Npm1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npm1
Entrez Gene ID	25498
Full Name	nucleophosmin 1
Synonyms	B23NP
General protein information	Preferred Names nucleophosmin 1 Names nucleophosmin NPM nucleolar phosphoprotein B23 nucleolar protein B23.1 nucleolar protein NO38 nucleophosmin (nucleolar phosphoprotein B23, numatrin) numatrin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12
Summary	RNA-associated nucleolar phosphoprotein; involved in ribosome assembly [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008767546.2	XP_008765768.1	nucleophosmin isoform X1
NM_012992.4	NP_037124.1	nucleophosmin

Pathways from BioSystems

Homologies

	LOC706883	XP_002804677.1
Bos taurus (cattle)	NPM1	NP_001030518.1
Rattus norvegicus (Norway rat)	Npm1	NP_037124.1
Gallus gallus (chicken)	NPM1	NP_990598.1
Mus musculus (house mouse)	Npm1	NP_032748.1
Danio rerio (zebrafish)	npm1a	NP_955460.1
Homo sapiens (human)	NPM1	NP_002511.1
Pan troglodytes (chimpanzee)	NPM1	NP_001238905.1
Xenopus tropicalis (tropical clawed frog)	npm1	NP_988883.1
Canis lupus familiaris (dog)	NPM1	NP_001239100.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of Neuronal Survival by Nucleophosmin 1 (NPM1) Is Dependent on Its Expression Level, Subcellular Localization, and Oligomerization Status.
Pfister JA, D'Mello SR
The Journal of biological chemistry291(39)20787-97(2016 09)

The Nucleolar Protein GLTSCR2 Is an Upstream Negative Regulator of the Oncogenic Nucleophosmin-MYC Axis.
Kim JY, Cho YE, Park JH
The American journal of pathology185(7)2061-8(2015 Jul)

Altered HLA Class I Profile Associated with Type A/D Nucleophosmin Mutation Points to Possible Anti-Nucleophosmin Immune Response in Acute Myeloid Leukemia.
Ku?elov? K, Brodsk? B, Fuchs O, Dobrovoln? M, Soukup P, Cetkovsk? P
PloS one10(5)e0127637(2015)

Synaptotagmin 11 interacts with components of the RNA-induced silencing complex RISC in clonal pancreatic ?-cells.
Milochau A, Lagr?e V, Benassy MN, Chaignepain S, Papin J, Garcia-Arcos I, Lajoix A, Monterrat C, Coudert L, Schmitter JM, Ochoa B, Lang J
FEBS letters588(14)2217-22(2014 Jun)

Structural polymorphism in the N-terminal oligomerization domain of NPM1.
Mitrea DM, Grace CR, Buljan M, Yun MK, Pytel NJ, Satumba J, Nourse A, Park CG, Madan Babu M, White SW, Kriwacki RW
Proceedings of the National Academy of Sciences of the United States of America111(12)4466-71(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study suggests that although neurons need NPM1 for survival, an increase in its expression beyond physiological levels and its translocation to the cytoplasm leads to death through abortive cell cycle induction.
Title: Regulation of Neuronal Survival by Nucleophosmin 1 (NPM1) Is Dependent on Its Expression Level, Subcellular Localization, and Oligomerization Status.

The S-nitrosylation of B23 enhanced B23-SIAH1 binding and mediated the neuroprotective actions of B23 by abrogating the E3 ligase activity of SIAH1.
Title: S-nitrosylation of B23/nucleophosmin by GAPDH protects cells from the SIAH1-GAPDH death cascade.

These results suggest a key role for NPM in promoting neuronal survival and a novel mechanism of neuronal degeneration triggered by nucleolar stress.
Title: Nucleolar stress characterized by downregulation of nucleophosmin: a novel cause of neuronal degeneration.

Nuclear localization of B23 and CK2 is dynamically regulated in response to altered growth status in the cell.
Title: Protein B23/nucleophosmin/numatrin nuclear dynamics in relation to protein kinase CK2 and apoptotic activity in prostate cells.

Npm1 may act as an alarmin: implications for sepsis are reported.
Title: Nucleophosmin may act as an alarmin: implications for severe sepsis.

The following Npm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01575	NM_012992.4 Latest version!	Rattus norvegicus nucleophosmin 1 (Npm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
ORa43126	XM_008767546.2 Latest version!	Rattus norvegicus nucleophosmin 1 (Npm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npm1 ( NM_012992.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037124.1 Rattus norvegicus nucleophosmin 1 (Npm1), mRNA.

Price & Availability↑

CloneID	ORa01575
Clone ID Related Accession (Same CDS sequence)	NM_012992.4
Accession Version	NM_012992.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	nucleophosmin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC060579.1. On Aug 30, 2012 this sequence version replaced NM_012992.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060579.1, J03969.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01906347, SAMN01906349 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGACT CGATGGACAT GGACATGAGC CCTCTTAGGC CTCAGAACTA CCTTTTCGGT 
TGTGAACTAA AGGCTGACAA AGATTATCAC TTTAAAGTGG ATAATGATGA AAATGAGCAC 
CAGTTATCAT TAAGAACGGT CAGTTTAGGA GCAGGGGCAA AAGATGAGTT GCACATCGTA 
GAGGCAGAAG CAATGAACTA TGAAGGCAGC CCAATTAAAG TAACACTGGC AACTTTGAAA 
ATGTCTGTAC AACCAACAGT TTCCCTTGGG GGCTTCGAAA TTACACCACC TGTGGTCTTG 
AGGTTGAAGT GTGGTTCTGG GCCTGTGCAC ATAAGTGGAC AGCACCTAGT AGCTGTAGAG 
GAAGATGCAG AGTCAGAAGA TGAAGATGAG GAAGATGTAA AACTCTTAGG CATGTCTGGA 
AAGAGATCTG CTCCCGGAGG TGGTAACAAA GTCCCACAGA AAAAAGTAAA ACTTGATGAA 
GATGATGATG AGGATGATGA AGATGATGAG GATGATGAAG ATGATGATGA TGATGATTTT 
GATGAAGAGG AAACTGAAGA AAAGGTTCCA GTGAAGAAAT CTGTACGAGA TACCCCAGCC 
AAAAATGCAC AAAAATCAAA CCAAAATGGG AAAGATTTAA AACCATCAAC ACCAAGGTCA 
AAGGGTCAAG AGTCCTTCAA AAAACAGGAA AAAACTCCCA AAACACCCAA AGGACCTAGC 
TCTGTAGAAG ACATTAAGGC AAAAATGCAA GCAAGTATAG AAAAAGGTGG TTCTCTTCCC 
AAAGTGGAAG CCAAGTTCAT TAATTATGTG AAGAATTGTT TCCGGATGAC TGACCAGGAG 
GCTATTCAAG ATCTCTGGCA GTGGAGGAAG TCTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037124.1
CDS	94..972
Translation	MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEDEEDVKLLGMSGKRSAPGGGNKVPQKKVKLDEDDDEDDEDDEDDEDDDDDDFDEEETEEKVPVKKSVRDTPAKNAQKSNQNGKDLKPSTPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL

Target ORF information:

RefSeq Version	NM_012992.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nucleophosmin 1 (Npm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012992.4

ORF Insert Sequence:

ATGGAAGACT CGATGGACAT GGACATGAGC CCTCTTAGGC CTCAGAACTA CCTTTTCGGT 
TGTGAACTAA AGGCTGACAA AGATTATCAC TTTAAAGTGG ATAATGATGA AAATGAGCAC 
CAGTTATCAT TAAGAACGGT CAGTTTAGGA GCAGGGGCAA AAGATGAGTT GCACATCGTA 
GAGGCAGAAG CAATGAACTA TGAAGGCAGC CCAATTAAAG TAACACTGGC AACTTTGAAA 
ATGTCTGTAC AACCAACAGT TTCCCTTGGG GGCTTCGAAA TTACACCACC TGTGGTCTTG 
AGGTTGAAGT GTGGTTCTGG GCCTGTGCAC ATAAGTGGAC AGCACCTAGT AGCTGTAGAG 
GAAGATGCAG AGTCAGAAGA TGAAGATGAG GAAGATGTAA AACTCTTAGG CATGTCTGGA 
AAGAGATCTG CTCCCGGAGG TGGTAACAAA GTCCCACAGA AAAAAGTAAA ACTTGATGAA 
GATGATGATG AGGATGATGA AGATGATGAG GATGATGAAG ATGATGATGA TGATGATTTT 
GATGAAGAGG AAACTGAAGA AAAGGTTCCA GTGAAGAAAT CTGTACGAGA TACCCCAGCC 
AAAAATGCAC AAAAATCAAA CCAAAATGGG AAAGATTTAA AACCATCAAC ACCAAGGTCA 
AAGGGTCAAG AGTCCTTCAA AAAACAGGAA AAAACTCCCA AAACACCCAA AGGACCTAGC 
TCTGTAGAAG ACATTAAGGC AAAAATGCAA GCAAGTATAG AAAAAGGTGG TTCTCTTCCC 
AAAGTGGAAG CCAAGTTCAT TAATTATGTG AAGAATTGTT TCCGGATGAC TGACCAGGAG 
GCTATTCAAG ATCTCTGGCA GTGGAGGAAG TCTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npm1 ( XM_008767546.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765768.1 Rattus norvegicus nucleophosmin 1 (Npm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43126
Clone ID Related Accession (Same CDS sequence)	XM_008767546.2
Accession Version	XM_008767546.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nucleophosmin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGCGG AGGCGGGACT AGAATGGATC TCGCGAGATC TCCTGCGGCC ATATATACGT 
GCGGCGAGCC CGCGTCCTTT CCTTGGCGTG ATTCCGTCCT GCGTGTCTGT TCTGCGGAAC 
AGTAGGCAGT TGTTTTCCGT CCGGCTTCTC TCACACTCAA GTGCGCGCCT CCACCTCATG 
GAAGACTCGA TGGACATGGA CATGAGCCCT CTTAGGCCTC AGAACTACCT TTTCGGTTGT 
GAACTAAAGG CTGACAAAGA TTATCACTTT AAAGTGGATA ATGATGAAAA TGAGCACCAG 
TTATCATTAA GAACGGTCAG TTTAGGAGCA GGGGCAAAAG ATGAGTTGCA CATCGTAGAG 
GCAGAAGCAA TGAACTATGA AGGCAGCCCA ATTAAAGTAA CACTGGCAAC TTTGAAAATG 
TCTGTACAAC CAACAGTTTC CCTTGGGGGC TTCGAAATTA CACCACCTGT GGTCTTGAGG 
TTGAAGTGTG GTTCTGGGCC TGTGCACATA AGTGGACAGC ACCTAGTAGC TGTAGAGGAA 
GATGCAGAGT CAGAAGATGA AGATGAGGAA GATGTAAAAC TCTTAGGCAT GTCTGGAAAG 
AGATCTGCTC CCGGAGGTGG TAACAAAGTC CCACAGAAAA AAGTAAAACT TGATGAAGAT 
GATGATGAGG ATGATGAAGA TGATGAGGAT GATGAAGATG ATGATGATGA TGATTTTGAT 
GAAGAGGAAA CTGAAGAAAA GGTTCCAGTG AAGAAATCTG TACGAGATAC CCCAGCCAAA 
AATGCACAAA AATCAAACCA AAATGGGAAA GATTTAAAAC CATCAACACC AAGGTCAAAG 
GGTCAAGAGT CCTTCAAAAA ACAGGAAAAA ACTCCCAAAA CACCCAAAGG ACCTAGCTCT 
GTAGAAGACA TTAAGGCAAA AATGCAAGCA AGTATAGAAA AAGCGCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765768.1
CDS	29..979
Translation	MLAEAGLEWISRDLLRPYIRAASPRPFLGVIPSCVSVLRNSRQLFSVRLLSHSSARLHLMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEDEEDVKLLGMSGKRSAPGGGNKVPQKKVKLDEDDDEDDEDDEDDEDDDDDDFDEEETEEKVPVKKSVRDTPAKNAQKSNQNGKDLKPSTPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKAH

Target ORF information:

RefSeq Version	XM_008767546.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nucleophosmin 1 (Npm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767546.2

ORF Insert Sequence:

ATGCTCGCGG AGGCGGGACT AGAATGGATC TCGCGAGATC TCCTGCGGCC ATATATACGT 
GCGGCGAGCC CGCGTCCTTT CCTTGGCGTG ATTCCGTCCT GCGTGTCTGT TCTGCGGAAC 
AGTAGGCAGT TGTTTTCCGT CCGGCTTCTC TCACACTCAA GTGCGCGCCT CCACCTCATG 
GAAGACTCGA TGGACATGGA CATGAGCCCT CTTAGGCCTC AGAACTACCT TTTCGGTTGT 
GAACTAAAGG CTGACAAAGA TTATCACTTT AAAGTGGATA ATGATGAAAA TGAGCACCAG 
TTATCATTAA GAACGGTCAG TTTAGGAGCA GGGGCAAAAG ATGAGTTGCA CATCGTAGAG 
GCAGAAGCAA TGAACTATGA AGGCAGCCCA ATTAAAGTAA CACTGGCAAC TTTGAAAATG 
TCTGTACAAC CAACAGTTTC CCTTGGGGGC TTCGAAATTA CACCACCTGT GGTCTTGAGG 
TTGAAGTGTG GTTCTGGGCC TGTGCACATA AGTGGACAGC ACCTAGTAGC TGTAGAGGAA 
GATGCAGAGT CAGAAGATGA AGATGAGGAA GATGTAAAAC TCTTAGGCAT GTCTGGAAAG 
AGATCTGCTC CCGGAGGTGG TAACAAAGTC CCACAGAAAA AAGTAAAACT TGATGAAGAT 
GATGATGAGG ATGATGAAGA TGATGAGGAT GATGAAGATG ATGATGATGA TGATTTTGAT 
GAAGAGGAAA CTGAAGAAAA GGTTCCAGTG AAGAAATCTG TACGAGATAC CCCAGCCAAA 
AATGCACAAA AATCAAACCA AAATGGGAAA GATTTAAAAC CATCAACACC AAGGTCAAAG 
GGTCAAGAGT CCTTCAAAAA ACAGGAAAAA ACTCCCAAAA CACCCAAAGG ACCTAGCTCT 
GTAGAAGACA TTAAGGCAAA AATGCAAGCA AGTATAGAAA AAGCGCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of Neuronal Survival by Nucleophosmin 1 (NPM1) Is Dependent on Its Expression Level, Subcellular Localization, and Oligomerization Status.
The Journal of biological chemistry291(39)20787-97(2016 09)
Pfister JA,D'Mello SR

The Nucleolar Protein GLTSCR2 Is an Upstream Negative Regulator of the Oncogenic Nucleophosmin-MYC Axis.
The American journal of pathology185(7)2061-8(2015 Jul)
Kim JY,Cho YE,Park JH

Altered HLA Class I Profile Associated with Type A/D Nucleophosmin Mutation Points to Possible Anti-Nucleophosmin Immune Response in Acute Myeloid Leukemia.
PloS one10(5)e0127637(2015)
Ku?elov? K,Brodsk? B,Fuchs O,Dobrovoln? M,Soukup P,Cetkovsk? P

Synaptotagmin 11 interacts with components of the RNA-induced silencing complex RISC in clonal pancreatic ?-cells.
FEBS letters588(14)2217-22(2014 Jun)
Milochau A,Lagr?e V,Benassy MN,Chaignepain S,Papin J,Garcia-Arcos I,Lajoix A,Monterrat C,Coudert L,Schmitter JM,Ochoa B,Lang J

Structural polymorphism in the N-terminal oligomerization domain of NPM1.
Proceedings of the National Academy of Sciences of the United States of America111(12)4466-71(2014 Mar)
Mitrea DM,Grace CR,Buljan M,Yun MK,Pytel NJ,Satumba J,Nourse A,Park CG,Madan Babu M,White SW,Kriwacki RW

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Npm1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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