Hpca cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hpca
Entrez Gene ID	29177
Full Name	hippocalcin
General protein information	Preferred Names hippocalcin Names neuron-specific calcium-binding protein hippocalcin P23K calcium-binding protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	calcium-binding protein of the recoverin family [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017122.1	NP_058818.1	neuron-specific calcium-binding protein hippocalcin
XM_006238920.2	XP_006238982.1	neuron-specific calcium-binding protein hippocalcin isoform X1
XM_006238921.3	XP_006238983.1	neuron-specific calcium-binding protein hippocalcin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HPCA	NP_002134.2
Rattus norvegicus (Norway rat)	Hpca	NP_058818.1
Xenopus tropicalis (tropical clawed frog)	hpca	NP_001037936.1
Gallus gallus (chicken)	HPCA	XP_001233529.1
Canis lupus familiaris (dog)	HPCA	XP_854592.2
Bos taurus (cattle)	HPCA	NP_001020499.1
Mus musculus (house mouse)	Hpca	NP_001273012.1
Pan troglodytes (chimpanzee)	HPCA	XP_001155509.2
	HPCA	XP_001098851.2
Danio rerio (zebrafish)	hpca	NP_957017.1

Gene Ontology
Bibliography

Related articles in PubMed

Diabetes aggravates decreases in hippocalcin and parvalbumin expression in focal cerebral ischemia.
Park DJ, Koh PO
Neuroscience letters662189-194(2018 Jan)

Biophysical and functional characterization of hippocalcin mutants responsible for human dystonia.
Helassa N, Antonyuk SV, Lian LY, Haynes LP, Burgoyne RD
Human molecular genetics26(13)2426-2435(2017 07)

Expression profiling shows differential molecular pathways and provides potential new diagnostic biomarkers for colorectal serrated adenocarcinoma.
Conesa-Zamora P, Garc?a-Solano J, Garc?a-Garc?a F, Turpin Mdel C, Trujillo-Santos J, Torres-Moreno D, Oviedo-Ram?rez I, Carbonell-Mu?oz R, Mu?oz-Delgado E, Rodriguez-Braun E, Conesa A, P?rez-Guillermo M
International journal of cancer132(2)297-307(2013 Jan)

Hippocalcin mediates calcium-dependent translocation of brain-type creatine kinase (BB-CK) in hippocampal neurons.
Kobayashi M, Hamanoue M, Masaki T, Furuta Y, Takamatsu K
Biochemical and biophysical research communications429(3-4)142-7(2012 Dec)

Melatonin regulates the calcium-buffering proteins, parvalbumin and hippocalcin, in ischemic brain injury.
Koh PO
Journal of pineal research53(4)358-65(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that Hpca associates with BB-CK and that together they translocate to membrane compartments in a Ca(2+)-dependent manner.
Title: Hippocalcin mediates calcium-dependent translocation of brain-type creatine kinase (BB-CK) in hippocampal neurons.

Suggest that the maintenance of parvalbumin and hippocalcin levels by melatonin in ischemic injury contributes to the neuroprotective effect of melatonin against neuronal cell damage.
Title: Melatonin regulates the calcium-buffering proteins, parvalbumin and hippocalcin, in ischemic brain injury.

hippocalcin may differentially decode patterns of glutamate receptor activation into site- and time-specific translocation to its targets. Hippocalcin can also produce local signaling at synapses providing a molecular mechanism for homosynaptic plasticity
Title: Decoding glutamate receptor activation by the Ca2+ sensor protein hippocalcin in rat hippocampal neurons.

Taken together, these results suggest for the first time that hippocalcin can induce PLD2 expression and LPA potentiates hippocalcin-induced PLD2 expression, which is mediated by ERK activation.
Title: Hippocalcin increases phospholipase D2 expression through extracellular signal-regulated kinase activation and lysophosphatidic acid potentiates the hippocalcin-induced phospholipase D2 expression.

These data indicate that the AP2-hippocalcin complex acts as a Ca(2+) sensor that couples NMDAR-dependent activation to regulated endocytosis of AMPARs during LTD.
Title: Hippocalcin functions as a calcium sensor in hippocampal LTD.

The following Hpca gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hpca cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02931	NM_017122.1 Latest version!	Rattus norvegicus hippocalcin (Hpca), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa02931	XM_006238921.3 Latest version!	Rattus norvegicus hippocalcin (Hpca), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa02931	XM_006238920.2 Latest version!	Rattus norvegicus hippocalcin (Hpca), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hpca ( NM_017122.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058818.1 Rattus norvegicus hippocalcin (Hpca), mRNA.

Price & Availability↑

CloneID	ORa02931
Clone ID Related Accession (Same CDS sequence)	NM_017122.1 , XM_006238920.2 , XM_006238921.3
Accession Version	NM_017122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	neuron-specific calcium-binding protein hippocalcin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D12573.1. On Dec 17, 2003 this sequence version replaced XM_346751.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D12573.1, CF976762.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAAGC AGAATAGCAA GCTGCGGCCA GAGATGCTGC AGGACCTGCG AGAGAACACC 
GAGTTCTCTG AGCTGGAGCT TCAGGAGTGG TACAAGGGCT TCCTGAAGGA CTGCCCGACT 
GGCATCCTCA ACGTGGATGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAT 
GCCTCCAAGT TCGCCGAGCA TGTCTTCCGC ACTTTTGACA CCAACAGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT CGCTCTGAGC GTGACCTCGC GTGGCCGCCT GGAGCAGAAG 
CTCATGTGGG CCTTCAGCAT GTACGACCTG GACGGCAATG GCTACATCAG CCGGGAGGAG 
ATGCTAGAAA TTGTGCAGGC CATTTACAAG ATGGTTTCGT CCGTGATGAA GATGCCTGAG 
GATGAGTCTA CCCCGGAAAA GAGGACTGAG AAAATCTTCC GCCAAATGGA CACAAACAAT 
GACGGCAAGC TGTCACTGGA GGAGTTCATC CGCGGGGCCA AAAGCGACCC ATCGATCGTG 
CGCCTGCTGC AATGCGATCC CAGCAGCGCT TCCCAGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058818.1
CDS	175..756
Translation	MGKQNSKLRPEMLQDLRENTEFSELELQEWYKGFLKDCPTGILNVDEFKKIYANFFPYGDASKFAEHVFRTFDTNSDGTIDFREFIIALSVTSRGRLEQKLMWAFSMYDLDGNGYISREEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNNDGKLSLEEFIRGAKSDPSIVRLLQCDPSSASQF

Target ORF information:

RefSeq Version	NM_017122.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hippocalcin (Hpca), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017122.1

ORF Insert Sequence:

ATGGGCAAGC AGAATAGCAA GCTGCGGCCA GAGATGCTGC AGGACCTGCG AGAGAACACC 
GAGTTCTCTG AGCTGGAGCT TCAGGAGTGG TACAAGGGCT TCCTGAAGGA CTGCCCGACT 
GGCATCCTCA ACGTGGATGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAT 
GCCTCCAAGT TCGCCGAGCA TGTCTTCCGC ACTTTTGACA CCAACAGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT CGCTCTGAGC GTGACCTCGC GTGGCCGCCT GGAGCAGAAG 
CTCATGTGGG CCTTCAGCAT GTACGACCTG GACGGCAATG GCTACATCAG CCGGGAGGAG 
ATGCTAGAAA TTGTGCAGGC CATTTACAAG ATGGTTTCGT CCGTGATGAA GATGCCTGAG 
GATGAGTCTA CCCCGGAAAA GAGGACTGAG AAAATCTTCC GCCAAATGGA CACAAACAAT 
GACGGCAAGC TGTCACTGGA GGAGTTCATC CGCGGGGCCA AAAGCGACCC ATCGATCGTG 
CGCCTGCTGC AATGCGATCC CAGCAGCGCT TCCCAGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hpca ( XM_006238920.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006238982.1 Rattus norvegicus hippocalcin (Hpca), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02931
Clone ID Related Accession (Same CDS sequence)	NM_017122.1 , XM_006238920.2 , XM_006238921.3
Accession Version	XM_006238920.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuron-specific calcium-binding protein hippocalcin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238920.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAGC AGAATAGCAA GCTGCGGCCA GAGATGCTGC AGGACCTGCG AGAGAACACC 
GAGTTCTCTG AGCTGGAGCT TCAGGAGTGG TACAAGGGCT TCCTGAAGGA CTGCCCGACT 
GGCATCCTCA ACGTGGATGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAT 
GCCTCCAAGT TCGCCGAGCA TGTCTTCCGC ACTTTTGACA CCAACAGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT CGCTCTGAGC GTGACCTCGC GTGGCCGCCT GGAGCAGAAG 
CTCATGTGGG CCTTCAGCAT GTACGACCTG GACGGCAATG GCTACATCAG CCGGGAGGAG 
ATGCTAGAAA TTGTGCAGGC CATTTACAAG ATGGTTTCGT CCGTGATGAA GATGCCTGAG 
GATGAGTCTA CCCCGGAAAA GAGGACTGAG AAAATCTTCC GCCAAATGGA CACAAACAAT 
GACGGCAAGC TGTCACTGGA GGAGTTCATC CGCGGGGCCA AAAGCGACCC ATCGATCGTG 
CGCCTGCTGC AATGCGATCC CAGCAGCGCT TCCCAGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006238982.1
CDS	124..705
Translation	MGKQNSKLRPEMLQDLRENTEFSELELQEWYKGFLKDCPTGILNVDEFKKIYANFFPYGDASKFAEHVFRTFDTNSDGTIDFREFIIALSVTSRGRLEQKLMWAFSMYDLDGNGYISREEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNNDGKLSLEEFIRGAKSDPSIVRLLQCDPSSASQF

Target ORF information:

RefSeq Version	XM_006238920.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hippocalcin (Hpca), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238920.2

ORF Insert Sequence:

ATGGGCAAGC AGAATAGCAA GCTGCGGCCA GAGATGCTGC AGGACCTGCG AGAGAACACC 
GAGTTCTCTG AGCTGGAGCT TCAGGAGTGG TACAAGGGCT TCCTGAAGGA CTGCCCGACT 
GGCATCCTCA ACGTGGATGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAT 
GCCTCCAAGT TCGCCGAGCA TGTCTTCCGC ACTTTTGACA CCAACAGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT CGCTCTGAGC GTGACCTCGC GTGGCCGCCT GGAGCAGAAG 
CTCATGTGGG CCTTCAGCAT GTACGACCTG GACGGCAATG GCTACATCAG CCGGGAGGAG 
ATGCTAGAAA TTGTGCAGGC CATTTACAAG ATGGTTTCGT CCGTGATGAA GATGCCTGAG 
GATGAGTCTA CCCCGGAAAA GAGGACTGAG AAAATCTTCC GCCAAATGGA CACAAACAAT 
GACGGCAAGC TGTCACTGGA GGAGTTCATC CGCGGGGCCA AAAGCGACCC ATCGATCGTG 
CGCCTGCTGC AATGCGATCC CAGCAGCGCT TCCCAGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hpca ( XM_006238921.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006238983.1 Rattus norvegicus hippocalcin (Hpca), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa02931
Clone ID Related Accession (Same CDS sequence)	NM_017122.1 , XM_006238920.2 , XM_006238921.3
Accession Version	XM_006238921.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuron-specific calcium-binding protein hippocalcin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238921.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAGC AGAATAGCAA GCTGCGGCCA GAGATGCTGC AGGACCTGCG AGAGAACACC 
GAGTTCTCTG AGCTGGAGCT TCAGGAGTGG TACAAGGGCT TCCTGAAGGA CTGCCCGACT 
GGCATCCTCA ACGTGGATGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAT 
GCCTCCAAGT TCGCCGAGCA TGTCTTCCGC ACTTTTGACA CCAACAGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT CGCTCTGAGC GTGACCTCGC GTGGCCGCCT GGAGCAGAAG 
CTCATGTGGG CCTTCAGCAT GTACGACCTG GACGGCAATG GCTACATCAG CCGGGAGGAG 
ATGCTAGAAA TTGTGCAGGC CATTTACAAG ATGGTTTCGT CCGTGATGAA GATGCCTGAG 
GATGAGTCTA CCCCGGAAAA GAGGACTGAG AAAATCTTCC GCCAAATGGA CACAAACAAT 
GACGGCAAGC TGTCACTGGA GGAGTTCATC CGCGGGGCCA AAAGCGACCC ATCGATCGTG 
CGCCTGCTGC AATGCGATCC CAGCAGCGCT TCCCAGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006238983.1
CDS	98..679
Translation	MGKQNSKLRPEMLQDLRENTEFSELELQEWYKGFLKDCPTGILNVDEFKKIYANFFPYGDASKFAEHVFRTFDTNSDGTIDFREFIIALSVTSRGRLEQKLMWAFSMYDLDGNGYISREEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNNDGKLSLEEFIRGAKSDPSIVRLLQCDPSSASQF

Target ORF information:

RefSeq Version	XM_006238921.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hippocalcin (Hpca), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238921.3

ORF Insert Sequence:

ATGGGCAAGC AGAATAGCAA GCTGCGGCCA GAGATGCTGC AGGACCTGCG AGAGAACACC 
GAGTTCTCTG AGCTGGAGCT TCAGGAGTGG TACAAGGGCT TCCTGAAGGA CTGCCCGACT 
GGCATCCTCA ACGTGGATGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAT 
GCCTCCAAGT TCGCCGAGCA TGTCTTCCGC ACTTTTGACA CCAACAGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT CGCTCTGAGC GTGACCTCGC GTGGCCGCCT GGAGCAGAAG 
CTCATGTGGG CCTTCAGCAT GTACGACCTG GACGGCAATG GCTACATCAG CCGGGAGGAG 
ATGCTAGAAA TTGTGCAGGC CATTTACAAG ATGGTTTCGT CCGTGATGAA GATGCCTGAG 
GATGAGTCTA CCCCGGAAAA GAGGACTGAG AAAATCTTCC GCCAAATGGA CACAAACAAT 
GACGGCAAGC TGTCACTGGA GGAGTTCATC CGCGGGGCCA AAAGCGACCC ATCGATCGTG 
CGCCTGCTGC AATGCGATCC CAGCAGCGCT TCCCAGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diabetes aggravates decreases in hippocalcin and parvalbumin expression in focal cerebral ischemia.
Neuroscience letters662189-194(2018 Jan)
Park DJ,Koh PO

Biophysical and functional characterization of hippocalcin mutants responsible for human dystonia.
Human molecular genetics26(13)2426-2435(2017 07)
Helassa N,Antonyuk SV,Lian LY,Haynes LP,Burgoyne RD

Expression profiling shows differential molecular pathways and provides potential new diagnostic biomarkers for colorectal serrated adenocarcinoma.
International journal of cancer132(2)297-307(2013 Jan)
Conesa-Zamora P,Garc?a-Solano J,Garc?a-Garc?a F,Turpin Mdel C,Trujillo-Santos J,Torres-Moreno D,Oviedo-Ram?rez I,Carbonell-Mu?oz R,Mu?oz-Delgado E,Rodriguez-Braun E,Conesa A,P?rez-Guillermo M

Hippocalcin mediates calcium-dependent translocation of brain-type creatine kinase (BB-CK) in hippocampal neurons.
Biochemical and biophysical research communications429(3-4)142-7(2012 Dec)
Kobayashi M,Hamanoue M,Masaki T,Furuta Y,Takamatsu K

Melatonin regulates the calcium-buffering proteins, parvalbumin and hippocalcin, in ischemic brain injury.
Journal of pineal research53(4)358-65(2012 Nov)
Koh PO

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Hpca cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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