Cyp24a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp24a1
Entrez Gene ID	25279
Full Name	cytochrome P450, family 24, subfamily a, polypeptide 1
Synonyms	24-OHase,24R-Ohase,Cyp24,P450-CC24,VITD12
General protein information	Preferred Names cytochrome P450, family 24, subfamily a, polypeptide 1 Names 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial 25-hydroxyvitamin D3 24-hydroxylase 25-hydroxyvitaminD324-hydroxylase Cytochrom P450 (25-hydroxyvitamin D24-hydroxylase) cytochrome P450 24A1 cytochrome P450, subfamily 24 cytochrome P450-CC24 vitamin D(3) 24-hydroxylase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42
Summary	enzyme involved in vitamin D metabolism; linked to albuminuria, hyperlipidemia and atherosclerotic aorta lesions [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_201635.3	NP_963966.1	1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CYP24A1	NP_000773.2
	LOC698937	XP_002798136.1
Bos taurus (cattle)	CYP24A1	NP_001178346.1
Pan troglodytes (chimpanzee)	LOC746729	XP_001154704.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008018	XP_317441.2
Mus musculus (house mouse)	Cyp24a1	NP_034126.1
Gallus gallus (chicken)	CYP24A1	NP_990310.1
Danio rerio (zebrafish)	cyp24a1	NP_001082927.1
Drosophila melanogaster (fruit fly)	Cyp12b2	NP_611370.2
Canis lupus familiaris (dog)	CYP24A1	XP_543059.2
Rattus norvegicus (Norway rat)	Cyp24a1	NP_963966.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012800	XP_307282.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008019	XP_555444.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008022	XP_317439.2
Caenorhabditis elegans (roundworm)	cyp-44A1	NP_495052.1
Xenopus tropicalis (tropical clawed frog)	LOC100496967	XP_002941296.2

Gene Ontology
Bibliography

Related articles in PubMed

Loss of miR-125b contributes to upregulation of CYP24 in uraemic rats.
Wang L, Gao Z, Wang L, Gao Y
Nephrology (Carlton, Vic.)21(12)1063-1068(2016 Dec)

Expression pattern of CYP24 in liver during ageing in long-term diabetes.
Vuica A, Vukojevi? K, Ferhatovi? Hamzi? L, Jeri? M, Puljak L, Grkovi? I, Filipovi? N
Acta histochemica118(5)486-95(2016 Jun)

Metabolism of 20-hydroxyvitamin D3 and 20,23-dihydroxyvitamin D3 by rat and human CYP24A1.
Tieu EW, Li W, Chen J, Kim TK, Ma D, Slominski AT, Tuckey RC
The Journal of steroid biochemistry and molecular biology149153-65(2015 May)

High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96.
Zhou YK, Liang Z, Guo Y, Zhang HT, Wang KH
Molecular and cellular endocrinology40475-81(2015 Mar)

Magnesium deficiency regulates vitamin D metabolizing enzymes and type II sodium-phosphate cotransporter mRNA expression in rats.
Matsuzaki H, Katsumata S, Kajita Y, Miwa M
Magnesium research26(2)83-6(2013 Apr-Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Uraemic rats exhibit significant upregulation in basal CYP24 mRNA and protein expression, coupled with decreased miR-125b expression, which may have an impact on vitamin D status.
Title: Loss of miR-125b contributes to upregulation of CYP24 in uraemic rats.

liver has the capacity for an active vitamin D catabolism in different populations of liver cells, especially in sinusoidal endothelial cells, through an expression of CYP24.
Title: Expression pattern of CYP24 in liver during ageing in long-term diabetes.

Human and rat CYP24A1 metabolize 20-hydroxyvitamin D3 to the same dihydroxyvitamin D3 products.
Title: Metabolism of 20-hydroxyvitamin D3 and 20,23-dihydroxyvitamin D3 by rat and human CYP24A1.

Findings suggest that CYP24A1 protein plays an important role in the relationship between diabetic peripheral neuropathy and calcitriol.
Title: High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96.

Magnesium deficiency induces downregulation of 1alpha(OH)ase and type IIa and IIc Na/Pi cotransporters, and upregulation of 24(OH)ase in the kidney.
Title: Magnesium deficiency regulates vitamin D metabolizing enzymes and type II sodium-phosphate cotransporter mRNA expression in rats.

The following Cyp24a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp24a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12475	NM_201635.3 Latest version!	Rattus norvegicus cytochrome P450, family 24, subfamily a, polypeptide 1 (Cyp24a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp24a1 ( NM_201635.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_963966.1 Rattus norvegicus cytochrome P450, family 24, subfamily a, polypeptide 1 (Cyp24a1), mRNA.

Price & Availability↑

CloneID	ORa12475
Clone ID Related Accession (Same CDS sequence)	NM_201635.3
Accession Version	NM_201635.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC100059.1. On or before Apr 13, 2016 this sequence version replaced XM_006235672.2, NM_201635.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC100059.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN02642749 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAGCTGCC CCATTGACAA ACGGCGCACC CTGATCGCTT TTCTGCGCCG ACTGCGCGAC 
CTGGGACAGC CCCCAAGGTC GGTGACATCC AAGGCGAGCG CTTCCCGCGC TCCAAAAGAG 
GTGCCCCTCT GCCCGCTGAT GACAGACGGT GAGACTCGAA ACGTCACCTC CTTGCCTGGG 
CCCACCAACT GGCCACTGCT AGGGAGTCTA CTGGAGATTT TTTGGAAAGG TGGCCTGAAG 
AAACAGCACG ACACGCTGGC AGAGTACCAC AAGAAGTATG GCCAGATTTT CCGGATGAAG 
CTGGGCTCCT TCGACTCCGT GCATCTGGGC TCGCCGAGCC TGCTGGAAGC TCTGTACCGC 
ACAGAGAGCG CGCATCCCCA GCGACTGGAG ATCAAACCTT GGAAAGCCTA TCGCGACCAC 
AGGAACGAAG CCTACGGGTT GATGATCCTG GAAGGACAGG AGTGGCAAAG GGTCCGCAGC 
GCCTTCCAGA AGAAACTCAT GAAGCCCGTG GAGATCATGA AGCTGGACAA GAAAATCAAT 
GAGGTCTTGG CTGACTTTCT AGAGCGCATG GATGAGCTGT GCGATGAGCG AGGCCGCATC 
CCAGACCTGT ACAGTGAGCT GAACAAGTGG TCCTTTGAAA GCATCTGCCT TGTGTTATAT 
GAGAAGAGAT TTGGGCTCCT TCAAAAGGAG ACAGAAGAAG AAGCGCTGAC CTTCATTACG 
GCCATCAAAA CGATGATGAG CACGTTTGGG AAGATGATGG TGACCCCCGT GGAGTTGCAC 
AAGCGCCTCA ACACCAAAGT GTGGCAGGCA CATACGCTGG CTTGGGACAC CATTTTCAAA 
TCAGTCAAAC CCTGCATCGA CAACCGCCTA CAGAGATATT CCCAGCAGCC CGGGGCAGAT 
TTCCTCTGTG ATATTTATCA GCAAGATCAC CTTTCCAAGA AGGAACTGTA CGCCGCTGTC 
ACGGAGCTGC AGCTCGCTGC AGTGGAGACG ACCGCGAACA GCTTGATGTG GATTCTCTAC 
AATCTATCCC GGAATCCTCA AGCGCAACGG AGACTCCTTC AGGAAGTTCA GAGCGTGCTG 
CCTGACAATC AGACGCCACG GGCGGAAGAC CTGAGGAATA TGCCCTATTT AAAGGCCTGT 
CTGAAAGAAT CCATGAGGCT TACCCCAAGT GTGCCATTTA CAACTCGGAC CCTTGACAAA 
CCAACGGTTC TGGGTGAATA CGCTCTACCC AAAGGAACAG TGTTAACCCT CAATACCCAA 
GTGCTGGGTT CTAGTGAAGA CAATTTTGAA GATTCTCATA AGTTCAGACC CGAACGCTGG 
CTTCAAAAGG AGAAAAAGAT CAACCCCTTC GCTCATCTCC CATTCGGCAT CGGGAAGAGA 
ATGTGCATCG GGCGCCGGCT GGCTGAGTTA CAGCTCCACC TGGCTCTCTG CTGGATAATC 
CAAAAATACG ACATTGTGGC CACAGACAAT GAACCTGTGG AGATGCTGCA CCTTGGCATC 
CTGGTACCCA GCCGGGAGCT GCCAATTGCG TTCCGTCCAC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963966.1
CDS	346..1890
Translation	MSCPIDKRRTLIAFLRRLRDLGQPPRSVTSKASASRAPKEVPLCPLMTDGETRNVTSLPGPTNWPLLGSLLEIFWKGGLKKQHDTLAEYHKKYGQIFRMKLGSFDSVHLGSPSLLEALYRTESAHPQRLEIKPWKAYRDHRNEAYGLMILEGQEWQRVRSAFQKKLMKPVEIMKLDKKINEVLADFLERMDELCDERGRIPDLYSELNKWSFESICLVLYEKRFGLLQKETEEEALTFITAIKTMMSTFGKMMVTPVELHKRLNTKVWQAHTLAWDTIFKSVKPCIDNRLQRYSQQPGADFLCDIYQQDHLSKKELYAAVTELQLAAVETTANSLMWILYNLSRNPQAQRRLLQEVQSVLPDNQTPRAEDLRNMPYLKACLKESMRLTPSVPFTTRTLDKPTVLGEYALPKGTVLTLNTQVLGSSEDNFEDSHKFRPERWLQKEKKINPFAHLPFGIGKRMCIGRRLAELQLHLALCWIIQKYDIVATDNEPVEMLHLGILVPSRELPIAFRPR

Target ORF information:

RefSeq Version	NM_201635.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 24, subfamily a, polypeptide 1 (Cyp24a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201635.3

ORF Insert Sequence:

ATGAGCTGCC CCATTGACAA ACGGCGCACC CTGATCGCTT TTCTGCGCCG ACTGCGCGAC 
CTGGGACAGC CCCCAAGGTC GGTGACATCC AAGGCGAGCG CTTCCCGCGC TCCAAAAGAG 
GTGCCCCTCT GCCCGCTGAT GACAGACGGT GAGACTCGAA ACGTCACCTC CTTGCCTGGG 
CCCACCAACT GGCCACTGCT AGGGAGTCTA CTGGAGATTT TTTGGAAAGG TGGCCTGAAG 
AAACAGCACG ACACGCTGGC AGAGTACCAC AAGAAGTATG GCCAGATTTT CCGGATGAAG 
CTGGGCTCCT TCGACTCCGT GCATCTGGGC TCGCCGAGCC TGCTGGAAGC TCTGTACCGC 
ACAGAGAGCG CGCATCCCCA GCGACTGGAG ATCAAACCTT GGAAAGCCTA TCGCGACCAC 
AGGAACGAAG CCTACGGGTT GATGATCCTG GAAGGACAGG AGTGGCAAAG GGTCCGCAGC 
GCCTTCCAGA AGAAACTCAT GAAGCCCGTG GAGATCATGA AGCTGGACAA GAAAATCAAT 
GAGGTCTTGG CTGACTTTCT AGAGCGCATG GATGAGCTGT GCGATGAGCG AGGCCGCATC 
CCAGACCTGT ACAGTGAGCT GAACAAGTGG TCCTTTGAAA GCATCTGCCT TGTGTTATAT 
GAGAAGAGAT TTGGGCTCCT TCAAAAGGAG ACAGAAGAAG AAGCGCTGAC CTTCATTACG 
GCCATCAAAA CGATGATGAG CACGTTTGGG AAGATGATGG TGACCCCCGT GGAGTTGCAC 
AAGCGCCTCA ACACCAAAGT GTGGCAGGCA CATACGCTGG CTTGGGACAC CATTTTCAAA 
TCAGTCAAAC CCTGCATCGA CAACCGCCTA CAGAGATATT CCCAGCAGCC CGGGGCAGAT 
TTCCTCTGTG ATATTTATCA GCAAGATCAC CTTTCCAAGA AGGAACTGTA CGCCGCTGTC 
ACGGAGCTGC AGCTCGCTGC AGTGGAGACG ACCGCGAACA GCTTGATGTG GATTCTCTAC 
AATCTATCCC GGAATCCTCA AGCGCAACGG AGACTCCTTC AGGAAGTTCA GAGCGTGCTG 
CCTGACAATC AGACGCCACG GGCGGAAGAC CTGAGGAATA TGCCCTATTT AAAGGCCTGT 
CTGAAAGAAT CCATGAGGCT TACCCCAAGT GTGCCATTTA CAACTCGGAC CCTTGACAAA 
CCAACGGTTC TGGGTGAATA CGCTCTACCC AAAGGAACAG TGTTAACCCT CAATACCCAA 
GTGCTGGGTT CTAGTGAAGA CAATTTTGAA GATTCTCATA AGTTCAGACC CGAACGCTGG 
CTTCAAAAGG AGAAAAAGAT CAACCCCTTC GCTCATCTCC CATTCGGCAT CGGGAAGAGA 
ATGTGCATCG GGCGCCGGCT GGCTGAGTTA CAGCTCCACC TGGCTCTCTG CTGGATAATC 
CAAAAATACG ACATTGTGGC CACAGACAAT GAACCTGTGG AGATGCTGCA CCTTGGCATC 
CTGGTACCCA GCCGGGAGCT GCCAATTGCG TTCCGTCCAC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of miR-125b contributes to upregulation of CYP24 in uraemic rats.
Nephrology (Carlton, Vic.)21(12)1063-1068(2016 Dec)
Wang L,Gao Z,Wang L,Gao Y

Expression pattern of CYP24 in liver during ageing in long-term diabetes.
Acta histochemica118(5)486-95(2016 Jun)
Vuica A,Vukojevi? K,Ferhatovi? Hamzi? L,Jeri? M,Puljak L,Grkovi? I,Filipovi? N

Metabolism of 20-hydroxyvitamin D3 and 20,23-dihydroxyvitamin D3 by rat and human CYP24A1.
The Journal of steroid biochemistry and molecular biology149153-65(2015 May)
Tieu EW,Li W,Chen J,Kim TK,Ma D,Slominski AT,Tuckey RC

High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96.
Molecular and cellular endocrinology40475-81(2015 Mar)
Zhou YK,Liang Z,Guo Y,Zhang HT,Wang KH

Magnesium deficiency regulates vitamin D metabolizing enzymes and type II sodium-phosphate cotransporter mRNA expression in rats.
Magnesium research26(2)83-6(2013 Apr-Jun)
Matsuzaki H,Katsumata S,Kajita Y,Miwa M

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Cyp24a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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