Clcn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clcn2
Entrez Gene ID	29232
Full Name	chloride channel, voltage-sensitive 2
Synonyms	ClC-2
General protein information	Preferred Names chloride channel, voltage-sensitive 2 Names chloride channel protein 2 chloride channel 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23
Summary	has voltage-gated chloride channel activity; activity is also regulated by volume and pH; may play a role in chloride transport in many tissues [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017137.1	NP_058833.1	chloride channel protein 2

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	clh-4	NP_001024885.1
Pan troglodytes (chimpanzee)	CLCN2	XP_003950294.1
Rattus norvegicus (Norway rat)	LOC100912455	XP_003751129.1
Caenorhabditis elegans (roundworm)	clh-3	NP_001022060.1
Canis lupus familiaris (dog)	CLCN2	XP_005639832.1
Bos taurus (cattle)	CLCN2	NP_001095957.1
Rattus norvegicus (Norway rat)	Clcn2	NP_058833.1
Xenopus tropicalis (tropical clawed frog)	clcn2	XP_004914466.1
	CLCN2	XP_002808378.1
Mus musculus (house mouse)	Clcn2	NP_034030.2
Danio rerio (zebrafish)	LOC100333644	XP_002667721.3
Drosophila melanogaster (fruit fly)	ClC-a	NP_001097752.1
Homo sapiens (human)	CLCN2	NP_004357.3
Gallus gallus (chicken)	CLCN2	XP_423073.4
Anopheles gambiae (African malaria mosquito)	GPRMGL4	XP_003436287.1

Gene Ontology
Bibliography

Related articles in PubMed

Activated ClC-2 Inhibits p-Akt to Repress Myelination in GDM Newborn Rats.
He F, Peng Y, Yang Z, Ge Z, Tian Y, Ma T, Li H
International journal of biological sciences13(2)179-188(2017)

ClC-2 contributes to tonic inhibition mediated by ?5 subunit-containing GABA(A) receptor in experimental temporal lobe epilepsy.
Ge YX, Liu Y, Tang HY, Liu XG, Wang X
Neuroscience186120-7(2011 Jul)

Mouse model resources for vision research.
Won J, Shi LY, Hicks W, Wang J, Hurd R, Naggert JK, Chang B, Nishina PM
Journal of ophthalmology2011391384(2011)

Regulation of fast-spiking basket cell synapses by the chloride channel ClC-2.
F?ldy C, Lee SH, Morgan RJ, Soltesz I
Nature neuroscience13(9)1047-9(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ClC-2 and caspase-3 were induced by GDM, which resulted in apoptosis and myelination inhibition. The effect was caused by repressing PI3K-Akt signaling pathway. Application of ClC-2 inhibitor DIDS showed protective effects on cerebral white matter damage stimulated by high glucose concentration.
Title: Activated ClC-2 Inhibits p-Akt to Repress Myelination in GDM Newborn Rats.

ClC-2 is upregulated in pyramidal cells of pilocarpine-treated rats; this involves alpha5 subunit-containing GABA(A) receptor.
Title: ClC-2 contributes to tonic inhibition mediated by ?5 subunit-containing GABA(A) receptor in experimental temporal lobe epilepsy.

GABAA receptor-mediated synaptic inputs from basket cells were modulated by ClC-2. Our data reveal a previously unknown cell type-specific regulation of intracellular chloride homeostasis in the perisomatic region of hippocampal pyramidal neurons.
Title: Regulation of fast-spiking basket cell synapses by the chloride channel ClC-2.

functional ClC-2-type Cl(-) channel is expressed in rat rectal surface epithelium, but channel activity may be negligible & thus nonessential for controlling electrogenic Na(+) transport in this surface epithelium under basal physiological conditions.
Title: Functional characterization of a ClC-2-like Cl(-) conductance in surface epithelial cells of rat rectal colon.

the cholesterol environment regulates ClC-2 activity; ClC-2 activity is increased in response to acute oxidative or metabolic stress involving relocalization of this channel to membrane microdomains
Title: Membrane cholesterol content modulates ClC-2 gating and sensitivity to oxidative stress.

The following Clcn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clcn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa34409	NM_017137.1 Latest version!	Rattus norvegicus chloride channel, voltage-sensitive 2 (Clcn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clcn2 ( NM_017137.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058833.1 Rattus norvegicus chloride channel, voltage-sensitive 2 (Clcn2), mRNA.

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CloneID	ORa34409
Clone ID Related Accession (Same CDS sequence)	NM_017137.1
Accession Version	NM_017137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	chloride channel protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X64139.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X64139.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCG CAACGGCCGC GGCGGCGACG GTGGCGGGGG AAGGGATGGA GCCTCGAGCG 
CTGCAGTACG AGCAGACCCT GATGTATGGC CGGTATACTC AGGAACTCGG GGCCTTTGCC 
AAAGAGGAAG CTGCTCGGAT TCGCCTGGGA GGGCCTGAGC CCTGGAAGGG GTCCCCTTCT 
GCCCGGGCTA CCCCAGAGCT CCTAGAATAT GGACAGAGCC GATGTGCCAG ATGTCGCATT 
TGTTCTGTAC GCTGCCACAA GTTCCTAGTG TCCAGGGTCG GTGAAGATTG GATCTTCCTG 
GTCCTGTTGG GGCTCCTCAT GGCATTGGTC AGTTGGGCCA TGGACTATGC CATCGCTGTC 
TGTCTACAGG CTCAGCAATG GATGTCCCGG GGCTTAAACA CCAACATCTT ACTCCAGTAC 
CTGGCTTGGG TTACCTACCC TGTCGTCCTC ATCACTTTCT CTGCCGGATT CACCCAGATC 
CTGGCCCCGC AGGCTGTTGG GTCTGGCATC CCCGAGATGA AAACCATCCT TCGGGGAGTG 
GTGCTGAAAG AGTACCTCAC CCTCAAGACC TTTGTAGCTA AGGTCATCGG GCTGACCTGT 
GCCCTGGGCA GTGGGATGCC CCTTGGCAAG GAGGGACCCT TTGTGCACAT TGCCAGCATG 
TGTGCTGCCC TTCTCAGCAA GTTCCTCTCC CTCTTTGGGG GTATCTATGA GAACGAGTCC 
CGGAACACAG AGATGCTAGC TGCCGCATGC GCAGTAGGAG TGGGCTGCTG CTTTGCCGCA 
CCAATCGGAG GGGTCCTATT CAGCATTGAG GTCACCTCCA CCTTCTTCGC TGTTAGGAAC 
TACTGGCGGG GCTTCTTTGC GGCCACCTTC AGTGCCTTCA TCTTTCGGGT CTTGGCAGTG 
TGGAACCGTG ATGAAGAAAC CATCACAGCT CTCTTCAAAA CTCGGTTCCG ACTCGACTTC 
CCCTTTGACC TGCAGGAGCT GCCAGCTTTT GCTGTCATTG GTATTGCTAG TGGCTTCGGG 
GGAGCCCTCT TTGTCTACCT GAACCGGAAG ATTGTCCAGG TGATGCGGAA GCAAAAAACC 
ATCAACCGCT TCCTCATGAA GAAACGGCTG CTCTTCCCCG CACTGGTGAC TCTGCTCATC 
TCCACTCTGA CCTTCCCCCC TGGCTTTGGA CAGTTCATGG CCGGACAGCT CTCACAGAAA 
GAGACCCTGG TCACACTGTT TGACAACCGG ACATGGGTCC GCCAGGGCCT GGTAGAGGAT 
CTAGGAGCAC CTAGCACTTC ACAGGCCTGG AGCCCACCGC GCGCCAACGT CTTCCTGACT 
CTCGTCATCT TCATCCTCAT GAAGTTCTGG ATGTCTGCAC TGGCCACCAC CATCCCAGTG 
CCCTGTGGTG CCTTCATGCC TGTCTTTGTC ATTGGAGCAG CATTTGGCCG GCTGGTGGGC 
GAGAGCATGG CTGCCTGGTT CCCAGATGGG ATTCACACGG ATAGCAGTAC CTACCGGATT 
GTACCTGGAG GCTATGCCGT GGTCGGGGCA GCTGCACTTG CAGGAGCAGT GACACACACA 
GTGTCCACAG CAGTGATTGT CTTCGAGCTC ACCGGCCAGA TTGCCCACAT TCTGCCCGTC 
ATGATTGCTG TCATCCTGGC GAATGCCGTT GCCCAGAGCC TGCAGCCCTC CCTCTATGAC 
AGTATCATCC GCATCAAGAA GCTGCCCTAC CTGCCTGAGC TGGGCTGGGG CCGCCACCAG 
CAGTACCGGG TACGAGTAGA GGACATCATG GTACGGGATG TACCCCATGT AGCCCTCAGC 
TGCACTTTTC GGGACCTGCG ACTGGCACTG CACCGAACCA AGGGCCGTAT GTTGGCCCTA 
GTGGAGTCAC CTGAGTCCAT GATTCTGCTG GGCTCCATCG AACGCTCACA GGTGGTAGCG 
CTACTGGGAG CACAGCTGAG TCCAGCGCGC CGGCGGCAGC ACATGCAAAA GCTAAGGAAA 
GCCCAGATGT CCCCACCGTC AGATCAGGAG AGCCCCCCCA GCTCTGAGAC GTCCATCCGC 
TTCCAGGTGA ACACAGAGGA CTCAGGCTTC CCTGGAGCCC ATGGGCAGAC TCACAAGCCC 
CTGAAGCCTG CTCTAAAGAG AGGGCCCAGC AACGCCACAA GCCTCCAGGA AGGTACCACG 
GGCAACATGG AATCAGCAGG CATTGCCCTC AGAAGCCTCT TCTGTGGCAG TCCACCTCTG 
GAGTCGACGA CGTCAGAATT GGAAAAGTCG GAATCCTGTG ACAAACGCAA GCTGAAGCGG 
GTCCGAATCT CCCTGGCGAG TGACTCAGAC CTGGAAGGCA AGATGAGTCC CGAGGAGATC 
TTAGAGTGGG AAGAGCAGCA GCTAGATGAG CCAGTCAACT TCAGTGACTG CAAAATCGAC 
CCCGCCCCCT TCCAGCTAGT GGAGCGGACT TCTTTGCACA AGACCCACAC CATCTTCTCG 
CTGCTGGGAG TGGACCATGC TTATGTCACC AGCATTGGCA GACTCATTGG GATTGTCACC 
CTAAAGGAGC TCCGGAAGGC CATTGAAGGC TCTGTCACAG CACAGGGTGT AAAAGTCAGG 
CCACCCCTTG CCAGTTTCCG GGACAGTGCC ACCAGCAGCA GTGACACAGA GACCACCGAG 
GTGCATGCAC TCTGGGGGCC GAGATCCCGC CACGGCCTCC CACGAGAGGG TACCCCCTCT 
GATAGTGATG ACAAGTGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058833.1
CDS	169..2892
Translation	MAAATAAAATVAGEGMEPRALQYEQTLMYGRYTQELGAFAKEEAARIRLGGPEPWKGSPSARATPELLEYGQSRCARCRICSVRCHKFLVSRVGEDWIFLVLLGLLMALVSWAMDYAIAVCLQAQQWMSRGLNTNILLQYLAWVTYPVVLITFSAGFTQILAPQAVGSGIPEMKTILRGVVLKEYLTLKTFVAKVIGLTCALGSGMPLGKEGPFVHIASMCAALLSKFLSLFGGIYENESRNTEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRKIVQVMRKQKTINRFLMKKRLLFPALVTLLISTLTFPPGFGQFMAGQLSQKETLVTLFDNRTWVRQGLVEDLGAPSTSQAWSPPRANVFLTLVIFILMKFWMSALATTIPVPCGAFMPVFVIGAAFGRLVGESMAAWFPDGIHTDSSTYRIVPGGYAVVGAAALAGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQKLRKAQMSPPSDQESPPSSETSIRFQVNTEDSGFPGAHGQTHKPLKPALKRGPSNATSLQEGTTGNMESAGIALRSLFCGSPPLESTTSELEKSESCDKRKLKRVRISLASDSDLEGKMSPEEILEWEEQQLDEPVNFSDCKIDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPRSRHGLPREGTPSDSDDKCQ

Target ORF information:

RefSeq Version	NM_017137.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chloride channel, voltage-sensitive 2 (Clcn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017137.1

ORF Insert Sequence:

ATGGCGGCCG CAACGGCCGC GGCGGCGACG GTGGCGGGGG AAGGGATGGA GCCTCGAGCG 
CTGCAGTACG AGCAGACCCT GATGTATGGC CGGTATACTC AGGAACTCGG GGCCTTTGCC 
AAAGAGGAAG CTGCTCGGAT TCGCCTGGGA GGGCCTGAGC CCTGGAAGGG GTCCCCTTCT 
GCCCGGGCTA CCCCAGAGCT CCTAGAATAT GGACAGAGCC GATGTGCCAG ATGTCGCATT 
TGTTCTGTAC GCTGCCACAA GTTCCTAGTG TCCAGGGTCG GTGAAGATTG GATCTTCCTG 
GTCCTGTTGG GGCTCCTCAT GGCATTGGTC AGTTGGGCCA TGGACTATGC CATCGCTGTC 
TGTCTACAGG CTCAGCAATG GATGTCCCGG GGCTTAAACA CCAACATCTT ACTCCAGTAC 
CTGGCTTGGG TTACCTACCC TGTCGTCCTC ATCACTTTCT CTGCCGGATT CACCCAGATC 
CTGGCCCCGC AGGCTGTTGG GTCTGGCATC CCCGAGATGA AAACCATCCT TCGGGGAGTG 
GTGCTGAAAG AGTACCTCAC CCTCAAGACC TTTGTAGCTA AGGTCATCGG GCTGACCTGT 
GCCCTGGGCA GTGGGATGCC CCTTGGCAAG GAGGGACCCT TTGTGCACAT TGCCAGCATG 
TGTGCTGCCC TTCTCAGCAA GTTCCTCTCC CTCTTTGGGG GTATCTATGA GAACGAGTCC 
CGGAACACAG AGATGCTAGC TGCCGCATGC GCAGTAGGAG TGGGCTGCTG CTTTGCCGCA 
CCAATCGGAG GGGTCCTATT CAGCATTGAG GTCACCTCCA CCTTCTTCGC TGTTAGGAAC 
TACTGGCGGG GCTTCTTTGC GGCCACCTTC AGTGCCTTCA TCTTTCGGGT CTTGGCAGTG 
TGGAACCGTG ATGAAGAAAC CATCACAGCT CTCTTCAAAA CTCGGTTCCG ACTCGACTTC 
CCCTTTGACC TGCAGGAGCT GCCAGCTTTT GCTGTCATTG GTATTGCTAG TGGCTTCGGG 
GGAGCCCTCT TTGTCTACCT GAACCGGAAG ATTGTCCAGG TGATGCGGAA GCAAAAAACC 
ATCAACCGCT TCCTCATGAA GAAACGGCTG CTCTTCCCCG CACTGGTGAC TCTGCTCATC 
TCCACTCTGA CCTTCCCCCC TGGCTTTGGA CAGTTCATGG CCGGACAGCT CTCACAGAAA 
GAGACCCTGG TCACACTGTT TGACAACCGG ACATGGGTCC GCCAGGGCCT GGTAGAGGAT 
CTAGGAGCAC CTAGCACTTC ACAGGCCTGG AGCCCACCGC GCGCCAACGT CTTCCTGACT 
CTCGTCATCT TCATCCTCAT GAAGTTCTGG ATGTCTGCAC TGGCCACCAC CATCCCAGTG 
CCCTGTGGTG CCTTCATGCC TGTCTTTGTC ATTGGAGCAG CATTTGGCCG GCTGGTGGGC 
GAGAGCATGG CTGCCTGGTT CCCAGATGGG ATTCACACGG ATAGCAGTAC CTACCGGATT 
GTACCTGGAG GCTATGCCGT GGTCGGGGCA GCTGCACTTG CAGGAGCAGT GACACACACA 
GTGTCCACAG CAGTGATTGT CTTCGAGCTC ACCGGCCAGA TTGCCCACAT TCTGCCCGTC 
ATGATTGCTG TCATCCTGGC GAATGCCGTT GCCCAGAGCC TGCAGCCCTC CCTCTATGAC 
AGTATCATCC GCATCAAGAA GCTGCCCTAC CTGCCTGAGC TGGGCTGGGG CCGCCACCAG 
CAGTACCGGG TACGAGTAGA GGACATCATG GTACGGGATG TACCCCATGT AGCCCTCAGC 
TGCACTTTTC GGGACCTGCG ACTGGCACTG CACCGAACCA AGGGCCGTAT GTTGGCCCTA 
GTGGAGTCAC CTGAGTCCAT GATTCTGCTG GGCTCCATCG AACGCTCACA GGTGGTAGCG 
CTACTGGGAG CACAGCTGAG TCCAGCGCGC CGGCGGCAGC ACATGCAAAA GCTAAGGAAA 
GCCCAGATGT CCCCACCGTC AGATCAGGAG AGCCCCCCCA GCTCTGAGAC GTCCATCCGC 
TTCCAGGTGA ACACAGAGGA CTCAGGCTTC CCTGGAGCCC ATGGGCAGAC TCACAAGCCC 
CTGAAGCCTG CTCTAAAGAG AGGGCCCAGC AACGCCACAA GCCTCCAGGA AGGTACCACG 
GGCAACATGG AATCAGCAGG CATTGCCCTC AGAAGCCTCT TCTGTGGCAG TCCACCTCTG 
GAGTCGACGA CGTCAGAATT GGAAAAGTCG GAATCCTGTG ACAAACGCAA GCTGAAGCGG 
GTCCGAATCT CCCTGGCGAG TGACTCAGAC CTGGAAGGCA AGATGAGTCC CGAGGAGATC 
TTAGAGTGGG AAGAGCAGCA GCTAGATGAG CCAGTCAACT TCAGTGACTG CAAAATCGAC 
CCCGCCCCCT TCCAGCTAGT GGAGCGGACT TCTTTGCACA AGACCCACAC CATCTTCTCG 
CTGCTGGGAG TGGACCATGC TTATGTCACC AGCATTGGCA GACTCATTGG GATTGTCACC 
CTAAAGGAGC TCCGGAAGGC CATTGAAGGC TCTGTCACAG CACAGGGTGT AAAAGTCAGG 
CCACCCCTTG CCAGTTTCCG GGACAGTGCC ACCAGCAGCA GTGACACAGA GACCACCGAG 
GTGCATGCAC TCTGGGGGCC GAGATCCCGC CACGGCCTCC CACGAGAGGG TACCCCCTCT 
GATAGTGATG ACAAGTGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activated ClC-2 Inhibits p-Akt to Repress Myelination in GDM Newborn Rats.
International journal of biological sciences13(2)179-188(2017)
He F,Peng Y,Yang Z,Ge Z,Tian Y,Ma T,Li H

ClC-2 contributes to tonic inhibition mediated by ?5 subunit-containing GABA(A) receptor in experimental temporal lobe epilepsy.
Neuroscience186120-7(2011 Jul)
Ge YX,Liu Y,Tang HY,Liu XG,Wang X

Mouse model resources for vision research.
Journal of ophthalmology2011391384(2011)
Won J,Shi LY,Hicks W,Wang J,Hurd R,Naggert JK,Chang B,Nishina PM

Regulation of fast-spiking basket cell synapses by the chloride channel ClC-2.
Nature neuroscience13(9)1047-9(2010 Sep)
F?ldy C,Lee SH,Morgan RJ,Soltesz I

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Clcn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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