Selenow cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Selenow
Entrez Gene ID	25545
Full Name	selenoprotein W
Synonyms	SelW,Sepw1
General protein information	Preferred Names selenoprotein W Names selenoprotein W selenoprotein W, 1 selenoprotein W, muscle 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is highly expressed in skeletal muscle and brain. It belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Studies in mouse show that this selenoprotein is involved in muscle growth and differentiation, and in the protection of neurons from oxidative stress during neuronal development. A pseudogene of this locus has been identified on chromosome 10. [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013027.3	NP_037159.4	selenoprotein W

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sepw1	NP_037159.4
Pan troglodytes (chimpanzee)	SEPW1	NP_001148049.1
Gallus gallus (chicken)	SEPW1	NP_001159799.1
Danio rerio (zebrafish)	sepw1	NP_840072.3
Homo sapiens (human)	SEPW1	NP_003000.1
Bos taurus (cattle)	SEPW1	NP_001156697.1
	SEPW1	NP_001036202.1
Canis lupus familiaris (dog)	SEPW1	NP_001108484.1
Mus musculus (house mouse)	Sepw1	NP_033182.1

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Transcriptional regulation of selenoprotein W by MyoD during early skeletal muscle differentiation.
Noh OJ, Park YH, Chung YW, Kim IY
The Journal of biological chemistry285(52)40496-507(2010 Dec)

Selenium requirements are higher for glutathione peroxidase-1 mRNA than gpx1 activity in rat testis.
Schriever SC, Barnes KM, Evenson JK, Raines AM, Sunde RA
Experimental biology and medicine (Maywood, N.J.)234(5)513-21(2009 May)

Identification of putative transcription factor binding sites in rodent selenoprotein W promoter.
Amantana A, Vorachek WR, Butler JA, Ream W, Whanger PD
Journal of inorganic biochemistry98(9)1513-20(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SelW gene was activated by the binding of MyoD to a specific E-box during early skeletal muscle differentiation.
Title: Transcriptional regulation of selenoprotein W by MyoD during early skeletal muscle differentiation.

the SeW gene may be a target for Sp1 whose binding to various regulatory sequences of the SeW promoter may activate or repress the transcription of SeW
Title: Identification of putative transcription factor binding sites in rodent selenoprotein W promoter.

Se-W plays an important role as an antioxidant in the developing brain and embryo
Title: Different distributions of selenoprotein W and thioredoxin during postnatal brain development and embryogenesis.

The following Selenow gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Selenow cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12118	NM_013027.3 Latest version!	Rattus norvegicus selenoprotein W (Selenow), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Selenow ( NM_013027.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037159.4 Rattus norvegicus selenoprotein W (Selenow), mRNA.

Price & Availability↑

CloneID	ORa12118
Clone ID Related Accession (Same CDS sequence)	NM_013027.3
Accession Version	NM_013027.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	selenoprotein W
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI279050.1, BC087625.1 and BI281207.1. On Dec 20, 2008 this sequence version replaced NM_013027.2. ##Evidence-Data-START## Transcript exon combination :: FQ224556.1, FQ217426.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMN01906348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:8349599) protein contains selenocysteine :: PMID: 7568010 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTAG CCGTTCGAGT CGTGTATTGT GGAGCTTGAG GCTATAAGCC CAAGTATCTC 
CAGCTCAAGG AGAAGCTAGA ACATGAGTTC CCCGGATGCC TGGACATCTG TGGCGAGGGG 
ACTCCCCAGG TCACCGGGTT CTTTGAAGTG ACGGTAGCCG GGAAGTTGGT TCACTCCAAG 
AAGAGAGGTG ATGGCTACGT GGATACAGAG AGCAAGTTCC GGAAACTGGT GACTGCCATC 
AAAGCCGCCT TGGCTCAGTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037159.4
CDS	85..351
Translation	MALAVRVVYCGA*GYKPKYLQLKEKLEHEFPGCLDICGEGTPQVTGFFEVTVAGKLVHSKKRGDGYVDTESKFRKLVTAIKAALAQCQ

Target ORF information:

RefSeq Version	NM_013027.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus selenoprotein W (Selenow), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013027.3

ORF Insert Sequence:

ATGGCGCTAG CCGTTCGAGT CGTGTATTGT GGAGCTTGAG GCTATAAGCC CAAGTATCTC 
CAGCTCAAGG AGAAGCTAGA ACATGAGTTC CCCGGATGCC TGGACATCTG TGGCGAGGGG 
ACTCCCCAGG TCACCGGGTT CTTTGAAGTG ACGGTAGCCG GGAAGTTGGT TCACTCCAAG 
AAGAGAGGTG ATGGCTACGT GGATACAGAG AGCAAGTTCC GGAAACTGGT GACTGCCATC 
AAAGCCGCCT TGGCTCAGTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Transcriptional regulation of selenoprotein W by MyoD during early skeletal muscle differentiation.
The Journal of biological chemistry285(52)40496-507(2010 Dec)
Noh OJ,Park YH,Chung YW,Kim IY

Selenium requirements are higher for glutathione peroxidase-1 mRNA than gpx1 activity in rat testis.
Experimental biology and medicine (Maywood, N.J.)234(5)513-21(2009 May)
Schriever SC,Barnes KM,Evenson JK,Raines AM,Sunde RA

Identification of putative transcription factor binding sites in rodent selenoprotein W promoter.
Journal of inorganic biochemistry98(9)1513-20(2004 Sep)
Amantana A,Vorachek WR,Butler JA,Ream W,Whanger PD

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Selenow cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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