Rpl8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rpl8
Entrez Gene ID	26962
Full Name	ribosomal protein L8
General protein information	Preferred Names ribosomal protein L8 Names 60S ribosomal protein L8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	human homolog is a 60S ribosomal protein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034916.2	NP_001030088.2	60S ribosomal protein L8

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rpl8	NP_001030088.2
Arabidopsis thaliana (thale cress)	AT4G36130	NP_195336.1
Rattus norvegicus (Norway rat)	LOC100360117	XP_002729189.1
Arabidopsis thaliana (thale cress)	EMB2296	NP_179393.1
Rattus norvegicus (Norway rat)	LOC100910370	XP_003750416.1
Oryza sativa (rice)	Os12g0567700	NP_001067062.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural snapshots of actively translating human ribosomes.
Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, B?rger J, Mielke T, Scheerer P, Spahn CM
Cell161(4)845-57(2015 May)

Structures of the human and Drosophila 80S ribosome.
Anger AM, Armache JP, Berninghausen O, Habeck M, Subklewe M, Wilson DN, Beckmann R
Nature497(7447)80-5(2013 May)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Comparative study of human and mouse postsynaptic proteomes finds high compositional conservation and abundance differences for key synaptic proteins.
Bay?s A, Collins MO, Croning MD, van de Lagemaat LN, Choudhary JS, Grant SG
PloS one7(10)e46683(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rpl8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rpl8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08960	NM_001034916.2 Latest version!	Rattus norvegicus ribosomal protein L8 (Rpl8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rpl8 ( NM_001034916.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001030088.2 Rattus norvegicus ribosomal protein L8 (Rpl8), mRNA.

Price & Availability↑

CloneID	ORa08960
Clone ID Related Accession (Same CDS sequence)	NM_001034916.2
Accession Version	NM_001034916.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	60S ribosomal protein L8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CF111102.1. On Oct 28, 2005 this sequence version replaced NM_001034916.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF111102.1, BC126063.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCGTG TGATCCGAGG CCAGAGGAAA GGTGCCGGTT CTGTGTTTCG TGCGCACGTG 
AAGCACCGTA AGGGAGCCGC GCGTCTGCGT GCTGTGGACT TCGCGGAGCG ACACGGCTAC 
ATTAAAGGCA TCGTAAAGGA CATCATTCAT GACCCTGGCC GCGGCGCTCC CCTCGCGAAA 
GTAGTCTTTC GTGATCCCTA TCGATTCAAG AAGCGGACAG AGCTGTTCAT CGCCGCAGAG 
GGAATCCACA CTGGACAGTT TGTGTACTGC GGCAAGAAGG CCCAGCTGAA TATTGGCAAT 
GTTTTGCCCG TGGGCACCAT GCCTGAGGGT ACTATCGTGT GTTGTCTGGA GGAGAAGCCT 
GGGGACAGGG GCAAGCTGGC ACGAGCCTCC GGGAACTATG CTACAGTCAT CTCCCACAAC 
CCAGAGACCA AGAAGACCCG AGTGAAGCTG CCTTCAGGGT CCAAGAAGGT CATTTCCTCT 
GCTAACCGAG CTGTTGTTGG TGTCGTGGCT GGCGGGGGCA GAATTGACAA GCCTATCTTA 
AAGGCTGGCC GTGCCTACCA TAAGTACAAG GCAAAGAGGA ACTGCTGGCC ACGTGTGCGG 
GGTGTTGCCA TGAATCCTGT GGAGCATCCC TTTGGCGGTG GTAACCACCA GCACATTGGC 
AAGCCTTCCA CTATCCGAAG AGATGCCCCA GCTGGGCGCA AAGTGGGTCT CATTGCTGCT 
CGCCGGACTG GACGGCTACG TGGAACCAAA ACTGTACAGG AGAAGGAGAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030088.2
CDS	36..809
Translation	MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQEKEN

Target ORF information:

RefSeq Version	NM_001034916.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ribosomal protein L8 (Rpl8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034916.2

ORF Insert Sequence:

ATGGGCCGTG TGATCCGAGG CCAGAGGAAA GGTGCCGGTT CTGTGTTTCG TGCGCACGTG 
AAGCACCGTA AGGGAGCCGC GCGTCTGCGT GCTGTGGACT TCGCGGAGCG ACACGGCTAC 
ATTAAAGGCA TCGTAAAGGA CATCATTCAT GACCCTGGCC GCGGCGCTCC CCTCGCGAAA 
GTAGTCTTTC GTGATCCCTA TCGATTCAAG AAGCGGACAG AGCTGTTCAT CGCCGCAGAG 
GGAATCCACA CTGGACAGTT TGTGTACTGC GGCAAGAAGG CCCAGCTGAA TATTGGCAAT 
GTTTTGCCCG TGGGCACCAT GCCTGAGGGT ACTATCGTGT GTTGTCTGGA GGAGAAGCCT 
GGGGACAGGG GCAAGCTGGC ACGAGCCTCC GGGAACTATG CTACAGTCAT CTCCCACAAC 
CCAGAGACCA AGAAGACCCG AGTGAAGCTG CCTTCAGGGT CCAAGAAGGT CATTTCCTCT 
GCTAACCGAG CTGTTGTTGG TGTCGTGGCT GGCGGGGGCA GAATTGACAA GCCTATCTTA 
AAGGCTGGCC GTGCCTACCA TAAGTACAAG GCAAAGAGGA ACTGCTGGCC ACGTGTGCGG 
GGTGTTGCCA TGAATCCTGT GGAGCATCCC TTTGGCGGTG GTAACCACCA GCACATTGGC 
AAGCCTTCCA CTATCCGAAG AGATGCCCCA GCTGGGCGCA AAGTGGGTCT CATTGCTGCT 
CGCCGGACTG GACGGCTACG TGGAACCAAA ACTGTACAGG AGAAGGAGAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural snapshots of actively translating human ribosomes.
Cell161(4)845-57(2015 May)
Behrmann E,Loerke J,Budkevich TV,Yamamoto K,Schmidt A,Penczek PA,Vos MR,B?rger J,Mielke T,Scheerer P,Spahn CM

Structures of the human and Drosophila 80S ribosome.
Nature497(7447)80-5(2013 May)
Anger AM,Armache JP,Berninghausen O,Habeck M,Subklewe M,Wilson DN,Beckmann R

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Comparative study of human and mouse postsynaptic proteomes finds high compositional conservation and abundance differences for key synaptic proteins.
PloS one7(10)e46683(2012)
Bay?s A,Collins MO,Croning MD,van de Lagemaat LN,Choudhary JS,Grant SG