Osgep cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Osgep
Entrez Gene ID	290028
Full Name	O-sialoglycoprotein endopeptidase
General protein information	Preferred Names O-sialoglycoprotein endopeptidase Names probable tRNA N6-adenosine threonylcarbamoyltransferase N6-L-threonylcarbamoyladenine synthase O-sialoglycoprotease Prsmg1/Gcpl1 pasteurella haemolytica metalloprotease homolog with glycoprotein substrates/gpc-like protein 1 probable O-sialoglycoprotein endopeptidase probable tRNA threonylcarbamoyladenosine biosynthesis protein Osgep t(6)A synthase t(6)A37 threonylcarbamoyladenosine biosynthesis protein Osgep tRNA threonylcarbamoyladenosine biosynthesis protein Osgep
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p14

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100510.1	NP_001093980.1	probable tRNA N6-adenosine threonylcarbamoyltransferase

Pathways from BioSystems

Homologies

Homo sapiens (human)	OSGEP	NP_060277.1
Canis lupus familiaris (dog)	OSGEP	XP_003432020.2
Danio rerio (zebrafish)	osgep	NP_001017751.2
Caenorhabditis elegans (roundworm)	Y71H2AM.1	NP_497625.3
Kluyveromyces lactis	KLLA0F21450g	XP_456041.1
Schizosaccharomyces pombe (fission yeast)	pgp2	NP_596498.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006952	XP_308804.2
Arabidopsis thaliana (thale cress)	AT4G22720	NP_974593.1
Xenopus tropicalis (tropical clawed frog)	osgep	NP_001016112.1
	OSGEP	XP_001088971.1
Bos taurus (cattle)	OSGEP	NP_001068787.1
Saccharomyces cerevisiae (baker's yeast)	KAE1	NP_012964.2
Pan troglodytes (chimpanzee)	OSGEP	XP_001139005.1
Rattus norvegicus (Norway rat)	Osgep	NP_001093980.1
Magnaporthe oryzae (rice blast fungus)	MGG_06931	XP_003709670.1
Mus musculus (house mouse)	Osgep	NP_598437.2
Drosophila melanogaster (fruit fly)	CG4933	NP_648880.1
Eremothecium gossypii	AGOS_AEL316W	NP_984544.1
Neurospora crassa	NCU03836	XP_960595.1
Oryza sativa (rice)	Os05g0194600	NP_001054858.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly.
Braun DA, Rao J, Mollet G, Schapiro D, Daugeron MC, Tan W, Gribouval O, Boyer O, Revy P, Jobst-Schwan T, Schmidt JM, Lawson JA, Schanze D, Ashraf S, Ullmann JFP, Hoogstraten CA, Boddaert N, Collinet B, Martin G, Liger D, Lovric S, Furlano M, Guerrera IC, Sanchez-Ferras O, Hu JF, Boschat AC, Sanquer S, Menten B, Vergult S, De Rocker N, Airik M, Hermle T, Shril S, Widmeier E, Gee HY, Choi WI, Sadowski CE, Pabst WL, Warejko JK, Daga A, Basta T, Matejas V, Scharmann K, Kienast SD, Behnam B, Beeson B, Begtrup A, Bruce M, Ch'ng GS, Lin SP, Chang JH, Chen CH, Cho MT, Gaffney PM, Gipson PE, Hsu CH, Kari JA, Ke YY, Kiraly-Borri C, Lai WM, Lemyre E, Littlejohn RO, Masri A, Moghtaderi M, Nakamura K, Ozaltin F, Praet M, Prasad C, Prytula A, Roeder ER, Rump P, Schnur RE, Shiihara T, Sinha MD, Soliman NA, Soulami K, Sweetser DA, Tsai WH, Tsai JD, Topaloglu R, Vester U, Viskochil DH, Vatanavicharn N, Waxler JL, Wierenga KJ, Wolf MTF, Wong SN, Leidel SA, Truglio G, Dedon PC, Poduri A, Mane S, Lifton RP, Bouchard M, Kannu P, Chitayat D, Magen D, Callewaert B, van Tilbeurgh H, Zenker M, Antignac C, Hildebrandt F
Nature genetics49(10)1529-1538(2017 Oct)

Proteomic analysis of the human KEOPS complex identifies C14ORF142 as a core subunit homologous to yeast Gon7.
Wan LC, Maisonneuve P, Szilard RK, Lambert JP, Ng TF, Manczyk N, Huang H, Laister R, Caudy AA, Gingras AC, Durocher D, Sicheri F
Nucleic acids research45(2)805-817(2017 Jan)

Genomic structure of the rat major AP endonuclease gene (Apex) with an adjacent putative O-sialoglycoprotease gene (Prsmg1/Gcpl1) and a processed Apex pseudogene (Apexp1).
Yao M, Akiyama K, Tan Y, Sarker AH, Ikeda S, Alam SS, Tsutsui K, Yoshida MC, Seki S
Acta medica Okayama53(6)245-52(1999 Dec)

Structure, promoter analysis and chromosomal assignment of the human APEX gene.
Akiyama K, Seki S, Oshida T, Yoshida MC
Biochimica et biophysica acta1219(1)15-25(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Osgep gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Osgep cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13661	NM_001100510.1 Latest version!	Rattus norvegicus O-sialoglycoprotein endopeptidase (Osgep), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Osgep ( NM_001100510.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001093980.1 Rattus norvegicus O-sialoglycoprotein endopeptidase (Osgep), mRNA.

Price & Availability↑

CloneID	ORa13661
Clone ID Related Accession (Same CDS sequence)	NM_001100510.1
Accession Version	NM_001100510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	probable tRNA N6-adenosine threonylcarbamoyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC158592.1. On or before Jun 4, 2008 this sequence version replaced XM_001064067.1, XM_214163.4. ##Evidence-Data-START## Transcript exon combination :: BC158592.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGCGG TGCTGGGGTT CGAAGGCAGC GCCAACAAGA TTGGCGTGGG CGTGGTCCGC 
GACGGCACGG TGCTGGCGAA CCCGCGGCGC ACATACGTCA CGGCCCCGGG CACCGGTTTC 
CTTCCAGGTG ACACGGCCAG GCACCACCGA GCTGTCATCC TAGACCTACT GCAGGAGGCG 
CTAACAGAGG CAGGGTTAAC CCCCAAGGAC ATCGATTGCA TTGCTTACAC CAAAGGTCCT 
GGCATGGGAG CCCCACTGGC TTCCGTAGCT GTTGTTGCCC GCACTGTGGC CCAGTTGTGG 
AATAAGCCTT TGCTAGGCGT GAACCACTGC ATAGGCCACA TTGAAATGGG CCGCCTCATC 
ACTGGAGCCG TCAACCCAAC TGTCTTGTAT GTCAGCGGAG GAAATACTCA GGTGATTTCC 
TACTCAGAAC ATCGTTACCG CATCTTTGGG GAAACTATTG ATATTGCTGT GGGAAATTGT 
TTGGATCGTT TTGCCCGGGT GCTGAAGATT TCCAATGACC CAAGTCCAGG CTACAACATT 
GAGCAGATGG CAAAGCGAGG CAAGAAGCTA GTGGAGCTGC CATACACCGT GAAGGGGATG 
GACGTCTCGT TCTCAGGGAT CCTGTCCTTC ATCGAGGATG CAGCACAGCG AATGCTGGCC 
ACGGGAGAGT GTACTCCTGA AGATCTGTGT TTCTCCTTAC AGGAGACTGT GTTTGCAATG 
CTGGTGGAGA TCACAGAGCG AGCCATGGCG CACTGTGGCT CCAAAGAAGC CCTCATCGTC 
GGGGGAGTTG GATGTAACGT ACGGCTGCAG GAGATGATGG CAACAATGTG CCAGGAGCGG 
GGAGCCCAGC TCTTTGCAAC AGATGAGAGG TTCTGCATTG ACAATGGAGC CATGATAGCA 
CAAGCTGGCT GGGAGATGTT TCAGGCTGGG CACAGGACCC CTCTCCAAGA TTCTGGGATC 
ACTCAGAGGT ATAGGACAGA TGAAGTAGAA GTGACGTGGA GGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093980.1
CDS	90..1097
Translation	MPAVLGFEGSANKIGVGVVRDGTVLANPRRTYVTAPGTGFLPGDTARHHRAVILDLLQEALTEAGLTPKDIDCIAYTKGPGMGAPLASVAVVARTVAQLWNKPLLGVNHCIGHIEMGRLITGAVNPTVLYVSGGNTQVISYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQMAKRGKKLVELPYTVKGMDVSFSGILSFIEDAAQRMLATGECTPEDLCFSLQETVFAMLVEITERAMAHCGSKEALIVGGVGCNVRLQEMMATMCQERGAQLFATDERFCIDNGAMIAQAGWEMFQAGHRTPLQDSGITQRYRTDEVEVTWRD

Target ORF information:

RefSeq Version	NM_001100510.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus O-sialoglycoprotein endopeptidase (Osgep), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100510.1

ORF Insert Sequence:

ATGCCCGCGG TGCTGGGGTT CGAAGGCAGC GCCAACAAGA TTGGCGTGGG CGTGGTCCGC 
GACGGCACGG TGCTGGCGAA CCCGCGGCGC ACATACGTCA CGGCCCCGGG CACCGGTTTC 
CTTCCAGGTG ACACGGCCAG GCACCACCGA GCTGTCATCC TAGACCTACT GCAGGAGGCG 
CTAACAGAGG CAGGGTTAAC CCCCAAGGAC ATCGATTGCA TTGCTTACAC CAAAGGTCCT 
GGCATGGGAG CCCCACTGGC TTCCGTAGCT GTTGTTGCCC GCACTGTGGC CCAGTTGTGG 
AATAAGCCTT TGCTAGGCGT GAACCACTGC ATAGGCCACA TTGAAATGGG CCGCCTCATC 
ACTGGAGCCG TCAACCCAAC TGTCTTGTAT GTCAGCGGAG GAAATACTCA GGTGATTTCC 
TACTCAGAAC ATCGTTACCG CATCTTTGGG GAAACTATTG ATATTGCTGT GGGAAATTGT 
TTGGATCGTT TTGCCCGGGT GCTGAAGATT TCCAATGACC CAAGTCCAGG CTACAACATT 
GAGCAGATGG CAAAGCGAGG CAAGAAGCTA GTGGAGCTGC CATACACCGT GAAGGGGATG 
GACGTCTCGT TCTCAGGGAT CCTGTCCTTC ATCGAGGATG CAGCACAGCG AATGCTGGCC 
ACGGGAGAGT GTACTCCTGA AGATCTGTGT TTCTCCTTAC AGGAGACTGT GTTTGCAATG 
CTGGTGGAGA TCACAGAGCG AGCCATGGCG CACTGTGGCT CCAAAGAAGC CCTCATCGTC 
GGGGGAGTTG GATGTAACGT ACGGCTGCAG GAGATGATGG CAACAATGTG CCAGGAGCGG 
GGAGCCCAGC TCTTTGCAAC AGATGAGAGG TTCTGCATTG ACAATGGAGC CATGATAGCA 
CAAGCTGGCT GGGAGATGTT TCAGGCTGGG CACAGGACCC CTCTCCAAGA TTCTGGGATC 
ACTCAGAGGT ATAGGACAGA TGAAGTAGAA GTGACGTGGA GGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly.
Nature genetics49(10)1529-1538(2017 Oct)
Braun DA,Rao J,Mollet G,Schapiro D,Daugeron MC,Tan W,Gribouval O,Boyer O,Revy P,Jobst-Schwan T,Schmidt JM,Lawson JA,Schanze D,Ashraf S,Ullmann JFP,Hoogstraten CA,Boddaert N,Collinet B,Martin G,Liger D,Lovric S,Furlano M,Guerrera IC,Sanchez-Ferras O,Hu JF,Boschat AC,Sanquer S,Menten B,Vergult S,De Rocker N,Airik M,Hermle T,Shril S,Widmeier E,Gee HY,Choi WI,Sadowski CE,Pabst WL,Warejko JK,Daga A,Basta T,Matejas V,Scharmann K,Kienast SD,Behnam B,Beeson B,Begtrup A,Bruce M,Ch'ng GS,Lin SP,Chang JH,Chen CH,Cho MT,Gaffney PM,Gipson PE,Hsu CH,Kari JA,Ke YY,Kiraly-Borri C,Lai WM,Lemyre E,Littlejohn RO,Masri A,Moghtaderi M,Nakamura K,Ozaltin F,Praet M,Prasad C,Prytula A,Roeder ER,Rump P,Schnur RE,Shiihara T,Sinha MD,Soliman NA,Soulami K,Sweetser DA,Tsai WH,Tsai JD,Topaloglu R,Vester U,Viskochil DH,Vatanavicharn N,Waxler JL,Wierenga KJ,Wolf MTF,Wong SN,Leidel SA,Truglio G,Dedon PC,Poduri A,Mane S,Lifton RP,Bouchard M,Kannu P,Chitayat D,Magen D,Callewaert B,van Tilbeurgh H,Zenker M,Antignac C,Hildebrandt F

Proteomic analysis of the human KEOPS complex identifies C14ORF142 as a core subunit homologous to yeast Gon7.
Nucleic acids research45(2)805-817(2017 Jan)
Wan LC,Maisonneuve P,Szilard RK,Lambert JP,Ng TF,Manczyk N,Huang H,Laister R,Caudy AA,Gingras AC,Durocher D,Sicheri F

Genomic structure of the rat major AP endonuclease gene (Apex) with an adjacent putative O-sialoglycoprotease gene (Prsmg1/Gcpl1) and a processed Apex pseudogene (Apexp1).
Acta medica Okayama53(6)245-52(1999 Dec)
Yao M,Akiyama K,Tan Y,Sarker AH,Ikeda S,Alam SS,Tsutsui K,Yoshida MC,Seki S

Structure, promoter analysis and chromosomal assignment of the human APEX gene.
Biochimica et biophysica acta1219(1)15-25(1994 Sep)
Akiyama K,Seki S,Oshida T,Yoshida MC

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Osgep cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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