SEPW1 cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEPW1
Entrez Gene ID	6415
Full Name	selenoprotein W
Synonyms	SEPW1,selW
General protein information	Preferred Names selenoprotein W Names selenoprotein W selenoprotein W, 1
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 19 Map Location: 19q13.33
Summary	This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is highly expressed in skeletal muscle, heart and brain. It belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Studies in mouse show that this selenoprotein is involved in muscle growth and differentiation, and in the protection of neurons from oxidative stress during neuronal development. A retroprocessed pseudogene of this locus has been identified on chromosome 1. [provided by RefSeq, Aug 2017].
Disorder MIM:	603235

mRNA and Protein(s)

mRNA	Protein	Name
NM_003009.3	NP_003000.1	selenoprotein W
NM_003009.4	NP_003000.1	selenoprotein W
NM_003009.2	NP_003000.1	selenoprotein W

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sepw1	NP_037159.4
Pan troglodytes (chimpanzee)	SEPW1	NP_001148049.1
Gallus gallus (chicken)	SEPW1	NP_001159799.1
Danio rerio (zebrafish)	sepw1	NP_840072.3
Homo sapiens (human)	SEPW1	NP_003000.1
Bos taurus (cattle)	SEPW1	NP_001156697.1
	SEPW1	NP_001036202.1
Canis lupus familiaris (dog)	SEPW1	NP_001108484.1
Mus musculus (house mouse)	Sepw1	NP_033182.1

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Identification of a redox-modulatory interaction between selenoprotein W and 14-3-3 protein.
Jeon YH, Ko KY, Lee JH, Park KJ, Jang JK, Kim IY
Biochimica et biophysica acta1863(1)10-8(2016 Jan)

Selenoprotein W controls epidermal growth factor receptor surface expression, activation and degradation via receptor ubiquitination.
Alkan Z, Duong FL, Hawkes WC
Biochimica et biophysica acta1853(5)1087-95(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SelW may have a regulatory function in redox cell signaling by interacting with 14-3-3 protein.
Title: Identification of a redox-modulatory interaction between selenoprotein W and 14-3-3 protein.

Suppression of EGFR ubiquitination by SEPW1 may be related to the putative increase in cancer risk associated with high selenium intakes.
Title: Selenoprotein W controls epidermal growth factor receptor surface expression, activation and degradation via receptor ubiquitination.

In response to selenium compounds, SepW1 synthesis increased at the protein and the mRNA levels.
Title: Selenoprotein W as biomarker for the efficacy of selenium compounds to act as source for selenoprotein biosynthesis.

SEPW1 silencing increases MKK4, which activates p38gamma, p38delta, and JNK2 to phosphorylate p53 on Ser-33 and cause a transient G(1) arrest.
Title: Delayed cell cycle progression in selenoprotein W-depleted cells is regulated by a mitogen-activated protein kinase kinase 4-p38/c-Jun NH2-terminal kinase-p53 pathway.

The present work shows that SEPW1 facilitates the G1 to S-phase transition by down-regulating expression of the cyclin-dependent kinase inhibitor p21
Title: Delayed cell cycle progression from SEPW1 depletion is p53- and p21-dependent in MCF-7 breast cancer cells.

The following SEPW1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEPW1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31633	NM_003009.4 Latest version!	Homo sapiens selenoprotein W (SELENOW), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu31633	NM_003009.3	Homo sapiens selenoprotein W (SELENOW), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31633	NM_003009.2	Homo sapiens selenoprotein W, 1 (SEPW1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEPW1 ( NM_003009.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_003000.1 Homo sapiens selenoprotein W (SELENOW), mRNA.

Price & Availability↑

CloneID	OHu31633
Clone ID Related Accession (Same CDS sequence)	NM_003009.3 , NM_003009.4 , NM_003009.2
Accession Version	NM_003009.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	selenoprotein W
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF973456.1, CR991097.1 and BC039597.1. On Apr 28, 2017 this sequence version replaced NM_003009.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279849.780.1, ERR279829.452.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:9256076) protein contains selenocysteine :: PMID: 12818432 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTCG CCGTCCGAGT CGTTTATTGT GGCGCTTGAG GCTACAAGTC CAAGTATCTT 
CAGCTCAAGA AGAAGTTAGA AGATGAGTTC CCCGGCCGCC TGGACATCTG CGGCGAGGGA 
ACTCCCCAGG CCACCGGGTT CTTTGAAGTG ATGGTAGCCG GGAAGTTGAT TCACTCTAAG 
AAGAAAGGCG ATGGCTACGT GGACACAGAA AGCAAGTTTC TGAAGTTGGT GGCCGCCATC 
AAAGCCGCCT TGGCTCAGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003000.1
CDS	215..478
Translation	MALAVRVVYCGA*GYKSKYLQLKKKLEDEFPGRLDICGEGTPQATGFFEVMVAGKLIHSKKKGDGYVDTESKFLKLVAAIKAALAQG

Target ORF information:

RefSeq Version	NM_003009.3
Organism	Homo sapiens(human)
Definition	Homo sapiens selenoprotein W (SELENOW), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003009.3

ORF Insert Sequence:

ATGGCTCTCG CCGTCCGAGT CGTTTATTGT GGCGCTTGAG GCTACAAGTC CAAGTATCTT 
CAGCTCAAGA AGAAGTTAGA AGATGAGTTC CCCGGCCGCC TGGACATCTG CGGCGAGGGA 
ACTCCCCAGG CCACCGGGTT CTTTGAAGTG ATGGTAGCCG GGAAGTTGAT TCACTCTAAG 
AAGAAAGGCG ATGGCTACGT GGACACAGAA AGCAAGTTTC TGAAGTTGGT GGCCGCCATC 
AAAGCCGCCT TGGCTCAGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEPW1 ( NM_003009.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_003000.1 Homo sapiens selenoprotein W (SELENOW), mRNA.

Price & Availability↑

CloneID	OHu31633
Clone ID Related Accession (Same CDS sequence)	NM_003009.3 , NM_003009.4 , NM_003009.2
Accession Version	NM_003009.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	selenoprotein W
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR991097.1, BC039597.1 and AC008745.7. On Nov 23, 2018 this sequence version replaced NM_003009.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189652.162141.1, SRR5189652.186262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:9256076) MANE Ensembl match :: ENST00000601048.6/ ENSP00000473185.1 protein contains selenocysteine :: PMID: 12818432 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCTCG CCGTCCGAGT CGTTTATTGT GGCGCTTGAG GCTACAAGTC CAAGTATCTT 
CAGCTCAAGA AGAAGTTAGA AGATGAGTTC CCCGGCCGCC TGGACATCTG CGGCGAGGGA 
ACTCCCCAGG CCACCGGGTT CTTTGAAGTG ATGGTAGCCG GGAAGTTGAT TCACTCTAAG 
AAGAAAGGCG ATGGCTACGT GGACACAGAA AGCAAGTTTC TGAAGTTGGT GGCCGCCATC 
AAAGCCGCCT TGGCTCAGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003000.1
CDS	84..347
Translation	MALAVRVVYCGA*GYKSKYLQLKKKLEDEFPGRLDICGEGTPQATGFFEVMVAGKLIHSKKKGDGYVDTESKFLKLVAAIKAALAQG

Target ORF information:

RefSeq Version	NM_003009.4
Organism	Homo sapiens(human)
Definition	Homo sapiens selenoprotein W (SELENOW), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003009.4

ORF Insert Sequence:

ATGGCTCTCG CCGTCCGAGT CGTTTATTGT GGCGCTTGAG GCTACAAGTC CAAGTATCTT 
CAGCTCAAGA AGAAGTTAGA AGATGAGTTC CCCGGCCGCC TGGACATCTG CGGCGAGGGA 
ACTCCCCAGG CCACCGGGTT CTTTGAAGTG ATGGTAGCCG GGAAGTTGAT TCACTCTAAG 
AAGAAAGGCG ATGGCTACGT GGACACAGAA AGCAAGTTTC TGAAGTTGGT GGCCGCCATC 
AAAGCCGCCT TGGCTCAGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEPW1 ( NM_003009.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_003000.1 Homo sapiens selenoprotein W, 1 (SEPW1), mRNA.

Price & Availability↑

CloneID	OHu31633
Clone ID Related Accession (Same CDS sequence)	NM_003009.3 , NM_003009.4 , NM_003009.2
Accession Version	NM_003009.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-09
Organism	Homo sapiens(Human)
Product	selenoprotein W
Comment

ATGGCTCTCG CCGTCCGAGT CGTTTATTGT GGCGCTTGAG GCTACAAGTC CAAGTATCTT 
CAGCTCAAGA AGAAGTTAGA AGATGAGTTC CCCGGCCGCC TGGACATCTG CGGCGAGGGA 
ACTCCCCAGG CCACCGGGTT CTTTGAAGTG ATGGTAGCCG GGAAGTTGAT TCACTCTAAG 
AAGAAAGGCG ATGGCTACGT GGACACAGAA AGCAAGTTTC TGAAGTTGGT GGCCGCCATC 
AAAGCCGCCT TGGCTCAGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003000.1
Translation	MALAVRVVYCGA*GYKSKYLQLKKKLEDEFPGRLDICGEGTPQATGFFEVMVAGKLIHSKKKGDGYVDTESKFLKLVAAIKAALAQG

Target ORF information:

RefSeq Version	NM_003009.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens selenoprotein W, 1 (SEPW1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003009.2

ORF Insert Sequence:

ATGGCTCTCG CCGTCCGAGT CGTTTATTGT GGCGCTTGAG GCTACAAGTC CAAGTATCTT 
CAGCTCAAGA AGAAGTTAGA AGATGAGTTC CCCGGCCGCC TGGACATCTG CGGCGAGGGA 
ACTCCCCAGG CCACCGGGTT CTTTGAAGTG ATGGTAGCCG GGAAGTTGAT TCACTCTAAG 
AAGAAAGGCG ATGGCTACGT GGACACAGAA AGCAAGTTTC TGAAGTTGGT GGCCGCCATC 
AAAGCCGCCT TGGCTCAGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Identification of a redox-modulatory interaction between selenoprotein W and 14-3-3 protein.
Biochimica et biophysica acta1863(1)10-8(2016 Jan)
Jeon YH,Ko KY,Lee JH,Park KJ,Jang JK,Kim IY

Selenoprotein W controls epidermal growth factor receptor surface expression, activation and degradation via receptor ubiquitination.
Biochimica et biophysica acta1853(5)1087-95(2015 May)
Alkan Z,Duong FL,Hawkes WC

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SEPW1 cDNA ORF clone, Homo sapiens(Human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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