Kcna4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcna4
Entrez Gene ID	25469
Full Name	potassium voltage-gated channel subfamily A member 4
Synonyms	KCHAN,Kv1.4,Kv4,RHK1,RK3
General protein information	Preferred Names potassium voltage-gated channel subfamily A member 4 Names potassium voltage-gated channel subfamily A member 4 RCK4 potassium channel, voltage gated shaker related subfamily A, member 4 potassium voltage-gated channel, shaker-related subfamily, member 4 voltage-gated potassium channel subunit Kv1.4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q33
Summary	voltage-gated K+ channel; responsible for potassium permeability in excitable membranes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006234673.3	XP_006234735.1	potassium voltage-gated channel subfamily A member 4 isoform X1
XM_006234674.3	XP_006234736.1	potassium voltage-gated channel subfamily A member 4 isoform X1
XM_017591493.1	XP_017446982.1	potassium voltage-gated channel subfamily A member 4 isoform X1
NM_012971.2	NP_037103.1	potassium voltage-gated channel subfamily A member 4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KCNA4	NP_776796.1
Gallus gallus (chicken)	KCNA4	NP_990230.1
Homo sapiens (human)	KCNA4	NP_002224.1
Pan troglodytes (chimpanzee)	KCNA4	XP_003313022.1
	KCNA4	XP_001088267.1
Canis lupus familiaris (dog)	KCNA4	XP_005633823.1
Mus musculus (house mouse)	Kcna4	NP_067250.2
Xenopus tropicalis (tropical clawed frog)	kcna4	XP_004913510.1
Rattus norvegicus (Norway rat)	Kcna4	NP_037103.1
Danio rerio (zebrafish)	kcna4	XP_005169006.1

Gene Ontology
Bibliography

Related articles in PubMed

Amino Acid Properties of Trafficking Determinants in the Outer Pore-Forming Region of Kv1 Potassium Channels in Cell Lines.
Gomez B, Zhu J, Recio-Pinto E, Thornhill WB
Cell biochemistry and biophysics75(1)25-33(2017 Mar)

Synaptotagmin I delays the fast inactivation of Kv1.4 channel through interaction with its N-terminus.
Xie C, Su H, Guo T, Yan Y, Peng X, Cao R, Wang Y, Chen P, Wang X, Liang S
Molecular brain74(2014 Jan)

Heme impairs the ball-and-chain inactivation of potassium channels.
Sahoo N, Goradia N, Ohlenschl?ger O, Sch?nherr R, Friedrich M, Plass W, Kappl R, Hoshi T, Heinemann SH
Proceedings of the National Academy of Sciences of the United States of America110(42)E4036-44(2013 Oct)

Hydrogen sulfide increases excitability through suppression of sustained potassium channel currents of rat trigeminal ganglion neurons.
Feng X, Zhou YL, Meng X, Qi FH, Chen W, Jiang X, Xu GY
Molecular pain94(2013 Feb)

KCNE1 and KCNE2 provide a checkpoint governing voltage-gated potassium channel ?-subunit composition.
Kanda VA, Lewis A, Xu X, Abbott GW
Biophysical journal101(6)1364-75(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Four prolines and four lysines in a Kv1.4 homotetramer might provide a binding site for a putative endoplasmic reticulum-export molecule to ensure high cell surface protein expression of the channel.
Title: Amino Acid Properties of Trafficking Determinants in the Outer Pore-Forming Region of Kv1 Potassium Channels in Cell Lines.

synaptotagmin I is an interacting protein of Kv1.4 channel.
Title: Synaptotagmin I delays the fast inactivation of Kv1.4 channel through interaction with its N-terminus.

Kv1.4 K(+) channels are potently regulated by intracellular free heme; heme binds to the N-terminal inactivation domain and thereby impairs the inactivation process
Title: Heme impairs the ball-and-chain inactivation of potassium channels.

Endogenous H2S generating enzyme cystathionine-beta-synthetase was co-localized well with Kv1.1 and Kv1.4 in trigeminal ganglion neurons.
Title: Hydrogen sulfide increases excitability through suppression of sustained potassium channel currents of rat trigeminal ganglion neurons.

Immunocytochemistry indicated that protein expression for Kv1.4 increased between 1 wk and 4-5 wk of age.
Title: Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons.

The following Kcna4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcna4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13087	NM_012971.2 Latest version!	Rattus norvegicus potassium voltage-gated channel subfamily A member 4 (Kcna4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$384.30 $549.00
ORa26162	XM_006234673.3 Latest version!	Rattus norvegicus potassium voltage-gated channel subfamily A member 4 (Kcna4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa26162	XM_017591493.1 Latest version!	Rattus norvegicus potassium voltage-gated channel subfamily A member 4 (Kcna4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa26162	XM_006234674.3 Latest version!	Rattus norvegicus potassium voltage-gated channel subfamily A member 4 (Kcna4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcna4 ( NM_012971.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037103.1 Rattus norvegicus potassium voltage-gated channel subfamily A member 4 (Kcna4), mRNA.

Price & Availability↑

CloneID	ORa13087
Clone ID Related Accession (Same CDS sequence)	NM_012971.2
Accession Version	NM_012971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	potassium voltage-gated channel subfamily A member 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M32867.1. On Apr 12, 2007 this sequence version replaced NM_012971.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M32867.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAGGTGG CAATGGTGAG TGCCGAGAGC TCAGGGTGCA ACAGCCACAT GCCTTATGGT 
TATGCTGCCC AGGCCAGGGC TCGAGAGAGG GAGAGACTTG CTCACTCCAG GGCAGCTGCA 
GCTCTGGCTG TTGCAGCTGC CACGGCTGCG GTGGAAGGCA CTGGAGGTTC TGGTGGAGGC 
CCCCACCATC ATCATCAGAC ACGTGGGGCC TACTCCTCCC ATGATCCTCA AGGAAGCCGA 
GGTAGTCGGA GGAGGAGGCG ACAGCGAACT GAGAAGAAGA AACTCCACCA CAGGCAGAGC 
AGTTTTCCTC ATTGCTCAGA CCTGATGCCC AGTGGCTCTG AAGAGAAGAT CCTTAGGGAG 
CTGAGCGAGG AGGAGGAAGA CGAGGAGGAG GAAGAGGAGG AGGAAGAGGA GGGAAGGTTT 
TACTATAGTG AAGAGGACCA TGGGGATGGG TGTTCCTACA CTGACCTACT GCCACAGGAT 
GATGGGGGTG GCGGCGGCTA CAGTTCAGTC CGCTACAGTG ACTGTTGTGA ACGCGTGGTA 
ATAAATGTGT CTGGTCTACG CTTCGAAACC CAAATGAAAA CTTTGGCTCA GTTTCCAGAA 
ACTCTGTTGG GAGACCCTGA GAAGAGGACT CAGTACTTCG ACCCTTTGCG CAATGAGTAT 
TTTTTTGATA GGAACCGTCC CAGCTTTGAT GCCATTTTGT ATTATTACCA GTCAGGAGGC 
CGCCTGAAGA GGCCAGTCAA TGTCCCCTTT GATATCTTCA CTGAGGAGGT GAAGTTCTAT 
CAGTTGGGAG AGGAAGCCCT GCTCAAGTTC CGTGAGGATG AGGGCTTTGT GAGAGAAGAG 
GAGGACAGGG CTCTGCCAGA AAATGAATTT AAAAAACAGA TTTGGCTTCT CTTTGAATAT 
CCCGAGAGTT CCAGCCCTGC CAGGGCGATA GCCATCGTAT CTGTCCTGGT CATCTTAATC 
TCTATTGTCA TATTTTGCCT GGAAACCTTG CCTGAGTTCA GGGATGATAG GGACCTCATC 
ATGGCCCTCA GCGCAGGTGG ACACAGCAGA TTATTGAATG ACACCTCGGC ACCCCACCTG 
GAGAACTCAG GGCACACAAT ATTCAATGAC CCTTTCTTCA TTGTGGAGAC AGTATGTATC 
GTGTGGTTTT CCTTTGAGTT TGTGGTTCGA TGCTTTGCTT GTCCCAGTCA AGCACTCTTC 
TTCAAAAACA TCATGAACAT CATTGATATC GTCTCCATTT TGCCTTACTT CATCACTCTG 
GGCACCGATC TGGCCCAGCA GCAGGGGGGT GGCAACGGCC AGCAGCAGCA GGCTATGTCC 
TTTGCCATCC TCAGGATCAT CCGTCTGGTC CGAGTGTTCC GGATCTTCAA GCTCTCCAGA 
CACTCCAAGG GCCTGCAGAT CCTGGGCCAC ACCCTAAGAG CCAGCATGCG TGAACTGGGC 
CTTCTTATCT TTTTCCTCTT CATCGGGGTC ATCCTCTTTT CCAGCGCTGT GTATTTTGCA 
GAGGCAGATG AACCTACCAC CCATTTCCAA AGCATTCCAG ATGCGTTTTG GTGGGCTGTG 
GTAACCATGA CAACTGTGGG CTACGGGGAC ATGAAGCCCA TCACAGTGGG AGGAAAGATT 
GTGGGGTCCC TGTGTGCCAT TGCGGGTGTC TTAACCATTG CTTTGCCCGT GCCGGTGATT 
GTGTCTAACT TTAACTATTT CTACCACAGA GAGACTGAAA ACGAAGAACA GACCCAGCTG 
ACCCAAAACG CAGTCAGTTG CCCATACCTA CCTTCTAATT TGCTCAAGAA ATTTCGGAGC 
TCTACTTCTT CTTCCCTGGG GGACAAGTCA GAGTATCTAG AGATGGAAGA AGGGGTCAAG 
GAGTCTTTAT GTGGAAAGGA AGAGAAGTGT CAGGGAAAGG GGGATGACAG CGAGACAGAT 
AAAAACAACT GTTCTAATGC AAAGGCTGTG GAGACTGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037103.1
CDS	81..2045
Translation	MEVAMVSAESSGCNSHMPYGYAAQARARERERLAHSRAAAALAVAAATAAVEGTGGSGGGPHHHHQTRGAYSSHDPQGSRGSRRRRRQRTEKKKLHHRQSSFPHCSDLMPSGSEEKILRELSEEEEDEEEEEEEEEEGRFYYSEEDHGDGCSYTDLLPQDDGGGGGYSSVRYSDCCERVVINVSGLRFETQMKTLAQFPETLLGDPEKRTQYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLKRPVNVPFDIFTEEVKFYQLGEEALLKFREDEGFVREEEDRALPENEFKKQIWLLFEYPESSSPARAIAIVSVLVILISIVIFCLETLPEFRDDRDLIMALSAGGHSRLLNDTSAPHLENSGHTIFNDPFFIVETVCIVWFSFEFVVRCFACPSQALFFKNIMNIIDIVSILPYFITLGTDLAQQQGGGNGQQQQAMSFAILRIIRLVRVFRIFKLSRHSKGLQILGHTLRASMRELGLLIFFLFIGVILFSSAVYFAEADEPTTHFQSIPDAFWWAVVTMTTVGYGDMKPITVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETENEEQTQLTQNAVSCPYLPSNLLKKFRSSTSSSLGDKSEYLEMEEGVKESLCGKEEKCQGKGDDSETDKNNCSNAKAVETDV

Target ORF information:

RefSeq Version	NM_012971.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium voltage-gated channel subfamily A member 4 (Kcna4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012971.2

ORF Insert Sequence:

ATGGAGGTGG CAATGGTGAG TGCCGAGAGC TCAGGGTGCA ACAGCCACAT GCCTTATGGT 
TATGCTGCCC AGGCCAGGGC TCGAGAGAGG GAGAGACTTG CTCACTCCAG GGCAGCTGCA 
GCTCTGGCTG TTGCAGCTGC CACGGCTGCG GTGGAAGGCA CTGGAGGTTC TGGTGGAGGC 
CCCCACCATC ATCATCAGAC ACGTGGGGCC TACTCCTCCC ATGATCCTCA AGGAAGCCGA 
GGTAGTCGGA GGAGGAGGCG ACAGCGAACT GAGAAGAAGA AACTCCACCA CAGGCAGAGC 
AGTTTTCCTC ATTGCTCAGA CCTGATGCCC AGTGGCTCTG AAGAGAAGAT CCTTAGGGAG 
CTGAGCGAGG AGGAGGAAGA CGAGGAGGAG GAAGAGGAGG AGGAAGAGGA GGGAAGGTTT 
TACTATAGTG AAGAGGACCA TGGGGATGGG TGTTCCTACA CTGACCTACT GCCACAGGAT 
GATGGGGGTG GCGGCGGCTA CAGTTCAGTC CGCTACAGTG ACTGTTGTGA ACGCGTGGTA 
ATAAATGTGT CTGGTCTACG CTTCGAAACC CAAATGAAAA CTTTGGCTCA GTTTCCAGAA 
ACTCTGTTGG GAGACCCTGA GAAGAGGACT CAGTACTTCG ACCCTTTGCG CAATGAGTAT 
TTTTTTGATA GGAACCGTCC CAGCTTTGAT GCCATTTTGT ATTATTACCA GTCAGGAGGC 
CGCCTGAAGA GGCCAGTCAA TGTCCCCTTT GATATCTTCA CTGAGGAGGT GAAGTTCTAT 
CAGTTGGGAG AGGAAGCCCT GCTCAAGTTC CGTGAGGATG AGGGCTTTGT GAGAGAAGAG 
GAGGACAGGG CTCTGCCAGA AAATGAATTT AAAAAACAGA TTTGGCTTCT CTTTGAATAT 
CCCGAGAGTT CCAGCCCTGC CAGGGCGATA GCCATCGTAT CTGTCCTGGT CATCTTAATC 
TCTATTGTCA TATTTTGCCT GGAAACCTTG CCTGAGTTCA GGGATGATAG GGACCTCATC 
ATGGCCCTCA GCGCAGGTGG ACACAGCAGA TTATTGAATG ACACCTCGGC ACCCCACCTG 
GAGAACTCAG GGCACACAAT ATTCAATGAC CCTTTCTTCA TTGTGGAGAC AGTATGTATC 
GTGTGGTTTT CCTTTGAGTT TGTGGTTCGA TGCTTTGCTT GTCCCAGTCA AGCACTCTTC 
TTCAAAAACA TCATGAACAT CATTGATATC GTCTCCATTT TGCCTTACTT CATCACTCTG 
GGCACCGATC TGGCCCAGCA GCAGGGGGGT GGCAACGGCC AGCAGCAGCA GGCTATGTCC 
TTTGCCATCC TCAGGATCAT CCGTCTGGTC CGAGTGTTCC GGATCTTCAA GCTCTCCAGA 
CACTCCAAGG GCCTGCAGAT CCTGGGCCAC ACCCTAAGAG CCAGCATGCG TGAACTGGGC 
CTTCTTATCT TTTTCCTCTT CATCGGGGTC ATCCTCTTTT CCAGCGCTGT GTATTTTGCA 
GAGGCAGATG AACCTACCAC CCATTTCCAA AGCATTCCAG ATGCGTTTTG GTGGGCTGTG 
GTAACCATGA CAACTGTGGG CTACGGGGAC ATGAAGCCCA TCACAGTGGG AGGAAAGATT 
GTGGGGTCCC TGTGTGCCAT TGCGGGTGTC TTAACCATTG CTTTGCCCGT GCCGGTGATT 
GTGTCTAACT TTAACTATTT CTACCACAGA GAGACTGAAA ACGAAGAACA GACCCAGCTG 
ACCCAAAACG CAGTCAGTTG CCCATACCTA CCTTCTAATT TGCTCAAGAA ATTTCGGAGC 
TCTACTTCTT CTTCCCTGGG GGACAAGTCA GAGTATCTAG AGATGGAAGA AGGGGTCAAG 
GAGTCTTTAT GTGGAAAGGA AGAGAAGTGT CAGGGAAAGG GGGATGACAG CGAGACAGAT 
AAAAACAACT GTTCTAATGC AAAGGCTGTG GAGACTGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcna4 ( XM_006234673.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234735.1 Rattus norvegicus potassium voltage-gated channel subfamily A member 4 (Kcna4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa26162
Clone ID Related Accession (Same CDS sequence)	XM_006234673.3 , XM_006234674.3 , XM_017591493.1
Accession Version	XM_006234673.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium voltage-gated channel subfamily A member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG CAATGGTGAG TGCCGAGAGC TCAGGGTGCA ACAGCCACAT GCCTTATGGT 
TATGCTGCCC AGGCCAGGGC TCGAGAGAGG GAGAGACTTG CTCACTCCAG GGCAGCTGCA 
GCTGCTGCTG TTGCAGCTGC CACGGCTGCG GTGGAAGGCA CTGGAGGTTC TGGTGGAGGC 
CCCCACCATC ATCATCAGAC ACGTGGGGCC TACTCCTCCC ATGATCCTCA AGGAAGCCGA 
GGTAGTCGGA GGAGGAGGCG ACAGCGAACT GAGAAGAAGA AACTCCACCA CAGGCAGAGC 
AGTTTTCCTC ATTGCTCAGA CCTGATGCCC AGTGGCTCTG AAGAGAAGAT CCTTAGGGAG 
CTGAGCGAGG AGGAGGAAGA CGAGGAGGAG GAAGAGGAGG AGGAAGAGGA GGGAAGGTTT 
TACTATAGTG AAGAGGACCA TGGGGATGGG TGTTCCTACA CTGACCTACT GCCACAGGAT 
GATGGGGGTG GCGGCGGCTA CAGTTCAGTC CGCTACAGTG ACTGTTGTGA ACGCGTGGTA 
ATAAATGTGT CTGGTCTACG CTTCGAAACC CAAATGAAAA CTTTGGCTCA GTTTCCAGAA 
ACTCTGTTGG GAGACCCTGA GAAGAGGACT CAGTACTTCG ACCCTTTGCG CAATGAGTAT 
TTTTTTGATA GGAACCGTCC CAGCTTTGAT GCCATTTTGT ATTATTACCA GTCAGGAGGC 
CGCCTGAAGA GGCCAGTCAA TGTCCCCTTT GATATCTTCA CTGAGGAGGT GAAGTTCTAT 
CAGTTGGGAG AGGAAGCCCT GCTCAAGTTC CGTGAGGATG AGGGCTTTGT GAGAGAAGAG 
GAGGACAGGG CTCTGCCAGA AAATGAATTT AAAAAACAGA TTTGGCTTCT CTTTGAATAT 
CCCGAGAGTT CCAGCCCTGC CAGGGGCATA GCCATCGTAT CTGTCCTGGT CATCTTAATC 
TCTATTGTCA TATTTTGCCT GGAAACCTTG CCTGAGTTCA GGGATGATAG GGACCTCATC 
ATGGCCCTCA GCGCAGGTGG ACACAGCAGA TTATTGAATG ACACCTCGGC ACCCCACCTG 
GAGAACTCAG GGCACACAAT ATTCAATGAC CCTTTCTTCA TTGTGGAGAC AGTATGTATC 
GTGTGGTTTT CCTTTGAGTT TGTGGTTCGA TGCTTTGCTT GTCCCAGTCA AGCACTCTTC 
TTCAAAAACA TCATGAACAT CATTGATATC GTCTCCATTT TGCCTTACTT CATCACTCTG 
GGCACCGATC TGGCCCAGCA GCAGGGGGGT GGCAACGGCC AGCAGCAGCA GGCTATGTCC 
TTTGCCATCC TCAGGATCAT CCGTCTGGTC CGAGTGTTCC GGATCTTCAA GCTCTCCAGA 
CACTCCAAGG GCCTGCAGAT CCTGGGCCAC ACCCTAAGAG CCAGCATGCG TGAACTGGGC 
CTTCTTATCT TTTTCCTCTT CATCGGGGTC ATCCTCTTTT CCAGCGCTGT GTATTTTGCA 
GAGGCAGATG AACCTACCAC CCATTTCCAA AGCATTCCAG ATGCGTTTTG GTGGGCTGTG 
GTAACCATGA CAACTGTGGG CTACGGGGAC ATGAAGCCCA TCACAGTGGG AGGAAAGATT 
GTGGGGTCCC TGTGTGCCAT TGCGGGTGTC TTAACCATTG CTTTGCCCGT GCCGGTGATT 
GTGTCTAACT TTAACTATTT CTACCACAGA GAGACTGAAA ACGAAGAACA GACCCAGCTG 
ACCCAAAACG CAGTCAGTTG CCCATACCTA CCTTCTAATT TGCTCAAGAA ATTTCGGAGC 
TCTACTTCTT CTTCCCTGGG GGACAAGTCA GAGTATCTAG AGATGGAAGA AGGGGTCAAG 
GAGTCTTTAT GTGGAAAGGA AGAGAAGTGT CAGGGAAAGG GGGATGACAG CGAGACAGAT 
AAAAACAACT GTTCTAATGC AAAGGCTGTG GAGACTGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234735.1
CDS	1280..3244
Translation	MEVAMVSAESSGCNSHMPYGYAAQARARERERLAHSRAAAAAAVAAATAAVEGTGGSGGGPHHHHQTRGAYSSHDPQGSRGSRRRRRQRTEKKKLHHRQSSFPHCSDLMPSGSEEKILRELSEEEEDEEEEEEEEEEGRFYYSEEDHGDGCSYTDLLPQDDGGGGGYSSVRYSDCCERVVINVSGLRFETQMKTLAQFPETLLGDPEKRTQYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLKRPVNVPFDIFTEEVKFYQLGEEALLKFREDEGFVREEEDRALPENEFKKQIWLLFEYPESSSPARGIAIVSVLVILISIVIFCLETLPEFRDDRDLIMALSAGGHSRLLNDTSAPHLENSGHTIFNDPFFIVETVCIVWFSFEFVVRCFACPSQALFFKNIMNIIDIVSILPYFITLGTDLAQQQGGGNGQQQQAMSFAILRIIRLVRVFRIFKLSRHSKGLQILGHTLRASMRELGLLIFFLFIGVILFSSAVYFAEADEPTTHFQSIPDAFWWAVVTMTTVGYGDMKPITVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETENEEQTQLTQNAVSCPYLPSNLLKKFRSSTSSSLGDKSEYLEMEEGVKESLCGKEEKCQGKGDDSETDKNNCSNAKAVETDV

Target ORF information:

RefSeq Version	XM_006234673.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium voltage-gated channel subfamily A member 4 (Kcna4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234673.3

ORF Insert Sequence:

ATGGAGGTGG CAATGGTGAG TGCCGAGAGC TCAGGGTGCA ACAGCCACAT GCCTTATGGT 
TATGCTGCCC AGGCCAGGGC TCGAGAGAGG GAGAGACTTG CTCACTCCAG GGCAGCTGCA 
GCTGCTGCTG TTGCAGCTGC CACGGCTGCG GTGGAAGGCA CTGGAGGTTC TGGTGGAGGC 
CCCCACCATC ATCATCAGAC ACGTGGGGCC TACTCCTCCC ATGATCCTCA AGGAAGCCGA 
GGTAGTCGGA GGAGGAGGCG ACAGCGAACT GAGAAGAAGA AACTCCACCA CAGGCAGAGC 
AGTTTTCCTC ATTGCTCAGA CCTGATGCCC AGTGGCTCTG AAGAGAAGAT CCTTAGGGAG 
CTGAGCGAGG AGGAGGAAGA CGAGGAGGAG GAAGAGGAGG AGGAAGAGGA GGGAAGGTTT 
TACTATAGTG AAGAGGACCA TGGGGATGGG TGTTCCTACA CTGACCTACT GCCACAGGAT 
GATGGGGGTG GCGGCGGCTA CAGTTCAGTC CGCTACAGTG ACTGTTGTGA ACGCGTGGTA 
ATAAATGTGT CTGGTCTACG CTTCGAAACC CAAATGAAAA CTTTGGCTCA GTTTCCAGAA 
ACTCTGTTGG GAGACCCTGA GAAGAGGACT CAGTACTTCG ACCCTTTGCG CAATGAGTAT 
TTTTTTGATA GGAACCGTCC CAGCTTTGAT GCCATTTTGT ATTATTACCA GTCAGGAGGC 
CGCCTGAAGA GGCCAGTCAA TGTCCCCTTT GATATCTTCA CTGAGGAGGT GAAGTTCTAT 
CAGTTGGGAG AGGAAGCCCT GCTCAAGTTC CGTGAGGATG AGGGCTTTGT GAGAGAAGAG 
GAGGACAGGG CTCTGCCAGA AAATGAATTT AAAAAACAGA TTTGGCTTCT CTTTGAATAT 
CCCGAGAGTT CCAGCCCTGC CAGGGGCATA GCCATCGTAT CTGTCCTGGT CATCTTAATC 
TCTATTGTCA TATTTTGCCT GGAAACCTTG CCTGAGTTCA GGGATGATAG GGACCTCATC 
ATGGCCCTCA GCGCAGGTGG ACACAGCAGA TTATTGAATG ACACCTCGGC ACCCCACCTG 
GAGAACTCAG GGCACACAAT ATTCAATGAC CCTTTCTTCA TTGTGGAGAC AGTATGTATC 
GTGTGGTTTT CCTTTGAGTT TGTGGTTCGA TGCTTTGCTT GTCCCAGTCA AGCACTCTTC 
TTCAAAAACA TCATGAACAT CATTGATATC GTCTCCATTT TGCCTTACTT CATCACTCTG 
GGCACCGATC TGGCCCAGCA GCAGGGGGGT GGCAACGGCC AGCAGCAGCA GGCTATGTCC 
TTTGCCATCC TCAGGATCAT CCGTCTGGTC CGAGTGTTCC GGATCTTCAA GCTCTCCAGA 
CACTCCAAGG GCCTGCAGAT CCTGGGCCAC ACCCTAAGAG CCAGCATGCG TGAACTGGGC 
CTTCTTATCT TTTTCCTCTT CATCGGGGTC ATCCTCTTTT CCAGCGCTGT GTATTTTGCA 
GAGGCAGATG AACCTACCAC CCATTTCCAA AGCATTCCAG ATGCGTTTTG GTGGGCTGTG 
GTAACCATGA CAACTGTGGG CTACGGGGAC ATGAAGCCCA TCACAGTGGG AGGAAAGATT 
GTGGGGTCCC TGTGTGCCAT TGCGGGTGTC TTAACCATTG CTTTGCCCGT GCCGGTGATT 
GTGTCTAACT TTAACTATTT CTACCACAGA GAGACTGAAA ACGAAGAACA GACCCAGCTG 
ACCCAAAACG CAGTCAGTTG CCCATACCTA CCTTCTAATT TGCTCAAGAA ATTTCGGAGC 
TCTACTTCTT CTTCCCTGGG GGACAAGTCA GAGTATCTAG AGATGGAAGA AGGGGTCAAG 
GAGTCTTTAT GTGGAAAGGA AGAGAAGTGT CAGGGAAAGG GGGATGACAG CGAGACAGAT 
AAAAACAACT GTTCTAATGC AAAGGCTGTG GAGACTGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcna4 ( XM_006234674.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234736.1 Rattus norvegicus potassium voltage-gated channel subfamily A member 4 (Kcna4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa26162
Clone ID Related Accession (Same CDS sequence)	XM_006234673.3 , XM_006234674.3 , XM_017591493.1
Accession Version	XM_006234674.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium voltage-gated channel subfamily A member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234674.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG CAATGGTGAG TGCCGAGAGC TCAGGGTGCA ACAGCCACAT GCCTTATGGT 
TATGCTGCCC AGGCCAGGGC TCGAGAGAGG GAGAGACTTG CTCACTCCAG GGCAGCTGCA 
GCTGCTGCTG TTGCAGCTGC CACGGCTGCG GTGGAAGGCA CTGGAGGTTC TGGTGGAGGC 
CCCCACCATC ATCATCAGAC ACGTGGGGCC TACTCCTCCC ATGATCCTCA AGGAAGCCGA 
GGTAGTCGGA GGAGGAGGCG ACAGCGAACT GAGAAGAAGA AACTCCACCA CAGGCAGAGC 
AGTTTTCCTC ATTGCTCAGA CCTGATGCCC AGTGGCTCTG AAGAGAAGAT CCTTAGGGAG 
CTGAGCGAGG AGGAGGAAGA CGAGGAGGAG GAAGAGGAGG AGGAAGAGGA GGGAAGGTTT 
TACTATAGTG AAGAGGACCA TGGGGATGGG TGTTCCTACA CTGACCTACT GCCACAGGAT 
GATGGGGGTG GCGGCGGCTA CAGTTCAGTC CGCTACAGTG ACTGTTGTGA ACGCGTGGTA 
ATAAATGTGT CTGGTCTACG CTTCGAAACC CAAATGAAAA CTTTGGCTCA GTTTCCAGAA 
ACTCTGTTGG GAGACCCTGA GAAGAGGACT CAGTACTTCG ACCCTTTGCG CAATGAGTAT 
TTTTTTGATA GGAACCGTCC CAGCTTTGAT GCCATTTTGT ATTATTACCA GTCAGGAGGC 
CGCCTGAAGA GGCCAGTCAA TGTCCCCTTT GATATCTTCA CTGAGGAGGT GAAGTTCTAT 
CAGTTGGGAG AGGAAGCCCT GCTCAAGTTC CGTGAGGATG AGGGCTTTGT GAGAGAAGAG 
GAGGACAGGG CTCTGCCAGA AAATGAATTT AAAAAACAGA TTTGGCTTCT CTTTGAATAT 
CCCGAGAGTT CCAGCCCTGC CAGGGGCATA GCCATCGTAT CTGTCCTGGT CATCTTAATC 
TCTATTGTCA TATTTTGCCT GGAAACCTTG CCTGAGTTCA GGGATGATAG GGACCTCATC 
ATGGCCCTCA GCGCAGGTGG ACACAGCAGA TTATTGAATG ACACCTCGGC ACCCCACCTG 
GAGAACTCAG GGCACACAAT ATTCAATGAC CCTTTCTTCA TTGTGGAGAC AGTATGTATC 
GTGTGGTTTT CCTTTGAGTT TGTGGTTCGA TGCTTTGCTT GTCCCAGTCA AGCACTCTTC 
TTCAAAAACA TCATGAACAT CATTGATATC GTCTCCATTT TGCCTTACTT CATCACTCTG 
GGCACCGATC TGGCCCAGCA GCAGGGGGGT GGCAACGGCC AGCAGCAGCA GGCTATGTCC 
TTTGCCATCC TCAGGATCAT CCGTCTGGTC CGAGTGTTCC GGATCTTCAA GCTCTCCAGA 
CACTCCAAGG GCCTGCAGAT CCTGGGCCAC ACCCTAAGAG CCAGCATGCG TGAACTGGGC 
CTTCTTATCT TTTTCCTCTT CATCGGGGTC ATCCTCTTTT CCAGCGCTGT GTATTTTGCA 
GAGGCAGATG AACCTACCAC CCATTTCCAA AGCATTCCAG ATGCGTTTTG GTGGGCTGTG 
GTAACCATGA CAACTGTGGG CTACGGGGAC ATGAAGCCCA TCACAGTGGG AGGAAAGATT 
GTGGGGTCCC TGTGTGCCAT TGCGGGTGTC TTAACCATTG CTTTGCCCGT GCCGGTGATT 
GTGTCTAACT TTAACTATTT CTACCACAGA GAGACTGAAA ACGAAGAACA GACCCAGCTG 
ACCCAAAACG CAGTCAGTTG CCCATACCTA CCTTCTAATT TGCTCAAGAA ATTTCGGAGC 
TCTACTTCTT CTTCCCTGGG GGACAAGTCA GAGTATCTAG AGATGGAAGA AGGGGTCAAG 
GAGTCTTTAT GTGGAAAGGA AGAGAAGTGT CAGGGAAAGG GGGATGACAG CGAGACAGAT 
AAAAACAACT GTTCTAATGC AAAGGCTGTG GAGACTGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234736.1
CDS	1127..3091
Translation	MEVAMVSAESSGCNSHMPYGYAAQARARERERLAHSRAAAAAAVAAATAAVEGTGGSGGGPHHHHQTRGAYSSHDPQGSRGSRRRRRQRTEKKKLHHRQSSFPHCSDLMPSGSEEKILRELSEEEEDEEEEEEEEEEGRFYYSEEDHGDGCSYTDLLPQDDGGGGGYSSVRYSDCCERVVINVSGLRFETQMKTLAQFPETLLGDPEKRTQYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLKRPVNVPFDIFTEEVKFYQLGEEALLKFREDEGFVREEEDRALPENEFKKQIWLLFEYPESSSPARGIAIVSVLVILISIVIFCLETLPEFRDDRDLIMALSAGGHSRLLNDTSAPHLENSGHTIFNDPFFIVETVCIVWFSFEFVVRCFACPSQALFFKNIMNIIDIVSILPYFITLGTDLAQQQGGGNGQQQQAMSFAILRIIRLVRVFRIFKLSRHSKGLQILGHTLRASMRELGLLIFFLFIGVILFSSAVYFAEADEPTTHFQSIPDAFWWAVVTMTTVGYGDMKPITVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETENEEQTQLTQNAVSCPYLPSNLLKKFRSSTSSSLGDKSEYLEMEEGVKESLCGKEEKCQGKGDDSETDKNNCSNAKAVETDV

Target ORF information:

RefSeq Version	XM_006234674.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium voltage-gated channel subfamily A member 4 (Kcna4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234674.3

ORF Insert Sequence:

ATGGAGGTGG CAATGGTGAG TGCCGAGAGC TCAGGGTGCA ACAGCCACAT GCCTTATGGT 
TATGCTGCCC AGGCCAGGGC TCGAGAGAGG GAGAGACTTG CTCACTCCAG GGCAGCTGCA 
GCTGCTGCTG TTGCAGCTGC CACGGCTGCG GTGGAAGGCA CTGGAGGTTC TGGTGGAGGC 
CCCCACCATC ATCATCAGAC ACGTGGGGCC TACTCCTCCC ATGATCCTCA AGGAAGCCGA 
GGTAGTCGGA GGAGGAGGCG ACAGCGAACT GAGAAGAAGA AACTCCACCA CAGGCAGAGC 
AGTTTTCCTC ATTGCTCAGA CCTGATGCCC AGTGGCTCTG AAGAGAAGAT CCTTAGGGAG 
CTGAGCGAGG AGGAGGAAGA CGAGGAGGAG GAAGAGGAGG AGGAAGAGGA GGGAAGGTTT 
TACTATAGTG AAGAGGACCA TGGGGATGGG TGTTCCTACA CTGACCTACT GCCACAGGAT 
GATGGGGGTG GCGGCGGCTA CAGTTCAGTC CGCTACAGTG ACTGTTGTGA ACGCGTGGTA 
ATAAATGTGT CTGGTCTACG CTTCGAAACC CAAATGAAAA CTTTGGCTCA GTTTCCAGAA 
ACTCTGTTGG GAGACCCTGA GAAGAGGACT CAGTACTTCG ACCCTTTGCG CAATGAGTAT 
TTTTTTGATA GGAACCGTCC CAGCTTTGAT GCCATTTTGT ATTATTACCA GTCAGGAGGC 
CGCCTGAAGA GGCCAGTCAA TGTCCCCTTT GATATCTTCA CTGAGGAGGT GAAGTTCTAT 
CAGTTGGGAG AGGAAGCCCT GCTCAAGTTC CGTGAGGATG AGGGCTTTGT GAGAGAAGAG 
GAGGACAGGG CTCTGCCAGA AAATGAATTT AAAAAACAGA TTTGGCTTCT CTTTGAATAT 
CCCGAGAGTT CCAGCCCTGC CAGGGGCATA GCCATCGTAT CTGTCCTGGT CATCTTAATC 
TCTATTGTCA TATTTTGCCT GGAAACCTTG CCTGAGTTCA GGGATGATAG GGACCTCATC 
ATGGCCCTCA GCGCAGGTGG ACACAGCAGA TTATTGAATG ACACCTCGGC ACCCCACCTG 
GAGAACTCAG GGCACACAAT ATTCAATGAC CCTTTCTTCA TTGTGGAGAC AGTATGTATC 
GTGTGGTTTT CCTTTGAGTT TGTGGTTCGA TGCTTTGCTT GTCCCAGTCA AGCACTCTTC 
TTCAAAAACA TCATGAACAT CATTGATATC GTCTCCATTT TGCCTTACTT CATCACTCTG 
GGCACCGATC TGGCCCAGCA GCAGGGGGGT GGCAACGGCC AGCAGCAGCA GGCTATGTCC 
TTTGCCATCC TCAGGATCAT CCGTCTGGTC CGAGTGTTCC GGATCTTCAA GCTCTCCAGA 
CACTCCAAGG GCCTGCAGAT CCTGGGCCAC ACCCTAAGAG CCAGCATGCG TGAACTGGGC 
CTTCTTATCT TTTTCCTCTT CATCGGGGTC ATCCTCTTTT CCAGCGCTGT GTATTTTGCA 
GAGGCAGATG AACCTACCAC CCATTTCCAA AGCATTCCAG ATGCGTTTTG GTGGGCTGTG 
GTAACCATGA CAACTGTGGG CTACGGGGAC ATGAAGCCCA TCACAGTGGG AGGAAAGATT 
GTGGGGTCCC TGTGTGCCAT TGCGGGTGTC TTAACCATTG CTTTGCCCGT GCCGGTGATT 
GTGTCTAACT TTAACTATTT CTACCACAGA GAGACTGAAA ACGAAGAACA GACCCAGCTG 
ACCCAAAACG CAGTCAGTTG CCCATACCTA CCTTCTAATT TGCTCAAGAA ATTTCGGAGC 
TCTACTTCTT CTTCCCTGGG GGACAAGTCA GAGTATCTAG AGATGGAAGA AGGGGTCAAG 
GAGTCTTTAT GTGGAAAGGA AGAGAAGTGT CAGGGAAAGG GGGATGACAG CGAGACAGAT 
AAAAACAACT GTTCTAATGC AAAGGCTGTG GAGACTGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcna4 ( XM_017591493.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446982.1 Rattus norvegicus potassium voltage-gated channel subfamily A member 4 (Kcna4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa26162
Clone ID Related Accession (Same CDS sequence)	XM_006234673.3 , XM_006234674.3 , XM_017591493.1
Accession Version	XM_017591493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium voltage-gated channel subfamily A member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG CAATGGTGAG TGCCGAGAGC TCAGGGTGCA ACAGCCACAT GCCTTATGGT 
TATGCTGCCC AGGCCAGGGC TCGAGAGAGG GAGAGACTTG CTCACTCCAG GGCAGCTGCA 
GCTGCTGCTG TTGCAGCTGC CACGGCTGCG GTGGAAGGCA CTGGAGGTTC TGGTGGAGGC 
CCCCACCATC ATCATCAGAC ACGTGGGGCC TACTCCTCCC ATGATCCTCA AGGAAGCCGA 
GGTAGTCGGA GGAGGAGGCG ACAGCGAACT GAGAAGAAGA AACTCCACCA CAGGCAGAGC 
AGTTTTCCTC ATTGCTCAGA CCTGATGCCC AGTGGCTCTG AAGAGAAGAT CCTTAGGGAG 
CTGAGCGAGG AGGAGGAAGA CGAGGAGGAG GAAGAGGAGG AGGAAGAGGA GGGAAGGTTT 
TACTATAGTG AAGAGGACCA TGGGGATGGG TGTTCCTACA CTGACCTACT GCCACAGGAT 
GATGGGGGTG GCGGCGGCTA CAGTTCAGTC CGCTACAGTG ACTGTTGTGA ACGCGTGGTA 
ATAAATGTGT CTGGTCTACG CTTCGAAACC CAAATGAAAA CTTTGGCTCA GTTTCCAGAA 
ACTCTGTTGG GAGACCCTGA GAAGAGGACT CAGTACTTCG ACCCTTTGCG CAATGAGTAT 
TTTTTTGATA GGAACCGTCC CAGCTTTGAT GCCATTTTGT ATTATTACCA GTCAGGAGGC 
CGCCTGAAGA GGCCAGTCAA TGTCCCCTTT GATATCTTCA CTGAGGAGGT GAAGTTCTAT 
CAGTTGGGAG AGGAAGCCCT GCTCAAGTTC CGTGAGGATG AGGGCTTTGT GAGAGAAGAG 
GAGGACAGGG CTCTGCCAGA AAATGAATTT AAAAAACAGA TTTGGCTTCT CTTTGAATAT 
CCCGAGAGTT CCAGCCCTGC CAGGGGCATA GCCATCGTAT CTGTCCTGGT CATCTTAATC 
TCTATTGTCA TATTTTGCCT GGAAACCTTG CCTGAGTTCA GGGATGATAG GGACCTCATC 
ATGGCCCTCA GCGCAGGTGG ACACAGCAGA TTATTGAATG ACACCTCGGC ACCCCACCTG 
GAGAACTCAG GGCACACAAT ATTCAATGAC CCTTTCTTCA TTGTGGAGAC AGTATGTATC 
GTGTGGTTTT CCTTTGAGTT TGTGGTTCGA TGCTTTGCTT GTCCCAGTCA AGCACTCTTC 
TTCAAAAACA TCATGAACAT CATTGATATC GTCTCCATTT TGCCTTACTT CATCACTCTG 
GGCACCGATC TGGCCCAGCA GCAGGGGGGT GGCAACGGCC AGCAGCAGCA GGCTATGTCC 
TTTGCCATCC TCAGGATCAT CCGTCTGGTC CGAGTGTTCC GGATCTTCAA GCTCTCCAGA 
CACTCCAAGG GCCTGCAGAT CCTGGGCCAC ACCCTAAGAG CCAGCATGCG TGAACTGGGC 
CTTCTTATCT TTTTCCTCTT CATCGGGGTC ATCCTCTTTT CCAGCGCTGT GTATTTTGCA 
GAGGCAGATG AACCTACCAC CCATTTCCAA AGCATTCCAG ATGCGTTTTG GTGGGCTGTG 
GTAACCATGA CAACTGTGGG CTACGGGGAC ATGAAGCCCA TCACAGTGGG AGGAAAGATT 
GTGGGGTCCC TGTGTGCCAT TGCGGGTGTC TTAACCATTG CTTTGCCCGT GCCGGTGATT 
GTGTCTAACT TTAACTATTT CTACCACAGA GAGACTGAAA ACGAAGAACA GACCCAGCTG 
ACCCAAAACG CAGTCAGTTG CCCATACCTA CCTTCTAATT TGCTCAAGAA ATTTCGGAGC 
TCTACTTCTT CTTCCCTGGG GGACAAGTCA GAGTATCTAG AGATGGAAGA AGGGGTCAAG 
GAGTCTTTAT GTGGAAAGGA AGAGAAGTGT CAGGGAAAGG GGGATGACAG CGAGACAGAT 
AAAAACAACT GTTCTAATGC AAAGGCTGTG GAGACTGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446982.1
CDS	1933..3897
Translation	MEVAMVSAESSGCNSHMPYGYAAQARARERERLAHSRAAAAAAVAAATAAVEGTGGSGGGPHHHHQTRGAYSSHDPQGSRGSRRRRRQRTEKKKLHHRQSSFPHCSDLMPSGSEEKILRELSEEEEDEEEEEEEEEEGRFYYSEEDHGDGCSYTDLLPQDDGGGGGYSSVRYSDCCERVVINVSGLRFETQMKTLAQFPETLLGDPEKRTQYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLKRPVNVPFDIFTEEVKFYQLGEEALLKFREDEGFVREEEDRALPENEFKKQIWLLFEYPESSSPARGIAIVSVLVILISIVIFCLETLPEFRDDRDLIMALSAGGHSRLLNDTSAPHLENSGHTIFNDPFFIVETVCIVWFSFEFVVRCFACPSQALFFKNIMNIIDIVSILPYFITLGTDLAQQQGGGNGQQQQAMSFAILRIIRLVRVFRIFKLSRHSKGLQILGHTLRASMRELGLLIFFLFIGVILFSSAVYFAEADEPTTHFQSIPDAFWWAVVTMTTVGYGDMKPITVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETENEEQTQLTQNAVSCPYLPSNLLKKFRSSTSSSLGDKSEYLEMEEGVKESLCGKEEKCQGKGDDSETDKNNCSNAKAVETDV

Target ORF information:

RefSeq Version	XM_017591493.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium voltage-gated channel subfamily A member 4 (Kcna4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591493.1

ORF Insert Sequence:

ATGGAGGTGG CAATGGTGAG TGCCGAGAGC TCAGGGTGCA ACAGCCACAT GCCTTATGGT 
TATGCTGCCC AGGCCAGGGC TCGAGAGAGG GAGAGACTTG CTCACTCCAG GGCAGCTGCA 
GCTGCTGCTG TTGCAGCTGC CACGGCTGCG GTGGAAGGCA CTGGAGGTTC TGGTGGAGGC 
CCCCACCATC ATCATCAGAC ACGTGGGGCC TACTCCTCCC ATGATCCTCA AGGAAGCCGA 
GGTAGTCGGA GGAGGAGGCG ACAGCGAACT GAGAAGAAGA AACTCCACCA CAGGCAGAGC 
AGTTTTCCTC ATTGCTCAGA CCTGATGCCC AGTGGCTCTG AAGAGAAGAT CCTTAGGGAG 
CTGAGCGAGG AGGAGGAAGA CGAGGAGGAG GAAGAGGAGG AGGAAGAGGA GGGAAGGTTT 
TACTATAGTG AAGAGGACCA TGGGGATGGG TGTTCCTACA CTGACCTACT GCCACAGGAT 
GATGGGGGTG GCGGCGGCTA CAGTTCAGTC CGCTACAGTG ACTGTTGTGA ACGCGTGGTA 
ATAAATGTGT CTGGTCTACG CTTCGAAACC CAAATGAAAA CTTTGGCTCA GTTTCCAGAA 
ACTCTGTTGG GAGACCCTGA GAAGAGGACT CAGTACTTCG ACCCTTTGCG CAATGAGTAT 
TTTTTTGATA GGAACCGTCC CAGCTTTGAT GCCATTTTGT ATTATTACCA GTCAGGAGGC 
CGCCTGAAGA GGCCAGTCAA TGTCCCCTTT GATATCTTCA CTGAGGAGGT GAAGTTCTAT 
CAGTTGGGAG AGGAAGCCCT GCTCAAGTTC CGTGAGGATG AGGGCTTTGT GAGAGAAGAG 
GAGGACAGGG CTCTGCCAGA AAATGAATTT AAAAAACAGA TTTGGCTTCT CTTTGAATAT 
CCCGAGAGTT CCAGCCCTGC CAGGGGCATA GCCATCGTAT CTGTCCTGGT CATCTTAATC 
TCTATTGTCA TATTTTGCCT GGAAACCTTG CCTGAGTTCA GGGATGATAG GGACCTCATC 
ATGGCCCTCA GCGCAGGTGG ACACAGCAGA TTATTGAATG ACACCTCGGC ACCCCACCTG 
GAGAACTCAG GGCACACAAT ATTCAATGAC CCTTTCTTCA TTGTGGAGAC AGTATGTATC 
GTGTGGTTTT CCTTTGAGTT TGTGGTTCGA TGCTTTGCTT GTCCCAGTCA AGCACTCTTC 
TTCAAAAACA TCATGAACAT CATTGATATC GTCTCCATTT TGCCTTACTT CATCACTCTG 
GGCACCGATC TGGCCCAGCA GCAGGGGGGT GGCAACGGCC AGCAGCAGCA GGCTATGTCC 
TTTGCCATCC TCAGGATCAT CCGTCTGGTC CGAGTGTTCC GGATCTTCAA GCTCTCCAGA 
CACTCCAAGG GCCTGCAGAT CCTGGGCCAC ACCCTAAGAG CCAGCATGCG TGAACTGGGC 
CTTCTTATCT TTTTCCTCTT CATCGGGGTC ATCCTCTTTT CCAGCGCTGT GTATTTTGCA 
GAGGCAGATG AACCTACCAC CCATTTCCAA AGCATTCCAG ATGCGTTTTG GTGGGCTGTG 
GTAACCATGA CAACTGTGGG CTACGGGGAC ATGAAGCCCA TCACAGTGGG AGGAAAGATT 
GTGGGGTCCC TGTGTGCCAT TGCGGGTGTC TTAACCATTG CTTTGCCCGT GCCGGTGATT 
GTGTCTAACT TTAACTATTT CTACCACAGA GAGACTGAAA ACGAAGAACA GACCCAGCTG 
ACCCAAAACG CAGTCAGTTG CCCATACCTA CCTTCTAATT TGCTCAAGAA ATTTCGGAGC 
TCTACTTCTT CTTCCCTGGG GGACAAGTCA GAGTATCTAG AGATGGAAGA AGGGGTCAAG 
GAGTCTTTAT GTGGAAAGGA AGAGAAGTGT CAGGGAAAGG GGGATGACAG CGAGACAGAT 
AAAAACAACT GTTCTAATGC AAAGGCTGTG GAGACTGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Amino Acid Properties of Trafficking Determinants in the Outer Pore-Forming Region of Kv1 Potassium Channels in Cell Lines.
Cell biochemistry and biophysics75(1)25-33(2017 Mar)
Gomez B,Zhu J,Recio-Pinto E,Thornhill WB

Synaptotagmin I delays the fast inactivation of Kv1.4 channel through interaction with its N-terminus.
Molecular brain74(2014 Jan)
Xie C,Su H,Guo T,Yan Y,Peng X,Cao R,Wang Y,Chen P,Wang X,Liang S

Heme impairs the ball-and-chain inactivation of potassium channels.
Proceedings of the National Academy of Sciences of the United States of America110(42)E4036-44(2013 Oct)
Sahoo N,Goradia N,Ohlenschl?ger O,Sch?nherr R,Friedrich M,Plass W,Kappl R,Hoshi T,Heinemann SH

Hydrogen sulfide increases excitability through suppression of sustained potassium channel currents of rat trigeminal ganglion neurons.
Molecular pain94(2013 Feb)
Feng X,Zhou YL,Meng X,Qi FH,Chen W,Jiang X,Xu GY

KCNE1 and KCNE2 provide a checkpoint governing voltage-gated potassium channel ?-subunit composition.
Biophysical journal101(6)1364-75(2011 Sep)
Kanda VA,Lewis A,Xu X,Abbott GW

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Kcna4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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