Mip cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mip
Entrez Gene ID	25480
Full Name	major intrinsic protein of lens fiber
Synonyms	Aqp0
General protein information	Preferred Names major intrinsic protein of lens fiber Names lens fiber major intrinsic protein MIP26 MP26 aquaporin-0 major intrinsic protein of eye lens fiber
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	human homolog is a member of the aquaporin family of water transporters [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105719.1	NP_001099189.1	lens fiber major intrinsic protein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mip	NP_001099189.1
Pan troglodytes (chimpanzee)	MIP	XP_001168857.1
Danio rerio (zebrafish)	mipa	NP_001003534.1
Danio rerio (zebrafish)	mipb	NP_001018356.1
Arabidopsis thaliana (thale cress)	TIP2	NP_189283.1
Arabidopsis thaliana (thale cress)	GAMMA-TIP	NP_181221.1
	MIP	XP_001115118.1
Canis lupus familiaris (dog)	MIP	NP_001074369.1
Homo sapiens (human)	MIP	NP_036196.1
Bos taurus (cattle)	MIP	NP_776362.1
Mus musculus (house mouse)	Mip	NP_032626.2
Xenopus tropicalis (tropical clawed frog)	mip	NP_001090816.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional characterization of an AQP0 missense mutation, R33C, that causes dominant congenital lens cataract, reveals impaired cell-to-cell adhesion.
Kumari SS, Gandhi J, Mustehsan MH, Eren S, Varadaraj K
Experimental eye research116371-85(2013 Nov)

A 5-bp insertion in Mip causes recessive congenital cataract in KFRS4/Kyo rats.
Watanabe K, Wada K, Ohashi T, Okubo S, Takekuma K, Hashizume R, Hayashi J, Serikawa T, Kuramoto T, Kikkawa Y
PloS one7(11)e50737(2012)

Quantitative analysis of ascorbic acid permeability of aquaporin 0 in the lens.
Nakazawa Y, Oka M, Mitsuishi A, Bando M, Takehana M
Biochemical and biophysical research communications415(1)125-30(2011 Nov)

Water permeability and characterization of aquaporin-11.
Yakata K, Tani K, Fujiyoshi Y
Journal of structural biology174(2)315-20(2011 May)

Differentiation-dependent modification and subcellular distribution of aquaporin-0 suggests multiple functional roles in the rat lens.
Grey AC, Li L, Jacobs MD, Schey KL, Donaldson PJ
Differentiation; research in biological diversity77(1)70-83(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the kfrs4 rat represents the first characterised rat model with a recessive mutation in the Mip gene
Title: A 5-bp insertion in Mip causes recessive congenital cataract in KFRS4/Kyo rats.

These findings suggest that AQP0 plays an important role in controlling lens ascorbic acid content.
Title: Quantitative analysis of ascorbic acid permeability of aquaporin 0 in the lens.

These results show that changes in the subcellular distribution and processing of AQP0 occur at discrete stages during fiber cell differentiation in the eye lens and suggest that these stages of differentiation.
Title: Differentiation-dependent modification and subcellular distribution of aquaporin-0 suggests multiple functional roles in the rat lens.

These results indicate that serine(235) is required for proper intracellular transport of MIP/AQP0 from the trans-Golgi network to the plasma membrane.
Title: PKC putative phosphorylation site Ser235 is required for MIP/AQP0 translocation to the plasma membrane.

In addition to the lens, aquaporin-0 was expressed in the neural retina, the nuclei and the synaptic terminals of protein kinase alpha- and beta-expressing bipolar and amacrine cells, and by the nuclei of neuronal cells in the ganglion cell layer.
Title: Localization of aquaporin-0 immunoreactivity in the rat retina.

The following Mip gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mip cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11244	NM_001105719.1 Latest version!	Rattus norvegicus major intrinsic protein of lens fiber (Mip), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mip ( NM_001105719.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099189.1 Rattus norvegicus major intrinsic protein of lens fiber (Mip), mRNA.

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CloneID	ORa11244
Clone ID Related Accession (Same CDS sequence)	NM_001105719.1
Accession Version	NM_001105719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	lens fiber major intrinsic protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474104.2. On or before Oct 3, 2007 this sequence version replaced XM_343137.3, XM_001070399.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTGGGAAC TTCGGTCTGC CTCCTTTTGG AGGGCCATAT TTGCTGAGTT CTTTGCTACC 
CTCTTCTATG TCTTCTTTGG GCTGGGATCC TCACTGCGTT GGGCCCCTGG ACCCCTACAT 
GTCCTGCAAG TGGCTTTGGC CTTTGGCCTG GCCCTGGCCA CACTGGTGCA GACTGTGGGT 
CACATCAGTG GAGCCCATGT CAACCCTGCG GTCACTTTTG CCTTCCTTGT GGGCTCCCAG 
ATGTCCTTGC TCCGTGCTTT CTGCTATATA GCAGCCCAGC TTCTGGGAGC TGTGGCTGGA 
GCGGCTGTGC TGTACAGTGT CACCCCACCA GCTGTCCGAG GAAACCTAGC ACTCAACACG 
CTGCATGCTG GGGTAAGCGT GGGCCAGGCC ACCACTGTGG AGATCTTCCT GACGCTCCAG 
TTCGTGCTCT GCATCTTTGC TACGTATGAC GAGAGGCGGA ATGGCCGCAT GGGCTCTGTG 
GCCCTGGCTG TTGGCTTCTC CCTCACCCTG GGGCACCTCT TTGGGATGTA TTACACTGGC 
GCGGGGATGA ATCCCGCCCG CTCCTTTGCT CCTGCTATCC TCACCAGGAA CTTCAGCAAC 
CACTGGGTGT ACTGGGTGGG CCCAATCATC GGCGGGGGCC TGGGCAGCCT GCTCTACGAC 
TTTCTCCTCT TCCCACGGCT CAAGAGTGTT TCTGAGAGAC TGTCTATCCT CAAGGGAGCC 
AGACCCAGTG ACTCCAATGG ACAGCCAGAG GGTACAGGGG AACCTGTGGA ACTGAAGACT 
CAGGCCCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099189.1
CDS	46..837
Translation	MWELRSASFWRAIFAEFFATLFYVFFGLGSSLRWAPGPLHVLQVALAFGLALATLVQTVGHISGAHVNPAVTFAFLVGSQMSLLRAFCYIAAQLLGAVAGAAVLYSVTPPAVRGNLALNTLHAGVSVGQATTVEIFLTLQFVLCIFATYDERRNGRMGSVALAVGFSLTLGHLFGMYYTGAGMNPARSFAPAILTRNFSNHWVYWVGPIIGGGLGSLLYDFLLFPRLKSVSERLSILKGARPSDSNGQPEGTGEPVELKTQAL

Target ORF information:

RefSeq Version	NM_001105719.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus major intrinsic protein of lens fiber (Mip), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105719.1

ORF Insert Sequence:

ATGTGGGAAC TTCGGTCTGC CTCCTTTTGG AGGGCCATAT TTGCTGAGTT CTTTGCTACC 
CTCTTCTATG TCTTCTTTGG GCTGGGATCC TCACTGCGTT GGGCCCCTGG ACCCCTACAT 
GTCCTGCAAG TGGCTTTGGC CTTTGGCCTG GCCCTGGCCA CACTGGTGCA GACTGTGGGT 
CACATCAGTG GAGCCCATGT CAACCCTGCG GTCACTTTTG CCTTCCTTGT GGGCTCCCAG 
ATGTCCTTGC TCCGTGCTTT CTGCTATATA GCAGCCCAGC TTCTGGGAGC TGTGGCTGGA 
GCGGCTGTGC TGTACAGTGT CACCCCACCA GCTGTCCGAG GAAACCTAGC ACTCAACACG 
CTGCATGCTG GGGTAAGCGT GGGCCAGGCC ACCACTGTGG AGATCTTCCT GACGCTCCAG 
TTCGTGCTCT GCATCTTTGC TACGTATGAC GAGAGGCGGA ATGGCCGCAT GGGCTCTGTG 
GCCCTGGCTG TTGGCTTCTC CCTCACCCTG GGGCACCTCT TTGGGATGTA TTACACTGGC 
GCGGGGATGA ATCCCGCCCG CTCCTTTGCT CCTGCTATCC TCACCAGGAA CTTCAGCAAC 
CACTGGGTGT ACTGGGTGGG CCCAATCATC GGCGGGGGCC TGGGCAGCCT GCTCTACGAC 
TTTCTCCTCT TCCCACGGCT CAAGAGTGTT TCTGAGAGAC TGTCTATCCT CAAGGGAGCC 
AGACCCAGTG ACTCCAATGG ACAGCCAGAG GGTACAGGGG AACCTGTGGA ACTGAAGACT 
CAGGCCCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional characterization of an AQP0 missense mutation, R33C, that causes dominant congenital lens cataract, reveals impaired cell-to-cell adhesion.
Experimental eye research116371-85(2013 Nov)
Kumari SS,Gandhi J,Mustehsan MH,Eren S,Varadaraj K

A 5-bp insertion in Mip causes recessive congenital cataract in KFRS4/Kyo rats.
PloS one7(11)e50737(2012)
Watanabe K,Wada K,Ohashi T,Okubo S,Takekuma K,Hashizume R,Hayashi J,Serikawa T,Kuramoto T,Kikkawa Y

Quantitative analysis of ascorbic acid permeability of aquaporin 0 in the lens.
Biochemical and biophysical research communications415(1)125-30(2011 Nov)
Nakazawa Y,Oka M,Mitsuishi A,Bando M,Takehana M

Water permeability and characterization of aquaporin-11.
Journal of structural biology174(2)315-20(2011 May)
Yakata K,Tani K,Fujiyoshi Y

Differentiation-dependent modification and subcellular distribution of aquaporin-0 suggests multiple functional roles in the rat lens.
Differentiation; research in biological diversity77(1)70-83(2009 Jan)
Grey AC,Li L,Jacobs MD,Schey KL,Donaldson PJ

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Mip cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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