Gnmt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gnmt
Entrez Gene ID	25134
Full Name	glycine N-methyltransferase
General protein information	Preferred Names glycine N-methyltransferase Names glycine N-methyltransferase folate-binding protein glycine methyltransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q12
Summary	catalyzes the conversion of S-adenosyl-L-methionine and glycine to S-adenosyl-L-homocysteine and sarcosine; involved in S-adenosylmethionine and folate metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017084.1	NP_058780.1	glycine N-methyltransferase

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gnmt	NP_058780.1
Rattus norvegicus (Norway rat)	LOC100911564	XP_006244568.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Proteomic investigation of signatures for geniposide-induced hepatotoxicity.
Wei J, Zhang F, Zhang Y, Cao C, Li X, Li D, Liu X, Yang H, Huang L
Journal of proteome research13(12)5724-33(2014 Dec)

S-adenosylmethionine in liver health, injury, and cancer.
Lu SC, Mato JM
Physiological reviews92(4)1515-42(2012 Oct)

A review of methionine dependency and the role of methionine restriction in cancer growth control and life-span extension.
Cavuoto P, Fenech MF
Cancer treatment reviews38(6)726-36(2012 Oct)

Differences in folate-protein interactions result in differing inhibition of native rat liver and recombinant glycine N-methyltransferase by 5-methyltetrahydrofolate.
Luka Z, Pakhomova S, Loukachevitch LV, Newcomer ME, Wagner C
Biochimica et biophysica acta1824(2)286-91(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that two biomarkers, glycine N-methyltransferase (GNMT) and glycogen phosphorylase (PYGL), were found to indicate early hepatic injuries.
Title: Proteomic investigation of signatures for geniposide-induced hepatotoxicity.

In the folate-GNMT complexes with the native enzyme, two folate molecules establish three and four hydrogen bonds with the protein. In the folate-recombinant GNMT complex only one hydrogen bond is established.
Title: Differences in folate-protein interactions result in differing inhibition of native rat liver and recombinant glycine N-methyltransferase by 5-methyltetrahydrofolate.

4S polycyclic aromatic hydrocarbon receptor (glycine N-methyltransferase) and the aryl hydrocarbon receptor nuclear translocator (hypoxia inducible factor-1beta) interaction in Chinese hamster ovary and rat hepatoma cells: 4S PAH-R/ARNT hetero-oligomers
Title: 4S polycyclic aromatic hydrocarbon receptor (glycine N-methyltransferase) and the aryl hydrocarbon receptor nuclear translocator (hypoxia inducible factor-1?) interaction in Chinese hamster ovary and rat hepatoma cells: 4S PAH-R/ARNT hetero-oligomers?

acetylation of the N-terminal valine affects several kinetic parameters of the enzyme
Title: Acetylation of N-terminal valine of glycine N-methyltransferase affects enzyme inhibition by folate.

First report of the crystal structure of rat GNMT complexed with its inhibitor, 5-methyltetrahydrofolate.
Title: 5-methyltetrahydrofolate is bound in intersubunit areas of rat liver folate-binding protein glycine N-methyltransferase.

The following Gnmt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gnmt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02765	NM_017084.1 Latest version!	Rattus norvegicus glycine N-methyltransferase (Gnmt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gnmt ( NM_017084.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058780.1 Rattus norvegicus glycine N-methyltransferase (Gnmt), mRNA.

Price & Availability↑

CloneID	ORa02765
Clone ID Related Accession (Same CDS sequence)	NM_017084.1
Accession Version	NM_017084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Rattus norvegicus(Norway rat)
Product	glycine N-methyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X06150.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X06150.1, EX491109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGACA GCGTGTACCG TACCCGCTCC CTGGGGGTGG CGGCCGAAGG GATCCCCGAC 
CAGTATGCGG ATGGGGAGGC CGCACGTGTG TGGCAGCTGT ACATCGGGGA CACCCGCAGC 
CGTACTGCAG AGTACAAGGC GTGGTTGCTT GGGCTGCTGC GCCAGCACGG GTGCCACCGG 
GTGCTGGACG TGGCCTGTGG CACAGGAGTG GACTCGATTA TGCTGGTGGA AGAGGGCTTT 
AGCGTCACGA GTGTGGATGC CAGCGACAAG ATGCTGAAAT ACGCACTGAA GGAGCGCTGG 
AACCGGAGGA AGGAGCCAGC CTTTGACAAG TGGGTCATTG AAGAAGCCAA CTGGTTGACT 
CTGGACAAAG ATGTGCCAGC AGGAGATGGC TTTGACGCTG TCATCTGCCT TGGGAACAGT 
TTTGCTCACC TGCCGGACAG CAAAGGTGAC CAGAGTGAGC ACCGGCTGGC GCTAAAGAAC 
ATCGCAAGCA TGGTGCGGCC CGGGGGCCTG CTGGTCATCG ACCACCGCAA CTACGACTAC 
ATCCTCAGCA CGGGCTGTGC ACCCCCAGGG AAGAACATCT ACTATAAGAG TGACCTGACC 
AAGGACATTA CGACGTCAGT GCTGACAGTA AACAACAAAG CCCACATGGT AACCCTGGAC 
TACACAGTGC AGGTGCCAGG TGCTGGCAGA GATGGCGCTC CTGGCTTCAG TAAGTTTCGG 
CTCTCTTACT ACCCACACTG TTTGGCGTCT TTCACGGAGT TGGTCCAAGA AGCCTTTGGG 
GGCAGGTGCC AGCACAGCGT CCTGGGTGAC TTCAAGCCTT ACAGGCCCGG CCAGGCCTAC 
GTTCCCTGCT ACTTCATCCA CGTGCTCAAG AAGACAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058780.1
CDS	5..886
Translation	MVDSVYRTRSLGVAAEGIPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCHRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRKEPAFDKWVIEEANWLTLDKDVPAGDGFDAVICLGNSFAHLPDSKGDQSEHRLALKNIASMVRPGGLLVIDHRNYDYILSTGCAPPGKNIYYKSDLTKDITTSVLTVNNKAHMVTLDYTVQVPGAGRDGAPGFSKFRLSYYPHCLASFTELVQEAFGGRCQHSVLGDFKPYRPGQAYVPCYFIHVLKKTG

Target ORF information:

RefSeq Version	NM_017084.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glycine N-methyltransferase (Gnmt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017084.1

ORF Insert Sequence:

ATGGTGGACA GCGTGTACCG TACCCGCTCC CTGGGGGTGG CGGCCGAAGG GATCCCCGAC 
CAGTATGCGG ATGGGGAGGC CGCACGTGTG TGGCAGCTGT ACATCGGGGA CACCCGCAGC 
CGTACTGCAG AGTACAAGGC GTGGTTGCTT GGGCTGCTGC GCCAGCACGG GTGCCACCGG 
GTGCTGGACG TGGCCTGTGG CACAGGAGTG GACTCGATTA TGCTGGTGGA AGAGGGCTTT 
AGCGTCACGA GTGTGGATGC CAGCGACAAG ATGCTGAAAT ACGCACTGAA GGAGCGCTGG 
AACCGGAGGA AGGAGCCAGC CTTTGACAAG TGGGTCATTG AAGAAGCCAA CTGGTTGACT 
CTGGACAAAG ATGTGCCAGC AGGAGATGGC TTTGACGCTG TCATCTGCCT TGGGAACAGT 
TTTGCTCACC TGCCGGACAG CAAAGGTGAC CAGAGTGAGC ACCGGCTGGC GCTAAAGAAC 
ATCGCAAGCA TGGTGCGGCC CGGGGGCCTG CTGGTCATCG ACCACCGCAA CTACGACTAC 
ATCCTCAGCA CGGGCTGTGC ACCCCCAGGG AAGAACATCT ACTATAAGAG TGACCTGACC 
AAGGACATTA CGACGTCAGT GCTGACAGTA AACAACAAAG CCCACATGGT AACCCTGGAC 
TACACAGTGC AGGTGCCAGG TGCTGGCAGA GATGGCGCTC CTGGCTTCAG TAAGTTTCGG 
CTCTCTTACT ACCCACACTG TTTGGCGTCT TTCACGGAGT TGGTCCAAGA AGCCTTTGGG 
GGCAGGTGCC AGCACAGCGT CCTGGGTGAC TTCAAGCCTT ACAGGCCCGG CCAGGCCTAC 
GTTCCCTGCT ACTTCATCCA CGTGCTCAAG AAGACAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Proteomic investigation of signatures for geniposide-induced hepatotoxicity.
Journal of proteome research13(12)5724-33(2014 Dec)
Wei J,Zhang F,Zhang Y,Cao C,Li X,Li D,Liu X,Yang H,Huang L

S-adenosylmethionine in liver health, injury, and cancer.
Physiological reviews92(4)1515-42(2012 Oct)
Lu SC,Mato JM

A review of methionine dependency and the role of methionine restriction in cancer growth control and life-span extension.
Cancer treatment reviews38(6)726-36(2012 Oct)
Cavuoto P,Fenech MF

Differences in folate-protein interactions result in differing inhibition of native rat liver and recombinant glycine N-methyltransferase by 5-methyltetrahydrofolate.
Biochimica et biophysica acta1824(2)286-91(2012 Feb)
Luka Z,Pakhomova S,Loukachevitch LV,Newcomer ME,Wagner C

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Gnmt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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