Capn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Capn1
Entrez Gene ID	29153
Full Name	calpain 1
General protein information	Preferred Names calpain 1 Names calpain-1 catalytic subunit CANP 1 calcium-activated neutral proteinase 1 calpain 1, (mu/I) large subunit calpain mu-type calpain-1 large subunit micromolar-calpain muCANP
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	calcium-dependent protease that may be involved in experimental allergic encephalomyelitis (EAE) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008760088.2	XP_008758310.1	calpain-1 catalytic subunit isoform X1
XM_008760089.2	XP_008758311.1	calpain-1 catalytic subunit isoform X1
XM_006230706.3	XP_006230768.1	calpain-1 catalytic subunit isoform X2
NM_019152.2	NP_062025.1	calpain-1 catalytic subunit

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAPN1	XP_001169100.1
Mus musculus (house mouse)	Capn1	NP_001103974.1
Bos taurus (cattle)	CAPN1	NP_776684.1
Danio rerio (zebrafish)	si:dkeyp-50d11.2	XP_689397.5
Xenopus tropicalis (tropical clawed frog)	capn1	NP_001013632.1
Gallus gallus (chicken)	CAPN1	NP_001038137.1
Danio rerio (zebrafish)	zgc:55262	NP_998624.1
Homo sapiens (human)	CAPN1	NP_001185797.1
	CAPN1	XP_001114172.1
Canis lupus familiaris (dog)	CAPN1	XP_540866.2
Rattus norvegicus (Norway rat)	Capn1	NP_062025.1

Gene Ontology
Bibliography

Related articles in PubMed

Neuroprotective Effects of Oxytocin Hormone after an Experimental Stroke Model and the Possible Role of Calpain-1.
Etehadi Moghadam S, Azami Tameh A, Vahidinia Z, Atlasi MA, Hassani Bafrani H, Naderian H
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association(2017 Dec)

CaMKII inhibition mitigates ischemia/reperfusion-elicited calpain activation and the damage to membrane skeleton proteins in isolated rat hearts.
Kong LH, Gu XM, Wu F, Jin ZX, Zhou JJ
Biochemical and biophysical research communications491(3)687-692(2017 09)

Calpain-GRIP Signaling in Nucleus Accumbens Core Mediates the Reconsolidation of Drug Reward Memory.
Liang J, Li JL, Han Y, Luo YX, Xue YX, Zhang Y, Zhang Y, Zhang LB, Chen ML, Lu L, Shi J
The Journal of neuroscience : the official journal of the Society for Neuroscience37(37)8938-8951(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In conclusion, our data provide evidence that damage to membrane skeleton proteins via calpain is a destructive downstream event of CaMKII activation in the setting of myocardial IR injury.
Title: CaMKII inhibition mitigates ischemia/reperfusion-elicited calpain activation and the damage to membrane skeleton proteins in isolated rat hearts.

Our findings indicate that calpain-GRIP signaling is essential for the restabilization process that is associated with drug cue memory and the inhibition of calpain activity may be a novel strategy for the prevention of drug relapse.
Title: Calpain-GRIP Signaling in Nucleus Accumbens Core Mediates the Reconsolidation of Drug Reward Memory.

Our results are supportive of the hypothesis that suppression of calpain expression leading to slowing of cell migration is a potential mechanism through which NSAIDs cause GI toxicity.
Title: Suppression of calpain expression by NSAIDs is associated with inhibition of cell migration in rat duodenum.

hese results suggest that the protein adjustments observed in lipid raft/caveolin-1 microdomains could be visualized as a process required to protect the cells against NO overproduction and aberrant calpain activation. Alterations in eNOS, calpain-1 and HSP90 levels have been observed in aorta of Zucker Diabetic Rats (ZDR).
Title: Calpain-1 resident in lipid raft/caveolin-1 membrane microdomains plays a protective role in endothelial cells.

Results indicate that calpain-1 and calpain-2 are differentially activated in hippocampus following seizure activity, with calpain-1 activation being limited to a small population of interneurons, and possibly triggering neuroprotective events, while calpain-2 is widely activated in pyramidal neurons of CA1 and CA3, and triggers neurodegenerative cascades.
Title: Differential Activation of Calpain-1 and Calpain-2 following Kainate-Induced Seizure Activity in Rats and Mice.

The following Capn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Capn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12831	NM_019152.2 Latest version!	Rattus norvegicus calpain 1 (Capn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa23706	XM_006230706.3 Latest version!	Rattus norvegicus calpain 1 (Capn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa43222	XM_008760088.2 Latest version!	Rattus norvegicus calpain 1 (Capn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa43222	XM_008760089.2 Latest version!	Rattus norvegicus calpain 1 (Capn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Capn1 ( NM_019152.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062025.1 Rattus norvegicus calpain 1 (Capn1), mRNA.

Price & Availability↑

CloneID	ORa12831
Clone ID Related Accession (Same CDS sequence)	NM_019152.2
Accession Version	NM_019152.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Rattus norvegicus(Norway rat)
Product	calpain-1 catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061880.1. On Feb 9, 2004 this sequence version replaced NM_019152.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061880.1, U53858.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGAGG AGTTAATCAC ACCTGTGTAC TGCACCGGGG TGTCAGCCCA AGTGCAGAAG 
CAACGGGACA AGGAGTTGGG CTTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAAA ATCTTCGTGC AAGATGCCTG CAGAACGGGG TCCTCTTCCA AGACGATGCC 
TTCCCTCCAG TTTCTCATAG CTTGGGCTTC AAGGAACTGG GTCCCAATTC CTCTAAAACC 
TATGGCATCA AGTGGAAGCG TCCTACGGAA CTGCTGTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GAACGGACAT CTGCCAGGGA GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATTGCCTCGC TCACTCTCAA CGAGACTATC CTCCACCGAG TGGTTCCCTA CGGCCAGAGC 
TTCCAGGAGG GCTATGCTGG CATCTTTCAT TTCCAGCTGT GGCAGTTTGG GGAGTGGGTA 
GATGTGGTTG TGGATGATTT GCTACCCACC AAGGATGGAA AGCTGGTGTT CGTGCACTCT 
GCCCAGGGCA ACGAGTTCTG GAGCGCTCTC CTGGAGAAAG CCTATGCAAA AGTGAATGGC 
AGCTATGAGG CCCTTTCGGG AGGCTGCACC TCAGAGGCCT TCGAGGACTT TACCGGTGGG 
GTCACTGAGT GGTACGATCT GCAGAAGGCA CCCAGCGACC TCTACCAGAT CATTCTGAAG 
GCCCTGGAAC GAGGCTCCTT GCTGGGCTGC TCCATTAATA TCTCCGATAT CCGTGATTTG 
GAGGCTATTA CTTTTAAGAA CCTGGTGAGG GGCCATGCAT ACTCTGTGAC GGATGCCAAG 
CAGGTCACTT ACCAGGGCCA GCGGGTGAAC CTAATCCGGA TGCGGAACCC CTGGGGTGAA 
GTGGAGTGGA AAGGACCCTG GAGTGACAAC TCCTATGAGT GGAACAAAGT GGACCCCTAT 
GAACGTGAGC AGCTGAGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC CTTCCGGGAC 
TTCATACGTG AGTTCACCAA ACTGGAAATC TGCAACCTTA CACCTGACGC CCTTAAGAGC 
AGAACCCTCC GGAACTGGAA TACCACATTT TACGAGGGCA CCTGGCGCCG GGGAAGCACT 
GCTGGAGGCT GCAGGAACTA CCCAGCTACC TTCTGGGTAA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGG TGGATGACGC AGACGACTAT GACAGTCGAG AGTCCGGCTG CAGCTTCTTG 
TTGGCCCTCA TGCAGAAGCA TCGCCGCAGG GAGCGTCGCT TTGGCCGGGA CATGGAGACC 
ATTGGTTTTG CAGTGTACCA GGTCCCTCGG GAGTTGGCGG GTCAGCCTGT GCACTTGAAG 
CGTGACTTCT TCCTGGCCAA TGCTTCTCGG GCACAATCAG AGCACTTTAT CAACCTTCGA 
GAGGTCAGTA ACCGCATCCG CCTGCCGCCG GGGGAGTATA TAGTGGTGCC CTCCACCTTC 
GAGCCCAACA AAGAGGGTGA CTTTCTGCTG CGCTTCTTTT CAGAGAAGAA GGCTGGGACC 
CAGGAACTAG ATGACCAGAT CCAGGCCAAC CTCCCCGATG AGAAAGTGCT CTCTGAAGAG 
GAGATCGATG ACAACTTCAA AACCCTGTTC AGCAAGTTGG CAGGGGATGA CATGGAGATC 
AGTGTCAAGG AGTTACAGAC CATCTTGAAC AGAATCATTA GCAAACACAA AGACCTGCGC 
ACTAACGGCT TCAGCCTGGA GTCGTGCCGC AGCATGGTGA ACCTCATGGA TCGAGACGGT 
AATGGGAAAC TGGGCCTGGT GGAGTTCAAC ATCCTGTGGA ACCGCATCCG AAATTACCTG 
ACCATCTTCC GAAAGTTTGA CCTGGACAAG TCTGGCAGCA TGAGTGCTTA TGAGATGAGG 
ATGGCCATCG AGGCTGCAGG CTTCAAGCTT AACAAGAAGC TGCATGAGCT CATCATCACC 
CGCTACTCAG AGCCCGACCT GGCCGTGGAC TTTGACAACT TTGTGTGCTG CCTTGTGCGC 
CTGGAGACCA TGTTCCGGTT TTTCAAAATT CTGGACACAG ACCTGGATGG TGTTGTGACC 
TTTGATCTAT TTAAGTGGTT ACAGCTGACT ATGTTTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062025.1
CDS	52..2193
Translation	MAEELITPVYCTGVSAQVQKQRDKELGLGRHENAIKYLGQDYENLRARCLQNGVLFQDDAFPPVSHSLGFKELGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQEGYAGIFHFQLWQFGEWVDVVVDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTDAKQVTYQGQRVNLIRMRNPWGEVEWKGPWSDNSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDSRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEISVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDKSGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKILDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	NM_019152.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 1 (Capn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019152.2

ORF Insert Sequence:

ATGGCAGAGG AGTTAATCAC ACCTGTGTAC TGCACCGGGG TGTCAGCCCA AGTGCAGAAG 
CAACGGGACA AGGAGTTGGG CTTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAAA ATCTTCGTGC AAGATGCCTG CAGAACGGGG TCCTCTTCCA AGACGATGCC 
TTCCCTCCAG TTTCTCATAG CTTGGGCTTC AAGGAACTGG GTCCCAATTC CTCTAAAACC 
TATGGCATCA AGTGGAAGCG TCCTACGGAA CTGCTGTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GAACGGACAT CTGCCAGGGA GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATTGCCTCGC TCACTCTCAA CGAGACTATC CTCCACCGAG TGGTTCCCTA CGGCCAGAGC 
TTCCAGGAGG GCTATGCTGG CATCTTTCAT TTCCAGCTGT GGCAGTTTGG GGAGTGGGTA 
GATGTGGTTG TGGATGATTT GCTACCCACC AAGGATGGAA AGCTGGTGTT CGTGCACTCT 
GCCCAGGGCA ACGAGTTCTG GAGCGCTCTC CTGGAGAAAG CCTATGCAAA AGTGAATGGC 
AGCTATGAGG CCCTTTCGGG AGGCTGCACC TCAGAGGCCT TCGAGGACTT TACCGGTGGG 
GTCACTGAGT GGTACGATCT GCAGAAGGCA CCCAGCGACC TCTACCAGAT CATTCTGAAG 
GCCCTGGAAC GAGGCTCCTT GCTGGGCTGC TCCATTAATA TCTCCGATAT CCGTGATTTG 
GAGGCTATTA CTTTTAAGAA CCTGGTGAGG GGCCATGCAT ACTCTGTGAC GGATGCCAAG 
CAGGTCACTT ACCAGGGCCA GCGGGTGAAC CTAATCCGGA TGCGGAACCC CTGGGGTGAA 
GTGGAGTGGA AAGGACCCTG GAGTGACAAC TCCTATGAGT GGAACAAAGT GGACCCCTAT 
GAACGTGAGC AGCTGAGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC CTTCCGGGAC 
TTCATACGTG AGTTCACCAA ACTGGAAATC TGCAACCTTA CACCTGACGC CCTTAAGAGC 
AGAACCCTCC GGAACTGGAA TACCACATTT TACGAGGGCA CCTGGCGCCG GGGAAGCACT 
GCTGGAGGCT GCAGGAACTA CCCAGCTACC TTCTGGGTAA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGG TGGATGACGC AGACGACTAT GACAGTCGAG AGTCCGGCTG CAGCTTCTTG 
TTGGCCCTCA TGCAGAAGCA TCGCCGCAGG GAGCGTCGCT TTGGCCGGGA CATGGAGACC 
ATTGGTTTTG CAGTGTACCA GGTCCCTCGG GAGTTGGCGG GTCAGCCTGT GCACTTGAAG 
CGTGACTTCT TCCTGGCCAA TGCTTCTCGG GCACAATCAG AGCACTTTAT CAACCTTCGA 
GAGGTCAGTA ACCGCATCCG CCTGCCGCCG GGGGAGTATA TAGTGGTGCC CTCCACCTTC 
GAGCCCAACA AAGAGGGTGA CTTTCTGCTG CGCTTCTTTT CAGAGAAGAA GGCTGGGACC 
CAGGAACTAG ATGACCAGAT CCAGGCCAAC CTCCCCGATG AGAAAGTGCT CTCTGAAGAG 
GAGATCGATG ACAACTTCAA AACCCTGTTC AGCAAGTTGG CAGGGGATGA CATGGAGATC 
AGTGTCAAGG AGTTACAGAC CATCTTGAAC AGAATCATTA GCAAACACAA AGACCTGCGC 
ACTAACGGCT TCAGCCTGGA GTCGTGCCGC AGCATGGTGA ACCTCATGGA TCGAGACGGT 
AATGGGAAAC TGGGCCTGGT GGAGTTCAAC ATCCTGTGGA ACCGCATCCG AAATTACCTG 
ACCATCTTCC GAAAGTTTGA CCTGGACAAG TCTGGCAGCA TGAGTGCTTA TGAGATGAGG 
ATGGCCATCG AGGCTGCAGG CTTCAAGCTT AACAAGAAGC TGCATGAGCT CATCATCACC 
CGCTACTCAG AGCCCGACCT GGCCGTGGAC TTTGACAACT TTGTGTGCTG CCTTGTGCGC 
CTGGAGACCA TGTTCCGGTT TTTCAAAATT CTGGACACAG ACCTGGATGG TGTTGTGACC 
TTTGATCTAT TTAAGTGGTT ACAGCTGACT ATGTTTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn1 ( XM_006230706.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230768.1 Rattus norvegicus calpain 1 (Capn1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa23706
Clone ID Related Accession (Same CDS sequence)	XM_006230706.3
Accession Version	XM_006230706.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calpain-1 catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG AGTTAATCAC ACCTGTGTAC TGCACCGGGG TGTCAGCCCA AGTGCAGAAG 
CAACGGGACA AGGAGTTGGG CTTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAAA ATCTTCGTGC AAGATGCCTG CAGAACGGGG TCCTCTTCCA AGACGATGCC 
TTCCCTCCAG TTTCTCATAG CTTGGGCTTC AAGGAACTGG GTCCCAATTC CTCTAAAACC 
TATGGCATCA AGTGGAAGCG TCCTACGGAA CTGCTGTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GAACGGACAT CTGCCAGGGA GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATTGCCTCGC TCACTCTCAA CGAGACTATC CTCCACCGAG TGGTTCCCTA CGGCCAGAGC 
TTCCAGGAGG GCTATGCTGG CATCTTTCAT TTCCAGCTGT GGCAGTTTGG GGAGTGGGTA 
GATGTGGTCG TGGATGATTT GCTACCCACC AAGGATGGAA AGCTGGTGTT CGTGCACTCT 
GCCCAGGGCA ACGAGTTCTG GAGCGCTCTC CTGGAGAAAG CCTATGCAAA AGTGAATGGC 
AGCTATGAGG CCCTTTCGGG AGGCTGCACC TCAGAGGCCT TCGAGGACTT TACCGGTGGG 
GTCACTGAGT GGTACGATCT GCAGAAGGCA CCCAGCGACC TCTACCAGAT CATTCTGAAG 
GCCCTGGAAC GAGGCTCCTT GCTGGGCTGC TCCATTAATA TCTCCGATAT CCGTGATTTG 
GAGGCTATTA CTTTTAAGAA CCTGGTGAGG GGCCATGCAT ACTCTGTGAC GGATGCCAAG 
CAGGTCACTT ACCAGGGCCA GCGGGTGAAC CTAATCCGGA TGCGGAACCC CTGGGGTGAA 
GTGGAGTGGA AAGGACCCTG GAGTGACAAC TCCTATGAGT GGAACAAAGT GGACCCCTAT 
GAACGTGAGC AGCTGAGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC CTTCCGGGAC 
TTCATACGTG AGTTCACCAA ACTGGAAATC TGCAACCTTA CACCTGACGC CCTTAAGAGC 
AGAACCCTCC GGAACTGGAA TACCACATTT TACGAGGGCA CCTGGCGCCG GGGAAGCACT 
GCTGGAGGCT GCAGGAACTA CCCAGCTACC TTCTGGGTAA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGG TGGATGACGC AGACGACTAT GACAGTCGAG AGTCCGGCTG CAGCTTCTTG 
TTGGCCCTCA TGCAGAAGCA TCGCCGCAGG GAGCGTCGCT TTGGCCGGGA CATGGAGACC 
ATTGGTTTTG CAGTGTACCA GGTCCCTCGG GAGTTGGCGG GTCAGCCTGT GCACTTGAAG 
CGTGACTTCT TCCTGGCCAA TGCTTCTCGG GCACAATCAG AGCACTTTAT CAACCTTCGA 
GAGGTCAGTA ACCGCATCCG CCTGCCGCCG GGGGAGTATA TAGTGGTGCC CTCCACCTTC 
GAGCCCAACA AAGAGGGTGA CTTTCTGCTG CGCTTCTTTT CAGAGAAGAA GGCTGGGACC 
CAGGAACTAG ATGACCAGAT CCAGGCCAAC CTCCCCGATG AGAAAGTGCT CTCTGAAGAG 
GAGATCGATG ACAACTTCAA AACCCTGTTC AGCAAGTTAG CAGGGGATGA CATGGAGATC 
AGTGTCAAGG AGTTACAGAC CATCTTGAAC AGAATCATTA GCAAACGGAG AACAGACAGC 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230768.1
CDS	102..1847
Translation	MAEELITPVYCTGVSAQVQKQRDKELGLGRHENAIKYLGQDYENLRARCLQNGVLFQDDAFPPVSHSLGFKELGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQEGYAGIFHFQLWQFGEWVDVVVDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTDAKQVTYQGQRVNLIRMRNPWGEVEWKGPWSDNSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDSRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEISVKELQTILNRIISKRRTDSP

Target ORF information:

RefSeq Version	XM_006230706.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 1 (Capn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230706.3

ORF Insert Sequence:

ATGGCAGAGG AGTTAATCAC ACCTGTGTAC TGCACCGGGG TGTCAGCCCA AGTGCAGAAG 
CAACGGGACA AGGAGTTGGG CTTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAAA ATCTTCGTGC AAGATGCCTG CAGAACGGGG TCCTCTTCCA AGACGATGCC 
TTCCCTCCAG TTTCTCATAG CTTGGGCTTC AAGGAACTGG GTCCCAATTC CTCTAAAACC 
TATGGCATCA AGTGGAAGCG TCCTACGGAA CTGCTGTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GAACGGACAT CTGCCAGGGA GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATTGCCTCGC TCACTCTCAA CGAGACTATC CTCCACCGAG TGGTTCCCTA CGGCCAGAGC 
TTCCAGGAGG GCTATGCTGG CATCTTTCAT TTCCAGCTGT GGCAGTTTGG GGAGTGGGTA 
GATGTGGTCG TGGATGATTT GCTACCCACC AAGGATGGAA AGCTGGTGTT CGTGCACTCT 
GCCCAGGGCA ACGAGTTCTG GAGCGCTCTC CTGGAGAAAG CCTATGCAAA AGTGAATGGC 
AGCTATGAGG CCCTTTCGGG AGGCTGCACC TCAGAGGCCT TCGAGGACTT TACCGGTGGG 
GTCACTGAGT GGTACGATCT GCAGAAGGCA CCCAGCGACC TCTACCAGAT CATTCTGAAG 
GCCCTGGAAC GAGGCTCCTT GCTGGGCTGC TCCATTAATA TCTCCGATAT CCGTGATTTG 
GAGGCTATTA CTTTTAAGAA CCTGGTGAGG GGCCATGCAT ACTCTGTGAC GGATGCCAAG 
CAGGTCACTT ACCAGGGCCA GCGGGTGAAC CTAATCCGGA TGCGGAACCC CTGGGGTGAA 
GTGGAGTGGA AAGGACCCTG GAGTGACAAC TCCTATGAGT GGAACAAAGT GGACCCCTAT 
GAACGTGAGC AGCTGAGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC CTTCCGGGAC 
TTCATACGTG AGTTCACCAA ACTGGAAATC TGCAACCTTA CACCTGACGC CCTTAAGAGC 
AGAACCCTCC GGAACTGGAA TACCACATTT TACGAGGGCA CCTGGCGCCG GGGAAGCACT 
GCTGGAGGCT GCAGGAACTA CCCAGCTACC TTCTGGGTAA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGG TGGATGACGC AGACGACTAT GACAGTCGAG AGTCCGGCTG CAGCTTCTTG 
TTGGCCCTCA TGCAGAAGCA TCGCCGCAGG GAGCGTCGCT TTGGCCGGGA CATGGAGACC 
ATTGGTTTTG CAGTGTACCA GGTCCCTCGG GAGTTGGCGG GTCAGCCTGT GCACTTGAAG 
CGTGACTTCT TCCTGGCCAA TGCTTCTCGG GCACAATCAG AGCACTTTAT CAACCTTCGA 
GAGGTCAGTA ACCGCATCCG CCTGCCGCCG GGGGAGTATA TAGTGGTGCC CTCCACCTTC 
GAGCCCAACA AAGAGGGTGA CTTTCTGCTG CGCTTCTTTT CAGAGAAGAA GGCTGGGACC 
CAGGAACTAG ATGACCAGAT CCAGGCCAAC CTCCCCGATG AGAAAGTGCT CTCTGAAGAG 
GAGATCGATG ACAACTTCAA AACCCTGTTC AGCAAGTTAG CAGGGGATGA CATGGAGATC 
AGTGTCAAGG AGTTACAGAC CATCTTGAAC AGAATCATTA GCAAACGGAG AACAGACAGC 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn1 ( XM_008760088.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758310.1 Rattus norvegicus calpain 1 (Capn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43222
Clone ID Related Accession (Same CDS sequence)	XM_008760088.2 , XM_008760089.2
Accession Version	XM_008760088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calpain-1 catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG AGTTAATCAC ACCTGTGTAC TGCACCGGGG TGTCAGCCCA AGTGCAGAAG 
CAACGGGACA AGGAGTTGGG CTTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAAA ATCTTCGTGC AAGATGCCTG CAGAACGGGG TCCTCTTCCA AGACGATGCC 
TTCCCTCCAG TTTCTCATAG CTTGGGCTTC AAGGAACTGG GTCCCAATTC CTCTAAAACC 
TATGGCATCA AGTGGAAGCG TCCTACGGAA CTGCTGTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GAACGGACAT CTGCCAGGGA GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATTGCCTCGC TCACTCTCAA CGAGACTATC CTCCACCGAG TGGTTCCCTA CGGCCAGAGC 
TTCCAGGAGG GCTATGCTGG CATCTTTCAT TTCCAGCTGT GGCAGTTTGG GGAGTGGGTA 
GATGTGGTCG TGGATGATTT GCTACCCACC AAGGATGGAA AGCTGGTGTT CGTGCACTCT 
GCCCAGGGCA ACGAGTTCTG GAGCGCTCTC CTGGAGAAAG CCTATGCAAA AGTGAATGGC 
AGCTATGAGG CCCTTTCGGG AGGCTGCACC TCAGAGGCCT TCGAGGACTT TACCGGTGGG 
GTCACTGAGT GGTACGATCT GCAGAAGGCA CCCAGCGACC TCTACCAGAT CATTCTGAAG 
GCCCTGGAAC GAGGCTCCTT GCTGGGCTGC TCCATTAATA TCTCCGATAT CCGTGATTTG 
GAGGCTATTA CTTTTAAGAA CCTGGTGAGG GGCCATGCAT ACTCTGTGAC GGATGCCAAG 
CAGGTCACTT ACCAGGGCCA GCGGGTGAAC CTAATCCGGA TGCGGAACCC CTGGGGTGAA 
GTGGAGTGGA AAGGACCCTG GAGTGACAAC TCCTATGAGT GGAACAAAGT GGACCCCTAT 
GAACGTGAGC AGCTGAGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC CTTCCGGGAC 
TTCATACGTG AGTTCACCAA ACTGGAAATC TGCAACCTTA CACCTGACGC CCTTAAGAGC 
AGAACCCTCC GGAACTGGAA TACCACATTT TACGAGGGCA CCTGGCGCCG GGGAAGCACT 
GCTGGAGGCT GCAGGAACTA CCCAGCTACC TTCTGGGTAA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGG TGGATGACGC AGACGACTAT GACAGTCGAG AGTCCGGCTG CAGCTTCTTG 
TTGGCCCTCA TGCAGAAGCA TCGCCGCAGG GAGCGTCGCT TTGGCCGGGA CATGGAGACC 
ATTGGTTTTG CAGTGTACCA GGTCCCTCGG GAGTTGGCGG GTCAGCCTGT GCACTTGAAG 
CGTGACTTCT TCCTGGCCAA TGCTTCTCGG GCACAATCAG AGCACTTTAT CAACCTTCGA 
GAGGTCAGTA ACCGCATCCG CCTGCCGCCG GGGGAGTATA TAGTGGTGCC CTCCACCTTC 
GAGCCCAACA AAGAGGGTGA CTTTCTGCTG CGCTTCTTTT CAGAGAAGAA GGCTGGGACC 
CAGGAACTAG ATGACCAGAT CCAGGCCAAC CTCCCCGATG AGAAAGTGCT CTCTGAAGAG 
GAGATCGATG ACAACTTCAA AACCCTGTTC AGCAAGTTAG CAGGGGATGA CATGGAGATC 
AGTGTCAAGG AGTTACAGAC CATCTTGAAC AGAATCATTA GCAAACACAA AGACCTGCGC 
ACTAACGGCT TCAGCCTGGA GTCGTGCCGC AGCATGGTGA ACCTCATGGA TCGAGACGGT 
AATGGGAAAC TGGGCCTGGT GGAGTTCAAC ATCCTGTGGA ACCGCATCCG AAATTACCTG 
ACCATCTTCC GAAAGTTTGA CCTGGACAAG TCTGGCAGCA TGAGTGCTTA TGAGATGAGG 
ATGGCCATCG AGGCTGCAGG CTTCAAGCTT AACAAGAAGC TGCATGAGCT CATCATCACC 
CACTACTCAG AGCCCGACCT GGCCGTGGAC TTTGACAACT TTGTGTGCTG CCTTGTGCGC 
CTGGAGACCA TGTTCCGGTT TTTCAAAATT CTGGACACAG ACCTGGATGG TGTTGTGACC 
TTTGATCTAT TTAAGTGGTT ACAGCTGACT ATGTTTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758310.1
CDS	131..2272
Translation	MAEELITPVYCTGVSAQVQKQRDKELGLGRHENAIKYLGQDYENLRARCLQNGVLFQDDAFPPVSHSLGFKELGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQEGYAGIFHFQLWQFGEWVDVVVDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTDAKQVTYQGQRVNLIRMRNPWGEVEWKGPWSDNSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDSRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEISVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDKSGSMSAYEMRMAIEAAGFKLNKKLHELIITHYSEPDLAVDFDNFVCCLVRLETMFRFFKILDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	XM_008760088.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 1 (Capn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760088.2

ORF Insert Sequence:

ATGGCAGAGG AGTTAATCAC ACCTGTGTAC TGCACCGGGG TGTCAGCCCA AGTGCAGAAG 
CAACGGGACA AGGAGTTGGG CTTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAAA ATCTTCGTGC AAGATGCCTG CAGAACGGGG TCCTCTTCCA AGACGATGCC 
TTCCCTCCAG TTTCTCATAG CTTGGGCTTC AAGGAACTGG GTCCCAATTC CTCTAAAACC 
TATGGCATCA AGTGGAAGCG TCCTACGGAA CTGCTGTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GAACGGACAT CTGCCAGGGA GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATTGCCTCGC TCACTCTCAA CGAGACTATC CTCCACCGAG TGGTTCCCTA CGGCCAGAGC 
TTCCAGGAGG GCTATGCTGG CATCTTTCAT TTCCAGCTGT GGCAGTTTGG GGAGTGGGTA 
GATGTGGTCG TGGATGATTT GCTACCCACC AAGGATGGAA AGCTGGTGTT CGTGCACTCT 
GCCCAGGGCA ACGAGTTCTG GAGCGCTCTC CTGGAGAAAG CCTATGCAAA AGTGAATGGC 
AGCTATGAGG CCCTTTCGGG AGGCTGCACC TCAGAGGCCT TCGAGGACTT TACCGGTGGG 
GTCACTGAGT GGTACGATCT GCAGAAGGCA CCCAGCGACC TCTACCAGAT CATTCTGAAG 
GCCCTGGAAC GAGGCTCCTT GCTGGGCTGC TCCATTAATA TCTCCGATAT CCGTGATTTG 
GAGGCTATTA CTTTTAAGAA CCTGGTGAGG GGCCATGCAT ACTCTGTGAC GGATGCCAAG 
CAGGTCACTT ACCAGGGCCA GCGGGTGAAC CTAATCCGGA TGCGGAACCC CTGGGGTGAA 
GTGGAGTGGA AAGGACCCTG GAGTGACAAC TCCTATGAGT GGAACAAAGT GGACCCCTAT 
GAACGTGAGC AGCTGAGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC CTTCCGGGAC 
TTCATACGTG AGTTCACCAA ACTGGAAATC TGCAACCTTA CACCTGACGC CCTTAAGAGC 
AGAACCCTCC GGAACTGGAA TACCACATTT TACGAGGGCA CCTGGCGCCG GGGAAGCACT 
GCTGGAGGCT GCAGGAACTA CCCAGCTACC TTCTGGGTAA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGG TGGATGACGC AGACGACTAT GACAGTCGAG AGTCCGGCTG CAGCTTCTTG 
TTGGCCCTCA TGCAGAAGCA TCGCCGCAGG GAGCGTCGCT TTGGCCGGGA CATGGAGACC 
ATTGGTTTTG CAGTGTACCA GGTCCCTCGG GAGTTGGCGG GTCAGCCTGT GCACTTGAAG 
CGTGACTTCT TCCTGGCCAA TGCTTCTCGG GCACAATCAG AGCACTTTAT CAACCTTCGA 
GAGGTCAGTA ACCGCATCCG CCTGCCGCCG GGGGAGTATA TAGTGGTGCC CTCCACCTTC 
GAGCCCAACA AAGAGGGTGA CTTTCTGCTG CGCTTCTTTT CAGAGAAGAA GGCTGGGACC 
CAGGAACTAG ATGACCAGAT CCAGGCCAAC CTCCCCGATG AGAAAGTGCT CTCTGAAGAG 
GAGATCGATG ACAACTTCAA AACCCTGTTC AGCAAGTTAG CAGGGGATGA CATGGAGATC 
AGTGTCAAGG AGTTACAGAC CATCTTGAAC AGAATCATTA GCAAACACAA AGACCTGCGC 
ACTAACGGCT TCAGCCTGGA GTCGTGCCGC AGCATGGTGA ACCTCATGGA TCGAGACGGT 
AATGGGAAAC TGGGCCTGGT GGAGTTCAAC ATCCTGTGGA ACCGCATCCG AAATTACCTG 
ACCATCTTCC GAAAGTTTGA CCTGGACAAG TCTGGCAGCA TGAGTGCTTA TGAGATGAGG 
ATGGCCATCG AGGCTGCAGG CTTCAAGCTT AACAAGAAGC TGCATGAGCT CATCATCACC 
CACTACTCAG AGCCCGACCT GGCCGTGGAC TTTGACAACT TTGTGTGCTG CCTTGTGCGC 
CTGGAGACCA TGTTCCGGTT TTTCAAAATT CTGGACACAG ACCTGGATGG TGTTGTGACC 
TTTGATCTAT TTAAGTGGTT ACAGCTGACT ATGTTTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn1 ( XM_008760089.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758311.1 Rattus norvegicus calpain 1 (Capn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43222
Clone ID Related Accession (Same CDS sequence)	XM_008760088.2 , XM_008760089.2
Accession Version	XM_008760089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calpain-1 catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG AGTTAATCAC ACCTGTGTAC TGCACCGGGG TGTCAGCCCA AGTGCAGAAG 
CAACGGGACA AGGAGTTGGG CTTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAAA ATCTTCGTGC AAGATGCCTG CAGAACGGGG TCCTCTTCCA AGACGATGCC 
TTCCCTCCAG TTTCTCATAG CTTGGGCTTC AAGGAACTGG GTCCCAATTC CTCTAAAACC 
TATGGCATCA AGTGGAAGCG TCCTACGGAA CTGCTGTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GAACGGACAT CTGCCAGGGA GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATTGCCTCGC TCACTCTCAA CGAGACTATC CTCCACCGAG TGGTTCCCTA CGGCCAGAGC 
TTCCAGGAGG GCTATGCTGG CATCTTTCAT TTCCAGCTGT GGCAGTTTGG GGAGTGGGTA 
GATGTGGTCG TGGATGATTT GCTACCCACC AAGGATGGAA AGCTGGTGTT CGTGCACTCT 
GCCCAGGGCA ACGAGTTCTG GAGCGCTCTC CTGGAGAAAG CCTATGCAAA AGTGAATGGC 
AGCTATGAGG CCCTTTCGGG AGGCTGCACC TCAGAGGCCT TCGAGGACTT TACCGGTGGG 
GTCACTGAGT GGTACGATCT GCAGAAGGCA CCCAGCGACC TCTACCAGAT CATTCTGAAG 
GCCCTGGAAC GAGGCTCCTT GCTGGGCTGC TCCATTAATA TCTCCGATAT CCGTGATTTG 
GAGGCTATTA CTTTTAAGAA CCTGGTGAGG GGCCATGCAT ACTCTGTGAC GGATGCCAAG 
CAGGTCACTT ACCAGGGCCA GCGGGTGAAC CTAATCCGGA TGCGGAACCC CTGGGGTGAA 
GTGGAGTGGA AAGGACCCTG GAGTGACAAC TCCTATGAGT GGAACAAAGT GGACCCCTAT 
GAACGTGAGC AGCTGAGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC CTTCCGGGAC 
TTCATACGTG AGTTCACCAA ACTGGAAATC TGCAACCTTA CACCTGACGC CCTTAAGAGC 
AGAACCCTCC GGAACTGGAA TACCACATTT TACGAGGGCA CCTGGCGCCG GGGAAGCACT 
GCTGGAGGCT GCAGGAACTA CCCAGCTACC TTCTGGGTAA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGG TGGATGACGC AGACGACTAT GACAGTCGAG AGTCCGGCTG CAGCTTCTTG 
TTGGCCCTCA TGCAGAAGCA TCGCCGCAGG GAGCGTCGCT TTGGCCGGGA CATGGAGACC 
ATTGGTTTTG CAGTGTACCA GGTCCCTCGG GAGTTGGCGG GTCAGCCTGT GCACTTGAAG 
CGTGACTTCT TCCTGGCCAA TGCTTCTCGG GCACAATCAG AGCACTTTAT CAACCTTCGA 
GAGGTCAGTA ACCGCATCCG CCTGCCGCCG GGGGAGTATA TAGTGGTGCC CTCCACCTTC 
GAGCCCAACA AAGAGGGTGA CTTTCTGCTG CGCTTCTTTT CAGAGAAGAA GGCTGGGACC 
CAGGAACTAG ATGACCAGAT CCAGGCCAAC CTCCCCGATG AGAAAGTGCT CTCTGAAGAG 
GAGATCGATG ACAACTTCAA AACCCTGTTC AGCAAGTTAG CAGGGGATGA CATGGAGATC 
AGTGTCAAGG AGTTACAGAC CATCTTGAAC AGAATCATTA GCAAACACAA AGACCTGCGC 
ACTAACGGCT TCAGCCTGGA GTCGTGCCGC AGCATGGTGA ACCTCATGGA TCGAGACGGT 
AATGGGAAAC TGGGCCTGGT GGAGTTCAAC ATCCTGTGGA ACCGCATCCG AAATTACCTG 
ACCATCTTCC GAAAGTTTGA CCTGGACAAG TCTGGCAGCA TGAGTGCTTA TGAGATGAGG 
ATGGCCATCG AGGCTGCAGG CTTCAAGCTT AACAAGAAGC TGCATGAGCT CATCATCACC 
CACTACTCAG AGCCCGACCT GGCCGTGGAC TTTGACAACT TTGTGTGCTG CCTTGTGCGC 
CTGGAGACCA TGTTCCGGTT TTTCAAAATT CTGGACACAG ACCTGGATGG TGTTGTGACC 
TTTGATCTAT TTAAGTGGTT ACAGCTGACT ATGTTTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758311.1
CDS	131..2272
Translation	MAEELITPVYCTGVSAQVQKQRDKELGLGRHENAIKYLGQDYENLRARCLQNGVLFQDDAFPPVSHSLGFKELGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQEGYAGIFHFQLWQFGEWVDVVVDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTDAKQVTYQGQRVNLIRMRNPWGEVEWKGPWSDNSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDSRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEISVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDKSGSMSAYEMRMAIEAAGFKLNKKLHELIITHYSEPDLAVDFDNFVCCLVRLETMFRFFKILDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	XM_008760089.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 1 (Capn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760089.2

ORF Insert Sequence:

ATGGCAGAGG AGTTAATCAC ACCTGTGTAC TGCACCGGGG TGTCAGCCCA AGTGCAGAAG 
CAACGGGACA AGGAGTTGGG CTTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAAA ATCTTCGTGC AAGATGCCTG CAGAACGGGG TCCTCTTCCA AGACGATGCC 
TTCCCTCCAG TTTCTCATAG CTTGGGCTTC AAGGAACTGG GTCCCAATTC CTCTAAAACC 
TATGGCATCA AGTGGAAGCG TCCTACGGAA CTGCTGTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GAACGGACAT CTGCCAGGGA GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATTGCCTCGC TCACTCTCAA CGAGACTATC CTCCACCGAG TGGTTCCCTA CGGCCAGAGC 
TTCCAGGAGG GCTATGCTGG CATCTTTCAT TTCCAGCTGT GGCAGTTTGG GGAGTGGGTA 
GATGTGGTCG TGGATGATTT GCTACCCACC AAGGATGGAA AGCTGGTGTT CGTGCACTCT 
GCCCAGGGCA ACGAGTTCTG GAGCGCTCTC CTGGAGAAAG CCTATGCAAA AGTGAATGGC 
AGCTATGAGG CCCTTTCGGG AGGCTGCACC TCAGAGGCCT TCGAGGACTT TACCGGTGGG 
GTCACTGAGT GGTACGATCT GCAGAAGGCA CCCAGCGACC TCTACCAGAT CATTCTGAAG 
GCCCTGGAAC GAGGCTCCTT GCTGGGCTGC TCCATTAATA TCTCCGATAT CCGTGATTTG 
GAGGCTATTA CTTTTAAGAA CCTGGTGAGG GGCCATGCAT ACTCTGTGAC GGATGCCAAG 
CAGGTCACTT ACCAGGGCCA GCGGGTGAAC CTAATCCGGA TGCGGAACCC CTGGGGTGAA 
GTGGAGTGGA AAGGACCCTG GAGTGACAAC TCCTATGAGT GGAACAAAGT GGACCCCTAT 
GAACGTGAGC AGCTGAGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC CTTCCGGGAC 
TTCATACGTG AGTTCACCAA ACTGGAAATC TGCAACCTTA CACCTGACGC CCTTAAGAGC 
AGAACCCTCC GGAACTGGAA TACCACATTT TACGAGGGCA CCTGGCGCCG GGGAAGCACT 
GCTGGAGGCT GCAGGAACTA CCCAGCTACC TTCTGGGTAA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGG TGGATGACGC AGACGACTAT GACAGTCGAG AGTCCGGCTG CAGCTTCTTG 
TTGGCCCTCA TGCAGAAGCA TCGCCGCAGG GAGCGTCGCT TTGGCCGGGA CATGGAGACC 
ATTGGTTTTG CAGTGTACCA GGTCCCTCGG GAGTTGGCGG GTCAGCCTGT GCACTTGAAG 
CGTGACTTCT TCCTGGCCAA TGCTTCTCGG GCACAATCAG AGCACTTTAT CAACCTTCGA 
GAGGTCAGTA ACCGCATCCG CCTGCCGCCG GGGGAGTATA TAGTGGTGCC CTCCACCTTC 
GAGCCCAACA AAGAGGGTGA CTTTCTGCTG CGCTTCTTTT CAGAGAAGAA GGCTGGGACC 
CAGGAACTAG ATGACCAGAT CCAGGCCAAC CTCCCCGATG AGAAAGTGCT CTCTGAAGAG 
GAGATCGATG ACAACTTCAA AACCCTGTTC AGCAAGTTAG CAGGGGATGA CATGGAGATC 
AGTGTCAAGG AGTTACAGAC CATCTTGAAC AGAATCATTA GCAAACACAA AGACCTGCGC 
ACTAACGGCT TCAGCCTGGA GTCGTGCCGC AGCATGGTGA ACCTCATGGA TCGAGACGGT 
AATGGGAAAC TGGGCCTGGT GGAGTTCAAC ATCCTGTGGA ACCGCATCCG AAATTACCTG 
ACCATCTTCC GAAAGTTTGA CCTGGACAAG TCTGGCAGCA TGAGTGCTTA TGAGATGAGG 
ATGGCCATCG AGGCTGCAGG CTTCAAGCTT AACAAGAAGC TGCATGAGCT CATCATCACC 
CACTACTCAG AGCCCGACCT GGCCGTGGAC TTTGACAACT TTGTGTGCTG CCTTGTGCGC 
CTGGAGACCA TGTTCCGGTT TTTCAAAATT CTGGACACAG ACCTGGATGG TGTTGTGACC 
TTTGATCTAT TTAAGTGGTT ACAGCTGACT ATGTTTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neuroprotective Effects of Oxytocin Hormone after an Experimental Stroke Model and the Possible Role of Calpain-1.
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association(2017 Dec)
Etehadi Moghadam S,Azami Tameh A,Vahidinia Z,Atlasi MA,Hassani Bafrani H,Naderian H

CaMKII inhibition mitigates ischemia/reperfusion-elicited calpain activation and the damage to membrane skeleton proteins in isolated rat hearts.
Biochemical and biophysical research communications491(3)687-692(2017 09)
Kong LH,Gu XM,Wu F,Jin ZX,Zhou JJ

Calpain-GRIP Signaling in Nucleus Accumbens Core Mediates the Reconsolidation of Drug Reward Memory.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(37)8938-8951(2017 Sep)
Liang J,Li JL,Han Y,Luo YX,Xue YX,Zhang Y,Zhang Y,Zhang LB,Chen ML,Lu L,Shi J

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Capn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報