Pah cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pah
Entrez Gene ID	24616
Full Name	phenylalanine hydroxylase
General protein information	Preferred Names phenylalanine hydroxylase Names phenylalanine-4-hydroxylase phe-4-monooxygenase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q13
Summary	catalyzes the conversion of phenylalanine to tyrosine; deficiency in humans causes genetic disorder phenylketonuria [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012619.2	NP_036751.2	phenylalanine-4-hydroxylase
XM_008765215.2	XP_008763437.1	phenylalanine-4-hydroxylase isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PAH	XP_001156919.1
Rattus norvegicus (Norway rat)	Pah	NP_036751.2
Danio rerio (zebrafish)	pah	NP_956845.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005712	XP_315722.4
Caenorhabditis elegans (roundworm)	pah-1	NP_001254185.1
Xenopus tropicalis (tropical clawed frog)	pah	NP_001027484.1
Bos taurus (cattle)	PAH	NP_001039523.1
Mus musculus (house mouse)	Pah	NP_032803.2
	PAH	XP_001094859.1
Canis lupus familiaris (dog)	PAH	XP_532671.3
Homo sapiens (human)	PAH	NP_000268.1
Gallus gallus (chicken)	PAH	NP_001001298.1
Drosophila melanogaster (fruit fly)	Hn	NP_001261542.1

Gene Ontology
Bibliography

Related articles in PubMed

Domain Movements upon Activation of Phenylalanine Hydroxylase Characterized by Crystallography and Chromatography-Coupled Small-Angle X-ray Scattering.
Meisburger SP, Taylor AB, Khan CA, Zhang S, Fitzpatrick PF, Ando N
Journal of the American Chemical Society138(20)6506-16(2016 May)

Identification of the Allosteric Site for Phenylalanine in Rat Phenylalanine Hydroxylase.
Zhang S, Fitzpatrick PF
The Journal of biological chemistry291(14)7418-25(2016 Apr)

First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer.
Arturo EC, Gupta K, H?roux A, Stith L, Cross PJ, Parker EJ, Loll PJ, Jaffe EK
Proceedings of the National Academy of Sciences of the United States of America113(9)2394-9(2016 Mar)

The Amino Acid Specificity for Activation of Phenylalanine Hydroxylase Matches the Specificity for Stabilization of Regulatory Domain Dimers.
Zhang S, Hinck AP, Fitzpatrick PF
Biochemistry54(33)5167-74(2015 Aug)

Activation of phenylalanine hydroxylase by phenylalanine does not require binding in the active site.
Roberts KM, Khan CA, Hinck CS, Fitzpatrick PF
Biochemistry53(49)7846-53(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results identify the location of the allosteric site as the interface of the regulatory domain dimer formed in activated PheH.
Title: Identification of the Allosteric Site for Phenylalanine in Rat Phenylalanine Hydroxylase.

use of SAXS and X-ray crystallography together to inspect PAH structure provides, to our knowledge, the first complete view of the enzyme in a tetrameric form that was not possible with prior partial crystal structures
Title: First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer.

allosteric activation of phenylalanine hydroxylase is linked to dimerization of regulatory domains
Title: The Amino Acid Specificity for Activation of Phenylalanine Hydroxylase Matches the Specificity for Stabilization of Regulatory Domain Dimers.

Data suggest that phenylalanine [concentration-dependent] activation of phenylalanine hydroxylase does not involve the active site; mutating the active-site residue Arg270 to lysine abolishes phenylalanine-dependent enzyme activation.
Title: Activation of phenylalanine hydroxylase by phenylalanine does not require binding in the active site.

the conformational change upon phosphorylation of phenylalanine hydroxylase is different from and less extensive than that upon phenylalanine activation.
Title: Regulation of phenylalanine hydroxylase: conformational changes upon phosphorylation detected by H/D exchange and mass spectrometry.

The following Pah gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pah cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12084	NM_012619.2 Latest version!	Rattus norvegicus phenylalanine hydroxylase (Pah), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa30031	XM_008765215.2 Latest version!	Rattus norvegicus phenylalanine hydroxylase (Pah), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pah ( NM_012619.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036751.2 Rattus norvegicus phenylalanine hydroxylase (Pah), mRNA.

Price & Availability↑

CloneID	ORa12084
Clone ID Related Accession (Same CDS sequence)	NM_012619.2
Accession Version	NM_012619.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	phenylalanine-4-hydroxylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078881.1. On Oct 13, 2007 this sequence version replaced NM_012619.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078881.1, M12337.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCTG TTGTCCTGGA GAATGGAGTC CTGAGCAGAA AACTCTCAGA CTTTGGGCAG 
GAAACAAGCT ATATTGAAGA CAACTCCAAT CAAAATGGTG CCATATCTCT GATATTCTCA 
CTCAAAGAGG AAGTTGGTGC CCTAGCCAAG GTCCTGCGCT TATTTGAGGA GAACGATATC 
AACCTGACAC ACATTGAATC CAGACCTTCT CGTTTAAACA AAGATGAGTA TGAGTTCTTC 
ACTTATCTGG ATAAGCGTAG CAAACCTGTT CTGGGCAGCA TCATCAAGAG CCTGAGGAAT 
GACATTGGTG CCACTGTCCA CGAGCTTTCC CGAGACAAGG AAAAGAACAC AGTGCCCTGG 
TTCCCAAGGA CCATTCAGGA GTTGGACCGA TTCGCCAATC AGATTCTCAG CTATGGAGCC 
GAACTGGATG CGGACCACCC AGGCTTTAAA GATCCTGTGT ACCGGGCGAG ACGAAAGCAG 
TTTGCTGACA TTGCCTACAA CTACCGCCAT GGCCAGCCCA TCCCTCGGGT GGAATACACA 
GAGGAAGAGA AGCAGACCTG GGGAACGGTG TTCAGGACCT TGAAGGCCTT GTATAAAACA 
CATGCCTGCT ACGAGCACAA CCACATTTTC CCACTTCTGG AAAAGTACTG CGGTTTCCGT 
GAAGACAACA TTCCACAGCT GGAAGATGTT TCTCAATTTC TGCAGACTTG TACTGGTTTC 
CGCCTCCGAC CTGTTGCTGG CTTACTGTCA TCTCGAGATT TCTTGGGTGG CCTGGCCTTC 
CGAGTCTTCC ACTGCACACA GTACATTAGG CATGGATCGA AGCCCATGTA CACACCTGAA 
CCTGACATCT GCCATGAACT CTTGGGACAT GTGCCTTTGT TTTCAGATCG CAGCTTTGCC 
CAGTTTTCTC AGGAAATTGG GCTTGCATCA CTGGGGGCAC CTGATGAGTA CATTGAGAAA 
CTGGCCACAA TTTACTGGTT TACTGTGGAG TTTGGGCTTT GCAAGGAAGG AGATTCTATA 
AAGGCATATG GTGCTGGGCT CTTGTCATCC TTTGGAGAAT TACAGTACTG TTTATCAGAC 
AAGCCAAAGC TCCTCCCCCT GGAGCTAGAG AAGATAGCCT GCCAGGAGTA TTCTGTCACA 
GAGTTCCAGC CCTTGTACTA CGTGGCGGAG AGTTTCAGTG ATGCCAAGGA GAAAGTGAGG 
ACTTTTGCTG CCACGATCCC TCGGCCCTTC TCGGTTCGCT ATGACCCTTA CACTCAAAGG 
GTTGAGGTCC TGGACAATAC TCAGCAGTTG AAGATTTTAG CTGACTCCAT TAATAGTGAG 
GTCGGAATCC TTTGCAATGC CCTGCAGAAG ATAAAGTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036751.2
CDS	90..1451
Translation	MAAVVLENGVLSRKLSDFGQETSYIEDNSNQNGAISLIFSLKEEVGALAKVLRLFEENDINLTHIESRPSRLNKDEYEFFTYLDKRSKPVLGSIIKSLRNDIGATVHELSRDKEKNTVPWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYTEEEKQTWGTVFRTLKALYKTHACYEHNHIFPLLEKYCGFREDNIPQLEDVSQFLQTCTGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKEGDSIKAYGAGLLSSFGELQYCLSDKPKLLPLELEKIACQEYSVTEFQPLYYVAESFSDAKEKVRTFAATIPRPFSVRYDPYTQRVEVLDNTQQLKILADSINSEVGILCNALQKIKS

Target ORF information:

RefSeq Version	NM_012619.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phenylalanine hydroxylase (Pah), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012619.2

ORF Insert Sequence:

ATGGCAGCTG TTGTCCTGGA GAATGGAGTC CTGAGCAGAA AACTCTCAGA CTTTGGGCAG 
GAAACAAGCT ATATTGAAGA CAACTCCAAT CAAAATGGTG CCATATCTCT GATATTCTCA 
CTCAAAGAGG AAGTTGGTGC CCTAGCCAAG GTCCTGCGCT TATTTGAGGA GAACGATATC 
AACCTGACAC ACATTGAATC CAGACCTTCT CGTTTAAACA AAGATGAGTA TGAGTTCTTC 
ACTTATCTGG ATAAGCGTAG CAAACCTGTT CTGGGCAGCA TCATCAAGAG CCTGAGGAAT 
GACATTGGTG CCACTGTCCA CGAGCTTTCC CGAGACAAGG AAAAGAACAC AGTGCCCTGG 
TTCCCAAGGA CCATTCAGGA GTTGGACCGA TTCGCCAATC AGATTCTCAG CTATGGAGCC 
GAACTGGATG CGGACCACCC AGGCTTTAAA GATCCTGTGT ACCGGGCGAG ACGAAAGCAG 
TTTGCTGACA TTGCCTACAA CTACCGCCAT GGCCAGCCCA TCCCTCGGGT GGAATACACA 
GAGGAAGAGA AGCAGACCTG GGGAACGGTG TTCAGGACCT TGAAGGCCTT GTATAAAACA 
CATGCCTGCT ACGAGCACAA CCACATTTTC CCACTTCTGG AAAAGTACTG CGGTTTCCGT 
GAAGACAACA TTCCACAGCT GGAAGATGTT TCTCAATTTC TGCAGACTTG TACTGGTTTC 
CGCCTCCGAC CTGTTGCTGG CTTACTGTCA TCTCGAGATT TCTTGGGTGG CCTGGCCTTC 
CGAGTCTTCC ACTGCACACA GTACATTAGG CATGGATCGA AGCCCATGTA CACACCTGAA 
CCTGACATCT GCCATGAACT CTTGGGACAT GTGCCTTTGT TTTCAGATCG CAGCTTTGCC 
CAGTTTTCTC AGGAAATTGG GCTTGCATCA CTGGGGGCAC CTGATGAGTA CATTGAGAAA 
CTGGCCACAA TTTACTGGTT TACTGTGGAG TTTGGGCTTT GCAAGGAAGG AGATTCTATA 
AAGGCATATG GTGCTGGGCT CTTGTCATCC TTTGGAGAAT TACAGTACTG TTTATCAGAC 
AAGCCAAAGC TCCTCCCCCT GGAGCTAGAG AAGATAGCCT GCCAGGAGTA TTCTGTCACA 
GAGTTCCAGC CCTTGTACTA CGTGGCGGAG AGTTTCAGTG ATGCCAAGGA GAAAGTGAGG 
ACTTTTGCTG CCACGATCCC TCGGCCCTTC TCGGTTCGCT ATGACCCTTA CACTCAAAGG 
GTTGAGGTCC TGGACAATAC TCAGCAGTTG AAGATTTTAG CTGACTCCAT TAATAGTGAG 
GTCGGAATCC TTTGCAATGC CCTGCAGAAG ATAAAGTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pah ( XM_008765215.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763437.1 Rattus norvegicus phenylalanine hydroxylase (Pah), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30031
Clone ID Related Accession (Same CDS sequence)	XM_008765215.2
Accession Version	XM_008765215.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phenylalanine-4-hydroxylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765215.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAT GTGCCTCCGG TCATTCTTAC CTCTGGACTG GAATTCGTCT CCACTGGGTC 
TCTTTTCTCA AGATTCCTGT CTTATTCCAA GTGCCCTGGT TCCCAAGGAC CATTCAGGAG 
TTGGACCGAT TCGCCAATCA GATTCTCAGC TATGGAGCCG AACTGGATGC GGACCACCCA 
GGCTTTAAAG ATCCTGTGTA CCGGGCGAGA CGAAAGCAGT TTGCTGACAT TGCCTACAAC 
TACCGCCATG GCCAGCCCAT CCCTCGGGTG GAATACACAG AGGAAGAGAA GCAGACCTGG 
GGAACGGTGT TCAGGACCTT GAAGGCCTTG TATAAAACAC ATGCCTGCTA CGAGCACAAC 
CACATTTTCC CACTTCTGGA AAAGTACTGC GGTTTCCGTG AAGACAACAT TCCACAGCTG 
GAAGATGTTT CTCAATTTCT GCAGACTTGT ACTGGTTTCC GCCTCCGACC TGTTGCTGGC 
TTACTGTCAT CTCGAGATTT CTTGGGTGGC CTGGCCTTCC GAGTCTTCCA CTGCACACAG 
TACATTAGGC ATGGATCGAA GCCCATGTAC ACACCTGAAC CTGACATCTG CCATGAACTC 
TTGGGACATG TGCCTTTGTT TTCAGATCGC AGCTTTGCCC AGTTTTCTCA GGAAATTGGG 
CTTGCATCAC TGGGGGCACC TGATGAGTAC ATTGAGAAAC TGGCCACAAT TTACTGGTTT 
ACTGTGGAGT TTGGGCTTTG CAAGGAAGGA GATTCTATAA AGGCATATGG TGCTGGGCTC 
TTGTCATCCT TTGGAGAATT ACAGTACTGT TTATCAGACA AGCCAAAGCT CCTCCCCCTG 
GAGCTAGAGA AGATAGCCTG CCAGGAGTAT TCTGTCACAG AGTTCCAGCC CTTGTACTAC 
GTGGCGGAGA GTTTCAGTGA TGCCAAGGAG AAAGTGAGGA CTTTTGCTGC CACGATCCCT 
CGGCCCTTCT CGGTTCGCTA TGACCCTTAC ACTCAAAGGG TTGAGGTCCT GGACAATACT 
CAGCAGTTGA AGATTTTAGC TGACTCCATT AATAGTGAGG TCGGAATCCT TTGCAATGCC 
CTGCAGAAGA TAAAGTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763437.1
CDS	300..1400
Translation	MKSCASGHSYLWTGIRLHWVSFLKIPVLFQVPWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYTEEEKQTWGTVFRTLKALYKTHACYEHNHIFPLLEKYCGFREDNIPQLEDVSQFLQTCTGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKEGDSIKAYGAGLLSSFGELQYCLSDKPKLLPLELEKIACQEYSVTEFQPLYYVAESFSDAKEKVRTFAATIPRPFSVRYDPYTQRVEVLDNTQQLKILADSINSEVGILCNALQKIKS

Target ORF information:

RefSeq Version	XM_008765215.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phenylalanine hydroxylase (Pah), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765215.2

ORF Insert Sequence:

ATGAAGTCAT GTGCCTCCGG TCATTCTTAC CTCTGGACTG GAATTCGTCT CCACTGGGTC 
TCTTTTCTCA AGATTCCTGT CTTATTCCAA GTGCCCTGGT TCCCAAGGAC CATTCAGGAG 
TTGGACCGAT TCGCCAATCA GATTCTCAGC TATGGAGCCG AACTGGATGC GGACCACCCA 
GGCTTTAAAG ATCCTGTGTA CCGGGCGAGA CGAAAGCAGT TTGCTGACAT TGCCTACAAC 
TACCGCCATG GCCAGCCCAT CCCTCGGGTG GAATACACAG AGGAAGAGAA GCAGACCTGG 
GGAACGGTGT TCAGGACCTT GAAGGCCTTG TATAAAACAC ATGCCTGCTA CGAGCACAAC 
CACATTTTCC CACTTCTGGA AAAGTACTGC GGTTTCCGTG AAGACAACAT TCCACAGCTG 
GAAGATGTTT CTCAATTTCT GCAGACTTGT ACTGGTTTCC GCCTCCGACC TGTTGCTGGC 
TTACTGTCAT CTCGAGATTT CTTGGGTGGC CTGGCCTTCC GAGTCTTCCA CTGCACACAG 
TACATTAGGC ATGGATCGAA GCCCATGTAC ACACCTGAAC CTGACATCTG CCATGAACTC 
TTGGGACATG TGCCTTTGTT TTCAGATCGC AGCTTTGCCC AGTTTTCTCA GGAAATTGGG 
CTTGCATCAC TGGGGGCACC TGATGAGTAC ATTGAGAAAC TGGCCACAAT TTACTGGTTT 
ACTGTGGAGT TTGGGCTTTG CAAGGAAGGA GATTCTATAA AGGCATATGG TGCTGGGCTC 
TTGTCATCCT TTGGAGAATT ACAGTACTGT TTATCAGACA AGCCAAAGCT CCTCCCCCTG 
GAGCTAGAGA AGATAGCCTG CCAGGAGTAT TCTGTCACAG AGTTCCAGCC CTTGTACTAC 
GTGGCGGAGA GTTTCAGTGA TGCCAAGGAG AAAGTGAGGA CTTTTGCTGC CACGATCCCT 
CGGCCCTTCT CGGTTCGCTA TGACCCTTAC ACTCAAAGGG TTGAGGTCCT GGACAATACT 
CAGCAGTTGA AGATTTTAGC TGACTCCATT AATAGTGAGG TCGGAATCCT TTGCAATGCC 
CTGCAGAAGA TAAAGTCGTG A