Ephb3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ephb3
Entrez Gene ID	287989
Full Name	Eph receptor B3
General protein information	Preferred Names Eph receptor B3 Names ephrin type-B receptor 3 EPH-like tyrosine kinase-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105868.1	NP_001099338.1	ephrin type-B receptor 3 precursor
XM_006248561.3	XP_006248623.1	ephrin type-B receptor 3 isoform X1

Pathways from BioSystems

Homologies

	EPHB3	XP_001097522.2
Rattus norvegicus (Norway rat)	Ephb3	NP_001099338.1
Gallus gallus (chicken)	EPHB3	XP_422762.4
Homo sapiens (human)	EPHB3	NP_004434.2
Pan troglodytes (chimpanzee)	EPHB3	XP_003310198.1
Bos taurus (cattle)	EPHB3	NP_001179725.1
Danio rerio (zebrafish)	LOC555957	XP_683712.6
Canis lupus familiaris (dog)	EPHB3	XP_545232.3
Mus musculus (house mouse)	Ephb3	NP_034273.1
Xenopus tropicalis (tropical clawed frog)	LOC100489199	XP_002935194.1

Gene Ontology
Bibliography

Related articles in PubMed

Up-regulated ephrinB3/EphB3 expression in intractable temporal lobe epilepsy patients and pilocarpine induced experimental epilepsy rat model.
Huang H, Li R, Yuan J, Zhou X, Liu X, Ou S, Xu T, Chen Y
Brain research16391-12(2016 05)

Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration.
Lin B, Yin T, Wu YI, Inoue T, Levchenko A
Nature communications66619(2015 Apr)

On the role of Eph signalling in thymus histogenesis; EphB2/B3 and the organizing of the thymic epithelial network.
Garc?a-Ceca J, Jim?nez E, Alfaro D, Cejalvo T, Chumley MJ, Henkemeyer M, Mu?oz JJ, Zapata AG
The International journal of developmental biology53(7)971-82(2009)

Rac-GAP alpha-chimerin regulates motor-circuit formation as a key mediator of EphrinB3/EphA4 forward signaling.
Iwasato T, Katoh H, Nishimaru H, Ishikawa Y, Inoue H, Saito YM, Ando R, Iwama M, Takahashi R, Negishi M, Itohara S
Cell130(4)742-53(2007 Aug)

EphB3 receptor and ligand expression in the adult rat brain.
Willson CA, Foster RD, Onifer SM, Whittemore SR, Miranda JD
Journal of molecular histology37(8-9)369-80(2006 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study found up-regulated expression of ephrinB3/EphB3 in intractable temporal lobe epilepsy patients and experimental temporal lobe epilepsy rats, which suggested that ephrinB3/EphB3 might be involved in the pathogenesis of temporal lobe epilepsy
Title: Up-regulated ephrinB3/EphB3 expression in intractable temporal lobe epilepsy patients and pilocarpine induced experimental epilepsy rat model.

Breast cancer cell motility is regulated in part by Ephb3 signaling.
Title: Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration.

EphB3 may play a role in the maintenance of mature neuronal connections or re-arrangement of synaptic connections during late stages of development.
Title: EphB3 receptor and ligand expression in the adult rat brain.

EphB3 is upregulated after spinal cord injury; this may contribute to an environment in the spinal cord that is inhibitory to axonal regeneration.
Title: Transection of the adult rat spinal cord upregulates EphB3 receptor and ligand expression.

The following Ephb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ephb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa34416	XM_006248561.3 Latest version!	Rattus norvegicus Eph receptor B3 (Ephb3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa41659	NM_001105868.1 Latest version!	Rattus norvegicus Eph receptor B3 (Ephb3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ephb3 ( XM_006248561.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248623.1 Rattus norvegicus Eph receptor B3 (Ephb3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa34416
Clone ID Related Accession (Same CDS sequence)	XM_006248561.3
Accession Version	XM_006248561.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ephrin type-B receptor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248561.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG CCCGCCCGCC GCCGGGGCTT CTGCCGCTGC TCGCTCCGCT GCTGCTGCCG 
CTGCTGCTGC CCGCCGGCTG CCGGGCGCTG GAAGAGACTC TCATGGACAC GAAATGGGTG 
ACGTCCGAGC TGGCATGGAC ATCTCATCCA GAGAGTGGGT GGGAAGAAGT GAGCGGCTAC 
GATGAAGCCA TGAATCCTAT CCGCACGTAT CAGGTGTGTA ACGTGCGCGA GTCCAGCCAG 
AACAACTGGC TGCGGACCGG TTTCATCTGG CGGCGGGAAG TCCAGCGCGT CTACGTGGAG 
CTGAAGTTTA CCGTGAGAGA TTGCAACAGC ATCCCCAACA TCCCTGGCTC CTGCAAGGAA 
ACCTTCAACC TTTTTTACTA CGAGGCTGAT AGCGATGTGG CGTCAGCCTC CTCTCCCTTC 
TGGATGGAGA ACCCCTACGT GAAAGTGGAC ACCATTGCGC CAGATGAGAG CTTCTCGCGG 
CTAGACGCTG GGCGCGTTAA CACCAAAGTG CGCAGCTTCG GGCCGCTTTC CAAAGCCGGC 
TTCTACTTGG CCTTCCAGGA CCAGGGTGCC TGCATGTCAC TCATCTCTGT GCGCGCCTTC 
TACAAGAAGT GTGCATCCAC CACTGCAGGC TTCGCACTCT TCCCCGAGAC CCTCACGGGG 
GCTGAGCCCA CTTCGCTGGT CATTGCCCCT GGCACCTGCA TCGCTAACGC TGTGGAGGTG 
TCTGTACCGC TCAAGCTCTA CTGCAATGGC GACGGGGAGT GGATGGTGCC CGTTGGTGCC 
TGCACCTGCG CTACTGGCCA TGAGCCAGCC GCCAAGGAGA CCCAGTGCCG CGCCTGTCCC 
CCTGGGAGCT ACAAGGCAAA GCAAGGAGAG GGGCCCTGCC TCCCCTGTCC CCCCAATAGC 
CGCACCACCT CGCCGGCTGC CAGCATCTGC ACCTGTCACA ATAATTTCTA CCGCGCAGAC 
TCAGACACAG CGGACAGCGC CTGCACCACG GTGCCGTCTC CCCCCCGGGG TGTGATCTCC 
AATGTGAATG AGACCTCGCT GATCCTCGAG TGGAGTGAGC CCCGGGACCT TGGCGGACGA 
GATGACCTCC TTTATAATGT TATCTGTAAG AAGTGCCGTG GCAGCTCTGG GGCTGGAGGT 
CCGGCGACCT GTTCACGCTG TGATGACAAC GTGGAGTTCG AGCCCCGACA GCTGGGCCTG 
ACCGAGCGCC GGGTCCACAT CAGCCACCTG TTGGCCCACA CCCGCTACAC CTTTGAGGTG 
CAGGCTGTCA ACGGCGTCTC TGGCAAAAGC CCTTTGCCGC CCCGCTATGC AGCTGTGAAT 
ATCACCACCA ACCAGGCCGC CCCATCAGAA GTGCCTACGC TCCACTTGCA CAGCAGTTCA 
GGGAGCAGCC TGACCCTGTC CTGGGCACCC CCGGAGCGGC CTAACGGAGT CATCTTGGAC 
TATGAGATGA AGTACTTTGA GAAGAGTAAA GGCATCGCCT CCACTGTCAC CAGCCAGAAG 
AACTCTGTAC AACTGGACGG ACTGCAGCCC GACGCCCGCT ATGTAGTTCA GGTCCGGGCT 
CGCACAGTAG CAGGTTACGG ACAGTATAGC CGCCCAGCTG AGTTTGAGAC CACGAGTGAA 
AGAGGCTCAG GGGCCCAGCA GCTTCAAGAG CAGCTTCCCC TAATTGTGGG ATCCACCGTA 
GCTGGCTTTG TCTTCATGGT GGTCGTCGTG GTCATTGCTC TTGTCTGCCT CAGGAAGCAG 
CGCCAGGGCC CTGATGCAGA ATACACGGAG AAGTTGCAGC AATACGTTGC CCCCAGGATG 
AAAGTTTACA TTGACCCCTT TACCTACGAG GATCCCAATG AGGCCGTCCG AGAGTTCGCC 
AAGGAGATCG ATGTGTCCTG CGTCAAGATC GAGGAGGTGA TTGGAGCTGG GGAGTTTGGG 
GAAGTGTGCC GGGGTCGGCT GAAACTGCCC GGCCGCCGGG AGGTGTTCGT GGCCATCAAG 
ACACTGAAGG TGGGATACAC GGAGAGGCAG CGGCGGGACT TCCTGAGTGA GGCTTCCATC 
ATGGGTCAAT TTGACCATCC AAATATAATC CGTCTAGAGG GCGTGGTCAC CAAAAGTCGT 
CCAGTCATGA TCCTCACTGA GTTCATGGAG AACTGTGCCC TGGACTCCTT CCTACGGCTC 
AATGACGGGC AGTTCACAGT CATCCAGCTT GTGGGCATGT TGCGTGGCAT TGCTGCCGGC 
ATGAAGTACT TGTCTGAGAT GAACTACGTG CACCGTGACC TCGCTGCCCG CAACATCCTT 
GTCAACAGTA ACTTGGTCTG CAAAGTATCT GACTTTGGGC TCTCCCGCTT CCTGGAGGAC 
GACCCCTCAG ACCCCACCTA CACCAGCTCC CTGGGTGGGA AGATCCCTAT CCGTTGGACC 
GCCCCAGAGG CCATAGCCTA TCGGAAGTTC ACGTCTGCCA GCGATGTCTG GAGCTACGGG 
ATCGTCATGT GGGAGGTCAT GAGCTACGGA GAGCGACCAT ACTGGGACAT GAGCAACCAG 
GATGTCATCA ATGCCGTAGA GCAAGACTAT CGGTTACCAC CCCCCATGGA CTGCCCCACG 
GCGCTGCACC AGCTCATGCT GGACTGTTGG GTGCGGGACC GGAACCTCAG GCCCAAGTTC 
TCCCAAATCG TCAACACGCT AGACAAGCTT ATCCGCAATG CTGCCAGCCT CAAGGTCATC 
GCCAGTGCCC CATCTGGCAT GTCCCAGCCC CTCCTAGACC GCACGGTCCC AGATTATACG 
ACCTTCACGA CGGTGGGCGA CTGGCTAGAT GCCATCAAGA TGGGGAGGTA TAAAGAGAGC 
TTCGTCGGTG CGGGTTTTGC CTCCTTTGAC CTGGTGGCCC AGATGACTGC AGAAGATCTG 
CTAAGGATCG GGGTCACTTT GGCCGGCCAC CAGAAGAAGA TCCTCAGCAG TATCCAGGAC 
ATGCGGCTGC AGATGAACCA GACACTGCCC GTGCAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248623.1
CDS	481..3462
Translation	MAGARPPPGLLPLLAPLLLPLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRREVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIANAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKETQCRACPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDTADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCRGSSGAGGPATCSRCDDNVEFEPRQLGLTERRVHISHLLAHTRYTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLHLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSKGIASTVTSQKNSVQLDGLQPDARYVVQVRARTVAGYGQYSRPAEFETTSERGSGAQQLQEQLPLIVGSTVAGFVFMVVVVVIALVCLRKQRQGPDAEYTEKLQQYVAPRMKVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIGAGEFGEVCRGRLKLPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVIASAPSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVGAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILSSIQDMRLQMNQTLPVQV

Target ORF information:

RefSeq Version	XM_006248561.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Eph receptor B3 (Ephb3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248561.3

ORF Insert Sequence:

ATGGCCGGAG CCCGCCCGCC GCCGGGGCTT CTGCCGCTGC TCGCTCCGCT GCTGCTGCCG 
CTGCTGCTGC CCGCCGGCTG CCGGGCGCTG GAAGAGACTC TCATGGACAC GAAATGGGTG 
ACGTCCGAGC TGGCATGGAC ATCTCATCCA GAGAGTGGGT GGGAAGAAGT GAGCGGCTAC 
GATGAAGCCA TGAATCCTAT CCGCACGTAT CAGGTGTGTA ACGTGCGCGA GTCCAGCCAG 
AACAACTGGC TGCGGACCGG TTTCATCTGG CGGCGGGAAG TCCAGCGCGT CTACGTGGAG 
CTGAAGTTTA CCGTGAGAGA TTGCAACAGC ATCCCCAACA TCCCTGGCTC CTGCAAGGAA 
ACCTTCAACC TTTTTTACTA CGAGGCTGAT AGCGATGTGG CGTCAGCCTC CTCTCCCTTC 
TGGATGGAGA ACCCCTACGT GAAAGTGGAC ACCATTGCGC CAGATGAGAG CTTCTCGCGG 
CTAGACGCTG GGCGCGTTAA CACCAAAGTG CGCAGCTTCG GGCCGCTTTC CAAAGCCGGC 
TTCTACTTGG CCTTCCAGGA CCAGGGTGCC TGCATGTCAC TCATCTCTGT GCGCGCCTTC 
TACAAGAAGT GTGCATCCAC CACTGCAGGC TTCGCACTCT TCCCCGAGAC CCTCACGGGG 
GCTGAGCCCA CTTCGCTGGT CATTGCCCCT GGCACCTGCA TCGCTAACGC TGTGGAGGTG 
TCTGTACCGC TCAAGCTCTA CTGCAATGGC GACGGGGAGT GGATGGTGCC CGTTGGTGCC 
TGCACCTGCG CTACTGGCCA TGAGCCAGCC GCCAAGGAGA CCCAGTGCCG CGCCTGTCCC 
CCTGGGAGCT ACAAGGCAAA GCAAGGAGAG GGGCCCTGCC TCCCCTGTCC CCCCAATAGC 
CGCACCACCT CGCCGGCTGC CAGCATCTGC ACCTGTCACA ATAATTTCTA CCGCGCAGAC 
TCAGACACAG CGGACAGCGC CTGCACCACG GTGCCGTCTC CCCCCCGGGG TGTGATCTCC 
AATGTGAATG AGACCTCGCT GATCCTCGAG TGGAGTGAGC CCCGGGACCT TGGCGGACGA 
GATGACCTCC TTTATAATGT TATCTGTAAG AAGTGCCGTG GCAGCTCTGG GGCTGGAGGT 
CCGGCGACCT GTTCACGCTG TGATGACAAC GTGGAGTTCG AGCCCCGACA GCTGGGCCTG 
ACCGAGCGCC GGGTCCACAT CAGCCACCTG TTGGCCCACA CCCGCTACAC CTTTGAGGTG 
CAGGCTGTCA ACGGCGTCTC TGGCAAAAGC CCTTTGCCGC CCCGCTATGC AGCTGTGAAT 
ATCACCACCA ACCAGGCCGC CCCATCAGAA GTGCCTACGC TCCACTTGCA CAGCAGTTCA 
GGGAGCAGCC TGACCCTGTC CTGGGCACCC CCGGAGCGGC CTAACGGAGT CATCTTGGAC 
TATGAGATGA AGTACTTTGA GAAGAGTAAA GGCATCGCCT CCACTGTCAC CAGCCAGAAG 
AACTCTGTAC AACTGGACGG ACTGCAGCCC GACGCCCGCT ATGTAGTTCA GGTCCGGGCT 
CGCACAGTAG CAGGTTACGG ACAGTATAGC CGCCCAGCTG AGTTTGAGAC CACGAGTGAA 
AGAGGCTCAG GGGCCCAGCA GCTTCAAGAG CAGCTTCCCC TAATTGTGGG ATCCACCGTA 
GCTGGCTTTG TCTTCATGGT GGTCGTCGTG GTCATTGCTC TTGTCTGCCT CAGGAAGCAG 
CGCCAGGGCC CTGATGCAGA ATACACGGAG AAGTTGCAGC AATACGTTGC CCCCAGGATG 
AAAGTTTACA TTGACCCCTT TACCTACGAG GATCCCAATG AGGCCGTCCG AGAGTTCGCC 
AAGGAGATCG ATGTGTCCTG CGTCAAGATC GAGGAGGTGA TTGGAGCTGG GGAGTTTGGG 
GAAGTGTGCC GGGGTCGGCT GAAACTGCCC GGCCGCCGGG AGGTGTTCGT GGCCATCAAG 
ACACTGAAGG TGGGATACAC GGAGAGGCAG CGGCGGGACT TCCTGAGTGA GGCTTCCATC 
ATGGGTCAAT TTGACCATCC AAATATAATC CGTCTAGAGG GCGTGGTCAC CAAAAGTCGT 
CCAGTCATGA TCCTCACTGA GTTCATGGAG AACTGTGCCC TGGACTCCTT CCTACGGCTC 
AATGACGGGC AGTTCACAGT CATCCAGCTT GTGGGCATGT TGCGTGGCAT TGCTGCCGGC 
ATGAAGTACT TGTCTGAGAT GAACTACGTG CACCGTGACC TCGCTGCCCG CAACATCCTT 
GTCAACAGTA ACTTGGTCTG CAAAGTATCT GACTTTGGGC TCTCCCGCTT CCTGGAGGAC 
GACCCCTCAG ACCCCACCTA CACCAGCTCC CTGGGTGGGA AGATCCCTAT CCGTTGGACC 
GCCCCAGAGG CCATAGCCTA TCGGAAGTTC ACGTCTGCCA GCGATGTCTG GAGCTACGGG 
ATCGTCATGT GGGAGGTCAT GAGCTACGGA GAGCGACCAT ACTGGGACAT GAGCAACCAG 
GATGTCATCA ATGCCGTAGA GCAAGACTAT CGGTTACCAC CCCCCATGGA CTGCCCCACG 
GCGCTGCACC AGCTCATGCT GGACTGTTGG GTGCGGGACC GGAACCTCAG GCCCAAGTTC 
TCCCAAATCG TCAACACGCT AGACAAGCTT ATCCGCAATG CTGCCAGCCT CAAGGTCATC 
GCCAGTGCCC CATCTGGCAT GTCCCAGCCC CTCCTAGACC GCACGGTCCC AGATTATACG 
ACCTTCACGA CGGTGGGCGA CTGGCTAGAT GCCATCAAGA TGGGGAGGTA TAAAGAGAGC 
TTCGTCGGTG CGGGTTTTGC CTCCTTTGAC CTGGTGGCCC AGATGACTGC AGAAGATCTG 
CTAAGGATCG GGGTCACTTT GGCCGGCCAC CAGAAGAAGA TCCTCAGCAG TATCCAGGAC 
ATGCGGCTGC AGATGAACCA GACACTGCCC GTGCAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ephb3 ( NM_001105868.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099338.1 Rattus norvegicus Eph receptor B3 (Ephb3), mRNA.

Price & Availability↑

CloneID	ORa41659
Clone ID Related Accession (Same CDS sequence)	NM_001105868.1
Accession Version	NM_001105868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	ephrin type-B receptor 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473999.2. On or before Oct 3, 2007 this sequence version replaced XM_221311.4, XM_001059403.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCCGGAG CCCGCCCGCC GCCGGGGCTT CTGCCGCTGC TCGCTCCGCT GCTGCTGCCG 
CTGCTGCTGC CCGCCGGCTG CCGGGCGCTG GAAGAGACTC TCATGGACAC GAAATGGGTG 
ACGTCCGAGC TGGCATGGAC ATCTCATCCA GAGAGTGGGT GGGAAGAAGT GAGCGGCTAC 
GATGAAGCCA TGAATCCTAT CCGCACGTAT CAGGTGTGTA ACGTGCGCGA GTCCAGCCAG 
AACAACTGGC TGCGGACCGG TTTCATCTGG CGGCGGGAAG TCCAGCGCGT CTACGTGGAG 
CTGAAGTTTA CCGTGAGAGA TTGCAACAGC ATCCCCAACA TCCCTGGCTC CTGCAAGGAA 
ACCTTCAACC TTTTTTACTA CGAGGCTGAT AGCGATGTGG CGTCAGCCTC CTCTCCCTTC 
TGGATGGAGA ACCCCTACGT GAAAGTGGAC ACCATTGCGC CAGATGAGAG CTTCTCGCGG 
CTAGACGCTG GGCGCGTTAA CACCAAAGTG CGCAGCTTCG GGCCGCTTTC CAAAGCCGGC 
TTCTACTTGG CCTTCCAGGA CCAGGGTGCC TGCATGTCAC TCATCTCTGT GCGCGCCTTC 
TACAAGAAGT GTGCATCCAC CACTGCAGGC TTCGCACTCT TCCCCGAGAC CCTCACGGGG 
GCTGAGCCCA CTTCGCTGGT CATTGCCCCT GGCACCTGCA TCGCTAACGC TGTGGAGGTG 
TCTGTACCGC TCAAGCTCTA CTGCAATGGC GACGGGGAGT GGATGGTGCC CGTTGGTGCC 
TGCACCTGCG CTACTGGCCA TGAGCCAGCC GCCAAGGAGA CCCAGTGCCG CGCCTGTCCC 
CCTGGGAGCT ACAAGGCAAA GCAAGGAGAG GGGCCCTGCC TCCCCTGTCC CCCCAATAGC 
CGCACCACCT CGCCGGCTGC CAGCATCTGC ACCTGTCACA ATAATTTCTA CCGCGCAGAC 
TCAGACACAG CGGACAGCGC CTGCACCACG GTGCCGTCTC CCCCCCGGGG TGTGATCTCC 
AATGTGAATG AGACCTCGCT GATCCTCGAG TGGAGTGAGC CCCGGGACCT TGGCGGACGA 
GATGACCTCC TTTATAATGT TATCTGTAAG AAGTGCCGTG GCAGCTCTGG GGCTGGAGGT 
CCGGCGACCT GTTCACGCTG TGATGACAAC GTGGAGTTCG AGCCCCGACA GCTGGGCCTG 
ACCGAGCGCC GGGTCCACAT CAGCCACCTG TTGGCCCACA CCCGCTACAC CTTTGAGGTG 
CAGGCTGTCA ACGGCGTCTC TGGCAAAAGC CCTTTGCCGC CCCGCTATGC AGCTGTGAAT 
ATCACCACCA ACCAGGCCGC CCCATCAGAA GTGCCTACGC TCCACTTGCA CAGCAGTTCA 
GGGAGCAGCC TGACCCTGTC CTGGGCACCC CCGGAGCGGC CTAACGGAGT CATCTTGGAC 
TATGAGATGA AGTACTTTGA GAAGAGTAAA GGCATCGCCT CCACTGTCAC CAGCCAGAAG 
AACTCTGTAC AACTGGACGG ACTGCAGCCC GACGCCCGCT ATGTAGTTCA GGTCCGGGCT 
CGCACAGTAG CAGGTTACGG ACAGTATAGC CGCCCAGCTG AGTTTGAGAC CACGAGTGAA 
AGAGGCTCAG GGGCCCAGCA GCTTCAAGAG CAGCTTCCCC TAATTGTGGG ATCCACCGTA 
GCTGGCTTTG TCTTCATGGT GGTCGTCGTG GTCATTGCTC TTGTCTGCCT CAGGAAGCAG 
CGCCAGGGCC CTGATGCAGA ATACACGGAG AAGTTGCAGC AATACGTTGC CCCCAGGATG 
AAAGTTTACA TTGACCCCTT TACCTACGAG GATCCCAATG AGGCCGTCCG AGAGTTCGCC 
AAGGAGATCG ATGTGTCCTG CGTCAAGATC GAGGAGGTGA TTGGAGCTGG GGAGTTTGGG 
GAAGTGTGCC GGGGTCGGCT GAAACTGCCC GGCCGCCGGG AGGTGTTCGT GGCCATCAAG 
ACACTGAAGG TGGGATACAC GGAGAGGCAG CGGCGGGACT TCCTGAGTGA GGCTTCCATC 
ATGGGTCAAT TTGACCATCC AAATATAATC CGTCTAGAGG GCGTGGTCAC CAAAAGTCGT 
CCAGTCATGA TCCTCACTGA GTTCATGGAG AACTGTGCCC TGGACTCCTT CCTACGGCTC 
AATGACGGGC AGTTCACAGT CATCCAGCTT GTGGGCATGT TGCGTGGCAT TGCTGCCGGC 
ATGAAGTACT TGTCTGAGAT GAACTACGTG CACCGTGACC TCGCTGCCCG CAACATCCTT 
GTCAACAGTA ACTTGGTCTG CAAAGTATCT GACTTTGGGC TCTCCCGCTT CCTGGAGGAC 
GACCCCTCAG ACCCCACCTA CACCAGCTCC CTGGGTGGGA AGATCCCTAT CCGTTGGACC 
GCCCCAGAGG CCATAGCCTA TCGGAAGTTC ACGTCTGCCA GCGATGTCTG GAGCTACGGG 
ATCGTCATGT GGGAGGTCAT GAGCTACGGA GAGCGACCAT ACTGGGACAT GAGCAACCAG 
GATGTCATCA ATGCCGTAGA GCAAGACTAT CGGTTACCAC CCCCCATGGA CTGCCCCACG 
GCGCTGCACC AGCTCATGCT GGACTGTTGG GTGCGGGACC GGAACCTCAG GCCCAAGTTC 
TCCCAAATCG TCAACACGCT AGACAAGCTT ATCCGCAATG CTGCCAGCCT CAAGGTCATC 
GCCAGTGCCC CATCTGGCAT GTCCCAGCCC CTCCTAGACC GCACGGTCCC AGATTATACG 
ACCTTCACGA CGGTGGGCGA CTGGCTAGAT GCCATCAAGA TGGGGAGGTA TAAAGAGAGC 
TTCGTCGGTG CGGGTTTTGC CTCCTTTGAC CTGGTGGCCC AGATGACTGC AGAAGATCTG 
CTAAGGATCG GGGTCACTTT GGCCGGCCAC CAGAAGAAGA TCCTCAGCAG TATCCAGGAC 
ATGCGGCTGC AGATGAACCA GACACTGCCC GTGCAGGAAG TGTGCCCCAA ACCTCTTCAT 
ATTGAAGATG GATTAGAAGA GGGGGTGATA TCCCCTCCCC AGATGCCTCA GGGCCCAGGC 
CTGCCTGCTC TCCAGTCGGG GATCTTCACA ACTCAGATTT GGTTGTGCTT CAGTAGTGGA 
GGTCCTGGTA GGGTCGGGTG GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099338.1
CDS	413..3559
Translation	MAGARPPPGLLPLLAPLLLPLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRREVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIANAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKETQCRACPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDTADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCRGSSGAGGPATCSRCDDNVEFEPRQLGLTERRVHISHLLAHTRYTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLHLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSKGIASTVTSQKNSVQLDGLQPDARYVVQVRARTVAGYGQYSRPAEFETTSERGSGAQQLQEQLPLIVGSTVAGFVFMVVVVVIALVCLRKQRQGPDAEYTEKLQQYVAPRMKVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIGAGEFGEVCRGRLKLPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVIASAPSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVGAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILSSIQDMRLQMNQTLPVQEVCPKPLHIEDGLEEGVISPPQMPQGPGLPALQSGIFTTQIWLCFSSGGPGRVGWG

Target ORF information:

RefSeq Version	NM_001105868.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Eph receptor B3 (Ephb3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105868.1

ORF Insert Sequence:

ATGGCCGGAG CCCGCCCGCC GCCGGGGCTT CTGCCGCTGC TCGCTCCGCT GCTGCTGCCG 
CTGCTGCTGC CCGCCGGCTG CCGGGCGCTG GAAGAGACTC TCATGGACAC GAAATGGGTG 
ACGTCCGAGC TGGCATGGAC ATCTCATCCA GAGAGTGGGT GGGAAGAAGT GAGCGGCTAC 
GATGAAGCCA TGAATCCTAT CCGCACGTAT CAGGTGTGTA ACGTGCGCGA GTCCAGCCAG 
AACAACTGGC TGCGGACCGG TTTCATCTGG CGGCGGGAAG TCCAGCGCGT CTACGTGGAG 
CTGAAGTTTA CCGTGAGAGA TTGCAACAGC ATCCCCAACA TCCCTGGCTC CTGCAAGGAA 
ACCTTCAACC TTTTTTACTA CGAGGCTGAT AGCGATGTGG CGTCAGCCTC CTCTCCCTTC 
TGGATGGAGA ACCCCTACGT GAAAGTGGAC ACCATTGCGC CAGATGAGAG CTTCTCGCGG 
CTAGACGCTG GGCGCGTTAA CACCAAAGTG CGCAGCTTCG GGCCGCTTTC CAAAGCCGGC 
TTCTACTTGG CCTTCCAGGA CCAGGGTGCC TGCATGTCAC TCATCTCTGT GCGCGCCTTC 
TACAAGAAGT GTGCATCCAC CACTGCAGGC TTCGCACTCT TCCCCGAGAC CCTCACGGGG 
GCTGAGCCCA CTTCGCTGGT CATTGCCCCT GGCACCTGCA TCGCTAACGC TGTGGAGGTG 
TCTGTACCGC TCAAGCTCTA CTGCAATGGC GACGGGGAGT GGATGGTGCC CGTTGGTGCC 
TGCACCTGCG CTACTGGCCA TGAGCCAGCC GCCAAGGAGA CCCAGTGCCG CGCCTGTCCC 
CCTGGGAGCT ACAAGGCAAA GCAAGGAGAG GGGCCCTGCC TCCCCTGTCC CCCCAATAGC 
CGCACCACCT CGCCGGCTGC CAGCATCTGC ACCTGTCACA ATAATTTCTA CCGCGCAGAC 
TCAGACACAG CGGACAGCGC CTGCACCACG GTGCCGTCTC CCCCCCGGGG TGTGATCTCC 
AATGTGAATG AGACCTCGCT GATCCTCGAG TGGAGTGAGC CCCGGGACCT TGGCGGACGA 
GATGACCTCC TTTATAATGT TATCTGTAAG AAGTGCCGTG GCAGCTCTGG GGCTGGAGGT 
CCGGCGACCT GTTCACGCTG TGATGACAAC GTGGAGTTCG AGCCCCGACA GCTGGGCCTG 
ACCGAGCGCC GGGTCCACAT CAGCCACCTG TTGGCCCACA CCCGCTACAC CTTTGAGGTG 
CAGGCTGTCA ACGGCGTCTC TGGCAAAAGC CCTTTGCCGC CCCGCTATGC AGCTGTGAAT 
ATCACCACCA ACCAGGCCGC CCCATCAGAA GTGCCTACGC TCCACTTGCA CAGCAGTTCA 
GGGAGCAGCC TGACCCTGTC CTGGGCACCC CCGGAGCGGC CTAACGGAGT CATCTTGGAC 
TATGAGATGA AGTACTTTGA GAAGAGTAAA GGCATCGCCT CCACTGTCAC CAGCCAGAAG 
AACTCTGTAC AACTGGACGG ACTGCAGCCC GACGCCCGCT ATGTAGTTCA GGTCCGGGCT 
CGCACAGTAG CAGGTTACGG ACAGTATAGC CGCCCAGCTG AGTTTGAGAC CACGAGTGAA 
AGAGGCTCAG GGGCCCAGCA GCTTCAAGAG CAGCTTCCCC TAATTGTGGG ATCCACCGTA 
GCTGGCTTTG TCTTCATGGT GGTCGTCGTG GTCATTGCTC TTGTCTGCCT CAGGAAGCAG 
CGCCAGGGCC CTGATGCAGA ATACACGGAG AAGTTGCAGC AATACGTTGC CCCCAGGATG 
AAAGTTTACA TTGACCCCTT TACCTACGAG GATCCCAATG AGGCCGTCCG AGAGTTCGCC 
AAGGAGATCG ATGTGTCCTG CGTCAAGATC GAGGAGGTGA TTGGAGCTGG GGAGTTTGGG 
GAAGTGTGCC GGGGTCGGCT GAAACTGCCC GGCCGCCGGG AGGTGTTCGT GGCCATCAAG 
ACACTGAAGG TGGGATACAC GGAGAGGCAG CGGCGGGACT TCCTGAGTGA GGCTTCCATC 
ATGGGTCAAT TTGACCATCC AAATATAATC CGTCTAGAGG GCGTGGTCAC CAAAAGTCGT 
CCAGTCATGA TCCTCACTGA GTTCATGGAG AACTGTGCCC TGGACTCCTT CCTACGGCTC 
AATGACGGGC AGTTCACAGT CATCCAGCTT GTGGGCATGT TGCGTGGCAT TGCTGCCGGC 
ATGAAGTACT TGTCTGAGAT GAACTACGTG CACCGTGACC TCGCTGCCCG CAACATCCTT 
GTCAACAGTA ACTTGGTCTG CAAAGTATCT GACTTTGGGC TCTCCCGCTT CCTGGAGGAC 
GACCCCTCAG ACCCCACCTA CACCAGCTCC CTGGGTGGGA AGATCCCTAT CCGTTGGACC 
GCCCCAGAGG CCATAGCCTA TCGGAAGTTC ACGTCTGCCA GCGATGTCTG GAGCTACGGG 
ATCGTCATGT GGGAGGTCAT GAGCTACGGA GAGCGACCAT ACTGGGACAT GAGCAACCAG 
GATGTCATCA ATGCCGTAGA GCAAGACTAT CGGTTACCAC CCCCCATGGA CTGCCCCACG 
GCGCTGCACC AGCTCATGCT GGACTGTTGG GTGCGGGACC GGAACCTCAG GCCCAAGTTC 
TCCCAAATCG TCAACACGCT AGACAAGCTT ATCCGCAATG CTGCCAGCCT CAAGGTCATC 
GCCAGTGCCC CATCTGGCAT GTCCCAGCCC CTCCTAGACC GCACGGTCCC AGATTATACG 
ACCTTCACGA CGGTGGGCGA CTGGCTAGAT GCCATCAAGA TGGGGAGGTA TAAAGAGAGC 
TTCGTCGGTG CGGGTTTTGC CTCCTTTGAC CTGGTGGCCC AGATGACTGC AGAAGATCTG 
CTAAGGATCG GGGTCACTTT GGCCGGCCAC CAGAAGAAGA TCCTCAGCAG TATCCAGGAC 
ATGCGGCTGC AGATGAACCA GACACTGCCC GTGCAGGAAG TGTGCCCCAA ACCTCTTCAT 
ATTGAAGATG GATTAGAAGA GGGGGTGATA TCCCCTCCCC AGATGCCTCA GGGCCCAGGC 
CTGCCTGCTC TCCAGTCGGG GATCTTCACA ACTCAGATTT GGTTGTGCTT CAGTAGTGGA 
GGTCCTGGTA GGGTCGGGTG GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Up-regulated ephrinB3/EphB3 expression in intractable temporal lobe epilepsy patients and pilocarpine induced experimental epilepsy rat model.
Brain research16391-12(2016 05)
Huang H,Li R,Yuan J,Zhou X,Liu X,Ou S,Xu T,Chen Y

Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration.
Nature communications66619(2015 Apr)
Lin B,Yin T,Wu YI,Inoue T,Levchenko A

On the role of Eph signalling in thymus histogenesis; EphB2/B3 and the organizing of the thymic epithelial network.
The International journal of developmental biology53(7)971-82(2009)
Garc?a-Ceca J,Jim?nez E,Alfaro D,Cejalvo T,Chumley MJ,Henkemeyer M,Mu?oz JJ,Zapata AG

Rac-GAP alpha-chimerin regulates motor-circuit formation as a key mediator of EphrinB3/EphA4 forward signaling.
Cell130(4)742-53(2007 Aug)
Iwasato T,Katoh H,Nishimaru H,Ishikawa Y,Inoue H,Saito YM,Ando R,Iwama M,Takahashi R,Negishi M,Itohara S

EphB3 receptor and ligand expression in the adult rat brain.
Journal of molecular histology37(8-9)369-80(2006 Nov)
Willson CA,Foster RD,Onifer SM,Whittemore SR,Miranda JD

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Ephb3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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