Idh1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Idh1
Entrez Gene ID	24479
Full Name	isocitrate dehydrogenase (NADP(+)) 1, cytosolic
General protein information	Preferred Names isocitrate dehydrogenase (NADP(+)) 1, cytosolic Names isocitrate dehydrogenase [NADP] cytoplasmic IDH IDP Isocitrate dehydrogenase 1, soluble NADP(+)-specific ICDH cytosolic NADP-isocitrate dehydrogenase isocitrate dehydrogenase 1 (NADP+), soluble oxalosuccinate decarboxylase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q32
Summary	catalyzes oxidative decarboxylation of citrate; cytosolic NADP+-dependent isozyme [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006245049.3	XP_006245111.1	isocitrate dehydrogenase [NADP] cytoplasmic isoform X1
NM_031510.1	NP_113698.1	isocitrate dehydrogenase [NADP] cytoplasmic
XM_008767076.2	XP_008765298.1	isocitrate dehydrogenase [NADP] cytoplasmic isoform X1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	idh-1	NP_001255392.1
Schizosaccharomyces pombe (fission yeast)	idp1	NP_594105.2
Magnaporthe oryzae (rice blast fungus)	MGG_07268	XP_003715491.1
Neurospora crassa	NCU03857	XP_955850.1
Homo sapiens (human)	IDH1	NP_005887.2
Drosophila melanogaster (fruit fly)	Idh	NP_652044.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006660	XP_316694.4
Saccharomyces cerevisiae (baker's yeast)	IDP1	NP_010217.1
	IDH1	XP_001107934.1
Gallus gallus (chicken)	IDH1	XP_004942738.1
Eremothecium gossypii	AGOS_AER061C	NP_984921.1
Canis lupus familiaris (dog)	IDH1	XP_536047.2
Bos taurus (cattle)	IDH1	NP_851355.2
Mus musculus (house mouse)	Idh1	NP_034627.3
Danio rerio (zebrafish)	idh1	NP_958907.1
Oryza sativa (rice)	Os05g0573200	NP_001056381.1
Pan troglodytes (chimpanzee)	IDH1	XP_001141655.1
Rattus norvegicus (Norway rat)	Idh1	NP_113698.1
Kluyveromyces lactis	KLLA0B03355g	XP_451683.1
Arabidopsis thaliana (thale cress)	cICDH	NP_176768.1
Oryza sativa (rice)	Os01g0654500	NP_001043749.1

Gene Ontology
Bibliography

Related articles in PubMed

IDH1 Associated with Neuronal Apoptosis in Adult Rats Brain Following Intracerebral Hemorrhage.
Chen X, Wang H, Yu W, Chen F, Wang G, Shi J, Zhou C
Cellular and molecular neurobiology37(5)831-841(2017 Jul)

IDH1 R132C mutation is detected in clear cell hepatocellular carcinoma by pyrosequencing.
Lee JH, Shin DH, Park WY, Shin N, Kim A, Lee HJ, Kim YK, Choi KU, Kim JY, Yang YI, Lee CH, Sol MY
World journal of surgical oncology15(1)82(2017 Apr)

New IDH1 mutant inhibitors for treatment of acute myeloid leukemia.
Okoye-Okafor UC, Bartholdy B, Cartier J, Gao EN, Pietrak B, Rendina AR, Rominger C, Quinn C, Smallwood A, Wiggall KJ, Reif AJ, Schmidt SJ, Qi H, Zhao H, Joberty G, Faelth-Savitski M, Bantscheff M, Drewes G, Duraiswami C, Brady P, Groy A, Narayanagari SR, Antony-Debre I, Mitchell K, Wang HR, Kao YR, Christopeit M, Carvajal L, Barreyro L, Paietta E, Makishima H, Will B, Concha N, Adams ND, Schwartz B, McCabe MT, Maciejewski J, Verma A, Steidl U
Nature chemical biology11(11)878-86(2015 Nov)

Prognosis and Clinicopathologic Features of Patients With Advanced Stage Isocitrate Dehydrogenase (IDH) Mutant and IDH Wild-Type Intrahepatic Cholangiocarcinoma.
Goyal L, Govindan A, Sheth RA, Nardi V, Blaszkowsky LS, Faris JE, Clark JW, Ryan DP, Kwak EL, Allen JN, Murphy JE, Saha SK, Hong TS, Wo JY, Ferrone CR, Tanabe KK, Chong DQ, Deshpande V, Borger DR, Iafrate AJ, Bardeesy N, Zheng H, Zhu AX
The oncologist20(9)1019-27(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IDHc as a component of the emerging pathways that negatively regulate glucose signaling for insulin secretion
Title: A role for cytosolic isocitrate dehydrogenase as a negative regulator of glucose signaling for insulin secretion in pancreatic ?-cells.

IDH1 mutations are closely associated with glioma progression and chemo-resistance.
Title: Glioma derived isocitrate dehydrogenase-2 mutations induced up-regulation of HIF-1? and ?-catenin signaling: possible impact on glioma cell metastasis and chemo-resistance.

Activity of NADP-dependent isocitrate dehydrogenase increases and the catalytic characteristics of the enzyme isolated from the liver of rats are changed under conditions of apoptosis induction in comparison with these characteristics in health.
Title: Catalytic characteristics of NADP-isocitrate dehydrogenase from the liver of rats in health and after injection of TNF-? and melatonin treatment.

These results together indicate that upregulation of IDPc in response to hyperglycemia might play an essential role in preventing the progression of diabetic nephropathy.
Title: Upregulation of cytosolic NADP+-dependent isocitrate dehydrogenase by hyperglycemia protects renal cells against oxidative stress.

a pyruvate cycling pathway involving ICDc plays an important role in control of glucose-stimulated insulin secretion
Title: A pyruvate cycling pathway involving cytosolic NADP-dependent isocitrate dehydrogenase regulates glucose-stimulated insulin secretion.

The following Idh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Idh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07948	NM_031510.1 Latest version!	Rattus norvegicus isocitrate dehydrogenase (NADP(+)) 1 (Idh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa07948	XM_006245049.3 Latest version!	Rattus norvegicus isocitrate dehydrogenase (NADP(+)) 1, cytosolic (Idh1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa07948	XM_008767076.2 Latest version!	Rattus norvegicus isocitrate dehydrogenase (NADP(+)) 1, cytosolic (Idh1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Idh1 ( NM_031510.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113698.1 Rattus norvegicus isocitrate dehydrogenase (NADP(+)) 1 (Idh1), mRNA.

Price & Availability↑

CloneID	ORa07948
Clone ID Related Accession (Same CDS sequence)	XM_006245049.3 , NM_031510.1 , XM_008767076.2
Accession Version	NM_031510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	isocitrate dehydrogenase [NADP] cytoplasmic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L35317.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L35317.1, FQ214242.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCCAGAA AAATCCATGG CGGTTCTGTG GTGGAAATGC AAGGAGATGA AATGACACGG 
ATCATCTGGG AATTGATTAA AGAAAAGCTC ATTCTTCCCT ACGTGGAACT GGATCTGCAT 
AGCTATGACT TAGGCATAGA GAATCGGGAT GCCACCAATG ACCAGGTCAC CAAGGATGCT 
GCGGAGGCTA TAAAGAAATA CAACGTCGGC GTCAAGTGTG CTACCATCAC CCCCGATGAG 
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCCAACGG TACCATCCGG 
AACATTCTGG GCGGCACTGT CTTCAGAGAA GCTATTATCT GCAAAAATAT CCCCCGGCTA 
GTGACAGGCT GGGTAAAACC CATCATCATT GGCCGACACG CCTATGGGGA CCAATACAGA 
GCAACAGATT TCGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAAAGAT 
GGGTCGCAGA AAGTGACATA CCTGGTGCAT GACTTTGAAG AAGGTGGTGG TGTTGCCATG 
GGTATGTACA ACCAGGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT 
CTGTCGAAGG GCTGGCCTTT GTATCTAAGC ACCAAGAACA CTATTCTGAA GAAGTATGAT 
GGACGCTTCA AAGACATCTT CCAGGAGATC TATGACAAGC AATACAAGTC CAAGTTTGAA 
GCTCAGAAGA TCTGGTATGA ACACAGGCTC ATAGATGACA TGGTGGCCCA GGCTATGAAA 
TCAGAAGGAG GCTTCATCTG GGCCTGTAAG AACTATGATG GTGATGTGCA GTCAGACTCA 
GTAGCCCAAG GTTATGGCTC CCTTGGCATG ATGACCAGTG TGCTGATTTG TCCAGATGGT 
AAGACGGTAG AAGCAGAGGC TGCCCATGGC ACTGTCACAC GTCACTACCG CATGTACCAG 
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGTC CCGAGGGTTA 
GCCCACAGAG CAAAGCTTGA CAACAATACT GAGCTCAGCT TCTTTGCAAA TGCTTTGGAA 
GAAGTCTGCA TTGAGACCAT TGAGGCTGGC TTTATGACTA AGGACTTGGC TGCTTGCATT 
AAAGGCTTAC CCAATGTACA GCGTTCTGAC TACTTGAATA CATTTGAGTT TATGGACAAA 
CTTGGAGAAA ACTTGAAGGC CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113698.1
CDS	30..1274
Translation	MSRKIHGGSVVEMQGDEMTRIIWELIKEKLILPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKYNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPKDGSQKVTYLVHDFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSKFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLICPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWSRGLAHRAKLDNNTELSFFANALEEVCIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKAKLAQAKL

Target ORF information:

RefSeq Version	NM_031510.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus isocitrate dehydrogenase (NADP(+)) 1 (Idh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031510.1

ORF Insert Sequence:

ATGTCCAGAA AAATCCATGG CGGTTCTGTG GTGGAAATGC AAGGAGATGA AATGACACGG 
ATCATCTGGG AATTGATTAA AGAAAAGCTC ATTCTTCCCT ACGTGGAACT GGATCTGCAT 
AGCTATGACT TAGGCATAGA GAATCGGGAT GCCACCAATG ACCAGGTCAC CAAGGATGCT 
GCGGAGGCTA TAAAGAAATA CAACGTCGGC GTCAAGTGTG CTACCATCAC CCCCGATGAG 
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCCAACGG TACCATCCGG 
AACATTCTGG GCGGCACTGT CTTCAGAGAA GCTATTATCT GCAAAAATAT CCCCCGGCTA 
GTGACAGGCT GGGTAAAACC CATCATCATT GGCCGACACG CCTATGGGGA CCAATACAGA 
GCAACAGATT TCGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAAAGAT 
GGGTCGCAGA AAGTGACATA CCTGGTGCAT GACTTTGAAG AAGGTGGTGG TGTTGCCATG 
GGTATGTACA ACCAGGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT 
CTGTCGAAGG GCTGGCCTTT GTATCTAAGC ACCAAGAACA CTATTCTGAA GAAGTATGAT 
GGACGCTTCA AAGACATCTT CCAGGAGATC TATGACAAGC AATACAAGTC CAAGTTTGAA 
GCTCAGAAGA TCTGGTATGA ACACAGGCTC ATAGATGACA TGGTGGCCCA GGCTATGAAA 
TCAGAAGGAG GCTTCATCTG GGCCTGTAAG AACTATGATG GTGATGTGCA GTCAGACTCA 
GTAGCCCAAG GTTATGGCTC CCTTGGCATG ATGACCAGTG TGCTGATTTG TCCAGATGGT 
AAGACGGTAG AAGCAGAGGC TGCCCATGGC ACTGTCACAC GTCACTACCG CATGTACCAG 
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGTC CCGAGGGTTA 
GCCCACAGAG CAAAGCTTGA CAACAATACT GAGCTCAGCT TCTTTGCAAA TGCTTTGGAA 
GAAGTCTGCA TTGAGACCAT TGAGGCTGGC TTTATGACTA AGGACTTGGC TGCTTGCATT 
AAAGGCTTAC CCAATGTACA GCGTTCTGAC TACTTGAATA CATTTGAGTT TATGGACAAA 
CTTGGAGAAA ACTTGAAGGC CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Idh1 ( XM_006245049.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245111.1 Rattus norvegicus isocitrate dehydrogenase (NADP(+)) 1, cytosolic (Idh1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa07948
Clone ID Related Accession (Same CDS sequence)	XM_006245049.3 , NM_031510.1 , XM_008767076.2
Accession Version	XM_006245049.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	isocitrate dehydrogenase [NADP] cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245049.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAA AAATCCATGG CGGTTCTGTG GTGGAAATGC AAGGAGATGA AATGACACGG 
ATCATCTGGG AATTGATTAA AGAAAAGCTC ATTCTTCCCT ACGTGGAACT GGATCTGCAT 
AGCTATGACT TAGGCATAGA GAATCGGGAT GCCACCAATG ACCAGGTCAC CAAGGATGCT 
GCGGAGGCTA TAAAGAAATA CAACGTCGGC GTCAAGTGTG CTACCATCAC CCCCGATGAG 
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCCAACGG TACCATCCGG 
AACATTCTGG GCGGCACTGT CTTCAGAGAA GCTATTATCT GCAAAAATAT CCCCCGGCTA 
GTGACAGGCT GGGTAAAACC CATCATCATT GGCCGACACG CCTATGGGGA CCAATACAGA 
GCAACAGATT TCGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAAAGAT 
GGGTCGCAGA AAGTGACATA CCTGGTGCAT GACTTTGAAG AAGGTGGTGG TGTTGCCATG 
GGTATGTACA ACCAGGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT 
CTGTCGAAGG GCTGGCCTTT GTATCTAAGC ACCAAGAACA CTATTCTGAA GAAGTATGAT 
GGACGCTTCA AAGACATCTT CCAGGAGATC TATGACAAGC AATACAAGTC CAAGTTTGAA 
GCTCAGAAGA TCTGGTATGA ACACAGGCTC ATAGATGACA TGGTGGCCCA GGCTATGAAA 
TCAGAAGGAG GCTTCATCTG GGCCTGTAAG AACTATGATG GTGATGTGCA GTCAGACTCA 
GTAGCCCAAG GTTATGGCTC CCTTGGCATG ATGACCAGTG TGCTGATTTG TCCAGATGGT 
AAGACGGTAG AAGCAGAGGC TGCCCATGGC ACTGTCACAC GTCACTACCG CATGTACCAG 
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGTC CCGAGGGTTA 
GCCCACAGAG CAAAGCTTGA CAACAATACT GAGCTCAGCT TCTTTGCAAA TGCTTTGGAA 
GAAGTCTGCA TTGAGACCAT TGAGGCTGGC TTTATGACTA AGGACTTGGC TGCTTGCATT 
AAAGGCTTAC CCAATGTACA GCGTTCTGAC TACTTGAATA CATTTGAGTT TATGGACAAA 
CTTGGAGAAA ACTTGAAGGC CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245111.1
CDS	107..1351
Translation	MSRKIHGGSVVEMQGDEMTRIIWELIKEKLILPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKYNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPKDGSQKVTYLVHDFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSKFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLICPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWSRGLAHRAKLDNNTELSFFANALEEVCIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKAKLAQAKL

Target ORF information:

RefSeq Version	XM_006245049.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus isocitrate dehydrogenase (NADP(+)) 1, cytosolic (Idh1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245049.3

ORF Insert Sequence:

ATGTCCAGAA AAATCCATGG CGGTTCTGTG GTGGAAATGC AAGGAGATGA AATGACACGG 
ATCATCTGGG AATTGATTAA AGAAAAGCTC ATTCTTCCCT ACGTGGAACT GGATCTGCAT 
AGCTATGACT TAGGCATAGA GAATCGGGAT GCCACCAATG ACCAGGTCAC CAAGGATGCT 
GCGGAGGCTA TAAAGAAATA CAACGTCGGC GTCAAGTGTG CTACCATCAC CCCCGATGAG 
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCCAACGG TACCATCCGG 
AACATTCTGG GCGGCACTGT CTTCAGAGAA GCTATTATCT GCAAAAATAT CCCCCGGCTA 
GTGACAGGCT GGGTAAAACC CATCATCATT GGCCGACACG CCTATGGGGA CCAATACAGA 
GCAACAGATT TCGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAAAGAT 
GGGTCGCAGA AAGTGACATA CCTGGTGCAT GACTTTGAAG AAGGTGGTGG TGTTGCCATG 
GGTATGTACA ACCAGGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT 
CTGTCGAAGG GCTGGCCTTT GTATCTAAGC ACCAAGAACA CTATTCTGAA GAAGTATGAT 
GGACGCTTCA AAGACATCTT CCAGGAGATC TATGACAAGC AATACAAGTC CAAGTTTGAA 
GCTCAGAAGA TCTGGTATGA ACACAGGCTC ATAGATGACA TGGTGGCCCA GGCTATGAAA 
TCAGAAGGAG GCTTCATCTG GGCCTGTAAG AACTATGATG GTGATGTGCA GTCAGACTCA 
GTAGCCCAAG GTTATGGCTC CCTTGGCATG ATGACCAGTG TGCTGATTTG TCCAGATGGT 
AAGACGGTAG AAGCAGAGGC TGCCCATGGC ACTGTCACAC GTCACTACCG CATGTACCAG 
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGTC CCGAGGGTTA 
GCCCACAGAG CAAAGCTTGA CAACAATACT GAGCTCAGCT TCTTTGCAAA TGCTTTGGAA 
GAAGTCTGCA TTGAGACCAT TGAGGCTGGC TTTATGACTA AGGACTTGGC TGCTTGCATT 
AAAGGCTTAC CCAATGTACA GCGTTCTGAC TACTTGAATA CATTTGAGTT TATGGACAAA 
CTTGGAGAAA ACTTGAAGGC CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Idh1 ( XM_008767076.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765298.1 Rattus norvegicus isocitrate dehydrogenase (NADP(+)) 1, cytosolic (Idh1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa07948
Clone ID Related Accession (Same CDS sequence)	XM_006245049.3 , NM_031510.1 , XM_008767076.2
Accession Version	XM_008767076.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	isocitrate dehydrogenase [NADP] cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAA AAATCCATGG CGGTTCTGTG GTGGAAATGC AAGGAGATGA AATGACACGG 
ATCATCTGGG AATTGATTAA AGAAAAGCTC ATTCTTCCCT ACGTGGAACT GGATCTGCAT 
AGCTATGACT TAGGCATAGA GAATCGGGAT GCCACCAATG ACCAGGTCAC CAAGGATGCT 
GCGGAGGCTA TAAAGAAATA CAACGTCGGC GTCAAGTGTG CTACCATCAC CCCCGATGAG 
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCCAACGG TACCATCCGG 
AACATTCTGG GCGGCACTGT CTTCAGAGAA GCTATTATCT GCAAAAATAT CCCCCGGCTA 
GTGACAGGCT GGGTAAAACC CATCATCATT GGCCGACACG CCTATGGGGA CCAATACAGA 
GCAACAGATT TCGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAAAGAT 
GGGTCGCAGA AAGTGACATA CCTGGTGCAT GACTTTGAAG AAGGTGGTGG TGTTGCCATG 
GGTATGTACA ACCAGGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT 
CTGTCGAAGG GCTGGCCTTT GTATCTAAGC ACCAAGAACA CTATTCTGAA GAAGTATGAT 
GGACGCTTCA AAGACATCTT CCAGGAGATC TATGACAAGC AATACAAGTC CAAGTTTGAA 
GCTCAGAAGA TCTGGTATGA ACACAGGCTC ATAGATGACA TGGTGGCCCA GGCTATGAAA 
TCAGAAGGAG GCTTCATCTG GGCCTGTAAG AACTATGATG GTGATGTGCA GTCAGACTCA 
GTAGCCCAAG GTTATGGCTC CCTTGGCATG ATGACCAGTG TGCTGATTTG TCCAGATGGT 
AAGACGGTAG AAGCAGAGGC TGCCCATGGC ACTGTCACAC GTCACTACCG CATGTACCAG 
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGTC CCGAGGGTTA 
GCCCACAGAG CAAAGCTTGA CAACAATACT GAGCTCAGCT TCTTTGCAAA TGCTTTGGAA 
GAAGTCTGCA TTGAGACCAT TGAGGCTGGC TTTATGACTA AGGACTTGGC TGCTTGCATT 
AAAGGCTTAC CCAATGTACA GCGTTCTGAC TACTTGAATA CATTTGAGTT TATGGACAAA 
CTTGGAGAAA ACTTGAAGGC CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765298.1
CDS	117..1361
Translation	MSRKIHGGSVVEMQGDEMTRIIWELIKEKLILPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKYNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPKDGSQKVTYLVHDFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSKFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLICPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWSRGLAHRAKLDNNTELSFFANALEEVCIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKAKLAQAKL

Target ORF information:

RefSeq Version	XM_008767076.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus isocitrate dehydrogenase (NADP(+)) 1, cytosolic (Idh1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767076.2

ORF Insert Sequence:

ATGTCCAGAA AAATCCATGG CGGTTCTGTG GTGGAAATGC AAGGAGATGA AATGACACGG 
ATCATCTGGG AATTGATTAA AGAAAAGCTC ATTCTTCCCT ACGTGGAACT GGATCTGCAT 
AGCTATGACT TAGGCATAGA GAATCGGGAT GCCACCAATG ACCAGGTCAC CAAGGATGCT 
GCGGAGGCTA TAAAGAAATA CAACGTCGGC GTCAAGTGTG CTACCATCAC CCCCGATGAG 
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCCAACGG TACCATCCGG 
AACATTCTGG GCGGCACTGT CTTCAGAGAA GCTATTATCT GCAAAAATAT CCCCCGGCTA 
GTGACAGGCT GGGTAAAACC CATCATCATT GGCCGACACG CCTATGGGGA CCAATACAGA 
GCAACAGATT TCGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAAAGAT 
GGGTCGCAGA AAGTGACATA CCTGGTGCAT GACTTTGAAG AAGGTGGTGG TGTTGCCATG 
GGTATGTACA ACCAGGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT 
CTGTCGAAGG GCTGGCCTTT GTATCTAAGC ACCAAGAACA CTATTCTGAA GAAGTATGAT 
GGACGCTTCA AAGACATCTT CCAGGAGATC TATGACAAGC AATACAAGTC CAAGTTTGAA 
GCTCAGAAGA TCTGGTATGA ACACAGGCTC ATAGATGACA TGGTGGCCCA GGCTATGAAA 
TCAGAAGGAG GCTTCATCTG GGCCTGTAAG AACTATGATG GTGATGTGCA GTCAGACTCA 
GTAGCCCAAG GTTATGGCTC CCTTGGCATG ATGACCAGTG TGCTGATTTG TCCAGATGGT 
AAGACGGTAG AAGCAGAGGC TGCCCATGGC ACTGTCACAC GTCACTACCG CATGTACCAG 
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGTC CCGAGGGTTA 
GCCCACAGAG CAAAGCTTGA CAACAATACT GAGCTCAGCT TCTTTGCAAA TGCTTTGGAA 
GAAGTCTGCA TTGAGACCAT TGAGGCTGGC TTTATGACTA AGGACTTGGC TGCTTGCATT 
AAAGGCTTAC CCAATGTACA GCGTTCTGAC TACTTGAATA CATTTGAGTT TATGGACAAA 
CTTGGAGAAA ACTTGAAGGC CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IDH1 Associated with Neuronal Apoptosis in Adult Rats Brain Following Intracerebral Hemorrhage.
Cellular and molecular neurobiology37(5)831-841(2017 Jul)
Chen X,Wang H,Yu W,Chen F,Wang G,Shi J,Zhou C

IDH1 R132C mutation is detected in clear cell hepatocellular carcinoma by pyrosequencing.
World journal of surgical oncology15(1)82(2017 Apr)
Lee JH,Shin DH,Park WY,Shin N,Kim A,Lee HJ,Kim YK,Choi KU,Kim JY,Yang YI,Lee CH,Sol MY

New IDH1 mutant inhibitors for treatment of acute myeloid leukemia.
Nature chemical biology11(11)878-86(2015 Nov)
Okoye-Okafor UC,Bartholdy B,Cartier J,Gao EN,Pietrak B,Rendina AR,Rominger C,Quinn C,Smallwood A,Wiggall KJ,Reif AJ,Schmidt SJ,Qi H,Zhao H,Joberty G,Faelth-Savitski M,Bantscheff M,Drewes G,Duraiswami C,Brady P,Groy A,Narayanagari SR,Antony-Debre I,Mitchell K,Wang HR,Kao YR,Christopeit M,Carvajal L,Barreyro L,Paietta E,Makishima H,Will B,Concha N,Adams ND,Schwartz B,McCabe MT,Maciejewski J,Verma A,Steidl U

Prognosis and Clinicopathologic Features of Patients With Advanced Stage Isocitrate Dehydrogenase (IDH) Mutant and IDH Wild-Type Intrahepatic Cholangiocarcinoma.
The oncologist20(9)1019-27(2015 Sep)
Goyal L,Govindan A,Sheth RA,Nardi V,Blaszkowsky LS,Faris JE,Clark JW,Ryan DP,Kwak EL,Allen JN,Murphy JE,Saha SK,Hong TS,Wo JY,Ferrone CR,Tanabe KK,Chong DQ,Deshpande V,Borger DR,Iafrate AJ,Bardeesy N,Zheng H,Zhu AX

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Idh1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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