Spink3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spink3
Entrez Gene ID	24833
Full Name	serine peptidase inhibitor, Kazal type 3
Synonyms	Psti-1,Spink1,Tilp
General protein information	Preferred Names serine peptidase inhibitor, Kazal type 3 Names serine protease inhibitor Kazal-type 1 PSTI-I Serine protease inhibitor, kanzal type 1/ Trypsin inhibitor-like protein, pancreatic cholecystokinin-releasing peptide monitor peptide pancreatic cholecystokinin(CCK) releasing peptide pancreatic secretory trypsin inhibitor I trypsin inhibitor-like protein, pancreatic
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q11
Summary	acts as a trypsin inhibitor to protect the pancreas from premature activation of pancreatic juice; has additional monitor peptide activity to induce cholecystokinin release in the intestine [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012674.2	NP_036806.1	serine protease inhibitor Kazal-type 1 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Spink3	NP_036806.1
Rattus norvegicus (Norway rat)	Spink1	NP_690919.1

Gene Ontology
Bibliography

Related articles in PubMed

[SPINK3: A novel growth factor that promotes rat liver regeneration].
Chang CF, Yang J, Li XF, Zhao WM, Chen SS, Wang GP, Xu CS
Molekuliarnaia biologiia50(3)457-65(2016 May-Jun)

SPINK3 modulates mouse sperm physiology through the reduction of nitric oxide level independently of its trypsin inhibitory activity.
Zalazar L, Saez Lancellotti TE, Clementi M, Lombardo C, Lamattina L, De Castro R, Forn?s MW, Cesari A
Reproduction (Cambridge, England)143(3)281-95(2012 Mar)

The striatum is highly susceptible to mitochondrial oxidative phosphorylation dysfunctions.
Pickrell AM, Fukui H, Wang X, Pinto M, Moraes CT
The Journal of neuroscience : the official journal of the Society for Neuroscience31(27)9895-904(2011 Jul)

Transgenic expression of pancreatic secretory trypsin inhibitor-1 rescues SPINK3-deficient mice and restores a normal pancreatic phenotype.
Romac JM, Ohmuraya M, Bittner C, Majeed MF, Vigna SR, Que J, Fee BE, Wartmann T, Yamamura K, Liddle RA
American journal of physiology. Gastrointestinal and liver physiology298(4)G518-24(2010 Apr)

Protection against chronic pancreatitis and pancreatic fibrosis in mice overexpressing pancreatic secretory trypsin inhibitor.
Nathan JD, Romac J, Peng RY, Peyton M, Rockey DC, Liddle RA
Pancreas39(1)e24-30(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPINK3 along with Ccna2 and EGFR accelerated cell proliferation during rat liver regeneration via P38 MAPK, PKC, JAK-STAT and AKT pathways.
Title: [SPINK3: A novel growth factor that promotes rat liver regeneration].

This study created a novel mouse model of mtDNA depletion by expressing relatively low levels of mito-PstI in CNS neurons, which leads to a chronic OXPHOS deficiency.
Title: The striatum is highly susceptible to mitochondrial oxidative phosphorylation dysfunctions.

severity of chronic pancreatitis and pancreatic fibrosis is significantly reduced in mice expressing rat PSTI-I; propose that endogenous pancreatic trypsin inhibitors play a protective role in the pancreatic response to repeated injurious events
Title: Protection against chronic pancreatitis and pancreatic fibrosis in mice overexpressing pancreatic secretory trypsin inhibitor.

Caltrin protein modulates the acrosomal exocytosis during sperm capacitation.
Title: Rat caltrin protein modulates the acrosomal exocytosis during sperm capacitation.

Partially folded bovine pancreatic trypsin inhibitor analogues attain fully native structures when co-crystallized with S195A rat trypsin.
Title: Partially folded bovine pancreatic trypsin inhibitor analogues attain fully native structures when co-crystallized with S195A rat trypsin.

The following Spink3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spink3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00587	NM_012674.2 Latest version!	Rattus norvegicus serine peptidase inhibitor, Kazal type 3 (Spink3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spink3 ( NM_012674.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036806.1 Rattus norvegicus serine peptidase inhibitor, Kazal type 3 (Spink3), mRNA.

Price & Availability↑

CloneID	ORa00587
Clone ID Related Accession (Same CDS sequence)	NM_012674.2
Accession Version	NM_012674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	serine protease inhibitor Kazal-type 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X59696.1 and M27882.1. On Oct 19, 2007 this sequence version replaced NM_012674.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X59696.1, M35299.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598, SAMN01906355 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTAG CAATTATCTT TCTTCTCAGT GCTTTGGCCC TGCTCAGTTT AGCAGGTAAC 
CCTCCAGCTG AGGTGAATGG AAAAACGCCT AATTGCCCTA AGCAAATTAT GGGATGTCCC 
AGGATTTATG ACCCTGTGTG TGGGACTAAC GGAATTACTT ACCCCAGTGA ATGCAGTCTG 
TGCTTTGAAA ACAGGAAATT CGGAACATCT ATCCACATTC AGAGGAGAGG GACTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036806.1
CDS	61..300
Translation	MKVAIIFLLSALALLSLAGNPPAEVNGKTPNCPKQIMGCPRIYDPVCGTNGITYPSECSLCFENRKFGTSIHIQRRGTC

Target ORF information:

RefSeq Version	NM_012674.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine peptidase inhibitor, Kazal type 3 (Spink3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012674.2

ORF Insert Sequence:

ATGAAGGTAG CAATTATCTT TCTTCTCAGT GCTTTGGCCC TGCTCAGTTT AGCAGGTAAC 
CCTCCAGCTG AGGTGAATGG AAAAACGCCT AATTGCCCTA AGCAAATTAT GGGATGTCCC 
AGGATTTATG ACCCTGTGTG TGGGACTAAC GGAATTACTT ACCCCAGTGA ATGCAGTCTG 
TGCTTTGAAA ACAGGAAATT CGGAACATCT ATCCACATTC AGAGGAGAGG GACTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[SPINK3: A novel growth factor that promotes rat liver regeneration].
Molekuliarnaia biologiia50(3)457-65(2016 May-Jun)
Chang CF,Yang J,Li XF,Zhao WM,Chen SS,Wang GP,Xu CS

SPINK3 modulates mouse sperm physiology through the reduction of nitric oxide level independently of its trypsin inhibitory activity.
Reproduction (Cambridge, England)143(3)281-95(2012 Mar)
Zalazar L,Saez Lancellotti TE,Clementi M,Lombardo C,Lamattina L,De Castro R,Forn?s MW,Cesari A

The striatum is highly susceptible to mitochondrial oxidative phosphorylation dysfunctions.
The Journal of neuroscience : the official journal of the Society for Neuroscience31(27)9895-904(2011 Jul)
Pickrell AM,Fukui H,Wang X,Pinto M,Moraes CT

Transgenic expression of pancreatic secretory trypsin inhibitor-1 rescues SPINK3-deficient mice and restores a normal pancreatic phenotype.
American journal of physiology. Gastrointestinal and liver physiology298(4)G518-24(2010 Apr)
Romac JM,Ohmuraya M,Bittner C,Majeed MF,Vigna SR,Que J,Fee BE,Wartmann T,Yamamura K,Liddle RA

Protection against chronic pancreatitis and pancreatic fibrosis in mice overexpressing pancreatic secretory trypsin inhibitor.
Pancreas39(1)e24-30(2010 Jan)
Nathan JD,Romac J,Peng RY,Peyton M,Rockey DC,Liddle RA