Cers1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cers1
Entrez Gene ID	290658
Full Name	ceramide synthase 1
Synonyms	Lass1
General protein information	Preferred Names ceramide synthase 1 Names ceramide synthase 1 LAG1 homolog, ceramide synthase 1 LAG1 longevity assurance homolog 1 longevity assurance homolog 1 longevity assurance-like protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p14
Summary	This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. The orthologous human protein has been shown to regulate C18-ceramide (N-stearoyl-sphinganine) synthesis. The protein is expressed from a bicistronic mRNA which also encodes growth differentiation factor 1. [provided by RefSeq, Jul 2009].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044230.2	NP_001037695.1	ceramide synthase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LASS1	XP_524160.3
Homo sapiens (human)	CERS1	NP_067090.1
Gallus gallus (chicken)	CERS1	NP_001264694.1
Caenorhabditis elegans (roundworm)	lagr-1	NP_493403.1
Danio rerio (zebrafish)	cers1	XP_005161672.1
Bos taurus (cattle)	CERS1	NP_001076983.1
Mus musculus (house mouse)	Cers1	NP_619588.1
Rattus norvegicus (Norway rat)	Cers1	NP_001037695.1
Xenopus tropicalis (tropical clawed frog)	cers1	XP_002940648.1

Gene Ontology
Bibliography

Related articles in PubMed

Modulation of sphingolipid metabolism with calorie restriction enhances insulin action in skeletal muscle.
Obanda DN, Yu Y, Wang ZQ, Cefalu WT
The Journal of nutritional biochemistry26(7)687-95(2015 Jul)

Impairment of ceramide synthesis causes a novel progressive myoclonus epilepsy.
Vanni N, Fruscione F, Ferlazzo E, Striano P, Robbiano A, Traverso M, Sander T, Falace A, Gazzerro E, Bramanti P, Bielawski J, Fassio A, Minetti C, Genton P, Zara F
Annals of neurology76(2)206-12(2014 Aug)

Stress-induced ER to Golgi translocation of ceramide synthase 1 is dependent on proteasomal processing.
Sridevi P, Alexander H, Laviad EL, Min J, Mesika A, Hannink M, Futerman AH, Alexander S
Experimental cell research316(1)78-91(2010 Jan)

2-Hydroxy-ceramide synthesis by ceramide synthase family: enzymatic basis for the preference of FA chain length.
Mizutani Y, Kihara A, Chiba H, Tojo H, Igarashi Y
Journal of lipid research49(11)2356-64(2008 Nov)

(Dihydro)ceramide synthase 1 regulated sensitivity to cisplatin is associated with the activation of p38 mitogen-activated protein kinase and is abrogated by sphingosine kinase 1.
Min J, Mesika A, Sivaguru M, Van Veldhoven PP, Alexander H, Futerman AH, Alexander S
Molecular cancer research : MCR5(8)801-12(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest expression of enzymes involved in sphingolipid metabolism in skeletal muscle are regulated by dietary factors; caloric restriction up-regulates expression of Cers1 and Cers2 but down-regulates serine palmitoyltransferases (Spt1, Spt2).
Title: Modulation of sphingolipid metabolism with calorie restriction enhances insulin action in skeletal muscle.

Cloning and sequence analysis revealed that this transcript contains two nonoverlapping open reading frames (ORFs), LASS1 and GDF1, which are quite different to the predicted sequences in GenBank (accession number XM_224734 and XM_224733).
Title: Cloning and characterization of a LASS1-GDF1 transcript in rat cerebral cortex: conservation of a bicistronic structure.

The following Cers1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cers1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09907	NM_001044230.2 Latest version!	Rattus norvegicus ceramide synthase 1 (Cers1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cers1 ( NM_001044230.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001037695.1 Rattus norvegicus ceramide synthase 1 (Cers1), mRNA.

Price & Availability↑

CloneID	ORa09907
Clone ID Related Accession (Same CDS sequence)	NM_001044230.2
Accession Version	NM_001044230.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	ceramide synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC166409.1. On Jul 16, 2009 this sequence version replaced NM_001044230.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC166409.1, DQ479969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## bicistronic transcript :: PMID: 17364820 NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCTG CAGCGACGAC CACCGGGCTC GAGGCGCCAG AGCCCATGCC GAGTTATGCA 
GAGATGTTGC AACGAAGCTG GGCCTCGGCG CTGGCCGCAG CACGGGGCTG CGGGGACTGC 
GGCTGGGGAC TGGCGCGCCG CGGCCTGGCG GAGCACGCTC ATCTGGCTGC ACCCGAGCTG 
CTGCTGGCCG TGCTCTGCGC TCTGGGGTGG ACAGCGTTGC GCTCAGCAGC CACCACGCGC 
ATCTTTCGGC CCCTGGCCAA GCGGTGTCGC CTGCAGCCTA GAGACGCGGC TAGGCTGCCT 
GAGAGTGCCT GGAAGCTTCT GTTCTACTTG GCCTGTTGGA GCTACTGTGC CTACCTGCTC 
TTGGGCACCA GTTACCCTTT CTTCCATGAC CCGCCCTCTG TCTTCTACGG CTGGAGGTCG 
GGCATGGCGG TGCCCTGGGA CATAGCGGTC GCCTACCTGC TGCAGGGGAG TTTCTACTGT 
CATTCCGTCT ATGCCACCGT GTACATGGAC AGCTGGCGCA AGGACTCGGT GGTCATGCTG 
GTACACCATG TGGTCACCTT ACTGCTCATC GCCTCTTCCT ATGCGTTCCG ATACCACAAC 
GTGGGCCTCC TCGTGTTCTT CCTGCATGAC GTCAGCGATG TGCAGCTGGA GTTCACCAAG 
CTCAACATCT ACTTCAAGGC CAGGGGTGGC GCCTACCATC GATTGCACGG GCTGGTGGCC 
AACCTGGGCT GCCTCAGCTT CTGCTTCTGC TGGTTCTGGT TCCGCCTCTA CTGGTTCCCA 
CTCAAGGTTC TCTATGCCAC CTGGCACTGC AGCCTGCAGT CTGTGCCTGA CATTCCGTAC 
TATTTCTTCT TCAACACTCT GCTGCTGCTC CTCCTGGTCA TGAACATCTA CTGGTTCCTG 
TACATTGTGG CTTTTGCAGC CAAGGTGCTG ACCGGTCAGA TGCGTGAACT GGAAGACTTG 
CGGGAATACG ACAGTCTGGA AGCGCAGACA GCCAAGCCCT GCAGAGCTGA GAAGCCTCTA 
AGGAATGGCC TGGTGAAGGA CAAACTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037695.1
CDS	29..1081
Translation	MAAAATTTGLEAPEPMPSYAEMLQRSWASALAAARGCGDCGWGLARRGLAEHAHLAAPELLLAVLCALGWTALRSAATTRIFRPLAKRCRLQPRDAARLPESAWKLLFYLACWSYCAYLLLGTSYPFFHDPPSVFYGWRSGMAVPWDIAVAYLLQGSFYCHSVYATVYMDSWRKDSVVMLVHHVVTLLLIASSYAFRYHNVGLLVFFLHDVSDVQLEFTKLNIYFKARGGAYHRLHGLVANLGCLSFCFCWFWFRLYWFPLKVLYATWHCSLQSVPDIPYYFFFNTLLLLLLVMNIYWFLYIVAFAAKVLTGQMRELEDLREYDSLEAQTAKPCRAEKPLRNGLVKDKLF

Target ORF information:

RefSeq Version	NM_001044230.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ceramide synthase 1 (Cers1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044230.2

ORF Insert Sequence:

ATGGCTGCTG CAGCGACGAC CACCGGGCTC GAGGCGCCAG AGCCCATGCC GAGTTATGCA 
GAGATGTTGC AACGAAGCTG GGCCTCGGCG CTGGCCGCAG CACGGGGCTG CGGGGACTGC 
GGCTGGGGAC TGGCGCGCCG CGGCCTGGCG GAGCACGCTC ATCTGGCTGC ACCCGAGCTG 
CTGCTGGCCG TGCTCTGCGC TCTGGGGTGG ACAGCGTTGC GCTCAGCAGC CACCACGCGC 
ATCTTTCGGC CCCTGGCCAA GCGGTGTCGC CTGCAGCCTA GAGACGCGGC TAGGCTGCCT 
GAGAGTGCCT GGAAGCTTCT GTTCTACTTG GCCTGTTGGA GCTACTGTGC CTACCTGCTC 
TTGGGCACCA GTTACCCTTT CTTCCATGAC CCGCCCTCTG TCTTCTACGG CTGGAGGTCG 
GGCATGGCGG TGCCCTGGGA CATAGCGGTC GCCTACCTGC TGCAGGGGAG TTTCTACTGT 
CATTCCGTCT ATGCCACCGT GTACATGGAC AGCTGGCGCA AGGACTCGGT GGTCATGCTG 
GTACACCATG TGGTCACCTT ACTGCTCATC GCCTCTTCCT ATGCGTTCCG ATACCACAAC 
GTGGGCCTCC TCGTGTTCTT CCTGCATGAC GTCAGCGATG TGCAGCTGGA GTTCACCAAG 
CTCAACATCT ACTTCAAGGC CAGGGGTGGC GCCTACCATC GATTGCACGG GCTGGTGGCC 
AACCTGGGCT GCCTCAGCTT CTGCTTCTGC TGGTTCTGGT TCCGCCTCTA CTGGTTCCCA 
CTCAAGGTTC TCTATGCCAC CTGGCACTGC AGCCTGCAGT CTGTGCCTGA CATTCCGTAC 
TATTTCTTCT TCAACACTCT GCTGCTGCTC CTCCTGGTCA TGAACATCTA CTGGTTCCTG 
TACATTGTGG CTTTTGCAGC CAAGGTGCTG ACCGGTCAGA TGCGTGAACT GGAAGACTTG 
CGGGAATACG ACAGTCTGGA AGCGCAGACA GCCAAGCCCT GCAGAGCTGA GAAGCCTCTA 
AGGAATGGCC TGGTGAAGGA CAAACTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Modulation of sphingolipid metabolism with calorie restriction enhances insulin action in skeletal muscle.
The Journal of nutritional biochemistry26(7)687-95(2015 Jul)
Obanda DN,Yu Y,Wang ZQ,Cefalu WT

Impairment of ceramide synthesis causes a novel progressive myoclonus epilepsy.
Annals of neurology76(2)206-12(2014 Aug)
Vanni N,Fruscione F,Ferlazzo E,Striano P,Robbiano A,Traverso M,Sander T,Falace A,Gazzerro E,Bramanti P,Bielawski J,Fassio A,Minetti C,Genton P,Zara F

Stress-induced ER to Golgi translocation of ceramide synthase 1 is dependent on proteasomal processing.
Experimental cell research316(1)78-91(2010 Jan)
Sridevi P,Alexander H,Laviad EL,Min J,Mesika A,Hannink M,Futerman AH,Alexander S

2-Hydroxy-ceramide synthesis by ceramide synthase family: enzymatic basis for the preference of FA chain length.
Journal of lipid research49(11)2356-64(2008 Nov)
Mizutani Y,Kihara A,Chiba H,Tojo H,Igarashi Y

(Dihydro)ceramide synthase 1 regulated sensitivity to cisplatin is associated with the activation of p38 mitogen-activated protein kinase and is abrogated by sphingosine kinase 1.
Molecular cancer research : MCR5(8)801-12(2007 Aug)
Min J,Mesika A,Sivaguru M,Van Veldhoven PP,Alexander H,Futerman AH,Alexander S

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Cers1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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