Hck cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hck
Entrez Gene ID	25734
Full Name	HCK proto-oncogene, Src family tyrosine kinase
Synonyms	Hctk,p56Hck,p59Hck
General protein information	Preferred Names HCK proto-oncogene, Src family tyrosine kinase Names tyrosine-protein kinase HCK hematopoietic cell kinase hemopoietic cell kinase hemopoietic cell tyrosine kinase p56-HCK
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41
Summary	The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types.The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms in human and mouse. Based on strong sequence conservation amongst these species, this may also be true for rat. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013185.3	NP_037317.2	tyrosine-protein kinase HCK

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HCK	XP_514571.3
Canis lupus familiaris (dog)	HCK	XP_542952.2
Bos taurus (cattle)	HCK	NP_001103664.1
Gallus gallus (chicken)	HCK	XP_004947228.1
Danio rerio (zebrafish)	LOC101885596	XP_005174178.1
Homo sapiens (human)	HCK	NP_002101.2
Rattus norvegicus (Norway rat)	Hck	NP_037317.2
Mus musculus (house mouse)	Hck	NP_034537.2
Xenopus tropicalis (tropical clawed frog)	hck	NP_001015722.1
	LOC715723	XP_002798221.1

Gene Ontology
Bibliography

Related articles in PubMed

Hck Promotes Neuronal Apoptosis Following Intracerebral Hemorrhage.
Wang J, Chen R, Liu X, Shen J, Yan Y, Gao Y, Tao T, Shi J
Cellular and molecular neurobiology37(2)251-261(2017 Mar)

Tarp regulates early Chlamydia-induced host cell survival through interactions with the human adaptor protein SHC1.
Mehlitz A, Banhart S, M?urer AP, Kaushansky A, Gordus AG, Zielecki J, Macbeath G, Meyer TF
The Journal of cell biology190(1)143-57(2010 Jul)

Regulation of p73 by Hck through kinase-dependent and independent mechanisms.
Paliwal P, Radha V, Swarup G
BMC molecular biology845(2007 May)

Differential kinase requirements in human and mouse Fc-gamma receptor phagocytosis and endocytosis.
Huang ZY, Barreda DR, Worth RG, Indik ZK, Kim MK, Chien P, Schreiber AD
Journal of leukocyte biology80(6)1553-62(2006 Dec)

Activation of the lysosome-associated p61Hck isoform triggers the biogenesis of podosomes.
Cougoule C, Carr?no S, Castandet J, Labrousse A, Astarie-Dequeker C, Poincloux R, Le Cabec V, Maridonneau-Parini I
Traffic (Copenhagen, Denmark)6(8)682-94(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hck was up-regulated in neurons adjacent to the hematoma after intracerebral hemorrhage.
Title: Hck Promotes Neuronal Apoptosis Following Intracerebral Hemorrhage.

Alternate protein isoforms arise through the use of a non-AUG (CUG), and a downstream in-frame AUG translation initiation codons, in mouse and human. Same is likely true for rat as there is strong sequence conservation amongst different species.
Title: Two isoforms of murine hck, generated by utilization of alternative translational initiation codons, exhibit different patterns of subcellular localization.

The following Hck gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hck cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12146	NM_013185.3 Latest version!	Rattus norvegicus HCK proto-oncogene, Src family tyrosine kinase (Hck), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hck ( NM_013185.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037317.2 Rattus norvegicus HCK proto-oncogene, Src family tyrosine kinase (Hck), mRNA.

Price & Availability↑

CloneID	ORa12146
Clone ID Related Accession (Same CDS sequence)	NM_013185.3
Accession Version	NM_013185.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein kinase HCK
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC078890.1. On Jan 22, 2011 this sequence version replaced NM_013185.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078890.1, M83666.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689594 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 10967098, 1875927 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGGGGGTC GTTCGAGCTG CGAGGATCCG GGCTGCCCGC GAGGCGAAGG GCGGGTGCCC 
AGGATGGGAT GTGTGAAGTC CAGGTTCCTC CGAGAAGGAA GCAAGGCCTC AAAAATAGAG 
CCAAATGCCA ACCAGAAAGG CCCTGTGTAT GTGCCGGATC CCACGTCCCC TAAGAAGCTG 
GGACCGAACA GCATCAACAG CCTGCCCCCG GGGGTCGTGG AGGGCTCTGA GGACACCATT 
GTGGTCGCAC TGTACGACTA TGAGGCCATT CACCGTGAAG ACCTCAGCTT CCAGAAGGGA 
GACCAGATGG TGGTTCTGGA GGAGTCTGGG GAGTGGTGGA AGGCCCGTTC CCTGGCTACC 
AAGAAAGAAG GCTATATCCC AAGCAATTAT GTAGCTCGAG TTAACTCTTT GGAGACTGAG 
GAGTGGTTCT TCAAGGGTAT CAGCCGGAAG GATGCAGAGC GCCACCTGCT GGCTCCCGGG 
AACATGCTGG GCTCCTTCAT GATCCGGGAC AGTGAGACCA CCAAAGGGAG CTACTCACTT 
TCTGTTCGAG ACTTTGACCC CCAGCACGGA GACACGGTGA AGCATTATAA AATCCGGACA 
CTGGACAGTG GAGGCTTCTA CATCTCTCCG AGGAGCACCT TCAGCAGCCT GCAGGAACTT 
GTCGTCCACT ACAAGAAGGG GAAGGATGGG CTCTGCCAGA AGCTGTCAGT GCCCTGTGTG 
TCTCCCAAAC CCCAGAAGCC ATGGGAGAAA GATGCCTGGG AGATTCCTCG AGAATCCCTC 
CAGATGGAGA AGAAACTGGG AGCCGGGCAG TTTGGAGAAG TGTGGATGGC CACCTACAAC 
AAGCACACCA AAGTGGCGGT GAAGACAATG AAGCCAGGGA GCATGTCTGT GGAGGCCTTC 
CTGGCAGAGG CCAACCTGAT GAAGACGTTA CAGCATGATA AACTGGTGAA GCTGCACGCC 
GTGGTCTCTC AGGAGCCCAT CTTTATTGTC ACCGAGTTCA TGGCCAAAGG AAGCCTGCTG 
GACTTTCTCA AGAGTGAAGA AGGCAGCAAG CAGCCACTGC CAAAACTCAT TGACTTCTCA 
GCCCAGATTT CAGAGGGCAT GGCTTTCATT GAGCAGAGGA ACTACATCCA CCGAGACCTC 
CGGGCTGCCA ACATCTTGGT TTCTGCATCA CTGGTGTGTA AGATCGCTGA CTTTGGACTG 
GCACGGATCA TCGAGGACAA TGAGTACACA GCTCGGGAAG GAGCCAAGTT CCCCATCAAG 
TGGACAGCTC CTGAAGCCAT CAACTTTGGC TCCTTCACCA TCAAGTCAGA TGTCTGGTCC 
TTTGGTATCC TGCTGATGGA AATCGTCACC TACGGCCGGA TCCCTTACCC AGGTATGTCA 
AACCCAGAGG TGATTCGAGC ACTAGAGCAT GGGTACCGTA TGCCTCGACC AGATAACTGC 
CCAGAGGAGC TCTACAGTAT CATGATCCGC TGCTGGAAGA ACCGTCCAGA GGAACGGCCC 
ACTTTCGAAT ACATCCAGAG CGTGCTGGAT GACTTCTACA CGGCCACTGA GAGCCAGTAT 
CAGCAGCAAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037317.2
CDS	199..1773
Translation	LGGRSSCEDPGCPRGEGRVPRMGCVKSRFLREGSKASKIEPNANQKGPVYVPDPTSPKKLGPNSINSLPPGVVEGSEDTIVVALYDYEAIHREDLSFQKGDQMVVLEESGEWWKARSLATKKEGYIPSNYVARVNSLETEEWFFKGISRKDAERHLLAPGNMLGSFMIRDSETTKGSYSLSVRDFDPQHGDTVKHYKIRTLDSGGFYISPRSTFSSLQELVVHYKKGKDGLCQKLSVPCVSPKPQKPWEKDAWEIPRESLQMEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANLMKTLQHDKLVKLHAVVSQEPIFIVTEFMAKGSLLDFLKSEEGSKQPLPKLIDFSAQISEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARIIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALEHGYRMPRPDNCPEELYSIMIRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP

Target ORF information:

RefSeq Version	NM_013185.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HCK proto-oncogene, Src family tyrosine kinase (Hck), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013185.3

ORF Insert Sequence:

CTGGGGGGTC GTTCGAGCTG CGAGGATCCG GGCTGCCCGC GAGGCGAAGG GCGGGTGCCC 
AGGATGGGAT GTGTGAAGTC CAGGTTCCTC CGAGAAGGAA GCAAGGCCTC AAAAATAGAG 
CCAAATGCCA ACCAGAAAGG CCCTGTGTAT GTGCCGGATC CCACGTCCCC TAAGAAGCTG 
GGACCGAACA GCATCAACAG CCTGCCCCCG GGGGTCGTGG AGGGCTCTGA GGACACCATT 
GTGGTCGCAC TGTACGACTA TGAGGCCATT CACCGTGAAG ACCTCAGCTT CCAGAAGGGA 
GACCAGATGG TGGTTCTGGA GGAGTCTGGG GAGTGGTGGA AGGCCCGTTC CCTGGCTACC 
AAGAAAGAAG GCTATATCCC AAGCAATTAT GTAGCTCGAG TTAACTCTTT GGAGACTGAG 
GAGTGGTTCT TCAAGGGTAT CAGCCGGAAG GATGCAGAGC GCCACCTGCT GGCTCCCGGG 
AACATGCTGG GCTCCTTCAT GATCCGGGAC AGTGAGACCA CCAAAGGGAG CTACTCACTT 
TCTGTTCGAG ACTTTGACCC CCAGCACGGA GACACGGTGA AGCATTATAA AATCCGGACA 
CTGGACAGTG GAGGCTTCTA CATCTCTCCG AGGAGCACCT TCAGCAGCCT GCAGGAACTT 
GTCGTCCACT ACAAGAAGGG GAAGGATGGG CTCTGCCAGA AGCTGTCAGT GCCCTGTGTG 
TCTCCCAAAC CCCAGAAGCC ATGGGAGAAA GATGCCTGGG AGATTCCTCG AGAATCCCTC 
CAGATGGAGA AGAAACTGGG AGCCGGGCAG TTTGGAGAAG TGTGGATGGC CACCTACAAC 
AAGCACACCA AAGTGGCGGT GAAGACAATG AAGCCAGGGA GCATGTCTGT GGAGGCCTTC 
CTGGCAGAGG CCAACCTGAT GAAGACGTTA CAGCATGATA AACTGGTGAA GCTGCACGCC 
GTGGTCTCTC AGGAGCCCAT CTTTATTGTC ACCGAGTTCA TGGCCAAAGG AAGCCTGCTG 
GACTTTCTCA AGAGTGAAGA AGGCAGCAAG CAGCCACTGC CAAAACTCAT TGACTTCTCA 
GCCCAGATTT CAGAGGGCAT GGCTTTCATT GAGCAGAGGA ACTACATCCA CCGAGACCTC 
CGGGCTGCCA ACATCTTGGT TTCTGCATCA CTGGTGTGTA AGATCGCTGA CTTTGGACTG 
GCACGGATCA TCGAGGACAA TGAGTACACA GCTCGGGAAG GAGCCAAGTT CCCCATCAAG 
TGGACAGCTC CTGAAGCCAT CAACTTTGGC TCCTTCACCA TCAAGTCAGA TGTCTGGTCC 
TTTGGTATCC TGCTGATGGA AATCGTCACC TACGGCCGGA TCCCTTACCC AGGTATGTCA 
AACCCAGAGG TGATTCGAGC ACTAGAGCAT GGGTACCGTA TGCCTCGACC AGATAACTGC 
CCAGAGGAGC TCTACAGTAT CATGATCCGC TGCTGGAAGA ACCGTCCAGA GGAACGGCCC 
ACTTTCGAAT ACATCCAGAG CGTGCTGGAT GACTTCTACA CGGCCACTGA GAGCCAGTAT 
CAGCAGCAAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hck Promotes Neuronal Apoptosis Following Intracerebral Hemorrhage.
Cellular and molecular neurobiology37(2)251-261(2017 Mar)
Wang J,Chen R,Liu X,Shen J,Yan Y,Gao Y,Tao T,Shi J

Tarp regulates early Chlamydia-induced host cell survival through interactions with the human adaptor protein SHC1.
The Journal of cell biology190(1)143-57(2010 Jul)
Mehlitz A,Banhart S,M?urer AP,Kaushansky A,Gordus AG,Zielecki J,Macbeath G,Meyer TF

Regulation of p73 by Hck through kinase-dependent and independent mechanisms.
BMC molecular biology845(2007 May)
Paliwal P,Radha V,Swarup G

Differential kinase requirements in human and mouse Fc-gamma receptor phagocytosis and endocytosis.
Journal of leukocyte biology80(6)1553-62(2006 Dec)
Huang ZY,Barreda DR,Worth RG,Indik ZK,Kim MK,Chien P,Schreiber AD

Activation of the lysosome-associated p61Hck isoform triggers the biogenesis of podosomes.
Traffic (Copenhagen, Denmark)6(8)682-94(2005 Aug)
Cougoule C,Carr?no S,Castandet J,Labrousse A,Astarie-Dequeker C,Poincloux R,Le Cabec V,Maridonneau-Parini I

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Hck cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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