Chfr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chfr
Entrez Gene ID	288734
Full Name	checkpoint with forkhead and ring finger domains
General protein information	Preferred Names checkpoint with forkhead and ring finger domains Names E3 ubiquitin-protein ligase CHFR checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009258.1	NP_001009258.1	E3 ubiquitin-protein ligase CHFR
XM_017598303.1	XP_017453792.1	E3 ubiquitin-protein ligase CHFR isoform X3
XM_006249536.3	XP_006249598.1	E3 ubiquitin-protein ligase CHFR isoform X2
XM_006249535.3	XP_006249597.1	E3 ubiquitin-protein ligase CHFR isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Chfr	NP_001009258.1
Xenopus tropicalis (tropical clawed frog)	chfr	NP_989378.1
	CHFR	XP_001083796.2
Oryza sativa (rice)	Os11g0175500	NP_001065879.2
Homo sapiens (human)	CHFR	NP_001154816.1
Pan troglodytes (chimpanzee)	CHFR	XP_003314094.2
Bos taurus (cattle)	CHFR	NP_001192403.1
Danio rerio (zebrafish)	chfr	NP_001093485.1
Canis lupus familiaris (dog)	CHFR	XP_534631.3
Arabidopsis thaliana (thale cress)	AT1G47570	NP_001077687.1
Mus musculus (house mouse)	Chfr	NP_766305.2
Gallus gallus (chicken)	CHFR	XP_004945549.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures and Mechanisms of Enzymes Employed in the Synthesis and Degradation of PARP-Dependent Protein ADP-Ribosylation.
Barkauskaite E, Jankevicius G, Ahel I
Molecular cell58(6)935-46(2015 Jun)

Insulin-induced cell division is controlled by the adaptor Grb14 in a Chfr-dependent manner.
Perdereau D, Cailliau K, Browaeys-Poly E, Lescuyer A, Carr? N, Benhamed F, Goenaga D, Burnol AF
Cellular signalling27(4)798-806(2015 Apr)

SRG3/mBAF155 stabilizes the SWI/SNF-like BAF complex by blocking CHFR mediated ubiquitination and degradation of its major components.
Jung I, Sohn DH, Choi J, Kim JM, Jeon S, Seol JH, Seong RH
Biochemical and biophysical research communications418(3)512-7(2012 Feb)

Chfr is linked to tumour metastasis through the downregulation of HDAC1.
Oh YM, Kwon YE, Kim JM, Bae SJ, Lee BK, Yoo SJ, Chung CH, Deshaies RJ, Seol JH
Nature cell biology11(3)295-302(2009 Mar)

Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins.
Ahel I, Ahel D, Matsusaka T, Clark AJ, Pines J, Boulton SJ, West SC
Nature451(7174)81-5(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results show that Chfr and Grb14 collaborate in a negative feedback loop controlling insulin-stimulated cell division.
Title: Insulin-induced cell division is controlled by the adaptor Grb14 in a Chfr-dependent manner.

The following Chfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00625	NM_001009258.1 Latest version!	Rattus norvegicus checkpoint with forkhead and ring finger domains (Chfr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa35154	XM_006249535.3 Latest version!	Rattus norvegicus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (Chfr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa35155	XM_006249536.3 Latest version!	Rattus norvegicus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (Chfr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa57798	XM_017598303.1 Latest version!	Rattus norvegicus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (Chfr), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chfr ( NM_001009258.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009258.1 Rattus norvegicus checkpoint with forkhead and ring finger domains (Chfr), mRNA.

Price & Availability↑

CloneID	ORa00625
Clone ID Related Accession (Same CDS sequence)	NM_001009258.1
Accession Version	NM_001009258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase CHFR
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC087162.1. On Jan 7, 2005 this sequence version replaced XM_213807.2. ##Evidence-Data-START## Transcript exon combination :: BC087162.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCCAC ACGGGGAAGA CGAGCAGCCG CCACCGCGGG AGCCCTGGGG GAGGCTTCTT 
CGCCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTT CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA GAGTGGATGA AAAATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTAA TCAACAAACT GCGAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGTGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAC GAGCCAGAGC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA ATGGCAAACA GAGCTTAACT CAAGAATCCC TTGAAGCCAA TAAAGAAAAT 
ATGTTCCATG TGACCAAAGA ATCCTCAGGT CCAGGGCAGG GTGATGACCC CCAGGTTCCA 
CTCTTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTTC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCCT CTAGCTCTGG ACCTGGGACT ATAAGCATCT CCCCAAAAGA ATGTAGTTCA 
CTTGTTGCAA ATGGTGAACT CTCTAGCCTT TCTTCAGTTT TCCAAGACAA CGAAGCATCC 
TTTTCTTTGT TGAAAACCGA AGACACTGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AGCTTGACTT GAACCTCCAG TTATCAGTTG CAGACCAGCG TGGAATTGCC 
CAAACCTCAA GTGAGGATGT CAAAGATGCC TCTGTGAAAC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGTCA AGACCTTCTG CATGACTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT TGTGGAAGCA 
TACCTTCTCC AGCACCCAGA TAAGAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTTG ATAGTGAATC CTCAGACATC 
AGTCAGCCAT ACATTGTATG CAGACAGTGT CCTGAATACA GAAGGCAAGC AGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCCACAC AGGCCCTTGG TGGGGAAGCA 
CCTTCAACAT CTGCCAGCTT GACAACAGCC CCGGGTTATA CATGCCCTCT TCAGGGAAGC 
CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCGGACC GGAGAGCTGA GCATGAGCAG 
GATTCCCGCA TCGCCCCTCA ACAGTGTGCA GTGTGCCTGC AGCCCTTCTG CCACCTGTAC 
TGGGGCTGCA CGCGAACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG 
GGGGACAAGT GTTTGGATGG AGTGCTGAAC AATAACAATT ATGAGTCGGA TATCCTGAAG 
AATTACCTGA CAACCAGGGG TCTGACATGG AAAAATATGT TGACAGAGAG TCTCCTGGCT 
CTACAGCGGG GTGTATTTAT ACTGTCTGAT TACAGAATCA CTGGAAACAC CGTGCTGTGT 
TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTATC AGTATCGTCA GAACATTCCT 
GCTTCAGAGT TGCCAGTGAC CGTAACATCC CGTCCTGATT GCTACTGGGG CCGCAACTGT 
CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG 
TTCAAGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009258.1
CDS	55..2046
Translation	MEPHGEDEQPPPREPWGRLLRLGAEEDEPQILLWKREWTIGRRRGCDLSFPSNKLVSGDHCKLRVDEKSGEVTLEDTSTNGTVINKLRVVKKQTYPLQSGDIIYLVYRKNEPEHNVAYLYESLNGKQSLTQESLEANKENMFHVTKESSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGTISISPKECSSLVANGELSSLSSVFQDNEASFSLLKTEDTEELEPAKKKMKGDGELDLNLQLSVADQRGIAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLLQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATQALGGEAPSTSASLTTAPGYTCPLQGSHAICTCCFQPMPDRRAEHEQDSRIAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLTTRGLTWKNMLTESLLALQRGVFILSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001009258.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus checkpoint with forkhead and ring finger domains (Chfr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009258.1

ORF Insert Sequence:

ATGGAGCCAC ACGGGGAAGA CGAGCAGCCG CCACCGCGGG AGCCCTGGGG GAGGCTTCTT 
CGCCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTT CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA GAGTGGATGA AAAATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTAA TCAACAAACT GCGAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGTGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAC GAGCCAGAGC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA ATGGCAAACA GAGCTTAACT CAAGAATCCC TTGAAGCCAA TAAAGAAAAT 
ATGTTCCATG TGACCAAAGA ATCCTCAGGT CCAGGGCAGG GTGATGACCC CCAGGTTCCA 
CTCTTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTTC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCCT CTAGCTCTGG ACCTGGGACT ATAAGCATCT CCCCAAAAGA ATGTAGTTCA 
CTTGTTGCAA ATGGTGAACT CTCTAGCCTT TCTTCAGTTT TCCAAGACAA CGAAGCATCC 
TTTTCTTTGT TGAAAACCGA AGACACTGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AGCTTGACTT GAACCTCCAG TTATCAGTTG CAGACCAGCG TGGAATTGCC 
CAAACCTCAA GTGAGGATGT CAAAGATGCC TCTGTGAAAC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGTCA AGACCTTCTG CATGACTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT TGTGGAAGCA 
TACCTTCTCC AGCACCCAGA TAAGAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTTG ATAGTGAATC CTCAGACATC 
AGTCAGCCAT ACATTGTATG CAGACAGTGT CCTGAATACA GAAGGCAAGC AGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCCACAC AGGCCCTTGG TGGGGAAGCA 
CCTTCAACAT CTGCCAGCTT GACAACAGCC CCGGGTTATA CATGCCCTCT TCAGGGAAGC 
CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCGGACC GGAGAGCTGA GCATGAGCAG 
GATTCCCGCA TCGCCCCTCA ACAGTGTGCA GTGTGCCTGC AGCCCTTCTG CCACCTGTAC 
TGGGGCTGCA CGCGAACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG 
GGGGACAAGT GTTTGGATGG AGTGCTGAAC AATAACAATT ATGAGTCGGA TATCCTGAAG 
AATTACCTGA CAACCAGGGG TCTGACATGG AAAAATATGT TGACAGAGAG TCTCCTGGCT 
CTACAGCGGG GTGTATTTAT ACTGTCTGAT TACAGAATCA CTGGAAACAC CGTGCTGTGT 
TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTATC AGTATCGTCA GAACATTCCT 
GCTTCAGAGT TGCCAGTGAC CGTAACATCC CGTCCTGATT GCTACTGGGG CCGCAACTGT 
CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG 
TTCAAGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( XM_006249535.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249597.1 Rattus norvegicus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (Chfr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35154
Clone ID Related Accession (Same CDS sequence)	XM_006249535.3
Accession Version	XM_006249535.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase CHFR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249535.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAC ACGGGGAAGA CGAGCAGCCG CCACCGCGGG AGCCCTGGGG GAGGCTTCTT 
CGCCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTT CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA GAGTGGATGA AAAATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTAA TCAACAAACT GCGAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGTGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAC GAGCCAGAGC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA ATGGCAAACA GAGCTTAACT CAAGAATCCC TTGAAGCCAA TAAAGAAAAT 
ATGTTCCATG TGACCAAAGA ATCCTCAGGT CCAGGGCAGG GTGATGACCC CCAGGTTCCA 
CTCTTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTTC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCCT CTAGCTCTGG ACCTGGGACT ATAAGCATCT CCCCAAAAGA ATGTAGTTCA 
CTTGTTGCAA ATGGTGAACT CTCTAGCCTT TCTTCAGTTT TCCAAGACAA CGAAGCATCC 
TTTTCTTTGT TGAAAACCGA AGACACTGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AGCTTGACTT GAACCTCCAG TTATCAGTTG CAGACCAGCG TGGAATTGCC 
CAAACCTCAA GTGAGGATGT CAAAGATGCC TCTGTGAAAC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGTCA AGACCTTCTG CATGACTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT TGTGGAAGCA 
TACCTTCTCC AGCACCCAGA TAAGAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTTG ATAGTGAATC CTCAGACATC 
AGTCAGCCAT ACATTGTATG CAGACAGTGT CCTGAATACA GAAGGCAAGC AGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCCACAC AGGCCCTTGG TGGGGAAGCA 
CCTTCAACAT CTGCCAGCTT GACAACAGCA GCCCCGGGTT ATACATGCCC TCTTCAGGGA 
AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCGG ACCGGAGAGC TGAGCATGAG 
CAGGATTCCC GCATCGCCCC TCAACAGTGT GCAGTGTGCC TGCAGCCCTT CTGCCACCTG 
TACTGGGGCT GCACGCGAAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC 
CTGGGGGACA AGTGTTTGGA TGGAGTGCTG AACAATAACA ATTATGAGTC GGATATCCTG 
AAGAATTACC TGACAACCAG GGGTCTGACA TGGAAAAATA TGTTGACAGA GAGTCTCCTG 
GCTCTACAGC GGGGTGTATT TATACTGTCT GATTACAGAA TCACTGGAAA CACCGTGCTG 
TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ATCAGTATCG TCAGAACATT 
CCTGCTTCAG AGTTGCCAGT GACCGTAACA TCCCGTCCTG ATTGCTACTG GGGCCGCAAC 
TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA 
AGGTTCAAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249597.1
CDS	140..2134
Translation	MEPHGEDEQPPPREPWGRLLRLGAEEDEPQILLWKREWTIGRRRGCDLSFPSNKLVSGDHCKLRVDEKSGEVTLEDTSTNGTVINKLRVVKKQTYPLQSGDIIYLVYRKNEPEHNVAYLYESLNGKQSLTQESLEANKENMFHVTKESSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGTISISPKECSSLVANGELSSLSSVFQDNEASFSLLKTEDTEELEPAKKKMKGDGELDLNLQLSVADQRGIAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLLQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATQALGGEAPSTSASLTTAAPGYTCPLQGSHAICTCCFQPMPDRRAEHEQDSRIAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLTTRGLTWKNMLTESLLALQRGVFILSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_006249535.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (Chfr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249535.3

ORF Insert Sequence:

ATGGAGCCAC ACGGGGAAGA CGAGCAGCCG CCACCGCGGG AGCCCTGGGG GAGGCTTCTT 
CGCCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTT CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA GAGTGGATGA AAAATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTAA TCAACAAACT GCGAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGTGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAC GAGCCAGAGC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA ATGGCAAACA GAGCTTAACT CAAGAATCCC TTGAAGCCAA TAAAGAAAAT 
ATGTTCCATG TGACCAAAGA ATCCTCAGGT CCAGGGCAGG GTGATGACCC CCAGGTTCCA 
CTCTTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTTC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCCT CTAGCTCTGG ACCTGGGACT ATAAGCATCT CCCCAAAAGA ATGTAGTTCA 
CTTGTTGCAA ATGGTGAACT CTCTAGCCTT TCTTCAGTTT TCCAAGACAA CGAAGCATCC 
TTTTCTTTGT TGAAAACCGA AGACACTGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AGCTTGACTT GAACCTCCAG TTATCAGTTG CAGACCAGCG TGGAATTGCC 
CAAACCTCAA GTGAGGATGT CAAAGATGCC TCTGTGAAAC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGTCA AGACCTTCTG CATGACTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT TGTGGAAGCA 
TACCTTCTCC AGCACCCAGA TAAGAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTTG ATAGTGAATC CTCAGACATC 
AGTCAGCCAT ACATTGTATG CAGACAGTGT CCTGAATACA GAAGGCAAGC AGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCCACAC AGGCCCTTGG TGGGGAAGCA 
CCTTCAACAT CTGCCAGCTT GACAACAGCA GCCCCGGGTT ATACATGCCC TCTTCAGGGA 
AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCGG ACCGGAGAGC TGAGCATGAG 
CAGGATTCCC GCATCGCCCC TCAACAGTGT GCAGTGTGCC TGCAGCCCTT CTGCCACCTG 
TACTGGGGCT GCACGCGAAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC 
CTGGGGGACA AGTGTTTGGA TGGAGTGCTG AACAATAACA ATTATGAGTC GGATATCCTG 
AAGAATTACC TGACAACCAG GGGTCTGACA TGGAAAAATA TGTTGACAGA GAGTCTCCTG 
GCTCTACAGC GGGGTGTATT TATACTGTCT GATTACAGAA TCACTGGAAA CACCGTGCTG 
TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ATCAGTATCG TCAGAACATT 
CCTGCTTCAG AGTTGCCAGT GACCGTAACA TCCCGTCCTG ATTGCTACTG GGGCCGCAAC 
TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA 
AGGTTCAAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( XM_006249536.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249598.1 Rattus norvegicus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (Chfr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa35155
Clone ID Related Accession (Same CDS sequence)	XM_006249536.3
Accession Version	XM_006249536.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase CHFR isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249536.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAC ACGGGGAAGA CGAGCAGCCG CCACCGCGGG AGCCCTGGGG GAGGCTTCTT 
CGCCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTT CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA GAGTGGATGA AAAATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTAA TCAACAAACT GCGAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGTGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAC GAGCCAGAGC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA ATGGCAAACA GAGCTTAACT CAAGAATCCC TTGAAGCCAA TAAAGAAAAT 
ATGTTCCATG TGACCAAAGA ATCCTCAGGT CCAGGGCAGG GTGATGACCC CCAGGTTCCA 
CTCTTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTTC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCCT CTAGCTCTGG ACCTGGGACT ATAAGCATCT CCCCAAAAGA ATGTAGTTCA 
CTTGTTGCAA ATGGTGAACT CTCTAGCCTT TCTTCAGTTT TCCAAGACAA CGAAGCATCC 
TTTTCTTTGT TGAAAACCGA AGACACTGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AGCTTGACTT GAACCTCCAG TTATCAGTTG CAGACCAGCG TGGAATTGCC 
CAAACCTCAA GTGAGGATGT CAAAGATGCC TCTGTGAAAC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGTCA AGACCTTCTG CATGACTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT TGTGGAAGCA 
TACCTTCTCC AGCACCCAGA TAAGAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTTG ATAGTGAATC CTCAGACATC 
AGTCAGCCAT ACATTGTATG CAGACAGTGT CCTGAATACA GAAGGCAAGC AGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCCACAC AGGCCCTTGG TGGGGAAGCA 
CCTTCAACAT CTGCCAGCTT GACAACAGGT GCAGTGTGCC TGCAGCCCTT CTGCCACCTG 
TACTGGGGCT GCACGCGAAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC 
CTGGGGGACA AGTGTTTGGA TGGAGTGCTG AACAATAACA ATTATGAGTC GGATATCCTG 
AAGAATTACC TGACAACCAG GGGTCTGACA TGGAAAAATA TGTTGACAGA GAGTCTCCTG 
GCTCTACAGC GGGGTGTATT TATACTGTCT GATTACAGAA TCACTGGAAA CACCGTGCTG 
TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ATCAGTATCG TCAGAACATT 
CCTGCTTCAG AGTTGCCAGT GACCGTAACA TCCCGTCCTG ATTGCTACTG GGGCCGCAAC 
TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA 
AGGTTCAAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249598.1
CDS	140..2014
Translation	MEPHGEDEQPPPREPWGRLLRLGAEEDEPQILLWKREWTIGRRRGCDLSFPSNKLVSGDHCKLRVDEKSGEVTLEDTSTNGTVINKLRVVKKQTYPLQSGDIIYLVYRKNEPEHNVAYLYESLNGKQSLTQESLEANKENMFHVTKESSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGTISISPKECSSLVANGELSSLSSVFQDNEASFSLLKTEDTEELEPAKKKMKGDGELDLNLQLSVADQRGIAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLLQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATQALGGEAPSTSASLTTGAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLTTRGLTWKNMLTESLLALQRGVFILSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_006249536.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (Chfr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249536.3

ORF Insert Sequence:

ATGGAGCCAC ACGGGGAAGA CGAGCAGCCG CCACCGCGGG AGCCCTGGGG GAGGCTTCTT 
CGCCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTT CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA GAGTGGATGA AAAATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTAA TCAACAAACT GCGAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGTGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAC GAGCCAGAGC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA ATGGCAAACA GAGCTTAACT CAAGAATCCC TTGAAGCCAA TAAAGAAAAT 
ATGTTCCATG TGACCAAAGA ATCCTCAGGT CCAGGGCAGG GTGATGACCC CCAGGTTCCA 
CTCTTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTTC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCCT CTAGCTCTGG ACCTGGGACT ATAAGCATCT CCCCAAAAGA ATGTAGTTCA 
CTTGTTGCAA ATGGTGAACT CTCTAGCCTT TCTTCAGTTT TCCAAGACAA CGAAGCATCC 
TTTTCTTTGT TGAAAACCGA AGACACTGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AGCTTGACTT GAACCTCCAG TTATCAGTTG CAGACCAGCG TGGAATTGCC 
CAAACCTCAA GTGAGGATGT CAAAGATGCC TCTGTGAAAC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGTCA AGACCTTCTG CATGACTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT TGTGGAAGCA 
TACCTTCTCC AGCACCCAGA TAAGAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTTG ATAGTGAATC CTCAGACATC 
AGTCAGCCAT ACATTGTATG CAGACAGTGT CCTGAATACA GAAGGCAAGC AGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCCACAC AGGCCCTTGG TGGGGAAGCA 
CCTTCAACAT CTGCCAGCTT GACAACAGGT GCAGTGTGCC TGCAGCCCTT CTGCCACCTG 
TACTGGGGCT GCACGCGAAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC 
CTGGGGGACA AGTGTTTGGA TGGAGTGCTG AACAATAACA ATTATGAGTC GGATATCCTG 
AAGAATTACC TGACAACCAG GGGTCTGACA TGGAAAAATA TGTTGACAGA GAGTCTCCTG 
GCTCTACAGC GGGGTGTATT TATACTGTCT GATTACAGAA TCACTGGAAA CACCGTGCTG 
TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ATCAGTATCG TCAGAACATT 
CCTGCTTCAG AGTTGCCAGT GACCGTAACA TCCCGTCCTG ATTGCTACTG GGGCCGCAAC 
TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA 
AGGTTCAAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( XM_017598303.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453792.1 Rattus norvegicus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (Chfr), transcript variant X3, mRNA.

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CloneID	ORa57798
Clone ID Related Accession (Same CDS sequence)	XM_017598303.1
Accession Version	XM_017598303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase CHFR isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAC ACGGGGAAGA CGAGCAGCCG CCACCGCGGG AGCCCTGGGG GAGGCTTCTT 
CGCCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTT CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA GAGTGGATGA AAAATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTAA TCAACAAACT GCGAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGTGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAC GAGCCAGAGC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA ATGGCAAACA GAGCTTAACT CAAGAATCCC TTGAAGCCAA TAAAGAAAAT 
ATGTTCCATG TGACCAAAGA ATCCTCAGGT CCAGGGCAGG GTGATGACCC CCAGGTTCCA 
CTCTTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTTC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCCT CTAGCTCTGG ACCTGGGACT ATAAGCATCT CCCCAAAAGA ATGTAGTTCA 
CTTGTTGCAA ATGGTGAACT CTCTAGCCTT TCTTCAGTTT TCCAAGACAA CGAAGCATCC 
TTTTCTTTGT TGAAAACCGA AGACACTGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AGCTTGACTT GAACCTCCAG TTATCAGTTG CAGACCAGCG TGGAATTGCC 
CAAACCTCAA GTGAGGATGT CAAAGATGCC TCTGTGAAAC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGTCA AGACCTTCTG CATGACTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT TGTGGAAGCA 
TACCTTCTCC AGCACCCAGA TAAGAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTTG ATAGTGAATC CTCAGACATC 
AGTCAGCCAT ACATTGTATG CAGACAGTGT CCTGAATACA GAAGGCAAGC AGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCCACAC AGGCCCTTGG TGGGGAAGCA 
CCTTCAACAT CTGCCAGCTT GACAACAGCA GCCCCGGGTT ATACATGCCC TCTTCAGGGA 
AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCGG ACCGGAGAGC TGAGCATGAG 
CAGGATTCCC GCATCGCCCC TCAACAGTGT GAGTATGTCA GTCTTCCTGG GGTGCCGCCT 
GCCCAGGAGG ACATTCTTGT GTATCCTCCA TGGCTTGGTT TTCTTTTTTT TTTTTTTTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453792.1
CDS	141..1763
Translation	MEPHGEDEQPPPREPWGRLLRLGAEEDEPQILLWKREWTIGRRRGCDLSFPSNKLVSGDHCKLRVDEKSGEVTLEDTSTNGTVINKLRVVKKQTYPLQSGDIIYLVYRKNEPEHNVAYLYESLNGKQSLTQESLEANKENMFHVTKESSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGTISISPKECSSLVANGELSSLSSVFQDNEASFSLLKTEDTEELEPAKKKMKGDGELDLNLQLSVADQRGIAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLLQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATQALGGEAPSTSASLTTAAPGYTCPLQGSHAICTCCFQPMPDRRAEHEQDSRIAPQQCEYVSLPGVPPAQEDILVYPPWLGFLFFFFL

Target ORF information:

RefSeq Version	XM_017598303.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (Chfr), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598303.1

ORF Insert Sequence:

ATGGAGCCAC ACGGGGAAGA CGAGCAGCCG CCACCGCGGG AGCCCTGGGG GAGGCTTCTT 
CGCCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTT CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA GAGTGGATGA AAAATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTAA TCAACAAACT GCGAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGTGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAC GAGCCAGAGC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA ATGGCAAACA GAGCTTAACT CAAGAATCCC TTGAAGCCAA TAAAGAAAAT 
ATGTTCCATG TGACCAAAGA ATCCTCAGGT CCAGGGCAGG GTGATGACCC CCAGGTTCCA 
CTCTTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTTC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCCT CTAGCTCTGG ACCTGGGACT ATAAGCATCT CCCCAAAAGA ATGTAGTTCA 
CTTGTTGCAA ATGGTGAACT CTCTAGCCTT TCTTCAGTTT TCCAAGACAA CGAAGCATCC 
TTTTCTTTGT TGAAAACCGA AGACACTGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AGCTTGACTT GAACCTCCAG TTATCAGTTG CAGACCAGCG TGGAATTGCC 
CAAACCTCAA GTGAGGATGT CAAAGATGCC TCTGTGAAAC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGTCA AGACCTTCTG CATGACTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT TGTGGAAGCA 
TACCTTCTCC AGCACCCAGA TAAGAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTTG ATAGTGAATC CTCAGACATC 
AGTCAGCCAT ACATTGTATG CAGACAGTGT CCTGAATACA GAAGGCAAGC AGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCCACAC AGGCCCTTGG TGGGGAAGCA 
CCTTCAACAT CTGCCAGCTT GACAACAGCA GCCCCGGGTT ATACATGCCC TCTTCAGGGA 
AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCGG ACCGGAGAGC TGAGCATGAG 
CAGGATTCCC GCATCGCCCC TCAACAGTGT GAGTATGTCA GTCTTCCTGG GGTGCCGCCT 
GCCCAGGAGG ACATTCTTGT GTATCCTCCA TGGCTTGGTT TTCTTTTTTT TTTTTTTTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures and Mechanisms of Enzymes Employed in the Synthesis and Degradation of PARP-Dependent Protein ADP-Ribosylation.
Molecular cell58(6)935-46(2015 Jun)
Barkauskaite E,Jankevicius G,Ahel I

Insulin-induced cell division is controlled by the adaptor Grb14 in a Chfr-dependent manner.
Cellular signalling27(4)798-806(2015 Apr)
Perdereau D,Cailliau K,Browaeys-Poly E,Lescuyer A,Carr? N,Benhamed F,Goenaga D,Burnol AF

SRG3/mBAF155 stabilizes the SWI/SNF-like BAF complex by blocking CHFR mediated ubiquitination and degradation of its major components.
Biochemical and biophysical research communications418(3)512-7(2012 Feb)
Jung I,Sohn DH,Choi J,Kim JM,Jeon S,Seol JH,Seong RH

Chfr is linked to tumour metastasis through the downregulation of HDAC1.
Nature cell biology11(3)295-302(2009 Mar)
Oh YM,Kwon YE,Kim JM,Bae SJ,Lee BK,Yoo SJ,Chung CH,Deshaies RJ,Seol JH

Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins.
Nature451(7174)81-5(2008 Jan)
Ahel I,Ahel D,Matsusaka T,Clark AJ,Pines J,Boulton SJ,West SC

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Chfr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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