Gnat3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gnat3
Entrez Gene ID	286924
Full Name	G protein subunit alpha transducin 3
Synonyms	gnat-3
General protein information	Preferred Names G protein subunit alpha transducin 3 Names guanine nucleotide-binding protein G(t) subunit alpha-3 guanine nucleotide binding protein, alpha transducing 3 gustducin alpha-3 chain
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q11
Summary	G protein alpha-subunit of taste tissue [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_173139.1	NP_775162.1	guanine nucleotide-binding protein G(t) subunit alpha-3

Pathways from BioSystems

Homologies

Homo sapiens (human)	GNAT3	NP_001095856.1
Mus musculus (house mouse)	Gnat3	NP_001074612.1
Arabidopsis thaliana (thale cress)	GP ALPHA 1	NP_180198.1
Pan troglodytes (chimpanzee)	GNAT3	XP_527800.2
	GNAT3	XP_001108504.1
Canis lupus familiaris (dog)	GNAT3	XP_849666.1
Gallus gallus (chicken)	GNAT3	NP_001254740.1
Bos taurus (cattle)	GNAT3	NP_001103452.1
Oryza sativa (rice)	Os05g0333200	NP_001055226.1
Rattus norvegicus (Norway rat)	Gnat3	NP_775162.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of sweet taste receptor and gut hormone secretion in modelled type 2 diabetes.
Feng R, Qian C, Liu Q, Jin Y, Liu L, Li S, Liao Y, Zhou H, Liu W, Rayner CK, Ma J
General and comparative endocrinology252142-149(2017 Oct)

Genetic labeling of Tas1r1 and Tas2r131 taste receptor cells in mice.
Voigt A, H?bner S, Lossow K, Hermans-Borgmeyer I, Boehm U, Meyerhof W
Chemical senses37(9)897-911(2012 Nov)

Cholinergic chemosensory cells in the trachea regulate breathing.
Krasteva G, Canning BJ, Hartmann P, Veres TZ, Papadakis T, M?hlfeld C, Schliecker K, Tallini YN, Braun A, Hackstein H, Baal N, Weihe E, Sch?tz B, Kotlikoff M, Ibanez-Tallon I, Kummer W
Proceedings of the National Academy of Sciences of the United States of America108(23)9478-83(2011 Jun)

Fixation conditions affect the immunoreactivity of gustducin in rat vallate taste buds.
Haga M, Yoshie S
Archives of histology and cytology73(2)91-3(2010)

Expression of alpha-gustducin in the circumvallate papillae of taste buds of diabetic rats.
Zhou LH, Liu XM, Feng XH, Han LO, Liu GD
Acta histochemica111(2)145-9(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sweet taste molecules of T1R2, T1R3, alpha-gustducin and TRPM5 in ileum were dramatically higher than those in duodenum (P<0.01 for each).
Title: Expression of sweet taste receptor and gut hormone secretion in modelled type 2 diabetes.

The staining protocols, especially the fixation time, cause discrepancies in gustducin immunoreactivities.
Title: Fixation conditions affect the immunoreactivity of gustducin in rat vallate taste buds.

Data show that the positive expression of alpha-gustducin (Gnat3 protein in diabetic rat taste bud cells is higher than that in normal controls as shown by both immunohistochemistry and RT-PCR.
Title: Expression of alpha-gustducin in the circumvallate papillae of taste buds of diabetic rats.

The data suggest that gustducin is involved in signal transduction of all the tastes of sweet, umami, and bitter in the soft palate, in contrast to its limited function in the tongue.
Title: Expression of gustducin overlaps with that of type III IP3 receptor in taste buds of the rat soft palate.

Axonemal-associated localization within the midpiece and principal piece of rat spermatozoa raises the possibility that this G protein alpha-subunit may process intracellular signals controlling sperm motility.
Title: Expression of the G-protein alpha-subunit gustducin in mammalian spermatozoa.

The following Gnat3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gnat3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13911	NM_173139.1 Latest version!	Rattus norvegicus G protein subunit alpha transducin 3 (Gnat3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gnat3 ( NM_173139.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_775162.1 Rattus norvegicus G protein subunit alpha transducin 3 (Gnat3), mRNA.

Price & Availability↑

CloneID	ORa13911
Clone ID Related Accession (Same CDS sequence)	NM_173139.1
Accession Version	NM_173139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein G(t) subunit alpha-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X65747.1. On Dec 17, 2003 this sequence version replaced XM_346682.1. Sequence Note: This sequence has been modified as follows: removed 35 bp suspected to be vector contamination from the 3' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X65747.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04563757, SAMN04563759 [ECO:0000348] ##Evidence-Data-END##

ATGGGAAGTG GAATTAGTTC AGAGAGCAAG GAGTCAGCCA AAAGGTCCAA AGAACTGGAG 
AAGAAGCTTC AGGAAGATGC TGAACGAGAT GCAAGAACTG TGAAGTTGCT GCTATTAGGA 
GCAGGTGAAT CTGGAAAAAG TACTATTGTT AAACAAATGA AGATCATCCA CAAGAATGGT 
TACAGTAAAC AAGAATGCAT GGAGTTTAAA GCAGTGGTTT ACAGTAACAC GTTGCAGTCC 
ATCCTGGCCA TTGTGAAAGC CATGACTACA CTAGGGATTG ATTATGTCAA TCCGAGAAGT 
AGAGAGGACC AACAACTGCT TCTCTCCATG GCAAACACAC TAGAAGATGG TGACATGACG 
CCTCAGTTGG CTGAAATAAT TAAACGTCTG TGGGGCGATC CAGGAATTCA AGCCTGCTTC 
GAAAGGGCAT CTGAATACCA GCTCAATGAC TCTGCAGCTT ACTACCTTAA TGACTTAGAT 
AGACTCACAG CCCCTGGGTA TGTGCCAAAT GAACAAGACG TTCTACATTC CCGGGTGAAA 
ACCACTGGTA TCATTGAAAC TCAATTCTCC TTTAAAGACT TGAACTTCAG AATGTTTGAT 
GTAGGTGGCC AGAGATCAGA AAGAAAGAAA TGGATCCACT GCTTTGAAGG AGTGACGTGC 
ATTATATTTT GTGCAGCCCT AAGTGCCTAC GACATGGTAC TTGTAGAAGA TGAAGAGGTG 
AACAGAATGC ATGAAAGTCT TCACCTCTTC AACAGCATCT GTAATCACAA GTATTTTGCA 
ACCACCTCCA TTGTTCTGTT TCTTAACAAG AAAGATCTCT TCCAGGAGAA AGTGACCAAG 
GTGCACCTCA GCATCTGTTT CCCAGAATAC ACTGGACCAA ATACATTCGA AGATGCAGGG 
AACTACATCA AGAACCAGTT CCTAGACCTG AACTTAAAAA AAGAAGATAA GGAAATCTAT 
TCTCACATGA CCTGCGCTAC TGACACACAA AACGTCAAAT TCGTGTTTGA TGCCGTGACA 
GATATAATAA TAAAAGAGAA CCTCAAAGAC TGTGGGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775162.1
CDS	114..1178
Translation	MGSGISSESKESAKRSKELEKKLQEDAERDARTVKLLLLGAGESGKSTIVKQMKIIHKNGYSKQECMEFKAVVYSNTLQSILAIVKAMTTLGIDYVNPRSREDQQLLLSMANTLEDGDMTPQLAEIIKRLWGDPGIQACFERASEYQLNDSAAYYLNDLDRLTAPGYVPNEQDVLHSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFCAALSAYDMVLVEDEEVNRMHESLHLFNSICNHKYFATTSIVLFLNKKDLFQEKVTKVHLSICFPEYTGPNTFEDAGNYIKNQFLDLNLKKEDKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	NM_173139.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit alpha transducin 3 (Gnat3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173139.1

ORF Insert Sequence:

ATGGGAAGTG GAATTAGTTC AGAGAGCAAG GAGTCAGCCA AAAGGTCCAA AGAACTGGAG 
AAGAAGCTTC AGGAAGATGC TGAACGAGAT GCAAGAACTG TGAAGTTGCT GCTATTAGGA 
GCAGGTGAAT CTGGAAAAAG TACTATTGTT AAACAAATGA AGATCATCCA CAAGAATGGT 
TACAGTAAAC AAGAATGCAT GGAGTTTAAA GCAGTGGTTT ACAGTAACAC GTTGCAGTCC 
ATCCTGGCCA TTGTGAAAGC CATGACTACA CTAGGGATTG ATTATGTCAA TCCGAGAAGT 
AGAGAGGACC AACAACTGCT TCTCTCCATG GCAAACACAC TAGAAGATGG TGACATGACG 
CCTCAGTTGG CTGAAATAAT TAAACGTCTG TGGGGCGATC CAGGAATTCA AGCCTGCTTC 
GAAAGGGCAT CTGAATACCA GCTCAATGAC TCTGCAGCTT ACTACCTTAA TGACTTAGAT 
AGACTCACAG CCCCTGGGTA TGTGCCAAAT GAACAAGACG TTCTACATTC CCGGGTGAAA 
ACCACTGGTA TCATTGAAAC TCAATTCTCC TTTAAAGACT TGAACTTCAG AATGTTTGAT 
GTAGGTGGCC AGAGATCAGA AAGAAAGAAA TGGATCCACT GCTTTGAAGG AGTGACGTGC 
ATTATATTTT GTGCAGCCCT AAGTGCCTAC GACATGGTAC TTGTAGAAGA TGAAGAGGTG 
AACAGAATGC ATGAAAGTCT TCACCTCTTC AACAGCATCT GTAATCACAA GTATTTTGCA 
ACCACCTCCA TTGTTCTGTT TCTTAACAAG AAAGATCTCT TCCAGGAGAA AGTGACCAAG 
GTGCACCTCA GCATCTGTTT CCCAGAATAC ACTGGACCAA ATACATTCGA AGATGCAGGG 
AACTACATCA AGAACCAGTT CCTAGACCTG AACTTAAAAA AAGAAGATAA GGAAATCTAT 
TCTCACATGA CCTGCGCTAC TGACACACAA AACGTCAAAT TCGTGTTTGA TGCCGTGACA 
GATATAATAA TAAAAGAGAA CCTCAAAGAC TGTGGGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of sweet taste receptor and gut hormone secretion in modelled type 2 diabetes.
General and comparative endocrinology252142-149(2017 Oct)
Feng R,Qian C,Liu Q,Jin Y,Liu L,Li S,Liao Y,Zhou H,Liu W,Rayner CK,Ma J

Genetic labeling of Tas1r1 and Tas2r131 taste receptor cells in mice.
Chemical senses37(9)897-911(2012 Nov)
Voigt A,H?bner S,Lossow K,Hermans-Borgmeyer I,Boehm U,Meyerhof W

Cholinergic chemosensory cells in the trachea regulate breathing.
Proceedings of the National Academy of Sciences of the United States of America108(23)9478-83(2011 Jun)
Krasteva G,Canning BJ,Hartmann P,Veres TZ,Papadakis T,M?hlfeld C,Schliecker K,Tallini YN,Braun A,Hackstein H,Baal N,Weihe E,Sch?tz B,Kotlikoff M,Ibanez-Tallon I,Kummer W

Fixation conditions affect the immunoreactivity of gustducin in rat vallate taste buds.
Archives of histology and cytology73(2)91-3(2010)
Haga M,Yoshie S

Expression of alpha-gustducin in the circumvallate papillae of taste buds of diabetic rats.
Acta histochemica111(2)145-9(2009)
Zhou LH,Liu XM,Feng XH,Han LO,Liu GD