Ccl3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ccl3
Entrez Gene ID	25542
Full Name	C-C motif chemokine ligand 3
Synonyms	MIP-1a,Scya3
General protein information	Preferred Names C-C motif chemokine ligand 3 Names C-C motif chemokine 3 MIP-1-alpha Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3) chemokine (C-C motif) ligand 3 macrophage inflammatory protein 1-alpha small-inducible cytokine A3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26
Summary	mediates monocyte and neutrophil chemotaxis; may play a role in the pathogenesis of acute lung injury [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013025.2	NP_037157.2	C-C motif chemokine 3 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC749948	XP_001172388.1
Pan troglodytes (chimpanzee)	LOC749913	XP_001172226.1
Canis lupus familiaris (dog)	CCL3	NP_001005251.1
Bos taurus (cattle)	CCL3	NP_776936.1
Homo sapiens (human)	CCL3L3	NP_001001437.2
	LOC100423954	XP_002800705.1
Rattus norvegicus (Norway rat)	Ccl3	NP_037157.2
Mus musculus (house mouse)	Ccl3	NP_035467.1

Gene Ontology
Bibliography

Related articles in PubMed

JNK signaling is required for the MIP?1??associated regulation of Kupffer cells in the heat stroke response.
Chen XJ, Tang ZZ, Zhu GG, Cheng Q, Zhang WK, Li HM, Fu W, Lu QP
Molecular medicine reports16(3)2389-2396(2017 Sep)

Involvement of CCL3/CCR5 Signaling in Dorsal Root Ganglion in Remifentanil-induced Hyperalgesia in Rats.
Li N, Zhang L, Shu R, Ding L, Wang Z, Wang H, Yu Y, Wang G
The Clinical journal of pain32(8)702-10(2016 Aug)

Erythropoietin and CCL3 antagonise their functional properties during neuroinflammation.
Hattermann K, Knerlich-Lukoschus F, Lucius R, Mehdorn M, Held-Feindt J
Neurological research37(11)1025-8(2015 Nov)

Involvement of the chemokine CCL3 and the purinoceptor P2X7 in the spinal cord in paclitaxel-induced mechanical allodynia.
Ochi-ishi R, Nagata K, Inoue T, Tozaki-Saitoh H, Tsuda M, Inoue K
Molecular pain1053(2014 Aug)

Chemokine (C-C motif) receptor 5 is an important pathological regulator in the development and maintenance of neuropathic pain.
Matsushita K, Tozaki-Saitoh H, Kojima C, Masuda T, Tsuda M, Inoue K, Hoka S
Anesthesiology120(6)1491-503(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The present report demonstrates an increase in mRNA and protein levels in chemokines CCL3 and its receptor CCR5 in dorsal root ganglion in the rat model of remifentanil-induced hyperalgesia. Furthermore, the results pointed out that CCL3 and its receptor CCR5 might be implicated in remifentanil-induced hyperalgesia.
Title: Involvement of CCL3/CCR5 Signaling in Dorsal Root Ganglion in Remifentanil-induced Hyperalgesia in Rats.

Study shows that erythropoietin reduced chemokine CCL3 mRNA expression in rat cerebellar neuron-enriched preparations during neuroinflammation.
Title: Erythropoietin and CCL3 antagonise their functional properties during neuroinflammation.

The results of this study suggest a contribution of CCL3 in the spinal dorsal horn to paclitaxel-induced allodynia and may provide new therapeutic targets for paclitaxel-induced painful neuropathy.
Title: Involvement of the chemokine CCL3 and the purinoceptor P2X7 in the spinal cord in paclitaxel-induced mechanical allodynia.

Expression levels of CCL3 in the spinal cord are up-regulated following nerve injury.
Title: Chemokine (C-C motif) receptor 5 is an important pathological regulator in the development and maintenance of neuropathic pain.

NMDA-evoked neuronal injury differentially induces MIP-1alpha and MCP-1 production in microglia and astrocytes, respectively, through activation of different intracellular signaling pathways.
Title: Neuronal injury induces microglial production of macrophage inflammatory protein-1? in rat corticostriatal slice cultures.

The following Ccl3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ccl3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01683	NM_013025.2 Latest version!	Rattus norvegicus C-C motif chemokine ligand 3 (Ccl3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ccl3 ( NM_013025.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037157.2 Rattus norvegicus C-C motif chemokine ligand 3 (Ccl3), mRNA.

Price & Availability↑

CloneID	ORa01683
Clone ID Related Accession (Same CDS sequence)	NM_013025.2
Accession Version	NM_013025.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	C-C motif chemokine 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U22414.1. On Dec 20, 2003 this sequence version replaced NM_013025.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U22414.1, DV728694.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689594 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTCT CCACCGCTGC CCTTGCTGTT CTTCTCTGCA CCATGGCGCT CTGGAACGAA 
GTCTTCTCAG CGCCATATGG AGCTGACACC CCGACTGCCT GCTGCTTCTC CTATGGACGG 
CAAATTCCAC GAAAATTCAT TGCTGACTAT TTTGAGACCA GCAGCCTTTG CTCCCAGCCG 
GGTGTCATTT TCCTGACCAA GAGAAACCGG CAGATCTGCG CTGACCCCAA AGAGACCTGG 
GTCCAAGAAT ACATCACTGA GCTGGAACTA AATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037157.2
CDS	70..348
Translation	MKVSTAALAVLLCTMALWNEVFSAPYGADTPTACCFSYGRQIPRKFIADYFETSSLCSQPGVIFLTKRNRQICADPKETWVQEYITELELNA

Target ORF information:

RefSeq Version	NM_013025.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus C-C motif chemokine ligand 3 (Ccl3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013025.2

ORF Insert Sequence:

ATGAAGGTCT CCACCGCTGC CCTTGCTGTT CTTCTCTGCA CCATGGCGCT CTGGAACGAA 
GTCTTCTCAG CGCCATATGG AGCTGACACC CCGACTGCCT GCTGCTTCTC CTATGGACGG 
CAAATTCCAC GAAAATTCAT TGCTGACTAT TTTGAGACCA GCAGCCTTTG CTCCCAGCCG 
GGTGTCATTT TCCTGACCAA GAGAAACCGG CAGATCTGCG CTGACCCCAA AGAGACCTGG 
GTCCAAGAAT ACATCACTGA GCTGGAACTA AATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

JNK signaling is required for the MIP?1??associated regulation of Kupffer cells in the heat stroke response.
Molecular medicine reports16(3)2389-2396(2017 Sep)
Chen XJ,Tang ZZ,Zhu GG,Cheng Q,Zhang WK,Li HM,Fu W,Lu QP

Involvement of CCL3/CCR5 Signaling in Dorsal Root Ganglion in Remifentanil-induced Hyperalgesia in Rats.
The Clinical journal of pain32(8)702-10(2016 Aug)
Li N,Zhang L,Shu R,Ding L,Wang Z,Wang H,Yu Y,Wang G

Erythropoietin and CCL3 antagonise their functional properties during neuroinflammation.
Neurological research37(11)1025-8(2015 Nov)
Hattermann K,Knerlich-Lukoschus F,Lucius R,Mehdorn M,Held-Feindt J

Involvement of the chemokine CCL3 and the purinoceptor P2X7 in the spinal cord in paclitaxel-induced mechanical allodynia.
Molecular pain1053(2014 Aug)
Ochi-ishi R,Nagata K,Inoue T,Tozaki-Saitoh H,Tsuda M,Inoue K

Chemokine (C-C motif) receptor 5 is an important pathological regulator in the development and maintenance of neuropathic pain.
Anesthesiology120(6)1491-503(2014 Jun)
Matsushita K,Tozaki-Saitoh H,Kojima C,Masuda T,Tsuda M,Inoue K,Hoka S

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Ccl3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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