gRNAs
The following Lrrc32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lrrc32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa06212 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001170434.1 , XM_006229747.3 | |
| Accession Version | NM_001170434.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-04-18 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | leucine-rich repeat-containing protein 32 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM029675.1 and AABR07004868.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: FM029675.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGAGCTACC AGATCCTGAT ACTCCTGGCC ATGCTGACCC TGGGCCTGGC TATCTCTCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001163905.1 |
| CDS | 62..2053 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001170434.1 |
1 | ATGAGCTACC AGATCCTGAT ACTCCTGGCC ATGCTGACCC TGGGCCTGGC TATCTCTCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa06212 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001170434.1 , XM_006229747.3 | |
| Accession Version | XM_006229747.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | leucine-rich repeat-containing protein 32 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCTACC AGATCCTGAT ACTCCTGGCC ATGCTGACCC TGGGCCTGGC TATCTCTCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006229809.1 |
| CDS | 920..2911 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006229747.3 |
1 | ATGAGCTACC AGATCCTGAT ACTCCTGGCC ATGCTGACCC TGGGCCTGGC TATCTCTCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa23127 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006229746.3 | |
| Accession Version | XM_006229746.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | leucine-rich repeat-containing protein 32 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229746.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGATGGGA CAGATTGTCC CGTACTGGAG GGAAGCAAGA ACACAAACAG TGGAGTTGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006229808.1 |
| CDS | 852..2924 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006229746.3 |
1 | ATGGATGGGA CAGATTGTCC CGTACTGGAG GGAAGCAAGA ACACAAACAG TGGAGTTGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Glycoprotein A repetitions predominant (GARP) positively regulates transforming growth factor (TGF) ?3 and is essential for mouse palatogenesis. |
GARP regulates the bioavailability and activation of TGF?. |
Membrane protein GARP is a receptor for latent TGF-beta on the surface of activated human Treg. |
GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells. |
Identification of a regulatory T cell specific cell surface molecule that mediates suppressive signals and induces Foxp3 expression. |
