Cklf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cklf
Entrez Gene ID	245978
Full Name	chemokine-like factor
Synonyms	Cklf1
General protein information	Preferred Names chemokine-like factor Names chemokine-like factor chemokine-like factor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19p14
Summary	cytokine which plays a role in immune and inflammatory responses; may play a role in skeletal muscle regeneration [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008772240.2	XP_008770462.1	chemokine-like factor isoform X1
NM_139111.1	NP_620811.1	chemokine-like factor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CKLF	NP_058647.1
Mus musculus (house mouse)	Cklf	NP_083571.1
Rattus norvegicus (Norway rat)	Cklf	NP_620811.1
Canis lupus familiaris (dog)	CKLF	NP_001239269.1
Pan troglodytes (chimpanzee)	CKLF	XP_001158562.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of chemokine-like factor 1 improves blood-brain barrier dysfunction in rats following focal cerebral ischemia.
Kong LL, Wang ZY, Hu JF, Yuan YH, Li H, Chen NH
Neuroscience letters627192-8(2016 Aug)

Chemokine-like factor 1 promotes the migration of rat primary cortical neurons by the induction of actin polymerization.
Wang ZZ, Yuan YH, Zhang Y, Wang XF, Chu SF, Han N, Chen NH
Neuroreport25(15)1221-6(2014 Oct)

Inhibition of chemokine-like factor 1 protects against focal cerebral ischemia through the promotion of energy metabolism and anti-apoptotic effect.
Kong LL, Wang ZY, Hu JF, Yuan YH, Han N, Li H, Chen NH
Neurochemistry international7691-8(2014 Oct)

Antagonistic effect of C19 on migration of vascular smooth muscle cells and intimal hyperplasia induced by chemokine-like factor 1.
Zhang T, Qiao Z, Chen F, Zhang X, Xiong J, Jia X, Chen J, Shen C, Guo W
Molecular biology reports40(4)2939-46(2013 Apr)

Expression of chemokine-like factor 1 after focal cerebral ischemia in the rat.
Kong LL, Hu JF, Zhang W, Yuan YH, Ma KL, Han N, Chen NH
Neuroscience letters505(1)14-8(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Chemokine-like factor 1 (CKLF1) is involved in blood-brain barrier disruption after reperfusion. Inhibition of CKLF1 protects against cerebral ischemia by maintaining BBB integrity, possibly via inhibiting the expression of aquaporin-4 and matrix metalloproteinase-9, and increasing the expression of tight junction protein.
Title: Inhibition of chemokine-like factor 1 improves blood-brain barrier dysfunction in rats following focal cerebral ischemia.

Results suggest that CKLF1 promotes the migration of rat primary cerebral cortical neurons by the induction of actin polymerization
Title: Chemokine-like factor 1 promotes the migration of rat primary cortical neurons by the induction of actin polymerization.

results suggest that CKLF1 is associated with neuronal apoptosis after cerebral ischemia and reperfusion
Title: Inhibition of chemokine-like factor 1 protects against focal cerebral ischemia through the promotion of energy metabolism and anti-apoptotic effect.

These data suggest that CKLF1 has a pivotal role in intimal hyperplasia by mediating VSMC migration. C19 was demonstrated to inhibit CKLF1-mediatated chemotaxis
Title: Antagonistic effect of C19 on migration of vascular smooth muscle cells and intimal hyperplasia induced by chemokine-like factor 1.

This study demonstrated that the expression of CKLF1 increases after focal cerebral ischemia in rat brain.
Title: Expression of chemokine-like factor 1 after focal cerebral ischemia in the rat.

The following Cklf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cklf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02204	NM_139111.1 Latest version!	Rattus norvegicus chemokine-like factor (Cklf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa44405	XM_008772240.2 Latest version!	Rattus norvegicus chemokine-like factor (Cklf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cklf ( NM_139111.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620811.1 Rattus norvegicus chemokine-like factor (Cklf), mRNA.

Price & Availability↑

CloneID	ORa02204
Clone ID Related Accession (Same CDS sequence)	NM_139111.1
Accession Version	NM_139111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	chemokine-like factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF253065.2. ##Evidence-Data-START## Transcript exon combination :: AF253065.2, FQ222916.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGACTCTC CACAGAAGGT CGTAGACCAT CAGCCCTTCT GCCTCAGTCT GAAATGCTTT 
GTGAAGACGC TGCGGTTGGT GGTAACTGTG GCCTCTATGA TCTTTTTCAT TGTGGCACAA 
GCCCCTGAAC CGTATATCGT TATCACTGGG TTTGAAGTCA CCATCATTCT CTTTCTCATA 
GCCTTATATA TGTGCAGTCT TGACAAGACA ATGAGATCTT TCTTTTGGCC TTTGCTTGAT 
GTTATCAACT CGGTGGTAAC AACACTATTC ATGCTTATTG TGTCTGTGTC GGCTCTAATA 
CCAGAAACCT CAACAATGAT AATGGTTGGA GGGGTGTTTG GTTTCCTGAC AGTAATATGT 
ACTGTTGCTG ATTGTGCCCT TATGTGCCAG AAACTCCGGT TTCGTCCACA TGGACCTTAT 
CAGAACAGGT CTGCTACTGA TGTAGATGAC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620811.1
CDS	167..622
Translation	MDSPQKVVDHQPFCLSLKCFVKTLRLVVTVASMIFFIVAQAPEPYIVITGFEVTIILFLIALYMCSLDKTMRSFFWPLLDVINSVVTTLFMLIVSVSALIPETSTMIMVGGVFGFLTVICTVADCALMCQKLRFRPHGPYQNRSATDVDDS

Target ORF information:

RefSeq Version	NM_139111.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chemokine-like factor (Cklf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139111.1

ORF Insert Sequence:

ATGGACTCTC CACAGAAGGT CGTAGACCAT CAGCCCTTCT GCCTCAGTCT GAAATGCTTT 
GTGAAGACGC TGCGGTTGGT GGTAACTGTG GCCTCTATGA TCTTTTTCAT TGTGGCACAA 
GCCCCTGAAC CGTATATCGT TATCACTGGG TTTGAAGTCA CCATCATTCT CTTTCTCATA 
GCCTTATATA TGTGCAGTCT TGACAAGACA ATGAGATCTT TCTTTTGGCC TTTGCTTGAT 
GTTATCAACT CGGTGGTAAC AACACTATTC ATGCTTATTG TGTCTGTGTC GGCTCTAATA 
CCAGAAACCT CAACAATGAT AATGGTTGGA GGGGTGTTTG GTTTCCTGAC AGTAATATGT 
ACTGTTGCTG ATTGTGCCCT TATGTGCCAG AAACTCCGGT TTCGTCCACA TGGACCTTAT 
CAGAACAGGT CTGCTACTGA TGTAGATGAC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cklf ( XM_008772240.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770462.1 Rattus norvegicus chemokine-like factor (Cklf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa44405
Clone ID Related Accession (Same CDS sequence)	XM_008772240.2
Accession Version	XM_008772240.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	chemokine-like factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772240.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTC CACAGAAGGT CGTAGACCAT CAGCCCTTCT GCCTCAGTCT GAAATGCTTT 
GTGAAGACGC TGCGGTTGGT GGTAACTGTG GCCTCTATGA TCTTTTTCAT CGTGGCACAA 
GCCCCTGAAC CGTATATCGT TATCACTGGG TTTGAAGTCA CCATCATTCT CTTTCTCATA 
GCCTTATATA TGTGCAGTCT TGACAAGACA ATGAGATCTT TCTTTTGGCC TTTGCTTGTC 
TCATTTCAAT GTGAGTGCCG GCTAGTTTCA TGTCGATTTG ACCCAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770462.1
CDS	160..450
Translation	MDSPQKVVDHQPFCLSLKCFVKTLRLVVTVASMIFFIVAQAPEPYIVITGFEVTIILFLIALYMCSLDKTMRSFFWPLLVSFQCECRLVSCRFDPS

Target ORF information:

RefSeq Version	XM_008772240.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chemokine-like factor (Cklf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772240.2

ORF Insert Sequence:

ATGGACTCTC CACAGAAGGT CGTAGACCAT CAGCCCTTCT GCCTCAGTCT GAAATGCTTT 
GTGAAGACGC TGCGGTTGGT GGTAACTGTG GCCTCTATGA TCTTTTTCAT CGTGGCACAA 
GCCCCTGAAC CGTATATCGT TATCACTGGG TTTGAAGTCA CCATCATTCT CTTTCTCATA 
GCCTTATATA TGTGCAGTCT TGACAAGACA ATGAGATCTT TCTTTTGGCC TTTGCTTGTC 
TCATTTCAAT GTGAGTGCCG GCTAGTTTCA TGTCGATTTG ACCCAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of chemokine-like factor 1 improves blood-brain barrier dysfunction in rats following focal cerebral ischemia.
Neuroscience letters627192-8(2016 Aug)
Kong LL,Wang ZY,Hu JF,Yuan YH,Li H,Chen NH

Chemokine-like factor 1 promotes the migration of rat primary cortical neurons by the induction of actin polymerization.
Neuroreport25(15)1221-6(2014 Oct)
Wang ZZ,Yuan YH,Zhang Y,Wang XF,Chu SF,Han N,Chen NH

Inhibition of chemokine-like factor 1 protects against focal cerebral ischemia through the promotion of energy metabolism and anti-apoptotic effect.
Neurochemistry international7691-8(2014 Oct)
Kong LL,Wang ZY,Hu JF,Yuan YH,Han N,Li H,Chen NH

Antagonistic effect of C19 on migration of vascular smooth muscle cells and intimal hyperplasia induced by chemokine-like factor 1.
Molecular biology reports40(4)2939-46(2013 Apr)
Zhang T,Qiao Z,Chen F,Zhang X,Xiong J,Jia X,Chen J,Shen C,Guo W

Expression of chemokine-like factor 1 after focal cerebral ischemia in the rat.
Neuroscience letters505(1)14-8(2011 Nov)
Kong LL,Hu JF,Zhang W,Yuan YH,Ma KL,Han N,Chen NH