGGTT GGGATCCACT GAGCGTGATG CAGAAGATGT GAAAAAACAG 
CCTTTCTTCA GGACTCTGGA CTGGGATGCC CTGCTGGCCC GTCGCCTGCC TCCACCCTTC 
GTGCCTACAC TTTCGGGGCG CACAGACGTC AGCAACTTCG ATGAGGAGTT CACTGGGGAG 
GCCCCCACAC TGAGCCCTCC CCGGGATGCA CGGCCCCTGA CAGCTGCGGA GCAGGCGGCC 
TTCCGGGATT TCGACTTTGT GGCAGGAGGC TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkn1 ( XM_017601249.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456738.1 Rattus norvegicus protein kinase N1 (Pkn1), transcript variant X1, mRNA.

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CloneID	ORa59419
Clone ID Related Accession (Same CDS sequence)	XM_017601249.1
Accession Version	XM_017601249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase N1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCT CTATGCAGCT AGGCGGTGTG TGTAGGGAGG TGGATCGTGA CTTTTGCTTT 
CAGAGGGGTC CGAGGTGGGG GACAAGGTTC GTGAGACTCG CGGCCGCTCA GGCCTGTTCA 
GAGTCCCCCT ACGCTATGAG GAGCGCTTGC CACGCAGCAC AGCGAACAGA GATCCCACTC 
CCCGCTGCAA GCCCCGGGGG CCGCCGGGAG CCCCGCCCCC GCCCCCGCCC CGCCTCCTCC 
CCAGGCGCGG ACCTCGGCGC TCACGTGACC CATCCTGCAG CCGCTGGGGC CCGAGGGCGC 
AGCTCGGCGG AGGCCTGGCC AGGCCGGGCC GAGCGCAGCA GGCCGGATCG CGCAGCGCGC 
CAGGGAAGGT CTGGGCTCCG CGCTTCCCGC CCCACACGCC GACGCGCTCG CAAGCCCTCC 
CTCGGCGCTG GGACCCCTGG CGGACATGGC CGGCGACGCC GTGCAGTCCT GCTGATGGCC 
TGCCGGCCTT GCCGAGGAGC TGGGCGTATC TGGTGCTGCC GATCTCACAA GAGTGAACCT 
CGCAGCTGGT CACTGCTGGA GCAGCTGGGT CTGGCTGGGG CAGACCTGGC AGCCCCTGGG 
GTGCAGCAGC AGCTGGAGTT AGAGCGAGAG CGGCTGAAGC GGGAAATCCG AAAAGAGCTG 
AAGCTGAAGG AGGGCGCTGA GAACCTGAGG CGGGCCACCA CTGACCTGGG CCGCAGCTTG 
GCCCCTGTGG AACTGCTGCT GAGGGGCTCC GCTAGACGGC TTGACTTGCT GCACCAGCAG 
CTGCAGGAGC TGCATGCACA TGTGGTGCTG CCCGACCCTA CAGCGGGGAG TGATGCTCCC 
CAATCCCTTG CAGAGGGCAG CCCTGTCTGC TCATCCACCA ACCTGAGCCG AGTGGCTGGC 
CTGGAGAAGC AGCTGGCCAT TGAGCTCAAG GTCAAACAGG GGGCAGAAAA CATGATCCAG 
ACCTACAGCA ATGGCAGCAC CAAGGACCGG AAGCTGCTGT TGACGGCCCA ACAGATGCTG 
CAGGATAGTA AGACCAAGAT TGACATCATC CGCATGCAGC TTCGCCGGGC GCTACAAGCA 
CTCCAGGCTG GCCAGCTGGA GAGTCAGGCA GCTCCTGATG AGGCCCACGG AGATCCAGAC 
CTGGGAGCCG TAGAGCTACG CATTGAGGAG CTACGACACC ATTTTCGAGT AGAGCATGCA 
GTGGCAGAAG GCGCCAAGAA TGTCCTGCGT CTGCTCAGTG CTGCAAAGGC CCCAGACCGC 
AAAGCAGTCA GCGAGGCTCA GGAGAAATTG ACTGAGTCCA ACCAGAAGCT GGGCTTGCTG 
CGGGAGTCAC TGGAGAGGCG CTTGGGGGAA CTGCCTGCTG ATCACCCCAA GGGACGCCTG 
CTTCGGGAGG AGCTCACTGC GGCCTCATCG GCAGCCTTCA GTGCAATACT GCCTGGGCCC 
TTCCCTGCCA CTCACTACAG CACCTTGAGC AAGCCTGCAC CACTCACAGG GACCCTGGAA 
GTACGAGTGG TGGGCTGCAA AAACCTTCCC GAGACCATCC CTTGGAGCCC TCCCCCCTCA 
GTCGGGGCAT CTGGGACCCC CGACAGCCGC ACTCCTTTCC TGAGTCGTCC AGCTCGGGGC 
CTTTACAACC GAAGTGGAAG CCTTAGTGGA CGGAGCAGCC TCAAGGGGGA GGCAGAGAAT 
TCCACTGAGG TCAGCACCGT GCTCAAGCTG GACAACACTG TGGTGGGGCA AACAGCCTGG 
AAGCCATGCG GCCCCAATGC CTGGGACCAG AGCTTCACCC TGGAGCTGGA GAGGGCTCGG 
GAGCTGGAGT TGGCTGTGTT CTGGCGTGAC CAGAGGGGTC TGTGTGCTCT CAAATTTCTG 
AAGTTGGAAG ACTTCTTGGA CAATGAGAGG CATGAGGTGC AGCTGGACAT GGAACCCCAG 
GGCTGCCTGG TGGCTGAGGT CACCTTCCGT AACCCCATCA TCGAGCGGAT CCCTAGGCTC 
CAAAGGCAGA AAAAAATTTT CTCCAAGCAG CAAGGGCAGA CATTTCAGCG TGCCAGACAG 
ATGAACATCG ATGTGGCCAC CTGGGTGCGG CTGCTCCGGA GACTCATCCC GAACGCCGTG 
GCCACTGGCT CCTTCAGCCC CAATGCATCT CCAGGCTCTG AGATCCGGAG CACTGGAGAC 
ATATCCATGG AGAAATTGAA TCTCGGTGCT GACTCAGACA GCTCGTCCCA GAAGAGCCCC 
GCAGGGCTGC CCTCCACCTC ATGTAGCCTG AGTTCTCCAA CCCACGAATC CACCACCTCT 
CCAGAGCTGC CTTCAGAGAC CCAGGAGACC CCAGGCCCTG GCCTGTGCAG TCCCCTGAGG 
AAGTCGCCCC TGACGCTTGA GGACTTCAAG TTCCTGGCAG TGCTTGGTCG GGGTCACTTT 
GGAAAGGTGC TGCTGTCTGA ATTCCACTCC AGTGGGGAGC TCTTTGCCAT TAAAGCCTTG 
AAGAAAGGTG ACATTGTAGC CCGGGATGAG GTTGAGAGCC TGATGTGTGA GAAGCGGATT 
TTGGCGACCG TGACCAGGGC AGGACATCCC TTCCTGGTGA ACCTTTTCGG CTGTTTCCAG 
ACCCCAGAGC ATGTGTGCTT TGTGATGGAG TACTCAGCCG GTGGGGACTT GATGCTGCAT 
ATCCACAGCG ACGTGTTCTC AGAGCCTCGG GCTGTCTTCT ATTCGGCCTG TGTGGTGCTG 
GGACTGCAGT TCCTCCATGA ACACAAGATT GTCTACAGGG ACCTGAAGTT GGACAATTTG 
CTCCTGGATA CTGAGGGCTA CGTCAAGATC GCAGACTTTG GCCTCTGCAA GGAGGGGATG 
GGCTATGGGG ACCGGACCAG CACATTCTGC GGAACTCCGG AGTTCCTGGC GCCGGAAGTG 
CTCACAGACA CATCCTACAC TCGAGCCGTG GACTGGTGGG GACTGGGTGT ATTGCTCTAT 
GAGATGCTGG TTGGAGAGTC TCCGTTCCCT GGGGACGACG AGGAGGAAGT ATTTGACAGC 
ATCGTCAATG ATGAGGTTCG TTATCCCCGC TTCCTGTCTG CGGAGGCCAT CGGCATCATG 
AGAAGGCTAC TGCGGAGGAA CCCAGAGCGG AGGTTGGGAT CCACTGAGCG TGATGCAGAA 
GATGTGAAAA AACAGCCTTT CTTCAGGACT CTGGACTGGG ATGCCCTGCT GGCCCGTCGC 
CTGCCTCCAC CCTTCGTGCC TACACTTTCG GGGCGCACAG ACGTCAGCAA CTTCGATGAG 
GAGTTCACTG GGGAGGCCCC CACACTGAGC CCTCCCCGGG ATGCACGGCC CCTGACAGCT 
GCGGAGCAGG CGGCCTTCCG GGATTTCGAC TTTGTGGCAG GAGGCTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456738.1
CDS	535..3885
Translation	MDASMQLGGVCREVDRDFCFQRGPRWGTRFVRLAAAQACSESPYAMRSACHAAQRTEIPLPAASPGGRREPRPRPRPASSPGADLGAHVTHPAAAGARGRSSAEAWPGRAERSRPDRAARQGRSGLRASRPTRRRARKPSLGAGTPGGHGRRRRAVLLMACRPCRGAGRIWCCRSHKSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLKREIRKELKLKEGAENLRRATTDLGRSLAPVELLLRGSARRLDLLHQQLQELHAHVVLPDPTAGSDAPQSLAEGSPVCSSTNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQALQAGQLESQAAPDEAHGDPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLRESLERRLGELPADHPKGRLLREELTAASSAAFSAILPGPFPATHYSTLSKPAPLTGTLEVRVVGCKNLPETIPWSPPPSVGASGTPDSRTPFLSRPARGLYNRSGSLSGRSSLKGEAENSTEVSTVLKLDNTVVGQTAWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPIIERIPRLQRQKKIFSKQQGQTFQRARQMNIDVATWVRLLRRLIPNAVATGSFSPNASPGSEIRSTGDISMEKLNLGADSDSSSQKSPAGLPSTSCSLSSPTHESTTSPELPSETQETPGPGLCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFHSSGELFAIKALKKGDIVARDEVESLMCEKRILATVTRAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAVFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSTERDAEDVKKQPFFRTLDWDALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFRDFDFVAGGY

Target ORF information:

RefSeq Version	XM_017601249.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase N1 (Pkn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601249.1

ORF Insert Sequence:

ATGGATGCCT CTATGCAGCT AGGCGGTGTG TGTAGGGAGG TGGATCGTGA CTTTTGCTTT 
CAGAGGGGTC CGAGGTGGGG GACAAGGTTC GTGAGACTCG CGGCCGCTCA GGCCTGTTCA 
GAGTCCCCCT ACGCTATGAG GAGCGCTTGC CACGCAGCAC AGCGAACAGA GATCCCACTC 
CCCGCTGCAA GCCCCGGGGG CCGCCGGGAG CCCCGCCCCC GCCCCCGCCC CGCCTCCTCC 
CCAGGCGCGG ACCTCGGCGC TCACGTGACC CATCCTGCAG CCGCTGGGGC CCGAGGGCGC 
AGCTCGGCGG AGGCCTGGCC AGGCCGGGCC GAGCGCAGCA GGCCGGATCG CGCAGCGCGC 
CAGGGAAGGT CTGGGCTCCG CGCTTCCCGC CCCACACGCC GACGCGCTCG CAAGCCCTCC 
CTCGGCGCTG GGACCCCTGG CGGACATGGC CGGCGACGCC GTGCAGTCCT GCTGATGGCC 
TGCCGGCCTT GCCGAGGAGC TGGGCGTATC TGGTGCTGCC GATCTCACAA GAGTGAACCT 
CGCAGCTGGT CACTGCTGGA GCAGCTGGGT CTGGCTGGGG CAGACCTGGC AGCCCCTGGG 
GTGCAGCAGC AGCTGGAGTT AGAGCGAGAG CGGCTGAAGC GGGAAATCCG AAAAGAGCTG 
AAGCTGAAGG AGGGCGCTGA GAACCTGAGG CGGGCCACCA CTGACCTGGG CCGCAGCTTG 
GCCCCTGTGG AACTGCTGCT GAGGGGCTCC GCTAGACGGC TTGACTTGCT GCACCAGCAG 
CTGCAGGAGC TGCATGCACA TGTGGTGCTG CCCGACCCTA CAGCGGGGAG TGATGCTCCC 
CAATCCCTTG CAGAGGGCAG CCCTGTCTGC TCATCCACCA ACCTGAGCCG AGTGGCTGGC 
CTGGAGAAGC AGCTGGCCAT TGAGCTCAAG GTCAAACAGG GGGCAGAAAA CATGATCCAG 
ACCTACAGCA ATGGCAGCAC CAAGGACCGG AAGCTGCTGT TGACGGCCCA ACAGATGCTG 
CAGGATAGTA AGACCAAGAT TGACATCATC CGCATGCAGC TTCGCCGGGC GCTACAAGCA 
CTCCAGGCTG GCCAGCTGGA GAGTCAGGCA GCTCCTGATG AGGCCCACGG AGATCCAGAC 
CTGGGAGCCG TAGAGCTACG CATTGAGGAG CTACGACACC ATTTTCGAGT AGAGCATGCA 
GTGGCAGAAG GCGCCAAGAA TGTCCTGCGT CTGCTCAGTG CTGCAAAGGC CCCAGACCGC 
AAAGCAGTCA GCGAGGCTCA GGAGAAATTG ACTGAGTCCA ACCAGAAGCT GGGCTTGCTG 
CGGGAGTCAC TGGAGAGGCG CTTGGGGGAA CTGCCTGCTG ATCACCCCAA GGGACGCCTG 
CTTCGGGAGG AGCTCACTGC GGCCTCATCG GCAGCCTTCA GTGCAATACT GCCTGGGCCC 
TTCCCTGCCA CTCACTACAG CACCTTGAGC AAGCCTGCAC CACTCACAGG GACCCTGGAA 
GTACGAGTGG TGGGCTGCAA AAACCTTCCC GAGACCATCC CTTGGAGCCC TCCCCCCTCA 
GTCGGGGCAT CTGGGACCCC CGACAGCCGC ACTCCTTTCC TGAGTCGTCC AGCTCGGGGC 
CTTTACAACC GAAGTGGAAG CCTTAGTGGA CGGAGCAGCC TCAAGGGGGA GGCAGAGAAT 
TCCACTGAGG TCAGCACCGT GCTCAAGCTG GACAACACTG TGGTGGGGCA AACAGCCTGG 
AAGCCATGCG GCCCCAATGC CTGGGACCAG AGCTTCACCC TGGAGCTGGA GAGGGCTCGG 
GAGCTGGAGT TGGCTGTGTT CTGGCGTGAC CAGAGGGGTC TGTGTGCTCT CAAATTTCTG 
AAGTTGGAAG ACTTCTTGGA CAATGAGAGG CATGAGGTGC AGCTGGACAT GGAACCCCAG 
GGCTGCCTGG TGGCTGAGGT CACCTTCCGT AACCCCATCA TCGAGCGGAT CCCTAGGCTC 
CAAAGGCAGA AAAAAATTTT CTCCAAGCAG CAAGGGCAGA CATTTCAGCG TGCCAGACAG 
ATGAACATCG ATGTGGCCAC CTGGGTGCGG CTGCTCCGGA GACTCATCCC GAACGCCGTG 
GCCACTGGCT CCTTCAGCCC CAATGCATCT CCAGGCTCTG AGATCCGGAG CACTGGAGAC 
ATATCCATGG AGAAATTGAA TCTCGGTGCT GACTCAGACA GCTCGTCCCA GAAGAGCCCC 
GCAGGGCTGC CCTCCACCTC ATGTAGCCTG AGTTCTCCAA CCCACGAATC CACCACCTCT 
CCAGAGCTGC CTTCAGAGAC CCAGGAGACC CCAGGCCCTG GCCTGTGCAG TCCCCTGAGG 
AAGTCGCCCC TGACGCTTGA GGACTTCAAG TTCCTGGCAG TGCTTGGTCG GGGTCACTTT 
GGAAAGGTGC TGCTGTCTGA ATTCCACTCC AGTGGGGAGC TCTTTGCCAT TAAAGCCTTG 
AAGAAAGGTG ACATTGTAGC CCGGGATGAG GTTGAGAGCC TGATGTGTGA GAAGCGGATT 
TTGGCGACCG TGACCAGGGC AGGACATCCC TTCCTGGTGA ACCTTTTCGG CTGTTTCCAG 
ACCCCAGAGC ATGTGTGCTT TGTGATGGAG TACTCAGCCG GTGGGGACTT GATGCTGCAT 
ATCCACAGCG ACGTGTTCTC AGAGCCTCGG GCTGTCTTCT ATTCGGCCTG TGTGGTGCTG 
GGACTGCAGT TCCTCCATGA ACACAAGATT GTCTACAGGG ACCTGAAGTT GGACAATTTG 
CTCCTGGATA CTGAGGGCTA CGTCAAGATC GCAGACTTTG GCCTCTGCAA GGAGGGGATG 
GGCTATGGGG ACCGGACCAG CACATTCTGC GGAACTCCGG AGTTCCTGGC GCCGGAAGTG 
CTCACAGACA CATCCTACAC TCGAGCCGTG GACTGGTGGG GACTGGGTGT ATTGCTCTAT 
GAGATGCTGG TTGGAGAGTC TCCGTTCCCT GGGGACGACG AGGAGGAAGT ATTTGACAGC 
ATCGTCAATG ATGAGGTTCG TTATCCCCGC TTCCTGTCTG CGGAGGCCAT CGGCATCATG 
AGAAGGCTAC TGCGGAGGAA CCCAGAGCGG AGGTTGGGAT CCACTGAGCG TGATGCAGAA 
GATGTGAAAA AACAGCCTTT CTTCAGGACT CTGGACTGGG ATGCCCTGCT GGCCCGTCGC 
CTGCCTCCAC CCTTCGTGCC TACACTTTCG GGGCGCACAG ACGTCAGCAA CTTCGATGAG 
GAGTTCACTG GGGAGGCCCC CACACTGAGC CCTCCCCGGG ATGCACGGCC CCTGACAGCT 
GCGGAGCAGG CGGCCTTCCG GGATTTCGAC TTTGTGGCAG GAGGCTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of Protein Kinase Novel 1 (PKN1) is associated with mild systolic and diastolic contractile dysfunction, increased phospholamban Thr17 phosphorylation, and exacerbated ischaemia-reperfusion injury.
Cardiovascular research114(1)138-157(2018 Jan)
Francois AA,Obasanjo-Blackshire K,Clark JE,Boguslavskyi A,Holt MR,Parker PJ,Marber MS,Heads RJ

Protein kinase N1, a cell inhibitor of Akt kinase, has a central role in quality control of germinal center formation.
Proceedings of the National Academy of Sciences of the United States of America109(51)21022-7(2012 Dec)
Yasui T,Sakakibara-Yada K,Nishimura T,Morita K,Tada S,Mosialos G,Kieff E,Kikutani H

Protein kinase N1 is a novel substrate of NFATc1-mediated cyclin D1-CDK6 activity and modulates vascular smooth muscle cell division and migration leading to inward blood vessel wall remodeling.
The Journal of biological chemistry287(43)36291-304(2012 Oct)
Singh NK,Kundumani-Sridharan V,Kumar S,Verma SK,Kotla S,Mukai H,Heckle MR,Rao GN

Regulatory domain selectivity in the cell-type specific PKN-dependence of cell migration.
PloS one6(7)e21732(2011)
Lachmann S,Jevons A,De Rycker M,Casamassima A,Radtke S,Collazos A,Parker PJ

Phosphorylation of histone H3T6 by PKCbeta(I) controls demethylation at histone H3K4.
Nature464(7289)792-6(2010 Apr)
Metzger E,Imhof A,Patel D,Kahl P,Hoffmeyer K,Friedrichs N,M?ller JM,Greschik H,Kirfel J,Ji S,Kunowska N,Beisenherz-Huss C,G?nther T,Buettner R,Sch?le R

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Pkn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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