Cdkn2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdkn2a
Entrez Gene ID	25163
Full Name	cyclin-dependent kinase inhibitor 2A
Synonyms	Arf,INK4A,MTS1,p16,p16Cdkn2a,p19ARF
General protein information	Preferred Names cyclin-dependent kinase inhibitor 2A Names cyclin-dependent kinase inhibitor 2A CDK4I Cyclin dependent kinase inhibitor 2A (p16, inhibits CDK4) alternative reading frame cdkn2a cell cycle inhibitor cell cycle regulator cyclin-dependent kinase 4 inhibitor A cyclin-dependent kinase inhibitor 2a p16Ink4a cyclin-dependent kinase inhibitor 2a p19Arf p16-INK4 p16-INK4a
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q32
Summary	tumor suppressor that inhibits CDK4; inactivation or loss is associated with cellular transformation; may be involved in CNS degeneration following injury [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031550.1	NP_113738.1	cyclin-dependent kinase inhibitor 2A

Pathways from BioSystems

Homologies

	LOC709988	XP_001098554.1
Danio rerio (zebrafish)	LOC100329528	XP_002660514.1
Xenopus tropicalis (tropical clawed frog)	cdkn2b	NP_001006920.1
Canis lupus familiaris (dog)	CDKN2A	XP_003431646.2
Pan troglodytes (chimpanzee)	CDKN2A	NP_001139762.1
Bos taurus (cattle)	CDKN2A	XP_002689593.3
Mus musculus (house mouse)	Cdkn2a	NP_001035744.1
Rattus norvegicus (Norway rat)	Cdkn2a	NP_113738.1
Homo sapiens (human)	CDKN2A	NP_000068.1

Gene Ontology
Bibliography

Related articles in PubMed

5-aza-2'-deoxycytidine, a DNA methylation inhibitor, attenuates hyperoxia-induced lung fibrosis via re-expression of P16 in neonatal rats.
Zhao SM, Wu HM, Cao ML, Han D
Pediatric research(2017 Dec)

GLTSCR2 promotes the nucleoplasmic translocation and subsequent degradation of nucleolar ARF.
Lee S, Cho YE, Kim SH, Kim YJ, Park JH
Oncotarget8(10)16293-16302(2017 Mar)

Inherited coding variants at the CDKN2A locus influence susceptibility to acute lymphoblastic leukaemia in children.
Xu H, Zhang H, Yang W, Yadav R, Morrison AC, Qian M, Devidas M, Liu Y, Perez-Andreu V, Zhao X, Gastier-Foster JM, Lupo PJ, Neale G, Raetz E, Larsen E, Bowman WP, Carroll WL, Winick N, Williams R, Hansen T, Holm JC, Mardis E, Fulton R, Pui CH, Zhang J, Mullighan CG, Evans WE, Hunger SP, Gupta R, Schmiegelow K, Loh ML, Relling MV, Yang JJ
Nature communications67553(2015 Jun)

Mutations in the p16 gene in DMBA-induced pancreatic intraepithelial neoplasia and pancreatic cancer in rats.
Zhu Z, Liu T, Han F, Zhan SD, Wang CY
Hepatobiliary & pancreatic diseases international : HBPD INT14(2)208-14(2015 Apr)

The p16(INK4A)/pRb pathway and telomerase activity define a subgroup of Ph+ adult Acute Lymphoblastic Leukemia associated with inferior outcome.
Chien WW, Catallo R, Chebel A, Baranger L, Thomas X, B?n? MC, Gerland LM, Schmidt A, Beldjord K, Klein N, Escoffre-Barbe M, Leguay T, Huguet F, Larosa F, Hayette S, Plesa A, Ifrah N, Dombret H, Salles G, Chassevent A, Ffrench M
Leukemia research39(4)453-61(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings indicated that p16 alteration is a common event in the carcinogenesis of this model and that the mutation pattern is analogous to that of human lesions.
Title: Mutations in the p16 gene in DMBA-induced pancreatic intraepithelial neoplasia and pancreatic cancer in rats.

These findings indicate that p16(INK4a) plays a significant role in the inhibition of DNA synthesis.
Title: Role of p16(INK4a) in the inhibition of DNA synthesis stimulated by HGF or EGF in primary cultured rat hepatocytes.

Ezh2 knockdown induced changes in DNA methylation of the p16INK4a gene and premature senescence in mesenchymal stem cells.
Title: H3K9me-enhanced DNA hypermethylation of the p16INK4a gene: an epigenetic signature for spontaneous transformation of rat mesenchymal stem cells.

Maternal high fat exposure represses p16 (INK4a) gene expression in the mammary gland of offspring through changes of histone modifications and HDAC3 binding activity within the regulatory regions of the p16 (INK4a) gene.
Title: Histone deacetylase 3 (HDAC3) participates in the transcriptional repression of the p16 (INK4a) gene in mammary gland of the female rat offspring exposed to an early-life high-fat diet.

Data show that an accumulation of p16(INK4a)-positive senescent astrocytes may link increased age and increased risk for sporadic Alzheimer's disease (AD).
Title: Astrocyte senescence as a component of Alzheimer's disease.

The following Cdkn2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdkn2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13193	NM_031550.1 Latest version!	Rattus norvegicus cyclin-dependent kinase inhibitor 2A (Cdkn2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdkn2a ( NM_031550.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113738.1 Rattus norvegicus cyclin-dependent kinase inhibitor 2A (Cdkn2a), mRNA.

Price & Availability↑

CloneID	ORa13193
Clone ID Related Accession (Same CDS sequence)	NM_031550.1
Accession Version	NM_031550.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Rattus norvegicus(Norway rat)
Product	cyclin-dependent kinase inhibitor 2A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L81167.1. On Dec 17, 2003 this sequence version replaced XM_346744.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L81167.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642727 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTCCT CTGCAGATAG ACTAGCCAGG GCAGCGGCCC TGGGCCGTGA GCACGAGGTG 
CGGGCACTGC TGGAAGCCGG GGCTTCACCA AACGCCCCGA ACACTTTCGG TCGTACCCCG 
ATACAGGTGA TGATGATGGG CAACGTCAAA GTGGCAGCTC TCCTGCTCTC CTATGGTGCA 
GATTCGAACT GCGAGGACCC CACCACCCTC TCCCGACCGG TGCACGACGC AGCGCGGGAG 
GGCTTCCTAG ACACTCTGGT AGTACTGCAC CAGGCAGGGG CGCGGCTGGA TGTGCGCGAT 
GCCTGGGGTC GCCTGCCGCT CGACCTGGCC CTAGAGCGGG GACATCACGA CGTCGTGCGG 
TATTTGCGGT ATCTACTCTC CTCCGCTGGG AACGTTTCCC GGGTCACCGA CAGGCATAAC 
TTCTGCTCAA GCACGCCCAG GTGCCTAGGA CTTCGAGGCC AACCCCCAAA GCAGCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113738.1
CDS	47..526
Translation	MESSADRLARAAALGREHEVRALLEAGASPNAPNTFGRTPIQVMMMGNVKVAALLLSYGADSNCEDPTTLSRPVHDAAREGFLDTLVVLHQAGARLDVRDAWGRLPLDLALERGHHDVVRYLRYLLSSAGNVSRVTDRHNFCSSTPRCLGLRGQPPKQR

Target ORF information:

RefSeq Version	NM_031550.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cyclin-dependent kinase inhibitor 2A (Cdkn2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031550.1

ORF Insert Sequence:

ATGGAGTCCT CTGCAGATAG ACTAGCCAGG GCAGCGGCCC TGGGCCGTGA GCACGAGGTG 
CGGGCACTGC TGGAAGCCGG GGCTTCACCA AACGCCCCGA ACACTTTCGG TCGTACCCCG 
ATACAGGTGA TGATGATGGG CAACGTCAAA GTGGCAGCTC TCCTGCTCTC CTATGGTGCA 
GATTCGAACT GCGAGGACCC CACCACCCTC TCCCGACCGG TGCACGACGC AGCGCGGGAG 
GGCTTCCTAG ACACTCTGGT AGTACTGCAC CAGGCAGGGG CGCGGCTGGA TGTGCGCGAT 
GCCTGGGGTC GCCTGCCGCT CGACCTGGCC CTAGAGCGGG GACATCACGA CGTCGTGCGG 
TATTTGCGGT ATCTACTCTC CTCCGCTGGG AACGTTTCCC GGGTCACCGA CAGGCATAAC 
TTCTGCTCAA GCACGCCCAG GTGCCTAGGA CTTCGAGGCC AACCCCCAAA GCAGCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

5-aza-2'-deoxycytidine, a DNA methylation inhibitor, attenuates hyperoxia-induced lung fibrosis via re-expression of P16 in neonatal rats.
Pediatric research(2017 Dec)
Zhao SM,Wu HM,Cao ML,Han D

GLTSCR2 promotes the nucleoplasmic translocation and subsequent degradation of nucleolar ARF.
Oncotarget8(10)16293-16302(2017 Mar)
Lee S,Cho YE,Kim SH,Kim YJ,Park JH

Inherited coding variants at the CDKN2A locus influence susceptibility to acute lymphoblastic leukaemia in children.
Nature communications67553(2015 Jun)
Xu H,Zhang H,Yang W,Yadav R,Morrison AC,Qian M,Devidas M,Liu Y,Perez-Andreu V,Zhao X,Gastier-Foster JM,Lupo PJ,Neale G,Raetz E,Larsen E,Bowman WP,Carroll WL,Winick N,Williams R,Hansen T,Holm JC,Mardis E,Fulton R,Pui CH,Zhang J,Mullighan CG,Evans WE,Hunger SP,Gupta R,Schmiegelow K,Loh ML,Relling MV,Yang JJ

Mutations in the p16 gene in DMBA-induced pancreatic intraepithelial neoplasia and pancreatic cancer in rats.
Hepatobiliary & pancreatic diseases international : HBPD INT14(2)208-14(2015 Apr)
Zhu Z,Liu T,Han F,Zhan SD,Wang CY

The p16(INK4A)/pRb pathway and telomerase activity define a subgroup of Ph+ adult Acute Lymphoblastic Leukemia associated with inferior outcome.
Leukemia research39(4)453-61(2015 Apr)
Chien WW,Catallo R,Chebel A,Baranger L,Thomas X,B?n? MC,Gerland LM,Schmidt A,Beldjord K,Klein N,Escoffre-Barbe M,Leguay T,Huguet F,Larosa F,Hayette S,Plesa A,Ifrah N,Dombret H,Salles G,Chassevent A,Ffrench M

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Cdkn2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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