Hsd3b5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsd3b5
Entrez Gene ID	24470
Full Name	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5
Synonyms	Hsd1,Hsd3b,RATHSDI
General protein information	Preferred Names hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5 Names 3 beta-hydroxysteroid dehydrogenase type 5 3 beta-hydroxysteroid dehydrogenase 5 3 beta-hydroxysteroid dehydrogenase type V 3-beta-HSD V 3-beta-hydroxy-5-ene steroid dehydrogenase NADPH-dependent 3-beta-hydroxy-Delta(5)-steroid dehydrogenase progesterone reductase steroid delta-isomerase, 3 beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	catalyzes the conversion of delta 5-3 beta-hydroxysteroids to delta 4-3-ketosteroids in steroid hormone biosynthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012584.1	NP_036716.1	NADPH-dependent 3-keto-steroid reductase Hsd3b5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Hsd3b5	NP_036716.1
Mus musculus (house mouse)	Hsd3b5	NP_032321.2
Mus musculus (house mouse)	Gm10681	NP_001257358.1
Mus musculus (house mouse)	Hsd3b4	NP_032320.1
Mus musculus (house mouse)	Gm4450	XP_001480548.2
Rattus norvegicus (Norway rat)	LOC100911116	XP_003749408.1

Gene Ontology
Bibliography

Related articles in PubMed

Crucial role of estrogen receptor-alpha interaction with transcription coregulators in follicle-stimulating hormone and transforming growth factor beta1 up-regulation of steroidogenesis in rat ovarian granulosa cells.
Chen YJ, Lee MT, Yao HC, Hsiao PW, Ke FC, Hwang JJ
Endocrinology149(9)4658-68(2008 Sep)

3beta-Hydroxysteroid dehydrogenase/5-ene-4-ene isomerase mRNA expression in rat brain: effect of pseudopregnancy and traumatic brain injury.
Meffre D, Delespierre B, Gou?zou M, Schumacher M, Stein DG, Guennoun R
The Journal of steroid biochemistry and molecular biology104(3-5)293-300(2007 May)

Direct effects of IL-15 on corticosterone secretion by rat adrenocortical cells.
Lo MJ, Lee SD
Immunology and cell biology84(1)38-43(2006 Feb)

Neurosteroids and female reproduction: estrogen increases 3beta-HSD mRNA and activity in rat hypothalamus.
Soma KK, Sinchak K, Lakhter A, Schlinger BA, Micevych PE
Endocrinology146(10)4386-90(2005 Oct)

Development of a quantitative assay method for 3 beta-hydroxy-delta 5-steroid dehydrogenase in the rat testis.
Qujeq D
Steroids67(13-14)1071-7(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ERalpha interaction with transcription coregulators, histone acetylases (CBP/SRC-1), and PGC-1alpha is crucial to FSH and TGFbeta1-up-regulated expression of Hsd3b and Cyp11a1, and, thus, progesterone production in rat ovarian granulosa cells.
Title: Crucial role of estrogen receptor-alpha interaction with transcription coregulators in follicle-stimulating hormone and transforming growth factor beta1 up-regulation of steroidogenesis in rat ovarian granulosa cells.

Significant decrease in the 3beta-HSD mRNA expression in traumatic brain injury site within the frontal cortex in both males and pseudopregnant females.
Title: 3beta-Hydroxysteroid dehydrogenase/5-ene-4-ene isomerase mRNA expression in rat brain: effect of pseudopregnancy and traumatic brain injury.

In ovariectomized-adrenalectomized rats, estrogen treatment increased 3beta-HSD mRNA in the hypothalamus.
Title: Neurosteroids and female reproduction: estrogen increases 3beta-HSD mRNA and activity in rat hypothalamus.

Expression of Hsd3b throughout the spinal cord even after castration and adrenalectomy suggests a potential endogenous production of progesterone and an important signalling function of this steroid in the spinal cord.
Title: 3 Beta-hydroxysteroid dehydrogenase expression in rat spinal cord.

The following Hsd3b5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsd3b5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42433	NM_012584.1 Latest version!	Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5 (Hsd3b5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsd3b5 ( NM_012584.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036716.1 Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5 (Hsd3b5), mRNA.

Price & Availability↑

CloneID	ORa42433
Clone ID Related Accession (Same CDS sequence)	NM_012584.1
Accession Version	NM_012584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	NADPH-dependent 3-keto-steroid reductase Hsd3b5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M67465.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M67465.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGGGT GGAGCTGCCT GGTGACAGGA GCAGGAGGAT TTCTGGGCCA GAGGATTGTC 
CAAATGTTGG TGCAAGAGAA AGAGCTGCAG GAGGTCAGAG TCCTGTATAG GACCTTCAGT 
CCAAAACACA AAGAGGAACT GTCCAAGCTG CAGACAAAAG CCAAGGTGAC GGTACTGAGG 
GGAGACATTG TGGATGCCCA GTTCCTGAGG AGAGCGTGCC AGGGCATGTC CGTCATCATC 
CACACCGCTG CTGCCCTTGA TATCGCAGGC TTCCTGCCCA GGCAGACCAT CCTAGATGTC 
AATGTGAAAG GTACTCAGCT CCTGTTGGAT GCTTGTGTGG AAGCCAGCGT ACCAGCCTTC 
ATCTACAGCA GCTCAACAGG TGTGGCTGGA CCAAACTCCT ACAAGGAGAC CATCCTGAAC 
GACCGTGAGG AAGAGCATCG TGAAAGCACA TGGTCAAATC CATACCCATA CAGCAAAAGG 
ATGGCCGAGA AGGCAGTGCT GGCAGCCAAT GGGAGCATCC TGAAAAATGG TGGCACTTTT 
CATACATGTG CCTTAAGACT CCCATTCATC TATGGGGAAG AAAGCCAAAT CATTTCAACC 
ATGGTAAATA GAGCACTCAA GAACAACAGC ATAATTAAAA GACATGCCAC ATTCTCCATA 
GCCAACCCAG TGTATGTGGG TAATGCAGCC TGGGCACACA TTCTGGCTGC CAGGGGCCTT 
CGAGACCCCG AGAAGTCACA AAGCATCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAT ACCCTGAGCA AAGAATGGGG CTTCTGCCTT 
GATTCCAGTT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAACT 
GTGAGCTTCC TGCTGCGTCC ATTTTACAAC TATAGGCCAC CCTTTAACCG CTTCATGGTC 
ACGATCTTAA ATAGCGTGTT TACCATTTCC TACAAGAAAG CTCAGAGAGA TCTGGGCTAT 
GAGCCACTTG TCAGCTGGGA GGAAGCCAAG CAAAAAACCT CGGAGTGGAT CGGGACACTA 
GTGGAGCAAC ACAGGGAGAC ACTGGACACA AAGTCTCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036716.1
CDS	85..1206
Translation	MPGWSCLVTGAGGFLGQRIVQMLVQEKELQEVRVLYRTFSPKHKEELSKLQTKAKVTVLRGDIVDAQFLRRACQGMSVIIHTAAALDIAGFLPRQTILDVNVKGTQLLLDACVEASVPAFIYSSSTGVAGPNSYKETILNDREEEHRESTWSNPYPYSKRMAEKAVLAANGSILKNGGTFHTCALRLPFIYGEESQIISTMVNRALKNNSIIKRHATFSIANPVYVGNAAWAHILAARGLRDPEKSQSIQGQFYYISDDTPHQSYDDLNYTLSKEWGFCLDSSWSLPLPLLYWLAFLLETVSFLLRPFYNYRPPFNRFMVTILNSVFTISYKKAQRDLGYEPLVSWEEAKQKTSEWIGTLVEQHRETLDTKSQ

Target ORF information:

RefSeq Version	NM_012584.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5 (Hsd3b5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012584.1

ORF Insert Sequence:

ATGCCTGGGT GGAGCTGCCT GGTGACAGGA GCAGGAGGAT TTCTGGGCCA GAGGATTGTC 
CAAATGTTGG TGCAAGAGAA AGAGCTGCAG GAGGTCAGAG TCCTGTATAG GACCTTCAGT 
CCAAAACACA AAGAGGAACT GTCCAAGCTG CAGACAAAAG CCAAGGTGAC GGTACTGAGG 
GGAGACATTG TGGATGCCCA GTTCCTGAGG AGAGCGTGCC AGGGCATGTC CGTCATCATC 
CACACCGCTG CTGCCCTTGA TATCGCAGGC TTCCTGCCCA GGCAGACCAT CCTAGATGTC 
AATGTGAAAG GTACTCAGCT CCTGTTGGAT GCTTGTGTGG AAGCCAGCGT ACCAGCCTTC 
ATCTACAGCA GCTCAACAGG TGTGGCTGGA CCAAACTCCT ACAAGGAGAC CATCCTGAAC 
GACCGTGAGG AAGAGCATCG TGAAAGCACA TGGTCAAATC CATACCCATA CAGCAAAAGG 
ATGGCCGAGA AGGCAGTGCT GGCAGCCAAT GGGAGCATCC TGAAAAATGG TGGCACTTTT 
CATACATGTG CCTTAAGACT CCCATTCATC TATGGGGAAG AAAGCCAAAT CATTTCAACC 
ATGGTAAATA GAGCACTCAA GAACAACAGC ATAATTAAAA GACATGCCAC ATTCTCCATA 
GCCAACCCAG TGTATGTGGG TAATGCAGCC TGGGCACACA TTCTGGCTGC CAGGGGCCTT 
CGAGACCCCG AGAAGTCACA AAGCATCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAT ACCCTGAGCA AAGAATGGGG CTTCTGCCTT 
GATTCCAGTT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAACT 
GTGAGCTTCC TGCTGCGTCC ATTTTACAAC TATAGGCCAC CCTTTAACCG CTTCATGGTC 
ACGATCTTAA ATAGCGTGTT TACCATTTCC TACAAGAAAG CTCAGAGAGA TCTGGGCTAT 
GAGCCACTTG TCAGCTGGGA GGAAGCCAAG CAAAAAACCT CGGAGTGGAT CGGGACACTA 
GTGGAGCAAC ACAGGGAGAC ACTGGACACA AAGTCTCAAT GA