Gclc cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gclc
Entrez Gene ID	25283
Full Name	glutamate-cysteine ligase, catalytic subunit
Synonyms	Glclc
General protein information	Preferred Names glutamate-cysteine ligase, catalytic subunit Names glutamate--cysteine ligase catalytic subunit GCS heavy chain Glutamylcysteine gamma synthetase light chain gamma-ECS gamma-glutamylcysteine synthetase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q31
Summary	catalyzes the first step in the synthesis of glutathione [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012815.2	NP_036947.1	glutamate--cysteine ligase catalytic subunit

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gclc	NP_036947.1
Saccharomyces cerevisiae (baker's yeast)	GSH1	NP_012434.1
Kluyveromyces lactis	KLLA0F14058g	XP_455711.1
Schizosaccharomyces pombe (fission yeast)	gcs1	NP_593031.1
Magnaporthe oryzae (rice blast fungus)	MGG_07317	XP_003715549.1
Pan troglodytes (chimpanzee)	GCLC	XP_001155930.1
	GCLC	XP_001108943.1
Danio rerio (zebrafish)	gclc	NP_954971.2
Drosophila melanogaster (fruit fly)	Gclc	NP_525005.2
Caenorhabditis elegans (roundworm)	gcs-1	NP_495927.2
Canis lupus familiaris (dog)	GCLC	XP_852845.1
Bos taurus (cattle)	GCLC	NP_001077143.1
Mus musculus (house mouse)	Gclc	NP_034425.1
Gallus gallus (chicken)	GCLC	XP_419910.3
Eremothecium gossypii	AGOS_AFR086C	NP_985633.1
Neurospora crassa	NCU01157	XP_961523.1
Xenopus tropicalis (tropical clawed frog)	gclc	NP_001072803.1
Homo sapiens (human)	GCLC	NP_001489.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001512	XP_321610.3

Gene Ontology
Bibliography

Related articles in PubMed

Effects of troxerutin on cognitive deficits and glutamate cysteine ligase subunits in the hippocampus of streptozotocin-induced type 1 diabetes mellitus rats.
Zhang S, Li H, Zhang L, Li J, Wang R, Wang M
Brain research1657355-360(2017 02)

Epigenetic modifications of Nrf2-mediated glutamate-cysteine ligase: implications for the development of diabetic retinopathy and the metabolic memory phenomenon associated with its continued progression.
Mishra M, Zhong Q, Kowluru RA
Free radical biology & medicine75129-39(2014 Oct)

Schisandra Chinensis Baillon regulates the gene expression of phase II antioxidant/detoxifying enzymes in hepatic damage induced rats.
Jang HI, Do GM, Lee HM, Ok HM, Shin JH, Kwon O
Nutrition research and practice8(3)272-7(2014 Jun)

Polymorphisms in the glutathione pathway modulate cystic fibrosis severity: a cross-sectional study.
Marson FA, Bertuzzo CS, Ribeiro AF, Ribeiro JD
BMC medical genetics1527(2014 Mar)

Development and systematic oxidative stress of a rat model of chronic bronchitis and emphysema induced by biomass smoke.
Hu G, Zhou Y, Hong W, Tian J, Hu J, Peng G, Cui J, Li B, Ran P
Experimental lung research39(6)229-40(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study determined the effects of troxerutin on cognitive deficits and glutamate cysteine ligase subunits (GCLM and GCLC) in the hippocampus of streptozotocin-induced type 1 diabetes mellitus rat: troxerutin alleviates oxidative stress and promotes learning potential in STZ-induced diabetic rats likely through GCL mechanisms, especially that of GCLC.
Title: Effects of troxerutin on cognitive deficits and glutamate cysteine ligase subunits in the hippocampus of streptozotocin-induced type 1 diabetes mellitus rats.

in diabetic retinopathy, histone methylation at Gclc-ARE4 plays an important role in regulating the Nrf2-Gclc-GSH cascade.
Title: Epigenetic modifications of Nrf2-mediated glutamate-cysteine ligase: implications for the development of diabetic retinopathy and the metabolic memory phenomenon associated with its continued progression.

Increased GCLC protein expression in the lungs were observed in the rats exposed to biomass smoke and cigarette smoke.
Title: Development and systematic oxidative stress of a rat model of chronic bronchitis and emphysema induced by biomass smoke.

sumoylation of Nrf2 and MafG enhances heterodimerization and increases GCLC expression, which keeps HSCs in a quiescent state.
Title: Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis.

After exposure to 95% oxygen accumulation of nuclear ATF4 protein in whole lung extracts coincided with induction of glutamate-cysteine ligase catalytic subunit, an intregrated stress response gene.
Title: Hyperoxia-induced activation of the integrated stress response in the newborn rat lung.

The following Gclc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gclc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01015	NM_012815.2 Latest version!	Rattus norvegicus glutamate-cysteine ligase, catalytic subunit (Gclc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gclc ( NM_012815.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036947.1 Rattus norvegicus glutamate-cysteine ligase, catalytic subunit (Gclc), mRNA.

Price & Availability↑

CloneID	ORa01015
Clone ID Related Accession (Same CDS sequence)	NM_012815.2
Accession Version	NM_012815.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	glutamate--cysteine ligase catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081702.1. On Sep 16, 2004 this sequence version replaced NM_012815.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081702.1, J05181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCTGC TGTCCCAAGG CTCGCCACTG AGCTGGGAAG AGACCCAGCG CCACGCCGAC 
CACGTGCGGA GACACGGCAT CCTCCAGTTC CTGCACATCT ACCACGCAGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGTGACGAG GTGGAGTACA TGTTGGTGTC CTTTGATCAT 
GAAAATAGGA AAGTCCAGTT GTTACTGAAT GGCGGCGATG TTCTTGAAAC TCTGCAAGAG 
AAGGGGGAGA GGACAAACCC CAACCACCCA ACCCTCTGGA GACCAGAGTA TGGGAGTTAC 
ATGATTGAAG GGACACCTGG CCAGCCGTAC GGAGGAACGA TGTCCGAGTT CAACACAGTG 
GAGGACAACA TGAGGAAACG CCGGAAGGAG GCTACTTCTG TATTAGGAGA ACATCAGGCT 
CTTTGCACGA TAACTTCATT TCCCAGGCTA GGCTGCCCTG GATTCACACT GCCAGAGCAC 
AGACCCAACC CAGAGGAAGG AGGTGCATCT AAGTCCCTCT TCTTTCCAGA CGAAGCCATA 
AACAAGCACC CCCGCTTTGG TACTCTAACA AGAAACATCC GGCATCGGAG AGGAGAAAAG 
GTTGTCATCA ATGTGCCAAT ATTCAAGGAC AAGAACACAC CATCTCCGTT TGTAGAAACA 
TTTCCTGAGG ATGAGGAGGC ATCAAAGGCC TCTAAGCCAG ACCACATCTA CATGGATGCC 
ATGGGATTTG GGATGGGCAA CTGCTGTCTT CAGGTGACAT TCCAAGCCTG CAGTATATCT 
GAGGCAAGAT ACCTTTATGA CCAGTTGGCC ACTATCTGCC CAATTGTTAT GGCTTTGAGT 
GCTGCATCGC CATTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
TCTGCATCTG TAGATGATAG AACACGGGAG GAGAGAGGAC TGGAGCCCCT GAAGAACAAT 
CGCTTTAAAA TCAGTAAGTC TCGGTATGAC TCAATAGATA GCTACCTGTC CAAGTGTGGA 
GAGAAGTACA ATGACATCGA CCTGACCATC GACACGGAGA TCTACGAGCA GCTCTTAGAG 
GAAGGCATCG ATCACCTTCT GGCACAGCAC GTTGCTCATC TCTTTATTAG AGACCCACTG 
ACCCTTTTTG AAGAGAAAAT TCATCTGGAT GATGCCAACG AGTCTGACCA TTTTGAGAAT 
ATTCAGTCCA CAAACTGGCA GACAATGAGG TTTAAGCCTC CTCCTCCAAA CTCAGATATT 
GGATGGAGAG TAGAGTTCCG ACCAATGGAG GTACAGTTGA CAGACTTTGA GAACTCTGCC 
TATGTGGTAT TTGTGGTACT GCTGACCAGG GTGATCCTCT CATACAAACT AGACTTCCTC 
ATTCCACTGT CCAAGGTTGA CGAGAACATG AAAGTGGCAC AGGAGCGAGA TGCCGTCTTA 
CAGGGGATGT TTTATTTCAG GAAAGACATT TGCAAAGGTG GCAACGCCGT GGTGGATGGG 
TGTAGCAAGG CCCAGACCAG CTCCGAGCCA TCTGCAGAGG AGTACACGCT CATGAGCATA 
GACACCATCA TCAATGGGAA GGAAGGCGTG TTTCCTGGAC TCATCCCCAT TCTGAACTCC 
TACCTTGAAA ACATGGAAGT CGACGTGGAC ACCCGATGCA GTATTCTGAA CTACCTGAAG 
CTAATTAAGA AGAGAGCATC TGGAGAACTA ATGACTGTTG CCAGGTGGAT GAGAGAGTTT 
ATTGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTAATAA CTGATGAGAT CAACTATAGC 
CTCATTTTGA AATGCAATCA AATTGCAAAT GAATTGTGTG AATGTCCAGA GTTACTTGGA 
TCAGGCTTTA GAAAAGCGAA GTACAGTGGA GGTAAAAGCG ACCCTTCAGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036947.1
CDS	64..1977
Translation	MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENRKVQLLLNGGDVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRKEATSVLGEHQALCTITSFPRLGCPGFTLPEHRPNPEEGGASKSLFFPDEAINKHPRFGTLTRNIRHRRGEKVVINVPIFKDKNTPSPFVETFPEDEEASKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNRFKISKSRYDSIDSYLSKCGEKYNDIDLTIDTEIYEQLLEEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQERDAVLQGMFYFRKDICKGGNAVVDGCSKAQTSSEPSAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEINYSLILKCNQIANELCECPELLGSGFRKAKYSGGKSDPSD

Target ORF information:

RefSeq Version	NM_012815.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate-cysteine ligase, catalytic subunit (Gclc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012815.2

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAAGG CTCGCCACTG AGCTGGGAAG AGACCCAGCG CCACGCCGAC 
CACGTGCGGA GACACGGCAT CCTCCAGTTC CTGCACATCT ACCACGCAGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGTGACGAG GTGGAGTACA TGTTGGTGTC CTTTGATCAT 
GAAAATAGGA AAGTCCAGTT GTTACTGAAT GGCGGCGATG TTCTTGAAAC TCTGCAAGAG 
AAGGGGGAGA GGACAAACCC CAACCACCCA ACCCTCTGGA GACCAGAGTA TGGGAGTTAC 
ATGATTGAAG GGACACCTGG CCAGCCGTAC GGAGGAACGA TGTCCGAGTT CAACACAGTG 
GAGGACAACA TGAGGAAACG CCGGAAGGAG GCTACTTCTG TATTAGGAGA ACATCAGGCT 
CTTTGCACGA TAACTTCATT TCCCAGGCTA GGCTGCCCTG GATTCACACT GCCAGAGCAC 
AGACCCAACC CAGAGGAAGG AGGTGCATCT AAGTCCCTCT TCTTTCCAGA CGAAGCCATA 
AACAAGCACC CCCGCTTTGG TACTCTAACA AGAAACATCC GGCATCGGAG AGGAGAAAAG 
GTTGTCATCA ATGTGCCAAT ATTCAAGGAC AAGAACACAC CATCTCCGTT TGTAGAAACA 
TTTCCTGAGG ATGAGGAGGC ATCAAAGGCC TCTAAGCCAG ACCACATCTA CATGGATGCC 
ATGGGATTTG GGATGGGCAA CTGCTGTCTT CAGGTGACAT TCCAAGCCTG CAGTATATCT 
GAGGCAAGAT ACCTTTATGA CCAGTTGGCC ACTATCTGCC CAATTGTTAT GGCTTTGAGT 
GCTGCATCGC CATTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
TCTGCATCTG TAGATGATAG AACACGGGAG GAGAGAGGAC TGGAGCCCCT GAAGAACAAT 
CGCTTTAAAA TCAGTAAGTC TCGGTATGAC TCAATAGATA GCTACCTGTC CAAGTGTGGA 
GAGAAGTACA ATGACATCGA CCTGACCATC GACACGGAGA TCTACGAGCA GCTCTTAGAG 
GAAGGCATCG ATCACCTTCT GGCACAGCAC GTTGCTCATC TCTTTATTAG AGACCCACTG 
ACCCTTTTTG AAGAGAAAAT TCATCTGGAT GATGCCAACG AGTCTGACCA TTTTGAGAAT 
ATTCAGTCCA CAAACTGGCA GACAATGAGG TTTAAGCCTC CTCCTCCAAA CTCAGATATT 
GGATGGAGAG TAGAGTTCCG ACCAATGGAG GTACAGTTGA CAGACTTTGA GAACTCTGCC 
TATGTGGTAT TTGTGGTACT GCTGACCAGG GTGATCCTCT CATACAAACT AGACTTCCTC 
ATTCCACTGT CCAAGGTTGA CGAGAACATG AAAGTGGCAC AGGAGCGAGA TGCCGTCTTA 
CAGGGGATGT TTTATTTCAG GAAAGACATT TGCAAAGGTG GCAACGCCGT GGTGGATGGG 
TGTAGCAAGG CCCAGACCAG CTCCGAGCCA TCTGCAGAGG AGTACACGCT CATGAGCATA 
GACACCATCA TCAATGGGAA GGAAGGCGTG TTTCCTGGAC TCATCCCCAT TCTGAACTCC 
TACCTTGAAA ACATGGAAGT CGACGTGGAC ACCCGATGCA GTATTCTGAA CTACCTGAAG 
CTAATTAAGA AGAGAGCATC TGGAGAACTA ATGACTGTTG CCAGGTGGAT GAGAGAGTTT 
ATTGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTAATAA CTGATGAGAT CAACTATAGC 
CTCATTTTGA AATGCAATCA AATTGCAAAT GAATTGTGTG AATGTCCAGA GTTACTTGGA 
TCAGGCTTTA GAAAAGCGAA GTACAGTGGA GGTAAAAGCG ACCCTTCAGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of troxerutin on cognitive deficits and glutamate cysteine ligase subunits in the hippocampus of streptozotocin-induced type 1 diabetes mellitus rats.
Brain research1657355-360(2017 02)
Zhang S,Li H,Zhang L,Li J,Wang R,Wang M

Epigenetic modifications of Nrf2-mediated glutamate-cysteine ligase: implications for the development of diabetic retinopathy and the metabolic memory phenomenon associated with its continued progression.
Free radical biology & medicine75129-39(2014 Oct)
Mishra M,Zhong Q,Kowluru RA

Schisandra Chinensis Baillon regulates the gene expression of phase II antioxidant/detoxifying enzymes in hepatic damage induced rats.
Nutrition research and practice8(3)272-7(2014 Jun)
Jang HI,Do GM,Lee HM,Ok HM,Shin JH,Kwon O

Polymorphisms in the glutathione pathway modulate cystic fibrosis severity: a cross-sectional study.
BMC medical genetics1527(2014 Mar)
Marson FA,Bertuzzo CS,Ribeiro AF,Ribeiro JD

Development and systematic oxidative stress of a rat model of chronic bronchitis and emphysema induced by biomass smoke.
Experimental lung research39(6)229-40(2013 Aug)
Hu G,Zhou Y,Hong W,Tian J,Hu J,Peng G,Cui J,Li B,Ran P

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Gclc cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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