Slc1a5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc1a5
Entrez Gene ID	292657
Full Name	solute carrier family 1 member 5
Synonyms	Asct2,H4-ASCT2,Slc1a7
General protein information	Preferred Names solute carrier family 1 member 5 Names neutral amino acid transporter B(0) CAZ-associated structural protein H4-system ASC-like transporter sodium-dependent neutral amino acid transporter ASCT2 solute carrier family 1 (neutral amino acid transporter), member 5 solute carrier family 1, member 7
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	glutamine transporter; involved in glutamine efflux from astrocytes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_175758.3	NP_786934.1	neutral amino acid transporter B(0)

Pathways from BioSystems

Homologies

	SLC1A5	XP_002801359.1
Bos taurus (cattle)	SLC1A5	NP_777026.1
Pan troglodytes (chimpanzee)	SLC1A5	XP_512773.2
Canis lupus familiaris (dog)	SLC1A5	XP_541540.2
Mus musculus (house mouse)	Slc1a5	NP_033227.2
Danio rerio (zebrafish)	slc1a5	NP_001177684.1
Danio rerio (zebrafish)	LOC100535841	XP_003200064.1
Homo sapiens (human)	SLC1A5	NP_005619.1
Rattus norvegicus (Norway rat)	Slc1a5	NP_786934.1
Xenopus tropicalis (tropical clawed frog)	slc1a5	NP_001008127.1

Gene Ontology
Bibliography

Related articles in PubMed

D-Serine Is a Substrate for Neutral Amino Acid Transporters ASCT1/SLC1A4 and ASCT2/SLC1A5, and Is Transported by Both Subtypes in Rat Hippocampal Astrocyte Cultures.
Foster AC, Farnsworth J, Lind GE, Li YX, Yang JY, Dang V, Penjwini M, Viswanath V, Staubli U, Kavanaugh MP
PloS one11(6)e0156551(2016)

Expression of Glutamate and Glutamine Transporter Proteins in Neurovascular Unit Cells In Vitro.
Morgun AV, Kuvacheva NV, Khilazheva ED, Taranushenko TE, Salmina AB
Bulletin of experimental biology and medicine159(5)614-6(2015 Sep)

In vivo D-serine hetero-exchange through alanine-serine-cysteine (ASC) transporters detected by microelectrode biosensors.
Maucler C, Pernot P, Vasylieva N, Pollegioni L, Marinesco S
ACS chemical neuroscience4(5)772-81(2013 May)

A novel human endogenous retroviral protein inhibits cell-cell fusion.
Sugimoto J, Sugimoto M, Bernstein H, Jinno Y, Schust D
Scientific reports31462(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the selectivity profile of D-serine uptake in cultured rat hippocampal astrocytes is consistent with uptake mediated by both ASCT1 and ASCT2. Together these data suggest that SLC1A4 (ASCT1) may represent an important route of Na-dependent D-serine flux in the brain that has the ability to regulate extracellular D-serine and thereby NMDA receptor activity.
Title: D-Serine Is a Substrate for Neutral Amino Acid Transporters ASCT1/SLC1A4 and ASCT2/SLC1A5, and Is Transported by Both Subtypes in Rat Hippocampal Astrocyte Cultures.

Hypoxic exposure significantly increased SLC1A5 expression in neurovascular unit cells in vitro, but suppressed EAAT2 expression.
Title: Expression of Glutamate and Glutamine Transporter Proteins in Neurovascular Unit Cells In Vitro.

D-serine heteroexchange through ASC transporters, in particular low affinity ASCT2, is present in vivo and may constitute a key component in the regulation of D-serine extracellular concentration.
Title: In vivo D-serine hetero-exchange through alanine-serine-cysteine (ASC) transporters detected by microelectrode biosensors.

Basic amino acid transporter is localized in a punctuate manner following phorbol myristate acetate treatment when more pronounced bigger puncta are visible, indicating formation of bigger plasma membrane microdomains or astrocyte intracellular vesicles.
Title: Regulation of amino acid/carnitine transporter B 0,+ (ATB 0,+) in astrocytes by protein kinase C: independent effects on raft and non-raft transporter subpopulations.

The data demonstrated that the inhibition of ASCT2 by mercury compounds was due to covalent reactions with Cys residue(s) of the transporter.
Title: Inactivation by Hg2+ and methylmercury of the glutamine/amino acid transporter (ASCT2) reconstituted in liposomes: Prediction of the involvement of a CXXC motif by homology modelling.

The following Slc1a5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc1a5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03936	NM_175758.3 Latest version!	Rattus norvegicus solute carrier family 1 member 5 (Slc1a5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc1a5 ( NM_175758.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_786934.1 Rattus norvegicus solute carrier family 1 member 5 (Slc1a5), mRNA.

Price & Availability↑

CloneID	ORa03936
Clone ID Related Accession (Same CDS sequence)	NM_175758.3
Accession Version	NM_175758.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	neutral amino acid transporter B(0)
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC080242.1. On or before Oct 20, 2005 this sequence version replaced NM_153723.1, NM_175758.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC080242.1, AJ132846.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGTGG ATCCTCCTAA GGCTGACCCC AAAGGGGTGG TGGCGGTGGA TCCCACCGCG 
AACTGTGGCT CGGGGCTGAA GTCCAGAGAG GACCAGGGAG CGAAAGCAGG TGGATGCTGC 
AGTTCCCGGG ACCAAGTGTG CCGCTGCCTT CGCGCCAACC TGCTGGTTCT GCTCACCGTG 
GCGGCGGCGG TGGCTGGCGT GGTGCTAGGT CTGGGGGTCT CGGCGGCGGG CGGTGCTGAG 
GCGCTGGGCC ACGCGCGCTT TACCGCTTTC GCCTTCCCGG GAGAGCTGCT GTTGCGTCTG 
CTGGAGATGA TCATCCTGCC GCTGGTGGTG TGCAGCCTGA TCGGAGGTGC AGCCAGTCTG 
GACCCTAGCG CGCTCGGCCG TTTGGGCGCC TGGGCCCTGC TCTTTTTCCT GGTCACCACA 
CTGCTCTCTT CGGCTCTCGG CGTGGCCTTG GCCCTGGCGC TGAAGCCGGG CGCCGCGTTT 
GCTGCCATCA ACTCCTCTGT TGTAGACTCC AGTGTCCACA GAGCACCCAC CAAAGAGGTG 
CTGGATTCCT TTCTGGAACT CCTGAGGAAT ATGTTCCCCT CCAATCTGGT GTCTGCTTCT 
GCTGCCTTCC GCATTCCATG TGGTGCCTGT CCACAGAGGA GCAATGCAAC CATGGACCAG 
CCTCACTGTG AGATGAAGAT GAACATTCTG GGCTTGGTCG TGTTCGCTAT AGTCTTTGGC 
GTGGCTCTGA GGAAGCTGGG GCCCGAGGGT GAGCTGCTCA TCCGATTCTT CAACTCCTTC 
AATGATGCCA CCATGGTCCT GGTCTCCTGG ATTATGTGGT ACGCCCCCAT TGGCATCTTG 
TTCCTGGTGG CCGGCAAGAT TGTGGAGATG AAAGACATCC GCCAGCTCTT CATCGGCCTC 
GGCAAATACA TCGTGTGCTG CCTGCTGGGC CACGCCATCC ACGGGCTCCT GGTTCTGCCT 
CTCATCTACT TCCTCTTTAC CCGCAAAAAC CCTTATCGAT TCCTGTGGGG CATCGTGACA 
CCCCTGGCTA CAGCTTTCGG GACCTCTTCT AGCTCTGCCA CATTGCCCCT GATGATGAAG 
TGTGTAGAGG AGAAGAATGG TGTGGCCAAA CACATCAGCC GGTTCATCCT GCCCATCGGC 
GCCACCGTCA ATATGGACGG GGCAGCGTTG TTCCAGTGCG TGGCGGCAGT GTTCATTGCA 
CAACTAAATG GGATGTCCCT GGACTTCGTG AAGATCATCA CCATCCTGGT CACAGCTACT 
GCATCCAGTG TCGGGGCCGC AGGTATCCCT GCAGGGGGCG TCCTCACTCT TGCCATCATT 
CTAGAAGCAA TCAGCCTCCC TGTCAAGGAC ATCTCCTTGA TCTTGGCCGT GGACTGGCTA 
GTGGACAGGT CCTGTACGGT CCTCAACGTG GAGGGTGATG CTTTTGGGGC CGGATTGCTC 
CAGAGTTACG TGGATCGCAC CAAGATGCCG AGCTCAGAGC CTGAACTGAT CCAGGTGAAG 
AACGACGTGT CTCTGAAACC ATTGCCCCTC GCCACAGAGG AGGGGAACCC CCTTCTGAAA 
CAGTGCCGGG AACCCTCCGG GGACTCCAGT GCCACATGCG AAAAGGAATC TGTCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_786934.1
CDS	392..2011
Translation	MAVDPPKADPKGVVAVDPTANCGSGLKSREDQGAKAGGCCSSRDQVCRCLRANLLVLLTVAAAVAGVVLGLGVSAAGGAEALGHARFTAFAFPGELLLRLLEMIILPLVVCSLIGGAASLDPSALGRLGAWALLFFLVTTLLSSALGVALALALKPGAAFAAINSSVVDSSVHRAPTKEVLDSFLELLRNMFPSNLVSASAAFRIPCGACPQRSNATMDQPHCEMKMNILGLVVFAIVFGVALRKLGPEGELLIRFFNSFNDATMVLVSWIMWYAPIGILFLVAGKIVEMKDIRQLFIGLGKYIVCCLLGHAIHGLLVLPLIYFLFTRKNPYRFLWGIVTPLATAFGTSSSSATLPLMMKCVEEKNGVAKHISRFILPIGATVNMDGAALFQCVAAVFIAQLNGMSLDFVKIITILVTATASSVGAAGIPAGGVLTLAIILEAISLPVKDISLILAVDWLVDRSCTVLNVEGDAFGAGLLQSYVDRTKMPSSEPELIQVKNDVSLKPLPLATEEGNPLLKQCREPSGDSSATCEKESVM

Target ORF information:

RefSeq Version	NM_175758.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 1 member 5 (Slc1a5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175758.3

ORF Insert Sequence:

ATGGCAGTGG ATCCTCCTAA GGCTGACCCC AAAGGGGTGG TGGCGGTGGA TCCCACCGCG 
AACTGTGGCT CGGGGCTGAA GTCCAGAGAG GACCAGGGAG CGAAAGCAGG TGGATGCTGC 
AGTTCCCGGG ACCAAGTGTG CCGCTGCCTT CGCGCCAACC TGCTGGTTCT GCTCACCGTG 
GCGGCGGCGG TGGCTGGCGT GGTGCTAGGT CTGGGGGTCT CGGCGGCGGG CGGTGCTGAG 
GCGCTGGGCC ACGCGCGCTT TACCGCTTTC GCCTTCCCGG GAGAGCTGCT GTTGCGTCTG 
CTGGAGATGA TCATCCTGCC GCTGGTGGTG TGCAGCCTGA TCGGAGGTGC AGCCAGTCTG 
GACCCTAGCG CGCTCGGCCG TTTGGGCGCC TGGGCCCTGC TCTTTTTCCT GGTCACCACA 
CTGCTCTCTT CGGCTCTCGG CGTGGCCTTG GCCCTGGCGC TGAAGCCGGG CGCCGCGTTT 
GCTGCCATCA ACTCCTCTGT TGTAGACTCC AGTGTCCACA GAGCACCCAC CAAAGAGGTG 
CTGGATTCCT TTCTGGAACT CCTGAGGAAT ATGTTCCCCT CCAATCTGGT GTCTGCTTCT 
GCTGCCTTCC GCATTCCATG TGGTGCCTGT CCACAGAGGA GCAATGCAAC CATGGACCAG 
CCTCACTGTG AGATGAAGAT GAACATTCTG GGCTTGGTCG TGTTCGCTAT AGTCTTTGGC 
GTGGCTCTGA GGAAGCTGGG GCCCGAGGGT GAGCTGCTCA TCCGATTCTT CAACTCCTTC 
AATGATGCCA CCATGGTCCT GGTCTCCTGG ATTATGTGGT ACGCCCCCAT TGGCATCTTG 
TTCCTGGTGG CCGGCAAGAT TGTGGAGATG AAAGACATCC GCCAGCTCTT CATCGGCCTC 
GGCAAATACA TCGTGTGCTG CCTGCTGGGC CACGCCATCC ACGGGCTCCT GGTTCTGCCT 
CTCATCTACT TCCTCTTTAC CCGCAAAAAC CCTTATCGAT TCCTGTGGGG CATCGTGACA 
CCCCTGGCTA CAGCTTTCGG GACCTCTTCT AGCTCTGCCA CATTGCCCCT GATGATGAAG 
TGTGTAGAGG AGAAGAATGG TGTGGCCAAA CACATCAGCC GGTTCATCCT GCCCATCGGC 
GCCACCGTCA ATATGGACGG GGCAGCGTTG TTCCAGTGCG TGGCGGCAGT GTTCATTGCA 
CAACTAAATG GGATGTCCCT GGACTTCGTG AAGATCATCA CCATCCTGGT CACAGCTACT 
GCATCCAGTG TCGGGGCCGC AGGTATCCCT GCAGGGGGCG TCCTCACTCT TGCCATCATT 
CTAGAAGCAA TCAGCCTCCC TGTCAAGGAC ATCTCCTTGA TCTTGGCCGT GGACTGGCTA 
GTGGACAGGT CCTGTACGGT CCTCAACGTG GAGGGTGATG CTTTTGGGGC CGGATTGCTC 
CAGAGTTACG TGGATCGCAC CAAGATGCCG AGCTCAGAGC CTGAACTGAT CCAGGTGAAG 
AACGACGTGT CTCTGAAACC ATTGCCCCTC GCCACAGAGG AGGGGAACCC CCTTCTGAAA 
CAGTGCCGGG AACCCTCCGG GGACTCCAGT GCCACATGCG AAAAGGAATC TGTCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

D-Serine Is a Substrate for Neutral Amino Acid Transporters ASCT1/SLC1A4 and ASCT2/SLC1A5, and Is Transported by Both Subtypes in Rat Hippocampal Astrocyte Cultures.
PloS one11(6)e0156551(2016)
Foster AC,Farnsworth J,Lind GE,Li YX,Yang JY,Dang V,Penjwini M,Viswanath V,Staubli U,Kavanaugh MP

Expression of Glutamate and Glutamine Transporter Proteins in Neurovascular Unit Cells In Vitro.
Bulletin of experimental biology and medicine159(5)614-6(2015 Sep)
Morgun AV,Kuvacheva NV,Khilazheva ED,Taranushenko TE,Salmina AB

In vivo D-serine hetero-exchange through alanine-serine-cysteine (ASC) transporters detected by microelectrode biosensors.
ACS chemical neuroscience4(5)772-81(2013 May)
Maucler C,Pernot P,Vasylieva N,Pollegioni L,Marinesco S

A novel human endogenous retroviral protein inhibits cell-cell fusion.
Scientific reports31462(2013)
Sugimoto J,Sugimoto M,Bernstein H,Jinno Y,Schust D

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Slc1a5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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