Ppm1f cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppm1f
Entrez Gene ID	287931
Full Name	protein phosphatase, Mg2+/Mn2+ dependent, 1F
Synonyms	CaMKPase
General protein information	Preferred Names protein phosphatase, Mg2+/Mn2+ dependent, 1F Names protein phosphatase 1F CaM-kinase phosphatase ca(2+)/calmodulin-dependent protein kinase phosphatase calcium/calmodulin-dependent protein kinase phosphatase partner of PIX 2 protein phosphatase 1F (PP2C domain containing)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23
Summary	a calmodulin-dependent protein kinase (CaM-kinase) phosphatase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008768854.2	XP_008767076.1	protein phosphatase 1F isoform X1
XM_006248674.2	XP_006248736.1	protein phosphatase 1F isoform X2
NM_175755.2	NP_786931.1	protein phosphatase 1F
XM_008768855.1	XP_008767077.1	protein phosphatase 1F isoform X1
XM_008768853.2	XP_008767075.1	protein phosphatase 1F isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ppm1f	NP_001018165.1
Homo sapiens (human)	PPM1F	NP_055449.1
Canis lupus familiaris (dog)	PPM1F	XP_543574.3
Rattus norvegicus (Norway rat)	Ppm1f	NP_786931.1
Oryza sativa (rice)	Os06g0526700	NP_001057765.1
Xenopus tropicalis (tropical clawed frog)	ppm1f	XP_002931930.1
Mus musculus (house mouse)	Ppm1f	NP_789803.1
Oryza sativa (rice)	Os06g0526800	NP_001057766.1
Pan troglodytes (chimpanzee)	PPM1F	XP_003317173.1
Bos taurus (cattle)	PPM1F	XP_002694747.3
Drosophila melanogaster (fruit fly)	CG10376	NP_609899.1
	PPM1F	XP_001089477.1
Gallus gallus (chicken)	PPM1F	XP_415067.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002147	XP_308048.5

Gene Ontology
Bibliography

Related articles in PubMed

miR-149 represses metastasis of hepatocellular carcinoma by targeting actin-regulatory proteins PPM1F.
Luo G, Chao YL, Tang B, Li BS, Xiao YF, Xie R, Wang SM, Wu YY, Dong H, Liu XD, Yang SM
Oncotarget6(35)37808-23(2015 Nov)

POPX2 phosphatase regulates the KIF3 kinesin motor complex.
Phang HQ, Hoon JL, Lai SK, Zeng Y, Chiam KH, Li HY, Koh CG
Journal of cell science127(Pt 4)727-39(2014 Feb)

An active C-terminally truncated form of Ca (2+) /calmodulin-dependent protein kinase phosphatase-N (CaMKP-N/PPM1E).
Ishida A, Tsumura K, Oue M, Takenaka Y, Shigeri Y, Goshima N, Ishihara Y, Hirano T, Baba H, Sueyoshi N, Kameshita I, Yamazaki T
BioMed research international2013134813(2013)

Ppm1E is an in cellulo AMP-activated protein kinase phosphatase.
Voss M, Paterson J, Kelsall IR, Mart?n-Granados C, Hastie CJ, Peggie MW, Cohen PT
Cellular signalling23(1)114-24(2011 Jan)

The POPX2 phosphatase regulates cancer cell motility and invasiveness.
Susila A, Chan H, Loh AX, Phang HQ, Wong ET, Tergaonkar V, Koh CG
Cell cycle (Georgetown, Tex.)9(1)179-87(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The truncated CaMKP-N may play an important physiological role in neuronal cells.
Title: An active C-terminally truncated form of Ca (2+) /calmodulin-dependent protein kinase phosphatase-N (CaMKP-N/PPM1E).

The following Ppm1f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppm1f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03923	NM_175755.2 Latest version!	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa03923	XM_006248674.2 Latest version!	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa44651	XM_008768854.2 Latest version!	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa44651	XM_008768855.1 Latest version!	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa44651	XM_008768853.2 Latest version!	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppm1f ( NM_175755.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_786931.1 Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), mRNA.

Price & Availability↑

CloneID	ORa03923
Clone ID Related Accession (Same CDS sequence)	XM_006248674.2 , NM_175755.2
Accession Version	NM_175755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB023634.1. On Jun 3, 2003 this sequence version replaced NM_175755.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB023634.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCCTCTG GAGCCCCACA GAACAGCAGC CAGATGGCCT GTGACGGCGA GATCCCAGGT 
TTCCTGGACA CACTCCTCCA AGACTTCCCA GCCCCACTGA GCCTGGAGAG CCCTTTGCCA 
TGGAAGGTCC CAGGGACAGT CCTGGGCCAG GAGGAGGTGG AGGCTGAGCT GACGGAGCTG 
GCAATGGGTT TCCTGGGCAG CAGGAATGCA CCCCCAGCAG TTGCTGCGGC TGTGACCCAC 
GAGGCAATTT CCCAGCTGCT TCAGACAGAC CTCTCTGAAT TTAAGAGGTT GCCTGAACAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGAGA GTCCTCACGA CCCTACTGGA TGCCAAAGGC 
CTGTCCCGGA GCTTCTTTAA CTGTCTTTGG GAAGTATGCA GCCAGTGGCA GAAACGGGTG 
CCACTGACTG CCCAGGCCCC TCAGCGCAAG TGGCTGGTCT CTATCCATGC CATCCGGAAC 
ACACGACGAA AGATGGAGGA TCGACACGTG TCCCTTCCTG CTTTCAACCA CCTCTTCGGC 
TTGTCTGACT CCGTGCACCG CGCCTACTTC GCTGTGTTTG ATGGTCATGG AGGTGTGGAT 
GCAGCACGGT ATGCCTCTGT CCACGTGCAC ACCAACGCCT CTCACCAGCC AGAGCTGCTC 
ACAGACCCTG CAGCAGCTCT CAAAGAAGCC TTCCGGCATA CTGACCAGAT GTTTCTCCAG 
AAAGCCAAGC GAGAGCGACT GCAGAGCGGT ACCACAGGTG TGTGTGCGCT CATCACAGGA 
GCAGCCCTGC ATGTTGCCTG GCTTGGAGAC TCCCAGGTCA TCCTGGTACA GCAAGGACAG 
GTAGTGAAGC TGATGGAGCC ACACAAACCG GAACGACAGG ATGAGAAATC ACGCATTGAA 
GCGCTGGGCG GCTTTGTTTC TCTCATGGAC TGCTGGAGAG TCAACGGGAC CCTGGCAGTG 
TCCAGAGCCA TTGGGGATGT CTTCCAGAAG CCTTATGTGT CTGGCGAGGC AGATGCAGCA 
TCCAGAGAGC TGACAGGCTT GGAGGACTAC CTGCTGCTTG CCTGTGACGG TTTCTTCGAT 
GTCGTCCCCC ACCATGAAAT CCCGGGTCTT GTCCATGGCC ACTTGCTCAG GCAGAAGGGC 
AGTGGGATGC ACGTTGCTGA GGAGCTGGTG GCTGTAGCCC GTGACCGGGG TTCCCATGAC 
AACATTACAG TCATGGTGGT TTTCCTTAGG GACCCCCTGG AGCTGCTGGA GGGTGGGGGG 
CAGGGGGCAG GGGGTGCCCA GGCAGATGTA GGAAGTCAGG ACTTGTCCAC TGGCCTCTCA 
GAACTTGAGA TCAATACCTC ACAGAGAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_786931.1
CDS	94..1446
Translation	MASGAPQNSSQMACDGEIPGFLDTLLQDFPAPLSLESPLPWKVPGTVLGQEEVEAELTELAMGFLGSRNAPPAVAAAVTHEAISQLLQTDLSEFKRLPEQEEEEEEEEERVLTTLLDAKGLSRSFFNCLWEVCSQWQKRVPLTAQAPQRKWLVSIHAIRNTRRKMEDRHVSLPAFNHLFGLSDSVHRAYFAVFDGHGGVDAARYASVHVHTNASHQPELLTDPAAALKEAFRHTDQMFLQKAKRERLQSGTTGVCALITGAALHVAWLGDSQVILVQQGQVVKLMEPHKPERQDEKSRIEALGGFVSLMDCWRVNGTLAVSRAIGDVFQKPYVSGEADAASRELTGLEDYLLLACDGFFDVVPHHEIPGLVHGHLLRQKGSGMHVAEELVAVARDRGSHDNITVMVVFLRDPLELLEGGGQGAGGAQADVGSQDLSTGLSELEINTSQRS

Target ORF information:

RefSeq Version	NM_175755.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175755.2

ORF Insert Sequence:

ATGGCCTCTG GAGCCCCACA GAACAGCAGC CAGATGGCCT GTGACGGCGA GATCCCAGGT 
TTCCTGGACA CACTCCTCCA AGACTTCCCA GCCCCACTGA GCCTGGAGAG CCCTTTGCCA 
TGGAAGGTCC CAGGGACAGT CCTGGGCCAG GAGGAGGTGG AGGCTGAGCT GACGGAGCTG 
GCAATGGGTT TCCTGGGCAG CAGGAATGCA CCCCCAGCAG TTGCTGCGGC TGTGACCCAC 
GAGGCAATTT CCCAGCTGCT TCAGACAGAC CTCTCTGAAT TTAAGAGGTT GCCTGAACAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGAGA GTCCTCACGA CCCTACTGGA TGCCAAAGGC 
CTGTCCCGGA GCTTCTTTAA CTGTCTTTGG GAAGTATGCA GCCAGTGGCA GAAACGGGTG 
CCACTGACTG CCCAGGCCCC TCAGCGCAAG TGGCTGGTCT CTATCCATGC CATCCGGAAC 
ACACGACGAA AGATGGAGGA TCGACACGTG TCCCTTCCTG CTTTCAACCA CCTCTTCGGC 
TTGTCTGACT CCGTGCACCG CGCCTACTTC GCTGTGTTTG ATGGTCATGG AGGTGTGGAT 
GCAGCACGGT ATGCCTCTGT CCACGTGCAC ACCAACGCCT CTCACCAGCC AGAGCTGCTC 
ACAGACCCTG CAGCAGCTCT CAAAGAAGCC TTCCGGCATA CTGACCAGAT GTTTCTCCAG 
AAAGCCAAGC GAGAGCGACT GCAGAGCGGT ACCACAGGTG TGTGTGCGCT CATCACAGGA 
GCAGCCCTGC ATGTTGCCTG GCTTGGAGAC TCCCAGGTCA TCCTGGTACA GCAAGGACAG 
GTAGTGAAGC TGATGGAGCC ACACAAACCG GAACGACAGG ATGAGAAATC ACGCATTGAA 
GCGCTGGGCG GCTTTGTTTC TCTCATGGAC TGCTGGAGAG TCAACGGGAC CCTGGCAGTG 
TCCAGAGCCA TTGGGGATGT CTTCCAGAAG CCTTATGTGT CTGGCGAGGC AGATGCAGCA 
TCCAGAGAGC TGACAGGCTT GGAGGACTAC CTGCTGCTTG CCTGTGACGG TTTCTTCGAT 
GTCGTCCCCC ACCATGAAAT CCCGGGTCTT GTCCATGGCC ACTTGCTCAG GCAGAAGGGC 
AGTGGGATGC ACGTTGCTGA GGAGCTGGTG GCTGTAGCCC GTGACCGGGG TTCCCATGAC 
AACATTACAG TCATGGTGGT TTTCCTTAGG GACCCCCTGG AGCTGCTGGA GGGTGGGGGG 
CAGGGGGCAG GGGGTGCCCA GGCAGATGTA GGAAGTCAGG ACTTGTCCAC TGGCCTCTCA 
GAACTTGAGA TCAATACCTC ACAGAGAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppm1f ( XM_006248674.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248736.1 Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa03923
Clone ID Related Accession (Same CDS sequence)	XM_006248674.2 , NM_175755.2
Accession Version	XM_006248674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTG GAGCCCCACA GAACAGCAGC CAGATGGCCT GTGACGGCGA GATCCCAGGT 
TTCCTGGACA CACTCCTCCA AGACTTCCCA GCCCCACTGA GCCTGGAGAG CCCTTTGCCA 
TGGAAGGTCC CAGGGACAGT CCTGGGCCAG GAGGAGGTGG AGGCTGAGCT GACGGAGCTG 
GCAATGGGTT TCCTGGGCAG CAGGAATGCA CCCCCAGCAG TTGCTGCGGC TGTGACCCAC 
GAGGCAATTT CCCAGCTGCT TCAGACAGAC CTCTCTGAAT TTAAGAGGTT GCCTGAACAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGAGA GTCCTCACGA CCCTACTGGA TGCCAAAGGC 
CTGTCCCGGA GCTTCTTTAA CTGTCTTTGG GAAGTATGCA GCCAGTGGCA GAAACGGGTG 
CCACTGACTG CCCAGGCCCC TCAGCGCAAG TGGCTGGTCT CTATCCATGC CATCCGGAAC 
ACACGACGAA AGATGGAGGA TCGACACGTG TCCCTTCCTG CTTTCAACCA CCTCTTCGGC 
TTGTCTGACT CCGTGCACCG CGCCTACTTC GCTGTGTTTG ATGGTCATGG AGGTGTGGAT 
GCAGCACGGT ATGCCTCTGT CCACGTGCAC ACCAACGCCT CTCACCAGCC AGAGCTGCTC 
ACAGACCCTG CAGCAGCTCT CAAAGAAGCC TTCCGGCATA CTGACCAGAT GTTTCTCCAG 
AAAGCCAAGC GAGAGCGACT GCAGAGCGGT ACCACAGGTG TGTGTGCGCT CATCACAGGA 
GCAGCCCTGC ATGTTGCCTG GCTTGGAGAC TCCCAGGTCA TCCTGGTACA GCAAGGACAG 
GTAGTGAAGC TGATGGAGCC ACACAAACCG GAACGACAGG ATGAGAAATC ACGCATTGAA 
GCGCTGGGCG GCTTTGTTTC TCTCATGGAC TGCTGGAGAG TCAACGGGAC CCTGGCAGTG 
TCCAGAGCCA TTGGGGATGT CTTCCAGAAG CCTTATGTGT CTGGCGAGGC AGATGCAGCA 
TCCAGAGAGC TGACAGGCTT GGAGGACTAC CTGCTGCTTG CCTGTGACGG TTTCTTCGAT 
GTCGTCCCCC ACCATGAAAT CCCGGGTCTT GTCCATGGCC ACTTGCTCAG GCAGAAGGGC 
AGTGGGATGC ACGTTGCTGA GGAGCTGGTG GCTGTAGCCC GTGACCGGGG TTCCCATGAC 
AACATTACAG TCATGGTGGT TTTCCTTAGG GACCCCCTGG AGCTGCTGGA GGGTGGGGGG 
CAGGGGGCAG GGGGTGCCCA GGCAGATGTA GGAAGTCAGG ACTTGTCCAC TGGCCTCTCA 
GAACTTGAGA TCAATACCTC ACAGAGAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248736.1
CDS	199..1551
Translation	MASGAPQNSSQMACDGEIPGFLDTLLQDFPAPLSLESPLPWKVPGTVLGQEEVEAELTELAMGFLGSRNAPPAVAAAVTHEAISQLLQTDLSEFKRLPEQEEEEEEEEERVLTTLLDAKGLSRSFFNCLWEVCSQWQKRVPLTAQAPQRKWLVSIHAIRNTRRKMEDRHVSLPAFNHLFGLSDSVHRAYFAVFDGHGGVDAARYASVHVHTNASHQPELLTDPAAALKEAFRHTDQMFLQKAKRERLQSGTTGVCALITGAALHVAWLGDSQVILVQQGQVVKLMEPHKPERQDEKSRIEALGGFVSLMDCWRVNGTLAVSRAIGDVFQKPYVSGEADAASRELTGLEDYLLLACDGFFDVVPHHEIPGLVHGHLLRQKGSGMHVAEELVAVARDRGSHDNITVMVVFLRDPLELLEGGGQGAGGAQADVGSQDLSTGLSELEINTSQRS

Target ORF information:

RefSeq Version	XM_006248674.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248674.2

ORF Insert Sequence:

ATGGCCTCTG GAGCCCCACA GAACAGCAGC CAGATGGCCT GTGACGGCGA GATCCCAGGT 
TTCCTGGACA CACTCCTCCA AGACTTCCCA GCCCCACTGA GCCTGGAGAG CCCTTTGCCA 
TGGAAGGTCC CAGGGACAGT CCTGGGCCAG GAGGAGGTGG AGGCTGAGCT GACGGAGCTG 
GCAATGGGTT TCCTGGGCAG CAGGAATGCA CCCCCAGCAG TTGCTGCGGC TGTGACCCAC 
GAGGCAATTT CCCAGCTGCT TCAGACAGAC CTCTCTGAAT TTAAGAGGTT GCCTGAACAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGAGA GTCCTCACGA CCCTACTGGA TGCCAAAGGC 
CTGTCCCGGA GCTTCTTTAA CTGTCTTTGG GAAGTATGCA GCCAGTGGCA GAAACGGGTG 
CCACTGACTG CCCAGGCCCC TCAGCGCAAG TGGCTGGTCT CTATCCATGC CATCCGGAAC 
ACACGACGAA AGATGGAGGA TCGACACGTG TCCCTTCCTG CTTTCAACCA CCTCTTCGGC 
TTGTCTGACT CCGTGCACCG CGCCTACTTC GCTGTGTTTG ATGGTCATGG AGGTGTGGAT 
GCAGCACGGT ATGCCTCTGT CCACGTGCAC ACCAACGCCT CTCACCAGCC AGAGCTGCTC 
ACAGACCCTG CAGCAGCTCT CAAAGAAGCC TTCCGGCATA CTGACCAGAT GTTTCTCCAG 
AAAGCCAAGC GAGAGCGACT GCAGAGCGGT ACCACAGGTG TGTGTGCGCT CATCACAGGA 
GCAGCCCTGC ATGTTGCCTG GCTTGGAGAC TCCCAGGTCA TCCTGGTACA GCAAGGACAG 
GTAGTGAAGC TGATGGAGCC ACACAAACCG GAACGACAGG ATGAGAAATC ACGCATTGAA 
GCGCTGGGCG GCTTTGTTTC TCTCATGGAC TGCTGGAGAG TCAACGGGAC CCTGGCAGTG 
TCCAGAGCCA TTGGGGATGT CTTCCAGAAG CCTTATGTGT CTGGCGAGGC AGATGCAGCA 
TCCAGAGAGC TGACAGGCTT GGAGGACTAC CTGCTGCTTG CCTGTGACGG TTTCTTCGAT 
GTCGTCCCCC ACCATGAAAT CCCGGGTCTT GTCCATGGCC ACTTGCTCAG GCAGAAGGGC 
AGTGGGATGC ACGTTGCTGA GGAGCTGGTG GCTGTAGCCC GTGACCGGGG TTCCCATGAC 
AACATTACAG TCATGGTGGT TTTCCTTAGG GACCCCCTGG AGCTGCTGGA GGGTGGGGGG 
CAGGGGGCAG GGGGTGCCCA GGCAGATGTA GGAAGTCAGG ACTTGTCCAC TGGCCTCTCA 
GAACTTGAGA TCAATACCTC ACAGAGAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppm1f ( XM_008768854.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767076.1 Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa44651
Clone ID Related Accession (Same CDS sequence)	XM_008768854.2 , XM_008768855.1 , XM_008768853.2
Accession Version	XM_008768854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTG GAGCCCCACA GAACAGCAGC CAGATGGCCT GTGACGGCGA GATCCCAGGT 
TTCCTGGACA CACTCCTCCA AGACTTCCCA GCCCCACTGA GCCTGGAGAG CCCTTTGCCA 
TGGAAGGTCC CAGGGACAGT CCTGGGCCAG GAGGAGGTGG AGGCTGAGCT GACGGAGCTG 
GCAATGGGTT TCCTGGGCAG CAGGAATGCA CCCCCAGCAG TTGCTGCGGC TGTGACCCAC 
GAGGCAATTT CCCAGCTGCT TCAGACAGAC CTCTCTGAAT TTAAGAGGTT GCCTGAACAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGAGA GTCCTCACGA CCCTACTGGA TGCCAAAGGC 
CTGTCCCGGA GCTTCTTTAA CTGTCTTTGG GAAGTATGCA GCCAGTGGCA GAAACGGGTG 
CCACTGACTG CCCAGGCCCC TCAGCGCAAG TGGCTGGTCT CTATCCATGC CATCCGGAAC 
ACACGACGAA AGATGGAGGA TCGACACGTG TCCCTTCCTG CTTTCAACCA CCTCTTCGGC 
TTGTCTGACT CCGTGCACCG CGCCTACTTC GCTGTGTTTG ATGGTCATGG AGGTGTGGAT 
GCAGCACGGT ATGCCTCTGT CCACGTGCAC ACCAACGCCT CTCACCAGCC AGAGCTGCTC 
ACAGACCCTG CAGCAGCTCT CAAAGAAGCC TTCCGGCATA CTGACCAGAT GTTTCTCCAG 
AAAGCCAAGC GAGAGCGACT GCAGAGCGGT ACCACAGGTG TGTGTGCGCT CATCACAGGA 
GCAGCCCTGC ATGTTGCCTG GCTTGGAGAC TCCCAGGTCA TCCTGGTACA GCAAGGACAG 
GTAGTGAAGC TGATGGAGCC ACACAAACCG GAACGACAGG ATGAGAAATC ACGCATTGAA 
GCGCTGGGCG GCTTTGTTTC TCTCATGGAC TGCTGGAGAG TCAACGGGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGGGATGT CTTCCAGAAG CCTTATGTGT CTGGCGAGGC AGATGCAGCA 
TCCAGAGAGC TGACAGGCTT GGAGGACTAC CTGCTGCTTG CCTGTGACGG TTTCTTCGAT 
GTCGTCCCCC ACCATGAAAT CCCGGGTCTT GTCCATGGCC ACTTGCTCAG GCAGAAGGGC 
AGTGGGATGC ACGTTGCTGA GGAGCTGGTG GCTGTAGCCC GTGACCGGGG TTCCCATGAC 
AACATTACAG TCATGGTGGT TTTCCTTAGG GACCCCCTGG AGCTGCTGGA GGGTGGGGGG 
CAGGGGGCAG GGGGTGCCCA GGCAGATGTA GGAAGTCAGG ACTTGTCCAC TGGCCTCTCA 
GAACTTGAGA TCAATACCTC ACAGAGAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767076.1
CDS	177..1709
Translation	MASGAPQNSSQMACDGEIPGFLDTLLQDFPAPLSLESPLPWKVPGTVLGQEEVEAELTELAMGFLGSRNAPPAVAAAVTHEAISQLLQTDLSEFKRLPEQEEEEEEEEERVLTTLLDAKGLSRSFFNCLWEVCSQWQKRVPLTAQAPQRKWLVSIHAIRNTRRKMEDRHVSLPAFNHLFGLSDSVHRAYFAVFDGHGGVDAARYASVHVHTNASHQPELLTDPAAALKEAFRHTDQMFLQKAKRERLQSGTTGVCALITGAALHVAWLGDSQVILVQQGQVVKLMEPHKPERQDEKSRIEALGGFVSLMDCWRVNGTLAVSRAIGKTLAVSRAIGKTLAVSRAIGKTLAVSRAIGKTLAVSRAIGKTLAVSRAIGKTLAVSRAIGDVFQKPYVSGEADAASRELTGLEDYLLLACDGFFDVVPHHEIPGLVHGHLLRQKGSGMHVAEELVAVARDRGSHDNITVMVVFLRDPLELLEGGGQGAGGAQADVGSQDLSTGLSELEINTSQRS

Target ORF information:

RefSeq Version	XM_008768854.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768854.2

ORF Insert Sequence:

ATGGCCTCTG GAGCCCCACA GAACAGCAGC CAGATGGCCT GTGACGGCGA GATCCCAGGT 
TTCCTGGACA CACTCCTCCA AGACTTCCCA GCCCCACTGA GCCTGGAGAG CCCTTTGCCA 
TGGAAGGTCC CAGGGACAGT CCTGGGCCAG GAGGAGGTGG AGGCTGAGCT GACGGAGCTG 
GCAATGGGTT TCCTGGGCAG CAGGAATGCA CCCCCAGCAG TTGCTGCGGC TGTGACCCAC 
GAGGCAATTT CCCAGCTGCT TCAGACAGAC CTCTCTGAAT TTAAGAGGTT GCCTGAACAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGAGA GTCCTCACGA CCCTACTGGA TGCCAAAGGC 
CTGTCCCGGA GCTTCTTTAA CTGTCTTTGG GAAGTATGCA GCCAGTGGCA GAAACGGGTG 
CCACTGACTG CCCAGGCCCC TCAGCGCAAG TGGCTGGTCT CTATCCATGC CATCCGGAAC 
ACACGACGAA AGATGGAGGA TCGACACGTG TCCCTTCCTG CTTTCAACCA CCTCTTCGGC 
TTGTCTGACT CCGTGCACCG CGCCTACTTC GCTGTGTTTG ATGGTCATGG AGGTGTGGAT 
GCAGCACGGT ATGCCTCTGT CCACGTGCAC ACCAACGCCT CTCACCAGCC AGAGCTGCTC 
ACAGACCCTG CAGCAGCTCT CAAAGAAGCC TTCCGGCATA CTGACCAGAT GTTTCTCCAG 
AAAGCCAAGC GAGAGCGACT GCAGAGCGGT ACCACAGGTG TGTGTGCGCT CATCACAGGA 
GCAGCCCTGC ATGTTGCCTG GCTTGGAGAC TCCCAGGTCA TCCTGGTACA GCAAGGACAG 
GTAGTGAAGC TGATGGAGCC ACACAAACCG GAACGACAGG ATGAGAAATC ACGCATTGAA 
GCGCTGGGCG GCTTTGTTTC TCTCATGGAC TGCTGGAGAG TCAACGGGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGGGATGT CTTCCAGAAG CCTTATGTGT CTGGCGAGGC AGATGCAGCA 
TCCAGAGAGC TGACAGGCTT GGAGGACTAC CTGCTGCTTG CCTGTGACGG TTTCTTCGAT 
GTCGTCCCCC ACCATGAAAT CCCGGGTCTT GTCCATGGCC ACTTGCTCAG GCAGAAGGGC 
AGTGGGATGC ACGTTGCTGA GGAGCTGGTG GCTGTAGCCC GTGACCGGGG TTCCCATGAC 
AACATTACAG TCATGGTGGT TTTCCTTAGG GACCCCCTGG AGCTGCTGGA GGGTGGGGGG 
CAGGGGGCAG GGGGTGCCCA GGCAGATGTA GGAAGTCAGG ACTTGTCCAC TGGCCTCTCA 
GAACTTGAGA TCAATACCTC ACAGAGAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppm1f ( XM_008768855.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767077.1 Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), transcript variant X3, mRNA.

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CloneID	ORa44651
Clone ID Related Accession (Same CDS sequence)	XM_008768854.2 , XM_008768855.1 , XM_008768853.2
Accession Version	XM_008768855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTG GAGCCCCACA GAACAGCAGC CAGATGGCCT GTGACGGCGA GATCCCAGGT 
TTCCTGGACA CACTCCTCCA AGACTTCCCA GCCCCACTGA GCCTGGAGAG CCCTTTGCCA 
TGGAAGGTCC CAGGGACAGT CCTGGGCCAG GAGGAGGTGG AGGCTGAGCT GACGGAGCTG 
GCAATGGGTT TCCTGGGCAG CAGGAATGCA CCCCCAGCAG TTGCTGCGGC TGTGACCCAC 
GAGGCAATTT CCCAGCTGCT TCAGACAGAC CTCTCTGAAT TTAAGAGGTT GCCTGAACAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGAGA GTCCTCACGA CCCTACTGGA TGCCAAAGGC 
CTGTCCCGGA GCTTCTTTAA CTGTCTTTGG GAAGTATGCA GCCAGTGGCA GAAACGGGTG 
CCACTGACTG CCCAGGCCCC TCAGCGCAAG TGGCTGGTCT CTATCCATGC CATCCGGAAC 
ACACGACGAA AGATGGAGGA TCGACACGTG TCCCTTCCTG CTTTCAACCA CCTCTTCGGC 
TTGTCTGACT CCGTGCACCG CGCCTACTTC GCTGTGTTTG ATGGTCATGG AGGTGTGGAT 
GCAGCACGGT ATGCCTCTGT CCACGTGCAC ACCAACGCCT CTCACCAGCC AGAGCTGCTC 
ACAGACCCTG CAGCAGCTCT CAAAGAAGCC TTCCGGCATA CTGACCAGAT GTTTCTCCAG 
AAAGCCAAGC GAGAGCGACT GCAGAGCGGT ACCACAGGTG TGTGTGCGCT CATCACAGGA 
GCAGCCCTGC ATGTTGCCTG GCTTGGAGAC TCCCAGGTCA TCCTGGTACA GCAAGGACAG 
GTAGTGAAGC TGATGGAGCC ACACAAACCG GAACGACAGG ATGAGAAATC ACGCATTGAA 
GCGCTGGGCG GCTTTGTTTC TCTCATGGAC TGCTGGAGAG TCAACGGGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGGGATGT CTTCCAGAAG CCTTATGTGT CTGGCGAGGC AGATGCAGCA 
TCCAGAGAGC TGACAGGCTT GGAGGACTAC CTGCTGCTTG CCTGTGACGG TTTCTTCGAT 
GTCGTCCCCC ACCATGAAAT CCCGGGTCTT GTCCATGGCC ACTTGCTCAG GCAGAAGGGC 
AGTGGGATGC ACGTTGCTGA GGAGCTGGTG GCTGTAGCCC GTGACCGGGG TTCCCATGAC 
AACATTACAG TCATGGTGGT TTTCCTTAGG GACCCCCTGG AGCTGCTGGA GGGTGGGGGG 
CAGGGGGCAG GGGGTGCCCA GGCAGATGTA GGAAGTCAGG ACTTGTCCAC TGGCCTCTCA 
GAACTTGAGA TCAATACCTC ACAGAGAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767077.1
CDS	199..1731
Translation	MASGAPQNSSQMACDGEIPGFLDTLLQDFPAPLSLESPLPWKVPGTVLGQEEVEAELTELAMGFLGSRNAPPAVAAAVTHEAISQLLQTDLSEFKRLPEQEEEEEEEEERVLTTLLDAKGLSRSFFNCLWEVCSQWQKRVPLTAQAPQRKWLVSIHAIRNTRRKMEDRHVSLPAFNHLFGLSDSVHRAYFAVFDGHGGVDAARYASVHVHTNASHQPELLTDPAAALKEAFRHTDQMFLQKAKRERLQSGTTGVCALITGAALHVAWLGDSQVILVQQGQVVKLMEPHKPERQDEKSRIEALGGFVSLMDCWRVNGTLAVSRAIGKTLAVSRAIGKTLAVSRAIGKTLAVSRAIGKTLAVSRAIGKTLAVSRAIGKTLAVSRAIGDVFQKPYVSGEADAASRELTGLEDYLLLACDGFFDVVPHHEIPGLVHGHLLRQKGSGMHVAEELVAVARDRGSHDNITVMVVFLRDPLELLEGGGQGAGGAQADVGSQDLSTGLSELEINTSQRS

Target ORF information:

RefSeq Version	XM_008768855.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768855.1

ORF Insert Sequence:

ATGGCCTCTG GAGCCCCACA GAACAGCAGC CAGATGGCCT GTGACGGCGA GATCCCAGGT 
TTCCTGGACA CACTCCTCCA AGACTTCCCA GCCCCACTGA GCCTGGAGAG CCCTTTGCCA 
TGGAAGGTCC CAGGGACAGT CCTGGGCCAG GAGGAGGTGG AGGCTGAGCT GACGGAGCTG 
GCAATGGGTT TCCTGGGCAG CAGGAATGCA CCCCCAGCAG TTGCTGCGGC TGTGACCCAC 
GAGGCAATTT CCCAGCTGCT TCAGACAGAC CTCTCTGAAT TTAAGAGGTT GCCTGAACAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGAGA GTCCTCACGA CCCTACTGGA TGCCAAAGGC 
CTGTCCCGGA GCTTCTTTAA CTGTCTTTGG GAAGTATGCA GCCAGTGGCA GAAACGGGTG 
CCACTGACTG CCCAGGCCCC TCAGCGCAAG TGGCTGGTCT CTATCCATGC CATCCGGAAC 
ACACGACGAA AGATGGAGGA TCGACACGTG TCCCTTCCTG CTTTCAACCA CCTCTTCGGC 
TTGTCTGACT CCGTGCACCG CGCCTACTTC GCTGTGTTTG ATGGTCATGG AGGTGTGGAT 
GCAGCACGGT ATGCCTCTGT CCACGTGCAC ACCAACGCCT CTCACCAGCC AGAGCTGCTC 
ACAGACCCTG CAGCAGCTCT CAAAGAAGCC TTCCGGCATA CTGACCAGAT GTTTCTCCAG 
AAAGCCAAGC GAGAGCGACT GCAGAGCGGT ACCACAGGTG TGTGTGCGCT CATCACAGGA 
GCAGCCCTGC ATGTTGCCTG GCTTGGAGAC TCCCAGGTCA TCCTGGTACA GCAAGGACAG 
GTAGTGAAGC TGATGGAGCC ACACAAACCG GAACGACAGG ATGAGAAATC ACGCATTGAA 
GCGCTGGGCG GCTTTGTTTC TCTCATGGAC TGCTGGAGAG TCAACGGGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGGGATGT CTTCCAGAAG CCTTATGTGT CTGGCGAGGC AGATGCAGCA 
TCCAGAGAGC TGACAGGCTT GGAGGACTAC CTGCTGCTTG CCTGTGACGG TTTCTTCGAT 
GTCGTCCCCC ACCATGAAAT CCCGGGTCTT GTCCATGGCC ACTTGCTCAG GCAGAAGGGC 
AGTGGGATGC ACGTTGCTGA GGAGCTGGTG GCTGTAGCCC GTGACCGGGG TTCCCATGAC 
AACATTACAG TCATGGTGGT TTTCCTTAGG GACCCCCTGG AGCTGCTGGA GGGTGGGGGG 
CAGGGGGCAG GGGGTGCCCA GGCAGATGTA GGAAGTCAGG ACTTGTCCAC TGGCCTCTCA 
GAACTTGAGA TCAATACCTC ACAGAGAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppm1f ( XM_008768853.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767075.1 Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa44651
Clone ID Related Accession (Same CDS sequence)	XM_008768854.2 , XM_008768855.1 , XM_008768853.2
Accession Version	XM_008768853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTG GAGCCCCACA GAACAGCAGC CAGATGGCCT GTGACGGCGA GATCCCAGGT 
TTCCTGGACA CACTCCTCCA AGACTTCCCA GCCCCACTGA GCCTGGAGAG CCCTTTGCCA 
TGGAAGGTCC CAGGGACAGT CCTGGGCCAG GAGGAGGTGG AGGCTGAGCT GACGGAGCTG 
GCAATGGGTT TCCTGGGCAG CAGGAATGCA CCCCCAGCAG TTGCTGCGGC TGTGACCCAC 
GAGGCAATTT CCCAGCTGCT TCAGACAGAC CTCTCTGAAT TTAAGAGGTT GCCTGAACAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGAGA GTCCTCACGA CCCTACTGGA TGCCAAAGGC 
CTGTCCCGGA GCTTCTTTAA CTGTCTTTGG GAAGTATGCA GCCAGTGGCA GAAACGGGTG 
CCACTGACTG CCCAGGCCCC TCAGCGCAAG TGGCTGGTCT CTATCCATGC CATCCGGAAC 
ACACGACGAA AGATGGAGGA TCGACACGTG TCCCTTCCTG CTTTCAACCA CCTCTTCGGC 
TTGTCTGACT CCGTGCACCG CGCCTACTTC GCTGTGTTTG ATGGTCATGG AGGTGTGGAT 
GCAGCACGGT ATGCCTCTGT CCACGTGCAC ACCAACGCCT CTCACCAGCC AGAGCTGCTC 
ACAGACCCTG CAGCAGCTCT CAAAGAAGCC TTCCGGCATA CTGACCAGAT GTTTCTCCAG 
AAAGCCAAGC GAGAGCGACT GCAGAGCGGT ACCACAGGTG TGTGTGCGCT CATCACAGGA 
GCAGCCCTGC ATGTTGCCTG GCTTGGAGAC TCCCAGGTCA TCCTGGTACA GCAAGGACAG 
GTAGTGAAGC TGATGGAGCC ACACAAACCG GAACGACAGG ATGAGAAATC ACGCATTGAA 
GCGCTGGGCG GCTTTGTTTC TCTCATGGAC TGCTGGAGAG TCAACGGGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGGGATGT CTTCCAGAAG CCTTATGTGT CTGGCGAGGC AGATGCAGCA 
TCCAGAGAGC TGACAGGCTT GGAGGACTAC CTGCTGCTTG CCTGTGACGG TTTCTTCGAT 
GTCGTCCCCC ACCATGAAAT CCCGGGTCTT GTCCATGGCC ACTTGCTCAG GCAGAAGGGC 
AGTGGGATGC ACGTTGCTGA GGAGCTGGTG GCTGTAGCCC GTGACCGGGG TTCCCATGAC 
AACATTACAG TCATGGTGGT TTTCCTTAGG GACCCCCTGG AGCTGCTGGA GGGTGGGGGG 
CAGGGGGCAG GGGGTGCCCA GGCAGATGTA GGAAGTCAGG ACTTGTCCAC TGGCCTCTCA 
GAACTTGAGA TCAATACCTC ACAGAGAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767075.1
CDS	173..1705
Translation	MASGAPQNSSQMACDGEIPGFLDTLLQDFPAPLSLESPLPWKVPGTVLGQEEVEAELTELAMGFLGSRNAPPAVAAAVTHEAISQLLQTDLSEFKRLPEQEEEEEEEEERVLTTLLDAKGLSRSFFNCLWEVCSQWQKRVPLTAQAPQRKWLVSIHAIRNTRRKMEDRHVSLPAFNHLFGLSDSVHRAYFAVFDGHGGVDAARYASVHVHTNASHQPELLTDPAAALKEAFRHTDQMFLQKAKRERLQSGTTGVCALITGAALHVAWLGDSQVILVQQGQVVKLMEPHKPERQDEKSRIEALGGFVSLMDCWRVNGTLAVSRAIGKTLAVSRAIGKTLAVSRAIGKTLAVSRAIGKTLAVSRAIGKTLAVSRAIGKTLAVSRAIGDVFQKPYVSGEADAASRELTGLEDYLLLACDGFFDVVPHHEIPGLVHGHLLRQKGSGMHVAEELVAVARDRGSHDNITVMVVFLRDPLELLEGGGQGAGGAQADVGSQDLSTGLSELEINTSQRS

Target ORF information:

RefSeq Version	XM_008768853.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1F (Ppm1f), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768853.2

ORF Insert Sequence:

ATGGCCTCTG GAGCCCCACA GAACAGCAGC CAGATGGCCT GTGACGGCGA GATCCCAGGT 
TTCCTGGACA CACTCCTCCA AGACTTCCCA GCCCCACTGA GCCTGGAGAG CCCTTTGCCA 
TGGAAGGTCC CAGGGACAGT CCTGGGCCAG GAGGAGGTGG AGGCTGAGCT GACGGAGCTG 
GCAATGGGTT TCCTGGGCAG CAGGAATGCA CCCCCAGCAG TTGCTGCGGC TGTGACCCAC 
GAGGCAATTT CCCAGCTGCT TCAGACAGAC CTCTCTGAAT TTAAGAGGTT GCCTGAACAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGAGA GTCCTCACGA CCCTACTGGA TGCCAAAGGC 
CTGTCCCGGA GCTTCTTTAA CTGTCTTTGG GAAGTATGCA GCCAGTGGCA GAAACGGGTG 
CCACTGACTG CCCAGGCCCC TCAGCGCAAG TGGCTGGTCT CTATCCATGC CATCCGGAAC 
ACACGACGAA AGATGGAGGA TCGACACGTG TCCCTTCCTG CTTTCAACCA CCTCTTCGGC 
TTGTCTGACT CCGTGCACCG CGCCTACTTC GCTGTGTTTG ATGGTCATGG AGGTGTGGAT 
GCAGCACGGT ATGCCTCTGT CCACGTGCAC ACCAACGCCT CTCACCAGCC AGAGCTGCTC 
ACAGACCCTG CAGCAGCTCT CAAAGAAGCC TTCCGGCATA CTGACCAGAT GTTTCTCCAG 
AAAGCCAAGC GAGAGCGACT GCAGAGCGGT ACCACAGGTG TGTGTGCGCT CATCACAGGA 
GCAGCCCTGC ATGTTGCCTG GCTTGGAGAC TCCCAGGTCA TCCTGGTACA GCAAGGACAG 
GTAGTGAAGC TGATGGAGCC ACACAAACCG GAACGACAGG ATGAGAAATC ACGCATTGAA 
GCGCTGGGCG GCTTTGTTTC TCTCATGGAC TGCTGGAGAG TCAACGGGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG TCCAGAGCCA TTGGTAAGAC CCTGGCAGTG 
TCCAGAGCCA TTGGGGATGT CTTCCAGAAG CCTTATGTGT CTGGCGAGGC AGATGCAGCA 
TCCAGAGAGC TGACAGGCTT GGAGGACTAC CTGCTGCTTG CCTGTGACGG TTTCTTCGAT 
GTCGTCCCCC ACCATGAAAT CCCGGGTCTT GTCCATGGCC ACTTGCTCAG GCAGAAGGGC 
AGTGGGATGC ACGTTGCTGA GGAGCTGGTG GCTGTAGCCC GTGACCGGGG TTCCCATGAC 
AACATTACAG TCATGGTGGT TTTCCTTAGG GACCCCCTGG AGCTGCTGGA GGGTGGGGGG 
CAGGGGGCAG GGGGTGCCCA GGCAGATGTA GGAAGTCAGG ACTTGTCCAC TGGCCTCTCA 
GAACTTGAGA TCAATACCTC ACAGAGAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-149 represses metastasis of hepatocellular carcinoma by targeting actin-regulatory proteins PPM1F.
Oncotarget6(35)37808-23(2015 Nov)
Luo G,Chao YL,Tang B,Li BS,Xiao YF,Xie R,Wang SM,Wu YY,Dong H,Liu XD,Yang SM

POPX2 phosphatase regulates the KIF3 kinesin motor complex.
Journal of cell science127(Pt 4)727-39(2014 Feb)
Phang HQ,Hoon JL,Lai SK,Zeng Y,Chiam KH,Li HY,Koh CG

An active C-terminally truncated form of Ca (2+) /calmodulin-dependent protein kinase phosphatase-N (CaMKP-N/PPM1E).
BioMed research international2013134813(2013)
Ishida A,Tsumura K,Oue M,Takenaka Y,Shigeri Y,Goshima N,Ishihara Y,Hirano T,Baba H,Sueyoshi N,Kameshita I,Yamazaki T

Ppm1E is an in cellulo AMP-activated protein kinase phosphatase.
Cellular signalling23(1)114-24(2011 Jan)
Voss M,Paterson J,Kelsall IR,Mart?n-Granados C,Hastie CJ,Peggie MW,Cohen PT

The POPX2 phosphatase regulates cancer cell motility and invasiveness.
Cell cycle (Georgetown, Tex.)9(1)179-87(2010 Jan)
Susila A,Chan H,Loh AX,Phang HQ,Wong ET,Tergaonkar V,Koh CG

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Ppm1f cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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