Bcan cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bcan
Entrez Gene ID	25393
Full Name	brevican
Synonyms	ALPBRE,BEHAB
General protein information	Preferred Names brevican Names brevican core protein brain-enriched hyaluronan-binding protein brevican (brain specific proteoglycan in the aggrecan family)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	brain-specific member of the aggrecan family of chondroitin sulfate proteoglycans; extracellular matrix (ECM) protein; binds hyaluronan, a ubiquitous component of the ECM [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017590643.1	XP_017446132.1	brevican core protein isoform X1
XM_006232596.3	XP_006232658.1	brevican core protein isoform X1
NM_001033665.1	NP_001028837.1	brevican core protein isoform 1 precursor
NM_012916.2	NP_037048.2	brevican core protein isoform 2 precursor
XM_017590645.1	XP_017446134.1	brevican core protein isoform X1
XM_006232597.3	XP_006232659.1	brevican core protein isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	BCAN	XP_423655.4
Danio rerio (zebrafish)	bcan	NP_001077282.1
Canis lupus familiaris (dog)	BCAN	XP_005622754.1
Rattus norvegicus (Norway rat)	Bcan	NP_001028837.1
Homo sapiens (human)	BCAN	NP_068767.3
Pan troglodytes (chimpanzee)	BCAN	XP_001166305.1
	BCAN	XP_001116679.1
Xenopus tropicalis (tropical clawed frog)	LOC100486298	XP_002941551.2
Bos taurus (cattle)	BCAN	NP_776421.2
Mus musculus (house mouse)	Bcan	NP_031555.2

Gene Ontology
Bibliography

Related articles in PubMed

M?ller glial microRNAs are required for the maintenance of glial homeostasis and retinal architecture.
Wohl SG, Jorstad NL, Levine EM, Reh TA
Nature communications8(1)1603(2017 Nov)

Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
Favuzzi E, Marques-Smith A, Deogracias R, Winterflood CM, S?nchez-Aguilera A, Mantoan L, Maeso P, Fernandes C, Ewers H, Rico B
Neuron95(3)639-655.e10(2017 Aug)

Brevican knockdown reduces late-stage glioma tumor aggressiveness.
Dwyer CA, Bi WL, Viapiano MS, Matthews RT
Journal of neuro-oncology120(1)63-72(2014 Oct)

The role of brevican in glioma: promoting tumor cell motility in vitro and in vivo.
Lu R, Wu C, Guo L, Liu Y, Mo W, Wang H, Ding J, Wong ET, Yu M
BMC cancer12607(2012 Dec)

Alterations in protein regulators of neurodevelopment in the cerebrospinal fluid of infants with posthemorrhagic hydrocephalus of prematurity.
Morales DM, Townsend RR, Malone JP, Ewersmann CA, Macy EM, Inder TE, Limbrick DD Jr
Molecular & cellular proteomics : MCP11(6)M111.011973(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High Brevican expression is associated with glioma tumor aggressiveness.
Title: Brevican knockdown reduces late-stage glioma tumor aggressiveness.

Brevican deposition is elevated in response to progressive gray matter injury, whereas diminished versican expression may be associated with deep cerebral white matter injury.
Title: Versican and brevican are expressed with distinct pathology in neonatal hypoxic-ischemic injury.

Brevican is primarily synthesized by co-cultured GFAP-positive astrocytes and co-assembles with its interaction partners in perineuronal net-like structures on neuronal somata & neurites; axon initial segments are strongly covered by a dense brevican coat
Title: Brevican-containing perineuronal nets of extracellular matrix in dissociated hippocampal primary cultures.

TGFbeta may play a role in neuronal plasticity through its regulation of brevican and the activity of the ADAMTSs.
Title: Altered production and proteolytic processing of brevican by transforming growth factor beta in cultured astrocytes.

In a rodent model of neonatal hypoxia-ischemia, a marked decline is found in the expression of brevican, whose expression is correlated with oligodendrocyte survival, growth, and maturation.
Title: The effect of hypoxic-ischemic brain injury in perinatal rats on the abundance and proteolysis of brevican and NG2.

The following Bcan gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bcan cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01308	NM_012916.2 Latest version!	Rattus norvegicus brevican (Bcan), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa08518	NM_001033665.1 Latest version!	Rattus norvegicus brevican (Bcan), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa08518	XM_006232597.3 Latest version!	Rattus norvegicus brevican (Bcan), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa08518	XM_006232596.3 Latest version!	Rattus norvegicus brevican (Bcan), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa08518	XM_017590643.1 Latest version!	Rattus norvegicus brevican (Bcan), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa08518	XM_017590645.1 Latest version!	Rattus norvegicus brevican (Bcan), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bcan ( NM_012916.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037048.2 Rattus norvegicus brevican (Bcan), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa01308
Clone ID Related Accession (Same CDS sequence)	NM_012916.2
Accession Version	NM_012916.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	brevican core protein isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from Z28366.1, CB577797.1, X86406.1, AABR07072748.1 and AI535159.1. On Sep 21, 2005 this sequence version replaced NM_012916.1. Transcript Variant: This variant (2) lacks multiple exons in the 3' coding region and its 3' terminal exon extends past a splice site that is used in variant 1. The resulting protein (isoform 2) is shorter but has the same N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created using sequences from two different strains, Sprague-Dawley and BN/SsNHsdMCW, because no single transcript from the same strain was available for the full length of the gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X86406.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END##

ATGATCCCAT TGCTTCTGTC CCTGCTGGCA GCTCTGGTCC TGACCCAAGC CCCTGCAGCC 
CTCGCTGATG ACCTGAAAGA AGACAGCTCA GAGGATCGAG CCTTTCGGGT GCGCATCGGT 
GCCGCGCAGC TGCGGGGTGT GCTGGGCGGT GCCCTGGCCA TCCCATGCCA CGTCCACCAC 
CTGAGGCCGC CGCCCAGCCG CCGGGCCGCG CCGGGCTTTC CCCGAGTCAA ATGGACCTTC 
CTGTCCGGGG ACCGGGAGGT GGAGGTGCTG GTGGCGCGCG GGCTGCGCGT CAAGGTAAAC 
GAAGCCTATC GGTTCCGCGT GGCGCTGCCT GCCTACCCCG CATCGCTCAC AGATGTGTCT 
TTAGTATTGA GCGAACTGCG GCCCAATGAT TCCGGGGTCT ATCGCTGCGA GGTCCAGCAC 
GGTATCGACG ACAGCAGTGA TGCTGTGGAA GTCAAGGTCA AAGGGGTCGT CTTCCTCTAC 
CGAGAGGGCT CTGCCCGCTA TGCTTTCTCC TTCGCTGGAG CCCAGGAAGC CTGTGCTCGC 
ATCGGAGCCC GAATTGCCAC CCCTGAGCAG CTGTATGCTG CCTACCTCGG CGGCTATGAA 
CAGTGTGATG CTGGCTGGCT GTCCGACCAA ACCGTGAGGT ACCCCATCCA GAACCCACGA 
GAAGCCTGTT ATGGAGACAT GGATGGCTAC CCTGGAGTGC GGAATTACGG AGTGGTGGGT 
CCTGATGATC TCTACGATGT CTACTGTTAT GCCGAAGACC TAAATGGAGA ACTGTTCCTA 
GGTGCCCCTC CCGGCAAGCT GACGTGGGAG GAGGCTCGGG ACTACTGTCT GGAACGCGGT 
GCTCAGATCG CTAGCACGGG CCAGCTATAC GCGGCATGGA ATGGCGGCTT GGACAGATGT 
AGCCCTGGCT GGCTGGCTGA TGGCAGTGTG CGGTACCCCA TCATCACGCC CAGCCAACGC 
TGTGGGGGAG GCCTGCCAGG AGTCAAGACC CTCTTCCTCT TTCCCAACCA GACTGGCTTC 
CCCAGCAAGC AGAACCGCTT CAATGTCTAC TGCTTCCGAG ACTCTGCCCA TCCCTCTGCC 
TTCTCTGAGG CCTCCAGCCC AGCCTCTGAT GGACTAGAGG CCATTGTCAC AGTGACAGAG 
AAGCTGGAGG AACTGCAGTT GCCTCAGGAA GCTGTGGAGA GCGAGTCTCG TGGGGCGATC 
TACTCCATCC CCATCACAGA AGATGGGGGA GGAGGAAGCT CTACCCCAGA AGACCCAGCA 
GAGGCCCCCA GGACTCCTCT AGAATCAGAA ACCCAATCCG TTGCACCACC TACCGGGTCC 
TCAGAAGAGG AAGGCGAAGC CCTGGAGGAA GAAGAAAGAT TCAAAGACAC AGAGACTCCG 
AAGGAAGAGA AGGAGCAGGA GAACCTGTGG GTGTGGCCCA CGGAGCTCAG CAGCCCTCTC 
CCTACTGGCT TGGAAACAGA GCACTCACTC TCCCAGGTGT CCCCACCAGC CCAGGCAGTT 
CTACAGCTGG GTGCATCACC TTCTCCCAGG CCTCCAAGGG TCCATGGACC GCCTGCAGAG 
ACTTTGCAAC CCCCAAGGGA GGGAAGCCTC ACATCTACTC CAGATGGGGC AAGAGAAGTA 
GCGGGGGAAA CTGGGAGCCC TGAGCTCTCT GGGGTTCCTC GAGAAAGCGA GGAGGCAGGA 
AGCTCCAGCT TGGAGGATGG CCCTTCCCTC CTTCCAGCGA CATGGGCCCC TGTGGGTACC 
AGGGAGCTGG AGACCCCCTC AGAAGAGAAG TCTGGAAGAA CTGTTCTGAC AGGCACATCA 
GTGCAGGCCC AGCCAGTGCT GCCCACCGAC AGTGCCAGCC GAGGTGGAGT GGCTGTGGCT 
CCCTCATCAG GTAATTCTGC CGAAGGCTCG ATGCCCGCCT TTCTCCTTTT CCTCCTCCTC 
CAGCTCTGGG CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037048.2
CDS	256..2193
Translation	MIPLLLSLLAALVLTQAPAALADDLKEDSSEDRAFRVRIGAAQLRGVLGGALAIPCHVHHLRPPPSRRAAPGFPRVKWTFLSGDREVEVLVARGLRVKVNEAYRFRVALPAYPASLTDVSLVLSELRPNDSGVYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFAGAQEACARIGARIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQNPREACYGDMDGYPGVRNYGVVGPDDLYDVYCYAEDLNGELFLGAPPGKLTWEEARDYCLERGAQIASTGQLYAAWNGGLDRCSPGWLADGSVRYPIITPSQRCGGGLPGVKTLFLFPNQTGFPSKQNRFNVYCFRDSAHPSAFSEASSPASDGLEAIVTVTEKLEELQLPQEAVESESRGAIYSIPITEDGGGGSSTPEDPAEAPRTPLESETQSVAPPTGSSEEEGEALEEEERFKDTETPKEEKEQENLWVWPTELSSPLPTGLETEHSLSQVSPPAQAVLQLGASPSPRPPRVHGPPAETLQPPREGSLTSTPDGAREVAGETGSPELSGVPRESEEAGSSSLEDGPSLLPATWAPVGTRELETPSEEKSGRTVLTGTSVQAQPVLPTDSASRGGVAVAPSSGNSAEGSMPAFLLFLLLQLWAT

Target ORF information:

RefSeq Version	NM_012916.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus brevican (Bcan), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012916.2

ORF Insert Sequence:

ATGATCCCAT TGCTTCTGTC CCTGCTGGCA GCTCTGGTCC TGACCCAAGC CCCTGCAGCC 
CTCGCTGATG ACCTGAAAGA AGACAGCTCA GAGGATCGAG CCTTTCGGGT GCGCATCGGT 
GCCGCGCAGC TGCGGGGTGT GCTGGGCGGT GCCCTGGCCA TCCCATGCCA CGTCCACCAC 
CTGAGGCCGC CGCCCAGCCG CCGGGCCGCG CCGGGCTTTC CCCGAGTCAA ATGGACCTTC 
CTGTCCGGGG ACCGGGAGGT GGAGGTGCTG GTGGCGCGCG GGCTGCGCGT CAAGGTAAAC 
GAAGCCTATC GGTTCCGCGT GGCGCTGCCT GCCTACCCCG CATCGCTCAC AGATGTGTCT 
TTAGTATTGA GCGAACTGCG GCCCAATGAT TCCGGGGTCT ATCGCTGCGA GGTCCAGCAC 
GGTATCGACG ACAGCAGTGA TGCTGTGGAA GTCAAGGTCA AAGGGGTCGT CTTCCTCTAC 
CGAGAGGGCT CTGCCCGCTA TGCTTTCTCC TTCGCTGGAG CCCAGGAAGC CTGTGCTCGC 
ATCGGAGCCC GAATTGCCAC CCCTGAGCAG CTGTATGCTG CCTACCTCGG CGGCTATGAA 
CAGTGTGATG CTGGCTGGCT GTCCGACCAA ACCGTGAGGT ACCCCATCCA GAACCCACGA 
GAAGCCTGTT ATGGAGACAT GGATGGCTAC CCTGGAGTGC GGAATTACGG AGTGGTGGGT 
CCTGATGATC TCTACGATGT CTACTGTTAT GCCGAAGACC TAAATGGAGA ACTGTTCCTA 
GGTGCCCCTC CCGGCAAGCT GACGTGGGAG GAGGCTCGGG ACTACTGTCT GGAACGCGGT 
GCTCAGATCG CTAGCACGGG CCAGCTATAC GCGGCATGGA ATGGCGGCTT GGACAGATGT 
AGCCCTGGCT GGCTGGCTGA TGGCAGTGTG CGGTACCCCA TCATCACGCC CAGCCAACGC 
TGTGGGGGAG GCCTGCCAGG AGTCAAGACC CTCTTCCTCT TTCCCAACCA GACTGGCTTC 
CCCAGCAAGC AGAACCGCTT CAATGTCTAC TGCTTCCGAG ACTCTGCCCA TCCCTCTGCC 
TTCTCTGAGG CCTCCAGCCC AGCCTCTGAT GGACTAGAGG CCATTGTCAC AGTGACAGAG 
AAGCTGGAGG AACTGCAGTT GCCTCAGGAA GCTGTGGAGA GCGAGTCTCG TGGGGCGATC 
TACTCCATCC CCATCACAGA AGATGGGGGA GGAGGAAGCT CTACCCCAGA AGACCCAGCA 
GAGGCCCCCA GGACTCCTCT AGAATCAGAA ACCCAATCCG TTGCACCACC TACCGGGTCC 
TCAGAAGAGG AAGGCGAAGC CCTGGAGGAA GAAGAAAGAT TCAAAGACAC AGAGACTCCG 
AAGGAAGAGA AGGAGCAGGA GAACCTGTGG GTGTGGCCCA CGGAGCTCAG CAGCCCTCTC 
CCTACTGGCT TGGAAACAGA GCACTCACTC TCCCAGGTGT CCCCACCAGC CCAGGCAGTT 
CTACAGCTGG GTGCATCACC TTCTCCCAGG CCTCCAAGGG TCCATGGACC GCCTGCAGAG 
ACTTTGCAAC CCCCAAGGGA GGGAAGCCTC ACATCTACTC CAGATGGGGC AAGAGAAGTA 
GCGGGGGAAA CTGGGAGCCC TGAGCTCTCT GGGGTTCCTC GAGAAAGCGA GGAGGCAGGA 
AGCTCCAGCT TGGAGGATGG CCCTTCCCTC CTTCCAGCGA CATGGGCCCC TGTGGGTACC 
AGGGAGCTGG AGACCCCCTC AGAAGAGAAG TCTGGAAGAA CTGTTCTGAC AGGCACATCA 
GTGCAGGCCC AGCCAGTGCT GCCCACCGAC AGTGCCAGCC GAGGTGGAGT GGCTGTGGCT 
CCCTCATCAG GTAATTCTGC CGAAGGCTCG ATGCCCGCCT TTCTCCTTTT CCTCCTCCTC 
CAGCTCTGGG CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bcan ( NM_001033665.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001028837.1 Rattus norvegicus brevican (Bcan), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa08518
Clone ID Related Accession (Same CDS sequence)	XM_017590643.1 , XM_006232596.3 , NM_001033665.1 , XM_017590645.1 , XM_006232597.3
Accession Version	NM_001033665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	brevican core protein isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from Z28366.1, CB577797.1, U37142.1, CB784255.1 and CB810773.1. On Sep 22, 2005 this sequence version replaced XM_579358.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U37142.1, X79881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689595, SAMN02642596 [ECO:0000348] ##Evidence-Data-END##

ATGATCCCAT TGCTTCTGTC CCTGCTGGCA GCTCTGGTCC TGACCCAAGC CCCTGCAGCC 
CTCGCTGATG ACCTGAAAGA AGACAGCTCA GAGGATCGAG CCTTTCGGGT GCGCATCGGT 
GCCGCGCAGC TGCGGGGTGT GCTGGGCGGT GCCCTGGCCA TCCCATGCCA CGTCCACCAC 
CTGAGGCCGC CGCCCAGCCG CCGGGCCGCG CCGGGCTTTC CCCGAGTCAA ATGGACCTTC 
CTGTCCGGGG ACCGGGAGGT GGAGGTGCTG GTGGCGCGCG GGCTGCGCGT CAAGGTAAAC 
GAAGCCTATC GGTTCCGCGT GGCGCTGCCT GCCTACCCCG CATCGCTCAC AGATGTGTCT 
TTAGTATTGA GCGAACTGCG GCCCAATGAT TCCGGGGTCT ATCGCTGCGA GGTCCAGCAC 
GGTATCGACG ACAGCAGTGA TGCTGTGGAA GTCAAGGTCA AAGGGGTCGT CTTCCTCTAC 
CGAGAGGGCT CTGCCCGCTA TGCTTTCTCC TTCGCTGGAG CCCAGGAAGC CTGTGCTCGC 
ATCGGAGCCC GAATTGCCAC CCCTGAGCAG CTGTATGCTG CCTACCTCGG CGGCTATGAA 
CAGTGTGATG CTGGCTGGCT GTCCGACCAA ACCGTGAGGT ACCCCATCCA GAACCCACGA 
GAAGCCTGTT ATGGAGACAT GGATGGCTAC CCTGGAGTGC GGAATTACGG AGTGGTGGGT 
CCTGATGATC TCTACGATGT CTACTGTTAT GCCGAAGACC TAAATGGAGA ACTGTTCCTA 
GGTGCCCCTC CCGGCAAGCT GACGTGGGAG GAGGCTCGGG ACTACTGTCT GGAACGCGGT 
GCTCAGATCG CTAGCACGGG CCAGCTATAC GCGGCATGGA ATGGCGGCTT GGACAGATGT 
AGCCCTGGCT GGCTGGCTGA TGGCAGTGTG CGGTACCCCA TCATCACGCC CAGCCAACGC 
TGTGGGGGAG GCCTGCCAGG AGTCAAGACC CTCTTCCTCT TTCCCAACCA GACTGGCTTC 
CCCAGCAAGC AGAACCGCTT CAATGTCTAC TGCTTCCGAG ACTCTGCCCA TCCCTCTGCC 
TTCTCTGAGG CCTCCAGCCC AGCCTCTGAT GGACTAGAGG CCATTGTCAC AGTGACAGAG 
AAGCTGGAGG AACTGCAGTT GCCTCAGGAA GCTGTGGAGA GCGAGTCTCG TGGGGCGATC 
TACTCCATCC CCATCACAGA AGATGGGGGA GGAGGAAGCT CTACCCCAGA AGACCCAGCA 
GAGGCCCCCA GGACTCCTCT AGAATCAGAA ACCCAATCCG TTGCACCACC TACCGGGTCC 
TCAGAAGAGG AAGGCGAAGC CCTGGAGGAA GAAGAAAGAT TCAAAGACAC AGAGACTCCG 
AAGGAAGAGA AGGAGCAGGA GAACCTGTGG GTGTGGCCCA CGGAGCTCAG CAGCCCTCTC 
CCTACTGGCT TGGAAACAGA GCACTCACTC TCCCAGGTGT CCCCACCAGC CCAGGCAGTT 
CTACAGCTGG GTGCATCACC TTCTCCCAGG CCTCCAAGGG TCCATGGACC GCCTGCAGAG 
ACTTTGCAAC CCCCAAGGGA GGGAAGCCTC ACATCTACTC CAGATGGGGC AAGAGAAGTA 
GCGGGGGAAA CTGGGAGCCC TGAGCTCTCT GGGGTTCCTC GAGAAAGCGA GGAGGCAGGA 
AGCTCCAGCT TGGAGGATGG CCCTTCCCTC CTTCCAGCGA CATGGGCCCC TGTGGGTACC 
AGGGAGCTGG AGACCCCCTC AGAAGAGAAG TCTGGAAGAA CTGTTCTGAC AGGCACATCA 
GTGCAGGCCC AGCCAGTGCT GCCCACCGAC AGTGCCAGCC GAGGTGGAGT GGCTGTGGCT 
CCCTCATCAG GTGACTGTAT CCCCAGCCCC TGCCACAATG GTGGGACATG CTTGGAGGAG 
AAGGAGGGTT TCCGCTGCCT CTGTTTGCCA GGCTATGGGG GGGACCTGTG CGATGTTGGC 
CTCCACTTCT GCAGCCCGGG CTGGGAGGCC TTCCAGGGTG CCTGCTACAA GCACTTTTCC 
ACACGAAGGA GTTGGGAGGA GGCAGAAAGC CAGTGCCGAG CGCTAGGGGC TCATCTGACC 
AGCATCTGCA CCCCTGAGGA GCAGGACTTT GTCAACGATC GATACAGGGA GTACCAGTGG 
ATTGGGCTCA ATGACAGGAC CATCGAGGGT GACTTCCTGT GGTCAGATGG TGCCCCTCTG 
CTCTATGAAA ACTGGAACCC TGGGCAGCCT GACAGCTACT TCCTGTCTGG GGAGAACTGT 
GTGGTCATGG TGTGGCATGA CCAGGGACAG TGGAGTGATG TACCCTGCAA CTACCACCTA 
TCCTACACCT GCAAGATGGG GCTTGTGTCA TGTGGACCTC CACCACAGCT GCCCCTGGCT 
CAAATATTTG GTCGCCCTCG GCTGCGCTAC GCGGTGGACA CTGTGCTTCG ATATCGGTGC 
CGAGACGGGC TGGCCCAGCG CAACTTGCCG TTGATCCGCT GCCAGGAGAA TGGGCTTTGG 
GAGGCCCCTC AGATTTCTTG CGTGCCCCGA AGACCTGCCC GTGCTCTCCG CTCAATGACC 
GCCCCAGAAG GACCACGGGG ACAGCTCCCG AGGCAGAGGA AAGCACTGTT GACACCTCCC 
TCCAGTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028837.1
CDS	256..2907
Translation	MIPLLLSLLAALVLTQAPAALADDLKEDSSEDRAFRVRIGAAQLRGVLGGALAIPCHVHHLRPPPSRRAAPGFPRVKWTFLSGDREVEVLVARGLRVKVNEAYRFRVALPAYPASLTDVSLVLSELRPNDSGVYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFAGAQEACARIGARIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQNPREACYGDMDGYPGVRNYGVVGPDDLYDVYCYAEDLNGELFLGAPPGKLTWEEARDYCLERGAQIASTGQLYAAWNGGLDRCSPGWLADGSVRYPIITPSQRCGGGLPGVKTLFLFPNQTGFPSKQNRFNVYCFRDSAHPSAFSEASSPASDGLEAIVTVTEKLEELQLPQEAVESESRGAIYSIPITEDGGGGSSTPEDPAEAPRTPLESETQSVAPPTGSSEEEGEALEEEERFKDTETPKEEKEQENLWVWPTELSSPLPTGLETEHSLSQVSPPAQAVLQLGASPSPRPPRVHGPPAETLQPPREGSLTSTPDGAREVAGETGSPELSGVPRESEEAGSSSLEDGPSLLPATWAPVGTRELETPSEEKSGRTVLTGTSVQAQPVLPTDSASRGGVAVAPSSGDCIPSPCHNGGTCLEEKEGFRCLCLPGYGGDLCDVGLHFCSPGWEAFQGACYKHFSTRRSWEEAESQCRALGAHLTSICTPEEQDFVNDRYREYQWIGLNDRTIEGDFLWSDGAPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQWSDVPCNYHLSYTCKMGLVSCGPPPQLPLAQIFGRPRLRYAVDTVLRYRCRDGLAQRNLPLIRCQENGLWEAPQISCVPRRPARALRSMTAPEGPRGQLPRQRKALLTPPSSL

Target ORF information:

RefSeq Version	NM_001033665.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus brevican (Bcan), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033665.1

ORF Insert Sequence:

ATGATCCCAT TGCTTCTGTC CCTGCTGGCA GCTCTGGTCC TGACCCAAGC CCCTGCAGCC 
CTCGCTGATG ACCTGAAAGA AGACAGCTCA GAGGATCGAG CCTTTCGGGT GCGCATCGGT 
GCCGCGCAGC TGCGGGGTGT GCTGGGCGGT GCCCTGGCCA TCCCATGCCA CGTCCACCAC 
CTGAGGCCGC CGCCCAGCCG CCGGGCCGCG CCGGGCTTTC CCCGAGTCAA ATGGACCTTC 
CTGTCCGGGG ACCGGGAGGT GGAGGTGCTG GTGGCGCGCG GGCTGCGCGT CAAGGTAAAC 
GAAGCCTATC GGTTCCGCGT GGCGCTGCCT GCCTACCCCG CATCGCTCAC AGATGTGTCT 
TTAGTATTGA GCGAACTGCG GCCCAATGAT TCCGGGGTCT ATCGCTGCGA GGTCCAGCAC 
GGTATCGACG ACAGCAGTGA TGCTGTGGAA GTCAAGGTCA AAGGGGTCGT CTTCCTCTAC 
CGAGAGGGCT CTGCCCGCTA TGCTTTCTCC TTCGCTGGAG CCCAGGAAGC CTGTGCTCGC 
ATCGGAGCCC GAATTGCCAC CCCTGAGCAG CTGTATGCTG CCTACCTCGG CGGCTATGAA 
CAGTGTGATG CTGGCTGGCT GTCCGACCAA ACCGTGAGGT ACCCCATCCA GAACCCACGA 
GAAGCCTGTT ATGGAGACAT GGATGGCTAC CCTGGAGTGC GGAATTACGG AGTGGTGGGT 
CCTGATGATC TCTACGATGT CTACTGTTAT GCCGAAGACC TAAATGGAGA ACTGTTCCTA 
GGTGCCCCTC CCGGCAAGCT GACGTGGGAG GAGGCTCGGG ACTACTGTCT GGAACGCGGT 
GCTCAGATCG CTAGCACGGG CCAGCTATAC GCGGCATGGA ATGGCGGCTT GGACAGATGT 
AGCCCTGGCT GGCTGGCTGA TGGCAGTGTG CGGTACCCCA TCATCACGCC CAGCCAACGC 
TGTGGGGGAG GCCTGCCAGG AGTCAAGACC CTCTTCCTCT TTCCCAACCA GACTGGCTTC 
CCCAGCAAGC AGAACCGCTT CAATGTCTAC TGCTTCCGAG ACTCTGCCCA TCCCTCTGCC 
TTCTCTGAGG CCTCCAGCCC AGCCTCTGAT GGACTAGAGG CCATTGTCAC AGTGACAGAG 
AAGCTGGAGG AACTGCAGTT GCCTCAGGAA GCTGTGGAGA GCGAGTCTCG TGGGGCGATC 
TACTCCATCC CCATCACAGA AGATGGGGGA GGAGGAAGCT CTACCCCAGA AGACCCAGCA 
GAGGCCCCCA GGACTCCTCT AGAATCAGAA ACCCAATCCG TTGCACCACC TACCGGGTCC 
TCAGAAGAGG AAGGCGAAGC CCTGGAGGAA GAAGAAAGAT TCAAAGACAC AGAGACTCCG 
AAGGAAGAGA AGGAGCAGGA GAACCTGTGG GTGTGGCCCA CGGAGCTCAG CAGCCCTCTC 
CCTACTGGCT TGGAAACAGA GCACTCACTC TCCCAGGTGT CCCCACCAGC CCAGGCAGTT 
CTACAGCTGG GTGCATCACC TTCTCCCAGG CCTCCAAGGG TCCATGGACC GCCTGCAGAG 
ACTTTGCAAC CCCCAAGGGA GGGAAGCCTC ACATCTACTC CAGATGGGGC AAGAGAAGTA 
GCGGGGGAAA CTGGGAGCCC TGAGCTCTCT GGGGTTCCTC GAGAAAGCGA GGAGGCAGGA 
AGCTCCAGCT TGGAGGATGG CCCTTCCCTC CTTCCAGCGA CATGGGCCCC TGTGGGTACC 
AGGGAGCTGG AGACCCCCTC AGAAGAGAAG TCTGGAAGAA CTGTTCTGAC AGGCACATCA 
GTGCAGGCCC AGCCAGTGCT GCCCACCGAC AGTGCCAGCC GAGGTGGAGT GGCTGTGGCT 
CCCTCATCAG GTGACTGTAT CCCCAGCCCC TGCCACAATG GTGGGACATG CTTGGAGGAG 
AAGGAGGGTT TCCGCTGCCT CTGTTTGCCA GGCTATGGGG GGGACCTGTG CGATGTTGGC 
CTCCACTTCT GCAGCCCGGG CTGGGAGGCC TTCCAGGGTG CCTGCTACAA GCACTTTTCC 
ACACGAAGGA GTTGGGAGGA GGCAGAAAGC CAGTGCCGAG CGCTAGGGGC TCATCTGACC 
AGCATCTGCA CCCCTGAGGA GCAGGACTTT GTCAACGATC GATACAGGGA GTACCAGTGG 
ATTGGGCTCA ATGACAGGAC CATCGAGGGT GACTTCCTGT GGTCAGATGG TGCCCCTCTG 
CTCTATGAAA ACTGGAACCC TGGGCAGCCT GACAGCTACT TCCTGTCTGG GGAGAACTGT 
GTGGTCATGG TGTGGCATGA CCAGGGACAG TGGAGTGATG TACCCTGCAA CTACCACCTA 
TCCTACACCT GCAAGATGGG GCTTGTGTCA TGTGGACCTC CACCACAGCT GCCCCTGGCT 
CAAATATTTG GTCGCCCTCG GCTGCGCTAC GCGGTGGACA CTGTGCTTCG ATATCGGTGC 
CGAGACGGGC TGGCCCAGCG CAACTTGCCG TTGATCCGCT GCCAGGAGAA TGGGCTTTGG 
GAGGCCCCTC AGATTTCTTG CGTGCCCCGA AGACCTGCCC GTGCTCTCCG CTCAATGACC 
GCCCCAGAAG GACCACGGGG ACAGCTCCCG AGGCAGAGGA AAGCACTGTT GACACCTCCC 
TCCAGTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bcan ( XM_017590643.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446132.1 Rattus norvegicus brevican (Bcan), transcript variant X2, mRNA.

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CloneID	ORa08518
Clone ID Related Accession (Same CDS sequence)	XM_017590643.1 , XM_006232596.3 , NM_001033665.1 , XM_017590645.1 , XM_006232597.3
Accession Version	XM_017590643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	brevican core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCCAT TGCTTCTGTC CCTGCTGGCA GCTCTGGTCC TGACCCAAGC CCCTGCAGCC 
CTCGCTGATG ACCTGAAAGA AGACAGCTCA GAGGATCGAG CCTTTCGGGT GCGCATCGGT 
GCCGCGCAGC TGCGGGGTGT GCTGGGCGGT GCCCTGGCCA TCCCATGCCA CGTCCACCAC 
CTGAGGCCGC CGCCCAGCCG CCGGGCCGCG CCGGGCTTTC CCCGAGTCAA ATGGACCTTC 
CTGTCCGGGG ACCGGGAGGT GGAGGTGCTG GTGGCGCGCG GGCTGCGCGT CAAGGTAAAC 
GAAGCCTATC GGTTCCGCGT GGCGCTGCCT GCCTACCCCG CATCGCTCAC AGATGTGTCT 
TTAGTATTGA GCGAACTGCG GCCCAATGAT TCCGGGGTCT ATCGCTGCGA GGTCCAGCAC 
GGTATCGACG ACAGCAGTGA TGCTGTGGAA GTCAAGGTCA AAGGGGTCGT CTTCCTCTAC 
CGAGAGGGCT CTGCCCGCTA TGCTTTCTCC TTCGCTGGAG CCCAGGAAGC CTGTGCTCGC 
ATCGGAGCCC GAATTGCCAC CCCTGAGCAG CTGTATGCTG CCTACCTCGG CGGCTATGAA 
CAGTGTGATG CTGGCTGGCT GTCCGACCAA ACCGTGAGGT ACCCCATCCA GAACCCACGA 
GAAGCCTGTT ATGGAGACAT GGATGGCTAC CCTGGAGTGC GGAATTACGG AGTGGTGGGT 
CCTGATGATC TCTACGATGT CTACTGTTAT GCCGAAGACC TAAATGGAGA ACTGTTCCTA 
GGTGCCCCTC CCGGCAAGCT GACGTGGGAG GAGGCTCGGG ACTACTGTCT GGAACGCGGT 
GCTCAGATCG CTAGCACGGG CCAGCTATAC GCGGCATGGA ATGGCGGCTT GGACAGATGT 
AGCCCTGGCT GGCTGGCTGA TGGCAGTGTG CGGTACCCCA TCATCACGCC CAGCCAACGC 
TGTGGGGGAG GCCTGCCAGG AGTCAAGACC CTCTTCCTCT TTCCCAACCA GACTGGCTTC 
CCCAGCAAGC AGAACCGCTT CAATGTCTAC TGCTTCCGAG ACTCTGCCCA TCCCTCTGCC 
TTCTCTGAGG CCTCCAGCCC AGCCTCTGAT GGACTAGAGG CCATTGTCAC AGTGACAGAG 
AAGCTGGAGG AACTGCAGTT GCCTCAGGAA GCTGTGGAGA GCGAGTCTCG TGGGGCGATC 
TACTCCATCC CCATCACAGA AGATGGGGGA GGAGGAAGCT CTACCCCAGA AGACCCAGCA 
GAGGCCCCCA GGACTCCTCT AGAATCAGAA ACCCAATCCG TTGCACCACC TACCGGGTCC 
TCAGAAGAGG AAGGCGAAGC CCTGGAGGAA GAAGAAAGAT TCAAAGACAC AGAGACTCCG 
AAGGAAGAGA AGGAGCAGGA GAACCTGTGG GTGTGGCCCA CGGAGCTCAG CAGCCCTCTC 
CCTACTGGCT TGGAAACAGA GCACTCACTC TCCCAGGTGT CCCCACCAGC CCAGGCAGTT 
CTACAGCTGG GTGCATCACC TTCTCCCAGG CCTCCAAGGG TCCATGGACC GCCTGCAGAG 
ACTTTGCAAC CCCCAAGGGA GGGAAGCCTC ACATCTACTC CAGATGGGGC AAGAGAAGTA 
GCGGGGGAAA CTGGGAGCCC TGAGCTCTCT GGGGTTCCTC GAGAAAGCGA GGAGGCAGGA 
AGCTCCAGCT TGGAGGATGG CCCTTCCCTC CTTCCAGCGA CATGGGCCCC TGTGGGTACC 
AGGGAGCTGG AGACCCCCTC AGAAGAGAAG TCTGGAAGAA CTGTTCTGAC AGGCACATCA 
GTGCAGGCCC AGCCAGTGCT GCCCACCGAC AGTGCCAGCC GAGGTGGAGT GGCTGTGGCT 
CCCTCATCAG GTGACTGTAT CCCCAGCCCC TGCCACAATG GTGGGACATG CTTGGAGGAG 
AAGGAGGGTT TCCGCTGCCT CTGTTTGCCA GGCTATGGGG GGGACCTGTG CGATGTTGGC 
CTCCACTTCT GCAGCCCGGG CTGGGAGGCC TTCCAGGGTG CCTGCTACAA GCACTTTTCC 
ACACGAAGGA GTTGGGAGGA GGCAGAAAGC CAGTGCCGAG CGCTAGGGGC TCATCTGACC 
AGCATCTGCA CCCCTGAGGA GCAGGACTTT GTCAACGATC GATACAGGGA GTACCAGTGG 
ATTGGGCTCA ATGACAGGAC CATCGAGGGT GACTTCCTGT GGTCAGATGG TGCCCCTCTG 
CTCTATGAAA ACTGGAACCC TGGGCAGCCT GACAGCTACT TCCTGTCTGG GGAGAACTGT 
GTGGTCATGG TGTGGCATGA CCAGGGACAG TGGAGTGATG TACCCTGCAA CTACCACCTA 
TCCTACACCT GCAAGATGGG GCTTGTGTCA TGTGGACCTC CACCACAGCT GCCCCTGGCT 
CAAATATTTG GTCGCCCTCG GCTGCGCTAC GCGGTGGACA CTGTGCTTCG ATATCGGTGC 
CGAGACGGGC TGGCCCAGCG CAACTTGCCG TTGATCCGCT GCCAGGAGAA TGGGCTTTGG 
GAGGCCCCTC AGATTTCTTG CGTGCCCCGA AGACCTGCCC GTGCTCTCCG CTCAATGACC 
GCCCCAGAAG GACCACGGGG ACAGCTCCCG AGGCAGAGGA AAGCACTGTT GACACCTCCC 
TCCAGTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446132.1
CDS	109..2760
Translation	MIPLLLSLLAALVLTQAPAALADDLKEDSSEDRAFRVRIGAAQLRGVLGGALAIPCHVHHLRPPPSRRAAPGFPRVKWTFLSGDREVEVLVARGLRVKVNEAYRFRVALPAYPASLTDVSLVLSELRPNDSGVYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFAGAQEACARIGARIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQNPREACYGDMDGYPGVRNYGVVGPDDLYDVYCYAEDLNGELFLGAPPGKLTWEEARDYCLERGAQIASTGQLYAAWNGGLDRCSPGWLADGSVRYPIITPSQRCGGGLPGVKTLFLFPNQTGFPSKQNRFNVYCFRDSAHPSAFSEASSPASDGLEAIVTVTEKLEELQLPQEAVESESRGAIYSIPITEDGGGGSSTPEDPAEAPRTPLESETQSVAPPTGSSEEEGEALEEEERFKDTETPKEEKEQENLWVWPTELSSPLPTGLETEHSLSQVSPPAQAVLQLGASPSPRPPRVHGPPAETLQPPREGSLTSTPDGAREVAGETGSPELSGVPRESEEAGSSSLEDGPSLLPATWAPVGTRELETPSEEKSGRTVLTGTSVQAQPVLPTDSASRGGVAVAPSSGDCIPSPCHNGGTCLEEKEGFRCLCLPGYGGDLCDVGLHFCSPGWEAFQGACYKHFSTRRSWEEAESQCRALGAHLTSICTPEEQDFVNDRYREYQWIGLNDRTIEGDFLWSDGAPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQWSDVPCNYHLSYTCKMGLVSCGPPPQLPLAQIFGRPRLRYAVDTVLRYRCRDGLAQRNLPLIRCQENGLWEAPQISCVPRRPARALRSMTAPEGPRGQLPRQRKALLTPPSSL

Target ORF information:

RefSeq Version	XM_017590643.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus brevican (Bcan), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590643.1

ORF Insert Sequence:

ATGATCCCAT TGCTTCTGTC CCTGCTGGCA GCTCTGGTCC TGACCCAAGC CCCTGCAGCC 
CTCGCTGATG ACCTGAAAGA AGACAGCTCA GAGGATCGAG CCTTTCGGGT GCGCATCGGT 
GCCGCGCAGC TGCGGGGTGT GCTGGGCGGT GCCCTGGCCA TCCCATGCCA CGTCCACCAC 
CTGAGGCCGC CGCCCAGCCG CCGGGCCGCG CCGGGCTTTC CCCGAGTCAA ATGGACCTTC 
CTGTCCGGGG ACCGGGAGGT GGAGGTGCTG GTGGCGCGCG GGCTGCGCGT CAAGGTAAAC 
GAAGCCTATC GGTTCCGCGT GGCGCTGCCT GCCTACCCCG CATCGCTCAC AGATGTGTCT 
TTAGTATTGA GCGAACTGCG GCCCAATGAT TCCGGGGTCT ATCGCTGCGA GGTCCAGCAC 
GGTATCGACG ACAGCAGTGA TGCTGTGGAA GTCAAGGTCA AAGGGGTCGT CTTCCTCTAC 
CGAGAGGGCT CTGCCCGCTA TGCTTTCTCC TTCGCTGGAG CCCAGGAAGC CTGTGCTCGC 
ATCGGAGCCC GAATTGCCAC CCCTGAGCAG CTGTATGCTG CCTACCTCGG CGGCTATGAA 
CAGTGTGATG CTGGCTGGCT GTCCGACCAA ACCGTGAGGT ACCCCATCCA GAACCCACGA 
GAAGCCTGTT ATGGAGACAT GGATGGCTAC CCTGGAGTGC GGAATTACGG AGTGGTGGGT 
CCTGATGATC TCTACGATGT CTACTGTTAT GCCGAAGACC TAAATGGAGA ACTGTTCCTA 
GGTGCCCCTC CCGGCAAGCT GACGTGGGAG GAGGCTCGGG ACTACTGTCT GGAACGCGGT 
GCTCAGATCG CTAGCACGGG CCAGCTATAC GCGGCATGGA ATGGCGGCTT GGACAGATGT 
AGCCCTGGCT GGCTGGCTGA TGGCAGTGTG CGGTACCCCA TCATCACGCC CAGCCAACGC 
TGTGGGGGAG GCCTGCCAGG AGTCAAGACC CTCTTCCTCT TTCCCAACCA GACTGGCTTC 
CCCAGCAAGC AGAACCGCTT CAATGTCTAC TGCTTCCGAG ACTCTGCCCA TCCCTCTGCC 
TTCTCTGAGG CCTCCAGCCC AGCCTCTGAT GGACTAGAGG CCATTGTCAC AGTGACAGAG 
AAGCTGGAGG AACTGCAGTT GCCTCAGGAA GCTGTGGAGA GCGAGTCTCG TGGGGCGATC 
TACTCCATCC CCATCACAGA AGATGGGGGA GGAGGAAGCT CTACCCCAGA AGACCCAGCA 
GAGGCCCCCA GGACTCCTCT AGAATCAGAA ACCCAATCCG TTGCACCACC TACCGGGTCC 
TCAGAAGAGG AAGGCGAAGC CCTGGAGGAA GAAGAAAGAT TCAAAGACAC AGAGACTCCG 
AAGGAAGAGA AGGAGCAGGA GAACCTGTGG GTGTGGCCCA CGGAGCTCAG CAGCCCTCTC 
CCTACTGGCT TGGAAACAGA GCACTCACTC TCCCAGGTGT CCCCACCAGC CCAGGCAGTT 
CTACAGCTGG GTGCATCACC TTCTCCCAGG CCTCCAAGGG TCCATGGACC GCCTGCAGAG 
ACTTTGCAAC CCCCAAGGGA GGGAAGCCTC ACATCTACTC CAGATGGGGC AAGAGAAGTA 
GCGGGGGAAA CTGGGAGCCC TGAGCTCTCT GGGGTTCCTC GAGAAAGCGA GGAGGCAGGA 
AGCTCCAGCT TGGAGGATGG CCCTTCCCTC CTTCCAGCGA CATGGGCCCC TGTGGGTACC 
AGGGAGCTGG AGACCCCCTC AGAAGAGAAG TCTGGAAGAA CTGTTCTGAC AGGCACATCA 
GTGCAGGCCC AGCCAGTGCT GCCCACCGAC AGTGCCAGCC GAGGTGGAGT GGCTGTGGCT 
CCCTCATCAG GTGACTGTAT CCCCAGCCCC TGCCACAATG GTGGGACATG CTTGGAGGAG 
AAGGAGGGTT TCCGCTGCCT CTGTTTGCCA GGCTATGGGG GGGACCTGTG CGATGTTGGC 
CTCCACTTCT GCAGCCCGGG CTGGGAGGCC TTCCAGGGTG CCTGCTACAA GCACTTTTCC 
ACACGAAGGA GTTGGGAGGA GGCAGAAAGC CAGTGCCGAG CGCTAGGGGC TCATCTGACC 
AGCATCTGCA CCCCTGAGGA GCAGGACTTT GTCAACGATC GATACAGGGA GTACCAGTGG 
ATTGGGCTCA ATGACAGGAC CATCGAGGGT GACTTCCTGT GGTCAGATGG TGCCCCTCTG 
CTCTATGAAA ACTGGAACCC TGGGCAGCCT GACAGCTACT TCCTGTCTGG GGAGAACTGT 
GTGGTCATGG TGTGGCATGA CCAGGGACAG TGGAGTGATG TACCCTGCAA CTACCACCTA 
TCCTACACCT GCAAGATGGG GCTTGTGTCA TGTGGACCTC CACCACAGCT GCCCCTGGCT 
CAAATATTTG GTCGCCCTCG GCTGCGCTAC GCGGTGGACA CTGTGCTTCG ATATCGGTGC 
CGAGACGGGC TGGCCCAGCG CAACTTGCCG TTGATCCGCT GCCAGGAGAA TGGGCTTTGG 
GAGGCCCCTC AGATTTCTTG CGTGCCCCGA AGACCTGCCC GTGCTCTCCG CTCAATGACC 
GCCCCAGAAG GACCACGGGG ACAGCTCCCG AGGCAGAGGA AAGCACTGTT GACACCTCCC 
TCCAGTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bcan ( XM_006232596.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232658.1 Rattus norvegicus brevican (Bcan), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa08518
Clone ID Related Accession (Same CDS sequence)	XM_017590643.1 , XM_006232596.3 , NM_001033665.1 , XM_017590645.1 , XM_006232597.3
Accession Version	XM_006232596.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	brevican core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232596.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCCAT TGCTTCTGTC CCTGCTGGCA GCTCTGGTCC TGACCCAAGC CCCTGCAGCC 
CTCGCTGATG ACCTGAAAGA AGACAGCTCA GAGGATCGAG CCTTTCGGGT GCGCATCGGT 
GCCGCGCAGC TGCGGGGTGT GCTGGGCGGT GCCCTGGCCA TCCCATGCCA CGTCCACCAC 
CTGAGGCCGC CGCCCAGCCG CCGGGCCGCG CCGGGCTTTC CCCGAGTCAA ATGGACCTTC 
CTGTCCGGGG ACCGGGAGGT GGAGGTGCTG GTGGCGCGCG GGCTGCGCGT CAAGGTAAAC 
GAAGCCTATC GGTTCCGCGT GGCGCTGCCT GCCTACCCCG CATCGCTCAC AGATGTGTCT 
TTAGTATTGA GCGAACTGCG GCCCAATGAT TCCGGGGTCT ATCGCTGCGA GGTCCAGCAC 
GGTATCGACG ACAGCAGTGA TGCTGTGGAA GTCAAGGTCA AAGGGGTCGT CTTCCTCTAC 
CGAGAGGGCT CTGCCCGCTA TGCTTTCTCC TTCGCTGGAG CCCAGGAAGC CTGTGCTCGC 
ATCGGAGCCC GAATTGCCAC CCCTGAGCAG CTGTATGCTG CCTACCTCGG CGGCTATGAA 
CAGTGTGATG CTGGCTGGCT GTCCGACCAA ACCGTGAGGT ACCCCATCCA GAACCCACGA 
GAAGCCTGTT ATGGAGACAT GGATGGCTAC CCTGGAGTGC GGAATTACGG AGTGGTGGGT 
CCTGATGATC TCTACGATGT CTACTGTTAT GCCGAAGACC TAAATGGAGA ACTGTTCCTA 
GGTGCCCCTC CCGGCAAGCT GACGTGGGAG GAGGCTCGGG ACTACTGTCT GGAACGCGGT 
GCTCAGATCG CTAGCACGGG CCAGCTATAC GCGGCATGGA ATGGCGGCTT GGACAGATGT 
AGCCCTGGCT GGCTGGCTGA TGGCAGTGTG CGGTACCCCA TCATCACGCC CAGCCAACGC 
TGTGGGGGAG GCCTGCCAGG AGTCAAGACC CTCTTCCTCT TTCCCAACCA GACTGGCTTC 
CCCAGCAAGC AGAACCGCTT CAATGTCTAC TGCTTCCGAG ACTCTGCCCA TCCCTCTGCC 
TTCTCTGAGG CCTCCAGCCC AGCCTCTGAT GGACTAGAGG CCATTGTCAC AGTGACAGAG 
AAGCTGGAGG AACTGCAGTT GCCTCAGGAA GCTGTGGAGA GCGAGTCTCG TGGGGCGATC 
TACTCCATCC CCATCACAGA AGATGGGGGA GGAGGAAGCT CTACCCCAGA AGACCCAGCA 
GAGGCCCCCA GGACTCCTCT AGAATCAGAA ACCCAATCCG TTGCACCACC TACCGGGTCC 
TCAGAAGAGG AAGGCGAAGC CCTGGAGGAA GAAGAAAGAT TCAAAGACAC AGAGACTCCG 
AAGGAAGAGA AGGAGCAGGA GAACCTGTGG GTGTGGCCCA CGGAGCTCAG CAGCCCTCTC 
CCTACTGGCT TGGAAACAGA GCACTCACTC TCCCAGGTGT CCCCACCAGC CCAGGCAGTT 
CTACAGCTGG GTGCATCACC TTCTCCCAGG CCTCCAAGGG TCCATGGACC GCCTGCAGAG 
ACTTTGCAAC CCCCAAGGGA GGGAAGCCTC ACATCTACTC CAGATGGGGC AAGAGAAGTA 
GCGGGGGAAA CTGGGAGCCC TGAGCTCTCT GGGGTTCCTC GAGAAAGCGA GGAGGCAGGA 
AGCTCCAGCT TGGAGGATGG CCCTTCCCTC CTTCCAGCGA CATGGGCCCC TGTGGGTACC 
AGGGAGCTGG AGACCCCCTC AGAAGAGAAG TCTGGAAGAA CTGTTCTGAC AGGCACATCA 
GTGCAGGCCC AGCCAGTGCT GCCCACCGAC AGTGCCAGCC GAGGTGGAGT GGCTGTGGCT 
CCCTCATCAG GTGACTGTAT CCCCAGCCCC TGCCACAATG GTGGGACATG CTTGGAGGAG 
AAGGAGGGTT TCCGCTGCCT CTGTTTGCCA GGCTATGGGG GGGACCTGTG CGATGTTGGC 
CTCCACTTCT GCAGCCCGGG CTGGGAGGCC TTCCAGGGTG CCTGCTACAA GCACTTTTCC 
ACACGAAGGA GTTGGGAGGA GGCAGAAAGC CAGTGCCGAG CGCTAGGGGC TCATCTGACC 
AGCATCTGCA CCCCTGAGGA GCAGGACTTT GTCAACGATC GATACAGGGA GTACCAGTGG 
ATTGGGCTCA ATGACAGGAC CATCGAGGGT GACTTCCTGT GGTCAGATGG TGCCCCTCTG 
CTCTATGAAA ACTGGAACCC TGGGCAGCCT GACAGCTACT TCCTGTCTGG GGAGAACTGT 
GTGGTCATGG TGTGGCATGA CCAGGGACAG TGGAGTGATG TACCCTGCAA CTACCACCTA 
TCCTACACCT GCAAGATGGG GCTTGTGTCA TGTGGACCTC CACCACAGCT GCCCCTGGCT 
CAAATATTTG GTCGCCCTCG GCTGCGCTAC GCGGTGGACA CTGTGCTTCG ATATCGGTGC 
CGAGACGGGC TGGCCCAGCG CAACTTGCCG TTGATCCGCT GCCAGGAGAA TGGGCTTTGG 
GAGGCCCCTC AGATTTCTTG CGTGCCCCGA AGACCTGCCC GTGCTCTCCG CTCAATGACC 
GCCCCAGAAG GACCACGGGG ACAGCTCCCG AGGCAGAGGA AAGCACTGTT GACACCTCCC 
TCCAGTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232658.1
CDS	1092..3743
Translation	MIPLLLSLLAALVLTQAPAALADDLKEDSSEDRAFRVRIGAAQLRGVLGGALAIPCHVHHLRPPPSRRAAPGFPRVKWTFLSGDREVEVLVARGLRVKVNEAYRFRVALPAYPASLTDVSLVLSELRPNDSGVYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFAGAQEACARIGARIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQNPREACYGDMDGYPGVRNYGVVGPDDLYDVYCYAEDLNGELFLGAPPGKLTWEEARDYCLERGAQIASTGQLYAAWNGGLDRCSPGWLADGSVRYPIITPSQRCGGGLPGVKTLFLFPNQTGFPSKQNRFNVYCFRDSAHPSAFSEASSPASDGLEAIVTVTEKLEELQLPQEAVESESRGAIYSIPITEDGGGGSSTPEDPAEAPRTPLESETQSVAPPTGSSEEEGEALEEEERFKDTETPKEEKEQENLWVWPTELSSPLPTGLETEHSLSQVSPPAQAVLQLGASPSPRPPRVHGPPAETLQPPREGSLTSTPDGAREVAGETGSPELSGVPRESEEAGSSSLEDGPSLLPATWAPVGTRELETPSEEKSGRTVLTGTSVQAQPVLPTDSASRGGVAVAPSSGDCIPSPCHNGGTCLEEKEGFRCLCLPGYGGDLCDVGLHFCSPGWEAFQGACYKHFSTRRSWEEAESQCRALGAHLTSICTPEEQDFVNDRYREYQWIGLNDRTIEGDFLWSDGAPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQWSDVPCNYHLSYTCKMGLVSCGPPPQLPLAQIFGRPRLRYAVDTVLRYRCRDGLAQRNLPLIRCQENGLWEAPQISCVPRRPARALRSMTAPEGPRGQLPRQRKALLTPPSSL

Target ORF information:

RefSeq Version	XM_006232596.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus brevican (Bcan), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232596.3

ORF Insert Sequence:

ATGATCCCAT TGCTTCTGTC CCTGCTGGCA GCTCTGGTCC TGACCCAAGC CCCTGCAGCC 
CTCGCTGATG ACCTGAAAGA AGACAGCTCA GAGGATCGAG CCTTTCGGGT GCGCATCGGT 
GCCGCGCAGC TGCGGGGTGT GCTGGGCGGT GCCCTGGCCA TCCCATGCCA CGTCCACCAC 
CTGAGGCCGC CGCCCAGCCG CCGGGCCGCG CCGGGCTTTC CCCGAGTCAA ATGGACCTTC 
CTGTCCGGGG ACCGGGAGGT GGAGGTGCTG GTGGCGCGCG GGCTGCGCGT CAAGGTAAAC 
GAAGCCTATC GGTTCCGCGT GGCGCTGCCT GCCTACCCCG CATCGCTCAC AGATGTGTCT 
TTAGTATTGA GCGAACTGCG GCCCAATGAT TCCGGGGTCT ATCGCTGCGA GGTCCAGCAC 
GGTATCGACG ACAGCAGTGA TGCTGTGGAA GTCAAGGTCA AAGGGGTCGT CTTCCTCTAC 
CGAGAGGGCT CTGCCCGCTA TGCTTTCTCC TTCGCTGGAG CCCAGGAAGC CTGTGCTCGC 
ATCGGAGCCC GAATTGCCAC CCCTGAGCAG CTGTATGCTG CCTACCTCGG CGGCTATGAA 
CAGTGTGATG CTGGCTGGCT GTCCGACCAA ACCGTGAGGT ACCCCATCCA GAACCCACGA 
GAAGCCTGTT ATGGAGACAT GGATGGCTAC CCTGGAGTGC GGAATTACGG AGTGGTGGGT 
CCTGATGATC TCTACGATGT CTACTGTTAT GCCGAAGACC TAAATGGAGA ACTGTTCCTA 
GGTGCCCCTC CCGGCAAGCT GACGTGGGAG GAGGCTCGGG ACTACTGTCT GGAACGCGGT 
GCTCAGATCG CTAGCACGGG CCAGCTATAC GCGGCATGGA ATGGCGGCTT GGACAGATGT 
AGCCCTGGCT GGCTGGCTGA TGGCAGTGTG CGGTACCCCA TCATCACGCC CAGCCAACGC 
TGTGGGGGAG GCCTGCCAGG AGTCAAGACC CTCTTCCTCT TTCCCAACCA GACTGGCTTC 
CCCAGCAAGC AGAACCGCTT CAATGTCTAC TGCTTCCGAG ACTCTGCCCA TCCCTCTGCC 
TTCTCTGAGG CCTCCAGCCC AGCCTCTGAT GGACTAGAGG CCATTGTCAC AGTGACAGAG 
AAGCTGGAGG AACTGCAGTT GCCTCAGGAA GCTGTGGAGA GCGAGTCTCG TGGGGCGATC 
TACTCCATCC CCATCACAGA AGATGGGGGA GGAGGAAGCT CTACCCCAGA AGACCCAGCA 
GAGGCCCCCA GGACTCCTCT AGAATCAGAA ACCCAATCCG TTGCACCACC TACCGGGTCC 
TCAGAAGAGG AAGGCGAAGC CCTGGAGGAA GAAGAAAGAT TCAAAGACAC AGAGACTCCG 
AAGGAAGAGA AGGAGCAGGA GAACCTGTGG GTGTGGCCCA CGGAGCTCAG CAGCCCTCTC 
CCTACTGGCT TGGAAACAGA GCACTCACTC TCCCAGGTGT CCCCACCAGC CCAGGCAGTT 
CTACAGCTGG GTGCATCACC TTCTCCCAGG CCTCCAAGGG TCCATGGACC GCCTGCAGAG 
ACTTTGCAAC CCCCAAGGGA GGGAAGCCTC ACATCTACTC CAGATGGGGC AAGAGAAGTA 
GCGGGGGAAA CTGGGAGCCC TGAGCTCTCT GGGGTTCCTC GAGAAAGCGA GGAGGCAGGA 
AGCTCCAGCT TGGAGGATGG CCCTTCCCTC CTTCCAGCGA CATGGGCCCC TGTGGGTACC 
AGGGAGCTGG AGACCCCCTC AGAAGAGAAG TCTGGAAGAA CTGTTCTGAC AGGCACATCA 
GTGCAGGCCC AGCCAGTGCT GCCCACCGAC AGTGCCAGCC GAGGTGGAGT GGCTGTGGCT 
CCCTCATCAG GTGACTGTAT CCCCAGCCCC TGCCACAATG GTGGGACATG CTTGGAGGAG 
AAGGAGGGTT TCCGCTGCCT CTGTTTGCCA GGCTATGGGG GGGACCTGTG CGATGTTGGC 
CTCCACTTCT GCAGCCCGGG CTGGGAGGCC TTCCAGGGTG CCTGCTACAA GCACTTTTCC 
ACACGAAGGA GTTGGGAGGA GGCAGAAAGC CAGTGCCGAG CGCTAGGGGC TCATCTGACC 
AGCATCTGCA CCCCTGAGGA GCAGGACTTT GTCAACGATC GATACAGGGA GTACCAGTGG 
ATTGGGCTCA ATGACAGGAC CATCGAGGGT GACTTCCTGT GGTCAGATGG TGCCCCTCTG 
CTCTATGAAA ACTGGAACCC TGGGCAGCCT GACAGCTACT TCCTGTCTGG GGAGAACTGT 
GTGGTCATGG TGTGGCATGA CCAGGGACAG TGGAGTGATG TACCCTGCAA CTACCACCTA 
TCCTACACCT GCAAGATGGG GCTTGTGTCA TGTGGACCTC CACCACAGCT GCCCCTGGCT 
CAAATATTTG GTCGCCCTCG GCTGCGCTAC GCGGTGGACA CTGTGCTTCG ATATCGGTGC 
CGAGACGGGC TGGCCCAGCG CAACTTGCCG TTGATCCGCT GCCAGGAGAA TGGGCTTTGG 
GAGGCCCCTC AGATTTCTTG CGTGCCCCGA AGACCTGCCC GTGCTCTCCG CTCAATGACC 
GCCCCAGAAG GACCACGGGG ACAGCTCCCG AGGCAGAGGA AAGCACTGTT GACACCTCCC 
TCCAGTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bcan ( XM_017590645.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446134.1 Rattus norvegicus brevican (Bcan), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa08518
Clone ID Related Accession (Same CDS sequence)	XM_017590643.1 , XM_006232596.3 , NM_001033665.1 , XM_017590645.1 , XM_006232597.3
Accession Version	XM_017590645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	brevican core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCCAT TGCTTCTGTC CCTGCTGGCA GCTCTGGTCC TGACCCAAGC CCCTGCAGCC 
CTCGCTGATG ACCTGAAAGA AGACAGCTCA GAGGATCGAG CCTTTCGGGT GCGCATCGGT 
GCCGCGCAGC TGCGGGGTGT GCTGGGCGGT GCCCTGGCCA TCCCATGCCA CGTCCACCAC 
CTGAGGCCGC CGCCCAGCCG CCGGGCCGCG CCGGGCTTTC CCCGAGTCAA ATGGACCTTC 
CTGTCCGGGG ACCGGGAGGT GGAGGTGCTG GTGGCGCGCG GGCTGCGCGT CAAGGTAAAC 
GAAGCCTATC GGTTCCGCGT GGCGCTGCCT GCCTACCCCG CATCGCTCAC AGATGTGTCT 
TTAGTATTGA GCGAACTGCG GCCCAATGAT TCCGGGGTCT ATCGCTGCGA GGTCCAGCAC 
GGTATCGACG ACAGCAGTGA TGCTGTGGAA GTCAAGGTCA AAGGGGTCGT CTTCCTCTAC 
CGAGAGGGCT CTGCCCGCTA TGCTTTCTCC TTCGCTGGAG CCCAGGAAGC CTGTGCTCGC 
ATCGGAGCCC GAATTGCCAC CCCTGAGCAG CTGTATGCTG CCTACCTCGG CGGCTATGAA 
CAGTGTGATG CTGGCTGGCT GTCCGACCAA ACCGTGAGGT ACCCCATCCA GAACCCACGA 
GAAGCCTGTT ATGGAGACAT GGATGGCTAC CCTGGAGTGC GGAATTACGG AGTGGTGGGT 
CCTGATGATC TCTACGATGT CTACTGTTAT GCCGAAGACC TAAATGGAGA ACTGTTCCTA 
GGTGCCCCTC CCGGCAAGCT GACGTGGGAG GAGGCTCGGG ACTACTGTCT GGAACGCGGT 
GCTCAGATCG CTAGCACGGG CCAGCTATAC GCGGCATGGA ATGGCGGCTT GGACAGATGT 
AGCCCTGGCT GGCTGGCTGA TGGCAGTGTG CGGTACCCCA TCATCACGCC CAGCCAACGC 
TGTGGGGGAG GCCTGCCAGG AGTCAAGACC CTCTTCCTCT TTCCCAACCA GACTGGCTTC 
CCCAGCAAGC AGAACCGCTT CAATGTCTAC TGCTTCCGAG ACTCTGCCCA TCCCTCTGCC 
TTCTCTGAGG CCTCCAGCCC AGCCTCTGAT GGACTAGAGG CCATTGTCAC AGTGACAGAG 
AAGCTGGAGG AACTGCAGTT GCCTCAGGAA GCTGTGGAGA GCGAGTCTCG TGGGGCGATC 
TACTCCATCC CCATCACAGA AGATGGGGGA GGAGGAAGCT CTACCCCAGA AGACCCAGCA 
GAGGCCCCCA GGACTCCTCT AGAATCAGAA ACCCAATCCG TTGCACCACC TACCGGGTCC 
TCAGAAGAGG AAGGCGAAGC CCTGGAGGAA GAAGAAAGAT TCAAAGACAC AGAGACTCCG 
AAGGAAGAGA AGGAGCAGGA GAACCTGTGG GTGTGGCCCA CGGAGCTCAG CAGCCCTCTC 
CCTACTGGCT TGGAAACAGA GCACTCACTC TCCCAGGTGT CCCCACCAGC CCAGGCAGTT 
CTACAGCTGG GTGCATCACC TTCTCCCAGG CCTCCAAGGG TCCATGGACC GCCTGCAGAG 
ACTTTGCAAC CCCCAAGGGA GGGAAGCCTC ACATCTACTC CAGATGGGGC AAGAGAAGTA 
GCGGGGGAAA CTGGGAGCCC TGAGCTCTCT GGGGTTCCTC GAGAAAGCGA GGAGGCAGGA 
AGCTCCAGCT TGGAGGATGG CCCTTCCCTC CTTCCAGCGA CATGGGCCCC TGTGGGTACC 
AGGGAGCTGG AGACCCCCTC AGAAGAGAAG TCTGGAAGAA CTGTTCTGAC AGGCACATCA 
GTGCAGGCCC AGCCAGTGCT GCCCACCGAC AGTGCCAGCC GAGGTGGAGT GGCTGTGGCT 
CCCTCATCAG GTGACTGTAT CCCCAGCCCC TGCCACAATG GTGGGACATG CTTGGAGGAG 
AAGGAGGGTT TCCGCTGCCT CTGTTTGCCA GGCTATGGGG GGGACCTGTG CGATGTTGGC 
CTCCACTTCT GCAGCCCGGG CTGGGAGGCC TTCCAGGGTG CCTGCTACAA GCACTTTTCC 
ACACGAAGGA GTTGGGAGGA GGCAGAAAGC CAGTGCCGAG CGCTAGGGGC TCATCTGACC 
AGCATCTGCA CCCCTGAGGA GCAGGACTTT GTCAACGATC GATACAGGGA GTACCAGTGG 
ATTGGGCTCA ATGACAGGAC CATCGAGGGT GACTTCCTGT GGTCAGATGG TGCCCCTCTG 
CTCTATGAAA ACTGGAACCC TGGGCAGCCT GACAGCTACT TCCTGTCTGG GGAGAACTGT 
GTGGTCATGG TGTGGCATGA CCAGGGACAG TGGAGTGATG TACCCTGCAA CTACCACCTA 
TCCTACACCT GCAAGATGGG GCTTGTGTCA TGTGGACCTC CACCACAGCT GCCCCTGGCT 
CAAATATTTG GTCGCCCTCG GCTGCGCTAC GCGGTGGACA CTGTGCTTCG ATATCGGTGC 
CGAGACGGGC TGGCCCAGCG CAACTTGCCG TTGATCCGCT GCCAGGAGAA TGGGCTTTGG 
GAGGCCCCTC AGATTTCTTG CGTGCCCCGA AGACCTGCCC GTGCTCTCCG CTCAATGACC 
GCCCCAGAAG GACCACGGGG ACAGCTCCCG AGGCAGAGGA AAGCACTGTT GACACCTCCC 
TCCAGTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446134.1
CDS	802..3453
Translation	MIPLLLSLLAALVLTQAPAALADDLKEDSSEDRAFRVRIGAAQLRGVLGGALAIPCHVHHLRPPPSRRAAPGFPRVKWTFLSGDREVEVLVARGLRVKVNEAYRFRVALPAYPASLTDVSLVLSELRPNDSGVYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFAGAQEACARIGARIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQNPREACYGDMDGYPGVRNYGVVGPDDLYDVYCYAEDLNGELFLGAPPGKLTWEEARDYCLERGAQIASTGQLYAAWNGGLDRCSPGWLADGSVRYPIITPSQRCGGGLPGVKTLFLFPNQTGFPSKQNRFNVYCFRDSAHPSAFSEASSPASDGLEAIVTVTEKLEELQLPQEAVESESRGAIYSIPITEDGGGGSSTPEDPAEAPRTPLESETQSVAPPTGSSEEEGEALEEEERFKDTETPKEEKEQENLWVWPTELSSPLPTGLETEHSLSQVSPPAQAVLQLGASPSPRPPRVHGPPAETLQPPREGSLTSTPDGAREVAGETGSPELSGVPRESEEAGSSSLEDGPSLLPATWAPVGTRELETPSEEKSGRTVLTGTSVQAQPVLPTDSASRGGVAVAPSSGDCIPSPCHNGGTCLEEKEGFRCLCLPGYGGDLCDVGLHFCSPGWEAFQGACYKHFSTRRSWEEAESQCRALGAHLTSICTPEEQDFVNDRYREYQWIGLNDRTIEGDFLWSDGAPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQWSDVPCNYHLSYTCKMGLVSCGPPPQLPLAQIFGRPRLRYAVDTVLRYRCRDGLAQRNLPLIRCQENGLWEAPQISCVPRRPARALRSMTAPEGPRGQLPRQRKALLTPPSSL

Target ORF information:

RefSeq Version	XM_017590645.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus brevican (Bcan), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590645.1

ORF Insert Sequence:

ATGATCCCAT TGCTTCTGTC CCTGCTGGCA GCTCTGGTCC TGACCCAAGC CCCTGCAGCC 
CTCGCTGATG ACCTGAAAGA AGACAGCTCA GAGGATCGAG CCTTTCGGGT GCGCATCGGT 
GCCGCGCAGC TGCGGGGTGT GCTGGGCGGT GCCCTGGCCA TCCCATGCCA CGTCCACCAC 
CTGAGGCCGC CGCCCAGCCG CCGGGCCGCG CCGGGCTTTC CCCGAGTCAA ATGGACCTTC 
CTGTCCGGGG ACCGGGAGGT GGAGGTGCTG GTGGCGCGCG GGCTGCGCGT CAAGGTAAAC 
GAAGCCTATC GGTTCCGCGT GGCGCTGCCT GCCTACCCCG CATCGCTCAC AGATGTGTCT 
TTAGTATTGA GCGAACTGCG GCCCAATGAT TCCGGGGTCT ATCGCTGCGA GGTCCAGCAC 
GGTATCGACG ACAGCAGTGA TGCTGTGGAA GTCAAGGTCA AAGGGGTCGT CTTCCTCTAC 
CGAGAGGGCT CTGCCCGCTA TGCTTTCTCC TTCGCTGGAG CCCAGGAAGC CTGTGCTCGC 
ATCGGAGCCC GAATTGCCAC CCCTGAGCAG CTGTATGCTG CCTACCTCGG CGGCTATGAA 
CAGTGTGATG CTGGCTGGCT GTCCGACCAA ACCGTGAGGT ACCCCATCCA GAACCCACGA 
GAAGCCTGTT ATGGAGACAT GGATGGCTAC CCTGGAGTGC GGAATTACGG AGTGGTGGGT 
CCTGATGATC TCTACGATGT CTACTGTTAT GCCGAAGACC TAAATGGAGA ACTGTTCCTA 
GGTGCCCCTC CCGGCAAGCT GACGTGGGAG GAGGCTCGGG ACTACTGTCT GGAACGCGGT 
GCTCAGATCG CTAGCACGGG CCAGCTATAC GCGGCATGGA ATGGCGGCTT GGACAGATGT 
AGCCCTGGCT GGCTGGCTGA TGGCAGTGTG CGGTACCCCA TCATCACGCC CAGCCAACGC 
TGTGGGGGAG GCCTGCCAGG AGTCAAGACC CTCTTCCTCT TTCCCAACCA GACTGGCTTC 
CCCAGCAAGC AGAACCGCTT CAATGTCTAC TGCTTCCGAG ACTCTGCCCA TCCCTCTGCC 
TTCTCTGAGG CCTCCAGCCC AGCCTCTGAT GGACTAGAGG CCATTGTCAC AGTGACAGAG 
AAGCTGGAGG AACTGCAGTT GCCTCAGGAA GCTGTGGAGA GCGAGTCTCG TGGGGCGATC 
TACTCCATCC CCATCACAGA AGATGGGGGA GGAGGAAGCT CTACCCCAGA AGACCCAGCA 
GAGGCCCCCA GGACTCCTCT AGAATCAGAA ACCCAATCCG TTGCACCACC TACCGGGTCC 
TCAGAAGAGG AAGGCGAAGC CCTGGAGGAA GAAGAAAGAT TCAAAGACAC AGAGACTCCG 
AAGGAAGAGA AGGAGCAGGA GAACCTGTGG GTGTGGCCCA CGGAGCTCAG CAGCCCTCTC 
CCTACTGGCT TGGAAACAGA GCACTCACTC TCCCAGGTGT CCCCACCAGC CCAGGCAGTT 
CTACAGCTGG GTGCATCACC TTCTCCCAGG CCTCCAAGGG TCCATGGACC GCCTGCAGAG 
ACTTTGCAAC CCCCAAGGGA GGGAAGCCTC ACATCTACTC CAGATGGGGC AAGAGAAGTA 
GCGGGGGAAA CTGGGAGCCC TGAGCTCTCT GGGGTTCCTC GAGAAAGCGA GGAGGCAGGA 
AGCTCCAGCT TGGAGGATGG CCCTTCCCTC CTTCCAGCGA CATGGGCCCC TGTGGGTACC 
AGGGAGCTGG AGACCCCCTC AGAAGAGAAG TCTGGAAGAA CTGTTCTGAC AGGCACATCA 
GTGCAGGCCC AGCCAGTGCT GCCCACCGAC AGTGCCAGCC GAGGTGGAGT GGCTGTGGCT 
CCCTCATCAG GTGACTGTAT CCCCAGCCCC TGCCACAATG GTGGGACATG CTTGGAGGAG 
AAGGAGGGTT TCCGCTGCCT CTGTTTGCCA GGCTATGGGG GGGACCTGTG CGATGTTGGC 
CTCCACTTCT GCAGCCCGGG CTGGGAGGCC TTCCAGGGTG CCTGCTACAA GCACTTTTCC 
ACACGAAGGA GTTGGGAGGA GGCAGAAAGC CAGTGCCGAG CGCTAGGGGC TCATCTGACC 
AGCATCTGCA CCCCTGAGGA GCAGGACTTT GTCAACGATC GATACAGGGA GTACCAGTGG 
ATTGGGCTCA ATGACAGGAC CATCGAGGGT GACTTCCTGT GGTCAGATGG TGCCCCTCTG 
CTCTATGAAA ACTGGAACCC TGGGCAGCCT GACAGCTACT TCCTGTCTGG GGAGAACTGT 
GTGGTCATGG TGTGGCATGA CCAGGGACAG TGGAGTGATG TACCCTGCAA CTACCACCTA 
TCCTACACCT GCAAGATGGG GCTTGTGTCA TGTGGACCTC CACCACAGCT GCCCCTGGCT 
CAAATATTTG GTCGCCCTCG GCTGCGCTAC GCGGTGGACA CTGTGCTTCG ATATCGGTGC 
CGAGACGGGC TGGCCCAGCG CAACTTGCCG TTGATCCGCT GCCAGGAGAA TGGGCTTTGG 
GAGGCCCCTC AGATTTCTTG CGTGCCCCGA AGACCTGCCC GTGCTCTCCG CTCAATGACC 
GCCCCAGAAG GACCACGGGG ACAGCTCCCG AGGCAGAGGA AAGCACTGTT GACACCTCCC 
TCCAGTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bcan ( XM_006232597.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232659.1 Rattus norvegicus brevican (Bcan), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa08518
Clone ID Related Accession (Same CDS sequence)	XM_017590643.1 , XM_006232596.3 , NM_001033665.1 , XM_017590645.1 , XM_006232597.3
Accession Version	XM_006232597.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	brevican core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232597.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCCAT TGCTTCTGTC CCTGCTGGCA GCTCTGGTCC TGACCCAAGC CCCTGCAGCC 
CTCGCTGATG ACCTGAAAGA AGACAGCTCA GAGGATCGAG CCTTTCGGGT GCGCATCGGT 
GCCGCGCAGC TGCGGGGTGT GCTGGGCGGT GCCCTGGCCA TCCCATGCCA CGTCCACCAC 
CTGAGGCCGC CGCCCAGCCG CCGGGCCGCG CCGGGCTTTC CCCGAGTCAA ATGGACCTTC 
CTGTCCGGGG ACCGGGAGGT GGAGGTGCTG GTGGCGCGCG GGCTGCGCGT CAAGGTAAAC 
GAAGCCTATC GGTTCCGCGT GGCGCTGCCT GCCTACCCCG CATCGCTCAC AGATGTGTCT 
TTAGTATTGA GCGAACTGCG GCCCAATGAT TCCGGGGTCT ATCGCTGCGA GGTCCAGCAC 
GGTATCGACG ACAGCAGTGA TGCTGTGGAA GTCAAGGTCA AAGGGGTCGT CTTCCTCTAC 
CGAGAGGGCT CTGCCCGCTA TGCTTTCTCC TTCGCTGGAG CCCAGGAAGC CTGTGCTCGC 
ATCGGAGCCC GAATTGCCAC CCCTGAGCAG CTGTATGCTG CCTACCTCGG CGGCTATGAA 
CAGTGTGATG CTGGCTGGCT GTCCGACCAA ACCGTGAGGT ACCCCATCCA GAACCCACGA 
GAAGCCTGTT ATGGAGACAT GGATGGCTAC CCTGGAGTGC GGAATTACGG AGTGGTGGGT 
CCTGATGATC TCTACGATGT CTACTGTTAT GCCGAAGACC TAAATGGAGA ACTGTTCCTA 
GGTGCCCCTC CCGGCAAGCT GACGTGGGAG GAGGCTCGGG ACTACTGTCT GGAACGCGGT 
GCTCAGATCG CTAGCACGGG CCAGCTATAC GCGGCATGGA ATGGCGGCTT GGACAGATGT 
AGCCCTGGCT GGCTGGCTGA TGGCAGTGTG CGGTACCCCA TCATCACGCC CAGCCAACGC 
TGTGGGGGAG GCCTGCCAGG AGTCAAGACC CTCTTCCTCT TTCCCAACCA GACTGGCTTC 
CCCAGCAAGC AGAACCGCTT CAATGTCTAC TGCTTCCGAG ACTCTGCCCA TCCCTCTGCC 
TTCTCTGAGG CCTCCAGCCC AGCCTCTGAT GGACTAGAGG CCATTGTCAC AGTGACAGAG 
AAGCTGGAGG AACTGCAGTT GCCTCAGGAA GCTGTGGAGA GCGAGTCTCG TGGGGCGATC 
TACTCCATCC CCATCACAGA AGATGGGGGA GGAGGAAGCT CTACCCCAGA AGACCCAGCA 
GAGGCCCCCA GGACTCCTCT AGAATCAGAA ACCCAATCCG TTGCACCACC TACCGGGTCC 
TCAGAAGAGG AAGGCGAAGC CCTGGAGGAA GAAGAAAGAT TCAAAGACAC AGAGACTCCG 
AAGGAAGAGA AGGAGCAGGA GAACCTGTGG GTGTGGCCCA CGGAGCTCAG CAGCCCTCTC 
CCTACTGGCT TGGAAACAGA GCACTCACTC TCCCAGGTGT CCCCACCAGC CCAGGCAGTT 
CTACAGCTGG GTGCATCACC TTCTCCCAGG CCTCCAAGGG TCCATGGACC GCCTGCAGAG 
ACTTTGCAAC CCCCAAGGGA GGGAAGCCTC ACATCTACTC CAGATGGGGC AAGAGAAGTA 
GCGGGGGAAA CTGGGAGCCC TGAGCTCTCT GGGGTTCCTC GAGAAAGCGA GGAGGCAGGA 
AGCTCCAGCT TGGAGGATGG CCCTTCCCTC CTTCCAGCGA CATGGGCCCC TGTGGGTACC 
AGGGAGCTGG AGACCCCCTC AGAAGAGAAG TCTGGAAGAA CTGTTCTGAC AGGCACATCA 
GTGCAGGCCC AGCCAGTGCT GCCCACCGAC AGTGCCAGCC GAGGTGGAGT GGCTGTGGCT 
CCCTCATCAG GTGACTGTAT CCCCAGCCCC TGCCACAATG GTGGGACATG CTTGGAGGAG 
AAGGAGGGTT TCCGCTGCCT CTGTTTGCCA GGCTATGGGG GGGACCTGTG CGATGTTGGC 
CTCCACTTCT GCAGCCCGGG CTGGGAGGCC TTCCAGGGTG CCTGCTACAA GCACTTTTCC 
ACACGAAGGA GTTGGGAGGA GGCAGAAAGC CAGTGCCGAG CGCTAGGGGC TCATCTGACC 
AGCATCTGCA CCCCTGAGGA GCAGGACTTT GTCAACGATC GATACAGGGA GTACCAGTGG 
ATTGGGCTCA ATGACAGGAC CATCGAGGGT GACTTCCTGT GGTCAGATGG TGCCCCTCTG 
CTCTATGAAA ACTGGAACCC TGGGCAGCCT GACAGCTACT TCCTGTCTGG GGAGAACTGT 
GTGGTCATGG TGTGGCATGA CCAGGGACAG TGGAGTGATG TACCCTGCAA CTACCACCTA 
TCCTACACCT GCAAGATGGG GCTTGTGTCA TGTGGACCTC CACCACAGCT GCCCCTGGCT 
CAAATATTTG GTCGCCCTCG GCTGCGCTAC GCGGTGGACA CTGTGCTTCG ATATCGGTGC 
CGAGACGGGC TGGCCCAGCG CAACTTGCCG TTGATCCGCT GCCAGGAGAA TGGGCTTTGG 
GAGGCCCCTC AGATTTCTTG CGTGCCCCGA AGACCTGCCC GTGCTCTCCG CTCAATGACC 
GCCCCAGAAG GACCACGGGG ACAGCTCCCG AGGCAGAGGA AAGCACTGTT GACACCTCCC 
TCCAGTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232659.1
CDS	787..3438
Translation	MIPLLLSLLAALVLTQAPAALADDLKEDSSEDRAFRVRIGAAQLRGVLGGALAIPCHVHHLRPPPSRRAAPGFPRVKWTFLSGDREVEVLVARGLRVKVNEAYRFRVALPAYPASLTDVSLVLSELRPNDSGVYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFAGAQEACARIGARIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQNPREACYGDMDGYPGVRNYGVVGPDDLYDVYCYAEDLNGELFLGAPPGKLTWEEARDYCLERGAQIASTGQLYAAWNGGLDRCSPGWLADGSVRYPIITPSQRCGGGLPGVKTLFLFPNQTGFPSKQNRFNVYCFRDSAHPSAFSEASSPASDGLEAIVTVTEKLEELQLPQEAVESESRGAIYSIPITEDGGGGSSTPEDPAEAPRTPLESETQSVAPPTGSSEEEGEALEEEERFKDTETPKEEKEQENLWVWPTELSSPLPTGLETEHSLSQVSPPAQAVLQLGASPSPRPPRVHGPPAETLQPPREGSLTSTPDGAREVAGETGSPELSGVPRESEEAGSSSLEDGPSLLPATWAPVGTRELETPSEEKSGRTVLTGTSVQAQPVLPTDSASRGGVAVAPSSGDCIPSPCHNGGTCLEEKEGFRCLCLPGYGGDLCDVGLHFCSPGWEAFQGACYKHFSTRRSWEEAESQCRALGAHLTSICTPEEQDFVNDRYREYQWIGLNDRTIEGDFLWSDGAPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQWSDVPCNYHLSYTCKMGLVSCGPPPQLPLAQIFGRPRLRYAVDTVLRYRCRDGLAQRNLPLIRCQENGLWEAPQISCVPRRPARALRSMTAPEGPRGQLPRQRKALLTPPSSL

Target ORF information:

RefSeq Version	XM_006232597.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus brevican (Bcan), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232597.3

ORF Insert Sequence:

ATGATCCCAT TGCTTCTGTC CCTGCTGGCA GCTCTGGTCC TGACCCAAGC CCCTGCAGCC 
CTCGCTGATG ACCTGAAAGA AGACAGCTCA GAGGATCGAG CCTTTCGGGT GCGCATCGGT 
GCCGCGCAGC TGCGGGGTGT GCTGGGCGGT GCCCTGGCCA TCCCATGCCA CGTCCACCAC 
CTGAGGCCGC CGCCCAGCCG CCGGGCCGCG CCGGGCTTTC CCCGAGTCAA ATGGACCTTC 
CTGTCCGGGG ACCGGGAGGT GGAGGTGCTG GTGGCGCGCG GGCTGCGCGT CAAGGTAAAC 
GAAGCCTATC GGTTCCGCGT GGCGCTGCCT GCCTACCCCG CATCGCTCAC AGATGTGTCT 
TTAGTATTGA GCGAACTGCG GCCCAATGAT TCCGGGGTCT ATCGCTGCGA GGTCCAGCAC 
GGTATCGACG ACAGCAGTGA TGCTGTGGAA GTCAAGGTCA AAGGGGTCGT CTTCCTCTAC 
CGAGAGGGCT CTGCCCGCTA TGCTTTCTCC TTCGCTGGAG CCCAGGAAGC CTGTGCTCGC 
ATCGGAGCCC GAATTGCCAC CCCTGAGCAG CTGTATGCTG CCTACCTCGG CGGCTATGAA 
CAGTGTGATG CTGGCTGGCT GTCCGACCAA ACCGTGAGGT ACCCCATCCA GAACCCACGA 
GAAGCCTGTT ATGGAGACAT GGATGGCTAC CCTGGAGTGC GGAATTACGG AGTGGTGGGT 
CCTGATGATC TCTACGATGT CTACTGTTAT GCCGAAGACC TAAATGGAGA ACTGTTCCTA 
GGTGCCCCTC CCGGCAAGCT GACGTGGGAG GAGGCTCGGG ACTACTGTCT GGAACGCGGT 
GCTCAGATCG CTAGCACGGG CCAGCTATAC GCGGCATGGA ATGGCGGCTT GGACAGATGT 
AGCCCTGGCT GGCTGGCTGA TGGCAGTGTG CGGTACCCCA TCATCACGCC CAGCCAACGC 
TGTGGGGGAG GCCTGCCAGG AGTCAAGACC CTCTTCCTCT TTCCCAACCA GACTGGCTTC 
CCCAGCAAGC AGAACCGCTT CAATGTCTAC TGCTTCCGAG ACTCTGCCCA TCCCTCTGCC 
TTCTCTGAGG CCTCCAGCCC AGCCTCTGAT GGACTAGAGG CCATTGTCAC AGTGACAGAG 
AAGCTGGAGG AACTGCAGTT GCCTCAGGAA GCTGTGGAGA GCGAGTCTCG TGGGGCGATC 
TACTCCATCC CCATCACAGA AGATGGGGGA GGAGGAAGCT CTACCCCAGA AGACCCAGCA 
GAGGCCCCCA GGACTCCTCT AGAATCAGAA ACCCAATCCG TTGCACCACC TACCGGGTCC 
TCAGAAGAGG AAGGCGAAGC CCTGGAGGAA GAAGAAAGAT TCAAAGACAC AGAGACTCCG 
AAGGAAGAGA AGGAGCAGGA GAACCTGTGG GTGTGGCCCA CGGAGCTCAG CAGCCCTCTC 
CCTACTGGCT TGGAAACAGA GCACTCACTC TCCCAGGTGT CCCCACCAGC CCAGGCAGTT 
CTACAGCTGG GTGCATCACC TTCTCCCAGG CCTCCAAGGG TCCATGGACC GCCTGCAGAG 
ACTTTGCAAC CCCCAAGGGA GGGAAGCCTC ACATCTACTC CAGATGGGGC AAGAGAAGTA 
GCGGGGGAAA CTGGGAGCCC TGAGCTCTCT GGGGTTCCTC GAGAAAGCGA GGAGGCAGGA 
AGCTCCAGCT TGGAGGATGG CCCTTCCCTC CTTCCAGCGA CATGGGCCCC TGTGGGTACC 
AGGGAGCTGG AGACCCCCTC AGAAGAGAAG TCTGGAAGAA CTGTTCTGAC AGGCACATCA 
GTGCAGGCCC AGCCAGTGCT GCCCACCGAC AGTGCCAGCC GAGGTGGAGT GGCTGTGGCT 
CCCTCATCAG GTGACTGTAT CCCCAGCCCC TGCCACAATG GTGGGACATG CTTGGAGGAG 
AAGGAGGGTT TCCGCTGCCT CTGTTTGCCA GGCTATGGGG GGGACCTGTG CGATGTTGGC 
CTCCACTTCT GCAGCCCGGG CTGGGAGGCC TTCCAGGGTG CCTGCTACAA GCACTTTTCC 
ACACGAAGGA GTTGGGAGGA GGCAGAAAGC CAGTGCCGAG CGCTAGGGGC TCATCTGACC 
AGCATCTGCA CCCCTGAGGA GCAGGACTTT GTCAACGATC GATACAGGGA GTACCAGTGG 
ATTGGGCTCA ATGACAGGAC CATCGAGGGT GACTTCCTGT GGTCAGATGG TGCCCCTCTG 
CTCTATGAAA ACTGGAACCC TGGGCAGCCT GACAGCTACT TCCTGTCTGG GGAGAACTGT 
GTGGTCATGG TGTGGCATGA CCAGGGACAG TGGAGTGATG TACCCTGCAA CTACCACCTA 
TCCTACACCT GCAAGATGGG GCTTGTGTCA TGTGGACCTC CACCACAGCT GCCCCTGGCT 
CAAATATTTG GTCGCCCTCG GCTGCGCTAC GCGGTGGACA CTGTGCTTCG ATATCGGTGC 
CGAGACGGGC TGGCCCAGCG CAACTTGCCG TTGATCCGCT GCCAGGAGAA TGGGCTTTGG 
GAGGCCCCTC AGATTTCTTG CGTGCCCCGA AGACCTGCCC GTGCTCTCCG CTCAATGACC 
GCCCCAGAAG GACCACGGGG ACAGCTCCCG AGGCAGAGGA AAGCACTGTT GACACCTCCC 
TCCAGTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

M?ller glial microRNAs are required for the maintenance of glial homeostasis and retinal architecture.
Nature communications8(1)1603(2017 Nov)
Wohl SG,Jorstad NL,Levine EM,Reh TA

Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
Neuron95(3)639-655.e10(2017 Aug)
Favuzzi E,Marques-Smith A,Deogracias R,Winterflood CM,S?nchez-Aguilera A,Mantoan L,Maeso P,Fernandes C,Ewers H,Rico B

Brevican knockdown reduces late-stage glioma tumor aggressiveness.
Journal of neuro-oncology120(1)63-72(2014 Oct)
Dwyer CA,Bi WL,Viapiano MS,Matthews RT

The role of brevican in glioma: promoting tumor cell motility in vitro and in vivo.
BMC cancer12607(2012 Dec)
Lu R,Wu C,Guo L,Liu Y,Mo W,Wang H,Ding J,Wong ET,Yu M

Alterations in protein regulators of neurodevelopment in the cerebrospinal fluid of infants with posthemorrhagic hydrocephalus of prematurity.
Molecular & cellular proteomics : MCP11(6)M111.011973(2012 Jun)
Morales DM,Townsend RR,Malone JP,Ewersmann CA,Macy EM,Inder TE,Limbrick DD Jr

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Bcan cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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