Gpi cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpi
Entrez Gene ID	292804
Full Name	glucose-6-phosphate isomerase
Synonyms	Amf,Gpi1,Nlk,Pgi,Phi
General protein information	Preferred Names glucose-6-phosphate isomerase Names glucose-6-phosphate isomerase autocrine motility factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	This gene encodes a member of the glucose phosphate isomerase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. [provided by RefSeq, Jan 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_207592.1	NP_997475.1	glucose-6-phosphate isomerase

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPI	NP_000166.2
Rattus norvegicus (Norway rat)	Gpi	NP_997475.1
Drosophila melanogaster (fruit fly)	Pgi	NP_724724.1
Schizosaccharomyces pombe (fission yeast)	pgi1	NP_596635.1
Neurospora crassa	NCU07281	XP_962135.1
Arabidopsis thaliana (thale cress)	AT5G42740	NP_199088.1
Oryza sativa (rice)	Os06g0256500	NP_001057316.1
Xenopus tropicalis (tropical clawed frog)	gpi	NP_001011010.1
Mus musculus (house mouse)	Gpi1	NP_032181.2
Gallus gallus (chicken)	GPI	NP_001006128.1
Caenorhabditis elegans (roundworm)	gpi-1	NP_001021838.1
Kluyveromyces lactis	KLLA0E23519g	XP_455013.1
Eremothecium gossypii	AGOS_AEL249C	NP_984612.1
Magnaporthe oryzae (rice blast fungus)	MGG_12822	XP_003717069.1
Pan troglodytes (chimpanzee)	GPI	XP_003316332.1
Canis lupus familiaris (dog)	GPI	XP_853858.1
Danio rerio (zebrafish)	gpib	NP_658910.2
Oryza sativa (rice)	Os03g0776000	NP_001051434.1
Danio rerio (zebrafish)	gpia	NP_658909.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012167	XP_320366.3
Bos taurus (cattle)	GPI	NP_001035561.1
Saccharomyces cerevisiae (baker's yeast)	PGI1	NP_009755.1

Gene Ontology
Bibliography

Related articles in PubMed

Two novel mutations (p.(Ser160Pro) and p.(Arg472Cys)) causing glucose-6-phosphate isomerase deficiency are associated with erythroid dysplasia and inappropriately suppressed hepcidin.
Mojzikova R, Koralkova P, Holub D, Saxova Z, Pospisilova D, Prochazkova D, Dzubak P, Horvathova M, Divoky V
Blood cells, molecules & diseases(2017 Apr)

Survival of glucose phosphate isomerase null somatic cells and germ cells in adult mouse chimaeras.
Keighren MA, Flockhart JH, West JD
Biology open5(5)596-610(2016 May)

Neuroleukin/Autocrine Motility Factor Receptor Pathway Promotes Proliferation of Articular Chondrocytes through Activation of AKT and Smad2/3.
Tian K, Zhong W, Zheng X, Zhang J, Liu P, Zhang W, Liu H
Scientific reports515101(2015 Oct)

Polyubiquitylation of AMF requires cooperation between the gp78 and TRIM25 ubiquitin ligases.
Wang Y, Ha SW, Zhang T, Kho DH, Raz A, Xie Y
Oncotarget5(8)2044-51(2014 Apr)

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Neuroleukin/Autocrine Motility Factor Receptor Pathway Promotes Proliferation of Articular Chondrocytes through Activation of AKT and Smad2/3
Title: Neuroleukin/Autocrine Motility Factor Receptor Pathway Promotes Proliferation of Articular Chondrocytes through Activation of AKT and Smad2/3.

Our results show that glucose-6-phosphate is not the only substrate of Glucose-6-Phosphate Isomerase.
Title: A new role of glucose-6-phosphate isomerase: protection of cell structures from malonic dialdehyde.

induces hyperpermeability of endothelial and mesothelial cells leading to accumulation of ascites fluid (Tumor Autocrine Motility Factor)
Title: Tumor autocrine motility factor induces hyperpermeability of endothelial and mesothelial cells leading to accumulation of ascites fluid.

The following Gpi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13082	NM_207592.1 Latest version!	Rattus norvegicus glucose-6-phosphate isomerase (Gpi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpi ( NM_207592.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_997475.1 Rattus norvegicus glucose-6-phosphate isomerase (Gpi), mRNA.

Price & Availability↑

CloneID	ORa13082
Clone ID Related Accession (Same CDS sequence)	NM_207592.1
Accession Version	NM_207592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	glucose-6-phosphate isomerase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC062005.1. On Aug 30, 2004 this sequence version replaced XM_214908.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062005.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMN01906348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: inferred from conservation ##RefSeq-Attributes-END##

ATGGCTGCGC TCACCCGGAA CCCGGAGTTC CAGAAGCTGC TGGAGTGGCA CCGGGCGAAC 
TCTGCCAACC TCAAGCTGCG CGAACTTTTT GAGGCAGATC CGGAGCGCTT CAACCACTTC 
AGCTTGAACC TCAACACCAA CCATGGACAT ATTCTGTTGG ATTACTCCAA GAACCTTGTG 
AACAAGGAGG TGTTGCACAT GCTGGTGGAC CTGGCCAAGT CCAGAGGCGT GGAGGCCGCG 
CGGGACAACA TGTTCAGTGG TTTGAAGATC AACTCCACCG AGGACCGGGC GGTGCTGCAC 
GTGGCCCTTC GGAACCGGTC CAACAGATCC ATCATGATGG ACGGCAAAGA CGTGATGCCA 
GAGGTCAACA AGGTGCTGGA CAAGATGAAG TCTTTCTGCC AGCGGGTCCG GAGTGGTGAC 
TGGAAAGGGT ACACCGGCAA AGCTATCACG GACATCATCA ACATCGGCAT TGGAGGCTCC 
GACCTGGGAC CCCTCATGGT GACTGAAGCT CTCAAGCCTT ACTCTAAAGG AGGTCCCAGA 
GTCTGGTTTG TCTCCAACAT TGATGGGACC CACATTGCCA AAACATTGGC CAACCTGAAC 
CCTGAGTCTT CCCTCTTTAT AATCGCCTCC AAGACCTTCA CCACCCAGGA GACCATCACC 
AACGCAGAGA CTGCCAAAGA GTGGTTTCTC CAAGCTGCGA AGGATCCGTC TGCAGTTGCA 
AAGCACTTTG TTGCCCTGTC TACGAACACG GACAAAGTGA AGGAGTTTGG AATTGACCCT 
AAAAACATGT TCGAGTTCTG GGATTGGGTA GGTGGCCGCT ACTCGCTGTG GTCAGCCATT 
GGACTCTCCA TCGCACTGCA TGTGGGTTTT GACCACTTTG AACAGCTGCT GTCCGGGGCT 
CACTGGATGG ACCAGCACTT CATGAAGACG CCCCTGGATA AGAATGCCCC CGTCCTGCTG 
GCTCTCCTGG GCATCTGGTA TATCAACTTC TACGGCTGTG AGACCCACGC CATGCTGCCC 
TATGACCAGT ACATGCACCG CTTTGCTGCC TATTTCCAGC AGGGTGACAT GGAATCCAAT 
GGAAAGTACA TCACCAAGTC TGGAGCCCGG GTTGACTACC AGACAGGCCC CATTGTGTGG 
GGGGAGCCAG GGACCAACGG TCAACATGCA TTCTACCAGC TTATCCACCA AGGCACCAAG 
ATGATACCCT GTGATTTCCT CATCCCTGTT CAGACCCAGC ACCCCATACG GAATGGTCTG 
CATCACAAGA TCCTCCTGGC CAACTTCTTG GCCCAGACTG AGGCCCTGAT GAAGGGGAAG 
TCGCCAGAAG AGGCCAGGAA GGAGCTCCAG GCTGCCGGAA AGAGCCCAGA AGAACTGGAG 
AAACTCTTGC CACACAAGGT CTTTGAAGGA AACCGGCCGA CCAACTCAAT TGTGTTTACC 
AAGCTAACAC CCTTCATTCT GGGAGCACTG ATTGCCATGT ATGAGCACAA GATCTTCGTT 
CAGGGCATCA TCTGGGACAT CAACAGCTTC GACCAGTGGG GAGTGGAGCT GGGGAAGCAG 
CTGGCCAAGA AAATTGAGCC AGAGCTGGAC GGCAGCTCTG CCGTAACCTC CCATGACTCC 
TCCACTAATG GACTGATCGG CTTCATCAAG CTGCAGCGCG ACACCAAAAT AGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997475.1
CDS	67..1743
Translation	MAALTRNPEFQKLLEWHRANSANLKLRELFEADPERFNHFSLNLNTNHGHILLDYSKNLVNKEVLHMLVDLAKSRGVEAARDNMFSGLKINSTEDRAVLHVALRNRSNRSIMMDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKAITDIINIGIGGSDLGPLMVTEALKPYSKGGPRVWFVSNIDGTHIAKTLANLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTDKVKEFGIDPKNMFEFWDWVGGRYSLWSAIGLSIALHVGFDHFEQLLSGAHWMDQHFMKTPLDKNAPVLLALLGIWYINFYGCETHAMLPYDQYMHRFAAYFQQGDMESNGKYITKSGARVDYQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRNGLHHKILLANFLAQTEALMKGKSPEEARKELQAAGKSPEELEKLLPHKVFEGNRPTNSIVFTKLTPFILGALIAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSSAVTSHDSSTNGLIGFIKLQRDTKID

Target ORF information:

RefSeq Version	NM_207592.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucose-6-phosphate isomerase (Gpi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207592.1

ORF Insert Sequence:

ATGGCTGCGC TCACCCGGAA CCCGGAGTTC CAGAAGCTGC TGGAGTGGCA CCGGGCGAAC 
TCTGCCAACC TCAAGCTGCG CGAACTTTTT GAGGCAGATC CGGAGCGCTT CAACCACTTC 
AGCTTGAACC TCAACACCAA CCATGGACAT ATTCTGTTGG ATTACTCCAA GAACCTTGTG 
AACAAGGAGG TGTTGCACAT GCTGGTGGAC CTGGCCAAGT CCAGAGGCGT GGAGGCCGCG 
CGGGACAACA TGTTCAGTGG TTTGAAGATC AACTCCACCG AGGACCGGGC GGTGCTGCAC 
GTGGCCCTTC GGAACCGGTC CAACAGATCC ATCATGATGG ACGGCAAAGA CGTGATGCCA 
GAGGTCAACA AGGTGCTGGA CAAGATGAAG TCTTTCTGCC AGCGGGTCCG GAGTGGTGAC 
TGGAAAGGGT ACACCGGCAA AGCTATCACG GACATCATCA ACATCGGCAT TGGAGGCTCC 
GACCTGGGAC CCCTCATGGT GACTGAAGCT CTCAAGCCTT ACTCTAAAGG AGGTCCCAGA 
GTCTGGTTTG TCTCCAACAT TGATGGGACC CACATTGCCA AAACATTGGC CAACCTGAAC 
CCTGAGTCTT CCCTCTTTAT AATCGCCTCC AAGACCTTCA CCACCCAGGA GACCATCACC 
AACGCAGAGA CTGCCAAAGA GTGGTTTCTC CAAGCTGCGA AGGATCCGTC TGCAGTTGCA 
AAGCACTTTG TTGCCCTGTC TACGAACACG GACAAAGTGA AGGAGTTTGG AATTGACCCT 
AAAAACATGT TCGAGTTCTG GGATTGGGTA GGTGGCCGCT ACTCGCTGTG GTCAGCCATT 
GGACTCTCCA TCGCACTGCA TGTGGGTTTT GACCACTTTG AACAGCTGCT GTCCGGGGCT 
CACTGGATGG ACCAGCACTT CATGAAGACG CCCCTGGATA AGAATGCCCC CGTCCTGCTG 
GCTCTCCTGG GCATCTGGTA TATCAACTTC TACGGCTGTG AGACCCACGC CATGCTGCCC 
TATGACCAGT ACATGCACCG CTTTGCTGCC TATTTCCAGC AGGGTGACAT GGAATCCAAT 
GGAAAGTACA TCACCAAGTC TGGAGCCCGG GTTGACTACC AGACAGGCCC CATTGTGTGG 
GGGGAGCCAG GGACCAACGG TCAACATGCA TTCTACCAGC TTATCCACCA AGGCACCAAG 
ATGATACCCT GTGATTTCCT CATCCCTGTT CAGACCCAGC ACCCCATACG GAATGGTCTG 
CATCACAAGA TCCTCCTGGC CAACTTCTTG GCCCAGACTG AGGCCCTGAT GAAGGGGAAG 
TCGCCAGAAG AGGCCAGGAA GGAGCTCCAG GCTGCCGGAA AGAGCCCAGA AGAACTGGAG 
AAACTCTTGC CACACAAGGT CTTTGAAGGA AACCGGCCGA CCAACTCAAT TGTGTTTACC 
AAGCTAACAC CCTTCATTCT GGGAGCACTG ATTGCCATGT ATGAGCACAA GATCTTCGTT 
CAGGGCATCA TCTGGGACAT CAACAGCTTC GACCAGTGGG GAGTGGAGCT GGGGAAGCAG 
CTGGCCAAGA AAATTGAGCC AGAGCTGGAC GGCAGCTCTG CCGTAACCTC CCATGACTCC 
TCCACTAATG GACTGATCGG CTTCATCAAG CTGCAGCGCG ACACCAAAAT AGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Two novel mutations (p.(Ser160Pro) and p.(Arg472Cys)) causing glucose-6-phosphate isomerase deficiency are associated with erythroid dysplasia and inappropriately suppressed hepcidin.
Blood cells, molecules & diseases(2017 Apr)
Mojzikova R,Koralkova P,Holub D,Saxova Z,Pospisilova D,Prochazkova D,Dzubak P,Horvathova M,Divoky V

Survival of glucose phosphate isomerase null somatic cells and germ cells in adult mouse chimaeras.
Biology open5(5)596-610(2016 May)
Keighren MA,Flockhart JH,West JD

Neuroleukin/Autocrine Motility Factor Receptor Pathway Promotes Proliferation of Articular Chondrocytes through Activation of AKT and Smad2/3.
Scientific reports515101(2015 Oct)
Tian K,Zhong W,Zheng X,Zhang J,Liu P,Zhang W,Liu H

Polyubiquitylation of AMF requires cooperation between the gp78 and TRIM25 ubiquitin ligases.
Oncotarget5(8)2044-51(2014 Apr)
Wang Y,Ha SW,Zhang T,Kho DH,Raz A,Xie Y

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

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Gpi cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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