Vars cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Vars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa60041 XM_017601548.1
Latest version!
Rattus norvegicus valyl-tRNA synthetase (Vars), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa39212 NM_053292.1
Latest version!
Rattus norvegicus valyl-tRNA synthetase 1 (Vars1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
ORa39212 XM_006256047.3
Latest version!
Rattus norvegicus valyl-tRNA synthetase (Vars), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa60041
    Clone ID Related Accession (Same CDS sequence) XM_017601548.1
    Accession Version XM_017601548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3594bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product valine--tRNA ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTATTTGA CTTTTATTTT GTGTTTTGCC AGTGCACACG TGCGTGTATG TGTGTGTGTA 
    CATGTACGTA CACACACAAG TGTGTCTAGT GCTGAAGGAG TCTGGGTCCG TGTAACCTCT
    ATAACTGGGC TCACGATCTG TTGTGCCACC ACCACGTGGA TGCCAAGAAC CAAACTTGAG
    GCCTCTGCTG CCCTTGGGGC CCTGGGCAAG GCCTTGAACC CCTTGGAGGA ATGGCTTCGT
    CTGCACACCT ACCTGGCTGG GGACGCTCCC ACTCTGGCTG ACTTAGCTGC TGTGACAGCC
    TTACTGCTGC CCTTCCGATA CGTTCTGGAC CCTTCTGCCC GCCGGATCTG GGGTAATGTG
    ACTCGCTGGT TTAACACCTG TGTCCGGCAA CCAGAATTCC GGGCTGTGCT GGGAGAAGTG
    GTCCTGTACT CAGGGGCCAG GTCTGTCACT CAGCAGCCAG GTTCCGAGAT CACTGCACCC
    CAAAAGACAG CTGCTCAGCT CAAGAAAGAA GCAAAGAAAC GGGAGAAACT AGAGAAATTC
    CAACAGAAGC AGAAGACGCA GCAGCAGCAG CCGGCACACG GGGAGAAGAA ACCAAAACCA
    GAGAAGAAGG AGAAGCGAGA CCCCGGGGTC ATTACCTATG ACCTCCCTAC CCCACCTGGG
    GAGAAGAAAG ACGTAAGTGG CACCATGCCC GACTCCTACA GCCCTCAGTA TGTGGAGGCT
    GCCTGGTACC CTTGGTGGGA GCGGCAGGGC TTCTTCAAGC CAGAGTACGG GCGTCCTAGC
    GTGTCAGCAC CAAATCCCCG AGGTGTCTTC ATGATGTGCA TCCCACCCCC CAACGTGACA
    GGTTCCCTGC ACCTGGGCCA CGCGCTCACC AACGCCATCC AGGACTCCCT GACTCGATGG
    CACCGCATGC GGGGGGAGAC CACCCTATGG AACCCAGGCT GTGACCATGC TGGCATCGCC
    ACCCAGGTGG TGGTGGAAAA GAAACTCTGG AAAGAGCGGG GTCTGAACCG TCACCAGCTG
    GGCCGCGAGG CCTTTCTTCA GGAGGTCTGG AAGTGGAAAG CGGAGAAGGG TGACAGGATT
    TACCACCAAT TAAAGAAACT TGGCAGCTCC TTGGACTGGG ATCGAGCCTG CTTCACCATG
    GATCCAAAAT TGTCAGCAAC TGTGACAGAA GCTTTTGTTA GGCTCCATGA AGAAGGGGTC
    ATTTACCGCA GTACCCGCCT GGTCAACTGG TCCTGCACCC TCAACTCTGC CATTTCTGAC
    ATCGAGGTGG ATAAGAAAGA GCTGACGGGC CGCACCCTGC TCTCTGTGCC TGGCTACAAG
    GAGAAGGTGG AGTTTGGGGT CCTCGTGTCC TTTGCCTACA AGGTCCAAGG CTCAGACAGC
    GACGAGGAGG TGGTGGTGGC AACAACCCGG ATCGAGACAA TGCTGGGGGA CGTGGCTGTG
    GCTGTGCACC CCAAAGATCC CAGATACCAG CATTTAAAGG GAAAGAGTGT CGTCCACCCG
    TTCTTGTCCC GGAGCCTTCC CATTGTCTTT GATGACTTTG TGGACATGGA GTTTGGCACA
    GGTGCTGTGA AGATCACCCC GGCCCATGAC CAGAATGACT ATGAGGTTGG GCAGCGACAT
    AGGCTGGAGG CCATTAGCAT CATGGACTCG AAGGGGGCCC TTGTCAATGT GCCTCCACCT
    TTCCTGGGCC TGCCCAGGTT TGAGGCCAGG AAGGCTGTGC TGGCGGCCCT GAAGGAGCAG
    GGCCTGTTCC GCGGCATCAA GGACAACCCC ATGGTAGTGC CACTATGCAA CCGCTCCAAG
    GATGTGGTGG AGCCTCTGCT AAGGCCACAG TGGTACGTGC GCTGTGGGGA GATGGCTCAG
    GCCGCCAGTG CTGCCGTGAC CCGGGGAGAC CTCCGTATAC TGCCTGAGGC CCATCAGCGG
    ACGTGGCATT CTTGGATGGA CAACATCCGA GACTGGTGCA TCTCTCGGCA GCTGTGGTGG
    GGCCACCGAA TCCCTGCCTA CTTCATCACT GTCCATGACC CAGCGGTACC CCCTGGGGAG
    GACCCTGATG GGCGGTACTG GGTAAGCGGA CGCACTGAAG CAGAGGCCCG GGAGAAGGCA
    GCACGGGAAT TTGGAGTGTC CCCTGACAAG ATCAGCCTTC AGCAAGATGA GGATGTGTTA
    GACACCTGGT TCTCGTCTGG TCTCTTTCCC TTCTCCATCT TTGGCTGGCC CAATCAGTCA
    GAAGATCTCA GTGTGTTCTA CCCTGGGACC CTGCTGGAGA CGGGCCATGA TATCCTCTTC
    TTCTGGGTGG CGCGAATGGT CATGCTTGGC CTGAAGCTCA CTGAGAAGCT GCCCTTCAGA
    GAGGTCTACC TCCATGCAAT CGTGCGGGAC GCTCATGGCC GGAAGATGAG CAAGTCTCTC
    GGCAATGTCA TCGACCCCCT GGATGTCATC CATGGAGTTT CCTTGCAGGG CCTCCATGAC
    CAACTACTGA ACAGCAACCT GGATCCCAGT GAGGTGGAGA AAGCCAAAGA AGGACAGAGG
    GCCGACTTTC CAGCAGGGAT TCCCGAGTGT GGCACTGATG CCCTACGCTT TGGACTCTGT
    GCCTACACAT CCCAAGGTCG AGACATTAAC CTGGATGTGA ACAGGATCCT GGGGTACCGT
    CACTTCTGCA ACAAACTCTG GAATGCCACC AAGTTTGCTT TGCGCGGCCT TGGGAAGGGC
    TTTGTACCCT CACCAACCTC CAAGCCGGAA GGGCACGAGA GCCTGGTGGA CCGCTGGATC
    CGCAGCCGGC TGGCGGAGGC CGTGAGGCTC AGCAATGAAG GCTTCCAGGC TTATGATTTC
    CCAGCCGTCA CCACTGCCCA GTACAGCTTT TGGCTCTATG AGCTCTGTGA TGTCTACTTG
    GAGTGCCTAA AACCCGTTCT GAACGGAGTG GACCAGGTAG CAGCAGACTG TGCTCGGCAA
    ACCCTCTACA CCTGCCTGGA TGTCGGCCTG CGGCTGCTCT CACCCTTCAT GCCCTTTGTC
    ACAGAGGAGC TATTCCAGAG GCTGCCTCGG CGGACACCAA ACGCTCCTGC TAGCCTCTGT
    GTCACCCCCT ACCCAGAGCC CTCAGAGTGC TCCTGGAAGG ACCCTGAAGC CGAAGCTGCT
    CTTGAGCTAG CGCTGAGCAT CACTAGAGCT GTGCGCTCCC TGCGTGCAGA CTACAACCTG
    ACCCGGACCA GGCCCGACTG TTTCTTGGAA GTAGCTGATG AGGCTACGGG TGCCTTGGCC
    TCGGCAGTGT CGGCCTACGT GCAGACTCTG GCCAGTGCGG GTGTGGTGGC CGTCCTGGCC
    CTGGGTGCTC CCGCACCACA GGGCTGCGCT GTGGCTGTGG CCTCAGACCG CTGCTCCATC
    CACCTGCAGC TGCAGGGGCT CGTGGACCCA GCCCGGGAGC TGGGCAAGCT GCAGGCCAAG
    CGGAGTGAGG CACAGCGACA GGCTCAGCGG CTGCAGGAGC GCCGTGCTGC CTCTGGCTAC
    TCAGCAAAGG TGCCCCTTGA GGTCCAGGAG GCCGACGAAG TGAAGTTACA ACAGACAGAG
    GCGGAGCTCA GGAAGGTGGA TGAGGCCATC GCCCTGTTTC AGAAGATGCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017457037.1
    CDS1104..4697
    Translation

    Target ORF information:

    RefSeq Version XM_017601548.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus valyl-tRNA synthetase (Vars), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTATTTGA CTTTTATTTT GTGTTTTGCC AGTGCACACG TGCGTGTATG TGTGTGTGTA 
    CATGTACGTA CACACACAAG TGTGTCTAGT GCTGAAGGAG TCTGGGTCCG TGTAACCTCT
    ATAACTGGGC TCACGATCTG TTGTGCCACC ACCACGTGGA TGCCAAGAAC CAAACTTGAG
    GCCTCTGCTG CCCTTGGGGC CCTGGGCAAG GCCTTGAACC CCTTGGAGGA ATGGCTTCGT
    CTGCACACCT ACCTGGCTGG GGACGCTCCC ACTCTGGCTG ACTTAGCTGC TGTGACAGCC
    TTACTGCTGC CCTTCCGATA CGTTCTGGAC CCTTCTGCCC GCCGGATCTG GGGTAATGTG
    ACTCGCTGGT TTAACACCTG TGTCCGGCAA CCAGAATTCC GGGCTGTGCT GGGAGAAGTG
    GTCCTGTACT CAGGGGCCAG GTCTGTCACT CAGCAGCCAG GTTCCGAGAT CACTGCACCC
    CAAAAGACAG CTGCTCAGCT CAAGAAAGAA GCAAAGAAAC GGGAGAAACT AGAGAAATTC
    CAACAGAAGC AGAAGACGCA GCAGCAGCAG CCGGCACACG GGGAGAAGAA ACCAAAACCA
    GAGAAGAAGG AGAAGCGAGA CCCCGGGGTC ATTACCTATG ACCTCCCTAC CCCACCTGGG
    GAGAAGAAAG ACGTAAGTGG CACCATGCCC GACTCCTACA GCCCTCAGTA TGTGGAGGCT
    GCCTGGTACC CTTGGTGGGA GCGGCAGGGC TTCTTCAAGC CAGAGTACGG GCGTCCTAGC
    GTGTCAGCAC CAAATCCCCG AGGTGTCTTC ATGATGTGCA TCCCACCCCC CAACGTGACA
    GGTTCCCTGC ACCTGGGCCA CGCGCTCACC AACGCCATCC AGGACTCCCT GACTCGATGG
    CACCGCATGC GGGGGGAGAC CACCCTATGG AACCCAGGCT GTGACCATGC TGGCATCGCC
    ACCCAGGTGG TGGTGGAAAA GAAACTCTGG AAAGAGCGGG GTCTGAACCG TCACCAGCTG
    GGCCGCGAGG CCTTTCTTCA GGAGGTCTGG AAGTGGAAAG CGGAGAAGGG TGACAGGATT
    TACCACCAAT TAAAGAAACT TGGCAGCTCC TTGGACTGGG ATCGAGCCTG CTTCACCATG
    GATCCAAAAT TGTCAGCAAC TGTGACAGAA GCTTTTGTTA GGCTCCATGA AGAAGGGGTC
    ATTTACCGCA GTACCCGCCT GGTCAACTGG TCCTGCACCC TCAACTCTGC CATTTCTGAC
    ATCGAGGTGG ATAAGAAAGA GCTGACGGGC CGCACCCTGC TCTCTGTGCC TGGCTACAAG
    GAGAAGGTGG AGTTTGGGGT CCTCGTGTCC TTTGCCTACA AGGTCCAAGG CTCAGACAGC
    GACGAGGAGG TGGTGGTGGC AACAACCCGG ATCGAGACAA TGCTGGGGGA CGTGGCTGTG
    GCTGTGCACC CCAAAGATCC CAGATACCAG CATTTAAAGG GAAAGAGTGT CGTCCACCCG
    TTCTTGTCCC GGAGCCTTCC CATTGTCTTT GATGACTTTG TGGACATGGA GTTTGGCACA
    GGTGCTGTGA AGATCACCCC GGCCCATGAC CAGAATGACT ATGAGGTTGG GCAGCGACAT
    AGGCTGGAGG CCATTAGCAT CATGGACTCG AAGGGGGCCC TTGTCAATGT GCCTCCACCT
    TTCCTGGGCC TGCCCAGGTT TGAGGCCAGG AAGGCTGTGC TGGCGGCCCT GAAGGAGCAG
    GGCCTGTTCC GCGGCATCAA GGACAACCCC ATGGTAGTGC CACTATGCAA CCGCTCCAAG
    GATGTGGTGG AGCCTCTGCT AAGGCCACAG TGGTACGTGC GCTGTGGGGA GATGGCTCAG
    GCCGCCAGTG CTGCCGTGAC CCGGGGAGAC CTCCGTATAC TGCCTGAGGC CCATCAGCGG
    ACGTGGCATT CTTGGATGGA CAACATCCGA GACTGGTGCA TCTCTCGGCA GCTGTGGTGG
    GGCCACCGAA TCCCTGCCTA CTTCATCACT GTCCATGACC CAGCGGTACC CCCTGGGGAG
    GACCCTGATG GGCGGTACTG GGTAAGCGGA CGCACTGAAG CAGAGGCCCG GGAGAAGGCA
    GCACGGGAAT TTGGAGTGTC CCCTGACAAG ATCAGCCTTC AGCAAGATGA GGATGTGTTA
    GACACCTGGT TCTCGTCTGG TCTCTTTCCC TTCTCCATCT TTGGCTGGCC CAATCAGTCA
    GAAGATCTCA GTGTGTTCTA CCCTGGGACC CTGCTGGAGA CGGGCCATGA TATCCTCTTC
    TTCTGGGTGG CGCGAATGGT CATGCTTGGC CTGAAGCTCA CTGAGAAGCT GCCCTTCAGA
    GAGGTCTACC TCCATGCAAT CGTGCGGGAC GCTCATGGCC GGAAGATGAG CAAGTCTCTC
    GGCAATGTCA TCGACCCCCT GGATGTCATC CATGGAGTTT CCTTGCAGGG CCTCCATGAC
    CAACTACTGA ACAGCAACCT GGATCCCAGT GAGGTGGAGA AAGCCAAAGA AGGACAGAGG
    GCCGACTTTC CAGCAGGGAT TCCCGAGTGT GGCACTGATG CCCTACGCTT TGGACTCTGT
    GCCTACACAT CCCAAGGTCG AGACATTAAC CTGGATGTGA ACAGGATCCT GGGGTACCGT
    CACTTCTGCA ACAAACTCTG GAATGCCACC AAGTTTGCTT TGCGCGGCCT TGGGAAGGGC
    TTTGTACCCT CACCAACCTC CAAGCCGGAA GGGCACGAGA GCCTGGTGGA CCGCTGGATC
    CGCAGCCGGC TGGCGGAGGC CGTGAGGCTC AGCAATGAAG GCTTCCAGGC TTATGATTTC
    CCAGCCGTCA CCACTGCCCA GTACAGCTTT TGGCTCTATG AGCTCTGTGA TGTCTACTTG
    GAGTGCCTAA AACCCGTTCT GAACGGAGTG GACCAGGTAG CAGCAGACTG TGCTCGGCAA
    ACCCTCTACA CCTGCCTGGA TGTCGGCCTG CGGCTGCTCT CACCCTTCAT GCCCTTTGTC
    ACAGAGGAGC TATTCCAGAG GCTGCCTCGG CGGACACCAA ACGCTCCTGC TAGCCTCTGT
    GTCACCCCCT ACCCAGAGCC CTCAGAGTGC TCCTGGAAGG ACCCTGAAGC CGAAGCTGCT
    CTTGAGCTAG CGCTGAGCAT CACTAGAGCT GTGCGCTCCC TGCGTGCAGA CTACAACCTG
    ACCCGGACCA GGCCCGACTG TTTCTTGGAA GTAGCTGATG AGGCTACGGG TGCCTTGGCC
    TCGGCAGTGT CGGCCTACGT GCAGACTCTG GCCAGTGCGG GTGTGGTGGC CGTCCTGGCC
    CTGGGTGCTC CCGCACCACA GGGCTGCGCT GTGGCTGTGG CCTCAGACCG CTGCTCCATC
    CACCTGCAGC TGCAGGGGCT CGTGGACCCA GCCCGGGAGC TGGGCAAGCT GCAGGCCAAG
    CGGAGTGAGG CACAGCGACA GGCTCAGCGG CTGCAGGAGC GCCGTGCTGC CTCTGGCTAC
    TCAGCAAAGG TGCCCCTTGA GGTCCAGGAG GCCGACGAAG TGAAGTTACA ACAGACAGAG
    GCGGAGCTCA GGAAGGTGGA TGAGGCCATC GCCCTGTTTC AGAAGATGCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa39212
    Clone ID Related Accession (Same CDS sequence) NM_053292.1 , XM_006256047.3
    Accession Version NM_053292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-12
    Organism Rattus norvegicus(Norway rat)
    Product valine--tRNA ligase
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX883045.1. On Oct 13, 2007 this sequence version replaced XM_001076616.1. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCATCC TCTACGTCTC TCCCCACCCC GACGCCTTCC CCAGCCTGCG AGCCCTCATC 
    GCTGCCCGCT ACGGGGAAGC TGGGGACGGT CCCGGGTGGG GAGGCCCTCA CCCTCGCATC
    TGCCTGCAGC CTCCCCCGAG CAGCCGGACT CCATTCCCCC CGCCTCGCCT GCCCGCCCTG
    GAGCAGGGGC CTGGCGGCCT GTGGGTGTGG GGGGCCCCGG CTGTAGCTCA GCTGCTGTGG
    CCAGCAGGCC TGGGAGGACC CGGGGGCAGC CGGGCAGCTG TCCTGGTCCA GCAGTGGGTC
    AGTTATGCCG ACACGGAGCT CACACCAGCT GCCTGCGGGG CGACGCTGCC TGCTCTAGGA
    CTTCGAGGTC CTGGTCAGGA TCCCCAGGCT GCCCTTGGGG CCCTGGGCAA GGCCTTGAAC
    CCCTTGGAGG AATGGCTTCG TCTGCACACC TACCTGGCTG GGGACGCTCC CACTCTGGCT
    GACTTAGCTG CTGTGACAGC CTTACTGCTG CCCTTCCGAT ACGTTCTGGA CCCTTCTGCC
    CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTAACACCT GTGTCCGGCA ACCAGAATTC
    CGGGCTGTGC TGGGAGAAGT GGTCCTGTAC TCAGGGGCCA GGTCTGTCAC TCAGCAGCCA
    GGTTCCGAGA TCACTGCACC CCAAAAGACA GCTGCTCAGC TCAAGAAAGA AGCAAAGAAA
    CGGGAGAAAC TAGAGAAATT CCAACAGAAG CAGAAGACGC AGCAGCAGCA GCCGGCACAC
    GGGGAGAAGA AACCAAAACC AGAGAAGAAG GAGAAGCGAG ACCCCGGGGT CATTACCTAT
    GACCTCCCTA CCCCACCTGG GGAGAAGAAA GACGTAAGTG GCACCATGCC CGACTCCTAC
    AGCCCTCAGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCGGCAGGG CTTCTTCAAG
    CCAGAGTACG GGCGTCCTAG CGTGTCAGCA CCAAATCCCC GAGGTGTCTT CATGATGTGC
    ATCCCACCCC CCAACGTGAC AGGTTCCCTG CACCTGGGCC ACGCGCTCAC CAACGCCATC
    CAGGACTCCC TGACTCGATG GCACCGCATG CGGGGGGAGA CCACCCTATG GAACCCAGGC
    TGTGACCATG CTGGCATCGC CACCCAGGTG GTGGTGGAAA AGAAACTCTG GAAAGAGCGG
    GGTCTGAACC GTCACCAGCT GGGCCGCGAG GCCTTTCTTC AGGAGGTCTG GAAGTGGAAA
    GCGGAGAAGG GTGACAGGAT TTACCACCAA TTAAAGAAAC TTGGCAGCTC CTTGGACTGG
    GATCGAGCCT GCTTCACCAT GGATCCAAAA TTGTCAGCAA CTGTGACAGA AGCTTTTGTT
    AGGCTCCATG AAGAAGGGGT CATTTACCGC AGTACCCGCC TGGTCAACTG GTCCTGCACC
    CTCAACTCTG CCATTTCTGA CATCGAGGTG GATAAGAAAG AGCTGACGGG CCGCACCCTG
    CTCTCTGTGC CTGGCTACAA GGAGAAGGTG GAGTTTGGGG TCCTCGTGTC CTTTGCCTAC
    AAGGTCCAAG GCTCAGACAG CGACGAGGAG GTGGTGGTGG CAACAACCCG GATCGAGACA
    ATGCTGGGGG ACGTGGCTGT GGCTGTGCAC CCCAAAGATC CCAGATACCA GCATTTAAAG
    GGAAAGAGTG TCGTCCACCC GTTCTTGTCC CGGAGCCTTC CCATTGTCTT TGATGACTTT
    GTGGACATGG AGTTTGGCAC AGGTGCTGTG AAGATCACCC CGGCCCATGA CCAGAATGAC
    TATGAGGTTG GGCAGCGACA TAGGCTGGAG GCCATTAGCA TCATGGACTC GAAGGGGGCC
    CTTGTCAATG TGCCTCCACC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG GAAGGCTGTG
    CTGGCGGCCC TGAAGGAGCA GGGCCTGTTC CGCGGCATCA AGGACAACCC CATGGTAGTG
    CCACTATGCA ACCGCTCCAA GGATGTGGTG GAGCCTCTGC TAAGGCCACA GTGGTACGTG
    CGCTGTGGGG AGATGGCTCA GGCCGCCAGT GCTGCCGTGA CCCGGGGAGA CCTCCGTATA
    CTGCCTGAGG CCCATCAGCG GACGTGGCAT TCTTGGATGG ACAACATCCG AGACTGGTGC
    ATCTCTCGGC AGCTGTGGTG GGGCCACCGA ATCCCTGCCT ACTTCATCAC TGTCCATGAC
    CCAGCGGTAC CCCCTGGGGA GGACCCTGAT GGGCGGTACT GGGTAAGCGG ACGCACTGAA
    GCAGAGGCCC GGGAGAAGGC AGCACGGGAA TTTGGAGTGT CCCCTGACAA GATCAGCCTT
    CAGCAAGATG AGGATGTGTT AGACACCTGG TTCTCGTCTG GTCTCTTTCC CTTCTCCATC
    TTTGGCTGGC CCAATCAGTC AGAAGATCTC AGTGTGTTCT ACCCTGGGAC CCTGCTGGAG
    ACGGGCCATG ATATCCTCTT CTTCTGGGTG GCGCGAATGG TCATGCTTGG CCTGAAGCTC
    ACTGAGAAGC TGCCCTTCAG AGAGGTCTAC CTCCATGCAA TCGTGCGGGA CGCTCATGGC
    CGGAAGATGA GCAAGTCTCT CGGCAATGTC ATCGACCCCC TGGATGTCAT CCATGGAGTT
    TCCTTGCAGG GCCTCCATGA CCAACTACTG AACAGCAACC TGGATCCCAG TGAGGTGGAG
    AAAGCCAAAG AAGGACAGAG GGCCGACTTT CCAGCAGGGA TTCCCGAGTG TGGCACTGAT
    GCCCTACGCT TTGGACTCTG TGCCTACACA TCCCAAGGTC GAGACATTAA CCTGGATGTG
    AACAGGATCC TGGGGTACCG TCACTTCTGC AACAAACTCT GGAATGCCAC CAAGTTTGCT
    TTGCGCGGCC TTGGGAAGGG CTTTGTACCC TCACCAACCT CCAAGCCGGA AGGGCACGAG
    AGCCTGGTGG ACCGCTGGAT CCGCAGCCGG CTGGCGGAGG CCGTGAGGCT CAGCAATGAA
    GGCTTCCAGG CTTATGATTT CCCAGCCGTC ACCACTGCCC AGTACAGCTT TTGGCTCTAT
    GAGCTCTGTG ATGTCTACTT GGAGTGCCTA AAACCCGTTC TGAACGGAGT GGACCAGGTA
    GCAGCAGACT GTGCTCGGCA AACCCTCTAC ACCTGCCTGG ATGTCGGCCT GCGGCTGCTC
    TCACCCTTCA TGCCCTTTGT CACAGAGGAG CTATTCCAGA GGCTGCCTCG GCGGACACCA
    AACGCTCCTG CTAGCCTCTG TGTCACCCCC TACCCAGAGC CCTCAGAGTG CTCCTGGAAG
    GACCCTGAAG CCGAAGCTGC TCTTGAGCTA GCGCTGAGCA TCACTAGAGC TGTGCGCTCC
    CTGCGTGCAG ACTACAACCT GACCCGGACC AGGCCCGACT GTTTCTTGGA AGTAGCTGAT
    GAGGCTACGG GTGCCTTGGC CTCGGCAGTG TCGGCCTACG TGCAGACTCT GGCCAGTGCG
    GGTGTGGTGG CCGTCCTGGC CCTGGGTGCT CCCGCACCAC AGGGCTGCGC TGTGGCTGTG
    GCCTCAGACC GCTGCTCCAT CCACCTGCAG CTGCAGGGGC TCGTGGACCC AGCCCGGGAG
    CTGGGCAAGC TGCAGGCCAA GCGGAGTGAG GCACAGCGAC AGGCTCAGCG GCTGCAGGAG
    CGCCGTGCTG CCTCTGGCTA CTCAGCAAAG GTGCCCCTTG AGGTCCAGGA GGCCGACGAA
    GTGAAGTTAC AACAGACAGA GGCGGAGCTC AGGAAGGTGG ATGAGGCCAT CGCCCTGTTT
    CAGAAGATGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_445744.1
    CDS121..3915
    Translation

    Target ORF information:

    RefSeq Version NM_053292.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus valyl-tRNA synthetase 1 (Vars1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCATCC TCTACGTCTC TCCCCACCCC GACGCCTTCC CCAGCCTGCG AGCCCTCATC 
    GCTGCCCGCT ACGGGGAAGC TGGGGACGGT CCCGGGTGGG GAGGCCCTCA CCCTCGCATC
    TGCCTGCAGC CTCCCCCGAG CAGCCGGACT CCATTCCCCC CGCCTCGCCT GCCCGCCCTG
    GAGCAGGGGC CTGGCGGCCT GTGGGTGTGG GGGGCCCCGG CTGTAGCTCA GCTGCTGTGG
    CCAGCAGGCC TGGGAGGACC CGGGGGCAGC CGGGCAGCTG TCCTGGTCCA GCAGTGGGTC
    AGTTATGCCG ACACGGAGCT CACACCAGCT GCCTGCGGGG CGACGCTGCC TGCTCTAGGA
    CTTCGAGGTC CTGGTCAGGA TCCCCAGGCT GCCCTTGGGG CCCTGGGCAA GGCCTTGAAC
    CCCTTGGAGG AATGGCTTCG TCTGCACACC TACCTGGCTG GGGACGCTCC CACTCTGGCT
    GACTTAGCTG CTGTGACAGC CTTACTGCTG CCCTTCCGAT ACGTTCTGGA CCCTTCTGCC
    CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTAACACCT GTGTCCGGCA ACCAGAATTC
    CGGGCTGTGC TGGGAGAAGT GGTCCTGTAC TCAGGGGCCA GGTCTGTCAC TCAGCAGCCA
    GGTTCCGAGA TCACTGCACC CCAAAAGACA GCTGCTCAGC TCAAGAAAGA AGCAAAGAAA
    CGGGAGAAAC TAGAGAAATT CCAACAGAAG CAGAAGACGC AGCAGCAGCA GCCGGCACAC
    GGGGAGAAGA AACCAAAACC AGAGAAGAAG GAGAAGCGAG ACCCCGGGGT CATTACCTAT
    GACCTCCCTA CCCCACCTGG GGAGAAGAAA GACGTAAGTG GCACCATGCC CGACTCCTAC
    AGCCCTCAGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCGGCAGGG CTTCTTCAAG
    CCAGAGTACG GGCGTCCTAG CGTGTCAGCA CCAAATCCCC GAGGTGTCTT CATGATGTGC
    ATCCCACCCC CCAACGTGAC AGGTTCCCTG CACCTGGGCC ACGCGCTCAC CAACGCCATC
    CAGGACTCCC TGACTCGATG GCACCGCATG CGGGGGGAGA CCACCCTATG GAACCCAGGC
    TGTGACCATG CTGGCATCGC CACCCAGGTG GTGGTGGAAA AGAAACTCTG GAAAGAGCGG
    GGTCTGAACC GTCACCAGCT GGGCCGCGAG GCCTTTCTTC AGGAGGTCTG GAAGTGGAAA
    GCGGAGAAGG GTGACAGGAT TTACCACCAA TTAAAGAAAC TTGGCAGCTC CTTGGACTGG
    GATCGAGCCT GCTTCACCAT GGATCCAAAA TTGTCAGCAA CTGTGACAGA AGCTTTTGTT
    AGGCTCCATG AAGAAGGGGT CATTTACCGC AGTACCCGCC TGGTCAACTG GTCCTGCACC
    CTCAACTCTG CCATTTCTGA CATCGAGGTG GATAAGAAAG AGCTGACGGG CCGCACCCTG
    CTCTCTGTGC CTGGCTACAA GGAGAAGGTG GAGTTTGGGG TCCTCGTGTC CTTTGCCTAC
    AAGGTCCAAG GCTCAGACAG CGACGAGGAG GTGGTGGTGG CAACAACCCG GATCGAGACA
    ATGCTGGGGG ACGTGGCTGT GGCTGTGCAC CCCAAAGATC CCAGATACCA GCATTTAAAG
    GGAAAGAGTG TCGTCCACCC GTTCTTGTCC CGGAGCCTTC CCATTGTCTT TGATGACTTT
    GTGGACATGG AGTTTGGCAC AGGTGCTGTG AAGATCACCC CGGCCCATGA CCAGAATGAC
    TATGAGGTTG GGCAGCGACA TAGGCTGGAG GCCATTAGCA TCATGGACTC GAAGGGGGCC
    CTTGTCAATG TGCCTCCACC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG GAAGGCTGTG
    CTGGCGGCCC TGAAGGAGCA GGGCCTGTTC CGCGGCATCA AGGACAACCC CATGGTAGTG
    CCACTATGCA ACCGCTCCAA GGATGTGGTG GAGCCTCTGC TAAGGCCACA GTGGTACGTG
    CGCTGTGGGG AGATGGCTCA GGCCGCCAGT GCTGCCGTGA CCCGGGGAGA CCTCCGTATA
    CTGCCTGAGG CCCATCAGCG GACGTGGCAT TCTTGGATGG ACAACATCCG AGACTGGTGC
    ATCTCTCGGC AGCTGTGGTG GGGCCACCGA ATCCCTGCCT ACTTCATCAC TGTCCATGAC
    CCAGCGGTAC CCCCTGGGGA GGACCCTGAT GGGCGGTACT GGGTAAGCGG ACGCACTGAA
    GCAGAGGCCC GGGAGAAGGC AGCACGGGAA TTTGGAGTGT CCCCTGACAA GATCAGCCTT
    CAGCAAGATG AGGATGTGTT AGACACCTGG TTCTCGTCTG GTCTCTTTCC CTTCTCCATC
    TTTGGCTGGC CCAATCAGTC AGAAGATCTC AGTGTGTTCT ACCCTGGGAC CCTGCTGGAG
    ACGGGCCATG ATATCCTCTT CTTCTGGGTG GCGCGAATGG TCATGCTTGG CCTGAAGCTC
    ACTGAGAAGC TGCCCTTCAG AGAGGTCTAC CTCCATGCAA TCGTGCGGGA CGCTCATGGC
    CGGAAGATGA GCAAGTCTCT CGGCAATGTC ATCGACCCCC TGGATGTCAT CCATGGAGTT
    TCCTTGCAGG GCCTCCATGA CCAACTACTG AACAGCAACC TGGATCCCAG TGAGGTGGAG
    AAAGCCAAAG AAGGACAGAG GGCCGACTTT CCAGCAGGGA TTCCCGAGTG TGGCACTGAT
    GCCCTACGCT TTGGACTCTG TGCCTACACA TCCCAAGGTC GAGACATTAA CCTGGATGTG
    AACAGGATCC TGGGGTACCG TCACTTCTGC AACAAACTCT GGAATGCCAC CAAGTTTGCT
    TTGCGCGGCC TTGGGAAGGG CTTTGTACCC TCACCAACCT CCAAGCCGGA AGGGCACGAG
    AGCCTGGTGG ACCGCTGGAT CCGCAGCCGG CTGGCGGAGG CCGTGAGGCT CAGCAATGAA
    GGCTTCCAGG CTTATGATTT CCCAGCCGTC ACCACTGCCC AGTACAGCTT TTGGCTCTAT
    GAGCTCTGTG ATGTCTACTT GGAGTGCCTA AAACCCGTTC TGAACGGAGT GGACCAGGTA
    GCAGCAGACT GTGCTCGGCA AACCCTCTAC ACCTGCCTGG ATGTCGGCCT GCGGCTGCTC
    TCACCCTTCA TGCCCTTTGT CACAGAGGAG CTATTCCAGA GGCTGCCTCG GCGGACACCA
    AACGCTCCTG CTAGCCTCTG TGTCACCCCC TACCCAGAGC CCTCAGAGTG CTCCTGGAAG
    GACCCTGAAG CCGAAGCTGC TCTTGAGCTA GCGCTGAGCA TCACTAGAGC TGTGCGCTCC
    CTGCGTGCAG ACTACAACCT GACCCGGACC AGGCCCGACT GTTTCTTGGA AGTAGCTGAT
    GAGGCTACGG GTGCCTTGGC CTCGGCAGTG TCGGCCTACG TGCAGACTCT GGCCAGTGCG
    GGTGTGGTGG CCGTCCTGGC CCTGGGTGCT CCCGCACCAC AGGGCTGCGC TGTGGCTGTG
    GCCTCAGACC GCTGCTCCAT CCACCTGCAG CTGCAGGGGC TCGTGGACCC AGCCCGGGAG
    CTGGGCAAGC TGCAGGCCAA GCGGAGTGAG GCACAGCGAC AGGCTCAGCG GCTGCAGGAG
    CGCCGTGCTG CCTCTGGCTA CTCAGCAAAG GTGCCCCTTG AGGTCCAGGA GGCCGACGAA
    GTGAAGTTAC AACAGACAGA GGCGGAGCTC AGGAAGGTGG ATGAGGCCAT CGCCCTGTTT
    CAGAAGATGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa39212
    Clone ID Related Accession (Same CDS sequence) NM_053292.1 , XM_006256047.3
    Accession Version XM_006256047.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product valine--tRNA ligase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256047.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCATCC TCTACGTCTC TCCCCACCCC GACGCCTTCC CCAGCCTGCG AGCCCTCATC 
    GCTGCCCGCT ACGGGGAAGC TGGGGACGGT CCCGGGTGGG GAGGCCCTCA CCCTCGCATC
    TGCCTGCAGC CTCCCCCGAG CAGCCGGACT CCATTCCCCC CGCCTCGCCT GCCCGCCCTG
    GAGCAGGGGC CTGGCGGCCT GTGGGTGTGG GGGGCCCCGG CTGTAGCTCA GCTGCTGTGG
    CCAGCAGGCC TGGGAGGACC CGGGGGCAGC CGGGCAGCTG TCCTGGTCCA GCAGTGGGTC
    AGTTATGCCG ACACGGAGCT CACACCAGCT GCCTGCGGGG CGACGCTGCC TGCTCTAGGA
    CTTCGAGGTC CTGGTCAGGA TCCCCAGGCT GCCCTTGGGG CCCTGGGCAA GGCCTTGAAC
    CCCTTGGAGG AATGGCTTCG TCTGCACACC TACCTGGCTG GGGACGCTCC CACTCTGGCT
    GACTTAGCTG CTGTGACAGC CTTACTGCTG CCCTTCCGAT ACGTTCTGGA CCCTTCTGCC
    CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTAACACCT GTGTCCGGCA ACCAGAATTC
    CGGGCTGTGC TGGGAGAAGT GGTCCTGTAC TCAGGGGCCA GGTCTGTCAC TCAGCAGCCA
    GGTTCCGAGA TCACTGCACC CCAAAAGACA GCTGCTCAGC TCAAGAAAGA AGCAAAGAAA
    CGGGAGAAAC TAGAGAAATT CCAACAGAAG CAGAAGACGC AGCAGCAGCA GCCGGCACAC
    GGGGAGAAGA AACCAAAACC AGAGAAGAAG GAGAAGCGAG ACCCCGGGGT CATTACCTAT
    GACCTCCCTA CCCCACCTGG GGAGAAGAAA GACGTAAGTG GCACCATGCC CGACTCCTAC
    AGCCCTCAGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCGGCAGGG CTTCTTCAAG
    CCAGAGTACG GGCGTCCTAG CGTGTCAGCA CCAAATCCCC GAGGTGTCTT CATGATGTGC
    ATCCCACCCC CCAACGTGAC AGGTTCCCTG CACCTGGGCC ACGCGCTCAC CAACGCCATC
    CAGGACTCCC TGACTCGATG GCACCGCATG CGGGGGGAGA CCACCCTATG GAACCCAGGC
    TGTGACCATG CTGGCATCGC CACCCAGGTG GTGGTGGAAA AGAAACTCTG GAAAGAGCGG
    GGTCTGAACC GTCACCAGCT GGGCCGCGAG GCCTTTCTTC AGGAGGTCTG GAAGTGGAAA
    GCGGAGAAGG GTGACAGGAT TTACCACCAA TTAAAGAAAC TTGGCAGCTC CTTGGACTGG
    GATCGAGCCT GCTTCACCAT GGATCCAAAA TTGTCAGCAA CTGTGACAGA AGCTTTTGTT
    AGGCTCCATG AAGAAGGGGT CATTTACCGC AGTACCCGCC TGGTCAACTG GTCCTGCACC
    CTCAACTCTG CCATTTCTGA CATCGAGGTG GATAAGAAAG AGCTGACGGG CCGCACCCTG
    CTCTCTGTGC CTGGCTACAA GGAGAAGGTG GAGTTTGGGG TCCTCGTGTC CTTTGCCTAC
    AAGGTCCAAG GCTCAGACAG CGACGAGGAG GTGGTGGTGG CAACAACCCG GATCGAGACA
    ATGCTGGGGG ACGTGGCTGT GGCTGTGCAC CCCAAAGATC CCAGATACCA GCATTTAAAG
    GGAAAGAGTG TCGTCCACCC GTTCTTGTCC CGGAGCCTTC CCATTGTCTT TGATGACTTT
    GTGGACATGG AGTTTGGCAC AGGTGCTGTG AAGATCACCC CGGCCCATGA CCAGAATGAC
    TATGAGGTTG GGCAGCGACA TAGGCTGGAG GCCATTAGCA TCATGGACTC GAAGGGGGCC
    CTTGTCAATG TGCCTCCACC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG GAAGGCTGTG
    CTGGCGGCCC TGAAGGAGCA GGGCCTGTTC CGCGGCATCA AGGACAACCC CATGGTAGTG
    CCACTATGCA ACCGCTCCAA GGATGTGGTG GAGCCTCTGC TAAGGCCACA GTGGTACGTG
    CGCTGTGGGG AGATGGCTCA GGCCGCCAGT GCTGCCGTGA CCCGGGGAGA CCTCCGTATA
    CTGCCTGAGG CCCATCAGCG GACGTGGCAT TCTTGGATGG ACAACATCCG AGACTGGTGC
    ATCTCTCGGC AGCTGTGGTG GGGCCACCGA ATCCCTGCCT ACTTCATCAC TGTCCATGAC
    CCAGCGGTAC CCCCTGGGGA GGACCCTGAT GGGCGGTACT GGGTAAGCGG ACGCACTGAA
    GCAGAGGCCC GGGAGAAGGC AGCACGGGAA TTTGGAGTGT CCCCTGACAA GATCAGCCTT
    CAGCAAGATG AGGATGTGTT AGACACCTGG TTCTCGTCTG GTCTCTTTCC CTTCTCCATC
    TTTGGCTGGC CCAATCAGTC AGAAGATCTC AGTGTGTTCT ACCCTGGGAC CCTGCTGGAG
    ACGGGCCATG ATATCCTCTT CTTCTGGGTG GCGCGAATGG TCATGCTTGG CCTGAAGCTC
    ACTGAGAAGC TGCCCTTCAG AGAGGTCTAC CTCCATGCAA TCGTGCGGGA CGCTCATGGC
    CGGAAGATGA GCAAGTCTCT CGGCAATGTC ATCGACCCCC TGGATGTCAT CCATGGAGTT
    TCCTTGCAGG GCCTCCATGA CCAACTACTG AACAGCAACC TGGATCCCAG TGAGGTGGAG
    AAAGCCAAAG AAGGACAGAG GGCCGACTTT CCAGCAGGGA TTCCCGAGTG TGGCACTGAT
    GCCCTACGCT TTGGACTCTG TGCCTACACA TCCCAAGGTC GAGACATTAA CCTGGATGTG
    AACAGGATCC TGGGGTACCG TCACTTCTGC AACAAACTCT GGAATGCCAC CAAGTTTGCT
    TTGCGCGGCC TTGGGAAGGG CTTTGTACCC TCACCAACCT CCAAGCCGGA AGGGCACGAG
    AGCCTGGTGG ACCGCTGGAT CCGCAGCCGG CTGGCGGAGG CCGTGAGGCT CAGCAATGAA
    GGCTTCCAGG CTTATGATTT CCCAGCCGTC ACCACTGCCC AGTACAGCTT TTGGCTCTAT
    GAGCTCTGTG ATGTCTACTT GGAGTGCCTA AAACCCGTTC TGAACGGAGT GGACCAGGTA
    GCAGCAGACT GTGCTCGGCA AACCCTCTAC ACCTGCCTGG ATGTCGGCCT GCGGCTGCTC
    TCACCCTTCA TGCCCTTTGT CACAGAGGAG CTATTCCAGA GGCTGCCTCG GCGGACACCA
    AACGCTCCTG CTAGCCTCTG TGTCACCCCC TACCCAGAGC CCTCAGAGTG CTCCTGGAAG
    GACCCTGAAG CCGAAGCTGC TCTTGAGCTA GCGCTGAGCA TCACTAGAGC TGTGCGCTCC
    CTGCGTGCAG ACTACAACCT GACCCGGACC AGGCCCGACT GTTTCTTGGA AGTAGCTGAT
    GAGGCTACGG GTGCCTTGGC CTCGGCAGTG TCGGCCTACG TGCAGACTCT GGCCAGTGCG
    GGTGTGGTGG CCGTCCTGGC CCTGGGTGCT CCCGCACCAC AGGGCTGCGC TGTGGCTGTG
    GCCTCAGACC GCTGCTCCAT CCACCTGCAG CTGCAGGGGC TCGTGGACCC AGCCCGGGAG
    CTGGGCAAGC TGCAGGCCAA GCGGAGTGAG GCACAGCGAC AGGCTCAGCG GCTGCAGGAG
    CGCCGTGCTG CCTCTGGCTA CTCAGCAAAG GTGCCCCTTG AGGTCCAGGA GGCCGACGAA
    GTGAAGTTAC AACAGACAGA GGCGGAGCTC AGGAAGGTGG ATGAGGCCAT CGCCCTGTTT
    CAGAAGATGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006256109.1
    CDS335..4129
    Translation

    Target ORF information:

    RefSeq Version XM_006256047.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus valyl-tRNA synthetase (Vars), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006256047.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCATCC TCTACGTCTC TCCCCACCCC GACGCCTTCC CCAGCCTGCG AGCCCTCATC 
    GCTGCCCGCT ACGGGGAAGC TGGGGACGGT CCCGGGTGGG GAGGCCCTCA CCCTCGCATC
    TGCCTGCAGC CTCCCCCGAG CAGCCGGACT CCATTCCCCC CGCCTCGCCT GCCCGCCCTG
    GAGCAGGGGC CTGGCGGCCT GTGGGTGTGG GGGGCCCCGG CTGTAGCTCA GCTGCTGTGG
    CCAGCAGGCC TGGGAGGACC CGGGGGCAGC CGGGCAGCTG TCCTGGTCCA GCAGTGGGTC
    AGTTATGCCG ACACGGAGCT CACACCAGCT GCCTGCGGGG CGACGCTGCC TGCTCTAGGA
    CTTCGAGGTC CTGGTCAGGA TCCCCAGGCT GCCCTTGGGG CCCTGGGCAA GGCCTTGAAC
    CCCTTGGAGG AATGGCTTCG TCTGCACACC TACCTGGCTG GGGACGCTCC CACTCTGGCT
    GACTTAGCTG CTGTGACAGC CTTACTGCTG CCCTTCCGAT ACGTTCTGGA CCCTTCTGCC
    CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTAACACCT GTGTCCGGCA ACCAGAATTC
    CGGGCTGTGC TGGGAGAAGT GGTCCTGTAC TCAGGGGCCA GGTCTGTCAC TCAGCAGCCA
    GGTTCCGAGA TCACTGCACC CCAAAAGACA GCTGCTCAGC TCAAGAAAGA AGCAAAGAAA
    CGGGAGAAAC TAGAGAAATT CCAACAGAAG CAGAAGACGC AGCAGCAGCA GCCGGCACAC
    GGGGAGAAGA AACCAAAACC AGAGAAGAAG GAGAAGCGAG ACCCCGGGGT CATTACCTAT
    GACCTCCCTA CCCCACCTGG GGAGAAGAAA GACGTAAGTG GCACCATGCC CGACTCCTAC
    AGCCCTCAGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCGGCAGGG CTTCTTCAAG
    CCAGAGTACG GGCGTCCTAG CGTGTCAGCA CCAAATCCCC GAGGTGTCTT CATGATGTGC
    ATCCCACCCC CCAACGTGAC AGGTTCCCTG CACCTGGGCC ACGCGCTCAC CAACGCCATC
    CAGGACTCCC TGACTCGATG GCACCGCATG CGGGGGGAGA CCACCCTATG GAACCCAGGC
    TGTGACCATG CTGGCATCGC CACCCAGGTG GTGGTGGAAA AGAAACTCTG GAAAGAGCGG
    GGTCTGAACC GTCACCAGCT GGGCCGCGAG GCCTTTCTTC AGGAGGTCTG GAAGTGGAAA
    GCGGAGAAGG GTGACAGGAT TTACCACCAA TTAAAGAAAC TTGGCAGCTC CTTGGACTGG
    GATCGAGCCT GCTTCACCAT GGATCCAAAA TTGTCAGCAA CTGTGACAGA AGCTTTTGTT
    AGGCTCCATG AAGAAGGGGT CATTTACCGC AGTACCCGCC TGGTCAACTG GTCCTGCACC
    CTCAACTCTG CCATTTCTGA CATCGAGGTG GATAAGAAAG AGCTGACGGG CCGCACCCTG
    CTCTCTGTGC CTGGCTACAA GGAGAAGGTG GAGTTTGGGG TCCTCGTGTC CTTTGCCTAC
    AAGGTCCAAG GCTCAGACAG CGACGAGGAG GTGGTGGTGG CAACAACCCG GATCGAGACA
    ATGCTGGGGG ACGTGGCTGT GGCTGTGCAC CCCAAAGATC CCAGATACCA GCATTTAAAG
    GGAAAGAGTG TCGTCCACCC GTTCTTGTCC CGGAGCCTTC CCATTGTCTT TGATGACTTT
    GTGGACATGG AGTTTGGCAC AGGTGCTGTG AAGATCACCC CGGCCCATGA CCAGAATGAC
    TATGAGGTTG GGCAGCGACA TAGGCTGGAG GCCATTAGCA TCATGGACTC GAAGGGGGCC
    CTTGTCAATG TGCCTCCACC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG GAAGGCTGTG
    CTGGCGGCCC TGAAGGAGCA GGGCCTGTTC CGCGGCATCA AGGACAACCC CATGGTAGTG
    CCACTATGCA ACCGCTCCAA GGATGTGGTG GAGCCTCTGC TAAGGCCACA GTGGTACGTG
    CGCTGTGGGG AGATGGCTCA GGCCGCCAGT GCTGCCGTGA CCCGGGGAGA CCTCCGTATA
    CTGCCTGAGG CCCATCAGCG GACGTGGCAT TCTTGGATGG ACAACATCCG AGACTGGTGC
    ATCTCTCGGC AGCTGTGGTG GGGCCACCGA ATCCCTGCCT ACTTCATCAC TGTCCATGAC
    CCAGCGGTAC CCCCTGGGGA GGACCCTGAT GGGCGGTACT GGGTAAGCGG ACGCACTGAA
    GCAGAGGCCC GGGAGAAGGC AGCACGGGAA TTTGGAGTGT CCCCTGACAA GATCAGCCTT
    CAGCAAGATG AGGATGTGTT AGACACCTGG TTCTCGTCTG GTCTCTTTCC CTTCTCCATC
    TTTGGCTGGC CCAATCAGTC AGAAGATCTC AGTGTGTTCT ACCCTGGGAC CCTGCTGGAG
    ACGGGCCATG ATATCCTCTT CTTCTGGGTG GCGCGAATGG TCATGCTTGG CCTGAAGCTC
    ACTGAGAAGC TGCCCTTCAG AGAGGTCTAC CTCCATGCAA TCGTGCGGGA CGCTCATGGC
    CGGAAGATGA GCAAGTCTCT CGGCAATGTC ATCGACCCCC TGGATGTCAT CCATGGAGTT
    TCCTTGCAGG GCCTCCATGA CCAACTACTG AACAGCAACC TGGATCCCAG TGAGGTGGAG
    AAAGCCAAAG AAGGACAGAG GGCCGACTTT CCAGCAGGGA TTCCCGAGTG TGGCACTGAT
    GCCCTACGCT TTGGACTCTG TGCCTACACA TCCCAAGGTC GAGACATTAA CCTGGATGTG
    AACAGGATCC TGGGGTACCG TCACTTCTGC AACAAACTCT GGAATGCCAC CAAGTTTGCT
    TTGCGCGGCC TTGGGAAGGG CTTTGTACCC TCACCAACCT CCAAGCCGGA AGGGCACGAG
    AGCCTGGTGG ACCGCTGGAT CCGCAGCCGG CTGGCGGAGG CCGTGAGGCT CAGCAATGAA
    GGCTTCCAGG CTTATGATTT CCCAGCCGTC ACCACTGCCC AGTACAGCTT TTGGCTCTAT
    GAGCTCTGTG ATGTCTACTT GGAGTGCCTA AAACCCGTTC TGAACGGAGT GGACCAGGTA
    GCAGCAGACT GTGCTCGGCA AACCCTCTAC ACCTGCCTGG ATGTCGGCCT GCGGCTGCTC
    TCACCCTTCA TGCCCTTTGT CACAGAGGAG CTATTCCAGA GGCTGCCTCG GCGGACACCA
    AACGCTCCTG CTAGCCTCTG TGTCACCCCC TACCCAGAGC CCTCAGAGTG CTCCTGGAAG
    GACCCTGAAG CCGAAGCTGC TCTTGAGCTA GCGCTGAGCA TCACTAGAGC TGTGCGCTCC
    CTGCGTGCAG ACTACAACCT GACCCGGACC AGGCCCGACT GTTTCTTGGA AGTAGCTGAT
    GAGGCTACGG GTGCCTTGGC CTCGGCAGTG TCGGCCTACG TGCAGACTCT GGCCAGTGCG
    GGTGTGGTGG CCGTCCTGGC CCTGGGTGCT CCCGCACCAC AGGGCTGCGC TGTGGCTGTG
    GCCTCAGACC GCTGCTCCAT CCACCTGCAG CTGCAGGGGC TCGTGGACCC AGCCCGGGAG
    CTGGGCAAGC TGCAGGCCAA GCGGAGTGAG GCACAGCGAC AGGCTCAGCG GCTGCAGGAG
    CGCCGTGCTG CCTCTGGCTA CTCAGCAAAG GTGCCCCTTG AGGTCCAGGA GGCCGACGAA
    GTGAAGTTAC AACAGACAGA GGCGGAGCTC AGGAAGGTGG ATGAGGCCAT CGCCCTGTTT
    CAGAAGATGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.