Dlg1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dlg1
Entrez Gene ID	25252
Full Name	discs large MAGUK scaffold protein 1
Synonyms	Dlgh1,SAP97
General protein information	Preferred Names discs large MAGUK scaffold protein 1 Names disks large homolog 1 Drosophila discs-large tumor suppressor homologue (synapse associated protein) SAP-97 discs large homolog 1, scribble cell polarity complex component discs, large homolog 1 synapse-associated protein 97
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q22
Summary	putative cell adhesion molecule, member of the membrane-associated guanylate kinase protein family; binds to NMDA receptors to facilitate NMDA receptor-regulated synapse targeting [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012788.1	NP_036920.1	disks large homolog 1

Pathways from BioSystems

Homologies

	DLG1	XP_001098808.1
Danio rerio (zebrafish)	dlg1	NP_955820.1
Drosophila melanogaster (fruit fly)	dlg1	NP_996406.1
Pan troglodytes (chimpanzee)	DLG1	XP_001166204.1
Canis lupus familiaris (dog)	DLG1	XP_545159.2
Gallus gallus (chicken)	DLG1	XP_004943373.1
Xenopus tropicalis (tropical clawed frog)	dlg1	NP_001039116.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000255	XP_003436951.1
Homo sapiens (human)	DLG1	NP_004078.2
Bos taurus (cattle)	DLG1	XP_002684901.2
Mus musculus (house mouse)	Dlg1	NP_031888.2
Rattus norvegicus (Norway rat)	Dlg1	NP_036920.1

Gene Ontology
Bibliography

Related articles in PubMed

Kif13b Regulates PNS and CNS Myelination through the Dlg1 Scaffold.
Noseda R, Guerrero-Valero M, Alberizzi V, Previtali SC, Sherman DL, Palmisano M, Huganir RL, Nave KA, Cuenda A, Feltri ML, Brophy PJ, Bolino A
PLoS biology14(4)e1002440(2016 Apr)

A Critical Role for the GluA1 Accessory Protein, SAP97, in Cocaine Seeking.
White SL, Ortinski PI, Friedman SH, Zhang L, Neve RL, Kalb RG, Schmidt HD, Pierce RC
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology41(3)736-50(2016 Feb)

Structure-function analysis of SAP97, a modular scaffolding protein that drives dendrite growth.
Zhang L, Hsu FC, Mojsilovic-Petrovic J, Jablonski AM, Zhai J, Coulter DA, Kalb RG
Molecular and cellular neurosciences6531-44(2015 Mar)

Corticotropin-Releasing Hormone Receptor Type 1 (CRHR1) Clustering with MAGUKs Is Mediated via Its C-Terminal PDZ Binding Motif.
Bender J, Engeholm M, Ederer MS, Breu J, M?ller TC, Michalakis S, Rasko T, Wanker EE, Biel M, Martinez KL, Wurst W, Deussing JM
PloS one10(9)e0136768(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that the GluA1 subunit accessory protein SAP97 may represent a novel target for pharmacotherapeutic intervention in the treatment of cocaine craving
Title: A Critical Role for the GluA1 Accessory Protein, SAP97, in Cocaine Seeking.

dendrite branching promoting action of full length SAP97 depends on ligand(s) that bind to the PDZ3 domain.
Title: Structure-function analysis of SAP97, a modular scaffolding protein that drives dendrite growth.

studies show that SAP97 interactions with CRFR1 attenuate CRFR1 endocytosis and that SAP97 is involved in coupling G protein-coupled receptors to the activation of the ERK1/2 signaling pathway.
Title: Role of SAP97 protein in the regulation of corticotropin-releasing factor receptor 1 endocytosis and extracellular signal-regulated kinase 1/2 signaling.

Acute BDNF treatment upregulates the interactions between AMPA receptor subunits (GluR1 and GluR2) with their scaffold proteins SAP97 and GRIP1, respectively.
Title: Acute BDNF treatment upregulates GluR1-SAP97 and GluR2-GRIP1 interactions: implications for sustained AMPA receptor expression.

The data establish a prominent role for PDZ2 and I3 domains of SAP97 in organizing the ss1-adrenergic receptosome involved in connecting the ss1-AR to trafficking and signaling networks.
Title: SAP97 controls the trafficking and resensitization of the beta-1-adrenergic receptor through its PDZ2 and I3 domains.

The following Dlg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dlg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13432	NM_012788.1 Latest version!	Rattus norvegicus discs large MAGUK scaffold protein 1 (Dlg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$538.30 $769.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dlg1 ( NM_012788.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036920.1 Rattus norvegicus discs large MAGUK scaffold protein 1 (Dlg1), mRNA.

Price & Availability↑

CloneID	ORa13432
Clone ID Related Accession (Same CDS sequence)	NM_012788.1
Accession Version	NM_012788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	disks large homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U14950.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U14950.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGTCC GGAAGCAAGA TACCCAGAGA GCATTGCATC TGTTGGAAGA ATATCGGTCG 
AAACTAAGCC AAACCGAAGA CAGACAGCTC AGGAGTTCCA TAGAGCGGGT TATTAGCATA 
TTTCAGAGCA ACCTCTTTCA GGCTTTAATA GACATTCAAG AATTTTATGA AGTGACCTTA 
CTTGATAATC CAAAATGTGT GGATCATTCA AAGCAGTGTG AACCGGTCCA ACCCGGGAAT 
CCTTGGGAGA GTGGCAGCCT TTCAAGTGCT GCTGTGACTT CAGAGAGCCT GCCCGGCGGC 
CTTAGCCCTC CAGTAGAGAA ATACCGGTAT CAGGACGAAG AGGTGCTGCC TTCAGAGCGG 
ATTTCCCCGC AAGTCCCAAA TGAGGTGCTG GGTCCGGAGC TGGTTCACGT CTCGGAGAAG 
AGCCTGTCAG AGATTGAGAA TGTCCACGGG TTTGTCTCTC ACTCTCATAT CTCACCCATA 
AAGCCCACAG AAGCTGTTCC TCCCTCCTCT CCCATTGTCC CTGTGACCCC TGCCTTGCCA 
GTCCCTGCTG AGAGTCCTGT CGTCCTGCCC TCCACACCAC AGGCAAATCC TCCTCCTGTC 
CTGGTCAACA CAGACAGCTT GGAGACACCA ACTTATGTTA ATGGTACTGA TGCAGATTAT 
GAATATGAGG AAATCACACT TGAAAGGGGA AATTCAGGCT TGGGTTTCAG CATTGCAGGA 
GGTACAGACA ACCCACACAT TGGAGATGAC TCAAGTATTT TCATCACCAA AATTATCACG 
GGAGGAGCAG CTGCCCAGGA TGGAAGATTG CGGGTAAATG ACTGTATACT GAGAGTAAAT 
GAAGCAGATG TTCGTGATGT AACCCACAGC AAAGCAGTTG AAGCATTGAA AGAAGCTGGA 
TCGATAGTGC GATTATATGT GAAAAGGCGG AAGGCCTTCA GAAAAAATCA TGAAATAAAA 
CTTATTAAAG GCCCTAAAGG TCTTGGGTTC AGCATTGCTG GAGGTGTTGG AAACCAGCAC 
ATTCCTGGGG ATAACAGCAT CTATGTAACC AAAATAATTG AAGGAGGTGC AGCACACAAG 
GACGGCAAAC TTCAGATTGG AGACAAGCTT CTAGCAGTGA ACAGTGTCTG TTTAGAAGAA 
GTTACTCATG AAGAAGCAGT GACTGCCTTA AAGAATACAT CTGATTTTGT TTATTTGAAA 
GCGGCAAAAC CAACAAGTAT GTATATAAAT GATGGCTATG CACCACCCGA CATCACTAAT 
TCTTCTTCTC AGTCTGTTGA CAACCATGTT AGCCCGTCTT CATACTTGGG CCAGACTCCA 
GCGTCACCAG CCAGATACTC ACCCATTTCT AAAGCAGTGC TTGGAGATGA TGAGATCACT 
AGGGAACCTA GAAAAGTTGT TCTTCATCGT GGCTCAACGG GACTTGGTTT CAACATTGTG 
GGAGGCGAAG ATGGAGAAGG GATTTTTATC TCTTTCATCC TTGCTGGGGG ACCTGCTGAC 
CTAAGCGGAG AGCTCAGAAA AGGAGATCGC ATTATATCGG TAAACAGTGT TGACCTCAGA 
GCTGCAAGTC ATGAACAAGC AGCTGCCGCA TTAAAAAACG CAGGCCAAGC TGTCACTATT 
GTCGCACAGT ATCGGCCTGA AGAGTACAGT CGTTTTGAAG CTAAAATTCA CGACTTGCGG 
GAGACGATGA TGAACAGCAG TGTCAGCTCA GGGTCAGGGT CTCTCCGAAC CAGCCAAAAG 
CGGTCCCTCT ATGTCAGAGC CCTTTTTGAT TACGACAAGA CTAAAGACAG TGGCCTTCCC 
AGTCAAGGGT TGAACTTCAA ATTCGGAGAT ATTCTCCATG TTATCAATGC TTCTGATGAC 
GAGTGGTGGC AGGCCAGACA GGTGACCCCA GATGGTGAGA GTGACGAAGT TGGAGTAATT 
CCTAGTAAAC GCAGAGTTGA GAAGAAAGAA CGAGCCCGAT TAAAAACAGT CAAATTCAAT 
TCTAAAACAA GAGGAGATAA AGGGGAGATC CCTGACGACA TGGGATCAAA AGGCCTGAAG 
CACGTAACTT CTAATGCCAG CGATAGCGAA AGTAGTTACC ATGAATATGG CTGCTCAAAA 
GGTGGTCAAG AAGAATATGT TTTATCGTAT GAGCCGGTGA ATCAGCAAGA AGTTAATTAT 
ACCCGACCAG TCATCATATT AGGACCTATG AAAGACAGAG TAAATGATGA CTTAATCTCA 
GAATTTCCTG ACAAATTTGG ATCCTGTGTC CCTCATACAA CTAGACCAAA GCGTGACTAT 
GAGGTGGATG GACGAGATTA CCATTTTGTG ACTTCAAGAG AACAGATGGA GAAAGATATC 
CAGGAGCATA AATTCATTGA AGCTGGCCAG TATAACAACC ATCTATACGG AACGAGTGTC 
CAATCTGTGC GAGCAGTAGC AGAGAAGGGC AAGCATTGTA TCCTTGATGT GTCTGGAAAT 
GCCATCAAGA GGTTGCAGAT TGCACAGCTC TATCCAATAT CTATTTTTAT TAAACCCAAA 
TCCATGGAAA ATATCATGGA AATGAATAAA CGCCTAACAG ACGAACAGGC CAGAAAAACG 
TTTGAGAGAG CAGTGAGACT GGAGCAGGAG TTCACTGAGC ATTTCACAGC TATTGTCCAG 
GGAGACACGC TGGAGGACAT TTACAATCAA GTGAAGCAGA TCATAGAAGA GCAGTCTGGT 
CCTTACATCT GGGTCCCAGC AAAAGAAAAA TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036920.1
CDS	452..3187
Translation	MPVRKQDTQRALHLLEEYRSKLSQTEDRQLRSSIERVISIFQSNLFQALIDIQEFYEVTLLDNPKCVDHSKQCEPVQPGNPWESGSLSSAAVTSESLPGGLSPPVEKYRYQDEEVLPSERISPQVPNEVLGPELVHVSEKSLSEIENVHGFVSHSHISPIKPTEAVPPSSPIVPVTPALPVPAESPVVLPSTPQANPPPVLVNTDSLETPTYVNGTDADYEYEEITLERGNSGLGFSIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEADVRDVTHSKAVEALKEAGSIVRLYVKRRKAFRKNHEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNSVCLEEVTHEEAVTALKNTSDFVYLKAAKPTSMYINDGYAPPDITNSSSQSVDNHVSPSSYLGQTPASPARYSPISKAVLGDDEITREPRKVVLHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAASHEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKIHDLRETMMNSSVSSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFKFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGEIPDDMGSKGLKHVTSNASDSESSYHEYGCSKGGQEEYVLSYEPVNQQEVNYTRPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVRAVAEKGKHCILDVSGNAIKRLQIAQLYPISIFIKPKSMENIMEMNKRLTDEQARKTFERAVRLEQEFTEHFTAIVQGDTLEDIYNQVKQIIEEQSGPYIWVPAKEKL

Target ORF information:

RefSeq Version	NM_012788.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus discs large MAGUK scaffold protein 1 (Dlg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012788.1

ORF Insert Sequence:

ATGCCGGTCC GGAAGCAAGA TACCCAGAGA GCATTGCATC TGTTGGAAGA ATATCGGTCG 
AAACTAAGCC AAACCGAAGA CAGACAGCTC AGGAGTTCCA TAGAGCGGGT TATTAGCATA 
TTTCAGAGCA ACCTCTTTCA GGCTTTAATA GACATTCAAG AATTTTATGA AGTGACCTTA 
CTTGATAATC CAAAATGTGT GGATCATTCA AAGCAGTGTG AACCGGTCCA ACCCGGGAAT 
CCTTGGGAGA GTGGCAGCCT TTCAAGTGCT GCTGTGACTT CAGAGAGCCT GCCCGGCGGC 
CTTAGCCCTC CAGTAGAGAA ATACCGGTAT CAGGACGAAG AGGTGCTGCC TTCAGAGCGG 
ATTTCCCCGC AAGTCCCAAA TGAGGTGCTG GGTCCGGAGC TGGTTCACGT CTCGGAGAAG 
AGCCTGTCAG AGATTGAGAA TGTCCACGGG TTTGTCTCTC ACTCTCATAT CTCACCCATA 
AAGCCCACAG AAGCTGTTCC TCCCTCCTCT CCCATTGTCC CTGTGACCCC TGCCTTGCCA 
GTCCCTGCTG AGAGTCCTGT CGTCCTGCCC TCCACACCAC AGGCAAATCC TCCTCCTGTC 
CTGGTCAACA CAGACAGCTT GGAGACACCA ACTTATGTTA ATGGTACTGA TGCAGATTAT 
GAATATGAGG AAATCACACT TGAAAGGGGA AATTCAGGCT TGGGTTTCAG CATTGCAGGA 
GGTACAGACA ACCCACACAT TGGAGATGAC TCAAGTATTT TCATCACCAA AATTATCACG 
GGAGGAGCAG CTGCCCAGGA TGGAAGATTG CGGGTAAATG ACTGTATACT GAGAGTAAAT 
GAAGCAGATG TTCGTGATGT AACCCACAGC AAAGCAGTTG AAGCATTGAA AGAAGCTGGA 
TCGATAGTGC GATTATATGT GAAAAGGCGG AAGGCCTTCA GAAAAAATCA TGAAATAAAA 
CTTATTAAAG GCCCTAAAGG TCTTGGGTTC AGCATTGCTG GAGGTGTTGG AAACCAGCAC 
ATTCCTGGGG ATAACAGCAT CTATGTAACC AAAATAATTG AAGGAGGTGC AGCACACAAG 
GACGGCAAAC TTCAGATTGG AGACAAGCTT CTAGCAGTGA ACAGTGTCTG TTTAGAAGAA 
GTTACTCATG AAGAAGCAGT GACTGCCTTA AAGAATACAT CTGATTTTGT TTATTTGAAA 
GCGGCAAAAC CAACAAGTAT GTATATAAAT GATGGCTATG CACCACCCGA CATCACTAAT 
TCTTCTTCTC AGTCTGTTGA CAACCATGTT AGCCCGTCTT CATACTTGGG CCAGACTCCA 
GCGTCACCAG CCAGATACTC ACCCATTTCT AAAGCAGTGC TTGGAGATGA TGAGATCACT 
AGGGAACCTA GAAAAGTTGT TCTTCATCGT GGCTCAACGG GACTTGGTTT CAACATTGTG 
GGAGGCGAAG ATGGAGAAGG GATTTTTATC TCTTTCATCC TTGCTGGGGG ACCTGCTGAC 
CTAAGCGGAG AGCTCAGAAA AGGAGATCGC ATTATATCGG TAAACAGTGT TGACCTCAGA 
GCTGCAAGTC ATGAACAAGC AGCTGCCGCA TTAAAAAACG CAGGCCAAGC TGTCACTATT 
GTCGCACAGT ATCGGCCTGA AGAGTACAGT CGTTTTGAAG CTAAAATTCA CGACTTGCGG 
GAGACGATGA TGAACAGCAG TGTCAGCTCA GGGTCAGGGT CTCTCCGAAC CAGCCAAAAG 
CGGTCCCTCT ATGTCAGAGC CCTTTTTGAT TACGACAAGA CTAAAGACAG TGGCCTTCCC 
AGTCAAGGGT TGAACTTCAA ATTCGGAGAT ATTCTCCATG TTATCAATGC TTCTGATGAC 
GAGTGGTGGC AGGCCAGACA GGTGACCCCA GATGGTGAGA GTGACGAAGT TGGAGTAATT 
CCTAGTAAAC GCAGAGTTGA GAAGAAAGAA CGAGCCCGAT TAAAAACAGT CAAATTCAAT 
TCTAAAACAA GAGGAGATAA AGGGGAGATC CCTGACGACA TGGGATCAAA AGGCCTGAAG 
CACGTAACTT CTAATGCCAG CGATAGCGAA AGTAGTTACC ATGAATATGG CTGCTCAAAA 
GGTGGTCAAG AAGAATATGT TTTATCGTAT GAGCCGGTGA ATCAGCAAGA AGTTAATTAT 
ACCCGACCAG TCATCATATT AGGACCTATG AAAGACAGAG TAAATGATGA CTTAATCTCA 
GAATTTCCTG ACAAATTTGG ATCCTGTGTC CCTCATACAA CTAGACCAAA GCGTGACTAT 
GAGGTGGATG GACGAGATTA CCATTTTGTG ACTTCAAGAG AACAGATGGA GAAAGATATC 
CAGGAGCATA AATTCATTGA AGCTGGCCAG TATAACAACC ATCTATACGG AACGAGTGTC 
CAATCTGTGC GAGCAGTAGC AGAGAAGGGC AAGCATTGTA TCCTTGATGT GTCTGGAAAT 
GCCATCAAGA GGTTGCAGAT TGCACAGCTC TATCCAATAT CTATTTTTAT TAAACCCAAA 
TCCATGGAAA ATATCATGGA AATGAATAAA CGCCTAACAG ACGAACAGGC CAGAAAAACG 
TTTGAGAGAG CAGTGAGACT GGAGCAGGAG TTCACTGAGC ATTTCACAGC TATTGTCCAG 
GGAGACACGC TGGAGGACAT TTACAATCAA GTGAAGCAGA TCATAGAAGA GCAGTCTGGT 
CCTTACATCT GGGTCCCAGC AAAAGAAAAA TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Kif13b Regulates PNS and CNS Myelination through the Dlg1 Scaffold.
PLoS biology14(4)e1002440(2016 Apr)
Noseda R,Guerrero-Valero M,Alberizzi V,Previtali SC,Sherman DL,Palmisano M,Huganir RL,Nave KA,Cuenda A,Feltri ML,Brophy PJ,Bolino A

A Critical Role for the GluA1 Accessory Protein, SAP97, in Cocaine Seeking.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology41(3)736-50(2016 Feb)
White SL,Ortinski PI,Friedman SH,Zhang L,Neve RL,Kalb RG,Schmidt HD,Pierce RC

Structure-function analysis of SAP97, a modular scaffolding protein that drives dendrite growth.
Molecular and cellular neurosciences6531-44(2015 Mar)
Zhang L,Hsu FC,Mojsilovic-Petrovic J,Jablonski AM,Zhai J,Coulter DA,Kalb RG

Corticotropin-Releasing Hormone Receptor Type 1 (CRHR1) Clustering with MAGUKs Is Mediated via Its C-Terminal PDZ Binding Motif.
PloS one10(9)e0136768(2015)
Bender J,Engeholm M,Ederer MS,Breu J,M?ller TC,Michalakis S,Rasko T,Wanker EE,Biel M,Martinez KL,Wurst W,Deussing JM

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Dlg1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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