Gtf3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gtf3a
Entrez Gene ID	246299
Full Name	general transcription factor III A
General protein information	Preferred Names general transcription factor III A Names transcription factor IIIA TFIIIA factor A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12p11
Summary	The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113570.1	NP_001107042.1	transcription factor IIIA

Pathways from BioSystems

Homologies

Homo sapiens (human)	GTF3A	NP_002088.2
Mus musculus (house mouse)	Gtf3a	NP_079928.2
Rattus norvegicus (Norway rat)	Gtf3a	NP_001107042.1
Gallus gallus (chicken)	GTF3A	NP_001107043.1
Drosophila melanogaster (fruit fly)	CG9609	NP_573161.1
Magnaporthe oryzae (rice blast fungus)	MGG_05322	XP_003712886.1
Pan troglodytes (chimpanzee)	GTF3A	XP_522641.3
	GTF3A	XP_001095556.1
Canis lupus familiaris (dog)	GTF3A	XP_534523.3
Bos taurus (cattle)	GTF3A	NP_001107044.1
Danio rerio (zebrafish)	gtf3aa	NP_001003866.1
Schizosaccharomyces pombe (fission yeast)	sfc2	NP_594670.1
Xenopus tropicalis (tropical clawed frog)	gtf3a	XP_002941582.2

Gene Ontology
Bibliography

Related articles in PubMed

The 5S RNP couples p53 homeostasis to ribosome biogenesis and nucleolar stress.
Sloan KE, Bohnsack MT, Watkins NJ
Cell reports5(1)237-47(2013 Oct)

A structural perspective on RNA polymerase I and RNA polymerase III transcription machineries.
Vannini A
Biochimica et biophysica acta1829(3-4)258-64(2013 Mar-Apr)

cDNA cloning, DNA binding, and evolution of mammalian transcription factor IIIA.
Hanas JS, Hocker JR, Cheng YG, Lerner MR, Brackett DJ, Lightfoot SA, Hanas RJ, Madhusudhan KT, Moreland RJ
Gene282(1-2)43-52(2002 Jan)

Identification of a transcription factor IIIA-interacting protein.
Moreland RJ, Dresser ME, Rodgers JS, Roe BA, Conaway JW, Conaway RC, Hanas JS
Nucleic acids research28(9)1986-93(2000 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gtf3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gtf3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12037	NM_001113570.1 Latest version!	Rattus norvegicus general transcription factor III A (Gtf3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gtf3a ( NM_001113570.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001107042.1 Rattus norvegicus general transcription factor III A (Gtf3a), mRNA.

Price & Availability↑

CloneID	ORa12037
Clone ID Related Accession (Same CDS sequence)	NM_001113570.1
Accession Version	NM_001113570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor IIIA
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB616240.1, AF391798.1, CB578423.1 and CK842762.1. On Jan 25, 2008 this sequence version replaced XM_001069721.1. ##Evidence-Data-START## Transcript exon combination :: AF391798.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGAGCCGC GGGTCTCGGT CGCGGAAGCG GTGTCGTCCC TGACCATCGC GGACGCGTTC 
GTTCGGGCCT GTGTGGGCCC CGCCCCGCCG CGCCCCGCGC TCCCCAGCAG GTTCATCTGC 
TCCTTTCCTG ACTGCAGCGC TAGTTACAAC AAAGCCTGGA AGCTAGACGC GCACCTCTGC 
AAGCACACGG GGGAGAGGCC GTTCGTTTGT GACTATGAGG GCTGTGGCAA AGCCTTCATC 
AGGGACTACC ATCTGAGTCG ACACATCCTG ATTCACACCG GAGAAAAGCC ATTTGTTTGT 
GCAGATAATG GCTGTAATCA GAAATTCAGC ACAAAGTCAA ACTTGAAGAA ACACATTGAA 
CGCAAACATG AAAATCCACA AAAACAGTAT GTGTGCAATT TTGAAGGTTG CAAGAAGGCC 
TTTAAGAAGC ACCAGCAGCT GAGAACCCAT CAGTGCCAGC ACACAAATGA GCCACTCTTC 
AGGTGTACCC ACGAGGGATG TGGGAAGCAC TTTGCCTCCC CCAGCAGGCT GAAACGGCAT 
GGGAAGGTTC ACGAGGGCTA CCTATGTCAA AAGGGATGTT CCTTTGTGGG AAAAACGTGG 
ACAGAGCTTC TGAAGCACAC GAGAGAAGCC CATAAAGAGG AGGTGACCTG CACAGTGTGC 
CAGAAGATGT TCAAGCGCAA AGATCACCTT AAGCAGCACA TGAAGACTCA CGCTCCGGAG 
AGGGATGTGT ACCGCTGCCC GCGGGAAGGC TGTGCAAGGA CCTACACCAC CGTGTTCAAC 
CTGCAGAGCC ACATTCTCTC CTTCCACGAG GAAAAGCGCC CATTTGTGTG TGAGCACGCT 
GGCTGTGGCA AGACGTTTGC AATGAAACAA AGTCTCATGA GGCACAGTGT CGTGCACGAT 
CCTGACAAGA AGAGGATGAA GCTCAAAGTT AGACCACCTC GGGAGAGACG CAGCTTGGCC 
TCGCGCCTCA GCGGGTACGT CCCTCCTAAG GGGAAACAAG AGCCCGACTG CTCCTTGCCT 
AACAGCACAG AGTCCAGCAG CAGCCCAGAG GCCACGATGC TCGCCCCAGC TGCGTTACTT 
ACTGTCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107042.1
CDS	159..1250
Translation	LEPRVSVAEAVSSLTIADAFVRACVGPAPPRPALPSRFICSFPDCSASYNKAWKLDAHLCKHTGERPFVCDYEGCGKAFIRDYHLSRHILIHTGEKPFVCADNGCNQKFSTKSNLKKHIERKHENPQKQYVCNFEGCKKAFKKHQQLRTHQCQHTNEPLFRCTHEGCGKHFASPSRLKRHGKVHEGYLCQKGCSFVGKTWTELLKHTREAHKEEVTCTVCQKMFKRKDHLKQHMKTHAPERDVYRCPREGCARTYTTVFNLQSHILSFHEEKRPFVCEHAGCGKTFAMKQSLMRHSVVHDPDKKRMKLKVRPPRERRSLASRLSGYVPPKGKQEPDCSLPNSTESSSSPEATMLAPAALLTVH

Target ORF information:

RefSeq Version	NM_001113570.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus general transcription factor III A (Gtf3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113570.1

ORF Insert Sequence:

CTGGAGCCGC GGGTCTCGGT CGCGGAAGCG GTGTCGTCCC TGACCATCGC GGACGCGTTC 
GTTCGGGCCT GTGTGGGCCC CGCCCCGCCG CGCCCCGCGC TCCCCAGCAG GTTCATCTGC 
TCCTTTCCTG ACTGCAGCGC TAGTTACAAC AAAGCCTGGA AGCTAGACGC GCACCTCTGC 
AAGCACACGG GGGAGAGGCC GTTCGTTTGT GACTATGAGG GCTGTGGCAA AGCCTTCATC 
AGGGACTACC ATCTGAGTCG ACACATCCTG ATTCACACCG GAGAAAAGCC ATTTGTTTGT 
GCAGATAATG GCTGTAATCA GAAATTCAGC ACAAAGTCAA ACTTGAAGAA ACACATTGAA 
CGCAAACATG AAAATCCACA AAAACAGTAT GTGTGCAATT TTGAAGGTTG CAAGAAGGCC 
TTTAAGAAGC ACCAGCAGCT GAGAACCCAT CAGTGCCAGC ACACAAATGA GCCACTCTTC 
AGGTGTACCC ACGAGGGATG TGGGAAGCAC TTTGCCTCCC CCAGCAGGCT GAAACGGCAT 
GGGAAGGTTC ACGAGGGCTA CCTATGTCAA AAGGGATGTT CCTTTGTGGG AAAAACGTGG 
ACAGAGCTTC TGAAGCACAC GAGAGAAGCC CATAAAGAGG AGGTGACCTG CACAGTGTGC 
CAGAAGATGT TCAAGCGCAA AGATCACCTT AAGCAGCACA TGAAGACTCA CGCTCCGGAG 
AGGGATGTGT ACCGCTGCCC GCGGGAAGGC TGTGCAAGGA CCTACACCAC CGTGTTCAAC 
CTGCAGAGCC ACATTCTCTC CTTCCACGAG GAAAAGCGCC CATTTGTGTG TGAGCACGCT 
GGCTGTGGCA AGACGTTTGC AATGAAACAA AGTCTCATGA GGCACAGTGT CGTGCACGAT 
CCTGACAAGA AGAGGATGAA GCTCAAAGTT AGACCACCTC GGGAGAGACG CAGCTTGGCC 
TCGCGCCTCA GCGGGTACGT CCCTCCTAAG GGGAAACAAG AGCCCGACTG CTCCTTGCCT 
AACAGCACAG AGTCCAGCAG CAGCCCAGAG GCCACGATGC TCGCCCCAGC TGCGTTACTT 
ACTGTCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The 5S RNP couples p53 homeostasis to ribosome biogenesis and nucleolar stress.
Cell reports5(1)237-47(2013 Oct)
Sloan KE,Bohnsack MT,Watkins NJ

A structural perspective on RNA polymerase I and RNA polymerase III transcription machineries.
Biochimica et biophysica acta1829(3-4)258-64(2013 Mar-Apr)
Vannini A

cDNA cloning, DNA binding, and evolution of mammalian transcription factor IIIA.
Gene282(1-2)43-52(2002 Jan)
Hanas JS,Hocker JR,Cheng YG,Lerner MR,Brackett DJ,Lightfoot SA,Hanas RJ,Madhusudhan KT,Moreland RJ

Identification of a transcription factor IIIA-interacting protein.
Nucleic acids research28(9)1986-93(2000 May)
Moreland RJ,Dresser ME,Rodgers JS,Roe BA,Conaway JW,Conaway RC,Hanas JS

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Gtf3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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