Trdmt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trdmt1
Entrez Gene ID	291324
Full Name	tRNA aspartic acid methyltransferase 1
Synonyms	Dnmt2
General protein information	Preferred Names tRNA aspartic acid methyltransferase 1 Names tRNA (cytosine(38)-C(5))-methyltransferase DNA (cytosine-5)-methyltransferase-like protein 2 DNA methyltransferase 2 tRNA (cytosine-5-)-methyltransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001031643.1	NP_001026813.1	tRNA (cytosine(38)-C(5))-methyltransferase
XM_017600497.1	XP_017455986.1	tRNA (cytosine(38)-C(5))-methyltransferase isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TRDMT1	XP_848593.1
Pan troglodytes (chimpanzee)	TRDMT1	XP_001151840.2
Rattus norvegicus (Norway rat)	Trdmt1	NP_001026813.1
Gallus gallus (chicken)	TRDMT1	NP_001020002.1
Bos taurus (cattle)	TRDMT1	NP_861528.1
Mus musculus (house mouse)	Trdmt1	NP_034197.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004101	XP_312975.5
Homo sapiens (human)	TRDMT1	NP_004403.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012836	XP_306830.2
Arabidopsis thaliana (thale cress)	DNMT2	NP_568474.1
Xenopus tropicalis (tropical clawed frog)	trdmt1	NP_001004959.2
Danio rerio (zebrafish)	trdmt1	NP_001018153.1
Drosophila melanogaster (fruit fly)	Mt2	NP_001036355.1
Schizosaccharomyces pombe (fission yeast)	pmt1	NP_595687.1

Gene Ontology
Bibliography

Related articles in PubMed

Human DNMT2 methylates tRNA(Asp) molecules using a DNA methyltransferase-like catalytic mechanism.
Jurkowski TP, Meusburger M, Phalke S, Helm M, Nellen W, Reuter G, Jeltsch A
RNA (New York, N.Y.)14(8)1663-70(2008 Aug)

Methylation of tRNAAsp by the DNA methyltransferase homolog Dnmt2.
Goll MG, Kirpekar F, Maggert KA, Yoder JA, Hsieh CL, Zhang X, Golic KG, Jacobsen SE, Bestor TH
Science (New York, N.Y.)311(5759)395-8(2006 Jan)

Psychostimulant alters expression of DNA methyltransferase mRNA in the rat brain.
Numachi Y, Yoshida S, Yamashita M, Fujiyama K, Naka M, Matsuoka H, Sato M, Sora I
Annals of the New York Academy of Sciences1025102-9(2004 Oct)

The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity.
Hermann A, Schmitt S, Jeltsch A
The Journal of biological chemistry278(34)31717-21(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Trdmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trdmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08021	NM_001031643.1 Latest version!	Rattus norvegicus tRNA aspartic acid methyltransferase 1 (Trdmt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa59024	XM_017600497.1 Latest version!	Rattus norvegicus tRNA aspartic acid methyltransferase 1 (Trdmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trdmt1 ( NM_001031643.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001026813.1 Rattus norvegicus tRNA aspartic acid methyltransferase 1 (Trdmt1), mRNA.

Price & Availability↑

CloneID	ORa08021
Clone ID Related Accession (Same CDS sequence)	NM_001031643.1
Accession Version	NM_001031643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-15
Organism	Rattus norvegicus(Norway rat)
Product	tRNA (cytosine(38)-C(5))-methyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098700.1. On Aug 12, 2005 this sequence version replaced XM_214514.3. ##Evidence-Data-START## Transcript exon combination :: BC098700.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAACCTC TGCGTGTCCT GGAGCTGTAC AGTGGCATTG GGGGCATGCA CCACGCGCTG 
CGAGAAAGCC GTGTCCCTGC ACATGTGGTG GCTGCTATTG ACGTGAGCAC TGTTGCTAAC 
GAAGTATACA AGCATAACTT CCCTCATACA CACTTACTGG CAAAGACAAT CGAAGGTATT 
TCACTGGAAG AGTTTGACAA GCTATCTTTC AATATGATTT TAATGAGCCC TCCATGTCAG 
CCATTCACAA GAATTGGCCT ACAGGGGGAC ATGAGTGATC GAAGGACAAA TAGCTTCTTG 
TATATTCTAG ATATTCTCCC AAGGTTACAG AAGTTACCCA AGTATATTCT TTTAGAAAAT 
GTCAAAGGGT TTGAAGTTTC TTCTACAAGA GGGCTGCTGA TACAAACAAT GGAAGCCTGC 
GGCTTTCAGT ATCAAGAGTT TCTGTTGTCT CCGTCCTCTC TAGGCATTCC AAACTCAAGG 
CTACGGTATT TTCTCATTGC GAAGCTTCAG TCAGAGCCTC TCTGTTTCCA GGCCCCTGGC 
CAGATACTGA TGGAGTTTCC TAATAGTGGA ACAGTTCAGC CACAAGAATA TGCAGTAGTT 
GAAGAAGGCA AGCTGAGAGT GAGGACAAGG GAGCCAGATG TCTGCCTTGA TAGCAGCAGC 
ACCCAGTGCT CTGGACAAGA TAGCATTCTT TTTAAGCACG AGACTGCAGC AGACATTGAC 
AGGAAGCGCC AACAGGACAG TGACCTCTCT GTGCAGATGC TGAAAGGTTT CCTTGAAGAT 
GGTGACACAG CTCAGTACCT TCTGCCAGCG AAGTCGCTGC TGCGCTACGC TCTCCTCCTC 
GACATCGTTA AGCCCACCTC CAGGAGGTCC ATGTGCTTCA CTAAAGGGTA TGGAAGCTAC 
ATAGAAGGGA CAGGCTCTGT GTTACAGACT GCAGAGGATG TGCAGATTGA GAATATCTAC 
AAATCTCTTC CTGATTTGCC ACCAGAAGAG AAGATAGCTA AATTGTCAAT GCTTAAACTG 
CGATATTTCA CACCTAAAGA AATTGCAAAT CTCCTGGGAT TTCCTCCAGA ATTTGGGTTT 
CCTGAGAAGA CAACGGTGAA GCAGCGTTAC CGGCTGCTCG GCAACAGCCT CAACGTGCAT 
GTCGTCTCAA AGCTCCTTAC AGTCCTGTGT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026813.1
CDS	35..1210
Translation	MEPLRVLELYSGIGGMHHALRESRVPAHVVAAIDVSTVANEVYKHNFPHTHLLAKTIEGISLEEFDKLSFNMILMSPPCQPFTRIGLQGDMSDRRTNSFLYILDILPRLQKLPKYILLENVKGFEVSSTRGLLIQTMEACGFQYQEFLLSPSSLGIPNSRLRYFLIAKLQSEPLCFQAPGQILMEFPNSGTVQPQEYAVVEEGKLRVRTREPDVCLDSSSTQCSGQDSILFKHETAADIDRKRQQDSDLSVQMLKGFLEDGDTAQYLLPAKSLLRYALLLDIVKPTSRRSMCFTKGYGSYIEGTGSVLQTAEDVQIENIYKSLPDLPPEEKIAKLSMLKLRYFTPKEIANLLGFPPEFGFPEKTTVKQRYRLLGNSLNVHVVSKLLTVLCE

Target ORF information:

RefSeq Version	NM_001031643.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tRNA aspartic acid methyltransferase 1 (Trdmt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031643.1

ORF Insert Sequence:

ATGGAACCTC TGCGTGTCCT GGAGCTGTAC AGTGGCATTG GGGGCATGCA CCACGCGCTG 
CGAGAAAGCC GTGTCCCTGC ACATGTGGTG GCTGCTATTG ACGTGAGCAC TGTTGCTAAC 
GAAGTATACA AGCATAACTT CCCTCATACA CACTTACTGG CAAAGACAAT CGAAGGTATT 
TCACTGGAAG AGTTTGACAA GCTATCTTTC AATATGATTT TAATGAGCCC TCCATGTCAG 
CCATTCACAA GAATTGGCCT ACAGGGGGAC ATGAGTGATC GAAGGACAAA TAGCTTCTTG 
TATATTCTAG ATATTCTCCC AAGGTTACAG AAGTTACCCA AGTATATTCT TTTAGAAAAT 
GTCAAAGGGT TTGAAGTTTC TTCTACAAGA GGGCTGCTGA TACAAACAAT GGAAGCCTGC 
GGCTTTCAGT ATCAAGAGTT TCTGTTGTCT CCGTCCTCTC TAGGCATTCC AAACTCAAGG 
CTACGGTATT TTCTCATTGC GAAGCTTCAG TCAGAGCCTC TCTGTTTCCA GGCCCCTGGC 
CAGATACTGA TGGAGTTTCC TAATAGTGGA ACAGTTCAGC CACAAGAATA TGCAGTAGTT 
GAAGAAGGCA AGCTGAGAGT GAGGACAAGG GAGCCAGATG TCTGCCTTGA TAGCAGCAGC 
ACCCAGTGCT CTGGACAAGA TAGCATTCTT TTTAAGCACG AGACTGCAGC AGACATTGAC 
AGGAAGCGCC AACAGGACAG TGACCTCTCT GTGCAGATGC TGAAAGGTTT CCTTGAAGAT 
GGTGACACAG CTCAGTACCT TCTGCCAGCG AAGTCGCTGC TGCGCTACGC TCTCCTCCTC 
GACATCGTTA AGCCCACCTC CAGGAGGTCC ATGTGCTTCA CTAAAGGGTA TGGAAGCTAC 
ATAGAAGGGA CAGGCTCTGT GTTACAGACT GCAGAGGATG TGCAGATTGA GAATATCTAC 
AAATCTCTTC CTGATTTGCC ACCAGAAGAG AAGATAGCTA AATTGTCAAT GCTTAAACTG 
CGATATTTCA CACCTAAAGA AATTGCAAAT CTCCTGGGAT TTCCTCCAGA ATTTGGGTTT 
CCTGAGAAGA CAACGGTGAA GCAGCGTTAC CGGCTGCTCG GCAACAGCCT CAACGTGCAT 
GTCGTCTCAA AGCTCCTTAC AGTCCTGTGT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trdmt1 ( XM_017600497.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455986.1 Rattus norvegicus tRNA aspartic acid methyltransferase 1 (Trdmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa59024
Clone ID Related Accession (Same CDS sequence)	XM_017600497.1
Accession Version	XM_017600497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tRNA (cytosine(38)-C(5))-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTAA TGAGCCCTCC ATGTCAGCCA TTCACAAGAA TTGGCCTACA GGGGGACATG 
AGTGATCGAA GGACAAATAG CTTCTTGTAT ATTCTAGATA TTCTCCCAAG GTTACAGAAG 
TTACCCAAGT ATATTCTTTT AGAAAATGTC AAAGGGTTTG AAGTTTCTTC TACAAGAGGG 
CTGCTGATAC AAACAATGGA AGCCTGCGGC TTTCAGTATC AAGAGTTTCT GTTGTCTCCG 
TCCTCTCTAG GCATTCCAAA CTCAAGGCTA CGGTATTTTC TCATTGCGAA GCTTCAGTCA 
GAGCCTCTCT GTTTCCAGGC CCCTGGCCAG ATACTGATGG AGTTTCCTAA TAGTGGAACA 
GTTCAGCCAC AAGAATATGC AGTAGTTGAA GAAGGCAAGC TGAGAGTGAG GACAAGGGAG 
CCAGATGTCT GCCTTGATAG CAGCAGCACC CAGTGCTCTG GACAAGATAG CATTCTTTTT 
AAGCACGAGA CTGCAGCAGA CATTGACAGG AAGCGCCAAC AGGACAGTGA CCTCTCTGTG 
CAGATGCTGA AAGGTTTCCT TGAAGATGGT GACACAGCTC AGTACCTTCT GCCAGCGAAG 
TCGCTGCTGC GCTACGCTCT CCTCCTCGAC ATCGTTAAGC CCACCTCCAG GAGGTCCATG 
TGCTTCACTA AAGGGTATGG AAGCTACATA GAAGGGACAG GCTCTGTGTT ACAGACTGCA 
GAGGATGTGC AGATTGAGAA TATCTACAAA TCTCTTCCTG ATTTGCCACC AGAAGAGAAG 
ATAGCTAAAT TGTCAATGCT TAAACTGCGA TATTTCACAC CTAAAGAAAT TGCAAATCTC 
CTGGGATTTC CTCCAGAATT TGGGTTTCCT GAGAAGACAA CGGTGAAGCA GCGTTACCGG 
CTGCTCGGCA ACAGCCTCAA CGTGCATGTC GTCTCAAAGC TCCTTACAGT CCTGTGTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455986.1
CDS	3015..3977
Translation	MILMSPPCQPFTRIGLQGDMSDRRTNSFLYILDILPRLQKLPKYILLENVKGFEVSSTRGLLIQTMEACGFQYQEFLLSPSSLGIPNSRLRYFLIAKLQSEPLCFQAPGQILMEFPNSGTVQPQEYAVVEEGKLRVRTREPDVCLDSSSTQCSGQDSILFKHETAADIDRKRQQDSDLSVQMLKGFLEDGDTAQYLLPAKSLLRYALLLDIVKPTSRRSMCFTKGYGSYIEGTGSVLQTAEDVQIENIYKSLPDLPPEEKIAKLSMLKLRYFTPKEIANLLGFPPEFGFPEKTTVKQRYRLLGNSLNVHVVSKLLTVLCE

Target ORF information:

RefSeq Version	XM_017600497.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tRNA aspartic acid methyltransferase 1 (Trdmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600497.1

ORF Insert Sequence:

ATGATTTTAA TGAGCCCTCC ATGTCAGCCA TTCACAAGAA TTGGCCTACA GGGGGACATG 
AGTGATCGAA GGACAAATAG CTTCTTGTAT ATTCTAGATA TTCTCCCAAG GTTACAGAAG 
TTACCCAAGT ATATTCTTTT AGAAAATGTC AAAGGGTTTG AAGTTTCTTC TACAAGAGGG 
CTGCTGATAC AAACAATGGA AGCCTGCGGC TTTCAGTATC AAGAGTTTCT GTTGTCTCCG 
TCCTCTCTAG GCATTCCAAA CTCAAGGCTA CGGTATTTTC TCATTGCGAA GCTTCAGTCA 
GAGCCTCTCT GTTTCCAGGC CCCTGGCCAG ATACTGATGG AGTTTCCTAA TAGTGGAACA 
GTTCAGCCAC AAGAATATGC AGTAGTTGAA GAAGGCAAGC TGAGAGTGAG GACAAGGGAG 
CCAGATGTCT GCCTTGATAG CAGCAGCACC CAGTGCTCTG GACAAGATAG CATTCTTTTT 
AAGCACGAGA CTGCAGCAGA CATTGACAGG AAGCGCCAAC AGGACAGTGA CCTCTCTGTG 
CAGATGCTGA AAGGTTTCCT TGAAGATGGT GACACAGCTC AGTACCTTCT GCCAGCGAAG 
TCGCTGCTGC GCTACGCTCT CCTCCTCGAC ATCGTTAAGC CCACCTCCAG GAGGTCCATG 
TGCTTCACTA AAGGGTATGG AAGCTACATA GAAGGGACAG GCTCTGTGTT ACAGACTGCA 
GAGGATGTGC AGATTGAGAA TATCTACAAA TCTCTTCCTG ATTTGCCACC AGAAGAGAAG 
ATAGCTAAAT TGTCAATGCT TAAACTGCGA TATTTCACAC CTAAAGAAAT TGCAAATCTC 
CTGGGATTTC CTCCAGAATT TGGGTTTCCT GAGAAGACAA CGGTGAAGCA GCGTTACCGG 
CTGCTCGGCA ACAGCCTCAA CGTGCATGTC GTCTCAAAGC TCCTTACAGT CCTGTGTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human DNMT2 methylates tRNA(Asp) molecules using a DNA methyltransferase-like catalytic mechanism.
RNA (New York, N.Y.)14(8)1663-70(2008 Aug)
Jurkowski TP,Meusburger M,Phalke S,Helm M,Nellen W,Reuter G,Jeltsch A

Methylation of tRNAAsp by the DNA methyltransferase homolog Dnmt2.
Science (New York, N.Y.)311(5759)395-8(2006 Jan)
Goll MG,Kirpekar F,Maggert KA,Yoder JA,Hsieh CL,Zhang X,Golic KG,Jacobsen SE,Bestor TH

Psychostimulant alters expression of DNA methyltransferase mRNA in the rat brain.
Annals of the New York Academy of Sciences1025102-9(2004 Oct)
Numachi Y,Yoshida S,Yamashita M,Fujiyama K,Naka M,Matsuoka H,Sato M,Sora I

The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity.
The Journal of biological chemistry278(34)31717-21(2003 Aug)
Hermann A,Schmitt S,Jeltsch A

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Trdmt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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